##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938997_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 583658 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06342241518149 33.0 31.0 34.0 30.0 34.0 2 32.26491369946098 34.0 31.0 34.0 30.0 34.0 3 32.31923832107159 34.0 31.0 34.0 30.0 34.0 4 35.88561794749665 37.0 35.0 37.0 35.0 37.0 5 35.82982328692488 37.0 35.0 37.0 35.0 37.0 6 35.9224854281103 37.0 35.0 37.0 35.0 37.0 7 35.869178525780505 37.0 35.0 37.0 35.0 37.0 8 35.880851457531634 37.0 35.0 37.0 35.0 37.0 9 37.47761017582214 39.0 37.0 39.0 34.0 39.0 10-11 37.40149796627477 39.0 37.0 39.0 34.0 39.0 12-13 37.32733381535077 39.0 37.0 39.0 34.0 39.0 14-15 38.43502787591363 40.0 38.0 41.0 33.5 41.0 16-17 38.52991049552992 40.0 38.0 41.0 34.0 41.0 18-19 38.55402221849097 40.0 38.0 41.0 34.0 41.0 20-21 38.51424035993681 40.0 38.0 41.0 34.0 41.0 22-23 38.41493306011397 40.0 38.0 41.0 34.0 41.0 24-25 38.271766171285236 40.0 37.0 41.0 33.5 41.0 26-27 37.992400515370306 40.0 37.0 41.0 33.0 41.0 28-29 37.790226468239965 40.0 36.0 41.0 33.0 41.0 30-31 37.535277508403894 39.5 36.0 41.0 33.0 41.0 32-33 37.23598665656942 39.0 35.0 41.0 33.0 41.0 34-35 37.03838720620638 39.0 35.0 41.0 32.0 41.0 36-37 36.81052688389434 39.0 35.0 41.0 31.5 41.0 38-39 36.58296468137163 39.0 35.0 41.0 31.0 41.0 40-41 36.39420345476289 39.0 35.0 41.0 31.0 41.0 42-43 36.1459073635588 39.0 35.0 41.0 30.0 41.0 44-45 35.83819719767398 38.0 35.0 41.0 29.5 41.0 46-47 35.395240877363115 38.0 35.0 40.0 27.5 41.0 48-49 34.941584797946746 38.0 35.0 40.0 25.0 41.0 50-51 34.64732343255811 38.0 34.0 40.0 24.0 41.0 52-53 34.370090018469725 38.0 34.0 40.0 23.5 41.0 54-55 34.10020508585507 37.0 33.5 40.0 23.0 41.0 56-57 33.98358713493175 37.0 33.5 40.0 23.0 41.0 58-59 33.72161951005555 37.0 33.0 40.0 22.5 41.0 60-61 33.28870417264905 36.0 33.0 39.5 22.0 41.0 62-63 32.853755110013054 35.5 33.0 39.0 20.5 41.0 64-65 32.39474572437969 35.0 32.0 39.0 19.0 40.0 66-67 31.891700276531807 35.0 31.5 38.0 15.5 40.0 68-69 31.39316688882873 35.0 31.0 37.0 10.5 39.5 70-71 30.794864458295784 35.0 30.5 37.0 7.0 39.0 72-73 30.264582169695267 34.5 30.0 36.0 2.0 38.5 74-75 29.783956529337388 34.0 29.5 35.5 2.0 37.5 76-77 27.874325375476737 32.0 26.5 34.0 2.0 36.0 78-79 28.854185156375824 33.5 29.0 35.0 2.0 36.0 80-81 28.849535172995143 34.0 29.0 35.0 2.0 36.0 82-83 28.457393713441775 34.0 29.0 35.0 2.0 36.0 84-85 28.03948802209513 33.5 28.5 35.0 2.0 35.0 86-87 27.399600450949016 33.0 26.5 35.0 2.0 35.0 88-89 26.65725818887088 33.0 25.0 35.0 2.0 35.0 90-91 26.047756562918693 32.0 24.0 34.5 2.0 35.0 92-93 25.68773494066731 32.0 23.0 34.0 2.0 35.0 94-95 25.168696394121216 32.0 20.0 34.0 2.0 35.0 96-97 24.30195336995295 31.0 9.5 34.0 2.0 35.0 98-99 23.185807442029407 31.0 2.0 34.0 2.0 35.0 100-101 17.891176853568357 23.5 2.0 30.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0330638190958652 1101 2 0.027945212072644665 1101 3 -0.00794640781685274 1101 4 0.02641980321335069 1101 5 0.09249557662856489 1101 6 0.17477394657457523 1101 7 0.059855936476999716 1101 8 0.04623337761435664 1101 9 0.008418260325889548 1101 10-11 0.12298825785286027 1101 12-13 0.18621430562127728 1101 14-15 0.09798179006233454 1101 16-17 0.021780842482492346 1101 18-19 0.058233315304256905 1101 20-21 0.125069763812796 1101 22-23 0.03962601588624182 1101 24-25 0.11930874231156707 1101 26-27 0.08870838528802238 1101 28-29 0.17187369191569957 1101 30-31 -0.0056211397752221615 1101 32-33 0.10411374952787611 1101 34-35 0.1836466198707285 1101 36-37 0.31059650386898596 1101 38-39 0.4081921890249518 1101 40-41 0.33827718356258174 1101 42-43 0.24742001675388536 1101 44-45 0.528077369017609 1101 46-47 0.36178890770772654 1101 48-49 0.17346319241069352 1101 50-51 0.17621419269924843 1101 52-53 -0.03837769404881186 1101 54-55 0.08952738466548738 1101 56-57 -0.0823977755154246 1101 58-59 -0.13411442682660635 1101 60-61 -0.3523046914203647 1101 62-63 -0.14703398675268176 1101 64-65 -0.031466960145458245 1101 66-67 -0.21920433824368146 1101 68-69 -0.14546178113836206 1101 70-71 0.03583861088998219 1101 72-73 0.13571104541102486 1101 74-75 -0.02732469100865842 1101 76-77 0.06835943324372806 1101 78-79 0.3111587628709742 1101 80-81 0.4545639743964678 1101 82-83 0.07583160927763188 1101 84-85 0.005124482443239486 1101 86-87 0.22209698043664616 1101 88-89 0.036594203805922376 1101 90-91 0.0987069799310234 1101 92-93 -0.26768127224194416 1101 94-95 -0.2841284824266488 1101 96-97 -0.40860884691517185 1101 98-99 -0.10771871344363504 1101 100-101 0.20811949300365384 1102 1 0.10324546313975702 1102 2 0.18073104231383041 1102 3 0.16075431110708394 1102 4 0.003380900152123445 1102 5 0.09503934981965756 1102 6 0.16167822533153497 1102 7 0.27937051787774436 1102 8 0.1757194049798514 1102 9 0.15132251009397635 1102 10-11 0.25114442088625566 1102 12-13 0.07928705318621354 1102 14-15 0.3357448640419278 1102 16-17 0.43003288619770075 1102 18-19 0.4538335298218854 1102 20-21 0.3524711291884941 1102 22-23 0.3504356025049802 1102 24-25 0.4099112759386543 1102 26-27 0.48504238053384796 1102 28-29 0.5416593192348031 1102 30-31 0.557594522910307 1102 32-33 0.7348698516846426 1102 34-35 0.45292784088185556 1102 36-37 0.572076095705718 1102 38-39 0.5889287236956378 1102 40-41 0.5855866542258497 1102 42-43 0.8890596271434958 1102 44-45 0.8108166721252132 1102 46-47 0.7919366059915873 1102 48-49 0.8558955758754436 1102 50-51 0.4623270753523059 1102 52-53 0.5623513759882925 1102 54-55 0.636273746562523 1102 56-57 0.6428294247396806 1102 58-59 0.5160026157290503 1102 60-61 0.34874760449800135 1102 62-63 0.4304206456684696 1102 64-65 0.6278993431940663 1102 66-67 0.552473899812906 1102 68-69 0.5680593517466086 1102 70-71 0.7146365149948082 1102 72-73 0.4287975626883913 1102 74-75 0.5028146729755747 1102 76-77 0.1530177994311117 1102 78-79 0.49978932873368365 1102 80-81 0.641618270546747 1102 82-83 0.7350348502331059 1102 84-85 1.037385469224315 1102 86-87 1.114959558706591 1102 88-89 1.0749410411203932 1102 90-91 0.5968263271017094 1102 92-93 0.6467798246968286 1102 94-95 0.6050452623553575 1102 96-97 0.6882103495133975 1102 98-99 1.4480703635508014 1102 100-101 1.209956662530555 1103 1 0.05696222257080308 1103 2 0.14382318826312002 1103 3 0.22563990170695547 1103 4 0.09453736273716373 1103 5 0.20803873139046658 1103 6 0.13768317276505115 1103 7 0.1582078710008119 1103 8 0.33359200856673965 1103 9 0.18607823056398587 1103 10-11 0.24358721618619228 1103 12-13 0.12803164193080363 1103 14-15 0.15057479601471613 1103 16-17 0.254770053792015 1103 18-19 0.3647920950661572 1103 20-21 0.44340495726518014 1103 22-23 0.1701263879100452 1103 24-25 0.2998556173115716 1103 26-27 0.47277274540707026 1103 28-29 0.6436259240585542 1103 30-31 0.4193137560581093 1103 32-33 0.5268797465516855 1103 34-35 0.3602500424897741 1103 36-37 0.40881078958327777 1103 38-39 0.39675803723923764 1103 40-41 0.5059948174911568 1103 42-43 0.5034319215157907 1103 44-45 0.3508043035414232 1103 46-47 0.2615098898505792 1103 48-49 0.5273117787202182 1103 50-51 0.42491210936591983 1103 52-53 0.7261237940464227 1103 54-55 1.0428290959750086 1103 56-57 0.7503124179369607 1103 58-59 0.8373755285305364 1103 60-61 0.8480673323891565 1103 62-63 0.8153804477711262 1103 64-65 0.7759883969973984 1103 66-67 0.30429826592298426 1103 68-69 0.13684104624259064 1103 70-71 -0.10530164208621073 1103 72-73 0.13568686386340545 1103 74-75 0.20990187149133988 1103 76-77 -0.07388387032769828 1103 78-79 0.5377361438233557 1103 80-81 0.1880870398726593 1103 82-83 0.5343174723728694 1103 84-85 0.43274725030038397 1103 86-87 0.7353800905556938 1103 88-89 0.9084599032106837 1103 90-91 0.931322307311973 1103 92-93 1.1169839063294837 1103 94-95 1.117525163406686 1103 96-97 0.8958293971324451 1103 98-99 1.11202831036589 1103 100-101 0.9035249989560334 1104 1 0.06712212416982055 1104 2 0.10800171576268269 1104 3 -0.09849645394231743 1104 4 -0.14007869770915704 1104 5 0.05102590411934216 1104 6 -0.08057372408963204 1104 7 -0.07950782044181892 1104 8 -0.025736756149477458 1104 9 0.1273715006395406 1104 10-11 0.1615822961369915 1104 12-13 0.03628853878548455 1104 14-15 0.01209473978558151 1104 16-17 -0.002618805810861602 1104 18-19 0.13803757037805298 1104 20-21 0.10665921837921388 1104 22-23 0.0821154935153885 1104 24-25 0.05254934424883828 1104 26-27 0.09367768302787738 1104 28-29 0.09113251341016593 1104 30-31 0.14889555187607328 1104 32-33 0.1819684831533408 1104 34-35 0.4037011055718338 1104 36-37 0.4182994558985058 1104 38-39 0.42882972938473074 1104 40-41 0.22644816488177355 1104 42-43 0.05450027505646915 1104 44-45 0.13692625047517026 1104 46-47 0.0025220142575435034 1104 48-49 -0.2606674867867227 1104 50-51 -0.11260326578783975 1104 52-53 -0.06883491268349928 1104 54-55 0.3134845168149383 1104 56-57 0.16954453882959086 1104 58-59 0.4682084225737597 1104 60-61 0.464663712638675 1104 62-63 0.4477754574355117 1104 64-65 0.4410595619947628 1104 66-67 0.44261314907181415 1104 68-69 0.4026888761494618 1104 70-71 0.5286062827073259 1104 72-73 0.29894112497744274 1104 74-75 0.37805685304115855 1104 76-77 0.4836543479116244 1104 78-79 0.6450494455278033 1104 80-81 0.5610056833448063 1104 82-83 0.4125690977462675 1104 84-85 0.30225173105947434 1104 86-87 0.3331115964421798 1104 88-89 0.34895366529116245 1104 90-91 0.12399670548452946 1104 92-93 0.08726856631894009 1104 94-95 0.5403172913279626 1104 96-97 0.23025041277117353 1104 98-99 0.0704697090655877 1104 100-101 0.4478493707711806 1105 1 0.0174384130469889 1105 2 0.10763271207264324 1105 3 0.023973235040287477 1105 4 0.01179926749906457 1105 5 0.011133969485705109 1105 6 -0.07422158913971089 1105 7 0.12832691861985523 1105 8 0.15192534190006768 1105 9 0.03726870675446037 1105 10-11 0.09436102571000049 1105 12-13 0.12279354669270504 1105 14-15 0.023372415062340224 1105 16-17 -0.09582518430322295 1105 18-19 0.033958761732826304 1105 20-21 0.13084543345565436 1105 22-23 0.16649543552910018 1105 24-25 0.13074847445442828 1105 26-27 0.21301084064516118 1105 28-29 0.340233066915701 1105 30-31 0.13330185129620276 1105 32-33 0.04521307988502343 1105 34-35 0.19441670915644238 1105 36-37 0.29541793244040804 1105 38-39 0.23104375152495749 1105 40-41 0.24438767463401234 1105 42-43 0.3804557310395964 1105 44-45 0.2300304940176119 1105 46-47 0.37043846127915714 1105 48-49 0.38939511205354904 1105 50-51 0.42699544269925127 1105 52-53 0.4771356988083326 1105 54-55 0.5922338578797763 1105 56-57 0.6157886084131476 1105 58-59 0.5136255285305324 1105 60-61 0.5831863800082075 1105 62-63 0.6104994953901723 1105 64-65 0.38705982556882645 1105 66-67 0.429595884970599 1105 68-69 0.3182100938616408 1105 70-71 0.5248174055328363 1105 72-73 0.3106273400538804 1105 74-75 0.32900901434848606 1105 76-77 0.10379470110087041 1105 78-79 0.18749804858526176 1105 80-81 0.3740394208250386 1105 82-83 0.3583650914204881 1105 84-85 0.24971153601466867 1105 86-87 0.15008247150807463 1105 88-89 0.012626569877351557 1105 90-91 0.09608421207387963 1105 92-93 -0.15105180795622886 1105 94-95 0.007584687216208863 1105 96-97 0.016781778084826726 1105 98-99 0.264825929413508 1105 100-101 0.41007261800365313 1106 1 0.03886698447556114 1106 2 0.12370414064407242 1106 3 0.07575894932600136 1106 4 0.09751355321335353 1106 5 0.10934825519999691 1106 6 0.047206982288862775 1106 7 0.20154120433414136 1106 8 0.0733539133286456 1106 9 0.19441156389731873 1106 10-11 0.18275388285285743 1106 12-13 0.014757832406992577 1106 14-15 -0.0543061563662377 1106 16-17 0.1997105299824895 1106 18-19 0.1768159045899722 1106 20-21 0.2754882905985099 1106 22-23 0.21560257838623897 1106 24-25 0.2173556173115685 1106 26-27 0.13086798350230566 1106 28-29 0.2750544954871259 1106 30-31 0.48598042272477215 1106 32-33 0.30860593702787753 1106 34-35 -0.007369005129270079 1106 36-37 -0.37422492470243895 1106 38-39 -0.46717053418933574 1106 40-41 -0.5359694682231293 1106 42-43 -0.13561569753182567 1106 44-45 0.15413763687475068 1106 46-47 0.13561703270772796 1106 48-49 0.2117165406249768 1106 50-51 -0.04443312872932381 1106 52-53 0.00838569880833262 1106 54-55 0.059198143594059616 1106 56-57 0.13632432269886152 1106 58-59 0.040411242816254855 1106 60-61 0.05104352286534919 1106 62-63 0.039963781104461304 1106 64-65 0.42098839699739443 1106 66-67 0.2260244563991769 1106 68-69 0.43963866529020734 1106 70-71 0.39178169124712525 1106 72-73 0.2766987686253124 1106 74-75 0.5004375857770569 1106 76-77 0.3912947011008683 1106 78-79 0.3946409057281173 1106 80-81 0.3186822779678984 1106 82-83 0.5467579485633429 1106 84-85 0.5389972503003833 1106 86-87 0.8349039000795031 1106 88-89 0.6876265698773487 1106 90-91 1.0692984977881679 1106 92-93 0.8864481920437726 1106 94-95 0.7531204015019206 1106 96-97 0.7462460637991128 1106 98-99 0.6451830722706511 1106 100-101 1.3020369037179407 1107 1 0.09085111145969194 1107 2 -0.007645065705133902 1107 3 0.06236609218314726 1107 4 0.027721886546686392 1107 5 0.00438793773967916 1107 6 -0.027048970092089064 1107 7 0.05833683925477828 1107 8 0.010209072058799507 1107 9 0.12104846865922525 1107 10-11 -0.0773155615915826 1107 12-13 -0.09536121521205132 1107 14-15 -0.15440536271544403 1107 16-17 0.021238307760270914 1107 18-19 0.06669685697092831 1107 20-21 0.176827576312796 1107 22-23 0.06664424505291322 1107 24-25 0.0973903395337885 1107 26-27 7.09253343579519E-4 1107 28-29 0.16124001136014243 1107 30-31 0.056987367169220704 1107 32-33 0.16520315925009754 1107 34-35 0.10790877264850707 1107 36-37 0.09097844831342883 1107 38-39 -0.05003263736393393 1107 40-41 0.14480930161813887 1107 42-43 0.11634362786499963 1107 44-45 0.006592994017609044 1107 46-47 -0.14146630062560916 1107 48-49 -0.1936158006448636 1107 50-51 -0.385851779522973 1107 52-53 -0.19853394404881186 1107 54-55 -0.09938320561228409 1107 56-57 -0.24504968523764603 1107 58-59 -0.2993705032154921 1107 60-61 -0.12747830253147896 1107 62-63 0.2802663604695468 1107 64-65 -0.08702251570101538 1107 66-67 -0.18545867852146003 1107 68-69 -0.2767551839161406 1107 70-71 -0.09187406272112852 1107 72-73 0.24844976068880342 1107 74-75 0.46410326038023086 1107 76-77 0.23090283602150663 1107 78-79 0.3207073739820885 1107 80-81 0.23299279384091065 1107 82-83 0.15504167872207475 1107 84-85 0.7622859407765752 1107 86-87 0.39147632071442473 1107 88-89 0.5729440301948117 1107 90-91 0.7156774660421377 1107 92-93 0.5287596999802773 1107 94-95 0.6825598856289083 1107 96-97 0.7478581669737174 1107 98-99 0.3011354532230328 1107 100-101 0.5963573402258753 1108 1 0.13934583523374044 1108 2 0.12104053330169506 1108 3 0.14959840130791235 1108 4 0.12225750107182165 1108 5 0.1916939588445814 1108 6 0.1378427896844414 1108 7 0.21482931287364693 1108 8 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-1.0970737062369196 2316 52-53 -1.2499856284459767 2316 54-55 -0.9050939030118172 2316 56-57 -1.205108048121808 2316 58-59 -1.3095950895039081 2316 60-61 -0.8651038935480244 2316 62-63 -0.9369802411853065 2316 64-65 -0.8242750275213453 2316 66-67 -0.7304535071266613 2316 68-69 -0.9613555089752417 2316 70-71 -1.0104282884894502 2316 72-73 -0.5397336021953585 2316 74-75 -0.90697756113277 2316 76-77 -0.7488585410470527 2316 78-79 -0.8473297123063332 2316 80-81 -1.2365735477666533 2316 82-83 -0.9918628721053473 2316 84-85 -1.0285496088688184 2316 86-87 -1.1367779641555522 2316 88-89 -1.1626647168501059 2316 90-91 -1.3929735386859967 2316 92-93 -1.2722967421000995 2316 94-95 -1.3246024961677811 2316 96-97 -1.226480633262689 2316 98-99 -0.8859656106067568 2316 100-101 -0.25756922596797693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 0.0 4 25.0 5 96.0 6 198.0 7 279.0 8 458.0 9 710.0 10 1116.0 11 1537.0 12 2078.0 13 2652.0 14 3391.0 15 4199.0 16 5468.0 17 7297.0 18 7171.0 19 5100.0 20 4881.0 21 5322.0 22 5684.0 23 6098.0 24 6690.0 25 7782.0 26 8917.0 27 10237.0 28 12366.0 29 14443.0 30 17055.0 31 20759.0 32 25882.0 33 32420.0 34 47132.0 35 70848.0 36 83192.0 37 105933.0 38 54020.0 39 2218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.26821259827622 19.65472818057197 16.004382547553565 18.07267667359825 2 15.29885652214139 24.732120522634833 36.94954922231855 23.01947373290523 3 22.693083963554 26.950371621737386 32.27472252586275 18.081821888845866 4 12.266429998389468 18.463723618968643 37.70872668583314 31.56111969680875 5 11.384063955261471 40.34965681957585 33.85921207282347 14.407067152339211 6 26.82050104684593 37.120882434576416 17.374729721857662 18.68388679672 7 23.406172792971226 37.4332228805225 21.402259542403257 17.75834478410302 8 24.282713506882455 30.99948942702747 20.837202608376824 23.88059445771325 9 23.800924514013346 20.155467756802786 23.691099924956053 32.35250780422782 10-11 24.167149255214525 26.45453330546313 26.721384783554754 22.65693265576759 12-13 28.77849699652879 25.315424443766727 27.078957197536912 18.82712136216757 14-15 23.590134582929984 27.04063188013467 30.15283001087972 19.216403526055633 16-17 18.358988153778146 30.40781569650096 30.56707319162643 20.66612295809446 18-19 17.876757568047534 30.5499229424257 32.66512979853663 18.90818969099013 20-21 20.481686192941755 27.248234411247687 31.231388929818486 21.038690465992072 22-23 19.07726785206405 27.646755462959472 32.074691000551695 21.201285684424782 24-25 21.266820638113415 27.77662603785093 31.902843788657055 19.053709535378598 26-27 20.92057413808612 26.022110569983255 34.747433212114984 18.309882079815644 28-29 18.291191074224976 29.757666304582482 33.42325471423334 18.52788790695921 30-31 18.865414335107204 28.76307700742558 32.398082438688405 19.973426218778805 32-33 18.81658436961371 29.1066000980026 32.65850892132036 19.41830661106333 34-35 20.52945747658627 27.590032874754638 32.08325872183434 19.797250926824756 36-37 20.140165854644977 27.24665523038509 34.01211263309244 18.601066281877493 38-39 20.171128510880376 27.74716777773684 33.72732701446976 18.35437669691302 40-41 18.81613340152822 28.999788390249503 33.21759051028792 18.966487697934365 42-43 18.292029947132466 29.918861056305506 31.16425945614376 20.624849540418264 44-45 19.230153080150124 28.602832012761105 31.022696023377083 21.144318883711684 46-47 20.501345413642948 29.490815977740066 30.49167351295598 19.51616509566101 48-49 20.203584750162342 28.533391360070183 31.115466923903302 20.147556965864172 50-51 18.587350403920126 29.835005268523357 32.44725051614395 19.130393811412564 52-53 19.319875680621184 30.705652968005236 30.364442875793703 19.610028475579877 54-55 17.655030857111527 28.940663881930856 31.353549510158345 22.05075575079927 56-57 20.076226146133525 29.23604233986341 29.7216006634022 20.966130850600866 58-59 21.142216842327098 28.469154268492748 29.906220203904876 20.482408685275278 60-61 19.139137183948517 28.89195009997481 32.156080644636226 19.812832071440443 62-63 19.594893853245825 28.77180568132072 31.429479507821046 20.203820957612404 64-65 18.8726701716001 30.808262093862197 30.61276939142831 19.706298343109395 66-67 19.016863919265646 29.048432901990516 30.08476935795457 21.849933820789268 68-69 17.927250297698087 29.98740672840511 30.56644021622733 21.518902757669473 70-71 20.030411671228418 29.73541846031277 28.83797432569615 21.396195542762662 72-73 19.215448087750016 28.10644247144732 30.763906260172906 21.91420318062975 74-75 18.10615120498648 31.092866027708006 29.356917921111336 21.444064846194173 76-77 19.180801278588614 28.22669568184819 29.84105099435301 22.75145204521018 78-79 18.648246699175182 28.45074549124292 31.053882345684514 21.84712546389739 80-81 19.37129223902717 29.142862038095974 30.01614816908889 21.469697553787967 82-83 18.471519280126376 28.349564299641226 30.921532815450142 22.25738360478225 84-85 18.339569883630084 28.434728675795327 30.172310402017626 23.05339103855696 86-87 18.584127708213106 28.850706684239093 29.805842974873542 22.75932263267425 88-89 18.77277873949898 27.982788823231818 29.35002942522723 23.894403012041973 90-91 18.676969517150635 26.977128187928123 30.151575742721135 24.194326552200103 92-93 18.82095336652629 28.532462503726496 30.076174746169848 22.57040938357737 94-95 18.366852165331395 27.964694715344073 29.89135649325283 23.7770966260717 96-97 16.689825205856852 27.770029709179006 30.69905663933331 24.84108844563083 98-99 18.144358511319986 28.01657820161807 28.923102227674423 24.915961059387516 100-101 18.393921092421497 26.445560966834147 30.04047750607163 25.12004043467273 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13409.0 1 8493.0 2 3462.0 3 3098.5 4 2368.0 5 2330.0 6 3913.5 7 6571.0 8 8510.5 9 8515.5 10 6156.5 11 3699.0 12 3034.0 13 2785.0 14 2586.5 15 2346.5 16 2103.5 17 2034.5 18 1988.0 19 1968.0 20 2074.0 21 2127.0 22 2227.0 23 2469.5 24 2677.0 25 3013.0 26 3496.5 27 3924.0 28 4358.5 29 4775.0 30 5015.0 31 5452.0 32 5870.0 33 6126.0 34 6692.5 35 7020.0 36 7224.0 37 7606.0 38 8068.5 39 8376.5 40 8659.0 41 9214.0 42 9689.0 43 10273.5 44 11240.0 45 12263.0 46 15490.5 47 27273.5 48 52662.5 49 51801.5 50 29952.5 51 23306.0 52 19208.5 53 15894.5 54 13921.0 55 12869.5 56 12090.5 57 10878.0 58 9986.5 59 9381.0 60 8276.0 61 7054.5 62 5779.5 63 4278.0 64 2927.5 65 2107.0 66 1560.5 67 1179.5 68 856.0 69 588.5 70 435.0 71 324.5 72 255.5 73 178.0 74 166.0 75 159.0 76 96.5 77 43.0 78 26.5 79 17.0 80 7.0 81 9.0 82 10.5 83 5.5 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.387384392915029 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.1399963677359E-4 16-17 0.00205599854709436 18-19 5.996662429025217E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.566660612893168E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013620990374500135 36-37 0.02312998365481155 38-39 0.010536992553858596 40-41 0.006253662247412011 42-43 0.005054329761606969 44-45 0.0016276655164497017 46-47 0.0014563323041918385 48-49 0.0032553310328994035 50-51 5.1399963677359E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.996662429025217E-4 60-61 0.0018846653348364968 62-63 0.0014563323041918385 64-65 7.709994551603849E-4 66-67 0.0026556647899968816 68-69 0.0022273317593522234 70-71 8.566660612893168E-5 72-73 0.0 74-75 0.0 76-77 0.08823660431279962 78-79 0.0027413313961258136 80-81 8.566660612893168E-5 82-83 0.0 84-85 3.426664245157267E-4 86-87 0.09380493371118018 88-89 0.432445027738847 90-91 0.38815539237018937 92-93 0.0 94-95 0.00102799927354718 96-97 0.0 98-99 0.0 100-101 8.566660612893168E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 583658.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.62599703756184 #Duplication Level Percentage of deduplicated Percentage of total 1 68.90114082721816 24.546718389951053 2 14.657873904141482 10.44402744571799 3 6.184757538706172 6.61014461255953 4 3.1355233715757045 4.468245853878479 5 1.8886877155485835 3.364319147950664 6 1.184367282649128 2.531655918182581 7 0.8311013465687187 2.072616987753958 8 0.5890905844385448 1.6789551532850537 9 0.45814592710985474 1.4689714897788038 >10 2.0282214559134326 12.124207067929321 >50 0.07078579106228508 1.7222105161721988 >100 0.04237516743823749 3.194126666039812 >500 0.009149183878710368 2.424029743334365 >1k 0.01492761580210639 11.802605966854989 >5k 0.002407679968081676 5.761402328389336 >10k+ 0.0014446079808490056 5.785762712221854 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12072 2.0683345383769263 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11320 1.9394919627590128 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10334 1.7705574154727597 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8880 1.5214389248498263 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7670 1.3141257380178117 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6424 1.1006445555445141 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5338 0.9145766870324744 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5272 0.9032686950234556 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4711 0.8071507629467942 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4348 0.7449568068971898 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3474 0.5952115793838172 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3457 0.5922989147754336 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3097 0.5306189583626028 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3065 0.5251362955703511 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3035 0.5199962992026153 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2935 0.5028629779768289 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2933 0.5025203115523131 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2628 0.4502636818136649 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2457 0.4209657025175702 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2424 0.4153117065130607 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2239 0.383615062245356 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2218 0.3800170647879409 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1885 0.3229631051060724 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1832 0.31388244485640565 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1812 0.31045578061124834 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1807 0.30959911454995903 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1766 0.30257445284738665 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1718 0.2943504586590092 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1641 0.2811578013151538 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1613 0.27636047137193354 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1537 0.26333914724033597 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1384 0.23712516576488285 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1384 0.23712516576488285 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1354 0.23198516939714697 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1318 0.2258171737558639 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1281 0.21947784490232292 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1230 0.2107398510771719 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1178 0.20183052403976298 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1038 0.17784387432366214 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 997 0.17081921262108976 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 997 0.17081921262108976 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 974 0.1668785487391589 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 968 0.1658505494656117 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 904 0.15488522388110845 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 865 0.1482032286030518 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 797 0.13655257016951708 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 760 0.13021324131597614 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 745 0.12764324313210818 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 706 0.12096124785405153 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 678 0.11616391791083133 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 641 0.1098245890572904 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 635 0.10879658978374322 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 621 0.10639792481213313 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 603 0.1033139269914916 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 585 0.10022992917085005 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.4266642451572665E-4 0.0 16-17 0.0 0.0 0.0 7.70999455160385E-4 0.0 18-19 8.566660612893166E-5 0.0 0.0 0.0011136658796761117 0.0 20-21 1.7133321225786333E-4 0.0 0.0 0.0013706656980629066 0.0 22-23 1.7133321225786333E-4 0.0 0.0 0.0023986649716100867 0.0 24-25 1.7133321225786333E-4 0.0 0.0 0.00668199527805667 0.0 26-27 1.7133321225786333E-4 0.0 0.0 0.014049323405144795 0.0 28-29 1.7133321225786333E-4 0.0 0.0 0.03640830760479596 0.0 30-31 1.7133321225786333E-4 0.0 0.0 0.0878939378882839 0.0 32-33 1.7133321225786333E-4 0.0 0.0 0.17912887341559613 0.0 34-35 1.7133321225786333E-4 0.0 0.0 0.31491044412995284 0.0 36-37 1.7133321225786333E-4 0.0 0.0 0.4987509808826402 0.0 38-39 1.7133321225786333E-4 0.0 0.0 0.7421298088949351 0.0 40-41 1.7133321225786333E-4 0.0 0.0 0.9939896309139942 0.0 42-43 1.7133321225786333E-4 0.0 0.0 1.247477118449503 0.0 44-45 1.7133321225786333E-4 0.0 0.0 1.5337749161323926 0.0 46-47 1.7133321225786333E-4 0.0 0.0 1.8752420081623142 0.0 48-49 1.7133321225786333E-4 0.0 0.0 2.1982051132683864 0.0 50-51 1.7133321225786333E-4 0.0 0.0 2.5599751909508655 0.0 52-53 1.7133321225786333E-4 0.0 0.0 2.883109629269195 0.0 54-55 1.7133321225786333E-4 0.0 0.0 3.2348567140345885 0.0 56-57 1.7133321225786333E-4 0.0 0.0 3.687262061001477 0.0 58-59 1.7133321225786333E-4 0.0 0.0 4.146349403246422 0.0 60-61 1.7133321225786333E-4 0.0 0.0 4.700269678476094 0.0 62-63 1.7133321225786333E-4 0.0 0.0 5.2134126491883945 0.0 64-65 1.7133321225786333E-4 0.0 0.0 5.687491647505903 0.0 66-67 1.7133321225786333E-4 0.0 0.0 6.240640923280415 0.0 68-69 1.7133321225786333E-4 0.0 0.0 6.870804477964835 0.0 70-71 1.7133321225786333E-4 0.0 0.0 7.532750343523091 0.0 72-73 1.7133321225786333E-4 0.0 0.0 8.201035537934885 0.0 74-75 1.7133321225786333E-4 0.0 0.0 8.918836030689205 0.0 76-77 1.7133321225786333E-4 0.0 0.0 9.574271234181662 0.0 78-79 1.7133321225786333E-4 0.0 0.0 10.43076596225872 0.0 80-81 1.7133321225786333E-4 0.0 0.0 11.321355999575093 0.0 82-83 1.7133321225786333E-4 0.0 0.0 12.141870753078003 0.0 84-85 1.7133321225786333E-4 0.0 0.0 13.073666427942392 0.0 86-87 1.7133321225786333E-4 0.0 0.0 14.042213076836092 0.0 88-89 1.7133321225786333E-4 0.0 0.0 14.995511069838845 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGCGT 45 9.6627395E-4 42.185253 4 TCTAGAC 75 3.0924534E-4 31.638937 3 CTTAACC 95 3.5088484E-5 29.973732 2 ATTATAC 95 3.5088484E-5 29.973732 3 CTTCTAG 100 4.872019E-5 28.565588 1 CGCAGAA 305 0.0 28.00824 9 GTCCTAG 135 1.31756715E-5 24.686312 1 GTCGGGA 135 1.3503903E-5 24.608065 2 TTTGCGT 405 0.0 24.608063 3 GCCGATG 50 0.0016621143 23.729204 50-51 CTAAAGC 100 0.0016611221 23.729204 3 GTAAGCG 120 2.3283064E-10 23.729204 94-95 CGGGAGT 140 1.7896267E-5 23.729202 4 GAGTACT 4805 0.0 23.358822 12-13 ATGGGAT 840 0.0 23.164225 5 ACCTAGG 105 0.0021657993 22.671103 1 TATTAGC 105 0.0022061376 22.599243 2 GTACTTT 5070 0.0 21.950684 14-15 CTCCGAC 185 0.0 21.805216 94-95 CTTTGCG 460 0.0 21.665796 2 >>END_MODULE