##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938996_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 589527 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.858844463442725 33.0 31.0 34.0 30.0 34.0 2 31.867130767547543 33.0 31.0 34.0 30.0 34.0 3 31.887684533532816 34.0 31.0 34.0 30.0 34.0 4 35.41983318830181 37.0 35.0 37.0 33.0 37.0 5 35.48549091050961 37.0 35.0 37.0 33.0 37.0 6 35.61203982175541 37.0 35.0 37.0 33.0 37.0 7 35.56313620919822 37.0 35.0 37.0 33.0 37.0 8 35.56580445000823 37.0 35.0 37.0 33.0 37.0 9 37.12607395420396 39.0 37.0 39.0 33.0 39.0 10-11 37.04330336015144 39.0 37.0 39.0 33.0 39.0 12-13 36.93690195699264 39.0 37.0 39.0 33.0 39.0 14-15 38.01770402373428 40.0 38.0 41.0 33.0 41.0 16-17 37.96120957988353 40.0 38.0 41.0 33.0 41.0 18-19 37.91967713098806 40.0 37.0 41.0 33.0 41.0 20-21 37.806549148724315 40.0 37.0 41.0 33.0 41.0 22-23 37.82014564218433 40.0 37.0 41.0 33.0 41.0 24-25 37.70718643929794 40.0 37.0 41.0 33.0 41.0 26-27 37.52467486646074 40.0 36.5 41.0 32.5 41.0 28-29 37.362031764448446 40.0 36.0 41.0 32.0 41.0 30-31 37.13308381125886 39.0 35.5 41.0 32.0 41.0 32-33 36.83543332196829 39.0 35.0 41.0 31.5 41.0 34-35 36.443006851255326 39.0 35.0 41.0 30.5 41.0 36-37 36.10175700179975 38.5 35.0 41.0 30.0 41.0 38-39 35.829423419113965 38.0 35.0 40.0 30.0 41.0 40-41 35.67133396774024 38.0 35.0 40.0 30.0 41.0 42-43 35.44388976247059 38.0 35.0 40.0 29.0 41.0 44-45 35.23413940328433 38.0 35.0 40.5 26.5 41.0 46-47 34.8551949274588 38.0 35.0 41.0 24.0 41.0 48-49 34.56081824920657 38.0 35.0 40.0 23.0 41.0 50-51 33.28886717656698 36.5 32.5 39.0 21.5 40.5 52-53 33.469925889738725 37.5 33.5 39.5 22.0 40.5 54-55 33.9721929614759 38.0 34.0 40.0 22.0 41.0 56-57 33.84196482943105 37.5 34.0 40.0 20.5 41.0 58-59 33.64614852245953 37.0 34.0 40.0 20.0 41.0 60-61 33.292415784179525 36.5 33.5 40.0 18.5 41.0 62-63 32.93592999133203 36.0 33.0 39.5 16.5 41.0 64-65 32.471010657696766 35.5 33.0 39.0 11.5 41.0 66-67 32.00929473968113 35.0 32.0 39.0 7.5 40.0 68-69 31.606374262756415 35.0 32.0 38.0 3.5 40.0 70-71 31.08510212424537 35.0 31.5 37.0 2.0 39.0 72-73 30.596828474353167 35.0 31.0 37.0 2.0 39.0 74-75 30.152934471194705 35.0 31.0 36.0 2.0 38.5 76-77 29.7253272538832 35.0 30.0 35.5 2.0 37.5 78-79 29.280375623169082 34.0 29.5 35.0 2.0 37.0 80-81 28.829006135427214 34.0 29.0 35.0 2.0 36.5 82-83 28.471250680630405 34.0 29.0 35.0 2.0 36.0 84-85 28.121161541371304 34.0 29.0 35.0 2.0 36.0 86-87 27.741404549749205 34.0 27.5 35.0 2.0 35.0 88-89 27.239682830472564 34.0 26.5 35.0 2.0 35.0 90-91 26.732494864526984 33.5 25.0 35.0 2.0 35.0 92-93 26.29637149782792 33.0 24.5 35.0 2.0 35.0 94-95 25.779302729137086 33.0 21.5 35.0 2.0 35.0 96-97 25.110372383283547 33.0 16.5 35.0 2.0 35.0 98-99 24.42132760670843 33.0 2.0 35.0 2.0 35.0 100-101 22.06603938411642 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2611564899814063 1101 2 0.156516541829955 1101 3 0.2591087521976547 1101 4 0.15700126909659673 1101 5 0.08451082653658659 1101 6 0.193042400561815 1101 7 0.15853763721145953 1101 8 0.16672917727011338 1101 9 0.3253183640838557 1101 10-11 0.11021056212273805 1101 12-13 0.4120065989518409 1101 14-15 0.23392331605526095 1101 16-17 0.5554761543174749 1101 18-19 0.09895170365503958 1101 20-21 0.17073052775446484 1101 22-23 0.10634276020540057 1101 24-25 0.38036973310693156 1101 26-27 0.24157265925835247 1101 28-29 0.34024121489149906 1101 30-31 0.356221833206142 1101 32-33 0.45212582792272116 1101 34-35 0.47887392992374345 1101 36-37 0.3617625560550479 1101 38-39 0.5255024121586089 1101 40-41 0.5586099172520207 1101 42-43 0.16466291464888627 1101 44-45 0.5683829756714331 1101 46-47 0.5561409196458058 1101 48-49 0.6169774619157096 1101 50-51 0.8036546704674166 1101 52-53 0.7681329576140996 1101 54-55 0.7266467806635077 1101 56-57 0.5813307079154768 1101 58-59 0.39904172007235417 1101 60-61 0.47281711144454164 1101 62-63 0.5966304651289391 1101 64-65 0.6735521698313391 1101 66-67 0.6538634686720357 1101 68-69 0.4741309440407768 1101 70-71 0.1826016311788763 1101 72-73 0.06485322849942321 1101 74-75 0.12956068614921534 1101 76-77 0.11716821252176857 1101 78-79 0.16667591108596014 1101 80-81 0.2454633639787822 1101 82-83 0.3133021219782428 1101 84-85 0.09284202929711682 1101 86-87 -0.1264256828724406 1101 88-89 -0.11770083403367693 1101 90-91 -0.36091238076443233 1101 92-93 -0.2344398169788846 1101 94-95 -0.0479335841552988 1101 96-97 -0.3122942355264584 1101 98-99 -0.07487075040780766 1101 100-101 0.13327279908329004 1102 1 0.2257855660216741 1102 2 0.1833969321677209 1102 3 0.16946850892377086 1102 4 0.28113967407202267 1102 5 0.39240004757908764 1102 6 0.4429960135914044 1102 7 0.5423021975660589 1102 8 0.30798952843665717 1102 9 0.4847520994375998 1102 10-11 0.31802590761977 1102 12-13 0.4791749321133665 1102 14-15 0.5029247935661729 1102 16-17 0.40693820290353244 1102 18-19 0.7008346710467208 1102 20-21 0.6818719908338764 1102 22-23 0.6330513469490384 1102 24-25 0.6562158658080293 1102 26-27 0.49635910739084466 1102 28-29 0.6859753316492245 1102 30-31 0.7211515655361396 1102 32-33 0.6245238624896032 1102 34-35 0.5814836265185974 1102 36-37 0.5304376067371095 1102 38-39 0.5577430746332723 1102 40-41 0.630930640201548 1102 42-43 0.6748800743918721 1102 44-45 0.9339243287965431 1102 46-47 0.8690502417088197 1102 48-49 0.9990016518617608 1102 50-51 0.8377439396149242 1102 52-53 0.6972691291428106 1102 54-55 0.9320328233395188 1102 56-57 1.0558780054449386 1102 58-59 0.962875345471808 1102 60-61 0.8145705389262474 1102 62-63 1.128108319645662 1102 64-65 0.6016832903343783 1102 66-67 0.5269590258310899 1102 68-69 0.4828534125148245 1102 70-71 0.5938530485585254 1102 72-73 0.47181814620550355 1102 74-75 0.5520016716146294 1102 76-77 0.25626555643820126 1102 78-79 0.09646837234424765 1102 80-81 -0.020665557438253046 1102 82-83 0.06669867903421789 1102 84-85 0.0738079091033228 1102 86-87 0.3088370048229834 1102 88-89 0.46466352702875824 1102 90-91 0.5384811525482824 1102 92-93 0.5993178712319533 1102 94-95 0.5064834862498131 1102 96-97 0.38767483982659456 1102 98-99 0.5591480792661123 1102 100-101 0.559770067406145 1103 1 0.04147346664193563 1103 2 0.0794835957244544 1103 3 0.16213251486720281 1103 4 0.19415821274753853 1103 5 0.006716120766256495 1103 6 0.20508243526420245 1103 7 0.11548763615346047 1103 8 0.15012916457411762 1103 9 0.2318970051892535 1103 10-11 0.21939082408341903 1103 12-13 0.29253729151833596 1103 14-15 0.22693522913002084 1103 16-17 0.2741646580945343 1103 18-19 0.3590820491976885 1103 20-21 0.2883959459816836 1103 22-23 0.35276143178118247 1103 24-25 0.20300140114899534 1103 26-27 0.15467386749382683 1103 28-29 0.3142567463246664 1103 30-31 0.3383892510524831 1103 32-33 0.2825708225269281 1103 34-35 0.2809169270248262 1103 36-37 0.3778071189954417 1103 38-39 0.34792777029145583 1103 40-41 0.021331303654612555 1103 42-43 0.06464492865679716 1103 44-45 0.033601240975400515 1103 46-47 0.5671652747950873 1103 48-49 0.23962605795032488 1103 50-51 0.23282160163396526 1103 52-53 -0.09278771358080462 1103 54-55 0.012333104961520291 1103 56-57 0.2570908805809964 1103 58-59 0.2779378033580784 1103 60-61 0.15244956240626095 1103 62-63 0.053749743996462485 1103 64-65 0.1518102793977647 1103 66-67 0.30092540396477574 1103 68-69 0.33917781342016085 1103 70-71 0.3971374867830981 1103 72-73 0.3089231411086608 1103 74-75 0.2981634919639795 1103 76-77 0.48655287999251584 1103 78-79 0.336987386148909 1103 80-81 0.23846469705819473 1103 82-83 0.21617776471295969 1103 84-85 0.08301850361947061 1103 86-87 0.20123148295840565 1103 88-89 0.27509736313511723 1103 90-91 -0.052405006507679985 1103 92-93 -0.08415627310364471 1103 94-95 -0.05683136024027746 1103 96-97 -0.195353546980364 1103 98-99 -0.11966116070002286 1103 100-101 0.04256520899463112 1104 1 0.062025790809244086 1104 2 0.028740376427855097 1104 3 0.09056942635496057 1104 4 0.17247316538858826 1104 5 0.05220572820470437 1104 6 -0.04751483647122967 1104 7 0.12586629926212112 1104 8 0.14121678731475384 1104 9 0.3406036854763954 1104 10-11 0.10510739311352069 1104 12-13 0.2785537194792198 1104 14-15 0.37948628853892075 1104 16-17 0.16925920226032076 1104 18-19 0.3750687344882735 1104 20-21 0.35256158763809964 1104 22-23 0.16612965020912895 1104 24-25 0.1952286963033032 1104 26-27 0.07499582495069035 1104 28-29 0.2786680429800086 1104 30-31 0.3846675951918499 1104 32-33 0.23242697363080822 1104 34-35 -0.034397073572812076 1104 36-37 0.10059570281348584 1104 38-39 0.2861648221534949 1104 40-41 0.17875724232077772 1104 42-43 0.1000112568963516 1104 44-45 0.09836625303230306 1104 46-47 -0.23557584583645763 1104 48-49 0.1683235652643802 1104 50-51 0.26300207293020605 1104 52-53 0.04992324742710963 1104 54-55 0.003648314723790236 1104 56-57 0.08494127772903681 1104 58-59 -0.01704103626082798 1104 60-61 -0.18651785269161536 1104 62-63 -0.019816457767248608 1104 64-65 -0.05470148910974615 1104 66-67 -0.01324544960816354 1104 68-69 -0.18067785123818325 1104 70-71 -0.2361242695632768 1104 72-73 -0.3919788679114298 1104 74-75 -0.2740215519203595 1104 76-77 0.18534406281959548 1104 78-79 0.12363584585384757 1104 80-81 -0.04929042625574809 1104 82-83 -0.287199557195855 1104 84-85 -0.14256366745647853 1104 86-87 -0.229370111193127 1104 88-89 -0.081788620978962 1104 90-91 -0.14230188005268118 1104 92-93 -0.4388706253319157 1104 94-95 -0.9493494803367319 1104 96-97 -0.9652856530810823 1104 98-99 -0.5863900229039025 1104 100-101 1.685978232401908E-5 1105 1 -0.0015914884730889867 1105 2 -0.08909958289046926 1105 3 0.1965764934927705 1105 4 0.1332257764482705 1105 5 0.22048705766110288 1105 6 0.04193270111368719 1105 7 0.2666092614620652 1105 8 0.1909149344729002 1105 9 -0.0101258465539118 1105 10-11 0.1728189182853086 1105 12-13 0.2736217178623903 1105 14-15 0.1987298961693469 1105 16-17 0.06286422488297916 1105 18-19 0.035562521404941094 1105 20-21 0.18876893381050053 1105 22-23 0.24347699612075502 1105 24-25 0.010430214042862929 1105 26-27 0.1256034423342811 1105 28-29 0.2987383231881111 1105 30-31 0.20546284378037427 1105 32-33 0.0399224425784368 1105 34-35 0.21867687059418728 1105 36-37 0.3287064821544945 1105 38-39 0.24037185538990968 1105 40-41 0.21935090797475 1105 42-43 0.11490179449430826 1105 44-45 -0.06676941775903344 1105 46-47 -0.11248442940992476 1105 48-49 -0.1412940008759449 1105 50-51 -0.348527010558513 1105 52-53 -0.5279423987205121 1105 54-55 -0.2308809121939177 1105 56-57 -0.5824012437527131 1105 58-59 -0.3113247388151663 1105 60-61 -0.02707370524660746 1105 62-63 -0.2964029780494144 1105 64-65 -0.3208894071726789 1105 66-67 -0.6178540840337305 1105 68-69 -0.6842179396425969 1105 70-71 -0.6580304409218094 1105 72-73 -0.45089232807076485 1105 74-75 -0.5585423034882986 1105 76-77 -0.5589709333338959 1105 78-79 -0.391293808743054 1105 80-81 -0.4718974438453607 1105 82-83 -0.366711377049036 1105 84-85 -0.05443632858591485 1105 86-87 -0.278693387111403 1105 88-89 0.005722625158231409 1105 90-91 -0.11895555118303491 1105 92-93 0.0681997722271852 1105 94-95 0.2908125944288926 1105 96-97 0.18826160677313908 1105 98-99 -0.14019545495115793 1105 100-101 -0.326795887143728 1106 1 0.09530891094748029 1106 2 0.08866075264705131 1106 3 0.08542362875633103 1106 4 -0.04413453242080578 1106 5 0.08173235280199265 1106 6 0.06767381112708648 1106 7 0.12086924243049424 1106 8 0.1964122706589535 1106 9 0.21424365686753077 1106 10-11 0.1706672596898926 1106 12-13 0.1652781313202567 1106 14-15 0.03856770353831962 1106 16-17 0.2020992647947324 1106 18-19 0.20682393883276973 1106 20-21 0.08037231342974849 1106 22-23 0.18949460941617957 1106 24-25 0.2522660334005167 1106 26-27 0.1378103167319651 1106 28-29 0.2974109619700869 1106 30-31 0.38537332266079005 1106 32-33 0.3264263913265282 1106 34-35 0.26426172776648826 1106 36-37 0.13222368633174142 1106 38-39 -0.105249670893663 1106 40-41 0.28328244280434234 1106 42-43 0.3492643883641975 1106 44-45 0.4348700854805756 1106 46-47 -0.0289606793417434 1106 48-49 0.1603200461211287 1106 50-51 0.3056590260863601 1106 52-53 -0.039588629681361454 1106 54-55 -0.17373770870762684 1106 56-57 -0.03994083730137277 1106 58-59 0.03169055311339264 1106 60-61 0.046655452454814395 1106 62-63 -0.015311511345572626 1106 64-65 -0.12564608792109055 1106 66-67 0.18450698246162034 1106 68-69 0.17472871740726603 1106 70-71 0.06510110118099632 1106 72-73 -0.03946641482806612 1106 74-75 -0.06991124323579712 1106 76-77 -0.17472410475744482 1106 78-79 -0.2165912528150855 1106 80-81 -0.032639372095122354 1106 82-83 -0.04971751685229364 1106 84-85 -0.061189809120982375 1106 86-87 -0.265254612405208 1106 88-89 0.15518865016596095 1106 90-91 0.0867414952867307 1106 92-93 0.020432951408828615 1106 94-95 0.18163258848910147 1106 96-97 0.10898662480464338 1106 98-99 0.08316337503145022 1106 100-101 0.2402600984068215 1107 1 0.0751215511374781 1107 2 0.06349252464863753 1107 3 0.05735264313817723 1107 4 0.07148098413810544 1107 5 0.0942669879781377 1107 6 0.12081406709752684 1107 7 0.16883071492026858 1107 8 0.13756545709515677 1107 9 0.04446532199485631 1107 10-11 0.08308974286708803 1107 12-13 0.05457751118566989 1107 14-15 0.08532519133353844 1107 16-17 0.1611307992979647 1107 18-19 0.26130012302593286 1107 20-21 0.2769351845209158 1107 22-23 0.2591772852309333 1107 24-25 0.13295609805285125 1107 26-27 0.5267963307179855 1107 28-29 0.4322077689366992 1107 30-31 0.4971459839617509 1107 32-33 0.21744103705815832 1107 34-35 0.3057249814938885 1107 36-37 0.4924460111767033 1107 38-39 0.6237548151862953 1107 40-41 0.4555841976532662 1107 42-43 0.6359117033134041 1107 44-45 0.71950526162518 1107 46-47 0.5707391491316756 1107 48-49 0.38632281693521975 1107 50-51 0.5337069215870827 1107 52-53 0.46344607146654226 1107 54-55 0.31517903492609634 1107 56-57 0.4540062533438274 1107 58-59 0.5809384750028173 1107 60-61 0.44622993457646487 1107 62-63 0.37562066968094854 1107 64-65 0.33830034300647327 1107 66-67 0.25268880064343335 1107 68-69 0.31800289605880394 1107 70-71 0.5232750027511237 1107 72-73 0.6546884868740079 1107 74-75 0.874276644348317 1107 76-77 0.8470960562136582 1107 78-79 0.9408338692326836 1107 80-81 1.0949500196452107 1107 82-83 0.896795875415819 1107 84-85 1.1226899903248544 1107 86-87 0.9309948928065594 1107 88-89 0.8476942577659088 1107 90-91 0.7121868046943014 1107 92-93 0.8576806094112044 1107 94-95 1.0494086803218003 1107 96-97 1.1101246377351117 1107 98-99 1.156936300219467 1107 100-101 1.4618262680861989 1108 1 0.010671167315365437 1108 2 0.04789250512191501 1108 3 0.08358862356264041 1108 4 -0.07073761750457663 1108 5 0.13864146167198044 1108 6 0.11431227918212983 1108 7 -0.09595772526084545 1108 8 0.20789826564786296 1108 9 -0.1164135024965347 1108 10-11 0.07064450921767218 1108 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58-59 -1.242434827594856 2316 60-61 -1.2404092572566299 2316 62-63 -1.3762704447447973 2316 64-65 -1.7073576001583959 2316 66-67 -1.476976271126901 2316 68-69 -1.7998297560536294 2316 70-71 -1.5868745429939892 2316 72-73 -1.3661307216183296 2316 74-75 -1.5136899596847684 2316 76-77 -1.5118403674734608 2316 78-79 -1.343644464068369 2316 80-81 -1.2860865338290175 2316 82-83 -1.276157556194125 2316 84-85 -1.642770657061 2316 86-87 -1.6173580700200887 2316 88-89 -1.8325015851723165 2316 90-91 -2.0874470794271183 2316 92-93 -2.127563370985129 2316 94-95 -1.6727040407521407 2316 96-97 -2.179540337179173 2316 98-99 -2.4193596337634453 2316 100-101 -2.4481411882801503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2661.0 3 509.0 4 439.0 5 506.0 6 575.0 7 704.0 8 953.0 9 1113.0 10 1420.0 11 1944.0 12 2889.0 13 4315.0 14 4218.0 15 4419.0 16 5523.0 17 7257.0 18 6516.0 19 4432.0 20 4460.0 21 4757.0 22 5004.0 23 5723.0 24 6149.0 25 7020.0 26 8030.0 27 9404.0 28 11075.0 29 12662.0 30 15308.0 31 18494.0 32 22414.0 33 29302.0 34 40149.0 35 69962.0 36 78260.0 37 103149.0 38 77565.0 39 10246.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.123402315430646 19.034091875580476 15.564688151703912 18.277817657284963 2 14.978014702137413 24.21846085937898 37.47313379624128 23.330390642242328 3 22.592671821043304 26.91854522982476 32.10008590627727 18.38869704285467 4 12.104119265120387 17.849016877780418 38.41545828097939 31.63140557611981 5 11.552914208091012 39.83767033153627 33.996858683193956 14.612556777178758 6 26.821852554167087 36.50376788663915 18.10351120519746 18.570868353996303 7 23.398403411849795 37.250044991052725 21.841033877413814 17.510517719683673 8 23.856494324490427 30.566260682962604 21.340459269772605 24.23678572277436 9 23.89255523429051 20.24479151633896 24.278978795940855 31.583674453429676 10-11 23.937154517920927 26.15469108639259 26.997005256081163 22.911149139605318 12-13 28.648798235680612 25.18195817041845 27.374539268500143 18.7947043254008 14-15 23.74590798571021 26.678829514119997 30.505287357883155 19.069975142286637 16-17 18.385760233198337 30.17351511349659 30.79445491841199 20.64626973489308 18-19 17.874943650528856 30.491537179614525 32.90860052108935 18.72491864876727 20-21 20.45381224700481 27.258618816050422 31.310447173245677 20.977121763699095 22-23 18.969292752614127 27.49200796444022 32.238746407298756 21.2999528756469 24-25 21.208927732850764 27.45338144293128 31.93635470440026 19.401336119817696 26-27 20.81014588876311 25.76199045298635 34.868849577212174 18.559014081038367 28-29 18.247318593772157 29.607853626765916 33.5259207555405 18.61890702392143 30-31 18.784217962477413 28.634151394530562 32.406278444211985 20.175352198780043 32-33 18.895207659051312 29.011390767161714 32.5940145831706 19.499386990616372 34-35 20.601707585370754 27.48432556507998 32.022419294965445 19.891547554583813 36-37 20.1318867708815 27.121424902693192 33.96378702223254 18.782901304192766 38-39 20.356588988581166 27.612742413463064 33.619785135557045 18.410883462398722 40-41 18.86776364090622 28.88966958603818 33.32329985904816 18.91926691400744 42-43 18.176739314373055 29.815270347726404 31.112079671379444 20.895910666521097 44-45 19.11307522992134 28.577303052536386 31.06045352944188 21.2491681881004 46-47 20.30770569373252 29.398467442755155 30.633060619011182 19.660766244501144 48-49 20.028207420957788 28.540858952656585 31.194028831977285 20.236904794408346 50-51 18.673829712991854 29.672893807582284 32.59416428424565 19.059112195180212 52-53 19.212747296250413 30.471061603513768 30.534538715919446 19.781652384316377 54-55 17.64232663764995 28.773978457564336 31.772618619038763 21.811076285746946 56-57 20.01951317693795 29.05715024037539 29.91042530708948 21.012911275597183 58-59 21.097377101746893 28.337722455133736 30.09996622877786 20.46493421434151 60-61 19.32557798590536 28.378758485252455 32.49804809360968 19.797615435232508 62-63 19.751538384112376 28.445903641794917 31.791196024341183 20.01136194975152 64-65 18.959107172265615 30.350016255417405 31.12711907071917 19.56375750159781 66-67 19.046273902038127 28.68756624638792 30.488358557454497 21.777801294119453 68-69 18.024537950528106 29.48795633146033 31.278120668819597 21.20938504919197 70-71 20.178121565066597 29.271777051060603 29.543670164467173 21.006431219405624 72-73 19.330830275620787 27.77609011007765 31.4918508405154 21.40122877378616 74-75 18.436225650022482 30.78705817200989 30.14878989283407 20.627926285133555 76-77 19.500590371148938 28.051502204507294 30.39771563903411 22.050191785309657 78-79 19.07903210260688 28.312934952921236 31.68721857868964 20.92081436578224 80-81 19.812312043381624 29.043103580621555 30.586927857068535 20.557656518928287 82-83 19.005438899390597 28.151678415690235 31.602854569153184 21.240028115765984 84-85 19.07561297499644 28.268382425078464 30.926306504133706 21.72969809579139 86-87 19.44206781069238 28.60010253409344 30.61871051008579 21.339119145128393 88-89 19.65424338440171 27.918771765484742 30.065332712254534 22.361652137859018 90-91 19.588637837468855 26.92984402198027 31.01274787535411 22.468770265196763 92-93 19.989554994999366 28.313302362414365 30.94767939978769 20.74946324279858 94-95 19.562883684256338 27.903626877567493 30.687401758910177 21.846087679265988 96-97 18.3493197966771 27.528118170981347 31.356848409758616 22.76571362258294 98-99 19.822316810611497 27.996665133492794 29.717176354533514 22.463841701362192 100-101 20.158139185688984 26.3634129944409 30.95879986213157 22.51964795773855 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15790.0 1 9580.0 2 3450.0 3 3247.0 4 2468.5 5 2400.0 6 3899.5 7 6586.0 8 8662.0 9 8455.5 10 5905.5 11 3664.5 12 3187.0 13 2952.5 14 2708.0 15 2455.5 16 2395.0 17 2372.0 18 2305.0 19 2291.5 20 2290.0 21 2379.0 22 2526.5 23 2672.0 24 2885.5 25 3227.0 26 3658.0 27 4091.0 28 4510.5 29 4888.5 30 5164.5 31 5551.5 32 5883.5 33 6133.5 34 6620.0 35 6908.0 36 6999.5 37 7406.0 38 7934.5 39 8355.5 40 8524.0 41 9028.0 42 9665.0 43 10217.5 44 11040.5 45 12147.0 46 16214.5 47 28246.0 48 55815.0 49 56332.0 50 29732.5 51 18986.5 52 15781.5 53 14866.0 54 14022.5 55 12977.0 56 11949.0 57 10768.5 58 9989.5 59 9263.0 60 8075.0 61 6901.5 62 5716.5 63 4288.5 64 3074.5 65 2232.5 66 1610.5 67 1288.0 68 992.0 69 704.0 70 514.5 71 374.5 72 299.0 73 214.5 74 189.5 75 183.0 76 116.5 77 61.5 78 36.5 79 23.5 80 22.5 81 19.5 82 16.0 83 10.5 84 6.5 85 4.0 86 2.5 87 3.0 88 2.5 89 1.5 90 3.0 91 2.0 92 1.0 93 1.0 94 2.0 95 1.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2790372620762069 2 0.08430487492515186 3 0.08701891516419094 4 0.09957135126974677 5 0.10211576399384592 6 0.07972493202177339 7 0.0883759352837105 8 0.08566189504467141 9 0.09092034800780965 10-11 0.08396561989527197 12-13 0.08786705273889067 14-15 0.09753582109046745 16-17 0.08489857122744167 18-19 0.09643324224335781 20-21 0.09889284120998698 22-23 0.11186934610289266 24-25 0.12425215469350852 26-27 0.12832321505206717 28-29 0.11933295676025016 30-31 0.11110602228566291 32-33 0.10669570689722438 34-35 0.38420632133897176 36-37 0.5926785372001622 38-39 0.575037275646408 40-41 0.5354292509079313 42-43 0.5629937220856721 44-45 0.5864023191473843 46-47 0.5737650692843586 48-49 0.5955622049541411 50-51 0.5875048979944939 52-53 0.5918303996254625 54-55 0.5814831212141259 56-57 0.5523920024019257 58-59 0.5477272457410771 60-61 0.4954819711395746 62-63 0.5699484501982097 64-65 0.6053157870631879 66-67 0.6184619194710336 68-69 0.6206670771652528 70-71 0.6165960168066942 72-73 0.6067576209401775 74-75 0.604976532033308 76-77 0.5860630641175044 78-79 0.5646051834776016 80-81 0.5577352691225338 82-83 0.5731713729820687 84-85 0.5819071900014757 86-87 0.5734258142544786 88-89 0.5852149265428047 90-91 0.6016687954919792 92-93 0.610828681298736 94-95 0.6099805437240363 96-97 0.6042980219735483 98-99 0.6100653574815064 100-101 0.5870808292071441 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 589527.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.17526724854663 #Duplication Level Percentage of deduplicated Percentage of total 1 70.79588275875321 26.318558616534254 2 13.84066141417144 10.290605739369413 3 5.66357052032811 6.316342430225634 4 3.0144171287283945 4.482470494362986 5 1.750847085953102 3.2544104165822834 6 1.1285250214721574 2.5171931561939567 7 0.7715963597762624 2.0079010617881803 8 0.5816691511959703 1.7298964916756376 9 0.3955731709562911 1.323498451198963 >10 1.9317488338645783 11.897402260058957 >50 0.062296216982034885 1.5923367641562727 >100 0.03756095435588216 2.930267461990838 >500 0.007328966703586763 1.9926772338573218 >1k 0.014657933407173526 11.775901677505487 >5k 0.0022903020948708635 5.800424253707225 >10k+ 0.0013741812569225181 5.770113490792629 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11881 2.015344505001467 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11084 1.8801513755943324 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10920 1.8523324631441815 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9086 1.5412356007443255 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7234 1.2270854430755505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7203 1.2218269901124121 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5325 0.9032665170551984 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5215 0.8846074904118046 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4831 0.8194705246748665 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4469 0.758065364266607 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3819 0.6478074795556438 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3162 0.536362202240101 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2934 0.49768712883379385 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2824 0.4790281021904001 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2788 0.4729215116525622 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2773 0.470377098928463 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2772 0.47020747141352304 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2758 0.46783268620436386 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2453 0.4160962941476811 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2282 0.3870899890929508 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2070 0.3511289559256828 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2047 0.34722752308206406 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2025 0.34349571775338533 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1885 0.3197478656617933 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1876 0.3182212180273338 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1863 0.3160160603331145 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1859 0.31533755027335475 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1738 0.2948126209656216 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1625 0.275644711777408 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1614 0.2737788091130686 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1577 0.26750259106029073 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1476 0.25037021205135646 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1355 0.22984528274362326 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1327 0.22509571232530484 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1297 0.22000688687710654 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1202 0.20389227295781195 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1148 0.194732387151055 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1113 0.18879542412815697 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1100 0.18659026643393772 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1092 0.18523324631441818 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 977 0.1657260820963247 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 935 0.15860172646884707 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 898 0.15232550841606915 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 897 0.15215588090112922 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 825 0.1399426998254533 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 805 0.13655014952665442 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 769 0.13044355898881646 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 736 0.12484585099579833 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 714 0.12111404566711957 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 683 0.11585559270398132 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 642 0.10890086459144366 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 630 0.10686533441216432 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 624 0.10584756932252466 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.240687873498585E-4 0.0 12-13 0.0 0.0 0.0 6.785100597597735E-4 0.0 14-15 0.0 0.0 0.0 8.481375746997169E-4 0.0 16-17 0.0 0.0 0.0 0.0012722063620495753 0.0 18-19 0.0 0.0 0.0 0.0016962751493994337 0.0 20-21 0.0 0.0 0.0 0.0020355301792793204 0.0 22-23 0.0 0.0 0.0 0.0039014328436186973 0.0 24-25 0.0 0.0 0.0 0.007633238172297451 0.0 26-27 0.0 0.0 0.0 0.014248711254955243 0.0 28-29 0.0 0.0 0.0 0.04028653479823655 0.0 30-31 0.0 0.0 0.0 0.09694212478817764 0.0 32-33 0.0 0.0 0.0 0.1891346791580369 0.0 34-35 0.0 0.0 0.0 0.33611692085349776 0.0 36-37 0.0 0.0 0.0 0.5322911418815424 0.0 38-39 0.0 0.0 0.0 0.7874957381086871 0.0 40-41 0.0 0.0 0.0 1.0559312805011476 0.0 42-43 0.0 0.0 0.0 1.3065559338249138 0.0 44-45 0.0 0.0 0.0 1.608492910418013 0.0 46-47 0.0 0.0 0.0 1.9764149903227501 0.0 48-49 0.0 0.0 0.0 2.3274591324909633 0.0 50-51 0.0 0.0 0.0 2.69639897748534 0.0 52-53 0.0 0.0 0.0 3.016231656904603 0.0 54-55 0.0 0.0 0.0 3.3673606128302858 0.0 56-57 0.0 0.0 0.0 3.8066110627672693 0.0 58-59 0.0 0.0 0.0 4.260195037716678 0.0 60-61 0.0 0.0 0.0 4.82140767089548 0.0 62-63 0.0 0.0 0.0 5.340467866611707 0.0 64-65 0.0 0.0 0.0 5.824669608007776 0.0 66-67 0.0 0.0 0.0 6.397501725959964 0.0 68-69 0.0 0.0 0.0 7.036912643526081 0.0 70-71 0.0 0.0 0.0 7.711690897957176 0.0 72-73 0.0 0.0 0.0 8.379853679305613 0.0 74-75 0.0 0.0 0.0 9.10679239458074 0.0 76-77 0.0 0.0 0.0 9.760706464674222 0.0 78-79 0.0 0.0 0.0 10.625721977110462 0.0 80-81 0.0 0.0 0.0 11.521779324780713 0.0 82-83 0.0 0.0 0.0 12.361096268703555 0.0 84-85 0.0 0.0 0.0 13.318643590539534 0.0 86-87 0.0 0.0 0.0 14.289676299813241 0.0 88-89 0.0 0.0 0.0 15.24773250419404 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATCGG 55 0.002626156 34.416973 5 TATATGG 75 3.1446115E-4 31.548891 2 GTCGGGA 205 1.8189894E-12 30.009924 2 GCTACCC 85 6.5444765E-4 27.837255 3 GGTCGGG 240 0.0 27.664036 1 ATGGGAT 785 0.0 27.128027 5 TTTGCGT 335 0.0 26.840101 3 CGGGAGT 230 5.456968E-12 26.747974 4 ATACTAT 120 1.7541774E-4 23.659658 6 TCGGGAG 265 3.274181E-11 23.215221 3 GTACATG 3785 0.0 22.803642 1 CTTTGCG 395 0.0 22.763124 2 GAGTACT 4725 0.0 22.735233 12-13 TACATGG 3740 0.0 22.649405 2 CTATACT 105 0.0022430029 22.534922 4 AGTACTT 4705 0.0 22.479841 12-13 TTGCGTA 65 2.9107882E-4 21.957191 60-61 TTAGAAC 110 0.0029385644 21.510609 3 ACATGGG 3785 0.0 21.379894 3 GTACTTT 5050 0.0 21.0378 14-15 >>END_MODULE