##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938996_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 589527 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04882388762516 33.0 31.0 34.0 30.0 34.0 2 32.238509856206754 34.0 31.0 34.0 30.0 34.0 3 32.29639863823031 34.0 31.0 34.0 30.0 34.0 4 35.890027089514135 37.0 35.0 37.0 35.0 37.0 5 35.82304796896495 37.0 35.0 37.0 35.0 37.0 6 35.916105623661004 37.0 35.0 37.0 35.0 37.0 7 35.86350413127813 37.0 35.0 37.0 35.0 37.0 8 35.87046734076641 37.0 35.0 37.0 35.0 37.0 9 37.47378661197875 39.0 37.0 39.0 34.0 39.0 10-11 37.4092670903962 39.0 37.0 39.0 34.0 39.0 12-13 37.348893265278775 39.0 37.0 39.0 34.0 39.0 14-15 38.41654411078712 40.0 38.0 41.0 33.5 41.0 16-17 38.41537961789706 40.0 38.0 41.0 33.5 41.0 18-19 38.50141299719945 40.0 38.0 41.0 34.0 41.0 20-21 38.48435355802194 40.0 38.0 41.0 34.0 41.0 22-23 38.39308377733335 40.0 38.0 41.0 34.0 41.0 24-25 38.26697759390156 40.0 37.0 41.0 33.5 41.0 26-27 37.99363472750188 40.0 37.0 41.0 33.0 41.0 28-29 37.76266311805905 40.0 36.0 41.0 33.0 41.0 30-31 37.53525453456754 39.5 36.0 41.0 33.0 41.0 32-33 37.21510465169534 39.0 35.0 41.0 33.0 41.0 34-35 37.05106297082237 39.0 35.0 41.0 32.0 41.0 36-37 36.845027454213295 39.0 35.0 41.0 31.5 41.0 38-39 36.636245668137335 39.0 35.0 41.0 31.0 41.0 40-41 36.462798141560945 39.0 35.0 41.0 31.0 41.0 42-43 36.24573683648077 39.0 35.0 41.0 30.5 41.0 44-45 35.942929670736035 39.0 35.0 41.0 30.0 41.0 46-47 35.49305120885049 38.0 35.0 41.0 27.5 41.0 48-49 35.10053653182975 38.0 35.0 40.0 26.0 41.0 50-51 34.79182717670268 38.0 34.5 40.0 25.0 41.0 52-53 34.5490104778916 38.0 34.0 40.0 24.0 41.0 54-55 34.33220531035899 38.0 34.0 40.0 24.0 41.0 56-57 34.015038327336995 37.0 33.5 40.0 23.0 41.0 58-59 33.71262893811479 37.0 33.0 40.0 22.5 41.0 60-61 33.1931887767651 36.0 33.0 39.5 21.5 41.0 62-63 32.87988506039588 36.0 33.0 39.0 20.0 41.0 64-65 32.60962262966751 35.0 33.0 39.0 20.0 40.0 66-67 32.10191221097592 35.0 32.0 38.5 16.5 40.0 68-69 31.67023138889313 35.0 32.0 37.5 13.5 39.5 70-71 31.139105588039225 35.0 31.0 37.0 9.0 39.0 72-73 30.604245437443918 35.0 31.0 36.0 6.0 39.0 74-75 30.18808468484056 34.5 30.0 36.0 2.0 38.0 76-77 28.21410130494447 32.5 27.0 34.5 2.0 36.0 78-79 29.192579813986466 34.0 29.0 35.0 2.0 36.0 80-81 29.195772203817636 34.0 29.0 35.0 2.0 36.0 82-83 28.89013141043582 34.0 29.0 35.0 2.0 36.0 84-85 28.528981709065064 34.0 29.0 35.0 2.0 35.5 86-87 27.999369833781998 34.0 28.0 35.0 2.0 35.0 88-89 27.403415789268344 33.0 26.5 35.0 2.0 35.0 90-91 26.89261221284182 33.0 25.5 35.0 2.0 35.0 92-93 26.564448278026283 33.0 25.0 35.0 2.0 35.0 94-95 26.170588454812076 33.0 24.5 35.0 2.0 35.0 96-97 25.534736322509403 32.5 21.5 35.0 2.0 35.0 98-99 24.717086749207414 32.0 10.0 34.0 2.0 35.0 100-101 20.67283771566018 27.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2762216888451263 1101 2 0.12743641207143241 1101 3 0.10216697008027609 1101 4 0.10092973386507964 1101 5 0.1819583496002295 1101 6 0.08937002687848405 1101 7 0.18503135568063556 1101 8 0.24567862773838556 1101 9 0.27719899960010963 1101 10-11 0.18286189624802063 1101 12-13 0.31645481743522197 1101 14-15 0.37134967508085026 1101 16-17 0.19541319352984488 1101 18-19 0.40398195684276317 1101 20-21 0.4126467396716933 1101 22-23 0.32014531570788307 1101 24-25 0.3557926415730748 1101 26-27 0.2418667390821696 1101 28-29 0.5368273377317507 1101 30-31 0.4847723555890795 1101 32-33 0.2627153656431389 1101 34-35 0.5786506674424317 1101 36-37 0.5104036749033725 1101 38-39 0.5231555828701531 1101 40-41 0.45826256294440526 1101 42-43 0.581146412437775 1101 44-45 0.45106433451568506 1101 46-47 0.41526499748328405 1101 48-49 0.3649092342008373 1101 50-51 0.4869396874398575 1101 52-53 0.5330313740937811 1101 54-55 0.7482858916323494 1101 56-57 0.3758111634460093 1101 58-59 0.6145557252407414 1101 60-61 0.7073225211563425 1101 62-63 0.7517969075103608 1101 64-65 0.6988457514532129 1101 66-67 0.5899119596747866 1101 68-69 0.2192160412211983 1101 70-71 0.34147479842569695 1101 72-73 0.31941270982361303 1101 74-75 0.12976118769752887 1101 76-77 0.23710637771041476 1101 78-79 0.18776817727974304 1101 80-81 0.23026296074236186 1101 82-83 0.3342760580301416 1101 84-85 -0.00241560480550973 1101 86-87 -0.024895644687536134 1101 88-89 -0.28101761795008784 1101 90-91 -0.10183208092108487 1101 92-93 -0.06002181592845446 1101 94-95 -0.20192112620457792 1101 96-97 -0.44185872043758323 1101 98-99 -0.8026015025350581 1101 100-101 -0.11346970237533327 1102 1 0.19392433118440522 1102 2 0.19469064703002914 1102 3 0.20360324812721586 1102 4 0.23652056642529828 1102 5 0.2859407569121899 1102 6 0.3896449126290946 1102 7 0.2460937891067374 1102 8 0.3619587363182575 1102 9 0.3771474585218684 1102 10-11 0.3311661947739495 1102 12-13 0.4271650534933613 1102 14-15 0.3317455105617242 1102 16-17 0.04159056356042612 1102 18-19 0.36946833281774616 1102 20-21 0.4629525500692395 1102 22-23 0.40978384094582765 1102 24-25 0.4913199068034615 1102 26-27 0.5610812530498066 1102 28-29 0.4317453874173509 1102 30-31 0.26536542159602305 1102 32-33 0.4154728133289396 1102 34-35 0.3468085893061499 1102 36-37 0.1242201199434092 1102 38-39 0.2426999597385091 1102 40-41 0.3562473067852423 1102 42-43 0.15873806965525006 1102 44-45 0.3157277712747728 1102 46-47 0.518886617247567 1102 48-49 0.6728637406091167 1102 50-51 0.5993645233674201 1102 52-53 0.5634698168660037 1102 54-55 0.5426232451697857 1102 56-57 0.23764671304569873 1102 58-59 0.19988369830193875 1102 60-61 0.20862651043579206 1102 62-63 0.19969574955413094 1102 64-65 0.3768531733684739 1102 66-67 0.5300092005090633 1102 68-69 0.6771361869106443 1102 70-71 0.6502522440498595 1102 72-73 0.5057354256948265 1102 74-75 0.49597429287568673 1102 76-77 0.31735755456503156 1102 78-79 0.3657481106199505 1102 80-81 0.6514480619346799 1102 82-83 0.49148321880394263 1102 84-85 0.5711214875904638 1102 86-87 0.3907384823784028 1102 88-89 0.53633110898528 1102 90-91 0.5350129178762906 1102 92-93 0.47966481431584995 1102 94-95 0.5349565152756846 1102 96-97 0.48952636013830286 1102 98-99 0.7432889554933233 1102 100-101 0.935195383698268 1103 1 0.10494214420812398 1103 2 0.12590231274644026 1103 3 0.10647302818561144 1103 4 0.008703914444438965 1103 5 -0.03256163601097484 1103 6 0.055651581714599274 1103 7 -0.0010390224484027044 1103 8 0.16930164134426207 1103 9 -0.04233801980360141 1103 10-11 0.09871919327081713 1103 12-13 -0.07885612862796876 1103 14-15 0.2441887110316756 1103 16-17 0.07865236490460603 1103 18-19 0.2284862840608568 1103 20-21 0.200311523099181 1103 22-23 -0.09374939217844513 1103 24-25 0.03554147248359385 1103 26-27 -0.0013990938808632336 1103 28-29 -0.06293143837438464 1103 30-31 -0.18630616913636544 1103 32-33 -0.0733158665031155 1103 34-35 -0.31964383730799284 1103 36-37 -0.4486940630936189 1103 38-39 -0.3551109906925589 1103 40-41 -0.48262171306651425 1103 42-43 -0.33571069414509935 1103 44-45 -0.1086385792150395 1103 46-47 -0.06802918986728201 1103 48-49 -0.1924449205633323 1103 50-51 -0.4198082335910627 1103 52-53 -0.40692186232679717 1103 54-55 -0.35918570108015047 1103 56-57 -0.38820204038818673 1103 58-59 -0.47472885547404076 1103 60-61 -0.5199728088337139 1103 62-63 -0.4656854767973968 1103 64-65 -0.08535937349653011 1103 66-67 -0.24206184425673882 1103 68-69 -0.03437057720068992 1103 70-71 0.02802656634291978 1103 72-73 0.1778894020938644 1103 74-75 0.3351612173215166 1103 76-77 -0.009719623686148537 1103 78-79 0.14582378573527777 1103 80-81 0.11698824058323964 1103 82-83 0.23779708048089532 1103 84-85 0.22190674649265674 1103 86-87 0.10880375648472906 1103 88-89 0.48813746362167976 1103 90-91 0.27751365216679247 1103 92-93 0.3299991324623299 1103 94-95 0.11278612532423438 1103 96-97 -0.032222460677534315 1103 98-99 0.20115968581001908 1103 100-101 0.7255874084395373 1104 1 0.12529641554744586 1104 2 0.07363373921324268 1104 3 0.027571561204844386 1104 4 0.14507197304028097 1104 5 0.24978178064353074 1104 6 0.07229496848745498 1104 7 0.07889787861552122 1104 8 0.16415921703865877 1104 9 0.11077418928459082 1104 10-11 0.032762389634982014 1104 12-13 0.06170752277126468 1104 14-15 0.20786860206786884 1104 16-17 0.13206756007351572 1104 18-19 0.15952046728404667 1104 20-21 0.0042826560308526496 1104 22-23 -0.07086039210199857 1104 24-25 -0.128877120854888 1104 26-27 -0.043561761270247246 1104 28-29 -0.2671239040278621 1104 30-31 -0.030761732336067382 1104 32-33 0.02160876918392063 1104 34-35 0.02357997628607933 1104 36-37 -0.39596336629512763 1104 38-39 0.1150229903082689 1104 40-41 -0.002453055447737995 1104 42-43 -0.01767954773193736 1104 44-45 0.06276050765718821 1104 46-47 0.06995977476812953 1104 48-49 -0.2441679801668073 1104 50-51 -0.25607066401197187 1104 52-53 -0.03343766138308979 1104 54-55 0.29597305389723516 1104 56-57 0.31402032081847864 1104 58-59 0.36934102590273454 1104 60-61 0.3115971594083362 1104 62-63 0.22619123010526465 1104 64-65 0.05973343336373915 1104 66-67 -0.16640740127163056 1104 68-69 -0.2864814033936973 1104 70-71 -0.4457157981993696 1104 72-73 -0.6595596079894541 1104 74-75 -0.50648686017621 1104 76-77 -0.5098082262670864 1104 78-79 -0.4391200298045703 1104 80-81 -0.2090414603917381 1104 82-83 -0.34017492805275396 1104 84-85 -0.24006711053404572 1104 86-87 -0.5848311035926876 1104 88-89 -0.3061534030158022 1104 90-91 -0.37487062591327813 1104 92-93 -0.06605440434422505 1104 94-95 -0.2550431461888216 1104 96-97 -0.42502897348842694 1104 98-99 -0.10701572645257684 1104 100-101 0.693839887574498 1105 1 0.060310041302081174 1105 2 0.11393076762522725 1105 3 0.11149056293903215 1105 4 0.12160177367397296 1105 5 0.1820311384727944 1105 6 0.16530867247607972 1105 7 0.054117259784604244 1105 8 0.15604243975781174 1105 9 0.11322552121840346 1105 10-11 0.0796538921189125 1105 12-13 0.17459592197327112 1105 14-15 0.36087800318662033 1105 16-17 0.3282164915274919 1105 18-19 0.13014360107722212 1105 20-21 0.15057371258099295 1105 22-23 0.059190590386620556 1105 24-25 0.12034379891615288 1105 26-27 0.017822589322378235 1105 28-29 -0.010816287948166803 1105 30-31 0.06926369371324625 1105 32-33 -0.026540996766037495 1105 34-35 0.017001800963683422 1105 36-37 -0.25462061011138104 1105 38-39 -0.17177632644814622 1105 40-41 -0.34444319796400436 1105 42-43 -0.4122364173289057 1105 44-45 -0.24918777967020844 1105 46-47 0.04314181224221869 1105 48-49 -0.12003670351835183 1105 50-51 -0.3290434255417054 1105 52-53 -0.3584966628564956 1105 54-55 -0.10438943029298287 1105 56-57 -0.29815093340290133 1105 58-59 -0.40156701003274975 1105 60-61 -0.4082827514048972 1105 62-63 -0.30653013951125985 1105 64-65 -0.5477894174831448 1105 66-67 -0.543834285081104 1105 68-69 -0.47045190917649293 1105 70-71 -0.6181174515445242 1105 72-73 -0.544389383849051 1105 74-75 -0.2569627836633579 1105 76-77 -0.5205099852281379 1105 78-79 -0.535895485191638 1105 80-81 -0.43389248982075657 1105 82-83 -0.3233383197573403 1105 84-85 -0.6574128917657127 1105 86-87 -0.46118552968111715 1105 88-89 -0.41086634943855316 1105 90-91 -0.13354156067916279 1105 92-93 -0.3172279143125394 1105 94-95 -0.3585793228933447 1105 96-97 -0.24046845297155883 1105 98-99 -0.07021640969616172 1105 100-101 -0.06886335783251596 1106 1 0.01008245037177602 1106 2 -0.12532760717552094 1106 3 -0.16200645807449376 1106 4 -0.1554318467492024 1106 5 -0.0486903035627293 1106 6 9.310473010728515E-4 1106 7 -0.09320468289036654 1106 8 0.1114606797616915 1106 9 -0.08641809138607215 1106 10-11 -0.10912839033628075 1106 12-13 0.013479120247097853 1106 14-15 -0.04282921365814474 1106 16-17 0.07420107231772732 1106 18-19 0.0962186121385642 1106 20-21 0.17951352068941873 1106 22-23 0.17127533543023077 1106 24-25 0.23350285800493964 1106 26-27 0.012898018593418215 1106 28-29 0.20184940491621006 1106 30-31 0.512097761438973 1106 32-33 0.26683007645702617 1106 34-35 0.2274355268484456 1106 36-37 0.203522053945008 1106 38-39 0.3808559702504155 1106 40-41 0.26064562613942144 1106 42-43 0.295957765313581 1106 44-45 0.21830853826613605 1106 46-47 0.20619207619920843 1106 48-49 -0.02743052977344007 1106 50-51 0.2534772273503023 1106 52-53 0.09600636461805578 1106 54-55 0.24388530317410329 1106 56-57 0.19419855656310148 1106 58-59 0.23496481936193447 1106 60-61 0.24705790766933688 1106 62-63 0.2407472547392686 1106 64-65 -0.11377108898851418 1106 66-67 0.08941247682422926 1106 68-69 0.1772321788536182 1106 70-71 -0.09156719667904767 1106 72-73 0.2506848332199674 1106 74-75 0.2233556011986586 1106 76-77 0.21318741278021136 1106 78-79 0.4218168471456707 1106 80-81 0.49429666218331647 1106 82-83 0.4971042552022027 1106 84-85 0.26708510507043925 1106 86-87 0.31402675719861506 1106 88-89 0.37098577403634536 1106 90-91 0.8171521219299827 1106 92-93 0.6755670598978618 1106 94-95 0.6517850044254452 1106 96-97 0.645719414098366 1106 98-99 0.542245633227914 1106 100-101 0.5360860630379669 1107 1 0.1323281292212286 1107 2 0.24104658467037154 1107 3 0.23276857820997776 1107 4 -0.08850349208500319 1107 5 -0.012339861551915021 1107 6 0.05578656913772306 1107 7 0.030327445358743432 1107 8 0.03130498639845314 1107 9 0.2431768419824465 1107 10-11 0.054267176361172176 1107 12-13 0.20723819136465949 1107 14-15 0.13379369173834732 1107 16-17 0.34518009329674726 1107 18-19 0.2193384168621364 1107 20-21 0.21014091216586195 1107 22-23 0.14594217802159903 1107 24-25 0.28995819512065424 1107 26-27 0.1859914346113669 1107 28-29 0.10584399523155241 1107 30-31 0.018002234324576705 1107 32-33 0.21139730168462734 1107 34-35 0.12252392901231701 1107 36-37 0.3216939763622122 1107 38-39 0.04656054869084869 1107 40-41 0.17572347282104062 1107 42-43 0.07580998856202825 1107 44-45 0.10851502988771955 1107 46-47 -0.024181323985509096 1107 48-49 0.0964314572960916 1107 50-51 0.14798837657843222 1107 52-53 0.36541855620006203 1107 54-55 0.28153868752558964 1107 56-57 0.5443766803261312 1107 58-59 0.47765844648952793 1107 60-61 0.23434185014195918 1107 62-63 0.15905771786105305 1107 64-65 0.3286190680242811 1107 66-67 0.29070882198850256 1107 68-69 0.19466521751307297 1107 70-71 0.06044824071374677 1107 72-73 0.25627594022616407 1107 74-75 0.2687111890070817 1107 76-77 -0.07002937043656843 1107 78-79 0.060934804659858344 1107 80-81 0.12779052680925673 1107 82-83 0.4378616110538971 1107 84-85 0.24503404292503816 1107 86-87 0.450753238264717 1107 88-89 0.4806308459455657 1107 90-91 0.7909778245292678 1107 92-93 0.8223542349869248 1107 94-95 0.3498487331495532 1107 96-97 -0.007500007988980428 1107 98-99 0.3862718899900166 1107 100-101 0.8142724992432733 1108 1 0.002823253643050805 1108 2 -0.119352614734062 1108 3 -0.19054632678032135 1108 4 -0.09866043810581715 1108 5 -0.08360629145708742 1108 6 -0.2740533117492774 1108 7 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sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 14.0 5 69.0 6 209.0 7 309.0 8 422.0 9 692.0 10 1097.0 11 1505.0 12 2056.0 13 2760.0 14 3332.0 15 4265.0 16 5280.0 17 7307.0 18 7198.0 19 4902.0 20 4525.0 21 4950.0 22 5181.0 23 5674.0 24 6325.0 25 7185.0 26 8308.0 27 9511.0 28 11433.0 29 13710.0 30 16331.0 31 19564.0 32 23857.0 33 30501.0 34 43144.0 35 69176.0 36 81893.0 37 112911.0 38 69600.0 39 4322.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.14522647039289 19.787400354559807 16.035060889941708 18.032312285105593 2 15.187090667603009 24.7476366646481 36.89076157665382 23.174511091095066 3 22.64747840217666 26.956695791710985 32.34050348838984 18.055322317722514 4 12.22217133396774 18.33368106973896 37.76264700344513 31.681500592848167 5 11.32521945634197 40.37402993935796 33.90797676095161 14.392773843348458 6 26.957035046740863 36.963531780563066 17.473160686448626 18.60627248624745 7 23.415382162309783 37.250711163356385 21.528445686117852 17.805460988215977 8 24.332218880560177 31.084242112744626 20.81685826094479 23.766680745750406 9 23.68899134390791 20.30746683358014 23.706971860491546 32.2965699620204 10-11 24.163439503194933 26.48691238908481 26.656624717782222 22.693023389938034 12-13 28.708269510980838 25.392051593904945 27.060083762066878 18.83959513304734 14-15 23.60725675125526 27.104763197177366 30.035622201112773 19.252357850454608 16-17 18.38850580561987 30.44248237958322 30.52585600027141 20.6431558145255 18-19 17.93760082235419 30.542197388754033 32.60172986139735 18.918471927494416 20-21 20.470054806650076 27.304432197337864 31.21502492676332 21.010488069248737 22-23 19.11116878446619 27.699325052118056 32.050779692872425 21.138726470543332 24-25 21.29512303931796 27.773791531176688 31.804734982452032 19.126350447053316 26-27 20.85120783272013 26.014245318704653 34.71545832506399 18.41908852351122 28-29 18.294157858757952 29.818311968747828 33.3283293216426 18.559200850851614 30-31 18.90685244272103 28.744739426692924 32.26391666539446 20.084491465191586 32-33 18.90888797290031 29.107318239877056 32.611653071021344 19.372140716201294 34-35 20.503466847607132 27.60136553508195 32.100337142978304 19.794830474332606 36-37 20.139275293479 27.189472077084886 33.945935400692136 18.72531722874398 38-39 20.150838851310656 27.802801656495944 33.63268678416862 18.413672708024777 40-41 18.888928469649475 28.95632319613511 33.1637015022595 18.991046831955924 42-43 18.22364710422418 29.92294611316786 31.215602457921456 20.637804324686503 44-45 19.215385267800347 28.558246045545143 31.068063271277722 21.158305415376784 46-47 20.45996392007457 29.517517931824717 30.48143037312275 19.54108777497797 48-49 20.13875577795683 28.578177346168527 31.061871845977695 20.22119502989695 50-51 18.612690779401973 29.776302007132895 32.488327417528595 19.12267979593654 52-53 19.320658765417022 30.64617905541222 30.297933767240515 19.73522841193024 54-55 17.639989347392063 28.985949752937522 31.39338825872267 21.980672640947745 56-57 20.010957937465122 29.27567354845495 29.71382141954482 20.99954709453511 58-59 21.183901290105347 28.482567760003597 29.843916844988044 20.489614104903012 60-61 19.201987398104713 28.883004574102927 32.064520889647326 19.85048713814503 62-63 19.601731557672508 28.869670091446192 31.27702378347387 20.25157456740743 64-65 18.75532207230894 30.827987230419012 30.58753982012668 19.82915087714537 66-67 18.982008045617278 29.009004882839832 30.022722125956403 21.986264945586488 68-69 17.919028079109367 29.96464878637112 30.592200680901016 21.524122453618496 70-71 20.019184871939707 29.65792915337211 28.81623742424011 21.50664855044807 72-73 19.152897153141417 28.01406890608912 30.8484598669781 21.98457407379136 74-75 18.10290283566317 31.16252521088941 29.350140027513582 21.384431925933843 76-77 19.196044164327166 28.25810418120893 29.780520073844986 22.76533158061892 78-79 18.682297303257194 28.456251293411995 30.97912560260277 21.88232580072804 80-81 19.364507478028997 29.153117668910838 29.9621561014169 21.520218751643267 82-83 18.446313739659082 28.425415629818477 30.923350414824085 22.204920215698348 84-85 18.260995679587193 28.49352107706687 30.181908547021596 23.06357469632434 86-87 18.606586966215787 28.732677892680442 29.816405835262138 22.844329305841633 88-89 18.789271208156144 27.976519054628724 29.283117299094975 23.95109243812016 90-91 18.701826559017437 26.9241079748879 30.136394079916528 24.237671386178132 92-93 18.87648911754678 28.51251935874014 30.01457100353334 22.596420520179738 94-95 18.434948696158106 27.920264890327328 29.856393345851846 23.78839306766272 96-97 16.688152855005548 27.821504055800762 30.66845228200283 24.82189080719086 98-99 18.145733783185502 27.995918761990545 29.007492447334897 24.850855007489056 100-101 18.359473066406117 26.644377497777867 30.017268168895583 24.978881266920432 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13993.0 1 8651.5 2 3350.5 3 3089.0 4 2340.0 5 2308.0 6 3954.5 7 6691.0 8 8626.5 9 8435.0 10 5971.5 11 3673.0 12 3073.5 13 2852.0 14 2640.5 15 2359.0 16 2128.5 17 2049.0 18 2003.5 19 1982.5 20 2056.5 21 2122.5 22 2279.0 23 2468.5 24 2664.0 25 3052.0 26 3499.0 27 3956.0 28 4458.5 29 4857.0 30 5024.5 31 5466.5 32 5924.0 33 6171.0 34 6759.0 35 6990.5 36 7167.0 37 7592.0 38 8129.5 39 8531.0 40 8714.0 41 9197.0 42 9788.0 43 10456.0 44 11221.0 45 12359.5 46 15773.5 47 27562.0 48 53500.0 49 52847.5 50 30551.0 51 23741.0 52 19544.0 53 16151.0 54 14068.0 55 12787.5 56 12037.0 57 11012.5 58 10179.5 59 9530.0 60 8328.0 61 6983.0 62 5686.5 63 4210.0 64 2962.0 65 2129.0 66 1501.5 67 1155.5 68 857.0 69 614.5 70 446.0 71 311.5 72 262.5 73 203.0 74 156.5 75 141.5 76 96.5 77 48.5 78 30.0 79 16.5 80 8.0 81 5.5 82 4.0 83 2.5 84 2.0 85 1.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3935358346606686 2 0.0 3 0.0 4 0.0 5 3.3925502987988676E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011873926045796036 16-17 0.002035530179279321 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002459598966629179 36-37 0.007972493202177338 38-39 0.015266476344594904 40-41 0.0025444127240991507 42-43 7.633238172297452E-4 44-45 3.3925502987988676E-4 46-47 4.240687873498584E-4 48-49 3.3925502987988676E-4 50-51 7.633238172297452E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.544412724099151E-4 60-61 0.002120343936749292 62-63 0.0 64-65 1.6962751493994338E-4 66-67 0.002289971451689236 68-69 0.0023747852091592072 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12314957584639889 78-79 1.6962751493994338E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14664298666558104 88-89 0.4381478710898738 90-91 0.3395094711522967 92-93 0.0 94-95 0.0 96-97 1.6962751493994338E-4 98-99 0.0 100-101 5.088825448198302E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 589527.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.59775843468578 #Duplication Level Percentage of deduplicated Percentage of total 1 68.88365499502413 24.52103710611105 2 14.543681202159842 10.35444900371133 3 6.172266714017616 6.591565784401525 4 3.1667589045588076 4.509180740214984 5 1.945609743861252 3.462967283507186 6 1.2488069809745217 2.6672837544168155 7 0.8118882727858348 2.023098182740909 8 0.5954132158182105 1.6956300660412866 9 0.43405920532294173 1.3906381263693979 >10 2.0513930793676973 12.107441020820612 >50 0.07872041761615402 1.923546343367632 >100 0.04007584896767101 3.1061044564259435 >500 0.007633495041461145 1.9490215288940964 >1k 0.016221176963104933 12.262905149337595 >5k 0.0023854672004566075 5.689288270862961 >10k+ 0.0014312803202739645 5.74584318277667 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12050 2.0440115550263176 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11377 1.929852237471736 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10405 1.7649742929501107 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8751 1.4844103832394446 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7509 1.273733009684035 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6526 1.1069891624980706 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5373 0.9114086377723157 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5340 0.9058109297792976 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4574 0.775876253335301 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4457 0.7560298340873277 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3462 0.587250456722084 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3390 0.575037275646408 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3321 0.563332977115552 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3047 0.5168550380220075 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3024 0.5129536051783887 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2955 0.5012493066475326 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2908 0.4932768134453553 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2647 0.4490040320460301 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2478 0.4203369820211797 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2477 0.42016735450623977 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2244 0.3806441435252329 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2117 0.35910144912786013 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1877 0.31839084554227376 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1854 0.31448941269865505 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1852 0.31415015766877513 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1809 0.30685617452635755 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1752 0.2971874061747808 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1746 0.2961696410851411 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1736 0.2944733659357417 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1544 0.26190488306727255 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1520 0.2578338227087139 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1389 0.23561261825158136 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1350 0.22899714516892355 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1340 0.22730087001952412 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1290 0.21881949427252695 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1261 0.2139002963392686 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1259 0.21356104130938872 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1234 0.20932035343589014 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1112 0.18862579661321702 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1097 0.1860813838891179 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1042 0.176751870567421 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1040 0.17641261553754112 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 950 0.1611461391929462 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 892 0.1513077433264295 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 875 0.14842407557245046 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 862 0.14621891787823119 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 817 0.13858567970593375 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 801 0.13587163946689465 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 693 0.11755186785338076 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 680 0.1153467101591615 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 674 0.11432894506952183 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 646 0.10957937465120342 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 619 0.10499943174782495 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 617 0.10466017671794507 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 613 0.10398166665818528 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 596 0.10109799890420626 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.240687873498585E-4 0.0 12-13 0.0 0.0 0.0 6.785100597597735E-4 0.0 14-15 0.0 0.0 0.0 8.481375746997169E-4 0.0 16-17 0.0 0.0 0.0 0.0012722063620495753 0.0 18-19 0.0 0.0 0.0 0.0016962751493994337 0.0 20-21 0.0 0.0 0.0 0.0020355301792793204 0.0 22-23 0.0 0.0 0.0 0.0039014328436186973 0.0 24-25 0.0 0.0 0.0 0.007718051929767423 0.0 26-27 0.0 0.0 0.0 0.014418338769895187 0.0 28-29 0.0 0.0 0.0 0.04062578982811644 0.0 30-31 0.0 0.0 0.0 0.09728137981805753 0.0 32-33 0.0 0.0 0.0 0.19108539557984622 0.0 34-35 0.0 0.0 0.0 0.3396790986672366 0.0 36-37 0.0 0.0 0.0 0.5380584773895004 0.0 38-39 0.0 0.0 0.0 0.7975885752476137 0.0 40-41 0.0 0.0 0.0 1.0718762669055022 0.0 42-43 0.0 0.0 0.0 1.3262327255579471 0.0 44-45 0.0 0.0 0.0 1.6309685561475555 0.0 46-47 0.0 0.0 0.0 2.0020287450786816 0.0 48-49 0.0 0.0 0.0 2.3561261825158137 0.0 50-51 0.0 0.0 0.0 2.729052274111279 0.0 52-53 0.0 0.0 0.0 3.0515989937695815 0.0 54-55 0.0 0.0 0.0 3.4057812449641833 0.0 56-57 0.0 0.0 0.0 3.8491027552597252 0.0 58-59 0.0 0.0 0.0 4.308199624444682 0.0 60-61 0.0 0.0 0.0 4.8733136904671035 0.0 62-63 0.0 0.0 0.0 5.396020877754538 0.0 64-65 0.0 0.0 0.0 5.882851845632176 0.0 66-67 0.0 0.0 0.0 6.458906886368224 0.0 68-69 0.0 0.0 0.0 7.104339580714709 0.0 70-71 0.0 0.0 0.0 7.784630729381352 0.0 72-73 0.0 0.0 0.0 8.455677178483768 0.0 74-75 0.0 0.0 0.0 9.188976925569143 0.0 76-77 0.0 0.0 0.0 9.849930537532632 0.0 78-79 0.0 0.0 0.0 10.721052640506713 0.0 80-81 0.0 0.0 0.0 11.620672165990701 0.0 82-83 0.0 0.0 0.0 12.46075243373077 0.0 84-85 0.0 0.0 0.0 13.41914789314145 0.0 86-87 0.0 0.0 0.0 14.390689484959976 0.0 88-89 0.0 0.0 0.0 15.348660875583306 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGGGC 60 0.003969505 31.633842 4 GTAAGCG 130 0.0 29.20047 94-95 CGCAGAA 255 0.0 27.912216 9 AGCCGAT 35 0.008434213 27.114723 52-53 TTTGCGT 460 0.0 26.819996 3 TCGTGGT 90 8.996662E-4 26.361536 9 AGGTGCG 90 8.996662E-4 26.361536 9 CTTTGCG 505 0.0 24.430096 2 CCAGTCG 100 0.0016626996 23.725382 8 TAAGATA 105 0.0022082308 22.595602 4 AGACGGC 130 6.330083E-10 21.900352 94-95 GGTGCGA 65 2.9622708E-4 21.900352 10-11 GTACATG 3820 0.0 21.713146 1 GAGTACT 4565 0.0 21.6205 12-13 GCTCTAA 200 5.1961433E-7 21.451092 1 TACATGG 3725 0.0 21.145844 2 GCTTTGC 650 0.0 20.53438 1 ATGGGAG 630 0.0 20.33604 5 ACATGGG 3740 0.0 20.172918 3 CAGTCGG 165 6.366623E-5 20.130629 9 >>END_MODULE