FastQCFastQC Report
Fri 27 May 2016
SRR938995_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938995_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3943649
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104730.2655662306660659No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89360.22659217389782915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72400.18358631815356793No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT59230.1501908511634783No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46330.1174800292825249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45060.11425966154695816No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41080.10416748549376478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG100800.022.1332472
GTACATG109350.021.3996621
GAGTACT101800.019.88241612-13
ACATGGG105400.019.7291643
AGAGTAC146350.019.32900210-11
GTATCAA232200.019.3173871
AGTACTT106400.018.5556112-13
GTACTTT109200.018.3621114-15
GGTATCA187350.017.6079941
CAACGCA255200.017.3360825
GTGTAGC24400.017.1187321
TCAACGC258300.017.1096824
TATCAAC259750.017.1053522
ATCAACG258900.017.0700323
AACGCAG260250.016.954896
CATGGGG71800.016.8888574
GAGTACA89200.016.602311
AGTACAT80300.016.3398842
ACGCAGA281300.015.4673387
CGCAGAG280750.015.2952758
Produced by FastQC (version 0.11.5)