FastQCFastQC Report
Fri 27 May 2016
SRR938995_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938995_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3943649
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT114090.28930059444945533No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90290.22895039594040953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77870.19745672092014274No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53190.1348750864998381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.1191028917634404No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42480.10771749717076748No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT6500.026.282933
TACATGG104900.020.4026092
GTACATG111350.020.2791581
GAGTACT107700.019.98334712-13
AGAGTAC160150.019.55796210-11
ACATGGG107550.018.9291673
GTACTTT112750.018.9199414-15
GTATCAA246850.018.4688821
AGTACTT114900.018.46265412-13
CGCCGTT1300.00760750518.2520357
GGTATCA199900.017.2062471
CATGGGG73750.016.9230384
CTTTGCG10700.016.853282
CAACGCA272350.016.5357345
ATCAACG273900.016.390183
TCAACGC275550.016.3437024
TATCAAC276900.016.2126052
AACGCAG278250.016.2021666
GAGTACA94650.016.1061271
GGACCGA8600.016.0023656
Produced by FastQC (version 0.11.5)