##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938995_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3943649 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2023374798315 33.0 31.0 34.0 30.0 34.0 2 32.44256017713544 34.0 31.0 34.0 31.0 34.0 3 32.5388836582566 34.0 31.0 34.0 31.0 34.0 4 36.08047546827824 37.0 35.0 37.0 35.0 37.0 5 36.00148187630288 37.0 35.0 37.0 35.0 37.0 6 36.039135075155016 37.0 35.0 37.0 35.0 37.0 7 35.975802613265024 37.0 35.0 37.0 35.0 37.0 8 35.94429321676447 37.0 35.0 37.0 35.0 37.0 9 37.63405237129369 39.0 37.0 39.0 35.0 39.0 10-11 37.543739693872354 39.0 37.0 39.0 35.0 39.0 12-13 37.45685696166164 39.0 37.0 39.0 34.0 39.0 14-15 38.793252898521146 40.0 38.0 41.0 34.5 41.0 16-17 38.8802577257763 40.0 38.0 41.0 35.0 41.0 18-19 38.90497556958036 40.0 38.0 41.0 35.0 41.0 20-21 38.867886949371 40.0 38.0 41.0 34.5 41.0 22-23 38.79355528344434 40.0 38.0 41.0 34.0 41.0 24-25 38.72580939632305 40.0 38.0 41.0 34.0 41.0 26-27 38.571531847788684 40.0 38.0 41.0 34.0 41.0 28-29 38.419005088941745 40.0 38.0 41.0 34.0 41.0 30-31 38.242641015972765 40.0 38.0 41.0 34.0 41.0 32-33 38.16748511340639 40.0 38.0 41.0 33.0 41.0 34-35 38.081334063959545 40.0 38.0 41.0 33.0 41.0 36-37 37.96671268157993 40.0 37.5 41.0 33.0 41.0 38-39 37.8303981921312 40.0 37.0 41.0 33.0 41.0 40-41 37.67448827215607 40.0 37.0 41.0 33.0 41.0 42-43 37.503077226193305 40.0 37.0 41.0 32.0 41.0 44-45 37.276728354881485 40.0 37.0 41.0 31.5 41.0 46-47 37.00742561013924 40.0 36.0 41.0 31.0 41.0 48-49 36.70475313599157 39.5 36.0 41.0 30.5 41.0 50-51 36.45402468627405 39.0 35.5 41.0 30.0 41.0 52-53 36.19869402677571 39.0 35.0 40.0 29.5 41.0 54-55 35.89473885733746 39.0 35.0 40.0 29.0 41.0 56-57 35.748502465609896 38.5 35.0 40.0 28.5 41.0 58-59 35.45952733115955 38.0 34.5 40.0 28.0 41.0 60-61 35.08789486589704 38.0 34.0 40.0 27.5 41.0 62-63 34.66985385869787 37.0 34.0 40.0 26.5 41.0 64-65 34.25440917789591 37.0 33.5 39.5 26.0 41.0 66-67 33.7912073310784 36.0 33.0 39.0 26.0 40.5 68-69 33.29655567724207 35.5 32.5 39.0 25.0 40.0 70-71 32.76518752049181 35.0 32.0 38.0 23.5 40.0 72-73 32.24349365270591 35.0 31.0 37.0 22.5 39.5 74-75 31.714951558822808 35.0 31.0 37.0 21.5 39.0 76-77 29.808141140349967 32.5 28.5 35.0 18.5 37.0 78-79 30.779625798340575 34.0 30.0 36.0 20.0 38.0 80-81 30.762208426764147 34.0 31.0 35.5 19.5 37.0 82-83 30.3435526336142 34.0 30.0 35.0 17.5 37.0 84-85 29.88021918279238 34.0 30.0 35.0 12.5 36.0 86-87 29.18163584030932 34.0 29.0 35.0 6.0 36.0 88-89 28.476500063773422 33.0 28.5 35.0 2.0 35.0 90-91 27.88844354555895 33.0 27.0 35.0 2.0 35.0 92-93 27.460303389069363 33.0 26.0 34.5 2.0 35.0 94-95 26.88626485267832 32.0 25.5 34.0 2.0 35.0 96-97 26.012750500868613 32.0 24.5 34.0 2.0 35.0 98-99 24.753615877072225 32.0 11.0 34.0 2.0 35.0 100-101 19.079847876928195 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13745432368734356 1101 2 0.11130031991516631 1101 3 0.17247078075373423 1101 4 0.1229468646544305 1101 5 0.1230166123291383 1101 6 0.10612975496039923 1101 7 0.12424981180065231 1101 8 0.09402117672983934 1101 9 0.12917049653957235 1101 10-11 0.24069018470089532 1101 12-13 0.19332826055167374 1101 14-15 0.18274393470807127 1101 16-17 0.18263852727329777 1101 18-19 0.21322479389286997 1101 20-21 0.27956320325766626 1101 22-23 0.2301564680950392 1101 24-25 0.1930031787271247 1101 26-27 0.14467790714451212 1101 28-29 0.15277050760229116 1101 30-31 0.1179335143197946 1101 32-33 0.12994695751579854 1101 34-35 0.24304333902876607 1101 36-37 0.22669569819584012 1101 38-39 0.21964350201016458 1101 40-41 0.23533050164831337 1101 42-43 0.3007924113858209 1101 44-45 0.22716166730484844 1101 46-47 0.20613000304283702 1101 48-49 0.20437073669924644 1101 50-51 0.15192205754163268 1101 52-53 0.24846043469661083 1101 54-55 0.15925175001264336 1101 56-57 0.22912850047988087 1101 58-59 0.2368223092788213 1101 60-61 0.22797667982873548 1101 62-63 0.3369984442214573 1101 64-65 0.37756644037922626 1101 66-67 0.1590372228803929 1101 68-69 0.2901795040050601 1101 70-71 0.29225537344670016 1101 72-73 0.22292503165042632 1101 74-75 0.25335406072715827 1101 76-77 0.12554309589974366 1101 78-79 0.5206215400365863 1101 80-81 0.5069230053891651 1101 82-83 0.4227406607768529 1101 84-85 0.452037858124374 1101 86-87 0.6070948454076195 1101 88-89 0.5419326606761494 1101 90-91 0.656011692056552 1101 92-93 0.5796651977237346 1101 94-95 0.5719810574031712 1101 96-97 0.6646520753484317 1101 98-99 0.8707689303923303 1101 100-101 1.0418078395711063 1102 1 0.1106438727092467 1102 2 0.13389960238402665 1102 3 0.13055865577388914 1102 4 0.11950887904721696 1102 5 0.17119680040629248 1102 6 0.17832482692094942 1102 7 0.18679690254806758 1102 8 0.16826196491125955 1102 9 0.21939604973183435 1102 10-11 0.23469160277805656 1102 12-13 0.2857100382526667 1102 14-15 0.3200105703503979 1102 16-17 0.34633592452178164 1102 18-19 0.2872307238291114 1102 20-21 0.3096251711739484 1102 22-23 0.25745996759167866 1102 24-25 0.2828959316716322 1102 26-27 0.340943627286066 1102 28-29 0.27430509185743546 1102 30-31 0.30755830904858783 1102 32-33 0.31102262703434747 1102 34-35 0.34048148983573867 1102 36-37 0.33472587708647694 1102 38-39 0.2812953105762048 1102 40-41 0.31339901783709934 1102 42-43 0.35033039596522286 1102 44-45 0.3106954297624469 1102 46-47 0.3771582710328616 1102 48-49 0.43660900517166823 1102 50-51 0.34241145020893526 1102 52-53 0.3719325900966197 1102 54-55 0.38468404335520745 1102 56-57 0.47591796463621705 1102 58-59 0.25680761685309506 1102 60-61 0.3541965754167862 1102 62-63 0.4901848753041591 1102 64-65 0.4476304579129078 1102 66-67 0.35963017149133947 1102 68-69 0.3889748136221982 1102 70-71 0.47198213664094624 1102 72-73 0.5197405399018962 1102 74-75 0.5850821230127146 1102 76-77 0.2745612713996586 1102 78-79 0.5762845196176123 1102 80-81 0.6171554351528208 1102 82-83 0.6377164918715721 1102 84-85 0.751907236277809 1102 86-87 1.0379465537134536 1102 88-89 0.7339876999553567 1102 90-91 0.6778033187393007 1102 92-93 0.7725106751026622 1102 94-95 0.8540608894005643 1102 96-97 0.797974247819834 1102 98-99 0.9707368807381762 1102 100-101 1.1921558281439637 1103 1 0.06405864339961198 1103 2 0.017473265168945318 1103 3 0.06440783148884321 1103 4 0.0384545586613001 1103 5 0.03295780837763829 1103 6 0.043914648763838215 1103 7 0.08214683867690553 1103 8 0.06792384291869524 1103 9 0.04844866808880255 1103 10-11 0.08706519542639768 1103 12-13 0.053967371265862596 1103 14-15 0.07422680315093544 1103 16-17 0.06320131398561557 1103 18-19 0.10695148170077573 1103 20-21 0.1414397176316129 1103 22-23 0.09349803396179368 1103 24-25 0.07187018577938176 1103 26-27 0.06361590588031163 1103 28-29 -0.0010026346147284926 1103 30-31 0.0807057824641575 1103 32-33 0.12561352105930723 1103 34-35 0.04505344638472053 1103 36-37 0.022656511594092876 1103 38-39 -0.04225193229618185 1103 40-41 0.1155634328112285 1103 42-43 0.19054758345254896 1103 44-45 0.13450354372866968 1103 46-47 0.1582051240071891 1103 48-49 0.12304367824177831 1103 50-51 0.02546554191476247 1103 52-53 0.11346552949519406 1103 54-55 0.26255283657641826 1103 56-57 0.2966225201779835 1103 58-59 0.20305442367070725 1103 60-61 0.0305149717232851 1103 62-63 0.21204223692490842 1103 64-65 0.2698151986705284 1103 66-67 0.20725992941198257 1103 68-69 0.23974727002197938 1103 70-71 0.2909922508517724 1103 72-73 0.3369933708321895 1103 74-75 0.3069050774452826 1103 76-77 0.16802001417602952 1103 78-79 0.44650699138793826 1103 80-81 0.3148513254921532 1103 82-83 0.3311359743934723 1103 84-85 0.35766595534476764 1103 86-87 0.5291653584888714 1103 88-89 0.5058858844277694 1103 90-91 0.4557409789242932 1103 92-93 0.4596758623604025 1103 94-95 0.69398734366019 1103 96-97 0.6022675136569831 1103 98-99 0.8464890308869322 1103 100-101 0.9749771679929253 1104 1 0.08063314138780697 1104 2 0.12445483691714543 1104 3 0.05763355113311519 1104 4 0.06329133431654554 1104 5 0.08013363942340845 1104 6 0.08937304006981606 1104 7 0.12305986090079557 1104 8 0.13035069161948343 1104 9 0.14223186526475473 1104 10-11 0.17583899750777476 1104 12-13 0.09798095096729043 1104 14-15 0.1771724208886809 1104 16-17 0.19700987615423315 1104 18-19 0.1336509714631191 1104 20-21 0.1918121723189401 1104 22-23 0.14089258445440578 1104 24-25 0.13520775346636071 1104 26-27 0.07595923283665229 1104 28-29 0.13833384490995826 1104 30-31 0.13040665534423823 1104 32-33 0.19944624372128317 1104 34-35 0.2890212551125799 1104 36-37 0.25822660992823643 1104 38-39 0.2534886350053682 1104 40-41 0.2517323729458525 1104 42-43 0.19353180392410252 1104 44-45 0.31242189865289305 1104 46-47 0.36751199635141774 1104 48-49 0.22912819178856836 1104 50-51 0.23176351624071145 1104 52-53 0.2106797870065762 1104 54-55 0.33754231924103095 1104 56-57 0.20164172753518272 1104 58-59 0.3094550448398081 1104 60-61 0.32459803046995006 1104 62-63 0.32103591007197707 1104 64-65 0.32948080616121445 1104 66-67 0.25465389234395275 1104 68-69 0.24963165571558932 1104 70-71 0.20029374701617542 1104 72-73 0.15793558894409898 1104 74-75 0.14346289458701378 1104 76-77 0.18750925359053383 1104 78-79 0.35794559247657176 1104 80-81 0.3542957399057194 1104 82-83 0.32288794507184804 1104 84-85 0.46984978896639973 1104 86-87 0.6087364921048604 1104 88-89 0.5470007337302185 1104 90-91 0.5894236562265895 1104 92-93 0.6730226547493778 1104 94-95 1.025335407530381 1104 96-97 0.870833989092251 1104 98-99 1.1222930782396219 1104 100-101 1.1330665265647326 1105 1 -0.002473561266675972 1105 2 0.020652391354026634 1105 3 0.03166366665217879 1105 4 0.015531052300744363 1105 5 0.01787430413592972 1105 6 -0.010101707772733448 1105 7 -0.026495905038828482 1105 8 -0.00532532019641252 1105 9 0.05156129258514852 1105 10-11 0.04808685712798422 1105 12-13 0.08180293418106999 1105 14-15 0.009153461288498477 1105 16-17 -0.03378146697106388 1105 18-19 0.051567378310508616 1105 20-21 0.08570093856807404 1105 22-23 0.04458417063398912 1105 24-25 -0.09124607759724768 1105 26-27 -0.11952096438324133 1105 28-29 -0.0057782331587148406 1105 30-31 -0.02589426544009399 1105 32-33 0.03163281633717219 1105 34-35 0.1392205573555998 1105 36-37 0.17958955734840032 1105 38-39 0.18005702361003983 1105 40-41 0.19798335153522828 1105 42-43 0.22705816211512087 1105 44-45 0.21264475531459226 1105 46-47 0.16511482219892315 1105 48-49 0.08150272183809193 1105 50-51 0.11638285066339193 1105 52-53 0.10015266870194495 1105 54-55 0.16416387597190152 1105 56-57 0.21709959926260325 1105 58-59 0.16337824946968027 1105 60-61 0.18591214514241727 1105 62-63 0.10912238096008053 1105 64-65 -0.07918494412098198 1105 66-67 -0.003254333041113 1105 68-69 0.05038673413928052 1105 70-71 0.21096236251703715 1105 72-73 0.08742836672693244 1105 74-75 0.04340739377682823 1105 76-77 -0.07074062465473929 1105 78-79 0.08083795561060825 1105 80-81 0.16688478015988295 1105 82-83 0.1861086021832321 1105 84-85 0.1070249769465903 1105 86-87 0.1734737351682547 1105 88-89 0.34936408396508156 1105 90-91 0.31919501906022063 1105 92-93 0.4229282094957618 1105 94-95 0.41848624911724386 1105 96-97 0.5003202808757372 1105 98-99 0.5609350977294802 1105 100-101 0.7187093118576726 1106 1 0.020754668853619762 1106 2 0.06836181410533726 1106 3 0.03681982474005707 1106 4 -0.019761137786375116 1106 5 0.01881595196046959 1106 6 0.05890862792987406 1106 7 0.13214774791091344 1106 8 0.07967143046514735 1106 9 0.07260275370341418 1106 10-11 0.1312277568067941 1106 12-13 0.10377627584914961 1106 14-15 0.009697470977634737 1106 16-17 -0.08894614047170535 1106 18-19 -0.04205222513263607 1106 20-21 0.048588527415887484 1106 22-23 0.004956755021872539 1106 24-25 0.11507769627961295 1106 26-27 0.09766615128825151 1106 28-29 0.07234221852408496 1106 30-31 0.1367437213897702 1106 32-33 0.2069496887402451 1106 34-35 -0.02207167785358166 1106 36-37 -9.699341590163613E-4 1106 38-39 0.14769742336945768 1106 40-41 0.2926741911521731 1106 42-43 0.21284945994106153 1106 44-45 0.26486617750682484 1106 46-47 0.2973282985068195 1106 48-49 0.33677106601814444 1106 50-51 0.2288638182343803 1106 52-53 0.3161580129144639 1106 54-55 0.34802800795282707 1106 56-57 0.33927802618659797 1106 58-59 0.28382594282457774 1106 60-61 0.33442665270943905 1106 62-63 0.3594578386985958 1106 64-65 0.45433593005836315 1106 66-67 0.5230108316846653 1106 68-69 0.5433111689992671 1106 70-71 0.5490019631758116 1106 72-73 0.6414273641368169 1106 74-75 0.6715329904747769 1106 76-77 0.40301154409295137 1106 78-79 0.5460570342071769 1106 80-81 0.5185500042046556 1106 82-83 0.5288369761988676 1106 84-85 0.4989770452151632 1106 86-87 0.7753847918883316 1106 88-89 0.8816267680782097 1106 90-91 1.0434708375440884 1106 92-93 1.0375831523145571 1106 94-95 1.1060798292388867 1106 96-97 0.938173931883032 1106 98-99 0.9784907010361579 1106 100-101 1.1914826208397642 1107 1 -0.07328926137010683 1107 2 -0.026808774988495543 1107 3 0.025023326147682212 1107 4 0.0071169805372193196 1107 5 -0.017525300403917754 1107 6 0.021376412423578017 1107 7 0.028400009566865947 1107 8 0.059250555003842464 1107 9 -0.029896254383181997 1107 10-11 0.04558907530585543 1107 12-13 0.03999737794965341 1107 14-15 0.02258087185713009 1107 16-17 0.06990415518119164 1107 18-19 0.04831688939221124 1107 20-21 0.08762302145466094 1107 22-23 0.06834442413076403 1107 24-25 0.04751345137586327 1107 26-27 0.02108103097656766 1107 28-29 0.063947842826515 1107 30-31 0.007511479708163904 1107 32-33 0.10862312069658486 1107 34-35 0.050998987204671664 1107 36-37 0.03928487351917909 1107 38-39 0.04590369439675612 1107 40-41 0.058100946051773406 1107 42-43 0.004816001974845108 1107 44-45 -0.07482454029160834 1107 46-47 0.0036318114678621782 1107 48-49 -0.01853338269012994 1107 50-51 -0.13408396696373615 1107 52-53 -0.14644044799881328 1107 54-55 -0.10611710944516517 1107 56-57 0.019791123579288694 1107 58-59 -0.05365281475285144 1107 60-61 0.0377034623210335 1107 62-63 0.1595828184534298 1107 64-65 0.09420505885822195 1107 66-67 0.0717486001983545 1107 68-69 0.08140357877383053 1107 70-71 0.20467319971494646 1107 72-73 0.1797954017652259 1107 74-75 0.10383766891739654 1107 76-77 0.021618705560975116 1107 78-79 0.1959187399993958 1107 80-81 0.16539247768314524 1107 82-83 0.004122923420805336 1107 84-85 0.2254828391367809 1107 86-87 0.22841939318983862 1107 88-89 0.43221228382114774 1107 90-91 0.36492942652692406 1107 92-93 0.495521087752536 1107 94-95 0.6018356138884151 1107 96-97 0.846761207054886 1107 98-99 0.8861642418561608 1107 100-101 1.0764177975978022 1108 1 0.05140940547595818 1108 2 0.10351976068498914 1108 3 0.11682046150497172 1108 4 0.08168502296492619 1108 5 -0.002545538779465062 1108 6 0.07121925200034696 1108 7 0.10534071966038994 1108 8 0.1382412649104907 1108 9 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9 1945.0 10 3200.0 11 5017.0 12 7434.0 13 9954.0 14 12308.0 15 15819.0 16 20580.0 17 28348.0 18 28687.0 19 22570.0 20 22028.0 21 23368.0 22 26208.0 23 29340.0 24 34265.0 25 40821.0 26 48769.0 27 58806.0 28 71193.0 29 86432.0 30 104690.0 31 127016.0 32 157863.0 33 200663.0 34 270156.0 35 388437.0 36 613874.0 37 894716.0 38 559892.0 39 27612.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.89677184487646 19.682466946446052 14.845593092835077 23.575168115842413 2 15.462278716995352 20.91953416746774 39.64531326190541 23.972873853631498 3 19.301844560710144 24.150983010911975 30.227005496685937 26.32016693169194 4 13.212712388957538 17.082174402437943 37.13857394509501 32.56653926350951 5 13.683798938495794 37.26637436546711 34.784916203242226 14.264910492794872 6 31.9244942944973 36.59851574011784 17.140952452918604 14.336037512466246 7 28.062132304370902 32.57658072511017 21.21243548804673 18.148851482472196 8 25.403629988368643 35.89467521069953 19.786801513014975 18.91489328791685 9 25.576693057622524 17.10471697658691 20.745735738652197 36.57285422713837 10-11 24.84816726843591 26.469926710008927 27.602304363293996 21.07960165826117 12-13 25.632491634017125 23.77657088650638 27.988545633751887 22.602391845724608 14-15 22.49109888936758 25.41557745709577 26.240393000421697 25.852930653114953 16-17 22.025986448016262 28.382559849861376 27.01979216471025 22.571661537412112 18-19 21.966828805675988 27.55954060120967 28.61010755946453 21.863523033649816 20-21 22.871622195585864 26.61903987905617 28.505566798667935 22.003771126690026 22-23 22.43254914420629 26.488716414670776 28.515925225596906 22.562809215526027 24-25 22.549942413636938 26.535192596082503 28.604726291078148 22.31013869920241 26-27 22.396268632494586 26.55773284025349 28.756712059494156 22.289286467757773 28-29 22.142601940487097 27.11131239113826 28.560122870975587 22.185962797399057 30-31 22.512208870515607 26.600351603299384 28.342849477729885 22.544590048455124 32-33 22.369663223070816 26.70649695244176 28.408245764265533 22.51559406022189 34-35 22.682062753373465 26.781040060063404 28.32900918589159 22.20788800067154 36-37 22.68992006622765 26.43169867636004 28.616574565457125 22.261806691955186 38-39 22.33046443839974 26.904715290623333 28.4494577266159 22.315362544361022 40-41 22.692307692307693 26.839190347464893 28.19629915149794 22.272202808729478 42-43 22.391813145034284 26.86506095497501 27.987937446041766 22.75518845394894 44-45 22.598972203443466 26.78846287461501 28.036883726520713 22.575681195420813 46-47 22.857165316633903 26.99184350980118 27.76017545354406 22.390815720020854 48-49 22.665242391008043 26.95678358828713 27.84444120425254 22.533532816452286 50-51 22.357491186090357 27.228948902209122 27.90569943969057 22.50786047200995 52-53 22.76121683243108 27.245781762017867 27.32726720861821 22.665734196932842 54-55 22.6676106316764 27.09682073632821 27.392815638511443 22.842752993483952 56-57 22.6107090159393 27.177621537819412 27.46651641664864 22.745153029592643 58-59 22.88295925213852 26.96588513390496 27.47605117939922 22.6751044345573 60-61 22.581574804068072 27.124631902563884 27.769577407787676 22.524215885580368 62-63 22.406555039993368 27.044953819380126 27.85143948099087 22.69705165963564 64-65 22.24336608624795 27.351669539028688 27.55734359193213 22.847620782791235 66-67 22.439221440042274 27.14445179543136 27.301075151570785 23.11525161295558 68-69 22.355453994381453 27.017007918382074 27.635081172513416 22.992456914723057 70-71 22.612654577690503 26.83454507095968 27.415923023555855 23.13687732779396 72-73 22.51582227525827 26.783760420869097 27.580978936005714 23.11943836786692 74-75 22.15217429340187 27.49035220933709 27.224367584437665 23.133105912823375 76-77 22.321184851065578 26.860796700134603 27.391578180878646 23.426440267921173 78-79 22.44811663926721 27.010423965052443 27.36714940932048 23.174309986359866 80-81 22.47459485077173 26.857500539793257 27.29240337510566 23.375501234329356 82-83 22.416320519397136 26.717134308859634 27.642305387725933 23.22423978401729 84-85 22.2669467967883 26.93095864268664 27.37273285478264 23.42936170574242 86-87 22.108430409448747 26.80427691169463 27.519519389948076 23.567773288908544 88-89 22.16731824722707 26.75225081818992 27.24152202427191 23.8389089103111 90-91 22.114890409455903 26.599805051304497 27.336603183177694 23.948701356061903 92-93 22.26024679174034 26.71896002915067 27.24161049829739 23.7791826808116 94-95 22.09502340179645 26.595598065984 27.384862717702003 23.924515814517544 96-97 21.781431811358416 26.40823927490486 27.664626761820525 24.145702151916197 98-99 21.853440810782097 26.581549220024396 27.29951372447193 24.265496244721575 100-101 22.004919579793185 25.655325886977977 28.02999115408788 24.309763379140968 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 545.0 1 389.0 2 276.0 3 342.5 4 334.5 5 338.0 6 542.5 7 1123.5 8 1879.5 9 2339.0 10 2255.5 11 2150.5 12 2344.5 13 2615.0 14 2992.0 15 3225.0 16 3472.5 17 3786.0 18 4094.5 19 4622.0 20 5374.5 21 6487.0 22 7996.5 23 10194.5 24 12828.0 25 16106.0 26 21315.5 27 27986.0 28 33597.5 29 38857.0 30 44179.0 31 51024.0 32 58131.0 33 64309.5 34 71943.5 35 80120.5 36 89761.0 37 98492.5 38 104399.0 39 109329.0 40 115557.5 41 120569.0 42 123031.5 43 126910.5 44 133150.5 45 138915.0 46 142168.0 47 153245.0 48 171262.0 49 170093.0 50 155538.5 51 148788.0 52 146126.5 53 143038.5 54 137290.5 55 126478.0 56 115068.5 57 104444.0 58 95279.0 59 87030.5 60 73988.5 61 59438.0 62 46387.0 63 34355.5 64 24782.5 65 17718.0 66 12291.5 67 8908.0 68 6628.5 69 4938.5 70 3452.0 71 2004.5 72 1152.5 73 730.5 74 454.0 75 260.5 76 155.5 77 79.0 78 43.5 79 25.0 80 12.0 81 8.0 82 5.5 83 4.0 84 5.0 85 3.0 86 3.0 87 3.5 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3944062973149994 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010142890505721986 16-17 0.002370900655712514 18-19 4.183942333610319E-4 20-21 0.0 22-23 0.0 24-25 1.7750058385013472E-4 26-27 2.5357226264304965E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012234861672527145 36-37 0.02244114524390989 38-39 0.011030393424972659 40-41 0.005806804814525836 42-43 0.00521090999731467 44-45 0.0016989341597084325 46-47 0.0010396462768365033 48-49 0.0031442960567738152 50-51 6.212520434754716E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.860740141934538E-4 60-61 0.0015214335758582977 62-63 0.0014580405101975353 64-65 7.733954010613013E-4 66-67 0.0028400093416021555 68-69 0.0026244729183555636 70-71 2.5357226264304965E-5 72-73 0.0 74-75 0.0 76-77 0.09152690820100877 78-79 0.0035500116770026944 80-81 2.4089364951089716E-4 82-83 0.0 84-85 1.3946474445367728E-4 86-87 0.0927186978354311 88-89 0.43865465714621155 90-91 0.3914141446158114 92-93 0.0 94-95 0.001052324889968656 96-97 2.5357226264304965E-5 98-99 0.0 100-101 4.944659121539467E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3943649.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.36569573437099 #Duplication Level Percentage of deduplicated Percentage of total 1 67.991587488701 30.164940830205744 2 15.342398086727844 13.613523307027265 3 6.201942214942315 8.25460443810845 4 3.196347867541876 5.672327878102737 5 1.8570902019518176 4.119554942553796 6 1.1869234670239526 3.159521123878164 7 0.79498708475657 2.468910858054521 8 0.6150424244480106 2.182942805343224 9 0.4445642731839343 1.7751062950605194 >10 2.1734694122873197 16.799977630946795 >50 0.12886209789205938 3.9106074277726797 >100 0.06238643892371817 4.953288261792424 >500 0.0028564556314953533 0.8529851335605566 >1k 0.0013139695454711807 1.221511987124506 >5k 1.713873320179795E-4 0.5610276763705211 >10k+ 5.7129110672659826E-5 0.2891694040980924 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11409 0.28930059444945533 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9029 0.22895039594040953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7787 0.19745672092014274 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5319 0.1348750864998381 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4697 0.1191028917634404 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4248 0.10771749717076748 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.5357226264304962E-5 0.0 0.0 0.0 0.0 6 2.5357226264304962E-5 0.0 0.0 0.0 7.607167879291489E-5 7 2.5357226264304962E-5 0.0 0.0 0.0 7.607167879291489E-5 8 2.5357226264304962E-5 0.0 0.0 0.0 7.607167879291489E-5 9 2.5357226264304962E-5 0.0 0.0 0.0 7.607167879291489E-5 10-11 2.5357226264304962E-5 0.0 0.0 1.2678613132152481E-5 1.0142890505721985E-4 12-13 2.5357226264304962E-5 0.0 0.0 2.5357226264304962E-5 1.7750058385013475E-4 14-15 2.5357226264304962E-5 0.0 0.0 2.5357226264304962E-5 2.1553642324659218E-4 16-17 3.803583939645744E-5 0.0 0.0 5.0714452528609924E-5 2.7892948890735457E-4 18-19 5.0714452528609924E-5 0.0 0.0 6.339306566076241E-5 3.0428671517165954E-4 20-21 5.0714452528609924E-5 0.0 0.0 7.607167879291489E-5 3.42322554568117E-4 22-23 7.607167879291489E-5 0.0 0.0 1.9017919698228724E-4 3.6767978083242193E-4 24-25 1.5214335758582977E-4 0.0 0.0 3.1696532830381203E-4 3.803583939645744E-4 26-27 1.5214335758582977E-4 0.0 0.0 7.100023354005389E-4 3.930370070967269E-4 28-29 2.028578101144397E-4 0.0 0.0 0.003055545764848748 4.057156202288794E-4 30-31 2.2821503637874467E-4 0.0 0.0 0.009787889338021716 4.183942333610319E-4 32-33 2.2821503637874467E-4 0.0 0.0 0.02264400305402433 4.3107284649318437E-4 34-35 2.4089364951089716E-4 0.0 0.0 0.043272106620036416 5.071445252860993E-4 36-37 2.5357226264304965E-4 0.0 0.0 0.0766041805444653 5.071445252860993E-4 38-39 2.5357226264304965E-4 0.0 0.0 0.12525201913253436 5.071445252860993E-4 40-41 2.5357226264304965E-4 0.0 0.0 0.18192541983325594 5.071445252860993E-4 42-43 2.6625087577520213E-4 0.0 0.0 0.24302365651709879 5.071445252860993E-4 44-45 3.0428671517165954E-4 0.0 0.0 0.3167624704936976 5.071445252860993E-4 46-47 3.6767978083242193E-4 0.0 0.0 0.4071483035128126 5.451803646825566E-4 48-49 4.3107284649318437E-4 0.0 0.0 0.5014898638291592 6.59287882871929E-4 50-51 4.3107284649318437E-4 0.0 0.0 0.6082437864018831 6.84645109136234E-4 52-53 4.3107284649318437E-4 0.0 0.0 0.7147948511644926 7.480381747969963E-4 54-55 4.3107284649318437E-4 0.0 0.0 0.8370673962109711 7.860740141934538E-4 56-57 4.8178729902179426E-4 0.0 0.0 0.9874230693451673 7.987526273256063E-4 58-59 4.8178729902179426E-4 0.0 0.0 1.1552879072148663 8.241098535899113E-4 60-61 4.8178729902179426E-4 0.0 0.0 1.3353368923045636 8.367884667220638E-4 62-63 4.8178729902179426E-4 0.0 0.0 1.5208376810410866 8.621456929863687E-4 64-65 5.071445252860993E-4 0.0 0.0 1.7180915441511149 9.001815323828262E-4 66-67 5.578589778147091E-4 0.0 0.0 1.948335158631004 9.508959849114361E-4 68-69 5.578589778147091E-4 0.0 0.0 2.2122278123636256 9.889318243078936E-4 70-71 5.832162040790141E-4 0.0 0.0 2.496849998567317 0.0010396462768365033 72-73 6.212520434754716E-4 0.0 0.0 2.7871648820673443 0.0011283965687615707 74-75 6.59287882871929E-4 2.5357226264304962E-5 0.0 3.098247333877837 0.0012298254738187906 76-77 6.59287882871929E-4 2.5357226264304962E-5 0.0 3.4397711358186287 0.001293218539479553 78-79 6.59287882871929E-4 2.5357226264304962E-5 0.0 3.830614742843493 0.001318575765743858 80-81 7.10002335400539E-4 2.5357226264304962E-5 0.0 4.252913989049228 0.001318575765743858 82-83 7.353595616648439E-4 2.5357226264304962E-5 0.0 4.684595408972756 0.001343932992008163 84-85 7.480381747969963E-4 2.5357226264304962E-5 0.0 5.138578509395741 0.001343932992008163 86-87 7.733954010613013E-4 2.5357226264304962E-5 0.0 5.614964719223237 0.001394647444536773 88-89 7.860740141934538E-4 2.5357226264304962E-5 0.0 6.135117501582925 0.0014707191233296877 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 650 0.0 26.28293 3 TACATGG 10490 0.0 20.402609 2 GTACATG 11135 0.0 20.279158 1 GAGTACT 10770 0.0 19.983347 12-13 AGAGTAC 16015 0.0 19.557962 10-11 ACATGGG 10755 0.0 18.929167 3 GTACTTT 11275 0.0 18.91994 14-15 GTATCAA 24685 0.0 18.468882 1 AGTACTT 11490 0.0 18.462654 12-13 CGCCGTT 130 0.007607505 18.252035 7 GGTATCA 19990 0.0 17.206247 1 CATGGGG 7375 0.0 16.923038 4 CTTTGCG 1070 0.0 16.85328 2 CAACGCA 27235 0.0 16.535734 5 ATCAACG 27390 0.0 16.39018 3 TCAACGC 27555 0.0 16.343702 4 TATCAAC 27690 0.0 16.212605 2 AACGCAG 27825 0.0 16.202166 6 GAGTACA 9465 0.0 16.106127 1 GGACCGA 860 0.0 16.002365 6 >>END_MODULE