FastQCFastQC Report
Fri 27 May 2016
SRR938994_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938994_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3959899
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104620.26419865759202443No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91760.23172308182607687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72030.1818985787263766No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58680.1481855976629707No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45220.11419483173687006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43820.1106593880298462No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41420.10459862738923392No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG109950.020.9561861
TACATGG106000.020.8939272
AGAGTAC146850.020.51105910-11
GAGTACT101650.019.83247212-13
GTATCAA238700.019.7823621
ACATGGG110250.019.1870843
AGTACTT105450.019.07290812-13
GTACTTT107300.018.7007914-15
TATACCG2102.0213498E-518.0281335
CAACGCA261250.017.8789045
ATCAACG265350.017.584823
CATGGGG75300.017.5343364
AACGCAG267100.017.5039356
TATCAAC267950.017.4671732
TCAACGC267950.017.4671734
GTATACG2504.648982E-617.067651
AGTACAT80400.016.4220832
GAGTACA89100.016.335481
GGTATCA192100.016.1900311
ACGCAGA288400.015.9158247
Produced by FastQC (version 0.11.5)