##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938994_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3959899 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76006281978404 33.0 31.0 34.0 30.0 34.0 2 31.836186225961825 33.0 31.0 34.0 30.0 34.0 3 31.895109698504935 34.0 31.0 34.0 30.0 34.0 4 35.41971701803506 37.0 35.0 37.0 33.0 37.0 5 35.455177518416505 37.0 35.0 37.0 33.0 37.0 6 35.52712379785444 37.0 35.0 37.0 33.0 37.0 7 35.4359484926257 37.0 35.0 37.0 33.0 37.0 8 35.41605303569611 37.0 35.0 37.0 33.0 37.0 9 37.026917100663425 39.0 37.0 39.0 33.0 39.0 10-11 36.91303515569463 39.0 37.0 39.0 33.0 39.0 12-13 36.79246276230783 39.0 37.0 39.0 32.0 39.0 14-15 38.01743743969227 40.0 38.0 41.0 33.0 41.0 16-17 37.944204132479136 40.0 38.0 41.0 33.0 41.0 18-19 37.870412477691985 40.0 38.0 41.0 32.5 41.0 20-21 37.735048040366685 40.0 38.0 41.0 32.0 41.0 22-23 37.81232475878804 40.0 38.0 41.0 32.0 41.0 24-25 37.75228092938734 40.0 38.0 41.0 32.0 41.0 26-27 37.6745492751204 40.0 38.0 41.0 32.0 41.0 28-29 37.5497274046636 40.0 37.0 41.0 32.0 41.0 30-31 37.383005601910554 40.0 37.0 41.0 31.0 41.0 32-33 37.211260943776594 40.0 37.0 41.0 31.0 41.0 34-35 36.89587007143364 40.0 36.0 41.0 30.0 41.0 36-37 36.64509599866057 40.0 36.0 41.0 30.0 41.0 38-39 36.4479452632504 39.0 36.0 41.0 30.0 41.0 40-41 36.30875257171964 39.0 35.5 41.0 30.0 41.0 42-43 36.10929887353188 39.0 35.0 41.0 29.0 41.0 44-45 36.07826209708884 39.0 35.0 41.0 29.0 41.0 46-47 35.89842430324612 39.0 35.0 41.0 28.0 41.0 48-49 35.7417074273864 39.0 35.0 41.0 27.0 41.0 50-51 34.582434046928974 38.0 34.0 40.0 25.0 40.5 52-53 34.730255367624274 38.0 34.5 39.5 26.0 40.5 54-55 35.231508429886716 39.0 35.0 40.5 26.0 41.0 56-57 35.12105106721157 39.0 35.0 41.0 26.0 41.0 58-59 34.91521867098126 38.0 34.5 40.0 26.0 41.0 60-61 34.641454239110644 38.0 34.0 40.0 25.5 41.0 62-63 34.286992041968745 37.5 34.0 40.0 24.5 41.0 64-65 33.882918352210496 37.0 33.5 40.0 23.0 41.0 66-67 33.49494482056234 36.5 33.0 39.5 22.5 41.0 68-69 33.09949180522028 36.0 33.0 39.0 22.0 41.0 70-71 32.685660543362346 35.5 32.0 39.0 21.0 40.0 72-73 32.248052286182045 35.0 32.0 38.0 20.0 40.0 74-75 31.786836608711482 35.0 31.5 37.0 18.5 39.5 76-77 31.3775928375951 35.0 31.0 37.0 17.5 39.0 78-79 30.94579381948883 35.0 31.0 36.0 14.0 39.0 80-81 30.510175385786354 34.5 30.5 36.0 9.5 37.5 82-83 30.129991194219855 34.0 30.0 35.0 7.0 37.0 84-85 29.77620843359894 34.0 30.0 35.0 3.5 37.0 86-87 29.392521374913855 34.0 29.5 35.0 2.0 36.0 88-89 28.981152928395396 34.0 29.0 35.0 2.0 36.0 90-91 28.557277723497492 34.0 29.0 35.0 2.0 35.5 92-93 28.135298400287482 34.0 29.0 35.0 2.0 35.0 94-95 27.657583438365474 34.0 27.0 35.0 2.0 35.0 96-97 27.080170100298012 33.5 26.5 35.0 2.0 35.0 98-99 26.35818400923862 33.0 25.0 35.0 2.0 35.0 100-101 23.7330986219598 30.5 11.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19289407556977523 1101 2 0.23892990278762483 1101 3 0.22710931064820983 1101 4 0.07216108555893896 1101 5 0.010042842396501328 1101 6 0.10744304136397886 1101 7 0.11743500649961192 1101 8 0.2340759477019816 1101 9 0.1076267133783233 1101 10-11 0.2373761222201125 1101 12-13 0.3744933977075249 1101 14-15 0.14009675455083936 1101 16-17 0.3505479587079776 1101 18-19 0.352754419670255 1101 20-21 0.43352673130682007 1101 22-23 0.4289505131490259 1101 24-25 0.47368847282251636 1101 26-27 0.38625223498538475 1101 28-29 0.38457583616366264 1101 30-31 0.4271662759574184 1101 32-33 0.34390596453519606 1101 34-35 0.1814263982698776 1101 36-37 0.14555720137978767 1101 38-39 0.17078935468629908 1101 40-41 0.10930877202625311 1101 42-43 0.13227841844646093 1101 44-45 0.15995375343405271 1101 46-47 0.1286405135463582 1101 48-49 0.20334134309461405 1101 50-51 0.18239080040365252 1101 52-53 0.18036981270165597 1101 54-55 0.20073931101189402 1101 56-57 0.1916775251767575 1101 58-59 0.12476103262515181 1101 60-61 0.08140683709658703 1101 62-63 0.09386049217136616 1101 64-65 0.011136815976819037 1101 66-67 0.026555685180049693 1101 68-69 0.05313355457632696 1101 70-71 0.041912136417501245 1101 72-73 -0.056017743092844796 1101 74-75 -0.08864778774285753 1101 76-77 -0.09832972204290868 1101 78-79 -0.20013365731862365 1101 80-81 -0.2325650130017678 1101 82-83 -0.2748971312509596 1101 84-85 -0.38286356113344766 1101 86-87 -0.3979331101036543 1101 88-89 -0.37127200800802385 1101 90-91 -0.4621165012958173 1101 92-93 -0.4416645856218224 1101 94-95 -0.51055137098475 1101 96-97 -0.5006154480545035 1101 98-99 -0.7684666805094906 1101 100-101 -0.4470864261329588 1102 1 0.3100068892138488 1102 2 0.22670231103836613 1102 3 0.28880860522808405 1102 4 0.3036742563777608 1102 5 0.3910205011160315 1102 6 0.36523863025584546 1102 7 0.36357912651033075 1102 8 0.34032836309621217 1102 9 0.48286043067450635 1102 10-11 0.31517874745451024 1102 12-13 0.4311358479262921 1102 14-15 0.4801991922685005 1102 16-17 0.4268626296607465 1102 18-19 0.5068241758984939 1102 20-21 0.5497594663724499 1102 22-23 0.5052766136937166 1102 24-25 0.6143091710991513 1102 26-27 0.6120137136888317 1102 28-29 0.6015984274664561 1102 30-31 0.547411726444075 1102 32-33 0.5485099470336365 1102 34-35 0.5193798584002423 1102 36-37 0.4782985890036855 1102 38-39 0.3948255007707715 1102 40-41 0.47757872470059937 1102 42-43 0.3215105284562796 1102 44-45 0.488600311162422 1102 46-47 0.4998731593183052 1102 48-49 0.46065585331873393 1102 50-51 0.4674577528101125 1102 52-53 0.4027846354536919 1102 54-55 0.44427094776518317 1102 56-57 0.38504707602951527 1102 58-59 0.4203529961933228 1102 60-61 0.24525121605899614 1102 62-63 0.2204574740447427 1102 64-65 0.2815251720800447 1102 66-67 0.2838247627762627 1102 68-69 0.3038136510135061 1102 70-71 0.27935165780333904 1102 72-73 0.2643693271812779 1102 74-75 0.3050343624491205 1102 76-77 0.28808270763831345 1102 78-79 0.2635148470121216 1102 80-81 0.033437701526313646 1102 82-83 -0.08825921715148866 1102 84-85 -0.08098607207192643 1102 86-87 -0.03307046165290117 1102 88-89 -0.11067884790444182 1102 90-91 -0.1375747029209613 1102 92-93 0.0025636490419884694 1102 94-95 -0.10768693666025442 1102 96-97 -0.24542434170572136 1102 98-99 -0.2505405442473254 1102 100-101 -0.01513168017349642 1103 1 0.014998956458402546 1103 2 0.11624326191832068 1103 3 0.20414162335259078 1103 4 0.20627788127925584 1103 5 0.23797127600439438 1103 6 0.1746764027801433 1103 7 0.1683009547215164 1103 8 0.08164845765886497 1103 9 0.2243830994425693 1103 10-11 0.22014360167042923 1103 12-13 0.3158827877739441 1103 14-15 0.26729937614031485 1103 16-17 0.3771216262459802 1103 18-19 0.41366168574877094 1103 20-21 0.3430909084528295 1103 22-23 0.39314947946729717 1103 24-25 0.3438385643221622 1103 26-27 0.3148715019735775 1103 28-29 0.30037751861434003 1103 30-31 0.2433039250256357 1103 32-33 0.27691026224245263 1103 34-35 0.3209153456605591 1103 36-37 0.3119880032406144 1103 38-39 0.3041046181860594 1103 40-41 0.3092641462924064 1103 42-43 0.06746608401184062 1103 44-45 0.2768192762267816 1103 46-47 0.21204043013521812 1103 48-49 0.24962259878761017 1103 50-51 0.12422518123143789 1103 52-53 0.19571481669876079 1103 54-55 0.32145392648858007 1103 56-57 0.3158690355777125 1103 58-59 0.23727466680010423 1103 60-61 0.23757913830748123 1103 62-63 0.24578902119473867 1103 64-65 0.1605097793823802 1103 66-67 0.044837318594488806 1103 68-69 0.06271693443878235 1103 70-71 0.07079804603554152 1103 72-73 0.008961814704271376 1103 74-75 0.044200319895935536 1103 76-77 -0.08801668821143593 1103 78-79 -0.08060987061330493 1103 80-81 -0.06679061473320758 1103 82-83 -0.05917962692296186 1103 84-85 -0.1947811863351241 1103 86-87 -0.03658041699831571 1103 88-89 -0.08826235197588872 1103 90-91 -0.12582655477281435 1103 92-93 -0.15483482744868837 1103 94-95 -0.18212570734583267 1103 96-97 -0.3197252610647965 1103 98-99 -0.3876974657077952 1103 100-101 -0.16173835209364285 1104 1 0.10986660485366073 1104 2 0.12174012041171878 1104 3 0.13046716236341638 1104 4 0.08644624163315484 1104 5 0.11171096978375061 1104 6 0.152972806137889 1104 7 0.17876185426177926 1104 8 0.20111783123734028 1104 9 0.2613952384680829 1104 10-11 0.09785826298900702 1104 12-13 0.22567044508269163 1104 14-15 0.279689871573396 1104 16-17 0.1386593647844947 1104 18-19 0.23358183782582387 1104 20-21 0.15960127785217537 1104 22-23 0.20073422296175636 1104 24-25 0.20542491622816073 1104 26-27 0.25151197593212515 1104 28-29 0.2618217028745704 1104 30-31 0.35732810348461186 1104 32-33 0.3676989939004045 1104 34-35 0.3387949189584347 1104 36-37 0.2772397159125859 1104 38-39 0.2757255534300711 1104 40-41 0.40629636556420934 1104 42-43 0.4200200597885555 1104 44-45 0.43446508209449064 1104 46-47 0.4537413004452162 1104 48-49 0.47341246206017473 1104 50-51 0.39736570436356544 1104 52-53 0.37787489348423264 1104 54-55 0.37370707204110687 1104 56-57 0.40420157839918147 1104 58-59 0.2615829066725226 1104 60-61 0.32652915181465403 1104 62-63 0.19516015966875955 1104 64-65 0.15546450857292626 1104 66-67 0.25806046577783803 1104 68-69 0.22475288218781486 1104 70-71 0.20921200535467932 1104 72-73 0.13250792644405607 1104 74-75 0.15226111337066328 1104 76-77 0.020061222646212684 1104 78-79 0.11465776433702857 1104 80-81 0.107221587782238 1104 82-83 0.035722878688428494 1104 84-85 0.060127145410959315 1104 86-87 0.05166529400797515 1104 88-89 0.009688292695873457 1104 90-91 0.08292366464091572 1104 92-93 0.11524821986873945 1104 94-95 0.06147114654142882 1104 96-97 0.14767981837853483 1104 98-99 0.05401722299859202 1104 100-101 0.3727718479992248 1105 1 0.10933160378162654 1105 2 0.1129051438995532 1105 3 0.14130320562296816 1105 4 0.09897720083651507 1105 5 0.12476513155640845 1105 6 0.19026285551123578 1105 7 0.2101781478855571 1105 8 0.2029293760586981 1105 9 0.1770691696220581 1105 10-11 0.13060568538909934 1105 12-13 0.2487700826245529 1105 14-15 0.36700774235863776 1105 16-17 0.3268928468467749 1105 18-19 0.3283378508920549 1105 20-21 0.29201112745924007 1105 22-23 0.2912603032808079 1105 24-25 0.3058473975563558 1105 26-27 0.2779041077140718 1105 28-29 0.3047286532369071 1105 30-31 0.30196844375885945 1105 32-33 0.08822494402907921 1105 34-35 0.22245473177993347 1105 36-37 0.2556605256506046 1105 38-39 0.22660326710264656 1105 40-41 0.19726151292276484 1105 42-43 0.18467213889493905 1105 44-45 0.19773819424251116 1105 46-47 0.30269276615150886 1105 48-49 0.2764490372830224 1105 50-51 0.3031685606047745 1105 52-53 0.27112495651129365 1105 54-55 0.27159940514349046 1105 56-57 0.3672751127708622 1105 58-59 0.3208719763632999 1105 60-61 0.35337610231532324 1105 62-63 0.269479742050585 1105 64-65 0.3573738114482268 1105 66-67 0.2610523016208006 1105 68-69 0.17728035201788828 1105 70-71 0.11219179858795769 1105 72-73 0.23519473708770988 1105 74-75 0.3692487671190534 1105 76-77 0.34511139679618097 1105 78-79 0.3167314664644323 1105 80-81 0.2588231938432486 1105 82-83 0.2440819280111839 1105 84-85 0.3404641811686986 1105 86-87 0.4536634382526721 1105 88-89 0.4707339085553386 1105 90-91 0.49763659418608697 1105 92-93 0.5624390031496347 1105 94-95 0.6319285839852924 1105 96-97 0.5021037551266261 1105 98-99 0.6866991501470423 1105 100-101 0.6935196031984674 1106 1 0.2192606062402298 1106 2 0.2313925474206897 1106 3 0.24880093136717107 1106 4 0.21462115600091636 1106 5 0.21252348158879641 1106 6 0.17851824656280257 1106 7 0.20321324778473837 1106 8 0.206311645042085 1106 9 0.13181061909519798 1106 10-11 0.2600367125110381 1106 12-13 0.31961553274986443 1106 14-15 0.3340024810660296 1106 16-17 0.4489719136621204 1106 18-19 0.33777032183888167 1106 20-21 0.42211218990790655 1106 22-23 0.3457850069790922 1106 24-25 0.4246903975465628 1106 26-27 0.34765544030754114 1106 28-29 0.38088379916909787 1106 30-31 0.3977824698493535 1106 32-33 0.4598545856084826 1106 34-35 0.25315347950336076 1106 36-37 0.24255182641375939 1106 38-39 0.3223075836758724 1106 40-41 0.3800671796509292 1106 42-43 0.42862001800749283 1106 44-45 0.4806192217482348 1106 46-47 0.5102221144399195 1106 48-49 0.4770668844937944 1106 50-51 0.503066248870411 1106 52-53 0.3025939537133624 1106 54-55 0.2631182242297214 1106 56-57 0.4001760242994621 1106 58-59 0.4421270969127491 1106 60-61 0.40312576213641904 1106 62-63 0.2933942332088435 1106 64-65 0.26317806690703804 1106 66-67 0.4504748484220329 1106 68-69 0.4202290672519453 1106 70-71 0.3811874919931313 1106 72-73 0.4102296218027206 1106 74-75 0.312763584785543 1106 76-77 0.31623728112238325 1106 78-79 0.37371950614195626 1106 80-81 0.4696842243080894 1106 82-83 0.3665975831225765 1106 84-85 0.440294846050719 1106 86-87 0.5609169312898885 1106 88-89 0.520952943805046 1106 90-91 0.5648399596347105 1106 92-93 0.5716294249926541 1106 94-95 0.7023275203455697 1106 96-97 0.7752021742243898 1106 98-99 0.8329473625700778 1106 100-101 1.2669350550097818 1107 1 0.11640518983412562 1107 2 0.03584164318213112 1107 3 0.10848381977476862 1107 4 0.1380190605242433 1107 5 0.06939990463374812 1107 6 0.10746339021846296 1107 7 0.03578509133316743 1107 8 0.16980709535894078 1107 9 0.1736179801689417 1107 10-11 0.11993704467602129 1107 12-13 0.1427491710633504 1107 14-15 0.21149299802939936 1107 16-17 0.2552008062262985 1107 18-19 0.19230100485592772 1107 20-21 0.24182655788405327 1107 22-23 0.21523871752582124 1107 24-25 0.2575985584727576 1107 26-27 0.3186577106299566 1107 28-29 0.3001356013979688 1107 30-31 0.3220304026272629 1107 32-33 0.3777086894926356 1107 34-35 0.22519302859312518 1107 36-37 0.10466320791778116 1107 38-39 0.29938622810529836 1107 40-41 0.2363100357720569 1107 42-43 0.28636035426985984 1107 44-45 0.39734219237578117 1107 46-47 0.36394602872097437 1107 48-49 0.22480733602754555 1107 50-51 0.34949286199525886 1107 52-53 0.19367837323940051 1107 54-55 0.2382674810305403 1107 56-57 0.24084165500818244 1107 58-59 0.27219882846454624 1107 60-61 0.23684190345809952 1107 62-63 0.12313505072926034 1107 64-65 0.27463119638117206 1107 66-67 0.14359123401425933 1107 68-69 0.17815145371552177 1107 70-71 0.23744315240780622 1107 72-73 0.17613933312910746 1107 74-75 0.13061016328182262 1107 76-77 0.21547358196918154 1107 78-79 0.24930568735539538 1107 80-81 0.109301717160605 1107 82-83 0.20780688871986897 1107 84-85 0.2034555967189675 1107 86-87 0.2846947860316966 1107 88-89 0.35125616781482094 1107 90-91 0.34821069940718985 1107 92-93 0.2686803964291897 1107 94-95 0.4389981852702398 1107 96-97 0.42374710871487764 1107 98-99 0.5740138953822083 1107 100-101 0.7668143475263918 1108 1 0.24954875170297086 1108 2 0.2152809011166923 1108 3 0.25087092028465463 1108 4 0.28825563148654965 1108 5 0.26890666299592425 1108 6 0.2838697877841341 1108 7 0.25690549726751044 1108 8 0.25498388964103924 1108 9 0.2942073410313384 1108 10-11 0.29638710254589284 1108 12-13 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-2.354688080155249 2316 94-95 -2.5951148413622747 2316 96-97 -2.8103312375346903 2316 98-99 -3.159376399763289 2316 100-101 -3.568378686391622 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12832.0 3 3400.0 4 3514.0 5 3870.0 6 4567.0 7 5519.0 8 6723.0 9 8637.0 10 10307.0 11 12695.0 12 16554.0 13 23408.0 14 22297.0 15 19973.0 16 23806.0 17 30018.0 18 27285.0 19 21802.0 20 21996.0 21 24133.0 22 26757.0 23 29811.0 24 34288.0 25 40012.0 26 45997.0 27 54102.0 28 63807.0 29 75617.0 30 91542.0 31 110994.0 32 138250.0 33 177125.0 34 235681.0 35 337729.0 36 525460.0 37 810297.0 38 738066.0 39 120997.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.9428218683719 18.977340213365412 14.302168476368356 23.777669441894332 2 15.075473920412813 20.336760128862473 40.467781095553455 24.119984855171257 3 19.33912999519753 23.905037535070647 30.010413770442078 26.74541869928974 4 12.85456649643329 16.475425874158756 37.54237506654876 33.12763256285919 5 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28.54464629771413 22.475845202973115 26-27 22.39569169297345 26.451519345520868 28.736780075040862 22.416008886464816 28-29 22.21915164154002 27.004988752488245 28.498972338731516 22.276887267240223 30-31 22.52401404259601 26.55384014841505 28.279091792119825 22.643054016869115 32-33 22.336503140418394 26.65682998814214 28.37336957559068 22.633297295848788 34-35 22.713958826891258 26.73528217708272 28.24850844159128 22.302250554434735 36-37 22.68212923574491 26.381239871724706 28.55457612290733 22.382054769623057 38-39 22.367894719598432 26.7944143439874 28.409019622484138 22.42867131393003 40-41 22.695771178287412 26.75233403138158 28.181079731733167 22.37081505859784 42-43 22.38401965152471 26.805625408935903 27.958524677984204 22.851830261555182 44-45 22.56852702395836 26.751841670912803 28.009527259167967 22.670104045960873 46-47 22.832435675092626 26.92548041848699 27.762421380033825 22.479662526386555 48-49 22.650262556036555 26.854131329587023 27.84836655368116 22.64723956069527 50-51 22.328744662429845 27.130662244161456 27.985829220362003 22.554763873046703 52-53 22.719801661415868 27.158961084816546 27.38618170918427 22.735055544583314 54-55 22.63230837828711 27.00358474557222 27.51726239075238 22.846844485388292 56-57 22.61175059361373 27.08901120749831 27.522360021807103 22.77687817708086 58-59 22.856548338693642 26.872583941316712 27.565930654156556 22.70493706583309 60-61 22.627602928181588 26.99890063764032 27.826670754169687 22.546825680008407 62-63 22.39654411168069 26.96371807422023 27.94361362500148 22.696124189097606 64-65 22.274413621624973 27.261211644974164 27.65588634384893 22.808488389551933 66-67 22.489845755595077 27.02025665518916 27.37391948180608 23.115978107409678 68-69 22.351440984212214 26.931179543314393 27.73632257962879 22.981056892844602 70-71 22.626015038300828 26.659820026701386 27.604074496084035 23.110090438913748 72-73 22.53933439513991 26.67148790198521 27.766136127532643 23.02304157534224 74-75 22.223536251825546 27.366005924311644 27.444712204440197 22.965745619422616 76-77 22.401315881385756 26.810905426677156 27.490703812637022 23.297074879300066 78-79 22.52821806291245 26.92861311325369 27.52358848736555 23.019580336468305 80-81 22.58433142228962 26.810580917420335 27.432231972341608 23.172855687948438 82-83 22.559455176232152 26.670446637645973 27.799790217959984 22.970307968161894 84-85 22.50495842819763 26.83211651763336 27.547499246567835 23.115425807601177 86-87 22.336628719627438 26.774650664967396 27.695594537830864 23.193126077574302 88-89 22.4362173911166 26.71429612870598 27.427683916756777 23.421802563420645 90-91 22.4160608873185 26.574373199540023 27.56678476765294 23.442781145488535 92-93 22.618524667841296 26.6488016371972 27.524120330437412 23.20855336452409 94-95 22.505801230354987 26.559593152318772 27.538996577378892 23.39560903994735 96-97 22.247143597168506 26.33426461393689 27.93106321724464 23.487528571649964 98-99 22.337720409782285 26.512245640711967 27.544336021981746 23.605697927523998 100-101 22.564536527538692 25.870086431409245 27.927401375556627 23.637975665495432 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3754.0 1 2124.5 2 527.0 3 560.5 4 518.5 5 525.5 6 825.0 7 1527.5 8 2452.5 9 2966.0 10 2921.5 11 2970.0 12 3383.5 13 3783.0 14 4136.0 15 4640.0 16 5077.5 17 5380.5 18 5609.0 19 5887.0 20 6395.0 21 7254.0 22 8477.5 23 10386.5 24 12867.5 25 16174.0 26 21261.5 27 27702.0 28 33132.0 29 38276.0 30 43740.0 31 50660.5 32 57994.0 33 64876.0 34 72092.5 35 80114.5 36 90291.5 37 98467.0 38 103959.0 39 109241.0 40 115093.5 41 120189.0 42 122970.5 43 126733.0 44 133231.5 45 138794.0 46 142323.0 47 153230.5 48 171051.5 49 170072.5 50 154977.5 51 147424.0 52 144739.5 53 142012.5 54 136449.5 55 125829.5 56 113736.5 57 103020.5 58 95064.5 59 86865.5 60 73762.5 61 60003.5 62 47032.0 63 34981.0 64 25513.0 65 18618.0 66 13236.0 67 9512.5 68 7057.0 69 5226.0 70 3710.5 71 2340.5 72 1396.0 73 886.5 74 570.5 75 343.5 76 235.5 77 136.0 78 87.0 79 79.5 80 63.0 81 46.0 82 41.0 83 31.0 84 20.5 85 16.5 86 14.0 87 14.0 88 13.0 89 10.5 90 9.5 91 8.0 92 7.0 93 5.0 94 3.5 95 3.5 96 1.5 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2847042310927627 2 0.08669413033009175 3 0.0908861564398486 4 0.10487641225192863 5 0.10664413410544056 6 0.08265362323635023 7 0.09199729589062751 8 0.08863862436895487 9 0.09346197971210883 10-11 0.086858275930775 12-13 0.0912775805645548 14-15 0.09948486059871729 16-17 0.08885327630830989 18-19 0.09901767696600342 20-21 0.10100005075887038 22-23 0.11386654053550356 24-25 0.1260385681553999 26-27 0.12930885358439698 28-29 0.12126571915091774 30-31 0.11376552785816003 32-33 0.1084749888822922 34-35 0.3817142810965633 36-37 0.586113433701213 38-39 0.5689033987988077 40-41 0.5318191196290613 42-43 0.5577793777063506 44-45 0.580444097185307 46-47 0.5688150127061322 48-49 0.5910756814757144 50-51 0.5824769773168457 52-53 0.5857851424998466 54-55 0.5757596342735004 56-57 0.5488018760074437 58-59 0.5438901345716141 60-61 0.49074483970424504 62-63 0.565355328507116 64-65 0.5996617590499151 66-67 0.6118716664238154 68-69 0.6142833440953923 70-71 0.6098261597076087 72-73 0.6020355569674883 74-75 0.5996491324652472 76-77 0.5825906165788571 78-79 0.5599385236845688 80-81 0.5555318456354569 82-83 0.5676659935013494 84-85 0.578689001916463 86-87 0.5688402658754681 88-89 0.5820855531921395 90-91 0.5954318531861545 92-93 0.6036517598049849 94-95 0.6030583103255917 96-97 0.5995607463725716 98-99 0.6037148927283247 100-101 0.581327958112063 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3959899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.30572917375974 #Duplication Level Percentage of deduplicated Percentage of total 1 69.86413747411659 32.351098288347615 2 14.600528134628515 13.521762031919348 3 5.7611108009931895 8.00317309472438 4 3.0346995086050015 5.6209589427681985 5 1.7497427873429423 4.051155781722088 6 1.0961737829070723 3.0455475791202353 7 0.7611839698432291 2.4673025131277506 8 0.5431264953574677 2.0119894720892937 9 0.4268544664395717 1.778922658760447 >10 1.9853401697084734 15.993380984512196 >50 0.11568101998190031 3.675089703173092 >100 0.05727050992992753 4.656263316275206 >500 0.002730842251460055 0.8427376402386038 >1k 0.00120157052622014 1.1533836042197831 >5k 1.6385052630274617E-4 0.562642803268885 >10k+ 5.4616842100915394E-5 0.26459158573286623 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10462 0.26419865759202443 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9176 0.23172308182607687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7203 0.1818985787263766 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5868 0.1481855976629707 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4522 0.11419483173687006 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4382 0.1106593880298462 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4142 0.10459862738923392 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5253169335884575E-5 2 2.5253169335884575E-5 0.0 0.0 0.0 2.5253169335884575E-5 3 2.5253169335884575E-5 0.0 0.0 0.0 2.5253169335884575E-5 4 2.5253169335884575E-5 0.0 0.0 0.0 2.5253169335884575E-5 5 5.050633867176915E-5 0.0 0.0 0.0 2.5253169335884575E-5 6 5.050633867176915E-5 0.0 0.0 0.0 2.5253169335884575E-5 7 5.050633867176915E-5 0.0 0.0 0.0 2.5253169335884575E-5 8 5.050633867176915E-5 0.0 0.0 0.0 2.5253169335884575E-5 9 5.050633867176915E-5 0.0 0.0 0.0 2.5253169335884575E-5 10-11 6.313292333971144E-5 0.0 0.0 5.050633867176915E-5 2.5253169335884575E-5 12-13 7.575950800765373E-5 0.0 0.0 7.575950800765373E-5 1.5151901601530746E-4 14-15 1.262658466794229E-4 0.0 0.0 1.6414560068324976E-4 2.020253546870766E-4 16-17 1.262658466794229E-4 0.0 0.0 1.893987700191343E-4 2.020253546870766E-4 18-19 1.262658466794229E-4 0.0 0.0 2.399051086909035E-4 2.020253546870766E-4 20-21 1.262658466794229E-4 0.0 0.0 3.1566461669855724E-4 2.525316933588458E-4 22-23 1.262658466794229E-4 0.0 0.0 3.5354437070238406E-4 2.7778486269473037E-4 24-25 1.262658466794229E-4 0.0 0.0 5.808228947253452E-4 2.7778486269473037E-4 26-27 1.262658466794229E-4 0.0 0.0 9.091140960918448E-4 2.7778486269473037E-4 28-29 2.020253546870766E-4 0.0 0.0 0.0031313929976496875 3.030380320306149E-4 30-31 2.272785240229612E-4 0.0 0.0 0.009962375303006466 3.030380320306149E-4 32-33 2.272785240229612E-4 0.0 0.0 0.023889498191746808 3.030380320306149E-4 34-35 2.399051086909035E-4 0.0 0.0 0.0459228884373061 3.282912013664995E-4 36-37 2.525316933588458E-4 0.0 0.0 0.08081014187483064 3.282912013664995E-4 38-39 2.525316933588458E-4 0.0 0.0 0.13064727155919886 3.5354437070238406E-4 40-41 2.525316933588458E-4 0.0 0.0 0.1887926939550731 3.7879754003826866E-4 42-43 2.525316933588458E-4 0.0 0.0 0.2487815977124669 3.7879754003826866E-4 44-45 3.030380320306149E-4 0.0 0.0 0.32365724479336466 3.9142412470621093E-4 46-47 3.409177860344418E-4 0.0 0.0 0.4151999836359463 4.1667729404209553E-4 48-49 4.040507093741532E-4 0.0 0.0 0.5151646544520454 4.293038787100378E-4 50-51 4.040507093741532E-4 0.0 0.0 0.6211648327394209 4.545570480459224E-4 52-53 4.040507093741532E-4 0.0 0.0 0.7295135557750336 4.545570480459224E-4 54-55 4.040507093741532E-4 0.0 0.0 0.8553500985757465 5.050633867176915E-4 56-57 4.040507093741532E-4 0.0 0.0 1.0074246843164434 5.176899713856338E-4 58-59 4.040507093741532E-4 0.0 0.0 1.1725677851884606 5.303165560535761E-4 60-61 4.293038787100378E-4 0.0 0.0 1.3517263950418936 5.68196310057403E-4 62-63 4.545570480459224E-4 0.0 0.0 1.5349381385737364 5.808228947253453E-4 64-65 4.545570480459224E-4 0.0 0.0 1.7339583661098428 5.808228947253453E-4 66-67 5.050633867176915E-4 0.0 0.0 1.9658329669519348 5.934494793932876E-4 68-69 5.050633867176915E-4 0.0 0.0 2.2288194724158368 6.060760640612298E-4 70-71 5.176899713856338E-4 0.0 0.0 2.5152661721927756 6.56582402732999E-4 72-73 5.429431407215184E-4 0.0 0.0 2.8135692349729124 7.449684954085949E-4 74-75 5.808228947253453E-4 0.0 0.0 3.1309636937709775 8.081014187483065E-4 76-77 5.808228947253453E-4 0.0 0.0 3.4698485996738806 8.838609267559602E-4 78-79 5.808228947253453E-4 0.0 0.0 3.862295477738195 8.838609267559602E-4 80-81 6.818355720688836E-4 0.0 0.0 4.284427456356841 8.838609267559602E-4 82-83 6.818355720688836E-4 0.0 0.0 4.723971495232581 9.091140960918448E-4 84-85 6.944621567368258E-4 0.0 0.0 5.177796201367762 9.722470194315563E-4 86-87 7.070887414047681E-4 0.0 0.0 5.657139235116855 0.0010227533581033254 88-89 7.070887414047681E-4 0.0 0.0 6.1840466133100875 0.0010858862814430367 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10995 0.0 20.956186 1 TACATGG 10600 0.0 20.893927 2 AGAGTAC 14685 0.0 20.511059 10-11 GAGTACT 10165 0.0 19.832472 12-13 GTATCAA 23870 0.0 19.782362 1 ACATGGG 11025 0.0 19.187084 3 AGTACTT 10545 0.0 19.072908 12-13 GTACTTT 10730 0.0 18.70079 14-15 TATACCG 210 2.0213498E-5 18.028133 5 CAACGCA 26125 0.0 17.878904 5 ATCAACG 26535 0.0 17.58482 3 CATGGGG 7530 0.0 17.534336 4 AACGCAG 26710 0.0 17.503935 6 TATCAAC 26795 0.0 17.467173 2 TCAACGC 26795 0.0 17.467173 4 GTATACG 250 4.648982E-6 17.06765 1 AGTACAT 8040 0.0 16.422083 2 GAGTACA 8910 0.0 16.33548 1 GGTATCA 19210 0.0 16.190031 1 ACGCAGA 28840 0.0 15.915824 7 >>END_MODULE