FastQCFastQC Report
Fri 27 May 2016
SRR938994_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938994_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3959899
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT116450.29407315691637587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89680.22647042260421288No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79640.20111624059098476No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52200.1318215439333175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.11861413637064987No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42010.10608856438005111No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG101450.022.402952
GTACATG110500.021.6017321
ACATGGG105600.020.1296353
GAGTACT106900.019.37586812-13
CATGGGG72450.019.3199214
AGAGTAC153350.019.16956310-11
AGTACTT112600.018.56359912-13
GTACTTT110500.018.48696114-15
GTATCAA246550.017.9524541
GGTATCA203500.016.5994991
CAACGCA271700.015.87435155
CGTATAC2406.210796E-515.816143
TATCAAC276350.015.7617682
ATCAACG273900.015.7468473
TCAACGC276600.015.6102914
AACGCAG278950.015.4957916
AGTACAT80900.015.4837852
GTGTAGC24100.015.4202251
GAGTACA90150.015.16803551
TACTTTT136350.015.03430214-15
Produced by FastQC (version 0.11.5)