##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938994_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3959899 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17474435585352 33.0 31.0 34.0 30.0 34.0 2 32.40617727876393 34.0 31.0 34.0 31.0 34.0 3 32.50980037622172 34.0 31.0 34.0 30.0 34.0 4 36.076089566930875 37.0 35.0 37.0 35.0 37.0 5 35.98586731631286 37.0 35.0 37.0 35.0 37.0 6 36.02503397182605 37.0 35.0 37.0 35.0 37.0 7 35.96664258356084 37.0 35.0 37.0 35.0 37.0 8 35.93254171381644 37.0 35.0 37.0 35.0 37.0 9 37.62267219441708 39.0 37.0 39.0 35.0 39.0 10-11 37.541295245156505 39.0 37.0 39.0 35.0 39.0 12-13 37.472132243776926 39.0 37.0 39.0 34.0 39.0 14-15 38.753843974303386 40.0 38.0 41.0 34.0 41.0 16-17 38.74506799289578 40.0 38.0 41.0 34.5 41.0 18-19 38.83115415317411 40.0 38.0 41.0 34.5 41.0 20-21 38.82070426543707 40.0 38.0 41.0 34.0 41.0 22-23 38.75992304349177 40.0 38.0 41.0 34.0 41.0 24-25 38.70359774327578 40.0 38.0 41.0 34.0 41.0 26-27 38.558897840576236 40.0 38.0 41.0 34.0 41.0 28-29 38.38473544401006 40.0 38.0 41.0 34.0 41.0 30-31 38.235546664195226 40.0 38.0 41.0 33.5 41.0 32-33 38.09719351427903 40.0 38.0 41.0 33.0 41.0 34-35 38.08376261616773 40.0 38.0 41.0 33.0 41.0 36-37 37.99800689361017 40.0 38.0 41.0 33.0 41.0 38-39 37.879589226896954 40.0 37.0 41.0 33.0 41.0 40-41 37.74051408886944 40.0 37.0 41.0 33.0 41.0 42-43 37.58341311230413 40.0 37.0 41.0 32.5 41.0 44-45 37.359447930363885 40.0 37.0 41.0 31.5 41.0 46-47 37.102982550817586 40.0 36.5 41.0 31.0 41.0 48-49 36.85048052993271 40.0 36.0 41.0 31.0 41.0 50-51 36.598302759742104 39.0 36.0 41.0 30.0 41.0 52-53 36.38003658679173 39.0 35.5 41.0 30.0 41.0 54-55 36.130905737747355 39.0 35.0 40.0 29.5 41.0 56-57 35.84015476152295 38.5 35.0 40.0 29.0 41.0 58-59 35.52927107484307 38.0 35.0 40.0 28.0 41.0 60-61 35.11917841843946 38.0 34.0 40.0 28.0 41.0 62-63 34.80607270538971 37.0 34.0 40.0 27.0 41.0 64-65 34.552888217603524 37.0 34.0 40.0 27.0 41.0 66-67 34.09548450604422 36.5 33.5 39.0 26.0 41.0 68-69 33.6750853999054 36.0 33.0 39.0 26.0 40.0 70-71 33.218599893583146 35.0 32.5 38.5 25.5 40.0 72-73 32.70232207942677 35.0 32.0 37.5 24.5 39.5 74-75 32.24410496328315 35.0 31.5 37.0 23.5 39.0 76-77 30.253652176482277 33.0 29.5 35.0 20.0 37.5 78-79 31.243882356595456 34.0 31.0 36.0 21.0 38.0 80-81 31.250443003723078 34.0 31.0 36.0 21.0 37.0 82-83 30.92854173805948 34.0 31.0 35.0 20.0 37.0 84-85 30.52431488782921 34.0 31.0 35.0 19.0 36.5 86-87 29.955292294071135 34.0 30.0 35.0 15.0 36.0 88-89 29.382425284079215 34.0 29.0 35.0 7.0 35.5 90-91 28.927157485582335 34.0 29.0 35.0 4.5 35.0 92-93 28.565193455691674 34.0 29.0 35.0 2.0 35.0 94-95 28.171288080832365 33.0 29.0 35.0 2.0 35.0 96-97 27.549275877995875 33.0 27.0 35.0 2.0 35.0 98-99 26.639291683954568 33.0 26.0 34.5 2.0 35.0 100-101 22.231238726038214 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11909838371002479 1101 2 0.0960774374610125 1101 3 0.093599028987569 1101 4 0.04661649614410379 1101 5 0.11197614501782027 1101 6 0.07352931686315856 1101 7 0.019161822448651833 1101 8 0.11707728314796384 1101 9 0.11082712972077502 1101 10-11 0.1368829727301204 1101 12-13 0.14834569192165503 1101 14-15 0.23582974495940334 1101 16-17 0.20867232673315783 1101 18-19 0.2148328719543855 1101 20-21 0.17424912229711964 1101 22-23 0.17584184897807376 1101 24-25 0.15960104890042004 1101 26-27 0.18073881515948642 1101 28-29 0.25650012359991337 1101 30-31 0.15151924221385826 1101 32-33 0.16473747469989064 1101 34-35 0.2057465748663887 1101 36-37 0.1514434001913898 1101 38-39 0.1763033902272042 1101 40-41 0.2432110719126186 1101 42-43 0.13840941289918618 1101 44-45 0.181853603269154 1101 46-47 0.19592630029694647 1101 48-49 0.044821063618194046 1101 50-51 -0.012068042522997757 1101 52-53 0.12079904150503751 1101 54-55 0.09733214001344237 1101 56-57 0.03860287082303415 1101 58-59 0.14363083653081077 1101 60-61 0.2885692991804021 1101 62-63 0.10709175997283182 1101 64-65 0.1501615616029781 1101 66-67 0.1834287411482194 1101 68-69 0.20059800307590336 1101 70-71 0.10431339236689041 1101 72-73 0.0011387362868546802 1101 74-75 -0.017746536464748885 1101 76-77 -0.1491384179808044 1101 78-79 0.12627203227201633 1101 80-81 0.2887592967179877 1101 82-83 0.24414864111047407 1101 84-85 0.23067053446229835 1101 86-87 0.35169563082773436 1101 88-89 0.39311688903948294 1101 90-91 0.47456851600218997 1101 92-93 0.5673985308058747 1101 94-95 0.6042195562684043 1101 96-97 0.5088428208590727 1101 98-99 0.3726474583713788 1101 100-101 0.7215758285079943 1102 1 0.113681185745925 1102 2 0.11423420145779772 1102 3 0.14997070503007137 1102 4 0.12547353695131847 1102 5 0.1311970040230861 1102 6 0.14713438874457552 1102 7 0.09434967850722842 1102 8 0.11678097097723139 1102 9 0.16236258824385175 1102 10-11 0.21491826159520144 1102 12-13 0.21140564370306691 1102 14-15 0.27759743850346297 1102 16-17 0.2924706301531117 1102 18-19 0.20623771167221605 1102 20-21 0.2778889472814896 1102 22-23 0.2495948626400164 1102 24-25 0.20728680655735587 1102 26-27 0.2158983115430928 1102 28-29 0.34891441058982053 1102 30-31 0.29585137901178626 1102 32-33 0.28938646121654443 1102 34-35 0.2886911234867995 1102 36-37 0.2762139689921739 1102 38-39 0.29159832727516033 1102 40-41 0.22871209879001952 1102 42-43 0.13508597330636007 1102 44-45 0.18963354790707143 1102 46-47 0.3266543295852813 1102 48-49 0.24124463715154576 1102 50-51 0.3162672016952399 1102 52-53 0.3092604755616506 1102 54-55 0.3228995299589741 1102 56-57 0.27005267884161555 1102 58-59 0.22909150266171707 1102 60-61 0.1348255363444295 1102 62-63 0.2065413179690765 1102 64-65 0.22960376179941733 1102 66-67 0.18411148067853844 1102 68-69 0.2908271663940596 1102 70-71 0.1769272686058443 1102 72-73 0.16600874253741438 1102 74-75 0.2814518205314087 1102 76-77 0.1363063538738274 1102 78-79 0.06505395922977897 1102 80-81 0.3176247311317759 1102 82-83 0.34918198337317463 1102 84-85 0.3147940812075447 1102 86-87 0.4285248120046248 1102 88-89 0.5748426145506862 1102 90-91 0.5275294232260457 1102 92-93 0.41518172573399426 1102 94-95 0.4533846245780744 1102 96-97 0.36765396471120226 1102 98-99 0.6073935249844702 1102 100-101 1.0498400663863805 1103 1 -0.02356872231816709 1103 2 -0.032316048082520865 1103 3 5.682358942706855E-4 1103 4 0.03717303787068005 1103 5 0.04883840144888296 1103 6 -0.01279143211174727 1103 7 0.09869998058235296 1103 8 0.0448847797505465 1103 9 0.03952024519158215 1103 10-11 0.08115424793615489 1103 12-13 0.024497001727759482 1103 14-15 0.07780095833535938 1103 16-17 0.06855163882135429 1103 18-19 0.04097887269138312 1103 20-21 0.08703441615462282 1103 22-23 0.08035924404268968 1103 24-25 0.05635158197106449 1103 26-27 -0.008846001564336348 1103 28-29 0.08659583953649985 1103 30-31 0.11538098237401329 1103 32-33 0.12637306484144517 1103 34-35 0.013664068062581691 1103 36-37 -0.03151936434115754 1103 38-39 0.08091169738285231 1103 40-41 0.055762794876173416 1103 42-43 0.04596740225303364 1103 44-45 0.07421805013979821 1103 46-47 0.13521813310511277 1103 48-49 0.06524074957602721 1103 50-51 -0.017521923600270384 1103 52-53 0.06672756250674894 1103 54-55 0.1533813791840828 1103 56-57 0.08595097146046982 1103 58-59 -0.09260064338504037 1103 60-61 0.004929345117744788 1103 62-63 0.10829314355353148 1103 64-65 0.0602453168296293 1103 66-67 -0.02176663857547112 1103 68-69 -0.015341785536591601 1103 70-71 -0.1033250035244464 1103 72-73 -0.0851353604308045 1103 74-75 0.0010044341379185084 1103 76-77 -0.08642230386192296 1103 78-79 0.09087260908530936 1103 80-81 0.03699641487225236 1103 82-83 0.1416324167853098 1103 84-85 0.04195909302787726 1103 86-87 -0.05522354628273973 1103 88-89 0.13607318980679395 1103 90-91 0.2571076738170639 1103 92-93 0.2541804123638869 1103 94-95 0.12877658675301618 1103 96-97 -0.028069176870094026 1103 98-99 0.06630826099707932 1103 100-101 0.7649066279834358 1104 1 -0.021849514622694244 1104 2 0.0023239328953934546 1104 3 -0.008968947418587447 1104 4 -0.010713930136617478 1104 5 0.044743923454369394 1104 6 -0.06485255175042681 1104 7 -0.029330574257386388 1104 8 0.03117717950803467 1104 9 -0.026013188480739302 1104 10-11 -0.04831354461859405 1104 12-13 -0.024022686997305698 1104 14-15 0.011785748140120234 1104 16-17 0.04317247322841666 1104 18-19 -0.012842225136637353 1104 20-21 -0.05372369094915541 1104 22-23 0.00527680050203827 1104 24-25 0.022282699132397 1104 26-27 0.05296455693540736 1104 28-29 0.003665542764657914 1104 30-31 -0.06813261256272796 1104 32-33 0.038915687124919884 1104 34-35 0.014364635861738861 1104 36-37 0.004288955827355778 1104 38-39 0.16872418298869007 1104 40-41 0.22361678546722175 1104 42-43 0.15734990168972018 1104 44-45 0.11416877697499928 1104 46-47 0.17708445070165624 1104 48-49 0.15426327854174104 1104 50-51 0.016348086371380077 1104 52-53 0.021946310211468756 1104 54-55 0.14678620715749702 1104 56-57 0.20934894003170967 1104 58-59 0.17997407243527874 1104 60-61 0.30335971222647373 1104 62-63 0.3731734401386433 1104 64-65 0.4209238249905738 1104 66-67 0.4122863095358298 1104 68-69 0.37863843548305454 1104 70-71 0.28251294527777304 1104 72-73 0.26648836339050064 1104 74-75 0.1387228052602083 1104 76-77 -0.09907858556798743 1104 78-79 0.11231104190487073 1104 80-81 0.20100335145826165 1104 82-83 0.27130499548481524 1104 84-85 0.11067106909590763 1104 86-87 -0.023244540286050608 1104 88-89 0.19402829122261878 1104 90-91 0.3032433779390544 1104 92-93 0.22651716543909828 1104 94-95 0.2819367046202039 1104 96-97 0.03794043127254909 1104 98-99 0.13960458343628446 1104 100-101 0.8665113670709488 1105 1 -0.014533493625698668 1105 2 0.04775488134448125 1105 3 0.0718158076139801 1105 4 0.029575176577040452 1105 5 0.05584509398789095 1105 6 0.02318699411842573 1105 7 0.018028208853181127 1105 8 0.02879388195671595 1105 9 -0.09170433595564731 1105 10-11 0.004198268462744181 1105 12-13 0.031997248132626055 1105 14-15 0.09604739987354094 1105 16-17 0.1412372882353452 1105 18-19 0.011158065350642232 1105 20-21 0.05923649642734574 1105 22-23 0.055332694213539924 1105 24-25 0.021324681053023653 1105 26-27 0.04320441507130113 1105 28-29 0.06772910713029745 1105 30-31 0.058676033488559654 1105 32-33 0.13738680459366037 1105 34-35 0.12702952163256498 1105 36-37 0.15377551075541618 1105 38-39 0.08835444367999656 1105 40-41 0.09583150818138364 1105 42-43 0.036586462618750204 1105 44-45 -0.005036174815906236 1105 46-47 0.2156505352681677 1105 48-49 0.1109048998350346 1105 50-51 0.09692250257943158 1105 52-53 0.15098426301121748 1105 54-55 0.2248662910543473 1105 56-57 0.18196559840551885 1105 58-59 0.005443635892035559 1105 60-61 -0.03206463448568542 1105 62-63 -0.08084091226527335 1105 64-65 -0.09014327228076269 1105 66-67 0.07256147638632626 1105 68-69 0.04839464858137177 1105 70-71 0.1569304620130012 1105 72-73 0.12993361162488526 1105 74-75 0.08339684585546792 1105 76-77 -0.03499556045358432 1105 78-79 0.10454586411431777 1105 80-81 0.0892820407720869 1105 82-83 0.14281333370367477 1105 84-85 0.09251924216556517 1105 86-87 -0.0020194407209288556 1105 88-89 0.1521225184050934 1105 90-91 0.28085853302087926 1105 92-93 0.13275198927592768 1105 94-95 0.13250046284745665 1105 96-97 0.2194330015383983 1105 98-99 0.43185217379552654 1105 100-101 0.8442695281427532 1106 1 0.012287968890838386 1106 2 -0.015781420330483797 1106 3 -0.03569164508983391 1106 4 -0.020799159177492754 1106 5 0.0032982715804692475 1106 6 -6.042888847517247E-4 1106 7 0.01185814030921506 1106 8 0.04066600009679178 1106 9 -0.0151172336129477 1106 10-11 0.0792011666996899 1106 12-13 0.07958467761879007 1106 14-15 0.027460953405835653 1106 16-17 0.011929965541945364 1106 18-19 -0.09324046578581857 1106 20-21 -0.03021636275619244 1106 22-23 0.1204376170079442 1106 24-25 0.04386035914385644 1106 26-27 0.08965226837762685 1106 28-29 0.15255415022668473 1106 30-31 0.08539622313649176 1106 32-33 0.14536830431350012 1106 34-35 0.15601691999245304 1106 36-37 0.15309009095174986 1106 38-39 0.15719339407885968 1106 40-41 0.24925646329840845 1106 42-43 0.2905827872837463 1106 44-45 0.18457852906499994 1106 46-47 0.2030009551419738 1106 48-49 0.14622901536325372 1106 50-51 0.07581040142116535 1106 52-53 0.18061450091279596 1106 54-55 0.18501662485448378 1106 56-57 0.15626425814959788 1106 58-59 0.24282257494674298 1106 60-61 0.2343522235660842 1106 62-63 0.19168910830823904 1106 64-65 0.04706035652363738 1106 66-67 0.15481172048669123 1106 68-69 0.14361291493808181 1106 70-71 0.13052891300460345 1106 72-73 0.16181888299647795 1106 74-75 0.2645900870610447 1106 76-77 0.06703571324347024 1106 78-79 0.20538187975050448 1106 80-81 0.3641101713510295 1106 82-83 0.49468715637762983 1106 84-85 0.3887877091623224 1106 86-87 0.3225090531831114 1106 88-89 0.3710953038278362 1106 90-91 0.6165632704339963 1106 92-93 0.547360896682946 1106 94-95 0.7615509144033581 1106 96-97 0.6069119297677297 1106 98-99 0.5532115448542889 1106 100-101 0.8994830263041607 1107 1 -0.09130112371112631 1107 2 -0.054727103665833 1107 3 -0.038070623884877364 1107 4 -0.008583732145460488 1107 5 -0.07218870419341528 1107 6 -0.05956789692115905 1107 7 -0.12499035038222672 1107 8 -0.0023259709889487112 1107 9 -0.04986900322526111 1107 10-11 0.022139503841778208 1107 12-13 0.057159709469054576 1107 14-15 0.04295486904132417 1107 16-17 0.044696545693931 1107 18-19 -0.011975118644727445 1107 20-21 0.02056084803771796 1107 22-23 0.06748423301981887 1107 24-25 0.04434715153143287 1107 26-27 0.05927776604220014 1107 28-29 0.08547523988713124 1107 30-31 -0.011896399049220463 1107 32-33 0.031517143517504564 1107 34-35 -0.032081754412757846 1107 36-37 0.019334573971356406 1107 38-39 -0.07806162684159546 1107 40-41 -0.11828475735749322 1107 42-43 -0.14004571162863755 1107 44-45 -0.20415484532940553 1107 46-47 -0.349826187026153 1107 48-49 -0.20953415458946978 1107 50-51 -0.07593930692911499 1107 52-53 -0.03892608234471595 1107 54-55 -0.14907644081169735 1107 56-57 -0.0926593952513386 1107 58-59 -0.19982293356905956 1107 60-61 -0.3873829435604037 1107 62-63 -0.22034657395037271 1107 64-65 -0.17108017056526847 1107 66-67 -0.007699718233865838 1107 68-69 -0.1364672851311326 1107 70-71 -0.12657403141965062 1107 72-73 2.822407105824709E-4 1107 74-75 -0.0861541272976396 1107 76-77 -0.48876595473353035 1107 78-79 -0.20191883370080177 1107 80-81 0.08278880624435914 1107 82-83 0.050925512984864696 1107 84-85 -0.06063523309273933 1107 86-87 -0.04808407575134055 1107 88-89 0.14259702019262477 1107 90-91 0.29543109711507753 1107 92-93 0.4347123570535594 1107 94-95 0.31147557538103143 1107 96-97 0.25421985816009496 1107 98-99 -0.032893432276129175 1107 100-101 0.6064593883338674 1108 1 0.07697726929684023 1108 2 0.02379242600088105 1108 3 0.018529103637554556 1108 4 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67.0 6 138.0 7 396.0 8 955.0 9 1789.0 10 3114.0 11 4883.0 12 7231.0 13 9787.0 14 11623.0 15 15256.0 16 19705.0 17 26880.0 18 26737.0 19 20160.0 20 19267.0 21 20586.0 22 22739.0 23 25465.0 24 29827.0 25 36037.0 26 43416.0 27 52776.0 28 64872.0 29 78934.0 30 96013.0 31 118076.0 32 144863.0 33 185505.0 34 251190.0 35 358865.0 36 576081.0 37 919516.0 38 712596.0 39 54522.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.883723631008316 19.77269304127907 14.81874321393235 23.52484011378026 2 15.49004153893824 20.918361806702645 39.66366313888309 23.927933515476028 3 19.309684413668126 24.176020651031756 30.22114957982514 26.293145355474973 4 13.233645605607618 17.08722873992493 37.10268873019236 32.576436924275086 5 13.681518792731703 37.26409019258654 34.79692071632437 14.257470298357374 6 31.930511359001834 36.60737306683832 17.13389659685765 14.328218977302198 7 28.085009238872 32.56916401150635 21.162736726366003 18.183090023255644 8 25.367818724669494 35.87129368703596 19.845102109927552 18.915785478367 9 25.59532452721648 17.1707409709187 20.728205441603436 36.50572906026139 10-11 24.833512167860846 26.44432345370425 27.6603771964891 21.061787181945803 12-13 25.612282030425526 23.775833676565995 27.985322352918594 22.626561940089886 14-15 22.49543796311682 25.409560554505294 26.262862216637277 25.832139265740604 16-17 22.027267040574532 28.374508403078035 27.012179153537847 22.58604540280959 18-19 22.01072552608034 27.56675107117631 28.585653320955913 21.836870081787442 20-21 22.904409935707957 26.609643326761617 28.49811320945307 21.987833528077356 22-23 22.42693563648972 26.47910969446443 28.53450302646608 22.559451642579774 24-25 22.543302240789476 26.521156221408678 28.576208635624294 22.359332902177556 26-27 22.368537682400486 26.565967970395203 28.76969841907584 22.29579592812847 28-29 22.17891163385733 27.102812470722103 28.561258759377452 22.157017136043116 30-31 22.507467993501855 26.625477063935215 28.332831216149707 22.534223726413224 32-33 22.366340656668264 26.72349724071245 28.411621104477664 22.49854099814162 34-35 22.671929050998006 26.794016468500192 28.32433288331083 22.20972159719097 36-37 22.663364821330617 26.48215579133671 28.59876896780587 22.2557104195268 38-39 22.35697343192919 26.91385591563157 28.435009839254505 22.294160813184742 40-41 22.67868078696192 26.834436718155153 28.20355145544518 22.283331039437748 42-43 22.375742858419954 26.887061782031896 28.021643623419234 22.715551736128912 44-45 22.58292732386285 26.803443435071188 28.06133413537723 22.55229510568873 46-47 22.881341593499613 27.016445919682575 27.751908639840316 22.35030384697749 48-49 22.659649126351333 26.96766347120511 27.82694304155649 22.545744360887067 50-51 22.35013583122205 27.25966901753905 27.910888657540546 22.479306493698353 52-53 22.769873676071033 27.2805695296774 27.29386532333274 22.65569147091883 54-55 22.663785111690977 27.099037121906395 27.41702503018385 22.820152736218777 56-57 22.589439781166135 27.19309255109789 27.4706122555146 22.74685541222137 58-59 22.893025171535722 26.95932550643143 27.470640279230015 22.677009042802837 60-61 22.606153042308684 27.137237650207517 27.72802304957039 22.52858625791341 62-63 22.360494547967004 27.075425408577342 27.88264296639889 22.681437077056763 64-65 22.26461960540298 27.35361454667405 27.59107075755678 22.790695090366192 66-67 22.467313401306594 27.176264504526255 27.278730726974803 23.077691367192347 68-69 22.333477026406946 27.060436195603394 27.618048658161754 22.988038119827902 70-71 22.60322801162353 26.81530513783306 27.46727883716226 23.11418801338115 72-73 22.504286094165533 26.806491781734838 27.57425126246907 23.114970861630564 74-75 22.153708970860116 27.482973681904515 27.239899300461957 23.123418046773413 76-77 22.315808158074628 26.857690027653096 27.41445624993758 23.4120455643347 78-79 22.45338575896583 26.987255975221565 27.369084281275136 23.19027398453747 80-81 22.441014278394476 26.871872237145446 27.2971103555924 23.390003128867683 82-83 22.42196076213055 26.737891547233904 27.631992634155566 23.208155056479978 84-85 22.299066213557467 26.92441145594875 27.3914435696466 23.385078760847183 86-87 22.09495259350701 26.83638592022669 27.51764283816541 23.55101864810089 88-89 22.189431400901423 26.75045299993316 27.274901806152936 23.78521379301248 90-91 22.130127404892942 26.62280425572405 27.32793586386224 23.91913247552077 92-93 22.25560550913041 26.716603125483758 27.29044351888773 23.737347846498107 94-95 22.115298900300235 26.59752180548039 27.377112396048485 23.910066898170886 96-97 21.79636918473833 26.426115727690874 27.68735280495732 24.090162282613473 98-99 21.821377262399874 26.602168388638198 27.30692121187939 24.269533137082536 100-101 21.908951775307926 25.94278806446794 27.830643296828534 24.317616863395592 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 570.0 1 414.0 2 304.5 3 358.5 4 324.5 5 339.5 6 604.5 7 1182.5 8 1915.5 9 2362.5 10 2298.0 11 2186.5 12 2361.0 13 2704.0 14 3032.0 15 3247.5 16 3459.0 17 3745.5 18 4147.5 19 4636.5 20 5260.0 21 6488.5 22 8067.0 23 10171.0 24 12726.5 25 16055.5 26 21263.5 27 27775.5 28 33687.0 29 39087.0 30 44072.0 31 51259.0 32 58854.0 33 65213.0 34 72525.5 35 80730.5 36 90692.5 37 98873.5 38 104578.0 39 109884.0 40 115816.5 41 120772.5 42 123735.0 43 127629.5 44 134301.5 45 140165.0 46 142794.0 47 153870.0 48 172158.5 49 170907.0 50 156408.5 51 149071.5 52 146213.5 53 143630.0 54 137858.0 55 127040.0 56 115584.0 57 104617.5 58 95591.5 59 87279.0 60 73877.5 61 59503.5 62 46573.0 63 34356.0 64 24609.5 65 17637.0 66 12163.5 67 8866.0 68 6626.0 69 4797.5 70 3370.0 71 1991.0 72 1131.0 73 723.5 74 467.5 75 271.5 76 147.5 77 63.5 78 33.5 79 17.0 80 10.5 81 14.0 82 13.0 83 7.0 84 3.5 85 1.5 86 3.0 87 2.5 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40801545695988717 2 0.0 3 0.0 4 0.0 5 4.293038787100378E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011363926201148058 16-17 0.0025253169335884575 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002449557425580804 36-37 0.007449684954085951 38-39 0.012714970760617885 40-41 0.002878861304290842 42-43 6.56582402732999E-4 44-45 4.0405070937415326E-4 46-47 2.777848626947303E-4 48-49 3.030380320306149E-4 50-51 5.429431407215184E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.3990510869090347E-4 60-61 0.0021591459782181314 62-63 0.0 64-65 2.777848626947303E-4 66-67 0.0014520572368133633 68-69 0.0017677218535119203 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.126455245449442 78-79 1.0101267734353831E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.15044575631853238 88-89 0.44368555864682413 90-91 0.34201629890055274 92-93 0.0 94-95 0.0 96-97 4.293038787100378E-4 98-99 0.0 100-101 2.146519393550189E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3959899.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.41575747752112 #Duplication Level Percentage of deduplicated Percentage of total 1 68.0086186492884 30.20654312308013 2 15.325291049224216 13.613688210295358 3 6.17933069428808 8.233789604727045 4 3.2179660880816017 5.717136053564791 5 1.8580088260320524 4.126243470406668 6 1.2332659792483753 3.2865865583744056 7 0.8127484966766703 2.52691880830276 8 0.5868368194562901 2.0851841481480338 9 0.42925496396647567 1.7159115938001346 >10 2.15534747898398 16.756744431780515 >50 0.12622191336378347 3.8375845107251907 >100 0.06284677143057647 4.994445375885758 >500 0.0026710225416477556 0.8003604410218588 >1k 0.0013639263582233302 1.2457735633200169 >5k 1.7049079477791552E-4 0.5591517602355424 >10k+ 5.683026492597184E-5 0.29393834633183863 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11645 0.29407315691637587 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8968 0.22647042260421288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7964 0.20111624059098476 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5220 0.1318215439333175 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4697 0.11861413637064987 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4201 0.10608856438005111 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.050633867176915E-5 2 0.0 0.0 0.0 0.0 5.050633867176915E-5 3 0.0 0.0 0.0 0.0 5.050633867176915E-5 4 0.0 0.0 0.0 0.0 5.050633867176915E-5 5 0.0 0.0 0.0 0.0 5.050633867176915E-5 6 0.0 0.0 0.0 0.0 7.575950800765373E-5 7 0.0 0.0 0.0 0.0 7.575950800765373E-5 8 0.0 0.0 0.0 0.0 7.575950800765373E-5 9 0.0 0.0 0.0 0.0 7.575950800765373E-5 10-11 0.0 0.0 0.0 5.050633867176915E-5 7.575950800765373E-5 12-13 0.0 0.0 0.0 7.575950800765373E-5 1.6414560068324976E-4 14-15 1.2626584667942288E-5 0.0 0.0 1.3889243134736516E-4 2.146519393550189E-4 16-17 2.5253169335884575E-5 0.0 0.0 1.6414560068324976E-4 2.525316933588458E-4 18-19 5.050633867176915E-5 0.0 0.0 2.146519393550189E-4 3.030380320306149E-4 20-21 7.575950800765373E-5 0.0 0.0 2.7778486269473037E-4 3.7879754003826866E-4 22-23 7.575950800765373E-5 0.0 0.0 3.0303803203061497E-4 4.040507093741532E-4 24-25 7.575950800765373E-5 0.0 0.0 5.303165560535761E-4 4.040507093741532E-4 26-27 7.575950800765373E-5 0.0 0.0 8.964875114239025E-4 4.293038787100378E-4 28-29 7.575950800765373E-5 0.0 0.0 0.0031945259209893986 4.293038787100378E-4 30-31 1.010126773435383E-4 0.0 0.0 0.01020228041169737 4.293038787100378E-4 32-33 1.010126773435383E-4 0.0 0.0 0.02421778939311331 4.293038787100378E-4 34-35 1.1363926201148059E-4 0.0 0.0 0.04650371133203145 4.545570480459224E-4 36-37 1.5151901601530746E-4 0.0 0.0 0.08218643960363636 4.545570480459224E-4 38-39 1.5151901601530746E-4 0.0 0.0 0.13252863267472226 4.671836327138647E-4 40-41 1.5151901601530746E-4 0.0 0.0 0.19122962479598596 4.7981021738180695E-4 42-43 1.8939877001913433E-4 0.0 0.0 0.2521528958188075 5.050633867176915E-4 44-45 2.272785240229612E-4 0.0 0.0 0.32800078991913684 5.303165560535761E-4 46-47 2.777848626947303E-4 0.0 0.0 0.42080618722851265 5.303165560535761E-4 48-49 3.282912013664995E-4 0.0 0.0 0.5213895606933409 5.555697253894607E-4 50-51 3.282912013664995E-4 0.0 0.0 0.6280589479681173 5.808228947253453E-4 52-53 3.282912013664995E-4 0.0 0.0 0.7372031458378105 6.060760640612298E-4 54-55 3.282912013664995E-4 0.0 0.0 0.8640119356579549 6.060760640612298E-4 56-57 3.282912013664995E-4 0.0 0.0 1.0172734203574385 6.060760640612298E-4 58-59 3.282912013664995E-4 0.0 0.0 1.1835276606802345 6.187026487291721E-4 60-61 3.282912013664995E-4 0.0 0.0 1.3638479163231183 6.56582402732999E-4 62-63 3.282912013664995E-4 0.0 0.0 1.5479308941970489 6.56582402732999E-4 64-65 3.282912013664995E-4 0.0 0.0 1.748327419461961 6.692089874009413E-4 66-67 3.282912013664995E-4 0.0 0.0 1.981565691448191 6.818355720688836E-4 68-69 3.282912013664995E-4 0.0 0.0 2.2455370705161926 6.818355720688836E-4 70-71 3.5354437070238406E-4 0.0 0.0 2.5338525048239866 7.070887414047681E-4 72-73 3.6617095537032633E-4 0.0 0.0 2.8342389540743333 7.323419107406527E-4 74-75 3.7879754003826866E-4 0.0 0.0 3.154045090543976 7.702216647444796E-4 76-77 3.7879754003826866E-4 0.0 0.0 3.4968947440326126 8.081014187483064E-4 78-79 3.9142412470621093E-4 0.0 0.0 3.891627033921825 8.081014187483064E-4 80-81 4.293038787100378E-4 0.0 0.0 4.316915658707457 8.459811727521333E-4 82-83 4.545570480459224E-4 0.0 0.0 4.758606216976746 9.59620434763614E-4 84-85 4.671836327138647E-4 0.0 0.0 5.215347664170222 9.848736040994985E-4 86-87 4.7981021738180695E-4 0.0 0.0 5.697380160453587 0.001010126773435383 88-89 4.7981021738180695E-4 0.0 0.0 6.226434058040369 0.0010353799427712676 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 10145 0.0 22.40295 2 GTACATG 11050 0.0 21.601732 1 ACATGGG 10560 0.0 20.129635 3 GAGTACT 10690 0.0 19.375868 12-13 CATGGGG 7245 0.0 19.319921 4 AGAGTAC 15335 0.0 19.169563 10-11 AGTACTT 11260 0.0 18.563599 12-13 GTACTTT 11050 0.0 18.486961 14-15 GTATCAA 24655 0.0 17.952454 1 GGTATCA 20350 0.0 16.599499 1 CAACGCA 27170 0.0 15.8743515 5 CGTATAC 240 6.210796E-5 15.81614 3 TATCAAC 27635 0.0 15.761768 2 ATCAACG 27390 0.0 15.746847 3 TCAACGC 27660 0.0 15.610291 4 AACGCAG 27895 0.0 15.495791 6 AGTACAT 8090 0.0 15.483785 2 GTGTAGC 2410 0.0 15.420225 1 GAGTACA 9015 0.0 15.1680355 1 TACTTTT 13635 0.0 15.034302 14-15 >>END_MODULE