FastQCFastQC Report
Fri 27 May 2016
SRR938993_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938993_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4042555
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104840.2593409366106336No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85580.21169780002993155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52190.12910152119142473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126850.027.240051
GTATCAA178150.026.8560221
GTACATG133550.023.669951
TACATGG135750.022.6436212
ATCAACG212550.022.3501723
TATCAAC214450.022.2404942
CAACGCA216150.021.9779285
TCAACGC216900.021.9456024
ACATGGG137250.021.636963
AACGCAG221300.021.4336516
GAGTACT101500.020.21825412-13
ACGCAGA242250.019.1503857
AGTACTT109150.018.88795912-13
CATGGGG74050.018.7406044
CGCAGAG243750.018.6635388
GTACTTT114250.017.94212214-15
GCAGAGT250650.017.4698529
GTGTAGC21450.017.259741
AGAGTAC200550.017.10137210-11
CATGGGA74350.015.9257564
Produced by FastQC (version 0.11.5)