##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938993_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4042555 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.691152748694822 33.0 31.0 34.0 30.0 34.0 2 31.781838960756254 33.0 31.0 34.0 30.0 34.0 3 31.831816017345467 34.0 31.0 34.0 30.0 34.0 4 35.36515767874525 37.0 35.0 37.0 33.0 37.0 5 35.40047272084115 37.0 35.0 37.0 33.0 37.0 6 35.47577237662815 37.0 35.0 37.0 33.0 37.0 7 35.39109721450914 37.0 35.0 37.0 33.0 37.0 8 35.372659617494385 37.0 35.0 37.0 33.0 37.0 9 36.96665376228648 39.0 37.0 39.0 33.0 39.0 10-11 36.8449605509387 39.0 37.0 39.0 33.0 39.0 12-13 36.726697719635226 39.0 37.0 39.0 32.0 39.0 14-15 37.92676079855438 40.0 38.0 41.0 33.0 41.0 16-17 37.83189517520479 40.0 38.0 41.0 32.0 41.0 18-19 37.72268491090412 40.0 38.0 41.0 32.0 41.0 20-21 37.84082480015732 40.0 38.0 41.0 32.0 41.0 22-23 37.778279825506395 40.0 38.0 41.0 32.0 41.0 24-25 37.69928498189883 40.0 38.0 41.0 32.0 41.0 26-27 37.58351314948096 40.0 37.0 41.0 32.0 41.0 28-29 37.45273743956483 40.0 37.0 41.0 31.5 41.0 30-31 37.26081364384653 40.0 37.0 41.0 31.0 41.0 32-33 37.08055895343415 40.0 36.5 41.0 30.5 41.0 34-35 36.76267768774946 40.0 36.0 41.0 30.0 41.0 36-37 36.5019146307224 39.0 36.0 41.0 30.0 41.0 38-39 36.26741095668457 39.0 35.5 41.0 29.5 41.0 40-41 36.11870030216039 39.0 35.0 41.0 29.0 41.0 42-43 35.922769387182115 39.0 35.0 41.0 28.5 41.0 44-45 35.885008243548945 39.0 35.0 41.0 28.0 41.0 46-47 35.70528910552856 39.0 35.0 41.0 27.0 41.0 48-49 35.49536555470488 39.0 35.0 41.0 26.0 41.0 50-51 34.40148037565352 38.0 34.0 40.0 24.5 40.5 52-53 34.50521019503754 38.0 34.0 39.5 24.5 40.5 54-55 34.99848833225522 39.0 35.0 40.0 25.5 41.0 56-57 34.90990809030427 39.0 34.5 40.0 25.0 41.0 58-59 34.69988064479024 38.0 34.0 40.0 24.5 41.0 60-61 34.39719533809682 38.0 34.0 40.0 23.5 41.0 62-63 34.01364397021191 37.5 34.0 40.0 22.5 41.0 64-65 33.62498135461361 37.0 33.0 40.0 22.0 41.0 66-67 33.22043027243909 36.5 33.0 39.0 21.5 41.0 68-69 32.785073672467036 36.0 32.0 39.0 20.0 41.0 70-71 32.34803768903577 35.5 32.0 39.0 18.5 40.0 72-73 31.90353835631179 35.0 31.0 38.0 17.5 40.0 74-75 31.429431634201638 35.0 31.0 37.0 13.5 39.0 76-77 30.99153295378789 35.0 31.0 37.0 9.5 39.0 78-79 30.585977679957352 35.0 30.5 36.0 7.0 39.0 80-81 30.128995523870422 34.0 30.0 36.0 4.0 37.5 82-83 29.66026362040838 34.0 29.5 35.0 2.0 37.0 84-85 29.242260278462506 34.0 29.0 35.0 2.0 36.5 86-87 28.781473845130122 34.0 29.0 35.0 2.0 36.0 88-89 28.30746903876385 34.0 28.5 35.0 2.0 36.0 90-91 27.84562807432428 34.0 27.0 35.0 2.0 35.0 92-93 27.339320058725235 33.5 26.0 35.0 2.0 35.0 94-95 26.795833575548137 33.0 25.0 35.0 2.0 35.0 96-97 26.134265953091546 33.0 24.5 35.0 2.0 35.0 98-99 25.246415076603782 32.5 20.0 35.0 2.0 35.0 100-101 21.980297114077608 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16824338022995278 1101 2 0.33190979072788096 1101 3 0.3902134384788276 1101 4 0.2729162813788264 1101 5 0.09964335601234353 1101 6 0.2954116103508042 1101 7 0.28273449529046246 1101 8 0.2289608304422046 1101 9 0.4212896417153118 1101 10-11 0.28135664468903343 1101 12-13 0.3638164379025568 1101 14-15 0.5408377792951882 1101 16-17 0.4495954316985049 1101 18-19 0.5111036847575932 1101 20-21 0.5092102677377355 1101 22-23 0.6135240381276006 1101 24-25 0.5992766321241234 1101 26-27 0.6208921920856767 1101 28-29 0.6049027032759966 1101 30-31 0.5179820160236943 1101 32-33 0.526814539074671 1101 34-35 0.4957621420638887 1101 36-37 0.49768270453535735 1101 38-39 0.5762902458903909 1101 40-41 0.4130497388032168 1101 42-43 0.44387022170874957 1101 44-45 0.6705005674485847 1101 46-47 0.567663107021545 1101 48-49 0.590681704769203 1101 50-51 0.4138067892592048 1101 52-53 0.5998304549503857 1101 54-55 0.5711119397585165 1101 56-57 0.6718477424694882 1101 58-59 0.6562145817858891 1101 60-61 0.6812122141030841 1101 62-63 0.6334659188312415 1101 64-65 0.47894451048922093 1101 66-67 0.494978871352707 1101 68-69 0.4982544539896523 1101 70-71 0.4368480948814053 1101 72-73 0.41411884855431325 1101 74-75 0.18898464872545162 1101 76-77 0.23385471921817214 1101 78-79 0.13394710918603892 1101 80-81 0.1860699534697723 1101 82-83 0.23142075553729669 1101 84-85 0.14970867233354213 1101 86-87 0.1533547503077699 1101 88-89 0.11921521223149512 1101 90-91 0.17951108495847734 1101 92-93 0.20836571443599539 1101 94-95 -0.021386926697463338 1101 96-97 -0.2541720892927195 1101 98-99 -0.3270719272290741 1101 100-101 0.04419300918283042 1102 1 0.2795341275054426 1102 2 0.26737321274251613 1102 3 0.3827083662097941 1102 4 0.17863608353711413 1102 5 0.3109492047267892 1102 6 0.32134679588919823 1102 7 0.39733934406676497 1102 8 0.3315522487959299 1102 9 0.5680213374354608 1102 10-11 0.5081947234031148 1102 12-13 0.4610596680789172 1102 14-15 0.5399295498967618 1102 16-17 0.6609375338673829 1102 18-19 0.604078436780398 1102 20-21 0.4976513091912622 1102 22-23 0.5925367883425068 1102 24-25 0.601680575471292 1102 26-27 0.5521624288353806 1102 28-29 0.4697942222250191 1102 30-31 0.5215203888943662 1102 32-33 0.6306161441704674 1102 34-35 0.6197914602860664 1102 36-37 0.551878332890233 1102 38-39 0.5780636648718911 1102 40-41 0.49847791460225466 1102 42-43 0.4947129591524444 1102 44-45 0.6265757391827975 1102 46-47 0.7289069712583185 1102 48-49 0.6933606314612177 1102 50-51 0.5644048891067186 1102 52-53 0.5453750518426901 1102 54-55 0.7364342283251588 1102 56-57 0.6777134085265288 1102 58-59 0.6169375535319048 1102 60-61 0.7660821938568745 1102 62-63 0.7207454138916987 1102 64-65 0.6206830161439711 1102 66-67 0.512620766659019 1102 68-69 0.5685640820642135 1102 70-71 0.5531361040388418 1102 72-73 0.4655456773678601 1102 74-75 0.4002851755042194 1102 76-77 0.5687639527122776 1102 78-79 0.42322128002489023 1102 80-81 0.40856411827090255 1102 82-83 0.3387235156717274 1102 84-85 0.35256882343650986 1102 86-87 0.3558614748770985 1102 88-89 0.2866500592376333 1102 90-91 0.22415444597587708 1102 92-93 0.3939019648187063 1102 94-95 0.39258334018368046 1102 96-97 0.2658053857038283 1102 98-99 0.15917229775635988 1102 100-101 0.31731585531688467 1103 1 0.2993967414364995 1103 2 0.27339392848206145 1103 3 0.4663501148234097 1103 4 0.4110769411660513 1103 5 0.21113134747665185 1103 6 0.31755591884451917 1103 7 0.2154576316180652 1103 8 0.11945199746097757 1103 9 0.38223551647817544 1103 10-11 0.427692987007255 1103 12-13 0.4541652981245008 1103 14-15 0.5577973236386882 1103 16-17 0.5150097284336113 1103 18-19 0.62655131687238 1103 20-21 0.6330759239395718 1103 22-23 0.508988931085895 1103 24-25 0.5592539992687833 1103 26-27 0.629002942534548 1103 28-29 0.6053925737996408 1103 30-31 0.6249283750403691 1103 32-33 0.5915296800627985 1103 34-35 0.6273244859496288 1103 36-37 0.6100540227432063 1103 38-39 0.7414581799311364 1103 40-41 0.7411282958184486 1103 42-43 0.6066461508600156 1103 44-45 0.7840469953540747 1103 46-47 0.8505385242862005 1103 48-49 0.7729817987436363 1103 50-51 0.6694499774866856 1103 52-53 0.7796264243960351 1103 54-55 0.7644247652864777 1103 56-57 0.6721653909307079 1103 58-59 0.6606316090313129 1103 60-61 0.7763493055836932 1103 62-63 0.6426082486673366 1103 64-65 0.7356979836579924 1103 66-67 0.7729255321694026 1103 68-69 0.6438759710005755 1103 70-71 0.6246243782076562 1103 72-73 0.6566317680710192 1103 74-75 0.6411106717724415 1103 76-77 0.5869051890831756 1103 78-79 0.4928491223453264 1103 80-81 0.5482144923561094 1103 82-83 0.4875807530530665 1103 84-85 0.4538927239838486 1103 86-87 0.4416821728539908 1103 88-89 0.6650363336826075 1103 90-91 0.6633491498550725 1103 92-93 0.5926827169392617 1103 94-95 0.4297390349591943 1103 96-97 0.2905217181972155 1103 98-99 0.3461469410394322 1103 100-101 0.5230619631473807 1104 1 0.1788230139778868 1104 2 0.286886924586927 1104 3 0.19164467493817838 1104 4 0.22794700116297406 1104 5 0.23780995226250923 1104 6 0.21811257726029964 1104 7 0.22713480831102117 1104 8 0.212285481261695 1104 9 0.310724438192878 1104 10-11 0.2973873453454061 1104 12-13 0.3636202374681332 1104 14-15 0.42054898069444135 1104 16-17 0.34478846479688485 1104 18-19 0.4029685537125829 1104 20-21 0.4307310081630149 1104 22-23 0.4198586680246592 1104 24-25 0.38372450581773165 1104 26-27 0.3808074166205486 1104 28-29 0.4698326280756788 1104 30-31 0.5196597758676091 1104 32-33 0.5177428357623555 1104 34-35 0.5189386875747957 1104 36-37 0.530948312385398 1104 38-39 0.5964661202411037 1104 40-41 0.5959631844541491 1104 42-43 0.5810861952088899 1104 44-45 0.6774969061656364 1104 46-47 0.6316603132398697 1104 48-49 0.5589736752066514 1104 50-51 0.5247230438815151 1104 52-53 0.5049643803824111 1104 54-55 0.6591367372430952 1104 56-57 0.7325427336341477 1104 58-59 0.6213283864765771 1104 60-61 0.6396195503959774 1104 62-63 0.7760021589501989 1104 64-65 0.7633113723905538 1104 66-67 0.6124419617612986 1104 68-69 0.6328555468612294 1104 70-71 0.5998450164332922 1104 72-73 0.6538421651545896 1104 74-75 0.46837273121570533 1104 76-77 0.4861852147009209 1104 78-79 0.5109437826393233 1104 80-81 0.6473144605608319 1104 82-83 0.7200798078456039 1104 84-85 0.6472712159688001 1104 86-87 0.5884548814456103 1104 88-89 0.6189628942534604 1104 90-91 0.6033479047055508 1104 92-93 0.5593329896099881 1104 94-95 0.6023022574969126 1104 96-97 0.45422427665329934 1104 98-99 0.5893118076283308 1104 100-101 0.7378291953501801 1105 1 0.19545637904080237 1105 2 0.22513648170992795 1105 3 0.27595338568366756 1105 4 0.17683888713597185 1105 5 0.1408112163863322 1105 6 0.29182693297997275 1105 7 0.22636560089280522 1105 8 0.20883759980745253 1105 9 0.2807036657980433 1105 10-11 0.32485412143936543 1105 12-13 0.269688709396128 1105 14-15 0.426581350286952 1105 16-17 0.40558159949871 1105 18-19 0.4358798167739266 1105 20-21 0.46329832329846 1105 22-23 0.32245757541404174 1105 24-25 0.37312744040637114 1105 26-27 0.2767561220319976 1105 28-29 0.21957798735354572 1105 30-31 0.3094231646301324 1105 32-33 0.30047656857038163 1105 34-35 0.4035829169471299 1105 36-37 0.3314295142354027 1105 38-39 0.3903040315963153 1105 40-41 0.44016209206035484 1105 42-43 0.3670412212394325 1105 44-45 0.477733495720031 1105 46-47 0.497929754835404 1105 48-49 0.49012893367986266 1105 50-51 0.39068661846807373 1105 52-53 0.36821973773047745 1105 54-55 0.4925788022099553 1105 56-57 0.37586236506508186 1105 58-59 0.382214514506515 1105 60-61 0.4776887855685459 1105 62-63 0.46948682575708034 1105 64-65 0.5081735655477999 1105 66-67 0.48453019456824364 1105 68-69 0.48375534065510095 1105 70-71 0.36877218078457474 1105 72-73 0.3138064097484552 1105 74-75 0.26482209904538223 1105 76-77 0.22006655185336754 1105 78-79 0.29687441519994096 1105 80-81 0.38350152269805093 1105 82-83 0.3800630251382948 1105 84-85 0.4140967473669903 1105 86-87 0.3802135205285033 1105 88-89 0.3581652603116261 1105 90-91 0.5939416471842307 1105 92-93 0.5690968825042226 1105 94-95 0.49986350678949876 1105 96-97 0.5014035513107622 1105 98-99 0.6129581597746316 1105 100-101 0.7081102243860009 1106 1 0.23392328757894987 1106 2 0.21812441333084465 1106 3 0.23811582589815927 1106 4 0.22209005975074092 1106 5 0.31132431145945816 1106 6 0.2789029755258383 1106 7 0.2794817957841147 1106 8 0.27667026757429625 1106 9 0.4145854091203347 1106 10-11 0.3014188850477879 1106 12-13 0.3796092718312991 1106 14-15 0.4013399512116038 1106 16-17 0.401114220654911 1106 18-19 0.43368496956027514 1106 20-21 0.4507013752462399 1106 22-23 0.5199995984842118 1106 24-25 0.4669927860180465 1106 26-27 0.40329852512594044 1106 28-29 0.29037289839439495 1106 30-31 0.3394825722129724 1106 32-33 0.5345851188951869 1106 34-35 0.3611932078227795 1106 36-37 0.3234946809629591 1106 38-39 0.4661791360503713 1106 40-41 0.43417199367409154 1106 42-43 0.33336791360494544 1106 44-45 0.4270365405292367 1106 46-47 0.21230541357825672 1106 48-49 0.2273322631919612 1106 50-51 0.16728644504286194 1106 52-53 0.2589883620444411 1106 54-55 0.31168538942855406 1106 56-57 0.20806507520142503 1106 58-59 0.3208548482555713 1106 60-61 0.5519088970141866 1106 62-63 0.46073578121661996 1106 64-65 0.347581767762442 1106 66-67 0.33553830734081913 1106 68-69 0.3707680808994098 1106 70-71 0.3435628002862998 1106 72-73 0.2807010381859847 1106 74-75 0.38549827890370736 1106 76-77 0.2880981547138326 1106 78-79 0.4976471651862937 1106 80-81 0.5303024994221168 1106 82-83 0.5149856346628248 1106 84-85 0.4807050982318053 1106 86-87 0.5361224701931988 1106 88-89 0.5127929419986792 1106 90-91 0.5606914046828777 1106 92-93 0.5802254376160683 1106 94-95 0.5992334231842698 1106 96-97 0.631775061285861 1106 98-99 0.6167384514481178 1106 100-101 0.8908641264532413 1107 1 0.2436878057744316 1107 2 0.20872793169879245 1107 3 0.21468005828784342 1107 4 0.1960718765886469 1107 5 0.19823083325215407 1107 6 0.14497154427937886 1107 7 0.12969091446743164 1107 8 0.18850318205430483 1107 9 0.23818348239502996 1107 10-11 0.23336753855753045 1107 12-13 0.2962451404591846 1107 14-15 0.36663056150554496 1107 16-17 0.3645122914657577 1107 18-19 0.340630235285758 1107 20-21 0.3985302675023803 1107 22-23 0.34249125221570154 1107 24-25 0.41118135009028123 1107 26-27 0.2865293009901322 1107 28-29 0.30729719065546135 1107 30-31 0.46906378048163333 1107 32-33 0.3990783679650889 1107 34-35 0.4575972288147341 1107 36-37 0.41508265864053584 1107 38-39 0.6096887573576808 1107 40-41 0.44671757477149043 1107 42-43 0.5043994323178325 1107 44-45 0.19791530096976828 1107 46-47 0.43725120903000203 1107 48-49 0.4934966138461121 1107 50-51 0.44692955736947226 1107 52-53 0.48745360337787247 1107 54-55 0.6087678912148462 1107 56-57 0.5173466801488189 1107 58-59 0.47967571454280744 1107 60-61 0.5256104516991797 1107 62-63 0.4653986042880405 1107 64-65 0.5416717781105831 1107 66-67 0.3634936871422312 1107 68-69 0.5537769568941897 1107 70-71 0.3928296529731199 1107 72-73 0.4559888458583963 1107 74-75 0.5615637370553266 1107 76-77 0.5535926480244981 1107 78-79 0.5521019475057543 1107 80-81 0.5983784843433995 1107 82-83 0.6099814153315144 1107 84-85 0.6441156422483623 1107 86-87 0.7917438822216774 1107 88-89 0.8016816255673085 1107 90-91 0.7247995242778202 1107 92-93 0.8414844306433942 1107 94-95 0.8132528444275273 1107 96-97 0.8374536036762805 1107 98-99 0.8788559398064599 1107 100-101 1.0140547416748653 1108 1 0.3363657817291532 1108 2 0.31032786270596446 1108 3 0.2819605801632541 1108 4 0.24485981732295414 1108 5 0.21859063939081125 1108 6 0.30994160479716726 1108 7 0.3568330460904292 1108 8 0.3577504255351158 1108 9 0.38382490279560955 1108 10-11 0.34967768902152585 1108 12-13 0.4363916224635531 1108 14-15 0.5567228500659311 1108 16-17 0.5664225305131438 1108 18-19 0.5196333633867027 1108 20-21 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-2.1187530083253385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12266.0 3 3805.0 4 4007.0 5 4297.0 6 5171.0 7 6170.0 8 7779.0 9 9587.0 10 11836.0 11 14924.0 12 18701.0 13 25482.0 14 22387.0 15 22790.0 16 27020.0 17 33963.0 18 30838.0 19 24741.0 20 25948.0 21 27719.0 22 30420.0 23 34118.0 24 38857.0 25 44885.0 26 51166.0 27 59964.0 28 69437.0 29 82087.0 30 98333.0 31 119141.0 32 147495.0 33 187153.0 34 249200.0 35 351083.0 36 532182.0 37 800383.0 38 706326.0 39 100874.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31526700165242 18.87357522263581 14.05597501852713 23.75518275718463 2 15.0487690443149 20.290002191171684 40.59122021027821 24.070008554235216 3 19.097336744487333 24.408025126061155 29.985123248242672 26.50951488120884 4 12.93729047671639 16.59969185312557 37.18721631246945 33.275801357688586 5 13.879301890399804 37.08635431102887 34.4560853280289 14.578258470542435 6 32.00644529727201 35.79439402696183 17.72075576308335 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26.90158599763531 28.777556147203143 22.15941382571221 30-31 22.371323335996788 26.750857369915277 28.588541044515154 22.289278249572785 32-33 22.157274649388313 26.845592725783824 28.613572242481467 22.383560382346396 34-35 22.68913527788129 26.805208045982575 28.366398292691308 22.139258383444822 36-37 22.57469280447082 26.689595611923995 28.379592144735298 22.356119438869886 38-39 22.487360897790744 26.788856461707606 28.548412875764566 22.175369764737084 40-41 22.643934623090676 26.6202570402251 28.514148047892967 22.22166028879126 42-43 22.398639376637014 26.79363033763506 28.24086503944744 22.566865246280486 44-45 22.511974242791524 26.983050215368547 28.089914039046036 22.415061502793886 46-47 22.470408828164487 27.026918513056515 27.746725275140534 22.75594738363847 48-49 22.680243441036467 27.017718003855112 27.772428979787513 22.52960957532091 50-51 22.54183918357523 27.15256594762382 27.755538028975877 22.550056839825075 52-53 22.69490540976669 27.202418574027927 27.58522519601559 22.51745082018979 54-55 22.408625932268894 26.96119715278302 27.79486125094117 22.835315664006917 56-57 22.508831792339627 27.339388768507927 27.599411648831158 22.552367790321288 58-59 22.63024157628054 27.064766987138906 27.684976507911735 22.620014928668823 60-61 22.69378218938428 26.87122134745003 27.960876836809405 22.474119626356277 62-63 22.575559001625308 26.89739617333786 27.935128316931905 22.59191650810492 64-65 22.403018101497388 26.874073285738692 27.89564404822855 22.82726456453537 66-67 22.321784167072746 27.136439501881355 27.66408437543248 22.87769195561342 68-69 22.232991489915747 27.022654385722088 27.860344236035488 22.884009888326673 70-71 22.300765070485866 26.942869166032878 27.76863643756998 22.98772932591127 72-73 22.37448662364818 26.787518297901336 27.787333158514006 23.050661919936477 74-75 22.250944959402087 27.050265660450744 27.6341454407504 23.064643939396763 76-77 22.41467826043719 26.812487957685637 27.70728008403205 23.065553697845125 78-79 22.358765231834088 26.87972695485613 27.704557041126044 23.056950772183736 80-81 22.1943204927652 27.108723195924085 27.672291453146762 23.024664858163955 82-83 22.334084982467942 27.000760847420697 27.63050783115974 23.03464633895162 84-85 22.366506617846948 26.798529292762552 27.49608226990802 23.33888181948248 86-87 22.301152042800105 26.623761317813653 27.883015697018216 23.192070942368026 88-89 22.387915166593718 26.554066459970382 27.739634284069115 23.318384089366784 90-91 22.29261177425435 26.60937669398258 27.908888230221496 23.18912330154158 92-93 22.383429494444506 26.554815314838688 27.801890413510392 23.259864777206417 94-95 22.331164531541923 26.477084583269868 27.715968826453373 23.475782058734843 96-97 22.35022803690815 26.27476803099228 27.82923112253392 23.54577280956565 98-99 22.21848238797107 26.277186208392045 27.682351216524424 23.82198018711246 100-101 22.554756802388958 25.64617147727291 28.295685551946253 23.503386168391874 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2934.0 1 1696.5 2 524.0 3 584.5 4 512.0 5 555.0 6 939.5 7 1769.5 8 2773.5 9 3420.5 10 3487.0 11 3501.0 12 3895.0 13 4387.0 14 4893.5 15 5286.5 16 5662.0 17 5928.5 18 6083.5 19 6346.0 20 6849.0 21 7554.0 22 8709.0 23 10699.0 24 13094.5 25 16261.0 26 20870.5 27 26646.0 28 32087.0 29 37519.0 30 43680.5 31 50714.5 32 58239.0 33 66427.0 34 75906.5 35 84007.5 36 92462.5 37 101505.5 38 107865.5 39 111912.5 40 116428.5 41 124364.5 42 132095.0 43 138040.0 44 143758.0 45 147562.5 46 148447.0 47 151934.5 48 167294.5 49 170647.0 50 160852.5 51 156511.0 52 148613.0 53 143588.0 54 139131.5 55 128285.5 56 116097.5 57 102854.0 58 90056.0 59 78958.0 60 67410.0 61 54675.0 62 43484.5 63 34589.0 64 26684.0 65 20058.0 66 14675.0 67 11419.0 68 8420.5 69 5523.0 70 3926.0 71 2799.0 72 1972.5 73 1368.0 74 995.0 75 776.0 76 532.0 77 304.5 78 184.5 79 124.5 80 78.5 81 58.0 82 45.0 83 33.0 84 24.0 85 25.5 86 22.5 87 17.0 88 14.5 89 14.5 90 10.0 91 7.0 92 9.5 93 10.0 94 6.5 95 4.5 96 4.5 97 4.0 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3297172209159801 2 0.08954732836040574 3 0.09991206056565712 4 0.10441416381471617 5 0.11574363243048023 6 0.059368394493086674 7 0.06154523562449985 8 0.06241102471085737 9 0.06493418147681354 10-11 0.06280681400747794 12-13 0.060914446433010805 14-15 0.05920760509133457 16-17 0.06034549931911873 18-19 0.06869417979470904 20-21 0.07067312627781193 22-23 0.08650469814263505 24-25 0.0956449572114665 26-27 0.0904873279398796 28-29 0.08785285543424888 30-31 0.08194075281597901 32-33 0.07351786184727233 34-35 0.3287401160899481 36-37 0.5087005618971171 38-39 0.4829618892012601 40-41 0.4637908451462009 42-43 0.4944150419722181 44-45 0.504136616570461 46-47 0.498595566417773 48-49 0.5165792425829705 50-51 0.5127202969409198 52-53 0.5115824027131356 54-55 0.5024421436443042 56-57 0.4810695216267929 58-59 0.46390216088587544 60-61 0.42207217959928806 62-63 0.49317820042027877 64-65 0.5183355575867242 66-67 0.5289847633489216 68-69 0.5406110739371511 70-71 0.5380013382625592 72-73 0.5259173963001122 74-75 0.5281437110936029 76-77 0.5016629334665823 78-79 0.48197241595970863 80-81 0.4874639924503192 82-83 0.4963816200398015 84-85 0.49811319821251654 86-87 0.49807609296595845 88-89 0.5043716164653295 90-91 0.5186571363902284 92-93 0.5260410804553062 94-95 0.5322747618770802 96-97 0.5235921341824662 98-99 0.5264863434140042 100-101 0.5042231954790968 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4042555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.76248720933499 #Duplication Level Percentage of deduplicated Percentage of total 1 69.70240511953396 33.291602329816264 2 14.956758650186613 14.28743987445297 3 6.037594915554726 8.651116497879856 4 3.0034151572731633 5.738023121343293 5 1.722311728117141 4.113094594234129 6 1.1225369676116421 3.2169094534534044 7 0.7247267837835935 2.4230327618508456 8 0.505701219327248 1.9322838415890233 9 0.39503852799364775 1.6981220376442012 >10 1.6761076724847053 13.57989044958223 >50 0.08805130671722997 2.9246315883096683 >100 0.0599385677811741 5.339483269569737 >500 0.0036956754445439644 1.181827726171085 >1k 0.0015615529005642732 1.0193844729957484 >5k 1.0410352670428239E-4 0.3425129840368308 >10k+ 5.2051763352141196E-5 0.26064499707063465 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10484 0.2593409366106336 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8558 0.21169780002993155 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5219 0.12910152119142473 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.947366207757223E-5 0.0 5 0.0 0.0 0.0 4.947366207757223E-5 0.0 6 0.0 0.0 0.0 4.947366207757223E-5 0.0 7 0.0 0.0 0.0 4.947366207757223E-5 0.0 8 0.0 0.0 0.0 4.947366207757223E-5 0.0 9 0.0 0.0 0.0 7.421049311635834E-5 0.0 10-11 2.4736831038786115E-5 0.0 0.0 7.421049311635834E-5 0.0 12-13 4.947366207757223E-5 0.0 0.0 9.894732415514446E-5 0.0 14-15 7.421049311635834E-5 0.0 0.0 1.360525707133236E-4 0.0 16-17 7.421049311635834E-5 0.0 0.0 1.7315781727150282E-4 2.4736831038786115E-5 18-19 9.894732415514446E-5 0.0 0.0 2.597367259072542E-4 2.4736831038786115E-5 20-21 9.894732415514446E-5 0.0 0.0 3.9578929662057783E-4 2.4736831038786115E-5 22-23 9.894732415514446E-5 0.0 0.0 4.947366207757223E-4 2.4736831038786115E-5 24-25 1.2368415519393056E-4 0.0 0.0 6.307891914890458E-4 2.4736831038786115E-5 26-27 1.2368415519393056E-4 0.0 0.0 0.0011378942277841613 2.4736831038786115E-5 28-29 1.2368415519393056E-4 0.0 0.0 0.0035868405006239867 3.710524655817917E-5 30-31 1.484209862327167E-4 0.0 0.0 0.011317100200244647 4.947366207757223E-5 32-33 1.484209862327167E-4 0.0 0.0 0.02544183072339152 4.947366207757223E-5 34-35 1.484209862327167E-4 0.0 0.0 0.0475689260875857 4.947366207757223E-5 36-37 1.484209862327167E-4 0.0 0.0 0.08233654211259958 4.947366207757223E-5 38-39 1.484209862327167E-4 2.4736831038786115E-5 0.0 0.13393757165950743 4.947366207757223E-5 40-41 1.484209862327167E-4 2.4736831038786115E-5 0.0 0.19154965114884026 4.947366207757223E-5 42-43 1.6078940175210974E-4 2.4736831038786115E-5 0.0 0.2531196236043789 4.947366207757223E-5 44-45 1.731578172715028E-4 2.4736831038786115E-5 0.0 0.3280598532363814 4.947366207757223E-5 46-47 2.2263147934907502E-4 2.4736831038786115E-5 0.0 0.4193882334315798 4.947366207757223E-5 48-49 2.2263147934907502E-4 2.4736831038786115E-5 0.0 0.5194116097369115 4.947366207757223E-5 50-51 2.2263147934907502E-4 2.4736831038786115E-5 0.0 0.6238134051361082 4.947366207757223E-5 52-53 2.2263147934907502E-4 2.4736831038786115E-5 0.0 0.7351415132261652 6.184207759696528E-5 54-55 2.2263147934907502E-4 2.4736831038786115E-5 0.0 0.8585040896165915 7.421049311635834E-5 56-57 2.473683103878611E-4 2.4736831038786115E-5 0.0 1.0050698135213991 8.657890863575141E-5 58-59 2.473683103878611E-4 2.4736831038786115E-5 0.0 1.1735771065576102 1.1131573967453751E-4 60-61 2.473683103878611E-4 2.4736831038786115E-5 0.0 1.3615770224523847 1.2368415519393056E-4 62-63 2.473683103878611E-4 2.4736831038786115E-5 0.0 1.5519516741268826 1.2368415519393056E-4 64-65 2.473683103878611E-4 2.4736831038786115E-5 0.0 1.7545463203345408 1.2368415519393056E-4 66-67 2.473683103878611E-4 2.4736831038786115E-5 0.0 1.9871591110077662 1.2368415519393056E-4 68-69 2.721051414266473E-4 2.4736831038786115E-5 0.0 2.2565308326046276 1.2368415519393056E-4 70-71 2.844735569460403E-4 2.4736831038786115E-5 0.0 2.551900963623253 1.2368415519393056E-4 72-73 2.968419724654334E-4 2.4736831038786115E-5 0.0 2.8536779338809244 1.2368415519393056E-4 74-75 3.092103879848264E-4 2.4736831038786115E-5 0.0 3.1742920009746314 1.484209862327167E-4 76-77 3.215788035042195E-4 2.4736831038786115E-5 0.0 3.519197636148426 1.484209862327167E-4 78-79 3.215788035042195E-4 2.4736831038786115E-5 0.0 3.9105590400130605 1.484209862327167E-4 80-81 3.7105246558179173E-4 2.4736831038786115E-5 0.0 4.343255688543508 1.484209862327167E-4 82-83 3.7105246558179173E-4 3.710524655817917E-5 0.0 4.794863644403106 1.484209862327167E-4 84-85 3.9578929662057783E-4 4.947366207757223E-5 0.0 5.25290317633279 1.9789464831028892E-4 86-87 3.9578929662057783E-4 7.421049311635834E-5 0.0 5.742655325654196 1.9789464831028892E-4 88-89 3.9578929662057783E-4 7.421049311635834E-5 0.0 6.2754990346451685 1.9789464831028892E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12685 0.0 27.24005 1 GTATCAA 17815 0.0 26.856022 1 GTACATG 13355 0.0 23.66995 1 TACATGG 13575 0.0 22.643621 2 ATCAACG 21255 0.0 22.350172 3 TATCAAC 21445 0.0 22.240494 2 CAACGCA 21615 0.0 21.977928 5 TCAACGC 21690 0.0 21.945602 4 ACATGGG 13725 0.0 21.63696 3 AACGCAG 22130 0.0 21.433651 6 GAGTACT 10150 0.0 20.218254 12-13 ACGCAGA 24225 0.0 19.150385 7 AGTACTT 10915 0.0 18.887959 12-13 CATGGGG 7405 0.0 18.740604 4 CGCAGAG 24375 0.0 18.663538 8 GTACTTT 11425 0.0 17.942122 14-15 GCAGAGT 25065 0.0 17.469852 9 GTGTAGC 2145 0.0 17.25974 1 AGAGTAC 20055 0.0 17.101372 10-11 CATGGGA 7435 0.0 15.925756 4 >>END_MODULE