FastQCFastQC Report
Fri 27 May 2016
SRR938993_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938993_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4042555
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106950.2645604079598175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90540.22396726822516946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55060.13620099169955635No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA181150.028.9310151
GGTATCA126950.027.6219541
ATCAACG214100.024.182163
TATCAAC215600.024.0799482
TCAACGC217550.023.7986684
CAACGCA217900.023.782225
GTACATG134050.023.5288351
TACATGG133700.023.213112
AACGCAG223700.023.144396
ACATGGG134900.021.9864483
GAGTACT112550.021.12558612-13
ACGCAGA248100.020.5621597
CGCAGAG248150.020.1946648
AGTACTT119250.019.5207812-13
CATGGGG73450.019.3827954
GTACTTT125700.018.76487514-15
GCAGAGT257300.018.7392279
AGAGTAC204400.017.9476310-11
AGTACAT101350.015.6858492
GTATTAG11300.015.60028651
Produced by FastQC (version 0.11.5)