##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938993_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4042555 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.203733035172064 33.0 31.0 34.0 30.0 34.0 2 32.45238865024718 34.0 31.0 34.0 31.0 34.0 3 32.544145719724284 34.0 31.0 34.0 31.0 34.0 4 36.08508480404101 37.0 35.0 37.0 35.0 37.0 5 36.00856042774928 37.0 35.0 37.0 35.0 37.0 6 36.041690465559526 37.0 35.0 37.0 35.0 37.0 7 35.97798422037548 37.0 35.0 37.0 35.0 37.0 8 35.94979536456523 37.0 35.0 37.0 35.0 37.0 9 37.63846033016248 39.0 37.0 39.0 35.0 39.0 10-11 37.549771122470815 39.0 37.0 39.0 35.0 39.0 12-13 37.461382962012884 39.0 37.0 39.0 34.0 39.0 14-15 38.80651592866393 40.0 38.0 41.0 34.5 41.0 16-17 38.888572697217484 40.0 38.0 41.0 35.0 41.0 18-19 38.9141414278841 40.0 38.0 41.0 35.0 41.0 20-21 38.88619857985853 40.0 38.0 41.0 35.0 41.0 22-23 38.81601153231063 40.0 38.0 41.0 34.0 41.0 24-25 38.744794814170746 40.0 38.0 41.0 34.0 41.0 26-27 38.59882833999785 40.0 38.0 41.0 34.0 41.0 28-29 38.44350701474686 40.0 38.0 41.0 34.0 41.0 30-31 38.2624774431022 40.0 38.0 41.0 34.0 41.0 32-33 38.184627296350946 40.0 38.0 41.0 33.0 41.0 34-35 38.097964529857975 40.0 38.0 41.0 33.0 41.0 36-37 37.97666154201984 40.0 37.5 41.0 33.0 41.0 38-39 37.84808184427918 40.0 37.0 41.0 33.0 41.0 40-41 37.68105356637077 40.0 37.0 41.0 33.0 41.0 42-43 37.511843499964755 40.0 37.0 41.0 32.0 41.0 44-45 37.28316423647916 40.0 37.0 41.0 31.5 41.0 46-47 37.00057327605933 40.0 36.0 41.0 31.0 41.0 48-49 36.702158783244755 39.5 36.0 41.0 30.5 41.0 50-51 36.45305320521304 39.0 36.0 41.0 30.0 41.0 52-53 36.193457108190245 39.0 35.0 40.5 29.5 41.0 54-55 35.89279317164516 39.0 35.0 40.0 29.0 41.0 56-57 35.76767353814605 38.5 35.0 40.0 28.5 41.0 58-59 35.494180165761506 38.0 35.0 40.0 28.0 41.0 60-61 35.14336675691487 38.0 34.0 40.0 28.0 41.0 62-63 34.72424728915253 37.5 34.0 40.0 26.5 41.0 64-65 34.320030896301965 37.0 34.0 39.5 26.0 41.0 66-67 33.87592920813694 36.0 33.0 39.0 26.0 40.5 68-69 33.395806117665686 36.0 32.5 39.0 25.5 40.0 70-71 32.879129783020886 35.0 32.0 38.0 24.0 40.0 72-73 32.36383660828362 35.0 31.5 37.5 22.5 39.5 74-75 31.83181774892364 35.0 31.0 37.0 21.5 39.0 76-77 29.916120498051356 33.0 28.5 35.0 19.5 37.5 78-79 30.874035108984295 34.0 30.5 36.0 20.0 38.0 80-81 30.853954491651937 34.0 31.0 35.5 19.5 37.0 82-83 30.441440623566038 34.0 30.0 35.0 18.0 37.0 84-85 29.982974628669247 34.0 30.0 35.0 13.5 36.0 86-87 29.277344773293127 34.0 29.0 35.0 7.0 36.0 88-89 28.59083586988922 33.5 29.0 35.0 2.0 35.5 90-91 28.01214664982913 33.0 27.0 35.0 2.0 35.0 92-93 27.601416430945278 33.0 27.0 35.0 2.0 35.0 94-95 27.05346606292308 32.5 26.0 34.0 2.0 35.0 96-97 26.20514056085817 32.0 25.0 34.0 2.0 35.0 98-99 24.976993015555756 32.0 19.0 34.0 2.0 35.0 100-101 19.36335522955161 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14117693067986892 1101 2 0.1763968922157133 1101 3 0.1984945459687637 1101 4 0.12405150370758378 1101 5 0.09769409490739633 1101 6 0.14132530607940907 1101 7 0.1577558998005273 1101 8 0.10615321749637019 1101 9 0.17675764975451358 1101 10-11 0.2706340426042573 1101 12-13 0.2892043522950374 1101 14-15 0.2643238523100351 1101 16-17 0.20030026232812048 1101 18-19 0.2765001341810276 1101 20-21 0.3380474970357028 1101 22-23 0.2640680467824268 1101 24-25 0.19970993806315107 1101 26-27 0.17134446359654731 1101 28-29 0.21462855312509532 1101 30-31 0.14788100485996836 1101 32-33 0.22102519716360547 1101 34-35 0.34202527059638044 1101 36-37 0.4073250384963245 1101 38-39 0.36512919193474147 1101 40-41 0.3186021524528613 1101 42-43 0.3105092725155032 1101 44-45 0.3784937492518239 1101 46-47 0.37521930283323 1101 48-49 0.4464170830751115 1101 50-51 0.39585747519188885 1101 52-53 0.33245525956392896 1101 54-55 0.4002595676442269 1101 56-57 0.44064120497572645 1101 58-59 0.305468704903312 1101 60-61 0.3782179975129125 1101 62-63 0.48922724450330435 1101 64-65 0.39978826436001214 1101 66-67 0.32066591487137686 1101 68-69 0.45337701918706586 1101 70-71 0.47674707587525234 1101 72-73 0.4551649042253061 1101 74-75 0.40927220078827276 1101 76-77 0.28179822237701657 1101 78-79 0.5892826199672534 1101 80-81 0.7233945127602688 1101 82-83 0.6205506313935594 1101 84-85 0.7552449543492905 1101 86-87 0.9053749077488824 1101 88-89 0.9186240374972492 1101 90-91 0.8625155524352515 1101 92-93 0.7340715461940412 1101 94-95 0.63146289477422 1101 96-97 0.8105165527681919 1101 98-99 0.9647260303994045 1101 100-101 1.1220157264622728 1102 1 0.1149242148853844 1102 2 0.17886655611082602 1102 3 0.15717868058558793 1102 4 0.08021868420892986 1102 5 0.17222441086749996 1102 6 0.13138735913503297 1102 7 0.1509898265189733 1102 8 0.1524743455238493 1102 9 0.19952096163008548 1102 10-11 0.2513270600674886 1102 12-13 0.22047623205843792 1102 14-15 0.2927204152396712 1102 16-17 0.2980289316170328 1102 18-19 0.23074280987598428 1102 20-21 0.27345291352325063 1102 22-23 0.25233132129729086 1102 24-25 0.23689083801461663 1102 26-27 0.3223202422886047 1102 28-29 0.3002149582868441 1102 30-31 0.22363900922981372 1102 32-33 0.23492037831872636 1102 34-35 0.2955662008535924 1102 36-37 0.3047127967298593 1102 38-39 0.29100570045348206 1102 40-41 0.28835880640831846 1102 42-43 0.34986673700533544 1102 44-45 0.3317676183476408 1102 46-47 0.3465126032091348 1102 48-49 0.3155219320657139 1102 50-51 0.2581520681195286 1102 52-53 0.3328356529528449 1102 54-55 0.2695841161404857 1102 56-57 0.2775429497562598 1102 58-59 0.2528220812893167 1102 60-61 0.23711901708149696 1102 62-63 0.5127867036689082 1102 64-65 0.42636543964228935 1102 66-67 0.3135809540615426 1102 68-69 0.25379793072249157 1102 70-71 0.4831713308721177 1102 72-73 0.5433522357896194 1102 74-75 0.698722281245935 1102 76-77 0.4492149962324703 1102 78-79 0.5005313775274338 1102 80-81 0.5490547220973596 1102 82-83 0.7141031884729259 1102 84-85 0.7583233191020504 1102 86-87 0.8606991865051548 1102 88-89 0.6349230048417063 1102 90-91 0.7942163659270527 1102 92-93 0.9226386925207031 1102 94-95 0.9918894882796288 1102 96-97 0.9768735470703653 1102 98-99 1.3290543885443853 1102 100-101 1.4934277952979755 1103 1 0.10741074541930118 1103 2 0.1092735043737747 1103 3 0.10192648018923478 1103 4 0.13718037718354026 1103 5 0.142986923214373 1103 6 0.1384049007200474 1103 7 0.1641147361660984 1103 8 0.1362759795576025 1103 9 0.0958811102917636 1103 10-11 0.10357663863683797 1103 12-13 0.13712912295062551 1103 14-15 0.21475752706805906 1103 16-17 0.16796973792445868 1103 18-19 0.18280149402696821 1103 20-21 0.20243031455430582 1103 22-23 0.1857413883955843 1103 24-25 0.20130622980087765 1103 26-27 0.19854993274532973 1103 28-29 0.14016390472641405 1103 30-31 0.19962475503079702 1103 32-33 0.24598108270054553 1103 34-35 0.23796271885642994 1103 36-37 0.22638093389291924 1103 38-39 0.2578382814674569 1103 40-41 0.346374373141515 1103 42-43 0.39991763714144923 1103 44-45 0.26511750421939695 1103 46-47 0.33206629966540646 1103 48-49 0.36931422450368956 1103 50-51 0.3615506543252991 1103 52-53 0.46252749265944715 1103 54-55 0.6047134863293664 1103 56-57 0.5923522422562542 1103 58-59 0.5429825696021666 1103 60-61 0.5660138499019425 1103 62-63 0.6353131958337883 1103 64-65 0.5567025258207181 1103 66-67 0.5694006515390555 1103 68-69 0.680509513977114 1103 70-71 0.6815135271605968 1103 72-73 0.6426413193905276 1103 74-75 0.6809632010657438 1103 76-77 0.39600409549994353 1103 78-79 0.7291047540010922 1103 80-81 0.6921239732937998 1103 82-83 0.7432804948995262 1103 84-85 0.8595034113684044 1103 86-87 0.8956250425858023 1103 88-89 0.6616639980242667 1103 90-91 0.7963826739913991 1103 92-93 1.0183495322076368 1103 94-95 1.2333587183856274 1103 96-97 0.9993879165537045 1103 98-99 1.169394242843154 1103 100-101 1.3572385440323842 1104 1 0.01673634804756574 1104 2 0.02802182562007971 1104 3 0.030398171190206824 1104 4 0.05447798578737917 1104 5 -5.044334908959058E-4 1104 6 0.10127242900748712 1104 7 0.07746913493331675 1104 8 0.055109178023371896 1104 9 0.1061655773209722 1104 10-11 0.08437063276166157 1104 12-13 0.0815408195845535 1104 14-15 0.1638086820080602 1104 16-17 0.20716798076038145 1104 18-19 0.13003643955948263 1104 20-21 0.11383090638322813 1104 22-23 0.11313935066159786 1104 24-25 0.24872242400563493 1104 26-27 0.11546151663507942 1104 28-29 0.149336494362295 1104 30-31 0.08902087453224539 1104 32-33 0.1822953720534457 1104 34-35 0.26296359811917824 1104 36-37 0.29550133928410105 1104 38-39 0.19385486237764837 1104 40-41 0.25854864528922405 1104 42-43 0.10329728209514855 1104 44-45 0.2177304887747269 1104 46-47 0.38141368106491313 1104 48-49 0.26374203150041353 1104 50-51 0.33172989062035896 1104 52-53 0.27347185347938563 1104 54-55 0.40364729514530495 1104 56-57 0.3189207761082926 1104 58-59 0.29496030808729756 1104 60-61 0.2229051710396206 1104 62-63 0.1371574494687664 1104 64-65 0.22120205796784376 1104 66-67 0.2848240335750347 1104 68-69 0.294378437456011 1104 70-71 0.22911238111249332 1104 72-73 0.28092077518927994 1104 74-75 0.3493913359423857 1104 76-77 0.3670980841506477 1104 78-79 0.46122998030488915 1104 80-81 0.43790712837787993 1104 82-83 0.5550262966325796 1104 84-85 0.6002840931714388 1104 86-87 0.6015602931090385 1104 88-89 0.5672599311220132 1104 90-91 0.6362682521251024 1104 92-93 0.5754213432988848 1104 94-95 0.6872913416150119 1104 96-97 0.5592416015581065 1104 98-99 0.9199980978147195 1104 100-101 1.2741133974295025 1105 1 0.012658301953663909 1105 2 0.054459197283321714 1105 3 0.08567621338114861 1105 4 0.05051214965512685 1105 5 0.061090975332817266 1105 6 0.011431279283627305 1105 7 0.0331640886290927 1105 8 0.011226409787354896 1105 9 0.10652578756015885 1105 10-11 0.10641324977984823 1105 12-13 0.09910452448644946 1105 14-15 0.08784036966756048 1105 16-17 0.07051494913994105 1105 18-19 0.12326427023193531 1105 20-21 0.0703883047600371 1105 22-23 0.0628289031010496 1105 24-25 -0.006311168512837639 1105 26-27 -0.03721192635683934 1105 28-29 0.0039197021144232735 1105 30-31 0.029813992971760683 1105 32-33 0.022121208098973 1105 34-35 0.12673691014527577 1105 36-37 0.12707893003323534 1105 38-39 0.12164585479073509 1105 40-41 0.12170683531544313 1105 42-43 0.10894884251831627 1105 44-45 0.11963160740504719 1105 46-47 0.19091965367050534 1105 48-49 0.1950191218039521 1105 50-51 0.15190461833503122 1105 52-53 0.0886007919406211 1105 54-55 0.10556248067661755 1105 56-57 0.027057651662566684 1105 58-59 0.032931074952948336 1105 60-61 0.08468052235978973 1105 62-63 -0.024013594289868934 1105 64-65 -0.21199913503546242 1105 66-67 -0.04394790157520134 1105 68-69 -0.03571210026279914 1105 70-71 0.11764791101455074 1105 72-73 -0.07840059305671332 1105 74-75 0.05532941122171309 1105 76-77 -0.2438505995777156 1105 78-79 -0.004214501241722957 1105 80-81 0.10868770857385357 1105 82-83 0.08400275747618124 1105 84-85 0.12597339842373145 1105 86-87 0.1137434761424565 1105 88-89 0.32464871316148347 1105 90-91 0.14971420378482847 1105 92-93 0.37265780709138596 1105 94-95 0.415622351244469 1105 96-97 0.39223089661934907 1105 98-99 0.530276096639497 1105 100-101 0.6982079660336993 1106 1 0.004792754782421582 1106 2 0.013962520916599885 1106 3 0.049297860878667166 1106 4 -0.032916714514726664 1106 5 -0.012601616933174853 1106 6 0.03364902240435441 1106 7 0.03740261338102613 1106 8 0.06143363821733061 1106 9 0.049204136283400146 1106 10-11 0.12103104245063889 1106 12-13 0.07756704490485333 1106 14-15 0.0318060226575696 1106 16-17 -0.03590089134245744 1106 18-19 -0.012468251824138576 1106 20-21 0.0740594183346559 1106 22-23 0.00862906308896072 1106 24-25 0.15788665590358164 1106 26-27 0.0961071298404832 1106 28-29 0.1308098479511699 1106 30-31 0.17803690945471118 1106 32-33 0.19601200060599666 1106 34-35 0.008746616498179094 1106 36-37 0.056361703408896346 1106 38-39 0.08100063322218176 1106 40-41 0.26579883046121466 1106 42-43 0.18860528843526225 1106 44-45 0.20004993798793436 1106 46-47 0.1531075512782465 1106 48-49 0.18599854383189296 1106 50-51 0.11389987958561676 1106 52-53 0.17985688596816374 1106 54-55 0.1405726951276023 1106 56-57 0.18198636046358985 1106 58-59 0.1678505351092383 1106 60-61 0.20644375915448876 1106 62-63 0.20787438898069155 1106 64-65 0.20647238207821061 1106 66-67 0.2456590782646444 1106 68-69 0.07844252531931062 1106 70-71 0.19340806352796136 1106 72-73 0.17054436858398248 1106 74-75 0.2528685473710013 1106 76-77 0.015275219782918015 1106 78-79 0.22315413860912514 1106 80-81 0.2302336389602182 1106 82-83 0.1486949802163018 1106 84-85 0.21955766292027334 1106 86-87 0.44035579521125356 1106 88-89 0.5365130131828977 1106 90-91 0.6499199625096317 1106 92-93 0.6358009080853257 1106 94-95 0.7963518394091444 1106 96-97 0.8134557489139809 1106 98-99 0.8184201949333776 1106 100-101 1.000136324205048 1107 1 0.040837239469638575 1107 2 0.06943748827067964 1107 3 0.11862886588026811 1107 4 0.08865084406314594 1107 5 0.06508951569550447 1107 6 0.06666144017358988 1107 7 0.09634082019601209 1107 8 0.08332950388860638 1107 9 -0.010525580128252443 1107 10-11 0.055087051763962336 1107 12-13 0.07126807602318763 1107 14-15 0.10255296749212306 1107 16-17 0.09263010380377779 1107 18-19 0.14675298896725053 1107 20-21 0.155099067729779 1107 22-23 0.13401018881886984 1107 24-25 0.08332999367183902 1107 26-27 0.12857442232330385 1107 28-29 0.17087963630704195 1107 30-31 0.14105000478080143 1107 32-33 0.10066897205005176 1107 34-35 0.1057958564025796 1107 36-37 0.17858449026182655 1107 38-39 0.12214219332763321 1107 40-41 0.07396169664989571 1107 42-43 0.19313260013605316 1107 44-45 0.18690841484012566 1107 46-47 0.20815981555005436 1107 48-49 0.1374546751054524 1107 50-51 0.19872691779982432 1107 52-53 0.07993264914355791 1107 54-55 0.015208508319020098 1107 56-57 0.09362293744636219 1107 58-59 0.16393687433115645 1107 60-61 0.22464232027011377 1107 62-63 0.3008613675698939 1107 64-65 0.24020547395491576 1107 66-67 0.19392453272050147 1107 68-69 0.22185590275543632 1107 70-71 0.3315172487350466 1107 72-73 0.24910939556306033 1107 74-75 0.18246346076691822 1107 76-77 0.21494737466009894 1107 78-79 0.4936679422901058 1107 80-81 0.47174800932511474 1107 82-83 0.23157250257567696 1107 84-85 0.43415839714552007 1107 86-87 0.5417406290250355 1107 88-89 0.871579506148624 1107 90-91 0.689856549347784 1107 92-93 0.9132042330415935 1107 94-95 1.0081237737723647 1107 96-97 1.054021539684424 1107 98-99 1.1009860411402919 1107 100-101 1.0139184465594937 1108 1 0.1410298976953328 1108 2 0.1324756962457414 1108 3 0.13417008696822563 1108 4 0.05963100010619371 1108 5 0.14247008782495385 1108 6 0.14064720294897626 1108 7 0.09738635972422571 1108 8 0.15195208541300786 1108 9 0.1706503430767583 1108 10-11 0.13327373806377807 1108 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-0.6774441451704547 2316 54-55 -0.5755178649652208 2316 56-57 -0.6369634244613351 2316 58-59 -0.6679547798694685 2316 60-61 -0.6362384105376933 2316 62-63 -0.6546598638708048 2316 64-65 -0.5775296506524299 2316 66-67 -0.6463779493067676 2316 68-69 -0.5464287018283187 2316 70-71 -0.5059288575759879 2316 72-73 -0.5005644420348361 2316 74-75 -0.7119825155790274 2316 76-77 -0.7116199748027796 2316 78-79 -0.7787677923166818 2316 80-81 -0.6854170000315598 2316 82-83 -0.7657626008944938 2316 84-85 -0.9966936944151961 2316 86-87 -0.9652346659773663 2316 88-89 -1.1458670582026649 2316 90-91 -1.3583500700581084 2316 92-93 -1.3603016223245987 2316 94-95 -1.1989935596132 2316 96-97 -0.983894221103089 2316 98-99 -1.022149663637773 2316 100-101 -0.5768208569255258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 28.0 5 72.0 6 160.0 7 441.0 8 1038.0 9 2057.0 10 3358.0 11 5163.0 12 7632.0 13 10363.0 14 12853.0 15 17026.0 16 21719.0 17 30409.0 18 30454.0 19 22867.0 20 22018.0 21 24179.0 22 25990.0 23 29695.0 24 33790.0 25 40263.0 26 48305.0 27 58458.0 28 71593.0 29 86393.0 30 104929.0 31 127090.0 32 158108.0 33 201461.0 34 270595.0 35 386529.0 36 608428.0 37 925449.0 38 621255.0 39 32367.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.01976911772631 19.684997520105522 14.655692047026847 23.63954131514131 2 15.420668364437837 20.805258060805603 39.95240633708138 23.821667237675186 3 18.989599399389743 24.588558473539628 30.311424334362798 26.11041779270783 4 13.21223829978813 17.199073358309285 36.811224584452155 32.77746375745043 5 13.808346454160796 37.33487856071222 34.45427458624558 14.4025003988814 6 31.98212516589137 36.230675896802886 17.42180378498252 14.36539515232322 7 28.127755837582914 32.839850045330245 21.092774247969416 17.939619869117426 8 25.620331696167398 36.01051810055769 19.874930582267897 18.49421962100701 9 26.142254094254746 17.075413939946397 20.586732895408968 36.19559907038989 10-11 24.82149284301636 26.699649108051716 27.495210331090114 20.983647717841812 12-13 25.582323555276304 24.00431162965006 27.988413268341432 22.424951546732203 14-15 22.565247952299078 25.37843026932572 26.256458994714066 25.799862783661137 16-17 22.257982962325105 28.59905854192986 26.87656479865072 22.266393697094315 18-19 22.029540404266534 27.418021036820743 28.973247205772818 21.5791913531399 20-21 22.734075355808393 26.6681962273859 28.817097108140764 21.78063130866494 22-23 22.45514284901504 26.566663904387198 28.608244043680298 22.36994920291746 24-25 22.4848258019128 26.702309159583066 28.71525741282798 22.09760762567615 26-27 22.37877341898216 26.924303299349866 28.585814809152826 22.11110847251515 28-29 22.05556881724553 27.003528708947684 28.844926043059406 22.095976430747385 30-31 22.33388290326291 26.852448513378295 28.65058113000318 22.16308745335561 32-33 22.124077470807446 26.885274288166766 28.649876130318574 22.340772110707213 34-35 22.562826738749276 26.84954759107369 28.485829765199604 22.101795904977426 36-37 22.54281741299195 26.73889914340431 28.46840839473671 22.249875048867025 38-39 22.428597422848554 26.86412763073438 28.66749783559208 22.039777110824986 40-41 22.566138528183508 26.690598521489317 28.5971811669117 22.14608178341548 42-43 22.368013849414336 26.841700729058466 28.30164827920087 22.488637142326333 44-45 22.46504316033331 27.041225879626023 28.10263983663514 22.39109112340553 46-47 22.445706972008466 27.119191848228873 27.794428607191378 22.640672572571287 48-49 22.647395108986316 27.131809696831166 27.7454127884339 22.475382405748615 50-51 22.53182596159388 27.253098490476148 27.708963662139553 22.50611188579042 52-53 22.710043524454214 27.31653620049696 27.497782961518148 22.475637313530676 54-55 22.412533657550732 27.09164130110784 27.68771235023395 22.80811269110748 56-57 22.482390468404265 27.482297705287866 27.52510479140049 22.510207034907378 58-59 22.608355357483877 27.183507761967007 27.598519937504513 22.609616943044607 60-61 22.658538190266608 27.058307540687732 27.8535711599962 22.42958310904946 62-63 22.528962531300188 27.041100109276233 27.824153696684544 22.60578366273903 64-65 22.37647392336526 27.013251859542308 27.746184502911177 22.864089714181254 66-67 22.28363442781494 27.303787446537637 27.532238212676408 22.880339912971017 68-69 22.24371906813523 27.139149528869037 27.67091816887937 22.94621323411636 70-71 22.24217110218661 27.0941026157962 27.596334496376674 23.067391785640517 72-73 22.35351157869219 26.976182142234308 27.531771367365444 23.138534911708067 74-75 22.219499796539566 27.194756781293016 27.35720355072473 23.22853987144269 76-77 22.291443931034856 26.989281949017453 27.534023770462174 23.185250349485514 78-79 22.224947494192822 27.076372757835053 27.50314787465893 23.195531873313197 80-81 22.055763613356085 27.332165505084483 27.4031356225587 23.208935259000732 82-83 22.204573865361617 27.197543038690014 27.35516614556464 23.242716950383723 84-85 22.232997868673056 27.02082743771756 27.21753486411554 23.528639829493844 86-87 22.11692236992263 26.872970742542645 27.559993473197398 23.450113414337327 88-89 22.17500733919218 26.84474617576219 27.407109058967098 23.573137426078524 90-91 22.030308761238445 26.977055031689233 27.515794945294648 23.47684126177768 92-93 22.086230119318103 26.86292456132322 27.393109060977526 23.65773625838115 94-95 21.982650662613086 26.87598754983745 27.25628274954901 23.885079038000452 96-97 21.955495967278118 26.686860166404664 27.351563553247882 24.006080313069333 98-99 21.734101329480985 26.706130157783875 27.24508386404143 24.31468464869371 100-101 21.903536366625033 26.13618569913611 27.984430082183497 23.975847852055352 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 735.0 1 513.5 2 349.5 3 415.0 4 385.5 5 414.5 6 677.5 7 1347.0 8 2242.0 9 2769.0 10 2711.0 11 2653.5 12 2939.0 13 3307.0 14 3754.0 15 4063.5 16 4306.5 17 4569.5 18 4873.0 19 5408.5 20 6204.0 21 7213.5 22 8644.0 23 10780.0 24 13264.0 25 16591.5 26 21423.0 27 26975.5 28 32150.0 29 37951.5 30 44216.5 31 51307.5 32 58751.0 33 66118.0 34 75780.0 35 84552.0 36 92950.0 37 102197.5 38 108664.5 39 112252.5 40 116722.5 41 124820.0 42 133042.5 43 139442.5 44 144512.0 45 148511.0 46 150107.0 47 153596.0 48 168807.0 49 172287.0 50 162761.5 51 157860.5 52 149645.0 53 144696.0 54 140014.0 55 129302.5 56 116746.0 57 103322.5 58 90699.5 59 79206.5 60 67074.0 61 53738.5 62 42502.0 63 33834.5 64 25871.5 65 19246.5 66 13919.0 67 10687.0 68 7736.5 69 4787.0 70 3328.5 71 2389.0 72 1693.5 73 1202.5 74 877.0 75 674.0 76 417.0 77 193.0 78 110.0 79 57.0 80 24.0 81 14.5 82 7.5 83 2.5 84 3.0 85 3.5 86 2.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40009350522132664 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.286838397993349E-4 16-17 0.0025231567659561834 18-19 5.936839449308667E-4 20-21 0.0 22-23 0.0 24-25 4.947366207757223E-5 26-27 2.4736831038786115E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013011573126401495 36-37 0.024118410262816463 38-39 0.011292363369205862 40-41 0.006035786773463812 42-43 0.005392629166455372 44-45 0.001731578172715028 46-47 0.0011626310588229473 48-49 0.00299315655569312 50-51 5.442102828532945E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.689471138920806E-4 60-61 0.0014594730312883807 62-63 0.0011749994743423405 64-65 6.926312690860113E-4 66-67 0.0026839461677082934 68-69 0.0025107883504367906 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09303522153687457 78-79 0.0035868405006239867 80-81 2.1026306382968197E-4 82-83 1.2368415519393057E-5 84-85 7.421049311635834E-5 86-87 0.0945936418923181 88-89 0.4435190121099156 90-91 0.396519033136222 92-93 0.0 94-95 9.276311639544793E-4 96-97 0.0 98-99 0.0 100-101 3.9578929662057783E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4042555.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.901205990046535 #Duplication Level Percentage of deduplicated Percentage of total 1 67.7186742596574 31.083688165653967 2 15.892761118909043 14.58993803739293 3 6.333774786316276 8.721837034837993 4 3.2922394377025195 6.044710423941533 5 1.858008517208447 4.264241583982292 6 1.1771482290631055 3.2419514005826446 7 0.8013745830164565 2.574884186715821 8 0.5481697097453357 2.012932061161974 9 0.3955723703251137 1.6341523968837615 >10 1.8154182211501015 14.139438528478157 >50 0.09495469030238435 3.0307751270409433 >100 0.06574097319935922 5.644859393548247 >500 0.004433109545484024 1.367940802357904 >1k 0.0015678069348023294 1.021941683253992 >5k 1.0812461619326313E-4 0.3613100604190672 >10k+ 5.4062308096631566E-5 0.26539911374875846 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10695 0.2645604079598175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9054 0.22396726822516946 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5506 0.13620099169955635 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 2.4736831038786115E-5 0.0 0.0 0.0 8 0.0 2.4736831038786115E-5 0.0 0.0 0.0 9 0.0 2.4736831038786115E-5 0.0 2.4736831038786115E-5 0.0 10-11 0.0 2.4736831038786115E-5 0.0 2.4736831038786115E-5 0.0 12-13 1.2368415519393057E-5 2.4736831038786115E-5 0.0 4.947366207757223E-5 0.0 14-15 2.4736831038786115E-5 2.4736831038786115E-5 0.0 8.657890863575141E-5 0.0 16-17 2.4736831038786115E-5 2.4736831038786115E-5 0.0 1.6078940175210974E-4 0.0 18-19 4.947366207757223E-5 2.4736831038786115E-5 0.0 2.2263147934907502E-4 0.0 20-21 8.657890863575141E-5 2.4736831038786115E-5 0.0 2.968419724654334E-4 0.0 22-23 1.2368415519393056E-4 2.4736831038786115E-5 0.0 4.3289454317875704E-4 0.0 24-25 1.2368415519393056E-4 2.4736831038786115E-5 0.0 5.318418673339014E-4 0.0 26-27 1.2368415519393056E-4 2.4736831038786115E-5 0.0 0.0010265784881096237 0.0 28-29 1.360525707133236E-4 2.4736831038786115E-5 0.0 0.00343841951439127 0.0 30-31 1.484209862327167E-4 2.4736831038786115E-5 0.0 0.011304731784725255 0.0 32-33 1.6078940175210974E-4 2.4736831038786115E-5 0.0 0.025553146463066057 0.0 34-35 1.8552623279089587E-4 2.4736831038786115E-5 0.0 0.048076031123880814 0.0 36-37 1.9789464831028892E-4 2.4736831038786115E-5 0.0 0.08359812049557767 0.0 38-39 1.9789464831028892E-4 2.4736831038786115E-5 0.0 0.13618862328403697 2.4736831038786115E-5 40-41 1.9789464831028892E-4 2.4736831038786115E-5 0.0 0.19459228136661097 2.4736831038786115E-5 42-43 1.9789464831028892E-4 2.4736831038786115E-5 0.0 0.25683014826019684 2.4736831038786115E-5 44-45 1.9789464831028892E-4 2.4736831038786115E-5 0.0 0.3327474827182314 2.4736831038786115E-5 46-47 1.9789464831028892E-4 2.4736831038786115E-5 0.0 0.42518902031017514 2.4736831038786115E-5 48-49 1.9789464831028892E-4 2.4736831038786115E-5 0.0 0.526226606688097 2.4736831038786115E-5 50-51 1.9789464831028892E-4 2.4736831038786115E-5 0.0 0.631358138602938 2.4736831038786115E-5 52-53 1.9789464831028892E-4 2.4736831038786115E-5 0.0 0.7437746672587016 2.4736831038786115E-5 54-55 1.9789464831028892E-4 2.4736831038786115E-5 0.0 0.8687698744976877 2.4736831038786115E-5 56-57 2.2263147934907502E-4 2.4736831038786115E-5 0.0 1.0173887553787146 2.4736831038786115E-5 58-59 2.2263147934907502E-4 2.4736831038786115E-5 0.0 1.1879739422221838 2.4736831038786115E-5 60-61 2.2263147934907502E-4 2.4736831038786115E-5 0.0 1.378039383508697 2.4736831038786115E-5 62-63 2.2263147934907502E-4 2.4736831038786115E-5 0.0 1.5704300869128558 2.4736831038786115E-5 64-65 2.2263147934907502E-4 2.4736831038786115E-5 0.0 1.7758694686899745 2.4736831038786115E-5 66-67 2.2263147934907502E-4 2.4736831038786115E-5 0.0 2.0109806792981173 2.4736831038786115E-5 68-69 2.2263147934907502E-4 2.4736831038786115E-5 0.0 2.2820221369900966 2.4736831038786115E-5 70-71 2.597367259072542E-4 2.4736831038786115E-5 0.0 2.579717530126368 4.947366207757223E-5 72-73 2.721051414266473E-4 2.4736831038786115E-5 0.0 2.884722656834601 4.947366207757223E-5 74-75 2.844735569460403E-4 2.4736831038786115E-5 0.0 3.2090101433375677 4.947366207757223E-5 76-77 2.968419724654334E-4 2.4736831038786115E-5 0.0 3.557304724363676 4.947366207757223E-5 78-79 2.968419724654334E-4 3.710524655817917E-5 0.0 3.950842969359724 4.947366207757223E-5 80-81 3.7105246558179173E-4 4.947366207757223E-5 0.0 4.385840143176778 4.947366207757223E-5 82-83 3.7105246558179173E-4 4.947366207757223E-5 0.0 4.839179677209092 7.421049311635834E-5 84-85 3.9578929662057783E-4 4.947366207757223E-5 0.0 5.299062103051164 7.421049311635834E-5 86-87 4.2052612765936393E-4 4.947366207757223E-5 0.0 5.792351619211118 8.657890863575141E-5 88-89 4.2052612765936393E-4 4.947366207757223E-5 0.0 6.3282379584198605 9.894732415514446E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18115 0.0 28.931015 1 GGTATCA 12695 0.0 27.621954 1 ATCAACG 21410 0.0 24.18216 3 TATCAAC 21560 0.0 24.079948 2 TCAACGC 21755 0.0 23.798668 4 CAACGCA 21790 0.0 23.78222 5 GTACATG 13405 0.0 23.528835 1 TACATGG 13370 0.0 23.21311 2 AACGCAG 22370 0.0 23.14439 6 ACATGGG 13490 0.0 21.986448 3 GAGTACT 11255 0.0 21.125586 12-13 ACGCAGA 24810 0.0 20.562159 7 CGCAGAG 24815 0.0 20.194664 8 AGTACTT 11925 0.0 19.52078 12-13 CATGGGG 7345 0.0 19.382795 4 GTACTTT 12570 0.0 18.764875 14-15 GCAGAGT 25730 0.0 18.739227 9 AGAGTAC 20440 0.0 17.94763 10-11 AGTACAT 10135 0.0 15.685849 2 GTATTAG 1130 0.0 15.6002865 1 >>END_MODULE