FastQCFastQC Report
Fri 27 May 2016
SRR938992_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938992_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4055612
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109210.2692811837029775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83410.20566563073587907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49820.12284212592328851No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA186450.029.372411
GGTATCA129500.026.8748631
TATCAAC216750.025.0452232
ATCAACG216700.024.963643
CAACGCA217250.024.83575
TCAACGC218950.024.6422584
AACGCAG222150.024.2854986
GTACATG132500.024.2624021
TACATGG133050.023.4418092
ACATGGG134250.022.3509273
GAGTACT100300.021.96473112-13
ACGCAGA244150.021.6709947
CGCAGAG245350.021.314518
GCAGAGT251950.019.704519
AGTACTT106200.019.40755512-13
GTACTTT113850.019.26802814-15
AGAGTAC197650.017.92253710-11
CATGGGG71850.017.719284
CATGGGA74300.016.6891044
AGTACAT100750.016.4885562
Produced by FastQC (version 0.11.5)