##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938992_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4055612 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.69538136291144 33.0 31.0 34.0 30.0 34.0 2 31.77101926910168 33.0 31.0 34.0 30.0 34.0 3 31.81868852345836 34.0 31.0 34.0 30.0 34.0 4 35.35605575681303 37.0 35.0 37.0 33.0 37.0 5 35.3877836439975 37.0 35.0 37.0 33.0 37.0 6 35.45695002381885 37.0 35.0 37.0 33.0 37.0 7 35.35950061297777 37.0 35.0 37.0 33.0 37.0 8 35.338908652997375 37.0 35.0 37.0 33.0 37.0 9 36.937253119874384 39.0 37.0 39.0 33.0 39.0 10-11 36.81942737618885 39.0 37.0 39.0 32.5 39.0 12-13 36.68672409983992 39.0 37.0 39.0 32.0 39.0 14-15 37.89096109292507 40.0 38.0 41.0 32.0 41.0 16-17 37.808350379671424 40.0 38.0 41.0 32.0 41.0 18-19 37.73151647642821 40.0 38.0 41.0 32.0 41.0 20-21 37.60406234620077 40.0 37.0 41.0 32.0 41.0 22-23 37.6928653431344 40.0 38.0 41.0 32.0 41.0 24-25 37.627449445361144 40.0 37.0 41.0 32.0 41.0 26-27 37.550333956995885 40.0 37.0 41.0 32.0 41.0 28-29 37.41679677444489 40.0 37.0 41.0 31.0 41.0 30-31 37.23998486541612 40.0 37.0 41.0 31.0 41.0 32-33 37.071205652809 40.0 36.5 41.0 30.5 41.0 34-35 36.75856418217522 40.0 36.0 41.0 30.0 41.0 36-37 36.49932784497136 39.0 36.0 41.0 30.0 41.0 38-39 36.30010008353857 39.0 35.5 41.0 30.0 41.0 40-41 36.14647123541404 39.0 35.0 41.0 29.0 41.0 42-43 35.95702485839375 39.0 35.0 41.0 28.5 41.0 44-45 35.91403837447961 39.0 35.0 41.0 28.0 41.0 46-47 35.71680266751356 39.0 35.0 41.0 27.0 41.0 48-49 35.56487837100788 39.0 35.0 41.0 26.5 41.0 50-51 34.411345932500446 38.0 34.0 40.0 24.5 40.5 52-53 34.56079082515783 38.0 34.0 39.5 25.0 40.5 54-55 35.05640073557333 39.0 35.0 40.0 26.0 41.0 56-57 34.963012240815935 39.0 34.5 41.0 25.0 41.0 58-59 34.76618473364809 38.0 34.0 40.0 25.0 41.0 60-61 34.50506520347607 38.0 34.0 40.0 24.0 41.0 62-63 34.14837723628395 37.5 34.0 40.0 23.0 41.0 64-65 33.74210624685004 37.0 33.5 40.0 22.0 41.0 66-67 33.3690731509819 36.5 33.0 39.5 22.0 41.0 68-69 32.982607803705086 36.0 32.5 39.0 20.5 41.0 70-71 32.57030159689832 35.5 32.0 39.0 20.0 40.0 72-73 32.12737682993344 35.0 32.0 38.0 18.5 40.0 74-75 31.65856151919858 35.0 31.0 37.0 17.5 39.5 76-77 31.239073905491946 35.0 31.0 37.0 13.5 39.0 78-79 30.804325709658613 35.0 31.0 36.0 9.5 39.0 80-81 30.369649636109173 34.5 30.5 36.0 7.0 37.5 82-83 29.982788047771827 34.0 30.0 35.0 4.5 37.0 84-85 29.623661854240495 34.0 30.0 35.0 2.0 37.0 86-87 29.22281593998637 34.0 29.0 35.0 2.0 36.0 88-89 28.806257477293194 34.0 29.0 35.0 2.0 36.0 90-91 28.378738769882325 34.0 29.0 35.0 2.0 35.5 92-93 27.95418225412096 34.0 28.0 35.0 2.0 35.0 94-95 27.476968580820845 34.0 27.0 35.0 2.0 35.0 96-97 26.878024697628867 33.0 25.5 35.0 2.0 35.0 98-99 26.14007479019196 33.0 24.5 35.0 2.0 35.0 100-101 23.457094391672577 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40499855203715995 1101 2 0.39593232788099186 1101 3 0.3597505727972994 1101 4 0.3232096736556258 1101 5 0.1482550728843819 1101 6 0.2850517080738513 1101 7 0.3109698442818498 1101 8 0.3520781108001927 1101 9 0.3532105293876313 1101 10-11 0.47592759547527663 1101 12-13 0.5602723891612555 1101 14-15 0.4060211338539901 1101 16-17 0.5746300040320875 1101 18-19 0.6255760402257451 1101 20-21 0.6324585468708364 1101 22-23 0.5904608402834484 1101 24-25 0.6369167987239237 1101 26-27 0.5577444713676059 1101 28-29 0.5425638177879861 1101 30-31 0.5909090256270559 1101 32-33 0.6282619274828889 1101 34-35 0.5911585851981016 1101 36-37 0.49621505629051654 1101 38-39 0.5426477103351459 1101 40-41 0.6079416571174647 1101 42-43 0.43386610427835137 1101 44-45 0.6238014623818984 1101 46-47 0.5776520607557387 1101 48-49 0.5261673696893183 1101 50-51 0.5349723301434253 1101 52-53 0.5623920137720617 1101 54-55 0.5336954748360725 1101 56-57 0.5572446357589627 1101 58-59 0.4524399974041202 1101 60-61 0.5206498965373143 1101 62-63 0.5445855378938731 1101 64-65 0.5200378180561032 1101 66-67 0.4101725675651551 1101 68-69 0.35708112186408414 1101 70-71 0.35183899763311643 1101 72-73 0.18815230322587695 1101 74-75 0.32184384678712874 1101 76-77 0.3082774068118681 1101 78-79 0.21742909942869204 1101 80-81 0.13866808326127966 1101 82-83 0.06561010597324568 1101 84-85 -0.10572092286363954 1101 86-87 -0.02062920885855135 1101 88-89 -0.1351679738999252 1101 90-91 -0.09795913695311498 1101 92-93 0.052258264987397496 1101 94-95 0.10946156222068026 1101 96-97 0.010921313949005196 1101 98-99 -0.17365575941234823 1101 100-101 0.19673458320394133 1102 1 0.3702771246270622 1102 2 0.26207981301338634 1102 3 0.2597303896972001 1102 4 0.28490889104087813 1102 5 0.25112105942433516 1102 6 0.3342497238399176 1102 7 0.30985662871334796 1102 8 0.23170137053768514 1102 9 0.4884211645139658 1102 10-11 0.2841322512898401 1102 12-13 0.3381165209317416 1102 14-15 0.5396507594725222 1102 16-17 0.4637365176080266 1102 18-19 0.4891712383435234 1102 20-21 0.533731805124674 1102 22-23 0.5630021496607043 1102 24-25 0.5695361480541408 1102 26-27 0.5155232993229504 1102 28-29 0.6061474201951356 1102 30-31 0.5910806708601086 1102 32-33 0.5909224744038752 1102 34-35 0.48885365055319596 1102 36-37 0.5375380051727134 1102 38-39 0.5787218225146233 1102 40-41 0.5848793201906872 1102 42-43 0.5141678703406356 1102 44-45 0.6339171131863637 1102 46-47 0.6529742690439662 1102 48-49 0.7429927663193681 1102 50-51 0.569724387732542 1102 52-53 0.4959988787062386 1102 54-55 0.5823579752514192 1102 56-57 0.5976937015316679 1102 58-59 0.572648237496594 1102 60-61 0.5191744859390823 1102 62-63 0.45928452322817037 1102 64-65 0.3870438712084834 1102 66-67 0.45127713968001615 1102 68-69 0.3984133008225541 1102 70-71 0.3714032382118475 1102 72-73 0.3551945314401337 1102 74-75 0.334914386858717 1102 76-77 0.2181943580041832 1102 78-79 0.3095276892234544 1102 80-81 0.14213913890441532 1102 82-83 0.046091024397288294 1102 84-85 0.022284988213943535 1102 86-87 -0.011388808582278642 1102 88-89 0.13691207779944037 1102 90-91 0.10300122855243643 1102 92-93 0.02512659254817251 1102 94-95 -0.10769153630224437 1102 96-97 -0.17688431267813698 1102 98-99 -0.0948119351743344 1102 100-101 0.17946360635146874 1103 1 0.10031373340196481 1103 2 0.2366299086510537 1103 3 0.33237951062835336 1103 4 0.33207192313677325 1103 5 0.2951274205596306 1103 6 0.23756107833064277 1103 7 0.2931933055462892 1103 8 0.2546534600552164 1103 9 0.3299908781780161 1103 10-11 0.3406418413632082 1103 12-13 0.3951343908339666 1103 14-15 0.4820029199333504 1103 16-17 0.5277049970624716 1103 18-19 0.5015634019950923 1103 20-21 0.5620685583319727 1103 22-23 0.5473133632831733 1103 24-25 0.581975188588558 1103 26-27 0.4561436288189 1103 28-29 0.49561712757951426 1103 30-31 0.5041478819910878 1103 32-33 0.5298234304411054 1103 34-35 0.4465989098271592 1103 36-37 0.5909327095445533 1103 38-39 0.6608861020583419 1103 40-41 0.5661611534468065 1103 42-43 0.43893719432574585 1103 44-45 0.4985544283392116 1103 46-47 0.3802949378117759 1103 48-49 0.40045941194066614 1103 50-51 0.3160595896917968 1103 52-53 0.45894468855446036 1103 54-55 0.5616361866658224 1103 56-57 0.5291636023977588 1103 58-59 0.5399743033855984 1103 60-61 0.48211785180789235 1103 62-63 0.4451412483296977 1103 64-65 0.3282740414554084 1103 66-67 0.23317135949898926 1103 68-69 0.21005730127740208 1103 70-71 0.35776688537588797 1103 72-73 0.4131716632605702 1103 74-75 0.3099855932171387 1103 76-77 0.23546416145053328 1103 78-79 0.31508669011109447 1103 80-81 0.258704635006783 1103 82-83 0.17838339222297606 1103 84-85 0.06386926392197623 1103 86-87 0.23666658149550557 1103 88-89 0.22915101751970468 1103 90-91 0.21172026087236517 1103 92-93 0.24845967130032065 1103 94-95 0.167249562096778 1103 96-97 0.06853894551980133 1103 98-99 0.1858284892634252 1103 100-101 0.4419274091494856 1104 1 0.09660487710880616 1104 2 0.13804866295654605 1104 3 0.13908146133151789 1104 4 0.1460662440870948 1104 5 0.1858289008089713 1104 6 0.2160623468757663 1104 7 0.16528228852594395 1104 8 0.14489221536882724 1104 9 0.26965349558145135 1104 10-11 0.19557858212635182 1104 12-13 0.24855610227775315 1104 14-15 0.29087299066944183 1104 16-17 0.11868018062347119 1104 18-19 0.2716005342430279 1104 20-21 0.1862171209275374 1104 22-23 0.22534518612371102 1104 24-25 0.30620221616051424 1104 26-27 0.22752609097256737 1104 28-29 0.2775915214501765 1104 30-31 0.18813128169200866 1104 32-33 0.17287768384769464 1104 34-35 0.14519981377057434 1104 36-37 0.12228039171939287 1104 38-39 0.20007977781484954 1104 40-41 0.3325388270526659 1104 42-43 0.26516684299755866 1104 44-45 0.3241210731633757 1104 46-47 0.361328424042604 1104 48-49 0.3128604066144547 1104 50-51 0.34881224859596927 1104 52-53 0.3330421733067155 1104 54-55 0.36465650018968887 1104 56-57 0.3949794197272638 1104 58-59 0.3594648691063185 1104 60-61 0.36097716931381996 1104 62-63 0.26362823524100776 1104 64-65 0.2071446162491739 1104 66-67 0.3243187627619193 1104 68-69 0.3298252667326551 1104 70-71 0.32191443985612267 1104 72-73 0.2701318595070177 1104 74-75 0.10253108442133296 1104 76-77 0.10833947438633373 1104 78-79 0.15936193919958797 1104 80-81 0.0593513409899451 1104 82-83 0.11722792403741877 1104 84-85 0.23659352757773888 1104 86-87 0.17820815785183797 1104 88-89 0.12406608380329232 1104 90-91 0.18782759155099527 1104 92-93 0.03377092690795891 1104 94-95 0.11030986419534017 1104 96-97 -0.02048738309219189 1104 98-99 -0.005846494354702969 1104 100-101 0.28289906430683587 1105 1 0.09303558992433736 1105 2 0.14008628040869553 1105 3 0.11596710115781406 1105 4 0.02766169953505937 1105 5 0.08161428618701194 1105 6 0.17426671410692052 1105 7 0.1195497521099611 1105 8 0.21825987704634997 1105 9 0.2813749563228285 1105 10-11 0.1743679313403632 1105 12-13 0.2429311116975157 1105 14-15 0.2281271512555847 1105 16-17 0.3190383937060304 1105 18-19 0.23105047254768607 1105 20-21 0.21534377954289852 1105 22-23 0.19475661316363357 1105 24-25 0.36950563997380215 1105 26-27 0.2453333079193598 1105 28-29 0.31433819439012467 1105 30-31 0.318548759004301 1105 32-33 0.355757831051541 1105 34-35 0.3202812824278283 1105 36-37 0.3771534722994758 1105 38-39 0.35090321247970735 1105 40-41 0.2541373754591021 1105 42-43 0.24356970345991158 1105 44-45 0.24796722600139987 1105 46-47 0.4132429784436127 1105 48-49 0.3986986212959138 1105 50-51 0.4003611196333665 1105 52-53 0.480819404283217 1105 54-55 0.45898085586156867 1105 56-57 0.46059482999967827 1105 58-59 0.3131176727224627 1105 60-61 0.5175803080113823 1105 62-63 0.398058400235918 1105 64-65 0.5410293524335756 1105 66-67 0.5073893366040139 1105 68-69 0.4408674888922519 1105 70-71 0.24804250144269702 1105 72-73 0.45435429826545715 1105 74-75 0.36803650463447113 1105 76-77 0.3850201401849631 1105 78-79 0.3793236769442352 1105 80-81 0.3910446309082758 1105 82-83 0.23243469683012918 1105 84-85 0.3368665532447075 1105 86-87 0.47427312177496717 1105 88-89 0.47163820969456083 1105 90-91 0.5263406910820088 1105 92-93 0.5410687689796667 1105 94-95 0.559843332037417 1105 96-97 0.5919291414316241 1105 98-99 0.5998254803616732 1105 100-101 0.805951073845204 1106 1 0.09252107081435668 1106 2 0.14283512990975922 1106 3 0.13612872196167913 1106 4 0.20141426887194314 1106 5 0.15900865510216988 1106 6 0.25003327732082425 1106 7 0.21083403873322482 1106 8 0.20926056139745697 1106 9 0.30784038997907714 1106 10-11 0.23445008653705912 1106 12-13 0.162216625740065 1106 14-15 0.1895775763029235 1106 16-17 0.33734445207953456 1106 18-19 0.3061004159359513 1106 20-21 0.3513410612135601 1106 22-23 0.28785697675417055 1106 24-25 0.2263128797638032 1106 26-27 0.2064792625319356 1106 28-29 0.16564798951271342 1106 30-31 0.15015766064270508 1106 32-33 0.2940347719162517 1106 34-35 0.17646664207013885 1106 36-37 0.025420178099771817 1106 38-39 0.0985427797622691 1106 40-41 0.20922765251670938 1106 42-43 0.2288066547065597 1106 44-45 0.31809347202685956 1106 46-47 0.21661716704436884 1106 48-49 0.15965175205122506 1106 50-51 0.16488835242662248 1106 52-53 0.027214219365028214 1106 54-55 0.05396237431224904 1106 56-57 0.2887653807494104 1106 58-59 0.3462968749851214 1106 60-61 0.30709567132183224 1106 62-63 0.2485710150147895 1106 64-65 0.02070991694471047 1106 66-67 0.27722863585594837 1106 68-69 0.21118023219916893 1106 70-71 0.14701512911505432 1106 72-73 0.21359505702982062 1106 74-75 0.2044551934792942 1106 76-77 0.15336900626864747 1106 78-79 0.1278251527531573 1106 80-81 0.13169765561237767 1106 82-83 0.20503251666031375 1106 84-85 0.17848683191034098 1106 86-87 0.22896198872466655 1106 88-89 0.2638994022814458 1106 90-91 0.18227145532537037 1106 92-93 0.23101578279455381 1106 94-95 0.2055301301650303 1106 96-97 0.27383669877758265 1106 98-99 0.22135130801926195 1106 100-101 0.5491222664721427 1107 1 0.029487593566305037 1107 2 0.013638995694847722 1107 3 0.0820321462665774 1107 4 0.16767355662930328 1107 5 0.09387777899851812 1107 6 0.1724699606934763 1107 7 0.06962018895724498 1107 8 0.07162873024397953 1107 9 0.0640401004892297 1107 10-11 0.21551384160051867 1107 12-13 0.180010741777771 1107 14-15 0.12493183532199481 1107 16-17 0.23260231050120694 1107 18-19 0.24345279517041973 1107 20-21 0.17036308138577283 1107 22-23 0.09909843838229904 1107 24-25 0.2184012809619702 1107 26-27 0.22134618387016758 1107 28-29 0.28803977893960564 1107 30-31 0.22631730245720405 1107 32-33 0.1517805562061838 1107 34-35 0.04121510829848063 1107 36-37 -0.03555439159949714 1107 38-39 -0.021476133809272824 1107 40-41 0.10410673006373372 1107 42-43 0.16462554530910722 1107 44-45 0.2186097020876403 1107 46-47 0.173357340786076 1107 48-49 0.049374503948214965 1107 50-51 0.1766457485123567 1107 52-53 0.0679975199744618 1107 54-55 0.07563250834123636 1107 56-57 0.07918229065975169 1107 58-59 -0.030614624066366503 1107 60-61 -0.04537139502548371 1107 62-63 -0.10197878172972707 1107 64-65 -0.002901638095273995 1107 66-67 -0.1192766050625238 1107 68-69 -0.06553899271170138 1107 70-71 0.04550610007337497 1107 72-73 -0.017494390351998845 1107 74-75 0.026723156623742028 1107 76-77 0.12793625265716457 1107 78-79 0.1470042639989444 1107 80-81 0.06474331674271028 1107 82-83 0.11980795196114613 1107 84-85 0.1402649013499193 1107 86-87 0.18091049792238678 1107 88-89 0.266667851787453 1107 90-91 0.2176072611591202 1107 92-93 0.22696105067203476 1107 94-95 0.25879168979001577 1107 96-97 0.2158912439782661 1107 98-99 0.32274492603480454 1107 100-101 0.49226893960937446 1108 1 0.20978297712800753 1108 2 0.26269652766844587 1108 3 0.19294900276372928 1108 4 0.20329259425848534 1108 5 0.2065186960774028 1108 6 0.23111096595499703 1108 7 0.25770932386627265 1108 8 0.21265192394923105 1108 9 0.392769297388341 1108 10-11 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-3.824561787230767 2316 100-101 -4.41285074070122 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13523.0 3 3803.0 4 3762.0 5 4354.0 6 5040.0 7 6421.0 8 7592.0 9 9319.0 10 11241.0 11 14190.0 12 18036.0 13 25285.0 14 24009.0 15 21469.0 16 26108.0 17 32870.0 18 29162.0 19 23507.0 20 24094.0 21 25934.0 22 28756.0 23 32594.0 24 36532.0 25 41975.0 26 49097.0 27 57146.0 28 67197.0 29 79075.0 30 95267.0 31 114766.0 32 142024.0 33 181239.0 34 241717.0 35 340734.0 36 522753.0 37 813655.0 38 760427.0 39 120915.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.280757909063574 18.882493630018832 14.062277446310159 23.774471014607435 2 15.051457393929358 20.24791257043139 40.60586975347687 24.094760282162383 3 19.066432499829705 24.436424987141557 29.965901675596008 26.531240837432733 4 12.902130957758509 16.604759542163738 37.17720297292427 33.31590652715348 5 13.898652256240895 37.11003887546743 34.45328118670765 14.538027681584028 6 32.04282876981424 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22.20509005580203 28-29 22.155435310586093 26.91497588894286 28.769907129380062 22.15968167109099 30-31 22.378821304447698 26.78412091560103 28.598516524594803 22.23854125535647 32-33 22.189533630089684 26.842605786563762 28.560554773517055 22.407305809829495 34-35 22.629780971018747 26.794910891843955 28.4004988215119 22.174809315625403 36-37 22.577739513750867 26.67371839280173 28.40258187927137 22.345960214176028 38-39 22.47603589237743 26.76097519239795 28.60483355477258 22.158155360452046 40-41 22.604130384392747 26.6514038373579 28.51776194200703 22.22670383624232 42-43 22.386757853860782 26.820119373228557 28.25205257976606 22.541070193144595 44-45 22.512996743078997 26.96733815317462 28.11783250747698 22.401832596269404 46-47 22.463145249529052 27.016409428229338 27.76895461566008 22.751490706581528 48-49 22.659492875239927 27.035863665385566 27.76348316752798 22.541160291846523 50-51 22.55058426680639 27.171379762572823 27.74475182999991 22.533284140620882 52-53 22.6787356871371 27.22289874516466 27.589957398277555 22.50840816942069 54-55 22.417520795658465 26.988392823815982 27.781739800395904 22.812346580129645 56-57 22.516612695011116 27.33363409188707 27.5950188135261 22.554734399575715 58-59 22.62549495520997 27.077175512626756 27.692437953402898 22.604891578760373 60-61 22.695464552318327 26.904811702002174 27.924249122518347 22.475474623161155 62-63 22.563567925462472 26.918922632050062 27.92854745335856 22.58896198912891 64-65 22.40259462388038 26.89773981299666 27.870426082975523 22.82923948014744 66-67 22.317077361245207 27.161640459381818 27.653950457135252 22.867331722237722 68-69 22.23682610885471 27.001793817655827 27.880528766831 22.88085130665846 70-71 22.297422268556 26.951192690322284 27.76263618594142 22.98874885518029 72-73 22.40890681228544 26.791672599877646 27.773319255704937 23.026101332131972 74-75 22.25355086036679 27.063068757526636 27.579342167232003 23.104038214874574 76-77 22.401125564425417 26.831131852009854 27.699004527804888 23.068738055759834 78-79 22.37533919725404 26.901544207665218 27.66713872625247 23.055977868828272 80-81 22.166441910250594 27.134536087011668 27.67445980662228 23.02456219611546 82-83 22.3455896722175 27.005848759568963 27.622009529033992 23.026552039179546 84-85 22.394625726339083 26.764670539648378 27.523707824217542 23.316995909795 86-87 22.3202871209955 26.64441224341301 27.88397560007212 23.151325035519367 88-89 22.383400296690787 26.564118768581928 27.774816602084375 23.277664332642907 90-91 22.319050173487835 26.651142167001034 27.906257655015022 23.12355000449611 92-93 22.396861650419503 26.55717687464253 27.836243367965313 23.20971810697266 94-95 22.33608242588967 26.509089073302896 27.693507251920845 23.461321248886588 96-97 22.337460013867233 26.30696202139126 27.81355391910615 23.542024045635358 98-99 22.209920107647775 26.29797783337022 27.695349751074446 23.796752307907568 100-101 22.485735297217996 25.880455527983127 28.166974841293168 23.466834333505712 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4063.0 1 2301.0 2 596.0 3 635.5 4 579.5 5 616.5 6 944.0 7 1709.0 8 2784.0 9 3441.0 10 3521.0 11 3627.5 12 4006.5 13 4476.5 14 4913.5 15 5344.0 16 5790.5 17 6038.0 18 6245.5 19 6547.0 20 6965.0 21 7775.0 22 8987.5 23 10764.0 24 13027.0 25 16121.5 26 20914.0 27 26803.0 28 32157.0 29 37663.5 30 43789.0 31 50883.0 32 58250.5 33 66206.0 34 76230.5 35 84421.5 36 92524.5 37 102157.0 38 108806.5 39 112096.0 40 116755.0 41 125090.5 42 132065.0 43 137965.5 44 144206.0 45 148312.5 46 149150.0 47 152883.0 48 168779.5 49 171482.0 50 161044.5 51 156481.5 52 148326.5 53 143572.5 54 139969.5 55 129135.0 56 116002.0 57 102959.5 58 90754.5 59 79249.0 60 67344.0 61 54554.5 62 43410.0 63 34566.5 64 26509.5 65 19934.0 66 14439.0 67 11212.5 68 8370.0 69 5401.5 70 3864.5 71 2728.5 72 1926.5 73 1375.0 74 971.5 75 741.5 76 515.5 77 295.0 78 159.5 79 102.5 80 73.0 81 46.0 82 37.5 83 34.0 84 18.5 85 12.0 86 12.5 87 13.5 88 12.0 89 8.5 90 7.0 91 7.0 92 8.0 93 7.5 94 6.0 95 3.5 96 2.0 97 2.0 98 3.5 99 3.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2871083328484086 2 0.08923437449144543 3 0.09369732607557134 4 0.10804781127977726 5 0.11024230128523142 6 0.08570839616807525 7 0.09534935787743008 8 0.09150283607011717 9 0.0968041321507087 10-11 0.09060284859597023 12-13 0.09463429933632704 14-15 0.10359718829118761 16-17 0.09190967972281372 18-19 0.10298075851437466 20-21 0.10555743498145286 22-23 0.11814493102397369 24-25 0.13023928324504416 26-27 0.13317348898267387 28-29 0.1253694880082217 30-31 0.1180216450686111 32-33 0.1131641784273249 34-35 0.38687132792781953 36-37 0.5911561559636376 38-39 0.5740070795727008 40-41 0.5386856533613176 42-43 0.5642058461213745 44-45 0.5852754158928418 46-47 0.5744139232253973 48-49 0.5961615657513588 50-51 0.5880616784840365 52-53 0.5909712270305937 54-55 0.5814288940855289 56-57 0.5542073551414682 58-59 0.5496704319841247 60-61 0.4980752596648792 62-63 0.5713934173190137 64-65 0.6042861102097538 66-67 0.6169599064210284 68-69 0.6189448103023661 70-71 0.6153325318102423 72-73 0.6073066161161373 74-75 0.6043847389740439 76-77 0.5882466074170803 78-79 0.5653647341017829 80-81 0.5603963101006704 82-83 0.5746111807539774 84-85 0.5834507837534755 86-87 0.57463583794505 88-89 0.5869644334813093 90-91 0.6003902740202958 92-93 0.6091189196599675 94-95 0.6076888025777614 96-97 0.6047669254356679 98-99 0.6087367331983434 100-101 0.5870137478634544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4055612.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.93953846804595 #Duplication Level Percentage of deduplicated Percentage of total 1 69.81353981126207 33.46828877372457 2 14.912489005399756 14.297956806573477 3 5.965610931199191 8.579659041648572 4 3.051998596101047 5.852456164088336 5 1.7041048084308972 4.084699900867753 6 1.0852904575130562 3.1216994182170206 7 0.7606198669737629 2.552463576064409 8 0.5147133202232772 1.9740095211887563 9 0.37545411866490236 1.6199187448250139 >10 1.6626821265246998 13.38975017570791 >50 0.08811772637406465 2.934015912612976 >100 0.060066641471512576 5.345302472020692 >500 0.0037652336645134753 1.233803658863562 >1k 0.001444199117421346 1.0696937213063693 >5k 5.1578539907904734E-5 0.20624385311056995 >10k+ 5.1578539907904734E-5 0.27003825918001856 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10921 0.2692811837029775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8341 0.20566563073587907 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4982 0.12284212592328851 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.465719107251877E-5 0.0 14-15 0.0 0.0 0.0 2.465719107251877E-5 2.465719107251877E-5 16-17 0.0 0.0 0.0 6.164297768129693E-5 2.465719107251877E-5 18-19 0.0 0.0 0.0 1.2328595536259385E-4 4.931438214503754E-5 20-21 0.0 0.0 0.0 2.3424331518892832E-4 4.931438214503754E-5 22-23 0.0 0.0 0.0 3.8218646162404093E-4 4.931438214503754E-5 24-25 0.0 0.0 0.0 5.054724169866349E-4 4.931438214503754E-5 26-27 0.0 0.0 0.0 9.986162384370103E-4 4.931438214503754E-5 28-29 2.465719107251877E-5 0.0 0.0 0.003550635514442703 4.931438214503754E-5 30-31 2.465719107251877E-5 0.0 0.0 0.010947792836198334 4.931438214503754E-5 32-33 2.465719107251877E-5 0.0 0.0 0.025557178546665706 4.931438214503754E-5 34-35 2.465719107251877E-5 0.0 0.0 0.04751440719674367 4.931438214503754E-5 36-37 2.465719107251877E-5 0.0 0.0 0.0838714354331726 4.931438214503754E-5 38-39 7.397157321755632E-5 2.465719107251877E-5 0.0 0.13667481011497157 4.931438214503754E-5 40-41 7.397157321755632E-5 2.465719107251877E-5 0.0 0.19511235295684104 4.931438214503754E-5 42-43 8.63001687538157E-5 2.465719107251877E-5 0.0 0.2563608155809777 4.931438214503754E-5 44-45 9.862876429007508E-5 2.465719107251877E-5 0.0 0.3350419122933851 4.931438214503754E-5 46-47 9.862876429007508E-5 2.465719107251877E-5 0.0 0.42507764549468735 8.63001687538157E-5 48-49 9.862876429007508E-5 2.465719107251877E-5 0.0 0.5267022585000736 9.862876429007508E-5 50-51 9.862876429007508E-5 2.465719107251877E-5 0.0 0.6363651157951007 9.862876429007508E-5 52-53 9.862876429007508E-5 2.465719107251877E-5 0.0 0.7502073669769198 9.862876429007508E-5 54-55 9.862876429007508E-5 2.465719107251877E-5 0.0 0.8782768174075823 9.862876429007508E-5 56-57 9.862876429007508E-5 2.465719107251877E-5 0.0 1.0267131076641454 9.862876429007508E-5 58-59 9.862876429007508E-5 2.465719107251877E-5 0.0 1.1942833781929831 9.862876429007508E-5 60-61 9.862876429007508E-5 2.465719107251877E-5 0.0 1.3803835278128185 9.862876429007508E-5 62-63 9.862876429007508E-5 2.465719107251877E-5 0.0 1.574484935935686 9.862876429007508E-5 64-65 9.862876429007508E-5 2.465719107251877E-5 0.0 1.7789423643090116 9.862876429007508E-5 66-67 9.862876429007508E-5 2.465719107251877E-5 0.0 2.0154664696721483 1.1095735982633446E-4 68-69 9.862876429007508E-5 2.465719107251877E-5 0.0 2.2854996977028375 1.2328595536259385E-4 70-71 9.862876429007508E-5 2.465719107251877E-5 0.0 2.5878954890162076 1.4794314643511264E-4 72-73 1.2328595536259385E-4 2.465719107251877E-5 0.0 2.9002897713094846 1.4794314643511264E-4 74-75 1.4794314643511264E-4 2.465719107251877E-5 0.0 3.218909994348572 1.4794314643511264E-4 76-77 1.4794314643511264E-4 2.465719107251877E-5 0.0 3.569017450387266 1.4794314643511264E-4 78-79 1.4794314643511264E-4 2.465719107251877E-5 0.0 3.9631749782770145 1.4794314643511264E-4 80-81 1.9725752858015016E-4 2.465719107251877E-5 0.0 4.396130596319372 1.4794314643511264E-4 82-83 1.9725752858015016E-4 2.465719107251877E-5 0.0 4.8419819252926555 1.726003375076314E-4 84-85 1.9725752858015016E-4 2.465719107251877E-5 0.0 5.3071644920667955 1.726003375076314E-4 86-87 1.9725752858015016E-4 2.465719107251877E-5 0.0 5.8048822224611225 1.849289330438908E-4 88-89 1.9725752858015016E-4 2.465719107251877E-5 0.0 6.3412500998616235 1.9725752858015016E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 18645 0.0 29.37241 1 GGTATCA 12950 0.0 26.874863 1 TATCAAC 21675 0.0 25.045223 2 ATCAACG 21670 0.0 24.96364 3 CAACGCA 21725 0.0 24.8357 5 TCAACGC 21895 0.0 24.642258 4 AACGCAG 22215 0.0 24.285498 6 GTACATG 13250 0.0 24.262402 1 TACATGG 13305 0.0 23.441809 2 ACATGGG 13425 0.0 22.350927 3 GAGTACT 10030 0.0 21.964731 12-13 ACGCAGA 24415 0.0 21.670994 7 CGCAGAG 24535 0.0 21.31451 8 GCAGAGT 25195 0.0 19.70451 9 AGTACTT 10620 0.0 19.407555 12-13 GTACTTT 11385 0.0 19.268028 14-15 AGAGTAC 19765 0.0 17.922537 10-11 CATGGGG 7185 0.0 17.71928 4 CATGGGA 7430 0.0 16.689104 4 AGTACAT 10075 0.0 16.488556 2 >>END_MODULE