FastQCFastQC Report
Fri 27 May 2016
SRR938992_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938992_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4055612
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106400.26235251301159973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92750.22869544719761162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55510.1368720676435517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134500.027.2058681
GTATCAA186000.026.5637551
GTACATG130700.023.2942621
TACATGG130900.022.6919442
ATCAACG219900.022.441153
TATCAAC221650.022.3281942
CAACGCA223600.022.0485885
TCAACGC223400.022.0470874
AACGCAG229250.021.525626
ACATGGG133100.021.2473723
GAGTACT112950.020.9641912-13
AGTACTT117350.019.53114912-13
ACGCAGA252900.019.1561667
GTACTTT122750.019.0971814-15
CGCAGAG252650.018.9685338
CATGGGG68600.017.776494
GCAGAGT262150.017.5577819
CATGGGA73250.016.842354
AGAGTAC213150.016.83041610-11
AGTACAT101750.015.7622892
Produced by FastQC (version 0.11.5)