##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938992_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4055612 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18066471841981 33.0 31.0 34.0 30.0 34.0 2 32.41768640589879 34.0 31.0 34.0 31.0 34.0 3 32.51855700200118 34.0 31.0 34.0 31.0 34.0 4 36.08267161651558 37.0 35.0 37.0 35.0 37.0 5 35.99496697415828 37.0 35.0 37.0 35.0 37.0 6 36.02869554582637 37.0 35.0 37.0 35.0 37.0 7 35.97256468320934 37.0 35.0 37.0 35.0 37.0 8 35.93947177392709 37.0 35.0 37.0 35.0 37.0 9 37.629906164593656 39.0 37.0 39.0 35.0 39.0 10-11 37.55366662294125 39.0 37.0 39.0 35.0 39.0 12-13 37.47777499425487 39.0 37.0 39.0 34.0 39.0 14-15 38.76783146415387 40.0 38.0 41.0 34.0 41.0 16-17 38.75681549418435 40.0 38.0 41.0 34.5 41.0 18-19 38.841574958354 40.0 38.0 41.0 34.5 41.0 20-21 38.83675866922181 40.0 38.0 41.0 34.0 41.0 22-23 38.78233815266352 40.0 38.0 41.0 34.0 41.0 24-25 38.727263480826075 40.0 38.0 41.0 34.0 41.0 26-27 38.585587082787995 40.0 38.0 41.0 34.0 41.0 28-29 38.410608435915464 40.0 38.0 41.0 34.0 41.0 30-31 38.25704776492425 40.0 38.0 41.0 34.0 41.0 32-33 38.12347976088442 40.0 38.0 41.0 33.0 41.0 34-35 38.09951506702318 40.0 38.0 41.0 33.0 41.0 36-37 38.00827704918518 40.0 38.0 41.0 33.0 41.0 38-39 37.89579501194888 40.0 37.0 41.0 33.0 41.0 40-41 37.74936211846695 40.0 37.0 41.0 33.0 41.0 42-43 37.595563875439765 40.0 37.0 41.0 32.5 41.0 44-45 37.37092330331403 40.0 37.0 41.0 32.0 41.0 46-47 37.09859769622932 40.0 37.0 41.0 31.0 41.0 48-49 36.84754384788288 40.0 36.0 41.0 31.0 41.0 50-51 36.593963993597015 39.0 36.0 41.0 30.0 41.0 52-53 36.36989805730923 39.0 35.5 41.0 30.0 41.0 54-55 36.123369789812244 39.0 35.0 40.0 29.5 41.0 56-57 35.85825616454434 38.5 35.0 40.0 29.0 41.0 58-59 35.55754963739135 38.0 35.0 40.0 28.0 41.0 60-61 35.16359380532457 38.0 34.0 40.0 28.0 41.0 62-63 34.852238207205225 37.5 34.0 40.0 27.0 41.0 64-65 34.60128298762308 37.0 34.0 40.0 27.0 41.0 66-67 34.1634266295691 36.5 33.5 39.0 26.0 41.0 68-69 33.7603547873909 36.0 33.0 39.0 26.0 40.0 70-71 33.31189448103024 35.5 33.0 38.5 25.5 40.0 72-73 32.799286149661256 35.0 32.0 38.0 24.5 40.0 74-75 32.33002959849216 35.0 32.0 37.0 23.5 39.0 76-77 30.336520357470093 33.5 29.5 35.0 20.5 37.5 78-79 31.315374843550124 34.0 31.0 36.0 21.5 38.0 80-81 31.31632636455361 34.0 31.0 36.0 21.5 37.5 82-83 30.996658704037763 34.0 31.0 35.0 20.0 37.0 84-85 30.59758786096895 34.0 31.0 35.0 19.0 36.5 86-87 30.020225677407012 34.0 30.0 35.0 15.5 36.0 88-89 29.461689135942983 34.0 29.5 35.0 7.0 36.0 90-91 29.01366316600306 34.0 29.0 35.0 4.5 35.0 92-93 28.660544203932723 34.0 29.0 35.0 2.0 35.0 94-95 28.2768833655685 33.5 29.0 35.0 2.0 35.0 96-97 27.66768529632519 33.0 27.5 35.0 2.0 35.0 98-99 26.786046101057003 33.0 26.0 35.0 2.0 35.0 100-101 22.46752549306985 28.5 10.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2118396993363163 1101 2 0.17806290097163213 1101 3 0.11955502432751075 1101 4 0.07592923603925072 1101 5 0.19382032344544342 1101 6 0.10690718706651126 1101 7 0.10207055853077662 1101 8 0.15712708061124658 1101 9 0.17304408093205126 1101 10-11 0.2657343139573527 1101 12-13 0.2328588753379961 1101 14-15 0.21934322291958352 1101 16-17 0.2410123826967734 1101 18-19 0.31324422851302103 1101 20-21 0.26627398722830264 1101 22-23 0.2743922057813606 1101 24-25 0.30326706402824044 1101 26-27 0.29366174227460107 1101 28-29 0.3121007977305723 1101 30-31 0.25705498641758595 1101 32-33 0.270010975069475 1101 34-35 0.3233478634290421 1101 36-37 0.31944569691456337 1101 38-39 0.36704298284887926 1101 40-41 0.31341543832981245 1101 42-43 0.33712655480453435 1101 44-45 0.4480358033810532 1101 46-47 0.3639156801553156 1101 48-49 0.17676896387502694 1101 50-51 0.21779176640443865 1101 52-53 0.21015506314932253 1101 54-55 0.27297164935696827 1101 56-57 0.042455007261970934 1101 58-59 0.18136807438471436 1101 60-61 0.2803447966828472 1101 62-63 0.2570720946453733 1101 64-65 0.056647149897919746 1101 66-67 0.057066704391367296 1101 68-69 0.15683847077858815 1101 70-71 0.11241381681672635 1101 72-73 0.01768965486419205 1101 74-75 -0.006557387112962942 1101 76-77 -2.4469440798924325E-5 1101 78-79 0.07488635932866927 1101 80-81 0.2962535758086773 1101 82-83 0.18887524488494023 1101 84-85 0.11719121896489426 1101 86-87 0.2921840066362549 1101 88-89 0.4614694044079606 1101 90-91 0.49870703732073096 1101 92-93 0.5599300180436622 1101 94-95 0.4424207583470299 1101 96-97 0.4102324719328827 1101 98-99 0.4143087826901457 1101 100-101 0.9986934442496391 1102 1 0.037413957077511384 1102 2 0.05926969994153097 1102 3 0.06563770475259645 1102 4 0.07073919834088116 1102 5 0.04519907121535027 1102 6 0.06673864724132983 1102 7 0.06429349728039568 1102 8 0.08026741510100521 1102 9 0.11497541490779639 1102 10-11 0.08476369554634289 1102 12-13 0.09466193449977567 1102 14-15 0.2135845099668927 1102 16-17 0.171313752207638 1102 18-19 0.09857552466596076 1102 20-21 0.18042600508022844 1102 22-23 0.17323504821275293 1102 24-25 0.1534774550703375 1102 26-27 0.13064410079565647 1102 28-29 0.27504942558744006 1102 30-31 0.07231524927101418 1102 32-33 0.12078556306963861 1102 34-35 0.17134149782148 1102 36-37 0.18477714074872864 1102 38-39 0.22889308121414587 1102 40-41 0.25516466631939494 1102 42-43 0.16770819729045883 1102 44-45 0.2305364402563086 1102 46-47 0.21721503586864088 1102 48-49 0.0583683487554012 1102 50-51 0.11805945912741578 1102 52-53 0.14675910477409104 1102 54-55 0.15307473631836643 1102 56-57 0.15706257381868483 1102 58-59 0.052023494579493956 1102 60-61 -0.026018971698839266 1102 62-63 0.03694123677747285 1102 64-65 0.1458626399076266 1102 66-67 0.17109537423892363 1102 68-69 0.3429605314949882 1102 70-71 0.27042537259873 1102 72-73 0.24371750590142938 1102 74-75 0.29657999101755195 1102 76-77 0.20326204621088095 1102 78-79 0.07144425877662641 1102 80-81 0.1761722321721173 1102 82-83 0.258261351828839 1102 84-85 0.2800860662440492 1102 86-87 0.3611846724324259 1102 88-89 0.3999306057481924 1102 90-91 0.3520038974949209 1102 92-93 0.4315045829070989 1102 94-95 0.5180999654739047 1102 96-97 0.26097902784957583 1102 98-99 0.3262220284512516 1102 100-101 0.9545502284719234 1103 1 0.020451042767845706 1103 2 0.07622168423217346 1103 3 0.0804322662195247 1103 4 0.11760813824784577 1103 5 0.07139737638352273 1103 6 0.013072661155035803 1103 7 0.09650663944022853 1103 8 0.10482648896858393 1103 9 0.0859270224182822 1103 10-11 0.1441322323664238 1103 12-13 0.06012337987598215 1103 14-15 0.16565831135621778 1103 16-17 0.15690309917294343 1103 18-19 0.11581121423306229 1103 20-21 0.15846678219778454 1103 22-23 0.14254355478858116 1103 24-25 0.1451110686126995 1103 26-27 0.0688666488818015 1103 28-29 0.16518260549317176 1103 30-31 0.15104594140937877 1103 32-33 0.12941158839537792 1103 34-35 0.14814171777963026 1103 36-37 0.08363396937994594 1103 38-39 0.2075268288359311 1103 40-41 0.2188501872688846 1103 42-43 0.20413302880013617 1103 44-45 0.20065668743767162 1103 46-47 0.18967474839556786 1103 48-49 0.15016424931938133 1103 50-51 0.1020494252341635 1103 52-53 0.19587107467241083 1103 54-55 0.2513807191460202 1103 56-57 0.19329259980293756 1103 58-59 0.22157840180162225 1103 60-61 0.1848174293378122 1103 62-63 0.2810107001033515 1103 64-65 0.31656506335082923 1103 66-67 0.4215597812429621 1103 68-69 0.2809963867095817 1103 70-71 0.10771320039322774 1103 72-73 0.11639330694114136 1103 74-75 0.13377219811765428 1103 76-77 -0.06610599841889808 1103 78-79 0.11767141647948876 1103 80-81 0.13650417209774446 1103 82-83 0.21298948457765832 1103 84-85 0.21974550450217478 1103 86-87 0.14870136871537198 1103 88-89 0.2563115814051393 1103 90-91 0.31811632394217426 1103 92-93 0.2874683509123521 1103 94-95 0.24512341919019676 1103 96-97 0.19638896296531883 1103 98-99 0.3613059638690963 1103 100-101 0.8392119834188634 1104 1 -0.009160664811503239 1104 2 -0.044139557093835435 1104 3 0.025693284079970624 1104 4 0.04389844185857328 1104 5 0.033936146818859925 1104 6 -0.011176880992586291 1104 7 -0.03900287884866316 1104 8 0.08710348545698565 1104 9 0.0890538601285158 1104 10-11 0.09629917626480022 1104 12-13 0.05877889006635684 1104 14-15 0.06441396100254337 1104 16-17 0.013648230816009743 1104 18-19 0.0682696714805644 1104 20-21 0.10805866350901283 1104 22-23 0.0053924887066258975 1104 24-25 0.0462157949933939 1104 26-27 0.06642768186397063 1104 28-29 0.045000808695618844 1104 30-31 -0.03506710192277751 1104 32-33 0.054206688954884896 1104 34-35 0.06912009370582695 1104 36-37 -0.06469356729024867 1104 38-39 0.13013700744036782 1104 40-41 0.09857194743824493 1104 42-43 -0.010759018850109214 1104 44-45 0.18424725819897247 1104 46-47 0.11458326192951773 1104 48-49 0.08152285274361049 1104 50-51 -0.010640394481207238 1104 52-53 -0.06295295879701257 1104 54-55 0.101845796350851 1104 56-57 0.06815003549358067 1104 58-59 0.10624681586818951 1104 60-61 0.0851188558210012 1104 62-63 0.04411384710336819 1104 64-65 -0.0050769884999084525 1104 66-67 0.08361734462821602 1104 68-69 0.20138451111100863 1104 70-71 0.057394803968669805 1104 72-73 0.09102408470211287 1104 74-75 0.14416603517260995 1104 76-77 0.06476568530171889 1104 78-79 0.217593791599203 1104 80-81 0.2780605341455633 1104 82-83 0.40498303263955293 1104 84-85 0.32178374567696366 1104 86-87 0.1326815760510911 1104 88-89 0.357903395508675 1104 90-91 0.4670887183840975 1104 92-93 0.44414157032865376 1104 94-95 0.40876910025611224 1104 96-97 0.372633397328606 1104 98-99 0.4598225221633676 1104 100-101 1.1238955192153561 1105 1 -0.007943926401743795 1105 2 0.005086515235525724 1105 3 0.04061346674799893 1105 4 0.046055321692556106 1105 5 0.06805233065854566 1105 6 0.05640131286383365 1105 7 0.04151364521645462 1105 8 0.05844768389910371 1105 9 -0.0843821480678244 1105 10-11 0.021339951548917213 1105 12-13 0.012041862962995253 1105 14-15 0.1048654674109315 1105 16-17 0.04312196262112167 1105 18-19 0.054469670563165096 1105 20-21 0.05888999439200404 1105 22-23 0.06441367219572669 1105 24-25 0.15333140152451818 1105 26-27 0.15774767397430622 1105 28-29 0.13381452151049444 1105 30-31 0.07427183062647913 1105 32-33 0.02981704040539057 1105 34-35 0.16254935899058154 1105 36-37 0.19278542909714957 1105 38-39 0.22571915530344455 1105 40-41 0.2002224863797082 1105 42-43 0.10648740278696778 1105 44-45 0.1265920876832496 1105 46-47 0.08839481557104278 1105 48-49 -0.027452853771329444 1105 50-51 0.0489224499134977 1105 52-53 0.22816818622669643 1105 54-55 0.15011911296560498 1105 56-57 0.21674267385923685 1105 58-59 0.03642101758019578 1105 60-61 0.01850793230422454 1105 62-63 -0.015351261114133763 1105 64-65 0.007424166444856439 1105 66-67 0.2145874261525691 1105 68-69 0.17952522368408097 1105 70-71 0.2540714463001379 1105 72-73 0.08465639091549093 1105 74-75 0.1545209914747474 1105 76-77 -0.10275415517501685 1105 78-79 0.08371940896223506 1105 80-81 0.1023431741953118 1105 82-83 0.2415146728839268 1105 84-85 0.01561804435845815 1105 86-87 0.0413637136261471 1105 88-89 0.28917038794075367 1105 90-91 0.3814126874361641 1105 92-93 0.44670662894087343 1105 94-95 0.19410087575188584 1105 96-97 0.09224144347659546 1105 98-99 0.20460086123155818 1105 100-101 0.7011644041186393 1106 1 -0.04993242449616986 1106 2 -0.12183178043963494 1106 3 -0.11888071747565476 1106 4 -0.06769206048800669 1106 5 -0.09157919430628425 1106 6 -0.060369218798072666 1106 7 -0.10916250041992015 1106 8 -0.09626112924989627 1106 9 -0.12170939042406559 1106 10-11 -0.047768637734669994 1106 12-13 -0.0553814727676567 1106 14-15 -0.019418175046105546 1106 16-17 -0.12232289071582159 1106 18-19 -0.09386061891360242 1106 20-21 -0.07689831952594517 1106 22-23 0.04300310902684146 1106 24-25 -0.047769492608217945 1106 26-27 -0.10437323458302217 1106 28-29 -0.02015163429736333 1106 30-31 -0.036707658741917726 1106 32-33 -0.05743976077487645 1106 34-35 -0.039474674748952054 1106 36-37 -0.14577838224067108 1106 38-39 -6.990420395638353E-4 1106 40-41 -0.004916924963886515 1106 42-43 0.04846354657306051 1106 44-45 0.010644932839767307 1106 46-47 -0.010311752565506538 1106 48-49 -0.09556273424863804 1106 50-51 -0.14524556626371776 1106 52-53 0.07410700461435482 1106 54-55 -0.031080344767367762 1106 56-57 0.039551817217351015 1106 58-59 0.06958486438499278 1106 60-61 0.10679777747606778 1106 62-63 0.04124520514674401 1106 64-65 -0.04126449334921034 1106 66-67 0.010109857297329938 1106 68-69 -0.009045235327114654 1106 70-71 -0.0035821367322270703 1106 72-73 0.10651064097876883 1106 74-75 0.17612879120803626 1106 76-77 -0.06118546830446547 1106 78-79 0.22939288630962906 1106 80-81 0.2836361317245313 1106 82-83 0.19961496939368217 1106 84-85 0.20960828057626912 1106 86-87 0.21903608944992925 1106 88-89 0.27389838364911157 1106 90-91 0.5833291375951148 1106 92-93 0.593728970400857 1106 94-95 0.5904695929136992 1106 96-97 0.3758168515232505 1106 98-99 0.4501933202583963 1106 100-101 0.7686000432906859 1107 1 -0.10102661536550528 1107 2 -0.07708890677174907 1107 3 -0.052605416831504215 1107 4 -0.07144508869094324 1107 5 -0.0776817570348527 1107 6 -0.011189844654147407 1107 7 -0.1135620819464549 1107 8 -0.037773651440573985 1107 9 -0.027983372803852546 1107 10-11 0.025997498896636273 1107 12-13 -0.04113516085750035 1107 14-15 0.015322713677917932 1107 16-17 -0.02725983478531191 1107 18-19 -0.0068838506814614675 1107 20-21 0.003938487773616828 1107 22-23 -0.024635110928841186 1107 24-25 -0.05414634489228831 1107 26-27 0.07090416383734777 1107 28-29 0.02321816263081189 1107 30-31 -0.02184727841007117 1107 32-33 0.08361021792283196 1107 34-35 -0.010914376670257298 1107 36-37 0.03654459202537197 1107 38-39 -0.07549246259218734 1107 40-41 -0.07989651262136022 1107 42-43 -0.1466161826343111 1107 44-45 -0.0992440373504806 1107 46-47 -0.2184905388993812 1107 48-49 -0.18114076486985908 1107 50-51 -0.09535636713825113 1107 52-53 0.060854611203517095 1107 54-55 -0.045535025467508206 1107 56-57 -0.006071010492419759 1107 58-59 0.02557304252226089 1107 60-61 0.047813780677138595 1107 62-63 -0.03087127709498816 1107 64-65 -0.09969797640869871 1107 66-67 0.020531630194341233 1107 68-69 -0.013298650518628108 1107 70-71 0.08312676470951885 1107 72-73 0.09236109193712849 1107 74-75 0.03703809550030712 1107 76-77 -0.22136877120325238 1107 78-79 0.04432265791403722 1107 80-81 0.22712928951737865 1107 82-83 0.25166647428428135 1107 84-85 0.1767006032335452 1107 86-87 0.1919295929349616 1107 88-89 0.2691596132048488 1107 90-91 0.4278679381531596 1107 92-93 0.4826260145829657 1107 94-95 0.3380169356487208 1107 96-97 0.21860980087183535 1107 98-99 -0.15740365613624263 1107 100-101 0.3630844088388514 1108 1 0.07515574221920929 1108 2 0.0015178178100825335 1108 3 0.012573701262404313 1108 4 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-2.44680473206078 2316 96-97 -2.529575112660172 2316 98-99 -2.8112919453211056 2316 100-101 -3.7225939438762197 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 13.0 4 35.0 5 79.0 6 184.0 7 420.0 8 1026.0 9 1978.0 10 3227.0 11 5132.0 12 7353.0 13 10155.0 14 12378.0 15 16247.0 16 21111.0 17 28846.0 18 28420.0 19 20815.0 20 19310.0 21 21117.0 22 22781.0 23 25390.0 24 29539.0 25 35658.0 26 43450.0 27 53065.0 28 64771.0 29 79138.0 30 96098.0 31 118192.0 32 146030.0 33 186205.0 34 250422.0 35 356539.0 36 568210.0 37 942341.0 38 777455.0 39 62481.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.02595918351178 19.695040005387533 14.626106350254867 23.652894460845822 2 15.376816125408446 20.853671406436316 39.95816167818815 23.811350789967086 3 19.011014860395917 24.608468462959472 30.309334324881178 26.071182351763433 4 13.197317692126367 17.173413038525382 36.85604046935456 32.77322879999369 5 13.801075402708898 37.33465134726158 34.469006659192225 14.395266590837306 6 31.952736109864553 36.25694469786557 17.422697240268548 14.367621952001326 7 28.150005473896417 32.816206284033086 21.07201083338347 17.961777408687023 8 25.59512596372631 36.02082743615513 19.861983838690684 18.52206276142787 9 26.114825579961792 17.086323839657243 20.590603834883613 36.208246745497355 10-11 24.810053328572852 26.72562118861469 27.462156143141897 21.00216933967056 12-13 25.55715388947463 23.994578376826976 28.03350024607877 22.414767487619624 14-15 22.55470509157296 25.39995670146002 26.25840696879645 25.786931238170574 16-17 22.24472386981105 28.595547460113174 26.875922126107337 22.28380654396844 18-19 22.070701043393697 27.443059148656236 28.910630504101476 21.57560930384859 20-21 22.783380165558242 26.656100238385722 28.80102928978413 21.759490306271903 22-23 22.401945748261916 26.561749496746728 28.65409708818299 22.382207666808362 24-25 22.441915054990467 26.72186096697613 28.695286925869635 22.140937052163768 26-27 22.389654138512263 26.920388340896515 28.59149248004987 22.098465040541353 28-29 22.113000454678602 27.03889080119104 28.789748624868455 22.058360119261902 30-31 22.33343574286692 26.831745738990808 28.64625610142193 22.188562416720337 32-33 22.14426577295856 26.91035286413 28.618985248095726 22.326396114815715 34-35 22.56264301495246 26.88085621572573 28.45980293525861 22.096697834063203 36-37 22.54141301755946 26.769465295722373 28.46504592623937 22.2240757604788 38-39 22.390854967112382 26.9039523710589 28.6603219774617 22.04487068436702 40-41 22.572038394175234 26.713846911030448 28.569654970729953 22.144459724064365 42-43 22.355115663238518 26.8450065878232 28.324089093358463 22.475788655579827 44-45 22.463534943701234 27.039435643845106 28.129620931084094 22.367408481369562 46-47 22.412961133760167 27.12019078795207 27.791361183863707 22.675486894424058 48-49 22.6295790503189 27.13819287314453 27.75993757772425 22.472290498812324 50-51 22.514077169754398 27.269795293732123 27.703554463017294 22.512573073496185 52-53 22.69558823674454 27.306137766630535 27.510139036969022 22.488134959655902 54-55 22.393081488071346 27.08677506625387 27.709036268755494 22.81110717691929 56-57 22.494471857761543 27.430126451938698 27.513233514448622 22.56216817585114 58-59 22.592643687853762 27.21292788462783 27.59024515574801 22.604183271770392 60-61 22.67658812962834 27.0517387731652 27.810618496899593 22.461054600306866 62-63 22.57067490677116 27.037004526073993 27.796224096387917 22.596096470766927 64-65 22.37782989494417 26.978869123241893 27.776740119323733 22.8665608624902 66-67 22.280919584613525 27.27790088073167 27.540664351566747 22.900515183088057 68-69 22.191600247364846 27.141461523818727 27.74087496930131 22.926063259515114 70-71 22.24259865095576 27.10518165938951 27.592752955657495 23.059466733997237 72-73 22.342250688675346 26.954698822273926 27.56243693923383 23.140613549816898 74-75 22.215857927237614 27.236752430952464 27.335208594905037 23.21218104690488 76-77 22.2968778487823 27.014242229617423 27.550469081226392 23.138410840373883 78-79 22.267570945272457 27.10304754514643 27.455053908679794 23.17432760090132 80-81 22.067618894509632 27.34817827740918 27.400846037540077 23.183356790541108 82-83 22.19251989588748 27.21775406523109 27.365919126385858 23.223806912495572 84-85 22.212405920487463 27.047520818066424 27.212477426341575 23.527595835104542 86-87 22.124198105158822 26.923207989279014 27.51897890842786 23.433614997134303 88-89 22.149959352135575 26.88748807342744 27.423556938896944 23.53899563554004 90-91 22.01163861748801 26.978722077721578 27.506111622560642 23.50352768222977 92-93 22.074461265032255 26.919488353422366 27.410511656440505 23.595538725104866 94-95 21.990614486790154 26.885177872044956 27.264245198998328 23.85996244216656 96-97 21.92909316882293 26.732772140770166 27.35761348627616 23.980521204130746 98-99 21.77784758502539 26.752855056154285 27.240130466129404 24.229166892690916 100-101 21.87953461937245 26.402867143126613 27.78259963679895 23.93499860070199 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 693.0 1 489.0 2 351.0 3 429.0 4 408.0 5 412.5 6 691.5 7 1372.0 8 2187.5 9 2775.5 10 2773.5 11 2681.5 12 2986.0 13 3366.5 14 3669.0 15 3998.0 16 4354.5 17 4640.0 18 4994.5 19 5455.5 20 6198.5 21 7263.5 22 8732.5 23 10761.0 24 13156.5 25 16368.5 26 21156.5 27 27138.0 28 32712.5 29 38270.0 30 44312.5 31 51591.0 32 59033.5 33 66434.0 34 76041.5 35 84597.0 36 93032.0 37 102524.5 38 109550.5 39 113280.0 40 117450.5 41 125340.5 42 133291.5 43 139388.5 44 145039.5 45 149106.5 46 150260.5 47 154151.0 48 170230.5 49 173511.0 50 163186.5 51 158235.5 52 149706.5 53 144790.0 54 141005.0 55 130576.5 56 117145.0 57 103226.0 58 90445.0 59 78775.5 60 67011.5 61 53886.0 62 42549.5 63 33813.5 64 25902.5 65 19275.5 66 13773.0 67 10600.0 68 7669.0 69 4843.0 70 3404.5 71 2354.5 72 1651.5 73 1175.5 74 887.5 75 663.0 76 388.0 77 187.0 78 90.5 79 42.0 80 19.0 81 12.0 82 7.5 83 3.5 84 1.0 85 1.5 86 1.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.41069017450387263 2 0.0 3 0.0 4 0.0 5 3.6985786608778154E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001183545171480901 16-17 0.002625990849223249 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002268461578671727 36-37 0.007520443277118225 38-39 0.012686124806810908 40-41 0.002490376298324396 42-43 5.794439902041911E-4 44-45 4.1917224823281916E-4 46-47 2.465719107251877E-4 48-49 2.0958612411640958E-4 50-51 5.054724169866348E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.6027174197137204E-4 60-61 0.002330104556353024 62-63 0.0 64-65 1.6027174197137204E-4 66-67 0.001565731633104942 68-69 0.0015780602286412013 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12544345958143924 78-79 8.63001687538157E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14757328856902485 88-89 0.440981533736462 90-91 0.3383706330881751 92-93 0.0 94-95 0.0 96-97 5.547867991316723E-4 98-99 0.0 100-101 1.726003375076314E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4055612.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.91153245023645 #Duplication Level Percentage of deduplicated Percentage of total 1 67.58266398830591 31.02823670772533 2 16.039933290718285 14.728358355528808 3 6.383467749787616 8.79224360218236 4 3.221575331941204 5.916298415731992 5 1.8643590345123418 4.279779015595244 6 1.1734396339814535 3.232464710035985 7 0.7834065155045361 2.517717556081922 8 0.5522247700683462 2.028278836065379 9 0.39589762000048423 1.6358639784861246 >10 1.8338980087047743 14.158626864114954 >50 0.09753064540426928 3.1074612929465015 >100 0.06569452679864586 5.622339924210682 >500 0.004136219071922908 1.2890027815785017 >1k 0.001611513818305115 1.0352763936446063 >5k 1.074342545536716E-4 0.36564409481572835 >10k+ 5.37171272768358E-5 0.26240747125585795 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10640 0.26235251301159973 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9275 0.22869544719761162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5551 0.1368720676435517 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.465719107251877E-5 0.0 5 0.0 0.0 0.0 7.397157321755632E-5 0.0 6 0.0 0.0 0.0 7.397157321755632E-5 0.0 7 2.465719107251877E-5 2.465719107251877E-5 0.0 7.397157321755632E-5 0.0 8 2.465719107251877E-5 2.465719107251877E-5 0.0 7.397157321755632E-5 0.0 9 2.465719107251877E-5 2.465719107251877E-5 0.0 7.397157321755632E-5 0.0 10-11 4.931438214503754E-5 2.465719107251877E-5 0.0 9.862876429007508E-5 0.0 12-13 4.931438214503754E-5 2.465719107251877E-5 0.0 1.4794314643511264E-4 0.0 14-15 7.397157321755632E-5 2.465719107251877E-5 0.0 1.4794314643511264E-4 1.2328595536259385E-5 16-17 7.397157321755632E-5 2.465719107251877E-5 0.0 1.972575285801502E-4 3.6985786608778154E-5 18-19 7.397157321755632E-5 2.465719107251877E-5 0.0 2.712291017977065E-4 4.931438214503754E-5 20-21 7.397157321755632E-5 2.465719107251877E-5 0.0 3.698578660877816E-4 4.931438214503754E-5 22-23 7.397157321755632E-5 2.465719107251877E-5 0.0 4.808152259141161E-4 4.931438214503754E-5 24-25 1.2328595536259385E-4 2.465719107251877E-5 0.0 5.917725857404505E-4 4.931438214503754E-5 26-27 1.2328595536259385E-4 2.465719107251877E-5 0.0 0.0010602592161183073 4.931438214503754E-5 28-29 1.60271741971372E-4 2.465719107251877E-5 0.0 0.0036615928742690373 4.931438214503754E-5 30-31 1.726003375076314E-4 2.465719107251877E-5 0.0 0.011231350533532301 4.931438214503754E-5 32-33 1.849289330438908E-4 2.465719107251877E-5 0.0 0.02609963675026112 4.931438214503754E-5 34-35 2.0958612411640956E-4 2.465719107251877E-5 0.0 0.048623980795007016 4.931438214503754E-5 36-37 2.2191471965266895E-4 2.465719107251877E-5 0.0 0.08569606757253899 4.931438214503754E-5 38-39 2.2191471965266895E-4 2.465719107251877E-5 0.0 0.13927614377312228 4.931438214503754E-5 40-41 2.2191471965266895E-4 2.465719107251877E-5 0.0 0.19858901689806618 4.931438214503754E-5 42-43 2.2191471965266895E-4 2.465719107251877E-5 0.0 0.2606634954231322 4.931438214503754E-5 44-45 2.465719107251877E-4 2.465719107251877E-5 0.0 0.3404048513516579 6.164297768129693E-5 46-47 2.712291017977065E-4 2.465719107251877E-5 0.0 0.4320063161860651 7.397157321755632E-5 48-49 2.712291017977065E-4 2.465719107251877E-5 0.0 0.5347281741941783 7.397157321755632E-5 50-51 2.712291017977065E-4 2.465719107251877E-5 0.0 0.6454266335142513 7.397157321755632E-5 52-53 2.712291017977065E-4 2.465719107251877E-5 0.0 0.7604647584630877 7.397157321755632E-5 54-55 2.712291017977065E-4 2.465719107251877E-5 0.0 0.8902971980554353 7.397157321755632E-5 56-57 3.20543483942744E-4 2.465719107251877E-5 0.0 1.0406197634290462 7.397157321755632E-5 58-59 3.20543483942744E-4 2.465719107251877E-5 0.0 1.2091763216007843 7.397157321755632E-5 60-61 3.20543483942744E-4 2.465719107251877E-5 1.2328595536259385E-5 1.3968791886403333 7.397157321755632E-5 62-63 3.20543483942744E-4 2.465719107251877E-5 2.465719107251877E-5 1.5933353585106267 7.397157321755632E-5 64-65 3.20543483942744E-4 2.465719107251877E-5 2.465719107251877E-5 1.7995311188545648 7.397157321755632E-5 66-67 3.452006750152628E-4 2.465719107251877E-5 2.465719107251877E-5 2.037744241806169 7.397157321755632E-5 68-69 3.452006750152628E-4 2.465719107251877E-5 2.465719107251877E-5 2.309392515852108 7.397157321755632E-5 70-71 3.452006750152628E-4 2.465719107251877E-5 2.465719107251877E-5 2.614525255374528 7.397157321755632E-5 72-73 3.452006750152628E-4 2.465719107251877E-5 2.465719107251877E-5 2.9300016865518694 9.862876429007508E-5 74-75 3.452006750152628E-4 2.465719107251877E-5 2.465719107251877E-5 3.252123230723255 9.862876429007508E-5 76-77 3.452006750152628E-4 2.465719107251877E-5 2.465719107251877E-5 3.60601556559158 9.862876429007508E-5 78-79 3.452006750152628E-4 2.465719107251877E-5 2.465719107251877E-5 4.002342926295711 9.862876429007508E-5 80-81 3.698578660877816E-4 2.465719107251877E-5 2.465719107251877E-5 4.437875220805147 9.862876429007508E-5 82-83 3.698578660877816E-4 2.465719107251877E-5 2.465719107251877E-5 4.885662139277623 9.862876429007508E-5 84-85 3.698578660877816E-4 2.465719107251877E-5 2.465719107251877E-5 5.352570709426839 9.862876429007508E-5 86-87 3.698578660877816E-4 2.465719107251877E-5 2.465719107251877E-5 5.853087031007897 9.862876429007508E-5 88-89 3.698578660877816E-4 3.6985786608778154E-5 2.465719107251877E-5 6.392438428528173 9.862876429007508E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13450 0.0 27.205868 1 GTATCAA 18600 0.0 26.563755 1 GTACATG 13070 0.0 23.294262 1 TACATGG 13090 0.0 22.691944 2 ATCAACG 21990 0.0 22.44115 3 TATCAAC 22165 0.0 22.328194 2 CAACGCA 22360 0.0 22.048588 5 TCAACGC 22340 0.0 22.047087 4 AACGCAG 22925 0.0 21.52562 6 ACATGGG 13310 0.0 21.247372 3 GAGTACT 11295 0.0 20.96419 12-13 AGTACTT 11735 0.0 19.531149 12-13 ACGCAGA 25290 0.0 19.156166 7 GTACTTT 12275 0.0 19.09718 14-15 CGCAGAG 25265 0.0 18.968533 8 CATGGGG 6860 0.0 17.77649 4 GCAGAGT 26215 0.0 17.557781 9 CATGGGA 7325 0.0 16.84235 4 AGAGTAC 21315 0.0 16.830416 10-11 AGTACAT 10175 0.0 15.762289 2 >>END_MODULE