FastQCFastQC Report
Fri 27 May 2016
SRR938991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3772910
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91520.24257138389200908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73270.19420023271162049No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43920.11640881971740646No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82700.036.7878341
GTATCAA126500.033.8053931
ATCAACG152650.027.6796343
TATCAAC155000.027.3516582
TCAACGC156850.027.0290494
CAACGCA158050.026.763895
AACGCAG162750.026.036076
GTACATG118550.022.9806371
ACGCAGA181700.022.8777317
CGCAGAG184200.022.207398
TACATGG121500.021.949492
ACATGGG122800.021.0999413
GCAGAGT196200.019.980169
GAGTACT96250.019.23374212-13
CATGGGG72600.018.0732154
AGAGTAC162750.017.8322910-11
CAGAGTA182000.017.78012310-11
GTACTTT103100.017.65804914-15
AGTACTT104450.017.11182412-13
AGTACAT89750.017.1002672
Produced by FastQC (version 0.11.5)