FastQCFastQC Report
Fri 27 May 2016
SRR938991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3772910
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91920.2436315735069217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82870.2196447834695235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.12377713754104921No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86550.035.060931
GTATCAA130150.032.209911
ATCAACG159250.026.0452213
CAACGCA163100.025.4886115
TATCAAC164750.025.2621442
TCAACGC164200.025.2600574
AACGCAG168150.024.7513456
GTACATG124900.021.5876331
ACGCAGA190750.021.4953867
CGCAGAG190600.021.2633198
TACATGG125100.021.053862
GTGTAGG17900.020.2260741
ACATGGG126750.019.9560833
GAGTACT96250.019.77307712-13
GCAGAGT204650.019.1544699
GTACTTT101050.018.55202714-15
AGTACTT103250.018.17971212-13
TATACCG2352.5527497E-618.1748455
AGTACAT98350.018.1430172
AGAGTAC173050.017.92274710-11
Produced by FastQC (version 0.11.5)