FastQCFastQC Report
Fri 27 May 2016
SRR938990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3779930
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91220.24132722034535029No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73350.19405121258859293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44220.11698629339696766No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88300.038.3939361
GTATCAA134050.032.5061871
ATCAACG158450.027.0022013
TATCAAC164100.026.2455582
CAACGCA163700.026.1362175
TCAACGC164450.026.103364
AACGCAG167750.025.5028466
GTACATG126300.023.3133961
ACGCAGA188900.022.3468157
TACATGG127550.022.3006232
CGCAGAG189550.022.0707448
GAGTACT92900.021.52369112-13
ACATGGG129600.020.8888243
GTACTTT99950.019.69799614-15
GCAGAGT202250.019.5154029
CATGGGG77000.018.1938234
AGTACTT98250.018.0876912-13
CAGAGTA190200.017.8778910-11
AGAGTAC170850.017.5343110-11
AGTACAT97500.017.4266072
Produced by FastQC (version 0.11.5)