##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938990_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3779930 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.714680695145148 33.0 31.0 34.0 30.0 34.0 2 31.791305923654672 33.0 31.0 34.0 30.0 34.0 3 31.841471138354414 34.0 31.0 34.0 30.0 34.0 4 35.37171217456408 37.0 35.0 37.0 33.0 37.0 5 35.39848991912549 37.0 35.0 37.0 33.0 37.0 6 35.46860947160397 37.0 35.0 37.0 33.0 37.0 7 35.36919069929866 37.0 35.0 37.0 33.0 37.0 8 35.34600138097795 37.0 35.0 37.0 33.0 37.0 9 36.94529343135984 39.0 37.0 39.0 33.0 39.0 10-11 36.83195217900861 39.0 37.0 39.0 33.0 39.0 12-13 36.70315958232031 39.0 37.0 39.0 32.0 39.0 14-15 37.92136613640994 40.0 38.0 41.0 33.0 41.0 16-17 37.83602804813846 40.0 38.0 41.0 32.0 41.0 18-19 37.75128970642314 40.0 38.0 41.0 32.0 41.0 20-21 37.618749289007994 40.0 37.5 41.0 32.0 41.0 22-23 37.7013256859254 40.0 38.0 41.0 32.0 41.0 24-25 37.64220474982341 40.0 38.0 41.0 32.0 41.0 26-27 37.558026603667265 40.0 37.0 41.0 32.0 41.0 28-29 37.42250729510864 40.0 37.0 41.0 31.0 41.0 30-31 37.248575238165785 40.0 37.0 41.0 31.0 41.0 32-33 37.07468894926626 40.0 36.5 41.0 30.5 41.0 34-35 36.76208064699611 40.0 36.0 41.0 30.0 41.0 36-37 36.505684629080434 39.5 36.0 41.0 30.0 41.0 38-39 36.30349490599032 39.0 35.5 41.0 30.0 41.0 40-41 36.150242729362716 39.0 35.0 41.0 29.0 41.0 42-43 35.948472855317426 39.0 35.0 41.0 28.5 41.0 44-45 35.91571232800607 39.0 35.0 41.0 28.0 41.0 46-47 35.72263772080435 39.0 35.0 41.0 27.0 41.0 48-49 35.56589764889826 39.0 35.0 41.0 26.5 41.0 50-51 34.40747394793026 38.0 34.0 40.0 24.5 40.5 52-53 34.54366178738759 38.0 34.0 39.5 25.0 40.5 54-55 35.03175336580307 39.0 35.0 40.0 26.0 41.0 56-57 34.92378364149601 38.5 34.5 40.5 25.5 41.0 58-59 34.708966964996705 38.0 34.0 40.0 25.0 41.0 60-61 34.43642289672031 38.0 34.0 40.0 24.5 41.0 62-63 34.065447772842354 37.5 34.0 40.0 23.0 41.0 64-65 33.6512322979526 37.0 33.0 40.0 22.0 41.0 66-67 33.27000751336665 36.0 33.0 39.0 22.0 41.0 68-69 32.871465873706654 36.0 32.5 39.0 20.5 41.0 70-71 32.46309071861119 35.0 32.0 38.5 20.0 40.0 72-73 32.02313310034842 35.0 32.0 37.5 18.5 40.0 74-75 31.55211577992185 35.0 31.0 37.0 17.5 39.0 76-77 31.13927757921443 35.0 31.0 36.5 13.5 39.0 78-79 30.715150412838334 35.0 31.0 36.0 9.5 38.5 80-81 30.294361932628384 34.0 30.0 35.5 7.0 37.0 82-83 29.917663290060926 34.0 30.0 35.0 4.5 37.0 84-85 29.567079284537016 34.0 30.0 35.0 2.0 36.5 86-87 29.168201395263935 34.0 29.0 35.0 2.0 36.0 88-89 28.764249734783448 34.0 29.0 35.0 2.0 36.0 90-91 28.345180730860093 34.0 29.0 35.0 2.0 35.0 92-93 27.92175966221597 34.0 27.5 35.0 2.0 35.0 94-95 27.4467200450802 34.0 27.0 35.0 2.0 35.0 96-97 26.8528716140246 33.0 25.5 35.0 2.0 35.0 98-99 26.12349726053128 33.0 24.5 35.0 2.0 35.0 100-101 23.486829385729365 30.0 10.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2902918234646812 1101 2 0.29140574276682685 1101 3 0.2899570261689597 1101 4 0.2242661203109435 1101 5 0.04186070347828519 1101 6 0.18360225678462427 1101 7 0.22090679004157465 1101 8 0.23066307889295956 1101 9 0.22860570777138634 1101 10-11 0.3437189976253805 1101 12-13 0.41167288089712883 1101 14-15 0.1815770916919135 1101 16-17 0.40955583139571416 1101 18-19 0.380736010207265 1101 20-21 0.446963812496719 1101 22-23 0.44180031627491445 1101 24-25 0.5140361129229589 1101 26-27 0.46723063288734323 1101 28-29 0.3773680874509253 1101 30-31 0.43518884267559343 1101 32-33 0.34018657418320686 1101 34-35 0.36999972141415327 1101 36-37 0.30156774335571157 1101 38-39 0.3687176941599617 1101 40-41 0.31666514638225607 1101 42-43 0.17978600910465303 1101 44-45 0.2208022045557314 1101 46-47 0.31254421554651657 1101 48-49 0.4241407937444208 1101 50-51 0.33700660620434064 1101 52-53 0.261929861449282 1101 54-55 0.33791706867603466 1101 56-57 0.2920643632301889 1101 58-59 0.2314677964118843 1101 60-61 0.2964028164471344 1101 62-63 0.3477417338874105 1101 64-65 0.30645923363630345 1101 66-67 0.33649790041520333 1101 68-69 0.3502811899969487 1101 70-71 0.27405121303225144 1101 72-73 0.07269744343187057 1101 74-75 0.09254700087066325 1101 76-77 0.015702948716409537 1101 78-79 -0.12013762679145401 1101 80-81 -0.21148983941444044 1101 82-83 -0.2889901439670304 1101 84-85 -0.2947962874729768 1101 86-87 -0.29877740975629763 1101 88-89 -0.32495141503773084 1101 90-91 -0.3558864368010113 1101 92-93 -0.1646299090924721 1101 94-95 -0.15727400401535618 1101 96-97 -0.29632508467383545 1101 98-99 -0.43442125537348986 1101 100-101 -0.09421968496760869 1102 1 0.31917155010540554 1102 2 0.22715288791995647 1102 3 0.2300111461353005 1102 4 0.2568623995321886 1102 5 0.3356386475410602 1102 6 0.3776625469740438 1102 7 0.3783403924002826 1102 8 0.26472857722797016 1102 9 0.4442133019685883 1102 10-11 0.31482482117738186 1102 12-13 0.424836189200704 1102 14-15 0.4811811048229018 1102 16-17 0.5077430486922268 1102 18-19 0.5324387879263455 1102 20-21 0.5803136258611445 1102 22-23 0.6060186469586029 1102 24-25 0.5259481922160418 1102 26-27 0.5723379094995309 1102 28-29 0.6221868216105122 1102 30-31 0.6529488366595047 1102 32-33 0.6118712290472246 1102 34-35 0.523569787131251 1102 36-37 0.5335674785979521 1102 38-39 0.5002446565680856 1102 40-41 0.48681131628216434 1102 42-43 0.48045868647681544 1102 44-45 0.6852118631176651 1102 46-47 0.5951756093531699 1102 48-49 0.6876390889779174 1102 50-51 0.6129716668811085 1102 52-53 0.621722449226418 1102 54-55 0.741144378749631 1102 56-57 0.6090831908629397 1102 58-59 0.6266638103781688 1102 60-61 0.5671639266398145 1102 62-63 0.6036646011269298 1102 64-65 0.5780056053984595 1102 66-67 0.5771443692552012 1102 68-69 0.49162050378958355 1102 70-71 0.49257347319342415 1102 72-73 0.44819199973888857 1102 74-75 0.4292222276238782 1102 76-77 0.39884953253336874 1102 78-79 0.27420988827674364 1102 80-81 0.16913082486650666 1102 82-83 -0.010580579872655704 1102 84-85 -0.0264285625625007 1102 86-87 0.18624140296052616 1102 88-89 0.049907660497783723 1102 90-91 -0.07702007234766128 1102 92-93 -0.23754379197010422 1102 94-95 -0.1970244915798176 1102 96-97 -0.395632084346925 1102 98-99 -0.25061473487465236 1102 100-101 0.12750779104570853 1103 1 0.03808531316199293 1103 2 0.21146536055584875 1103 3 0.2062278293788431 1103 4 0.23070538076945013 1103 5 0.20818323646934545 1103 6 0.15774865269239058 1103 7 0.21148005634147182 1103 8 0.1939427721008684 1103 9 0.25639620027651944 1103 10-11 0.26032165521937145 1103 12-13 0.3376055531761111 1103 14-15 0.35801702934028157 1103 16-17 0.399887848807019 1103 18-19 0.40401927120250747 1103 20-21 0.37874242031976735 1103 22-23 0.4002459398100271 1103 24-25 0.4430211558053827 1103 26-27 0.44890402547060404 1103 28-29 0.3237032989410764 1103 30-31 0.3404780403686374 1103 32-33 0.3595422907315893 1103 34-35 0.3659928579694878 1103 36-37 0.3761553472698651 1103 38-39 0.4136309009925583 1103 40-41 0.3429927833783921 1103 42-43 0.27937007031258787 1103 44-45 0.38732805126252146 1103 46-47 0.3955064007629616 1103 48-49 0.2133463246299243 1103 50-51 0.19221259732814389 1103 52-53 0.20291887843294631 1103 54-55 0.31114988529677845 1103 56-57 0.2715378293010957 1103 58-59 0.2357121465792389 1103 60-61 0.22902038726677176 1103 62-63 0.13330705574697532 1103 64-65 0.004123900502783329 1103 66-67 0.09668678622468718 1103 68-69 0.06932107271769894 1103 70-71 -9.248665661942823E-5 1103 72-73 0.0929634473721066 1103 74-75 0.10394150409546654 1103 76-77 -0.019493524127650375 1103 78-79 0.0458289112509398 1103 80-81 0.14716840919700758 1103 82-83 0.09864665779065618 1103 84-85 0.03225678916360053 1103 86-87 0.19743753783046003 1103 88-89 0.14807228786152038 1103 90-91 0.04259418114727609 1103 92-93 -0.08874529848291601 1103 94-95 -0.19529649048096331 1103 96-97 -0.3599712128407937 1103 98-99 -0.41417374874894364 1103 100-101 -0.0780749770182787 1104 1 0.13982493694912534 1104 2 0.17230605720186887 1104 3 0.13710952142076493 1104 4 0.0908026184664692 1104 5 0.17388903461281302 1104 6 0.21334341219291275 1104 7 0.14470201200750665 1104 8 0.10033375887309859 1104 9 0.19296683829685435 1104 10-11 0.1780682471822317 1104 12-13 0.25559944399822143 1104 14-15 0.25827586217544507 1104 16-17 0.1898729878994132 1104 18-19 0.3223028899851528 1104 20-21 0.3113067249159869 1104 22-23 0.3489043530719229 1104 24-25 0.364647215116058 1104 26-27 0.2330297363112308 1104 28-29 0.27275261135987705 1104 30-31 0.35665421181774093 1104 32-33 0.36353334508403634 1104 34-35 0.27666328678524366 1104 36-37 0.27488497096202735 1104 38-39 0.21972094749301618 1104 40-41 0.3119026679497878 1104 42-43 0.32458172413155495 1104 44-45 0.29386022584740346 1104 46-47 0.40922934302047764 1104 48-49 0.21862805845762523 1104 50-51 0.2654705380799598 1104 52-53 0.15718145605752198 1104 54-55 0.1744683648706129 1104 56-57 0.11394531011838893 1104 58-59 0.18925492292517987 1104 60-61 0.09257352887366466 1104 62-63 0.10994056848749523 1104 64-65 0.08408636683138582 1104 66-67 0.1897181816152056 1104 68-69 0.17042598318923297 1104 70-71 0.12901471161656985 1104 72-73 0.10073869506156541 1104 74-75 0.15473369524147884 1104 76-77 0.09993983905412307 1104 78-79 0.0626529306562027 1104 80-81 0.023965327588232554 1104 82-83 -0.0378272357518199 1104 84-85 0.0719379551459376 1104 86-87 0.06995298462727817 1104 88-89 0.03417507994146263 1104 90-91 0.14110253018346341 1104 92-93 0.2263070915008747 1104 94-95 0.23785362674283306 1104 96-97 0.1661775971990629 1104 98-99 0.05691912840058322 1104 100-101 0.4729948030764355 1105 1 0.00277502520719608 1105 2 0.12940170719912913 1105 3 0.13691313438791397 1105 4 0.0857817914793273 1105 5 0.2035264743400873 1105 6 0.23728577776685 1105 7 0.16342723846115348 1105 8 0.13464411644655172 1105 9 0.15516248427200452 1105 10-11 0.07394975195030895 1105 12-13 0.17105752274432717 1105 14-15 0.18410215762018822 1105 16-17 0.23921586655132643 1105 18-19 0.25452483399656955 1105 20-21 0.3471127547468953 1105 22-23 0.3300674137046613 1105 24-25 0.3260108751411934 1105 26-27 0.30972610046222115 1105 28-29 0.2878576353551239 1105 30-31 0.326736946783555 1105 32-33 0.20496995293021314 1105 34-35 0.23893034963142412 1105 36-37 0.25711628867183123 1105 38-39 0.2247032011308363 1105 40-41 0.2366914900972219 1105 42-43 0.11322332581087124 1105 44-45 0.10535582545214339 1105 46-47 0.37417354610893483 1105 48-49 0.33327651630634847 1105 50-51 0.3042912811344962 1105 52-53 0.35840371442301233 1105 54-55 0.3972562712942178 1105 56-57 0.4688477993128046 1105 58-59 0.2921200839030078 1105 60-61 0.3998038522898568 1105 62-63 0.30814414394487244 1105 64-65 0.2984192572328155 1105 66-67 0.21861115570057876 1105 68-69 0.12731917927733605 1105 70-71 0.09718671759625863 1105 72-73 0.3955182154143202 1105 74-75 0.3289389175936144 1105 76-77 0.24041296702394277 1105 78-79 0.2938815098080063 1105 80-81 0.2512448571162018 1105 82-83 0.21027901877071642 1105 84-85 0.262146586033964 1105 86-87 0.2744093749092791 1105 88-89 0.3437953406718357 1105 90-91 0.18031428716057363 1105 92-93 0.29295075397073944 1105 94-95 0.3770467561627129 1105 96-97 0.30803183832384207 1105 98-99 0.21540080497360847 1105 100-101 0.5106284232182254 1106 1 0.20685857583114142 1106 2 0.24504480278516638 1106 3 0.23499293642548125 1106 4 0.1425556015850944 1106 5 0.19662188917361334 1106 6 0.24412457653193087 1106 7 0.23504181961268245 1106 8 0.174421721983272 1106 9 0.22973249947136054 1106 10-11 0.20440178175221035 1106 12-13 0.21483035902193848 1106 14-15 0.21586828353914456 1106 16-17 0.2823166688758718 1106 18-19 0.301359069239858 1106 20-21 0.3359412694145263 1106 22-23 0.3133934022254792 1106 24-25 0.3144735941040935 1106 26-27 0.40880209657797906 1106 28-29 0.30773554657641 1106 30-31 0.325168928270692 1106 32-33 0.2830418737131879 1106 34-35 0.3522296862815679 1106 36-37 0.17483014055596868 1106 38-39 0.20976633677008039 1106 40-41 0.2735068498158739 1106 42-43 0.22593852584268603 1106 44-45 0.3747583880328236 1106 46-47 0.21345011862430852 1106 48-49 0.24234929158400575 1106 50-51 0.21056583947861895 1106 52-53 0.09006101958034662 1106 54-55 -0.05379835465909366 1106 56-57 0.04838199225025619 1106 58-59 0.25200309193120063 1106 60-61 0.28178468739248785 1106 62-63 0.19423018162991923 1106 64-65 0.17398030850679191 1106 66-67 0.2422777589607179 1106 68-69 0.13359595235745303 1106 70-71 0.12989862320829104 1106 72-73 0.08317640238627177 1106 74-75 0.04966821267156263 1106 76-77 0.0732566057156987 1106 78-79 0.0467210162686591 1106 80-81 0.01486958146717754 1106 82-83 0.17609467798871137 1106 84-85 0.15878678781972155 1106 86-87 0.2660663041443101 1106 88-89 0.41626849628669405 1106 90-91 0.15070854877961537 1106 92-93 0.2194584919596032 1106 94-95 0.35132420036611833 1106 96-97 0.31815933790657525 1106 98-99 0.2882122177995967 1106 100-101 0.7365934926061222 1107 1 0.0473643002830606 1107 2 -0.0015297470226407484 1107 3 0.03950558505788493 1107 4 0.09019474916117076 1107 5 -0.016144651047838465 1107 6 0.04223962886625543 1107 7 -0.035812319436516304 1107 8 0.12767475600875855 1107 9 0.07244329519283355 1107 10-11 0.08790503361031199 1107 12-13 0.10994047563337261 1107 14-15 0.03182840195749037 1107 16-17 0.12613093236642925 1107 18-19 0.16717539518514002 1107 20-21 0.11029058947273995 1107 22-23 0.09773383976071415 1107 24-25 0.11250640351101282 1107 26-27 0.1519666122610701 1107 28-29 0.10796245674906402 1107 30-31 0.2059605655631742 1107 32-33 0.24443684999681636 1107 34-35 0.07760348648130133 1107 36-37 0.09072950532794977 1107 38-39 0.14801308390814683 1107 40-41 0.1980375273599293 1107 42-43 0.23993057889575908 1107 44-45 0.37500827173114004 1107 46-47 0.26965308527798726 1107 48-49 0.23428098793652197 1107 50-51 0.2502956624992976 1107 52-53 0.2558390276351261 1107 54-55 0.3725357518199033 1107 56-57 0.46790459894521774 1107 58-59 0.3585053167207022 1107 60-61 0.21489989173640112 1107 62-63 0.09211999446308283 1107 64-65 0.3093634243134389 1107 66-67 0.20045766059616454 1107 68-69 0.23566146877993788 1107 70-71 0.2868863537394404 1107 72-73 0.13362931927832022 1107 74-75 0.2983220673194147 1107 76-77 0.2882286832738643 1107 78-79 0.3383481986818957 1107 80-81 0.24890782105839193 1107 82-83 0.3344210609461946 1107 84-85 0.22653656826624058 1107 86-87 0.2429178240395018 1107 88-89 0.4645222215256233 1107 90-91 0.427293126918574 1107 92-93 0.44131918549047455 1107 94-95 0.5616821772273894 1107 96-97 0.5430762488880383 1107 98-99 0.49298710449508576 1107 100-101 0.6476309882107536 1108 1 0.37590330179099496 1108 2 0.327063877225207 1108 3 0.25818205863703625 1108 4 0.30997006956130946 1108 5 0.25885600268176034 1108 6 0.32631579272285194 1108 7 0.3456461658284198 1108 8 0.3593190457533382 1108 9 0.4723700774776347 1108 10-11 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2316 92-93 -3.0041494148298504 2316 94-95 -3.20115675013335 2316 96-97 -3.4378485792173734 2316 98-99 -3.697912743423867 2316 100-101 -3.9495247523762202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13556.0 3 3846.0 4 3963.0 5 4314.0 6 5084.0 7 6104.0 8 7490.0 9 8916.0 10 10884.0 11 13413.0 12 16995.0 13 23066.0 14 21950.0 15 19895.0 16 23531.0 17 29716.0 18 26826.0 19 21604.0 20 22126.0 21 23947.0 22 26209.0 23 29845.0 24 33784.0 25 39075.0 26 44749.0 27 52636.0 28 62375.0 29 73654.0 30 87860.0 31 107376.0 32 133619.0 33 171352.0 34 228243.0 35 325568.0 36 502741.0 37 767445.0 38 680540.0 39 105577.0 40 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34972807926783 18.70229382295956 14.005688593920828 23.94228950385178 2 15.421430322775045 19.660464227102807 40.52139891958188 24.396706530540268 3 19.426862990523887 23.689272931451438 29.310992654934438 27.572871423090234 4 13.189996962404527 15.996025437412422 36.35286742921701 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28.17835153459582 22.800034753162485 26-27 22.815506269470617 26.384715871546543 28.07654779190743 22.72323006707541 28-29 22.68081882822857 26.40730307689659 28.237588384791522 22.674289710083315 30-31 22.84855631074081 26.251670562659807 28.07032598683043 22.829447139768952 32-33 22.67505606645981 26.359657997243552 28.056827078007856 22.90845885828878 34-35 23.017616977690544 26.372619718953196 27.91626461702456 22.6934986863317 36-37 23.066916348045268 26.200059289126564 27.917115504159817 22.815908858668347 38-39 22.878734545458414 26.41859581543069 27.996223516524978 22.70644612258592 40-41 23.012820708880373 26.290624809257196 27.924560066479227 22.7719944153832 42-43 22.857927662251807 26.320993270628733 27.794498117132825 23.026580949986634 44-45 22.917137600988386 26.484924935009403 27.706837347678388 22.891100116323827 46-47 22.969048718459355 26.60311477693183 27.413956113117894 23.013880391490922 48-49 23.09364170894798 26.584809170899455 27.360981949312453 22.96056717084011 50-51 23.014998153919844 26.720756260872747 27.28740640706234 22.976839178145074 52-53 23.14254602121649 26.774666882393888 27.13377021644395 22.94901687994567 54-55 22.999539145247475 26.603106671910265 27.243686795447008 23.15366738739525 56-57 22.923496439207973 26.941477561104847 27.154404199106423 22.98062180058075 58-59 23.01049647699115 26.645727709233636 27.211175317352748 23.132600496422462 60-61 23.061814857295985 26.542746205417167 27.44873529856274 22.946703638724106 62-63 22.989610316705555 26.553972863615304 27.4938106604166 22.96260615926254 64-65 22.87702995246123 26.45462737873514 27.47606427288991 23.19227839591372 66-67 22.820237257886024 26.640292849190782 27.27243931637468 23.267030576548507 68-69 22.687915835234655 26.7071512447424 27.43056863361754 23.17436428640541 70-71 22.804253077063432 26.460316685034847 27.35905091110463 23.376379326797093 72-73 22.860601273150305 26.492124385262372 27.242970605973344 23.404303735613983 74-75 22.79714474790987 26.71189172494013 27.115912477296206 23.375051049853802 76-77 22.906574219518163 26.50250787194246 27.209366128015493 23.381551780523885 78-79 22.834925115790487 26.529911583012456 27.246316779141633 23.388846522055427 80-81 22.728593178004957 26.72827714045608 27.179708048343105 23.36342163319586 82-83 22.884469972100856 26.632589586695676 27.153949221745265 23.328991219458203 84-85 22.944224595970777 26.456836140392703 27.057890809931713 23.541048453704807 86-87 22.787582070618047 26.343135202039324 27.42715779508905 23.442124932253577 88-89 22.91288169631245 26.26820760612564 27.346211512713975 23.47269918484794 90-91 22.81276883678875 26.369980632107854 27.352467027677257 23.46478350342614 92-93 22.784674522234933 26.304961863298743 27.2944334322765 23.61593018218983 94-95 22.846571285647617 26.22777078906055 27.258685316494407 23.666972608797426 96-97 22.84530286017234 26.088682987873668 27.317138278701456 23.74887587325253 98-99 22.671868231749553 26.080175343224116 27.278540350167447 23.969416074858888 100-101 22.990141782967548 25.646265698873016 27.62623793168367 23.73735458647577 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3378.0 1 1901.5 2 462.5 3 494.5 4 459.5 5 480.5 6 752.0 7 1456.5 8 2343.0 9 2898.5 10 2967.5 11 2987.5 12 3276.5 13 3676.0 14 4128.5 15 4537.5 16 4934.0 17 5247.5 18 5475.0 19 5822.0 20 6220.5 21 6774.5 22 7851.5 23 9557.5 24 11587.0 25 14286.0 26 18230.5 27 22842.0 28 27283.5 29 31763.0 30 36794.5 31 43196.5 32 49611.5 33 56162.5 34 63013.5 35 69724.0 36 78167.5 37 86940.0 38 92638.5 39 96653.0 40 101322.5 41 106783.0 42 112525.5 43 119420.0 44 127350.0 45 133047.5 46 136313.5 47 140567.0 48 149526.0 49 152089.5 50 147939.0 51 146414.0 52 144169.5 53 143151.0 54 140820.0 55 132240.5 56 120702.0 57 109272.0 58 99196.0 59 88635.5 60 75914.5 61 62721.0 62 50856.0 63 40500.0 64 30971.5 65 23029.5 66 16573.0 67 12426.5 68 9380.0 69 6476.5 70 4508.5 71 3015.5 72 2108.5 73 1504.5 74 1064.0 75 722.5 76 457.0 77 255.5 78 144.0 79 104.0 80 83.0 81 59.5 82 57.0 83 48.0 84 30.0 85 25.0 86 23.0 87 17.0 88 16.0 89 15.0 90 10.0 91 9.5 92 6.5 93 3.5 94 3.0 95 4.5 96 5.0 97 4.0 98 2.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28130150558343675 2 0.08582169511075602 3 0.08944610085371951 4 0.10362625762910954 5 0.10558396584063727 6 0.08137716836026064 7 0.09108634286878328 8 0.08804395848600371 9 0.09256785178561508 10-11 0.08613916130721998 12-13 0.09002812221390344 14-15 0.09861293727661624 16-17 0.08750162040037779 18-19 0.09820287677285029 20-21 0.10054418997177197 22-23 0.11235657803186831 24-25 0.1252404145050305 26-27 0.12832248216236808 28-29 0.11997576674700325 30-31 0.11259467767921627 32-33 0.10725066337207304 34-35 0.37909432185252107 36-37 0.5839790683954466 38-39 0.566333238975325 40-41 0.5280124235104883 42-43 0.5564917868849423 44-45 0.5790186590756972 46-47 0.5668491215445788 48-49 0.5889394777151957 50-51 0.5811615559018288 52-53 0.5846140007883743 54-55 0.5739788832068319 56-57 0.5464122351472117 58-59 0.5404994272380705 60-61 0.4854719531843182 62-63 0.5621664951467356 64-65 0.5972597376141886 66-67 0.6097335135835849 68-69 0.6113473000822767 70-71 0.6079345384702891 72-73 0.5988470685965084 74-75 0.5971935988232586 76-77 0.5810425060781549 78-79 0.5580394345927041 80-81 0.5524705483963989 82-83 0.5659364062297451 84-85 0.5763598796803115 86-87 0.5669152603355089 88-89 0.579891691115973 90-91 0.5931988158510872 92-93 0.601135470762686 94-95 0.6001037056241781 96-97 0.5970745489995847 98-99 0.6012677483445461 100-101 0.5793096697557891 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3779930.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.83073241281896 #Duplication Level Percentage of deduplicated Percentage of total 1 73.27599537225203 38.712245037944065 2 14.2294599784571 15.035055850015674 3 5.092973935088355 8.071966294503435 4 2.4190481841591787 5.112003492441166 5 1.3209519060080444 3.4893428338257086 6 0.8397315462489943 2.6618179571089993 7 0.5645492893217726 2.0877886706603976 8 0.38805850732369507 1.6401132128748865 9 0.29126260632140344 1.3848855134783666 >10 1.4438655084674743 13.539251094398274 >50 0.0914310028895815 3.3124045388875785 >100 0.04076375467025401 3.7152093145632796 >500 0.001506638538987032 0.5032110751028901 >1k 3.0132768992953044E-4 0.29806353157594045 >5k 1.004425633098425E-4 0.43664158261930086 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9122 0.24132722034535029 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7335 0.19405121258859293 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4422 0.11698629339696766 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.6455516371996306E-5 0.0 3 0.0 0.0 0.0 2.6455516371996306E-5 0.0 4 0.0 0.0 0.0 7.936654911598892E-5 0.0 5 0.0 0.0 0.0 7.936654911598892E-5 0.0 6 0.0 0.0 0.0 7.936654911598892E-5 0.0 7 0.0 0.0 0.0 1.0582206548798522E-4 0.0 8 0.0 0.0 0.0 1.0582206548798522E-4 0.0 9 0.0 0.0 0.0 1.0582206548798522E-4 0.0 10-11 0.0 0.0 0.0 1.0582206548798522E-4 0.0 12-13 0.0 0.0 0.0 1.0582206548798522E-4 0.0 14-15 0.0 0.0 0.0 1.3227758185998154E-4 0.0 16-17 0.0 0.0 0.0 1.8518861460397414E-4 0.0 18-19 0.0 0.0 0.0 3.042384382779575E-4 0.0 20-21 0.0 0.0 0.0 3.306939546499539E-4 0.0 22-23 0.0 0.0 0.0 3.43921712835952E-4 0.0 24-25 0.0 0.0 0.0 5.026548110679298E-4 0.0 26-27 0.0 0.0 0.0 5.952491183699169E-4 0.0 28-29 0.0 0.0 0.0 0.00257941284626964 0.0 30-31 0.0 0.0 0.0 0.008161526800760861 0.0 32-33 0.0 0.0 0.0 0.019868092795369226 0.0 34-35 0.0 0.0 0.0 0.03683930654800485 0.0 36-37 0.0 0.0 0.0 0.06500120372599492 0.0 38-39 0.0 0.0 0.0 0.10505485551319733 0.0 40-41 0.0 0.0 0.0 0.1498705002473591 0.0 42-43 0.0 0.0 0.0 0.19803276780257836 0.0 44-45 0.0 0.0 0.0 0.25823229530705594 0.0 46-47 0.0 0.0 0.0 0.3307733211990698 0.0 48-49 0.0 0.0 0.0 0.41168751802282055 0.0 50-51 0.0 2.6455516371996306E-5 0.0 0.49937432703780227 0.0 52-53 0.0 2.6455516371996306E-5 0.0 0.5889923887479398 0.0 54-55 0.0 2.6455516371996306E-5 0.0 0.6903831552436157 0.0 56-57 0.0 2.6455516371996306E-5 0.0 0.8112584095472668 0.0 58-59 0.0 5.291103274399261E-5 0.0 0.9506525253113153 0.0 60-61 0.0 5.291103274399261E-5 0.0 1.1012240967425324 0.0 62-63 0.0 5.291103274399261E-5 0.0 1.2569809493826605 0.0 64-65 0.0 5.291103274399261E-5 0.0 1.4234126028788894 0.0 66-67 0.0 5.291103274399261E-5 0.0 1.6163791392962303 0.0 68-69 0.0 5.291103274399261E-5 0.0 1.842282793596707 0.0 70-71 0.0 5.291103274399261E-5 0.0 2.0900254766622663 0.0 72-73 0.0 6.613879092999077E-5 0.0 2.3472524623471864 0.0 74-75 0.0 7.936654911598892E-5 0.0 2.6111197826414774 0.0 76-77 0.0 7.936654911598892E-5 0.0 2.902051096184321 0.0 78-79 0.0 7.936654911598892E-5 0.0 3.2340149156201305 0.0 80-81 0.0 7.936654911598892E-5 0.0 3.596958144727548 0.0 82-83 0.0 7.936654911598892E-5 0.0 3.9774942922223424 0.0 84-85 0.0 7.936654911598892E-5 0.0 4.369538589338956 0.0 86-87 0.0 7.936654911598892E-5 0.0 4.784903424137484 0.0 88-89 0.0 7.936654911598892E-5 0.0 5.240348366239587 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8830 0.0 38.393936 1 GTATCAA 13405 0.0 32.506187 1 ATCAACG 15845 0.0 27.002201 3 TATCAAC 16410 0.0 26.245558 2 CAACGCA 16370 0.0 26.136217 5 TCAACGC 16445 0.0 26.10336 4 AACGCAG 16775 0.0 25.502846 6 GTACATG 12630 0.0 23.313396 1 ACGCAGA 18890 0.0 22.346815 7 TACATGG 12755 0.0 22.300623 2 CGCAGAG 18955 0.0 22.070744 8 GAGTACT 9290 0.0 21.523691 12-13 ACATGGG 12960 0.0 20.888824 3 GTACTTT 9995 0.0 19.697996 14-15 GCAGAGT 20225 0.0 19.515402 9 CATGGGG 7700 0.0 18.193823 4 AGTACTT 9825 0.0 18.08769 12-13 CAGAGTA 19020 0.0 17.87789 10-11 AGAGTAC 17085 0.0 17.53431 10-11 AGTACAT 9750 0.0 17.426607 2 >>END_MODULE