FastQCFastQC Report
Fri 27 May 2016
SRR938990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3779930
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91730.24267645168032215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81980.2168823232176257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47400.12539914760326248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92750.035.9556541
GTATCAA140500.031.602591
ATCAACG166700.026.387983
TATCAAC169250.026.046482
CAACGCA170850.025.830335
TCAACGC171650.025.7099444
AACGCAG175450.025.1531056
GTACATG122550.022.8973431
TACATGG124850.022.044562
ACGCAGA199800.021.6601437
CGCAGAG201650.021.3908318
GAGTACT101250.020.64654412-13
ACATGGG126550.020.4735183
GCAGAGT215800.019.1748859
GTACTTT109550.018.90898914-15
ACCGTAT2603.3194738E-718.2510818
AGTACTT108800.018.0143112-13
CATGGGG72050.017.7824574
AGAGTAC181950.017.27946510-11
CAGAGTA203450.016.96960810-11
Produced by FastQC (version 0.11.5)