##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938990_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3779930 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16148923392761 33.0 31.0 34.0 30.0 34.0 2 32.396450727923536 34.0 31.0 34.0 31.0 34.0 3 32.49531128883339 34.0 31.0 34.0 30.0 34.0 4 36.0688827570881 37.0 35.0 37.0 35.0 37.0 5 35.97458418542142 37.0 35.0 37.0 35.0 37.0 6 36.01337881918448 37.0 35.0 37.0 35.0 37.0 7 35.951180048307776 37.0 35.0 37.0 35.0 37.0 8 35.91552727166905 37.0 35.0 37.0 35.0 37.0 9 37.60116933382364 39.0 37.0 39.0 35.0 39.0 10-11 37.52069019796663 39.0 37.0 39.0 34.5 39.0 12-13 37.44547795858654 39.0 37.0 39.0 34.0 39.0 14-15 38.725827065580575 40.0 38.0 41.0 34.0 41.0 16-17 38.708711007875806 40.0 38.0 41.0 34.0 41.0 18-19 38.79348929742085 40.0 38.0 41.0 34.0 41.0 20-21 38.785630686282545 40.0 38.0 41.0 34.0 41.0 22-23 38.72504623101486 40.0 38.0 41.0 34.0 41.0 24-25 38.67295862621795 40.0 38.0 41.0 34.0 41.0 26-27 38.52141931199784 40.0 38.0 41.0 34.0 41.0 28-29 38.338932731558515 40.0 38.0 41.0 34.0 41.0 30-31 38.184853026378796 40.0 38.0 41.0 33.0 41.0 32-33 38.05203059315913 40.0 37.5 41.0 33.0 41.0 34-35 38.0454358943155 40.0 38.0 41.0 33.0 41.0 36-37 37.953793324215 40.0 37.0 41.0 33.0 41.0 38-39 37.83714526459485 40.0 37.0 41.0 33.0 41.0 40-41 37.681955618225736 40.0 37.0 41.0 33.0 41.0 42-43 37.52329884415849 40.0 37.0 41.0 32.0 41.0 44-45 37.29342117446619 40.0 37.0 41.0 31.5 41.0 46-47 37.019133555383306 40.0 36.0 41.0 31.0 41.0 48-49 36.75967412094933 39.5 36.0 41.0 30.5 41.0 50-51 36.498157505562276 39.0 36.0 41.0 30.0 41.0 52-53 36.25935149592718 39.0 35.0 40.5 30.0 41.0 54-55 35.99814771702121 39.0 35.0 40.0 29.0 41.0 56-57 35.7159075697169 38.0 35.0 40.0 28.5 41.0 58-59 35.39784572730183 38.0 34.5 40.0 28.0 41.0 60-61 34.98656813750519 38.0 34.0 40.0 27.5 41.0 62-63 34.653134052747006 37.0 34.0 40.0 27.0 41.0 64-65 34.38121393782424 37.0 34.0 39.5 26.0 41.0 66-67 33.92332291867839 36.0 33.0 39.0 26.0 40.5 68-69 33.50264078964425 36.0 33.0 39.0 26.0 40.0 70-71 33.04354617678105 35.0 32.0 38.0 25.0 40.0 72-73 32.521507408867365 35.0 32.0 37.5 23.5 39.5 74-75 32.047587521462034 35.0 31.0 37.0 22.5 39.0 76-77 30.076203130745807 33.0 29.0 35.0 20.0 37.0 78-79 31.06232178902784 34.0 30.5 36.0 20.0 38.0 80-81 31.070758320921286 34.0 31.0 35.5 20.0 37.0 82-83 30.750819591897205 34.0 31.0 35.0 20.0 37.0 84-85 30.348359890262515 34.0 30.5 35.0 18.0 36.0 86-87 29.75774405875241 34.0 30.0 35.0 12.5 36.0 88-89 29.187943559801372 34.0 29.0 35.0 7.0 35.5 90-91 28.72556740468739 34.0 29.0 35.0 2.0 35.0 92-93 28.352243427788345 33.0 29.0 35.0 2.0 35.0 94-95 27.93537472916165 33.0 28.0 35.0 2.0 35.0 96-97 27.289649808329784 33.0 27.0 35.0 2.0 35.0 98-99 26.347008542486236 33.0 25.0 34.0 2.0 35.0 100-101 21.92668977996947 28.5 8.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15314613758643958 1101 2 0.11781688466341222 1101 3 0.11392495400713898 1101 4 0.08510212826316632 1101 5 0.16466428664737265 1101 6 0.11304518190077317 1101 7 0.10148750427001119 1101 8 0.18976075230892064 1101 9 0.20363123559359053 1101 10-11 0.21047168905789704 1101 12-13 0.1877861977112545 1101 14-15 0.20683246371444852 1101 16-17 0.189159199944136 1101 18-19 0.27748799937402424 1101 20-21 0.2779845264474545 1101 22-23 0.27761701671509087 1101 24-25 0.3018975733471123 1101 26-27 0.24227251102607283 1101 28-29 0.32461958579180816 1101 30-31 0.16326468852408738 1101 32-33 0.20054578190415384 1101 34-35 0.27455622753102915 1101 36-37 0.26844118326071253 1101 38-39 0.3447771034082976 1101 40-41 0.35988013248999806 1101 42-43 0.30148863689270655 1101 44-45 0.36280425983941456 1101 46-47 0.26605083992380685 1101 48-49 0.15549897740184093 1101 50-51 0.2574440480923812 1101 52-53 0.23761352435717242 1101 54-55 0.2971278377226696 1101 56-57 0.09907808271925234 1101 58-59 0.26534354505562163 1101 60-61 0.3413326721668284 1101 62-63 0.28882534586720254 1101 64-65 0.2759947428409859 1101 66-67 0.2247350185568351 1101 68-69 0.17951655060050342 1101 70-71 0.14044248938571258 1101 72-73 7.246952511437144E-4 1101 74-75 0.06904781718275643 1101 76-77 -0.024297261285926908 1101 78-79 0.21266744627947531 1101 80-81 0.20143230936989553 1101 82-83 0.18079480534482073 1101 84-85 0.2610388960938117 1101 86-87 0.4627974931195489 1101 88-89 0.5476489271912115 1101 90-91 0.3718322256463864 1101 92-93 0.5422799617128717 1101 94-95 0.6313027630586951 1101 96-97 0.5472345514351815 1101 98-99 0.3670144392235457 1101 100-101 0.7853090722145488 1102 1 0.1011038360087042 1102 2 0.09481931720300452 1102 3 0.12442701178400029 1102 4 0.09630709924547176 1102 5 0.1113935960771002 1102 6 0.1437536637201191 1102 7 0.16468288182328195 1102 8 0.09533328164047816 1102 9 0.15121168528402507 1102 10-11 0.19485175814351408 1102 12-13 0.15556110762525321 1102 14-15 0.19459440919921178 1102 16-17 0.21417601144034393 1102 18-19 0.2254001731346662 1102 20-21 0.225313269817633 1102 22-23 0.17302138757980146 1102 24-25 0.16982229513394032 1102 26-27 0.22114953415446337 1102 28-29 0.18368937245672612 1102 30-31 0.2073019522464321 1102 32-33 0.1595647301073484 1102 34-35 0.20757282523224063 1102 36-37 0.2704183208688633 1102 38-39 0.24031261015837657 1102 40-41 0.28275716059037137 1102 42-43 0.16122917428880612 1102 44-45 0.23760786675990886 1102 46-47 0.21339387772698615 1102 48-49 0.1403530933906012 1102 50-51 0.24052907262287704 1102 52-53 0.2688526093145853 1102 54-55 0.1741977337710665 1102 56-57 0.2427130513771587 1102 58-59 0.17396715896174442 1102 60-61 0.10420325534240504 1102 62-63 0.16903100546827687 1102 64-65 0.21519399356409963 1102 66-67 0.22497367343825658 1102 68-69 0.38534830636707085 1102 70-71 0.34364640009398784 1102 72-73 0.31318406487907424 1102 74-75 0.3338212690645861 1102 76-77 0.0723285999536607 1102 78-79 0.12456759282849106 1102 80-81 0.291691399075539 1102 82-83 0.28693640848412727 1102 84-85 0.28381303535142166 1102 86-87 0.4295234713248881 1102 88-89 0.4514870135271032 1102 90-91 0.48363302588603574 1102 92-93 0.5503339424638618 1102 94-95 0.5169539801364387 1102 96-97 0.24107156879608382 1102 98-99 0.3906693952277678 1102 100-101 0.9362043698432885 1103 1 -0.014007834069403202 1103 2 0.025641938932650987 1103 3 0.06527670021644383 1103 4 0.06618972026380021 1103 5 0.05889130577045876 1103 6 0.04325825843702091 1103 7 0.08384670834619357 1103 8 0.05134314436924825 1103 9 0.007939667986846644 1103 10-11 0.10393624255777922 1103 12-13 0.051121132055186536 1103 14-15 0.1285121641958824 1103 16-17 0.08820163466742059 1103 18-19 0.15471692591522412 1103 20-21 0.1700160082517428 1103 22-23 0.13504644788815057 1103 24-25 0.1371029499838059 1103 26-27 0.16179962558276628 1103 28-29 0.20838574586193914 1103 30-31 0.16926032691974768 1103 32-33 0.11102755232926853 1103 34-35 0.053530234726800074 1103 36-37 5.890790186171557E-4 1103 38-39 0.06071194520294654 1103 40-41 0.018728495885135032 1103 42-43 0.024148036726920452 1103 44-45 0.08128937984623974 1103 46-47 0.14521555757952598 1103 48-49 0.03829387164108766 1103 50-51 -0.02507000046212937 1103 52-53 0.07026125520680893 1103 54-55 0.12226848002471513 1103 56-57 0.04531406708368735 1103 58-59 -0.058462939162716054 1103 60-61 -0.04392408938583969 1103 62-63 0.07796096316965873 1103 64-65 0.0888003404422264 1103 66-67 0.11464589284656768 1103 68-69 0.14040491710569114 1103 70-71 0.06600819373214506 1103 72-73 0.14081411566440494 1103 74-75 0.11547686336083274 1103 76-77 0.022542331246423686 1103 78-79 0.22226818015263916 1103 80-81 0.287743762782366 1103 82-83 0.2560309126066791 1103 84-85 0.12579828050314745 1103 86-87 -0.0010042006075430265 1103 88-89 0.33941053629465046 1103 90-91 0.4427910637892296 1103 92-93 0.33694956830317935 1103 94-95 0.1556035331740553 1103 96-97 0.06151275482539731 1103 98-99 0.13814683862009502 1103 100-101 0.5049333447057158 1104 1 0.007507243272570463 1104 2 0.03546312707615584 1104 3 0.050920346619363954 1104 4 -0.035759598251310365 1104 5 0.04025612282444513 1104 6 -0.01777945015868454 1104 7 -0.02132768138700669 1104 8 0.011864244192821616 1104 9 0.0024029766173541134 1104 10-11 0.057917172131901395 1104 12-13 0.0671918434457055 1104 14-15 0.042474601115138455 1104 16-17 0.05723329229123664 1104 18-19 0.021840813376705626 1104 20-21 0.05880048802347204 1104 22-23 -0.0411786119950861 1104 24-25 0.02892168489811553 1104 26-27 0.02717978420326972 1104 28-29 0.04208388777365002 1104 30-31 0.015265435567350494 1104 32-33 -0.0012026284554593758 1104 34-35 -0.04273002292966055 1104 36-37 -0.1517155793903484 1104 38-39 0.06505985586839103 1104 40-41 0.053204706332245166 1104 42-43 -0.06657625713489068 1104 44-45 -0.07034479919966685 1104 46-47 -0.01471986747275622 1104 48-49 -0.027717730037572608 1104 50-51 -0.07186091291450225 1104 52-53 -0.07779428954149381 1104 54-55 0.028208446982091573 1104 56-57 0.02351155938595184 1104 58-59 -0.015168445050868229 1104 60-61 0.05234080177355338 1104 62-63 0.08722643730796875 1104 64-65 -0.011142288366848163 1104 66-67 0.11770480894692525 1104 68-69 0.1013524140797486 1104 70-71 7.15397703388021E-4 1104 72-73 0.09312181457034541 1104 74-75 0.11936157690619353 1104 76-77 0.010083301368677411 1104 78-79 0.24928793557108264 1104 80-81 0.25557090110950753 1104 82-83 0.3276354969715065 1104 84-85 0.1828780585483969 1104 86-87 0.04887362042987675 1104 88-89 0.2911969852668399 1104 90-91 0.37381137798514885 1104 92-93 0.13024900226527336 1104 94-95 0.3339443806961171 1104 96-97 0.3307944454581211 1104 98-99 0.4536316761135737 1104 100-101 0.9087244385156943 1105 1 -0.13329124902627854 1105 2 -0.027798214406068666 1105 3 -0.03278741659300266 1105 4 3.797352776118146E-4 1105 5 -0.00851467186220134 1105 6 8.101329248262346E-4 1105 7 -0.0030093850437680203 1105 8 -0.02847486573051583 1105 9 -0.042687021747589426 1105 10-11 -0.08733159863282225 1105 12-13 -0.01850027516517372 1105 14-15 0.029513805943892635 1105 16-17 -0.024115317386694812 1105 18-19 -0.028754276033694737 1105 20-21 -0.02224249339833051 1105 22-23 -0.11623160166559643 1105 24-25 -0.021402042872303184 1105 26-27 0.009935324965304915 1105 28-29 -0.01913051960450929 1105 30-31 -0.11605449875758467 1105 32-33 -0.1412754174891262 1105 34-35 -0.05034870881878106 1105 36-37 -0.03555043991623563 1105 38-39 -0.05764721130098849 1105 40-41 -0.09134701030538395 1105 42-43 -0.17676507405431607 1105 44-45 -0.21095802707221623 1105 46-47 -0.059588574652131854 1105 48-49 -0.11764643824906074 1105 50-51 0.012932858605587683 1105 52-53 0.12244071578050608 1105 54-55 -0.021638356901334532 1105 56-57 -0.038821737271497625 1105 58-59 -0.10890089745378617 1105 60-61 -0.07205600279520752 1105 62-63 -0.08983826862417033 1105 64-65 -0.055363974423677575 1105 66-67 -0.006208368211034099 1105 68-69 -0.045688332184987246 1105 70-71 0.059497998830913446 1105 72-73 -0.1482206593235489 1105 74-75 0.033807311206821566 1105 76-77 -0.13555112798954738 1105 78-79 0.003963718331071675 1105 80-81 -0.01571707500827202 1105 82-83 0.06987912669521634 1105 84-85 0.03154668254415327 1105 86-87 -0.07590131531178912 1105 88-89 0.08654806528691594 1105 90-91 0.22800273190746623 1105 92-93 0.33910264202029694 1105 94-95 0.26086795685455044 1105 96-97 0.18327123272804968 1105 98-99 0.12117307149662082 1105 100-101 0.8195601941679804 1106 1 -0.04562077901194783 1106 2 -0.12706158884339658 1106 3 -0.0980735353490374 1106 4 -0.06005407176395039 1106 5 -0.018664209525134368 1106 6 -0.0543271766998501 1106 7 -0.09680986545205883 1106 8 -0.077627507010817 1106 9 -0.15105317967822884 1106 10-11 -0.007755223717069271 1106 12-13 -0.027778816901403047 1106 14-15 0.011994406422928705 1106 16-17 -0.0443467485556468 1106 18-19 -0.03627709309628813 1106 20-21 -0.0398292092369914 1106 22-23 0.0432043648164111 1106 24-25 0.03350993193566154 1106 26-27 -0.028822518496717464 1106 28-29 0.09758355382608386 1106 30-31 0.08328354288105544 1106 32-33 0.05813966636375767 1106 34-35 0.03713685988133619 1106 36-37 -0.0668135876345346 1106 38-39 0.07543281403150814 1106 40-41 0.07188824834991436 1106 42-43 0.11002283823718528 1106 44-45 0.057413772606949465 1106 46-47 0.06878222256759159 1106 48-49 -0.05848981991487534 1106 50-51 0.06638460114747602 1106 52-53 0.14532698886866768 1106 54-55 0.06221045502029199 1106 56-57 0.11514230138993042 1106 58-59 0.17428908879226412 1106 60-61 0.0953792768272308 1106 62-63 -0.0036541123063500436 1106 64-65 0.0027786736618438113 1106 66-67 0.10783902466222628 1106 68-69 -0.15566909822282327 1106 70-71 -0.021177183835632718 1106 72-73 0.09148273070143631 1106 74-75 0.12122818630292187 1106 76-77 -0.11999493893531366 1106 78-79 -0.009350232662505675 1106 80-81 0.1472034580058299 1106 82-83 0.22217446377985794 1106 84-85 0.2423392804593405 1106 86-87 0.23694744641401755 1106 88-89 0.29893993571961275 1106 90-91 0.49818322050591135 1106 92-93 0.44050414735595567 1106 94-95 0.6075580666959866 1106 96-97 0.4208014859124418 1106 98-99 0.4192151938810369 1106 100-101 0.6831138065517344 1107 1 -0.11157609335344887 1107 2 -0.07750502664652004 1107 3 -0.07190522688313905 1107 4 -0.057930167882197736 1107 5 -0.1583617448472836 1107 6 -0.1236345894334363 1107 7 -0.060573215766488886 1107 8 -0.0702820018198338 1107 9 -0.10592941805450806 1107 10-11 -0.04070534707429374 1107 12-13 -0.05088341499089921 1107 14-15 -0.009097462727211791 1107 16-17 -0.09147829163660504 1107 18-19 -0.06768474927466883 1107 20-21 -0.05664197242152369 1107 22-23 0.03801947525855098 1107 24-25 -0.08793042638250625 1107 26-27 -0.0695222631205965 1107 28-29 -0.0770447110102026 1107 30-31 -0.06752281066113852 1107 32-33 -0.08638442457583295 1107 34-35 -0.1796292894173206 1107 36-37 -0.005586739699772636 1107 38-39 -0.09717107018650495 1107 40-41 -0.16804011210900427 1107 42-43 -0.09099640761166228 1107 44-45 -0.08461929693714865 1107 46-47 -0.29453340212587875 1107 48-49 -0.20655422867498885 1107 50-51 -0.21888456482462715 1107 52-53 -0.1406217030170538 1107 54-55 -0.17059231224268956 1107 56-57 -0.10622758757423156 1107 58-59 -0.10950436515829409 1107 60-61 -0.24369623092783854 1107 62-63 -0.25379178137401226 1107 64-65 -0.21501838033483267 1107 66-67 -0.02188309606092531 1107 68-69 -0.06441268298767966 1107 70-71 -0.040205020807917435 1107 72-73 -0.022511093406826888 1107 74-75 -0.15742956610040437 1107 76-77 -0.348430621094149 1107 78-79 0.0034338460245457725 1107 80-81 0.008493590690129338 1107 82-83 0.053403766812994746 1107 84-85 -0.07802219692478474 1107 86-87 -0.1151836779235218 1107 88-89 -0.01625156625565083 1107 90-91 0.2375284951012091 1107 92-93 0.2741500444448839 1107 94-95 0.24814166738456223 1107 96-97 0.18344725020808283 1107 98-99 0.20468907088864086 1107 100-101 0.8182796633609932 1108 1 0.007444314631925408 1108 2 0.022383984594725348 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-3.8691738746500555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 23.0 5 81.0 6 169.0 7 448.0 8 1033.0 9 1854.0 10 3111.0 11 4817.0 12 6909.0 13 9434.0 14 11513.0 15 15045.0 16 19853.0 17 26846.0 18 26595.0 19 20056.0 20 19162.0 21 20750.0 22 22353.0 23 25259.0 24 29578.0 25 35332.0 26 42804.0 27 52241.0 28 63412.0 29 77749.0 30 94126.0 31 115159.0 32 142715.0 33 181700.0 34 245263.0 35 350060.0 36 556158.0 37 870797.0 38 641439.0 39 46075.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.18840311777506 19.487103478172173 14.551113278593771 23.773380125458996 2 15.84172193664962 20.314053434851967 39.6018973896342 24.24232723886421 3 19.39427449714677 23.89419909892511 29.61755905532642 27.093967348601694 4 13.621257536515227 16.628958737331114 35.88873338924265 33.861050336911006 5 14.23160125918042 36.882509916217785 34.031099608219115 14.854789216382681 6 33.07106216252682 35.41200498421929 16.890286328053694 14.6266465252002 7 29.16114848687674 32.14334656991002 20.417520959382845 18.27798398383039 8 26.089372025407876 35.94222644334684 19.290277862288455 18.678123668956832 9 26.235644575428648 16.938408912334353 20.16920948271529 36.656737029521715 10-11 25.332003502710364 26.395528488622805 26.77648792437955 21.495980084287275 12-13 25.88110097276934 23.552750976869945 27.535615738915798 23.030532311444922 14-15 22.865092084885188 24.98444395883258 25.630853026628873 26.51961092965336 16-17 22.692643991073684 28.192275588749244 26.31892661981277 22.7961538003643 18-19 22.629757693925548 26.942006862560948 28.28369837536674 22.144537068146764 20-21 23.20331328887043 26.266346202178347 28.30028598413198 22.23005452481924 22-23 23.008283222176072 26.099372210596496 28.08906514141796 22.80327942580947 24-25 22.801043934676038 26.24811835139804 28.243657951337724 22.707179762588193 26-27 22.737074496088553 26.463320749326048 28.155825636982694 22.64377911760271 28-29 22.61861992153294 26.49875791350633 28.29728328302376 22.585338881936966 30-31 22.77109629014294 26.32161177587945 28.156791263330273 22.750500670647337 32-33 22.6281968184596 26.46168050731098 28.12579862590048 22.784324048328937 34-35 22.96223463486143 26.415476955499955 27.99547811427947 22.62681029535914 36-37 23.00606578211258 26.286744692523335 28.024090625454324 22.68309889990977 38-39 22.812091869889446 26.46941392251534 28.105374035040185 22.61312017255503 40-41 23.00553528354687 26.315395416607966 28.01698748029845 22.662081819546714 42-43 22.82020919049796 26.386768023738167 27.8627446622983 22.93027812346557 44-45 22.84289575670577 26.56355946440094 27.76537156768257 22.82817321121072 46-47 22.910052210168747 26.672808780091618 27.447098678144876 22.970040331594756 48-49 23.049643279251463 26.659312008793844 27.379274812214817 22.911769899739877 50-51 22.94962135958161 26.837879941707538 27.281819611627856 22.930679087082996 52-53 23.114674081266052 26.85988893974227 27.111507355956327 22.913929623035347 54-55 22.97846785522483 26.73163259637083 27.183016616709832 23.106882931694503 56-57 22.89103237361538 27.01251081369232 27.114576195855477 22.98188061683682 58-59 22.996641596922053 26.76596590696394 27.163222834124994 23.074169661989014 60-61 23.031338107258005 26.693425301977147 27.321613433799698 22.95362315696515 62-63 22.926667954168465 26.67452836428188 27.40698901831515 22.991814663234507 64-65 22.838623585900613 26.572654182022465 27.407538803364673 23.18118342871225 66-67 22.783849340870276 26.73864091375617 27.206527313568017 23.270982431805535 68-69 22.632185694585452 26.76922771661668 27.37165703778907 23.226929551008798 70-71 22.74105905943484 26.60037707052473 27.256130041578817 23.402433828461618 72-73 22.809483244398706 26.601577277886097 27.099086491019676 23.48985298669552 74-75 22.70897873770149 26.81580082170834 26.942469834097455 23.532750606492712 76-77 22.783641623970848 26.637979843732307 27.110279378664075 23.46809915363277 78-79 22.708645618988307 26.628140339255317 27.166669400406253 23.496544641350123 80-81 22.615511398359228 26.827705804075734 27.06478691404338 23.491995883521653 82-83 22.688872545258775 26.77930543687317 27.026227998931198 23.50559401893686 84-85 22.72174352435098 26.545359305595607 26.95231128618784 23.780585883865573 86-87 22.534573347000965 26.509862448458243 27.273036776762833 23.682527427777956 88-89 22.656006594166094 26.401044395164757 27.173765018793876 23.769183991875273 90-91 22.50696425414391 26.505404431107905 27.204245500643204 23.783385814104985 92-93 22.438709182445177 26.423121592198797 27.10597815303458 24.032191072321446 94-95 22.45976777347729 26.37171852388801 27.063649326839386 24.10486437579532 96-97 22.411378795462745 26.222754627890417 27.154152958220145 24.211713618426693 98-99 22.224631143962984 26.24039334061742 27.097697576410145 24.43727793900945 100-101 22.385854411488285 25.807262348507066 27.578265204254958 24.228618035749694 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 449.0 1 317.0 2 226.0 3 305.0 4 297.5 5 302.5 6 558.5 7 1133.0 8 1875.0 9 2354.0 10 2351.5 11 2318.5 12 2510.5 13 2818.0 14 3074.5 15 3325.0 16 3655.5 17 3905.0 18 4170.0 19 4567.5 20 5181.0 21 6070.0 22 7495.0 23 9546.5 24 11739.0 25 14483.0 26 18452.0 27 23083.0 28 27441.5 29 32200.5 30 37555.0 31 44341.5 32 50833.0 33 56508.0 34 63492.0 35 70651.0 36 78989.5 37 87560.0 38 93155.0 39 97210.0 40 101689.5 41 107282.5 42 113284.5 43 120271.0 44 128076.0 45 133711.5 46 137097.0 47 141436.5 48 150606.0 49 153511.5 50 149508.0 51 147795.0 52 144856.0 53 143425.0 54 142220.0 55 133679.5 56 121669.5 57 110274.0 58 100162.5 59 89417.0 60 75744.5 61 62056.0 62 49866.5 63 39457.5 64 30253.0 65 22107.0 66 15486.0 67 11735.5 68 8900.5 69 5973.5 70 4061.0 71 2703.5 72 1817.0 73 1265.0 74 909.0 75 630.5 76 351.0 77 155.5 78 84.5 79 46.5 80 27.5 81 20.0 82 9.5 83 4.5 84 2.5 85 1.0 86 1.5 87 3.0 88 2.5 89 3.0 90 2.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4019651157561119 2 0.0 3 0.0 4 0.0 5 2.910106800919594E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012698647858558228 16-17 0.002513274055339649 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002407451989851664 36-37 0.007553049924204945 38-39 0.013174847153254161 40-41 0.0023148576825496765 42-43 6.87843425671904E-4 44-45 3.43921712835952E-4 46-47 3.968327455799446E-4 48-49 3.3069395464995383E-4 50-51 6.481601511139095E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.248718891619686E-4 60-61 0.002407451989851664 62-63 0.0 64-65 1.71960856417976E-4 66-67 0.0015741032241337802 68-69 0.0017857473551097507 70-71 1.3227758185998155E-5 72-73 0.0 74-75 0.0 76-77 0.12374567783001274 78-79 1.0582206548798524E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14717203757741545 88-89 0.4392541660824407 90-91 0.33958300815094455 92-93 0.0 94-95 0.0 96-97 5.820213601839188E-4 98-99 0.0 100-101 2.5132740553396494E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3779930.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.7660232913997 #Duplication Level Percentage of deduplicated Percentage of total 1 71.21713014469232 36.15410487672094 2 15.270528366107023 15.504479974115378 3 5.440378965501318 8.28559215830043 4 2.625367350593984 5.331178402749382 5 1.474387594936304 3.7424397492543595 6 0.9177523171269545 2.795438130420181 7 0.5953461228006501 2.1156348595579617 8 0.4255988877846939 1.7284770440057262 9 0.31227301683321756 1.4267573321247697 >10 1.5741450260371663 14.146739526895363 >50 0.09922858145116685 3.4595141785222814 >100 0.04598414094408896 4.036582647504865 >500 0.001357405990441008 0.42954848025824427 >1k 4.1766336052541673E-4 0.38311365565848315 >5k 1.0441584013135382E-4 0.4603989839116913 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9173 0.24267645168032215 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8198 0.2168823232176257 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4740 0.12539914760326248 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.291103274399261E-5 0.0 5 0.0 0.0 0.0 5.291103274399261E-5 0.0 6 0.0 0.0 0.0 5.291103274399261E-5 0.0 7 0.0 0.0 0.0 5.291103274399261E-5 0.0 8 0.0 0.0 0.0 5.291103274399261E-5 0.0 9 0.0 0.0 0.0 5.291103274399261E-5 0.0 10-11 0.0 0.0 0.0 1.0582206548798522E-4 0.0 12-13 0.0 1.3227758185998153E-5 0.0 1.0582206548798522E-4 0.0 14-15 0.0 2.6455516371996306E-5 0.0 1.0582206548798522E-4 0.0 16-17 0.0 2.6455516371996306E-5 0.0 1.8518861460397414E-4 0.0 18-19 0.0 2.6455516371996306E-5 0.0 2.7778292190596126E-4 0.0 20-21 0.0 3.9683274557994456E-5 0.0 3.174661964639557E-4 0.0 22-23 0.0 5.291103274399261E-5 0.0 3.7037722920794827E-4 0.0 24-25 0.0 5.291103274399261E-5 0.0 5.291103274399261E-4 0.0 26-27 0.0 5.291103274399261E-5 0.0 6.08476876555915E-4 0.0 28-29 0.0 5.291103274399261E-5 0.0 0.00257941284626964 0.0 30-31 0.0 7.936654911598892E-5 0.0 0.008135071284388864 0.0 32-33 0.0 7.936654911598892E-5 0.0 0.01984163727899723 0.0 34-35 0.0 7.936654911598892E-5 0.0 0.03706417843716683 0.0 36-37 0.0 7.936654911598892E-5 0.0 0.06562290836073684 0.0 38-39 0.0 7.936654911598892E-5 0.0 0.10611307616807719 0.0 40-41 0.0 1.0582206548798522E-4 0.0 0.15147105898786484 0.0 42-43 0.0 1.0582206548798522E-4 0.0 0.20012275359596607 0.0 44-45 0.0 1.0582206548798522E-4 0.0 0.2611027188334175 0.0 46-47 0.0 1.0582206548798522E-4 0.0 0.3342522216019873 0.0 48-49 0.0 1.0582206548798522E-4 0.0 0.41601299494964195 0.0 50-51 0.0 1.0582206548798522E-4 0.0 0.5046125192794575 0.0 52-53 0.0 1.0582206548798522E-4 0.0 0.5947993745915929 0.0 54-55 0.0 1.0582206548798522E-4 0.0 0.6976187389713566 0.0 56-57 0.0 1.0582206548798522E-4 0.0 0.8192347477334236 0.0 58-59 0.0 1.0582206548798522E-4 0.0 0.959422528988632 0.0 60-61 0.0 1.3227758185998154E-4 0.0 1.1113830150293789 0.0 62-63 0.0 1.3227758185998154E-4 0.0 1.268621376586339 0.0 64-65 0.0 1.3227758185998154E-4 0.0 1.4363890336593537 0.0 66-67 0.0 1.3227758185998154E-4 0.0 1.6299375914368786 0.0 68-69 0.0 1.3227758185998154E-4 0.0 1.8572301603468846 0.0 70-71 0.0 1.5873309823197785E-4 0.0 2.107049601447646 0.0 72-73 0.0 1.5873309823197785E-4 0.0 2.3665517615405576 0.0 74-75 0.0 1.8518861460397414E-4 0.0 2.632628117451911 0.0 76-77 0.0 1.8518861460397414E-4 0.0 2.9255965057553976 0.0 78-79 0.0 1.8518861460397414E-4 0.0 3.2599677771810587 0.0 80-81 0.0 1.8518861460397414E-4 0.0 3.624776120192702 0.0 82-83 0.0 1.8518861460397414E-4 0.0 4.007997502599254 0.0 84-85 0.0 1.8518861460397414E-4 0.0 4.401033881579817 0.0 86-87 0.0 1.8518861460397414E-4 0.0 4.817390798242296 0.0 88-89 0.0 2.1164413097597045E-4 0.0 5.274409843568532 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9275 0.0 35.955654 1 GTATCAA 14050 0.0 31.60259 1 ATCAACG 16670 0.0 26.38798 3 TATCAAC 16925 0.0 26.04648 2 CAACGCA 17085 0.0 25.83033 5 TCAACGC 17165 0.0 25.709944 4 AACGCAG 17545 0.0 25.153105 6 GTACATG 12255 0.0 22.897343 1 TACATGG 12485 0.0 22.04456 2 ACGCAGA 19980 0.0 21.660143 7 CGCAGAG 20165 0.0 21.390831 8 GAGTACT 10125 0.0 20.646544 12-13 ACATGGG 12655 0.0 20.473518 3 GCAGAGT 21580 0.0 19.174885 9 GTACTTT 10955 0.0 18.908989 14-15 ACCGTAT 260 3.3194738E-7 18.251081 8 AGTACTT 10880 0.0 18.01431 12-13 CATGGGG 7205 0.0 17.782457 4 AGAGTAC 18195 0.0 17.279465 10-11 CAGAGTA 20345 0.0 16.969608 10-11 >>END_MODULE