FastQCFastQC Report
Fri 27 May 2016
SRR938989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172890
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT270950.853953335917728No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT157060.49500613005808586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147000.4633000198557152No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT124410.3921030984370716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113290.35705618537043515No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC103790.32711502762465766No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT80540.25383798366788635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73310.23105118677294204No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT67780.21362228126408414No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC66270.2088632130329132No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC64270.2025598114022232No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC58800.18532000794228606No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG57870.1823889261840152No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA57570.1814434159394117No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA50340.15865661904446735No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA42880.13514493096199365No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA38880.12253812770061363No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA37960.11963856295049624No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC36760.11585652197208224No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT36420.11478494369486494No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG32510.10246179350686599No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT9900.028.2281763
CTTTGCG10700.026.1176572
TTGCGTT11250.025.2618274
GTACATG142100.023.2495251
GAGTACT117100.022.7218312-13
TACATGG145500.022.3319742
ACATGGG145450.021.0696133
AGTACTT121850.020.9618612-13
GTACTTT127900.020.63725514-15
CATGGGG50700.019.8058684
ACTTTTT152800.017.61675316-17
CATGGGA93250.017.3717384
GTATAGG9600.017.3059641
TACTTTT149050.016.99414814-15
GTAAGCG3000.016.64715494-95
AGAGTAC256600.016.40194310-11
TATAGCG2104.0934E-415.7883925
GTCGGGA6750.015.4377822
ATGGGAT39050.015.0404155
GTCCTAT8300.014.8694251
Produced by FastQC (version 0.11.5)