##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938989_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3172890 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.781709734658307 33.0 31.0 34.0 30.0 34.0 2 31.862841447387083 33.0 31.0 34.0 30.0 34.0 3 31.90380977594559 34.0 31.0 34.0 30.0 34.0 4 35.430897698943234 37.0 35.0 37.0 33.0 37.0 5 35.47968445171437 37.0 35.0 37.0 33.0 37.0 6 35.570609129216585 37.0 35.0 37.0 33.0 37.0 7 35.50268808562541 37.0 35.0 37.0 33.0 37.0 8 35.49353144924659 37.0 35.0 37.0 33.0 37.0 9 37.08479335873604 39.0 37.0 39.0 33.0 39.0 10-11 36.96917037779438 39.0 37.0 39.0 33.0 39.0 12-13 36.86837867054956 39.0 37.0 39.0 33.0 39.0 14-15 38.03248631373921 40.0 38.0 41.0 33.0 41.0 16-17 37.96221804096581 40.0 38.0 41.0 33.0 41.0 18-19 37.912093863953686 40.0 38.0 41.0 33.0 41.0 20-21 38.03390048189505 40.0 38.0 41.0 33.0 41.0 22-23 37.969926785990054 40.0 38.0 41.0 33.0 41.0 24-25 37.88235662125066 40.0 38.0 41.0 32.5 41.0 26-27 37.75275017413147 40.0 37.5 41.0 32.0 41.0 28-29 37.634639398151215 40.0 37.0 41.0 32.0 41.0 30-31 37.44867581290244 40.0 37.0 41.0 32.0 41.0 32-33 37.2357815745267 40.0 36.5 41.0 31.0 41.0 34-35 36.896618697780255 40.0 36.0 41.0 30.5 41.0 36-37 36.61816246387363 39.5 36.0 41.0 30.0 41.0 38-39 36.38117961858117 39.0 35.0 41.0 30.0 41.0 40-41 36.26106514880755 39.0 35.0 41.0 30.0 41.0 42-43 36.0467962961212 39.0 35.0 41.0 29.5 41.0 44-45 35.94523699214281 39.0 35.0 41.0 28.5 41.0 46-47 35.687549205928974 39.0 35.0 41.0 27.0 41.0 48-49 35.421851529677994 39.0 35.0 41.0 26.0 41.0 50-51 34.302966223222356 38.0 34.0 40.0 24.0 40.5 52-53 34.42341083365638 38.0 34.0 39.5 24.0 40.5 54-55 34.93408249261714 39.0 35.0 40.0 24.5 41.0 56-57 34.83268723466619 39.0 34.5 41.0 24.0 41.0 58-59 34.63587581038107 38.5 34.0 40.0 23.5 41.0 60-61 34.30359010239876 38.0 34.0 40.0 23.0 41.0 62-63 33.95075262615471 38.0 34.0 40.0 22.0 41.0 64-65 33.5708639442275 37.0 33.0 40.0 21.5 41.0 66-67 33.134400814399484 36.5 33.0 39.0 19.5 41.0 68-69 32.69120108166372 36.0 32.0 39.0 18.0 41.0 70-71 32.2157487338042 35.5 32.0 39.0 15.5 40.0 72-73 31.767571047215633 35.0 31.0 38.0 12.5 40.0 74-75 31.300730564248997 35.0 31.0 37.0 8.0 39.0 76-77 30.87486439807242 35.0 31.0 37.0 7.0 39.0 78-79 30.466922427187832 35.0 30.5 36.0 3.5 39.0 80-81 29.992181260617294 34.0 30.0 36.0 2.0 37.5 82-83 29.518482361506386 34.0 29.5 35.0 2.0 37.0 84-85 29.09111125819048 34.0 29.0 35.0 2.0 36.5 86-87 28.673796917006264 34.0 29.0 35.0 2.0 36.0 88-89 28.17804351868486 34.0 28.0 35.0 2.0 36.0 90-91 27.70206420644901 34.0 27.0 35.0 2.0 35.0 92-93 27.20201078512019 33.5 26.0 35.0 2.0 35.0 94-95 26.665460668349674 33.0 25.0 35.0 2.0 35.0 96-97 26.007682743492527 33.0 24.5 35.0 2.0 35.0 98-99 25.162688274727458 32.5 18.5 35.0 2.0 35.0 100-101 21.93910598854672 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16686760622358676 1101 2 0.31735387452304664 1101 3 0.2814407531518661 1101 4 0.2384751695816547 1101 5 0.10287379067623448 1101 6 0.24605965885347558 1101 7 0.23415910875213797 1101 8 0.1829505748928355 1101 9 0.3129651930220092 1101 10-11 0.24083134194389544 1101 12-13 0.2572464089233435 1101 14-15 0.3633035420002102 1101 16-17 0.3954725805405914 1101 18-19 0.4651050998083903 1101 20-21 0.4700642316335646 1101 22-23 0.4244275034935754 1101 24-25 0.367622489521203 1101 26-27 0.46393350796892463 1101 28-29 0.3686052351555844 1101 30-31 0.3404089847662348 1101 32-33 0.37711726074515894 1101 34-35 0.35606197041485643 1101 36-37 0.3744801247752392 1101 38-39 0.4616219625941511 1101 40-41 0.23082710860020228 1101 42-43 0.15455347979019507 1101 44-45 0.3123216659101189 1101 46-47 0.2931449036393019 1101 48-49 0.17467435868931602 1101 50-51 0.27603007144688974 1101 52-53 0.2795218655385554 1101 54-55 0.2685210178264228 1101 56-57 0.19225240151268252 1101 58-59 0.2906104005823096 1101 60-61 0.4752685536237422 1101 62-63 0.3199691282233914 1101 64-65 0.2247699610225382 1101 66-67 0.32031460719571925 1101 68-69 0.31650894656537787 1101 70-71 0.1360322770353477 1101 72-73 0.11939909203871935 1101 74-75 -0.05034736854197064 1101 76-77 0.03543754101471208 1101 78-79 -0.09984463392358123 1101 80-81 7.759998845564553E-4 1101 82-83 0.004679071361096021 1101 84-85 0.052174650233485664 1101 86-87 0.07610182179897507 1101 88-89 -0.20863136793584403 1101 90-91 -0.055913607347310545 1101 92-93 0.09852753424306471 1101 94-95 0.002868346869487226 1101 96-97 -0.23481108532036998 1101 98-99 -0.2877582217522985 1101 100-101 0.0026035155187500436 1102 1 0.2729098194422548 1102 2 0.3131225487064775 1102 3 0.2866779933725745 1102 4 0.13551727758636645 1102 5 0.3004438657576074 1102 6 0.2907290196873191 1102 7 0.33902223574538226 1102 8 0.2819776045304039 1102 9 0.3507928870598249 1102 10-11 0.37013929284174907 1102 12-13 0.36691039694619576 1102 14-15 0.5129617691278128 1102 16-17 0.6334492989345719 1102 18-19 0.5392240191748314 1102 20-21 0.5299401136628532 1102 22-23 0.4969352694908409 1102 24-25 0.5479683502144823 1102 26-27 0.5943769071730927 1102 28-29 0.5538983940062394 1102 30-31 0.5561795217541103 1102 32-33 0.5643349725658666 1102 34-35 0.46400752133065026 1102 36-37 0.3701230596581482 1102 38-39 0.47697837055522285 1102 40-41 0.4851971961002661 1102 42-43 0.2791278375969881 1102 44-45 0.4296667901057063 1102 46-47 0.4147375669494764 1102 48-49 0.4328440498259809 1102 50-51 0.38565870068153885 1102 52-53 0.4991113241351428 1102 54-55 0.5588649639706702 1102 56-57 0.5096801445353591 1102 58-59 0.42048840413889366 1102 60-61 0.5190629138107781 1102 62-63 0.5233380445714033 1102 64-65 0.4181247511034485 1102 66-67 0.5113239884859127 1102 68-69 0.5636413488414433 1102 70-71 0.40459848679747523 1102 72-73 0.33397605432210753 1102 74-75 0.25330735030391693 1102 76-77 0.3088315189818758 1102 78-79 0.25971370893937973 1102 80-81 0.2637541412541644 1102 82-83 0.1801944041390371 1102 84-85 -0.013766040142542124 1102 86-87 0.04832723493623092 1102 88-89 0.01716243685949692 1102 90-91 0.002517393858337158 1102 92-93 0.06015341756596726 1102 94-95 -0.047172445098379256 1102 96-97 0.10387884570411998 1102 98-99 -0.08619139622980754 1102 100-101 -0.1713975987142824 1103 1 0.23589848520042977 1103 2 0.25469124185751113 1103 3 0.3378685579775187 1103 4 0.4064734196492594 1103 5 0.16153097885073464 1103 6 0.23392424303585102 1103 7 0.19130206664427618 1103 8 0.15838157494058436 1103 9 0.41257040614645746 1103 10-11 0.35242201962302744 1103 12-13 0.36668759247698546 1103 14-15 0.4755026957756314 1103 16-17 0.425956735825352 1103 18-19 0.574210078063139 1103 20-21 0.5337555970190166 1103 22-23 0.4709557502303454 1103 24-25 0.5612913090348073 1103 26-27 0.5962959617912844 1103 28-29 0.4853636241310113 1103 30-31 0.5040018239633284 1103 32-33 0.5785771293591822 1103 34-35 0.39099685722940336 1103 36-37 0.41331137434096377 1103 38-39 0.5635411884617909 1103 40-41 0.488605989334836 1103 42-43 0.3751934676183595 1103 44-45 0.6181224097007529 1103 46-47 0.7055520353074982 1103 48-49 0.5690384919195637 1103 50-51 0.4767840014243845 1103 52-53 0.36187563097431763 1103 54-55 0.5813863506089731 1103 56-57 0.5623897520137646 1103 58-59 0.49523901569973816 1103 60-61 0.5934592835372356 1103 62-63 0.5519692248766361 1103 64-65 0.4909757674545361 1103 66-67 0.49674278892277357 1103 68-69 0.5173395863832511 1103 70-71 0.5118842505130559 1103 72-73 0.36567617908109185 1103 74-75 0.30427441957307977 1103 76-77 0.25740882320900127 1103 78-79 0.2300867596161673 1103 80-81 0.1386232940041907 1103 82-83 0.3227410189216222 1103 84-85 0.35679694126911343 1103 86-87 0.46320331938085246 1103 88-89 0.3315500210660609 1103 90-91 0.3101220500687383 1103 92-93 0.26574943679278107 1103 94-95 0.32881487519715336 1103 96-97 0.28964975780344204 1103 98-99 0.3965867970274495 1103 100-101 0.3986762491649607 1104 1 0.25349449537839774 1104 2 0.2632748559602085 1104 3 0.24876061402290972 1104 4 0.32180169327826746 1104 5 0.17264336641971312 1104 6 0.2848390395504836 1104 7 0.3021751453311978 1104 8 0.25370535502698033 1104 9 0.3821109367913067 1104 10-11 0.39716923242462343 1104 12-13 0.38355010810267487 1104 14-15 0.5219522076807479 1104 16-17 0.4878984524510912 1104 18-19 0.5098011314752782 1104 20-21 0.46291503641720766 1104 22-23 0.4740414201750127 1104 24-25 0.4551570801673961 1104 26-27 0.42393577938673843 1104 28-29 0.6126479572005223 1104 30-31 0.5130228303504438 1104 32-33 0.5399407531146707 1104 34-35 0.4899636660374824 1104 36-37 0.5607806476630017 1104 38-39 0.6343851585159399 1104 40-41 0.5827197605635845 1104 42-43 0.5406811186975489 1104 44-45 0.5705376657402255 1104 46-47 0.5711625191307448 1104 48-49 0.663759261239953 1104 50-51 0.6251710530871932 1104 52-53 0.5403033094400769 1104 54-55 0.6898006362836284 1104 56-57 0.6749006969336548 1104 58-59 0.5988726713168546 1104 60-61 0.614249314899574 1104 62-63 0.7007989274706858 1104 64-65 0.5587221633744761 1104 66-67 0.6239811810666538 1104 68-69 0.6103063947290366 1104 70-71 0.6420080795106387 1104 72-73 0.715373246351092 1104 74-75 0.5935696291422907 1104 76-77 0.5765488183271295 1104 78-79 0.5353737453551801 1104 80-81 0.6592225693973326 1104 82-83 0.684172612132123 1104 84-85 0.6883172357163545 1104 86-87 0.6021555496018678 1104 88-89 0.5877262150909424 1104 90-91 0.6924917257755006 1104 92-93 0.771466972796599 1104 94-95 0.7144039407252087 1104 96-97 0.6675844294604651 1104 98-99 0.8375992841755497 1104 100-101 1.003617632011089 1105 1 0.215837172723937 1105 2 0.30872322552542997 1105 3 0.24709799960642798 1105 4 0.13432672188238826 1105 5 0.1643732005158185 1105 6 0.2727537992758897 1105 7 0.23126553434721586 1105 8 0.3142996055990608 1105 9 0.28494274457162305 1105 10-11 0.35435065404933397 1105 12-13 0.351915740253709 1105 14-15 0.4096792528752502 1105 16-17 0.40546316927031256 1105 18-19 0.445452590285349 1105 20-21 0.49849140941492465 1105 22-23 0.3636205820743328 1105 24-25 0.37800998348547665 1105 26-27 0.3571666146270118 1105 28-29 0.3269249880929763 1105 30-31 0.2979717004877429 1105 32-33 0.46451169132977554 1105 34-35 0.4302525676393145 1105 36-37 0.30687082214813444 1105 38-39 0.39851023002623975 1105 40-41 0.4601958046139316 1105 42-43 0.31169084409800973 1105 44-45 0.39742911310865026 1105 46-47 0.4612154367508836 1105 48-49 0.5462195730248496 1105 50-51 0.4396787046890154 1105 52-53 0.49262560635083474 1105 54-55 0.4856190716383182 1105 56-57 0.31590648926775344 1105 58-59 0.34368281147703783 1105 60-61 0.4764060654716573 1105 62-63 0.5006612701480293 1105 64-65 0.5402510535346536 1105 66-67 0.581648872714247 1105 68-69 0.5295516035391046 1105 70-71 0.4490250274511425 1105 72-73 0.41977649291859365 1105 74-75 0.39606355076700694 1105 76-77 0.2976300540250669 1105 78-79 0.3468797522201683 1105 80-81 0.44395690864218196 1105 82-83 0.5928736275783457 1105 84-85 0.6031502591717306 1105 86-87 0.5384738419359643 1105 88-89 0.4879943787065031 1105 90-91 0.6941239483155464 1105 92-93 0.7006556884716524 1105 94-95 0.7006632370638819 1105 96-97 0.4723541663025692 1105 98-99 0.4667129140382471 1105 100-101 0.6447414163360321 1106 1 0.3165201575716452 1106 2 0.2275158253306877 1106 3 0.14933251420353955 1106 4 0.22589270780630244 1106 5 0.2552350618390449 1106 6 0.24637661910540487 1106 7 0.15886252097151754 1106 8 0.24159583119225658 1106 9 0.3674863266280326 1106 10-11 0.18880519503910165 1106 12-13 0.3335711323854511 1106 14-15 0.32598147233578345 1106 16-17 0.3175900206001998 1106 18-19 0.3446072670820115 1106 20-21 0.28099644879038266 1106 22-23 0.33168007019336443 1106 24-25 0.392404447005525 1106 26-27 0.3501856382693731 1106 28-29 0.31755842264088585 1106 30-31 0.32358726233540125 1106 32-33 0.2930945057782637 1106 34-35 0.2981293783979524 1106 36-37 0.2805775070077985 1106 38-39 0.42960285630957173 1106 40-41 0.472187543027637 1106 42-43 0.403773899838626 1106 44-45 0.48188696385771834 1106 46-47 0.5481830993347998 1106 48-49 0.5685404830164771 1106 50-51 0.5610689851003698 1106 52-53 0.5851689855510216 1106 54-55 0.6706999045018804 1106 56-57 0.671638405169972 1106 58-59 0.6433625121893769 1106 60-61 0.7926953128110412 1106 62-63 0.7586202803805264 1106 64-65 0.6458764699237278 1106 66-67 0.6571293750289513 1106 68-69 0.775345967125773 1106 70-71 0.6614465007424499 1106 72-73 0.6819150351724801 1106 74-75 0.6920890102897168 1106 76-77 0.59381496920982 1106 78-79 0.8300292337623887 1106 80-81 0.7208034874187241 1106 82-83 0.7011087357967973 1106 84-85 0.7629570226114915 1106 86-87 0.8894416796168265 1106 88-89 0.850696113874509 1106 90-91 0.9868100208483845 1106 92-93 0.9456393745612708 1106 94-95 1.0776780797328733 1106 96-97 1.078246552147906 1106 98-99 1.1193318945469706 1106 100-101 1.3122455588734887 1107 1 0.09236999963657766 1107 2 0.09776961727800426 1107 3 0.08496425028467414 1107 4 0.16355216952210583 1107 5 0.16977539801989394 1107 6 0.1570796267311252 1107 7 0.12994296245356196 1107 8 0.144817104242577 1107 9 0.13512247870076521 1107 10-11 0.13182962854744318 1107 12-13 0.25480844236982136 1107 14-15 0.3176943776717849 1107 16-17 0.30506809330720586 1107 18-19 0.21146588382848108 1107 20-21 0.20505000952343977 1107 22-23 0.11167063633368457 1107 24-25 0.12358106324673201 1107 26-27 0.12476360323254454 1107 28-29 0.27786604886345856 1107 30-31 0.31228430222405024 1107 32-33 0.31404709556201027 1107 34-35 0.4457080071401265 1107 36-37 0.37881873316278103 1107 38-39 0.5208973027337791 1107 40-41 0.4036643341852866 1107 42-43 0.4204435570659939 1107 44-45 0.23250609357527452 1107 46-47 0.49446625064127403 1107 48-49 0.5303181894111688 1107 50-51 0.5890903297568428 1107 52-53 0.6449316291397622 1107 54-55 0.6990296115189523 1107 56-57 0.5951421105374237 1107 58-59 0.6286851853240236 1107 60-61 0.620776656898677 1107 62-63 0.5518414112223695 1107 64-65 0.5649763655259719 1107 66-67 0.5102622069519072 1107 68-69 0.6434386084224428 1107 70-71 0.6166813622313967 1107 72-73 0.5490748515729607 1107 74-75 0.6361391747496405 1107 76-77 0.6614322786588183 1107 78-79 0.6439853544990655 1107 80-81 0.5874830887832552 1107 82-83 0.6311502146646184 1107 84-85 0.8056885798445492 1107 86-87 0.9941269889788309 1107 88-89 0.9254706402366146 1107 90-91 0.9173961268288373 1107 92-93 1.0208944296545077 1107 94-95 0.9062146478072357 1107 96-97 0.8803743779140731 1107 98-99 0.9438629411679287 1107 100-101 1.0237616957717357 1108 1 0.23676261132042953 1108 2 0.2660565588587609 1108 3 0.2584444908345027 1108 4 0.26642759907537084 1108 5 0.21950477946319324 1108 6 0.20288976418817128 1108 7 0.3420374641717814 1108 8 0.24068995647625968 1108 9 0.2253681831681149 1108 10-11 0.35324758387390176 1108 12-13 0.4451701262186205 1108 14-15 0.45880863884016776 1108 16-17 0.41373948505978575 1108 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-2.3360294063681373 2316 98-99 -2.513784260105165 2316 100-101 -2.4861534835362598 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9177.0 3 2554.0 4 2674.0 5 2971.0 6 3529.0 7 4159.0 8 5285.0 9 6599.0 10 8224.0 11 10742.0 12 14485.0 13 20605.0 14 18735.0 15 19756.0 16 24797.0 17 33001.0 18 28736.0 19 20427.0 20 19946.0 21 21185.0 22 23380.0 23 26128.0 24 29462.0 25 33388.0 26 39059.0 27 45002.0 28 52796.0 29 62923.0 30 75655.0 31 91367.0 32 113739.0 33 145533.0 34 193691.0 35 276038.0 36 413324.0 37 622137.0 38 567310.0 39 84358.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.71314647812504 19.577425597308476 14.802214689551372 21.907213235015114 2 14.696664714030245 21.480230896891015 40.367240754392725 23.455863634686015 3 20.06414950171668 25.479977651466996 30.562281392764113 23.893591454052217 4 12.268500333637787 17.15043357137561 39.08827756139961 31.49278853358699 5 12.923703811685652 38.336119477959166 34.5427662156457 14.19741049470948 6 29.784118076125836 37.15274394291587 17.89325202244438 15.169885958513913 7 26.799750613453693 33.98052526962476 21.676036532543083 17.54368758437846 8 25.04124959791103 34.147981355686326 20.46781080689007 20.342958239512573 9 25.6004225996184 18.184587728842423 22.027563587050793 34.187426084488386 10-11 24.494569504803867 26.59729281784508 27.67249601891934 21.235641658431714 12-13 26.521944045471034 24.610472044101115 27.73270328735341 21.134880623074434 14-15 22.84417642199597 25.773778955167607 27.720787267412994 23.661257355423427 16-17 20.99221649690169 29.147904849619277 28.0743481199867 21.785530533492338 18-19 20.79651613680496 28.556709126880126 29.96433316838725 20.68244156792767 20-21 22.30034206979031 26.73679423268946 29.381117343878227 21.581746353642007 22-23 21.520856416221438 26.833302446621847 29.714378092778425 21.93146304437829 24-25 22.19799778793865 26.908660037212602 29.63376693353153 21.259575241317215 26-27 21.856970844838187 26.43166219721587 30.657714305723054 21.05365265222289 28-29 20.906438605450923 27.864405485809314 30.14015456085104 21.089001347888722 30-31 21.370580839543493 26.952549598749915 29.778112266752295 21.8987572949543 32-33 21.37389941827972 27.189430630707918 29.90532379401223 21.531346157000137 34-35 22.36714172382098 26.786586636405 29.58292399665216 21.263347643121865 36-37 21.966543664111956 26.430635222485538 30.31265159857655 21.290169514825955 38-39 21.53726645870671 27.19661462601013 30.118820914241617 21.14729800104154 40-41 21.51377859858862 27.341343837826464 29.82877851389369 21.31609904969123 42-43 21.18194672367059 27.518370479155053 29.02663183046664 22.273050966707718 44-45 21.655512091601604 26.89810430293144 29.121929588394597 22.32445401707236 46-47 22.28980228516228 27.489773780019476 28.84921571266816 21.371208222150077 48-49 22.114247401880636 27.32203280299434 28.760345475075745 21.80337432004928 50-51 21.126517633821663 27.744696675966964 29.442983089675657 21.685802600535716 52-53 21.865602969988103 28.302266611549566 28.12248666815546 21.709643750306867 54-55 21.153353993041918 27.389734295538563 28.755520728546124 22.70139098287339 56-57 22.06126796445537 27.523146410845666 28.22742684619484 22.188158778504118 58-59 22.776109105767176 27.191097043360863 28.135192670008376 21.89760118086359 60-61 21.89567921874854 27.400769421028954 29.358582133424015 21.344969226798487 62-63 21.66185536319139 27.44480988885176 29.087393645912346 21.8059411020445 64-65 21.295377624708085 28.17325005733778 28.65501410952846 21.876358208425675 66-67 21.63468164141394 27.46171697410837 28.08335379424507 22.82024759023262 68-69 21.279582590444914 27.560814401098593 28.67520247703053 22.484400531425962 70-71 22.101075319580314 27.418930421625337 28.209645257176387 22.270349001617962 72-73 21.825897243660002 26.874643690064886 29.076142096600126 22.223316969674986 74-75 21.211584067590465 28.424277302635282 28.241411991395953 22.122726638378303 76-77 21.588273358120954 27.28483371927401 28.265569625946362 22.861323296658675 78-79 21.62268375832631 27.34380009858015 28.6209244842157 22.412591658877844 80-81 21.9975244236336 27.504264645035697 28.216851875691674 22.28135905563903 82-83 21.719323805376302 27.203721445821294 28.85110257729918 22.225852171503227 84-85 21.461276374039244 27.472961834200753 28.49869787645781 22.56706391530219 86-87 21.552104885176924 27.528716656254478 28.484040847449172 22.435137611119423 88-89 21.547203870084335 27.3458371586567 28.032882920478198 23.07407605078076 90-91 21.642914334001485 26.738682630557314 28.523311076493467 23.095091958947734 92-93 22.079843676466933 27.376893712891416 28.41471039167846 22.128552218963193 94-95 21.706617038796487 26.950192488588666 28.475511288407816 22.86767918420703 96-97 21.03592288711122 26.92033782270422 29.097101376273653 22.9466379139109 98-99 21.507952549555327 27.30828912786174 28.002846215648358 23.180912106934578 100-101 21.635370847772865 26.054953057063518 29.017943823046938 23.29173227211668 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3464.0 1 2093.5 2 793.5 3 874.5 4 801.0 5 869.0 6 1419.0 7 2781.0 8 4456.0 9 5217.0 10 4837.0 11 4348.5 12 4506.5 13 4892.0 14 5247.0 15 5673.0 16 6151.5 17 6281.0 18 6273.0 19 6579.0 20 7161.5 21 7985.5 22 9346.0 23 11295.0 24 13557.5 25 16553.0 26 20763.0 27 24824.0 28 27597.0 29 31881.0 30 38360.5 31 45134.5 32 51861.0 33 59616.0 34 67792.5 35 73431.0 36 80804.0 37 89452.0 38 92715.0 39 93753.5 40 95536.0 41 98046.5 42 100321.5 43 103809.5 44 107564.5 45 109793.5 46 113343.5 47 133456.0 48 183335.5 49 181362.0 50 129824.5 51 106481.5 52 96891.0 53 91477.5 54 86246.5 55 78538.0 56 70818.0 57 62539.0 58 54828.5 59 47669.0 60 40721.5 61 33816.0 62 27190.5 63 21205.5 64 15754.0 65 11812.0 66 8501.5 67 6480.0 68 4791.0 69 2979.5 70 2047.0 71 1503.5 72 1148.0 73 901.5 74 675.5 75 553.5 76 395.0 77 235.5 78 147.0 79 107.0 80 74.0 81 54.0 82 41.5 83 29.5 84 26.5 85 23.0 86 17.5 87 12.5 88 9.5 89 7.0 90 6.0 91 5.0 92 4.5 93 4.5 94 3.5 95 4.5 96 3.5 97 3.0 98 3.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3268313745512766 2 0.08878341196826867 3 0.09792334433276917 4 0.10353337178408328 5 0.11459584164594423 6 0.058337982092035966 7 0.06016596856493607 8 0.061458165899227514 9 0.06429469663303802 10-11 0.061599992435918044 12-13 0.06002414202824554 14-15 0.05816463854719199 16-17 0.059519869897790345 18-19 0.06798218658699168 20-21 0.06908528187236242 22-23 0.08517471453469866 24-25 0.09442495642773623 26-27 0.08985499024548597 28-29 0.08657722139752717 30-31 0.08030533677499062 32-33 0.07230001670401433 34-35 0.32314388459732296 36-37 0.5014198412173129 38-39 0.4749770713765683 40-41 0.45685479168833465 42-43 0.48682746644226554 44-45 0.4960777083353031 46-47 0.49056223190844944 48-49 0.5085426850599926 50-51 0.5043351644715071 52-53 0.5034842052513638 54-55 0.4947539939928583 56-57 0.4745515917664968 58-59 0.4577372679166312 60-61 0.41733246346390823 62-63 0.48520434052236294 64-65 0.5092518177434452 66-67 0.5189275392465543 68-69 0.531156138410093 70-71 0.528177781139592 72-73 0.5169892432451173 74-75 0.5190378487750915 76-77 0.4938400007564082 78-79 0.4744412822379597 80-81 0.48036647977080826 82-83 0.4889863815007769 84-85 0.49001068426576405 86-87 0.4895379291434623 88-89 0.4963771199127609 90-91 0.5100239844432048 92-93 0.5156024948863654 94-95 0.5231508183391167 96-97 0.5144521240887645 98-99 0.5174462398633423 100-101 0.49667653149021873 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3172890.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.89389769163352 #Duplication Level Percentage of deduplicated Percentage of total 1 69.34955346475988 29.746726512778785 2 15.073468650810854 12.931196443318518 3 5.990964049903515 7.709273970924474 4 3.0691713927650124 5.2659489487740325 5 1.7229925038583 3.69529320919747 6 1.1045540698438243 2.842717756005506 7 0.7264921726040134 2.181345664781438 8 0.5143664996544746 1.7650547209746146 9 0.38568243079309744 1.4889080455109682 >10 1.8372767826620544 13.861786579170236 >50 0.1379255012116939 4.108595415903928 >100 0.07981309814796855 6.2782412995651375 >500 0.004201379335015115 1.2081578643208166 >1k 0.002432377509609608 2.195633188754854 >5k 6.63375684438984E-4 1.823476430052648 >10k+ 4.42250456292656E-4 2.8976439499666267 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 27095 0.853953335917728 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15706 0.49500613005808586 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14700 0.4633000198557152 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12441 0.3921030984370716 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11329 0.35705618537043515 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10379 0.32711502762465766 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8054 0.25383798366788635 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7331 0.23105118677294204 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6778 0.21362228126408414 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6627 0.2088632130329132 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6427 0.2025598114022232 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5880 0.18532000794228606 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5787 0.1823889261840152 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5757 0.1814434159394117 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5034 0.15865661904446735 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4288 0.13514493096199365 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3888 0.12253812770061363 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3796 0.11963856295049624 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3676 0.11585652197208224 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3642 0.11478494369486494 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3251 0.10246179350686599 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.151700815345001E-5 0.0 4 0.0 0.0 0.0 6.303401630690002E-5 0.0 5 0.0 0.0 0.0 6.303401630690002E-5 0.0 6 0.0 0.0 0.0 6.303401630690002E-5 0.0 7 0.0 0.0 0.0 6.303401630690002E-5 0.0 8 0.0 0.0 0.0 6.303401630690002E-5 0.0 9 0.0 0.0 0.0 1.8910204892070006E-4 0.0 10-11 0.0 0.0 0.0 2.0486055299742505E-4 0.0 12-13 0.0 0.0 0.0 2.521360652276001E-4 0.0 14-15 0.0 0.0 0.0 2.678945693043251E-4 0.0 16-17 0.0 0.0 0.0 3.782040978414001E-4 0.0 18-19 0.0 0.0 0.0 5.042721304552001E-4 0.0 20-21 0.0 0.0 0.0 6.776156752991752E-4 0.0 22-23 0.0 0.0 0.0 0.0011976463098311004 0.0 24-25 0.0 0.0 0.0 0.0017176769443630255 0.0 26-27 0.0 0.0 0.0 0.0025843946685829007 0.0 28-29 0.0 0.0 0.0 0.005657302963544277 0.0 30-31 1.5758504076725006E-5 0.0 0.0 0.015033612889195655 0.0 32-33 3.151700815345001E-5 0.0 0.0 0.03277768847958801 0.0 34-35 3.151700815345001E-5 0.0 0.0 0.05758157389635317 0.0 36-37 3.151700815345001E-5 0.0 0.0 0.09440919792365951 0.0 38-39 3.151700815345001E-5 0.0 0.0 0.15044643842049363 0.0 40-41 3.151700815345001E-5 0.0 0.0 0.21322831866216602 0.0 42-43 3.151700815345001E-5 0.0 0.0 0.2794297942884878 0.0 44-45 3.151700815345001E-5 0.0 0.0 0.36279228085436305 0.0 46-47 3.151700815345001E-5 0.0 0.0 0.4629690912701039 0.0 48-49 3.151700815345001E-5 0.0 0.0 0.5650999561913587 0.0 50-51 3.151700815345001E-5 0.0 0.0 0.6800582434310676 0.0 52-53 3.151700815345001E-5 0.0 0.0 0.7990349492103413 0.0 54-55 3.151700815345001E-5 0.0 0.0 0.9311542473896037 0.0 56-57 3.151700815345001E-5 0.0 0.0 1.0977216354805872 1.5758504076725006E-5 58-59 3.151700815345001E-5 0.0 0.0 1.275776973043503 3.151700815345001E-5 60-61 3.151700815345001E-5 0.0 0.0 1.4793453287066365 3.151700815345001E-5 62-63 3.151700815345001E-5 0.0 0.0 1.6842216402081385 3.151700815345001E-5 64-65 3.151700815345001E-5 0.0 0.0 1.896945686739849 3.151700815345001E-5 66-67 3.151700815345001E-5 0.0 0.0 2.146560391315173 3.151700815345001E-5 68-69 3.151700815345001E-5 0.0 0.0 2.4397316011585652 3.151700815345001E-5 70-71 3.151700815345001E-5 0.0 0.0 2.7566508766455815 3.151700815345001E-5 72-73 3.151700815345001E-5 0.0 0.0 3.0800941728203624 3.151700815345001E-5 74-75 3.151700815345001E-5 0.0 0.0 3.431177885145719 3.151700815345001E-5 76-77 3.151700815345001E-5 3.151700815345001E-5 0.0 3.789478992338215 3.151700815345001E-5 78-79 3.151700815345001E-5 3.151700815345001E-5 0.0 4.2281011948097795 3.151700815345001E-5 80-81 3.151700815345001E-5 3.151700815345001E-5 0.0 4.705237181244859 3.151700815345001E-5 82-83 3.151700815345001E-5 3.151700815345001E-5 0.0 5.170964010728389 3.151700815345001E-5 84-85 3.151700815345001E-5 3.151700815345001E-5 0.0 5.668570293959135 3.151700815345001E-5 86-87 3.151700815345001E-5 3.151700815345001E-5 0.0 6.189609472751971 3.151700815345001E-5 88-89 3.151700815345001E-5 3.151700815345001E-5 0.0 6.747129588482425 3.151700815345001E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 990 0.0 28.228176 3 CTTTGCG 1070 0.0 26.117657 2 TTGCGTT 1125 0.0 25.261827 4 GTACATG 14210 0.0 23.249525 1 GAGTACT 11710 0.0 22.72183 12-13 TACATGG 14550 0.0 22.331974 2 ACATGGG 14545 0.0 21.069613 3 AGTACTT 12185 0.0 20.96186 12-13 GTACTTT 12790 0.0 20.637255 14-15 CATGGGG 5070 0.0 19.805868 4 ACTTTTT 15280 0.0 17.616753 16-17 CATGGGA 9325 0.0 17.371738 4 GTATAGG 960 0.0 17.305964 1 TACTTTT 14905 0.0 16.994148 14-15 GTAAGCG 300 0.0 16.647154 94-95 AGAGTAC 25660 0.0 16.401943 10-11 TATAGCG 210 4.0934E-4 15.788392 5 GTCGGGA 675 0.0 15.437782 2 ATGGGAT 3905 0.0 15.040415 5 GTCCTAT 830 0.0 14.869425 1 >>END_MODULE