FastQCFastQC Report
Fri 27 May 2016
SRR938989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172890
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT301310.9496389726716021No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144110.4541916044993681No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT143280.4515756928226317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122260.3853269416840798No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT110740.34901934829130543No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC93670.2952198153733663No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT85880.2706680660218287No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC76490.2410735953657391No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74880.23599935705303368No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC73680.23221731607461965No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG67960.21418958741084626No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT67440.21255070298686687No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA64270.2025598114022232No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC53850.1697190889063283No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA50370.1587511700689277No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA49670.15654497949818622No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA40660.12814815515192773No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC37900.11944946090157553No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA36200.11409156951548904No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT35500.11188537894474754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG9600.027.187532
TTTGCGT9450.027.1169153
GTAAGCG5750.025.99727694-95
TTGCGTT10550.024.2895574
GAGTACT125300.022.97129212-13
GTACATG143950.022.2397381
TACATGG147750.021.069522
GTACTTT136350.020.98784414-15
AGTACTT131100.020.8871312-13
ACATGGG147300.020.3606973
CATGGGG51950.019.1828024
ACTTTTT159400.018.05639516-17
ACCGTAT1350.0094558617.5757798
TACTTTT157450.017.1504414-15
AATTCGC3200.017.05466878-79
ATTCGCG3100.016.85280480-81
CGCGGAA3150.016.67194282-83
CATGGGA94250.016.6657544
TAGACTG11500.016.5059475
GTATAGG8100.016.468161
Produced by FastQC (version 0.11.5)