##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938989_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3172890 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17068193350541 33.0 31.0 34.0 30.0 34.0 2 32.39966119216235 34.0 31.0 34.0 31.0 34.0 3 32.48896116789425 34.0 31.0 34.0 30.0 34.0 4 36.03223969315041 37.0 35.0 37.0 35.0 37.0 5 35.95930303288169 37.0 35.0 37.0 35.0 37.0 6 36.016666509081624 37.0 35.0 37.0 35.0 37.0 7 35.95721187939071 37.0 35.0 37.0 35.0 37.0 8 35.934479922089956 37.0 35.0 37.0 35.0 37.0 9 37.599827917135485 39.0 37.0 39.0 35.0 39.0 10-11 37.510097103902126 39.0 37.0 39.0 35.0 39.0 12-13 37.4250618521285 39.0 37.0 39.0 34.0 39.0 14-15 38.69351852727324 40.0 38.0 41.0 34.0 41.0 16-17 38.8093687458437 40.0 38.0 41.0 34.5 41.0 18-19 38.878072356747325 40.0 38.0 41.0 35.0 41.0 20-21 38.85813847943042 40.0 38.0 41.0 34.5 41.0 22-23 38.779239904314366 40.0 38.0 41.0 34.0 41.0 24-25 38.69656086407029 40.0 38.0 41.0 34.0 41.0 26-27 38.527184522627635 40.0 38.0 41.0 34.0 41.0 28-29 38.37840848563928 40.0 38.0 41.0 34.0 41.0 30-31 38.18932424382818 40.0 38.0 41.0 34.0 41.0 32-33 38.07088836990882 40.0 37.0 41.0 33.0 41.0 34-35 37.9431352804541 40.0 37.0 41.0 33.0 41.0 36-37 37.79894465298199 40.0 37.0 41.0 33.0 41.0 38-39 37.65100381670969 40.0 37.0 41.0 33.0 41.0 40-41 37.501635102383 40.0 37.0 41.0 32.5 41.0 42-43 37.30281084437216 40.0 37.0 41.0 32.0 41.0 44-45 37.02798032708351 40.0 36.0 41.0 31.0 41.0 46-47 36.677116761060105 40.0 36.0 41.0 30.5 41.0 48-49 36.31806365805306 39.0 36.0 41.0 30.0 41.0 50-51 36.046688823123404 39.0 35.0 41.0 29.0 41.0 52-53 35.80410414480174 39.0 35.0 40.0 28.5 41.0 54-55 35.52217268799107 39.0 35.0 40.0 28.0 41.0 56-57 35.38188071442754 38.5 35.0 40.0 27.5 41.0 58-59 35.11403200237008 38.0 34.5 40.0 27.0 41.0 60-61 34.73260308425442 38.0 34.0 40.0 26.0 41.0 62-63 34.3351747145347 37.5 34.0 40.0 26.0 41.0 64-65 33.93144357352445 37.0 33.0 39.5 25.5 41.0 66-67 33.46265076948775 36.0 33.0 39.0 23.5 40.5 68-69 32.9610421413916 36.0 32.0 39.0 22.5 40.0 70-71 32.40598460709322 35.0 31.5 38.0 21.5 40.0 72-73 31.8780146806224 35.0 31.0 37.0 20.5 39.5 74-75 31.35226307877046 35.0 31.0 37.0 18.5 39.0 76-77 29.446772028024924 32.5 28.5 35.0 13.5 37.0 78-79 30.410067477914456 34.0 30.0 36.0 15.5 38.0 80-81 30.363139913454297 34.0 30.0 35.5 13.0 37.0 82-83 29.93487892741318 34.0 30.0 35.0 7.5 37.0 84-85 29.465942720989382 34.0 29.0 35.0 4.5 36.0 86-87 28.778951366104717 34.0 29.0 35.0 2.0 36.0 88-89 28.059737179669007 33.0 27.5 35.0 2.0 35.0 90-91 27.454846055173675 33.0 26.0 35.0 2.0 35.0 92-93 27.044436617720756 33.0 26.0 34.0 2.0 35.0 94-95 26.468559105421242 32.0 25.0 34.0 2.0 35.0 96-97 25.570310978319448 32.0 23.5 34.0 2.0 35.0 98-99 24.333179845503626 31.5 10.0 34.0 2.0 35.0 100-101 18.7258623841356 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.056347975633947556 1101 2 0.09156199167170342 1101 3 0.09135176470670103 1101 4 0.06395165453730556 1101 5 0.029745077403987352 1101 6 0.04344932819417835 1101 7 0.11930139367768078 1101 8 0.1314838016420552 1101 9 0.18456674299569897 1101 10-11 0.1926527133978695 1101 12-13 0.20136264481965327 1101 14-15 0.26407749835822614 1101 16-17 0.21214494074578027 1101 18-19 0.2677684956375401 1101 20-21 0.29567201597362924 1101 22-23 0.2811169768006394 1101 24-25 0.20055631445161026 1101 26-27 0.24293814201962505 1101 28-29 0.17894423278796978 1101 30-31 0.1538439985074973 1101 32-33 0.2523310843033215 1101 34-35 0.3066246433878774 1101 36-37 0.4538320754522047 1101 38-39 0.3958253874626294 1101 40-41 0.40712524505646996 1101 42-43 0.32796730529037177 1101 44-45 0.40172425252132626 1101 46-47 0.2761026654895389 1101 48-49 0.2216160882370133 1101 50-51 0.12513972215258917 1101 52-53 0.1590030039749024 1101 54-55 0.2274300760224719 1101 56-57 0.3252945517000043 1101 58-59 0.2739346638575526 1101 60-61 0.30126058217676643 1101 62-63 0.33537403178046077 1101 64-65 0.26816537690231 1101 66-67 0.15150546235850015 1101 68-69 0.297909851038888 1101 70-71 0.33634034276195734 1101 72-73 0.3088649970223365 1101 74-75 0.32823046050405935 1101 76-77 -0.024590176170057987 1101 78-79 0.3610374559126015 1101 80-81 0.43179731518356945 1101 82-83 0.3342350431695067 1101 84-85 0.3993247911491835 1101 86-87 0.6876654718283604 1101 88-89 0.6007465623468349 1101 90-91 0.5253450412444529 1101 92-93 0.5320275223333297 1101 94-95 0.48064546282162013 1101 96-97 0.5592346194214457 1101 98-99 0.8783012592675767 1101 100-101 1.2395839932470665 1102 1 0.14299701465107262 1102 2 0.12536499320162164 1102 3 0.15727365455631315 1102 4 0.1048043360270654 1102 5 0.08828025767343917 1102 6 0.12774536921727275 1102 7 0.13348595965286592 1102 8 0.13049099586184099 1102 9 0.21176989734457408 1102 10-11 0.30460501870461343 1102 12-13 0.21296653670110288 1102 14-15 0.30509610742974047 1102 16-17 0.27813841058128475 1102 18-19 0.17471416901548054 1102 20-21 0.26145954242803526 1102 22-23 0.2574183095682301 1102 24-25 0.26958253342427696 1102 26-27 0.20653756317038585 1102 28-29 0.17722148363355927 1102 30-31 0.14842754959922644 1102 32-33 0.3041955273334125 1102 34-35 0.22746908024311807 1102 36-37 0.24622458904809008 1102 38-39 0.2794308109832926 1102 40-41 0.21328543589043392 1102 42-43 0.20612609557136352 1102 44-45 0.18477255952529958 1102 46-47 0.25549349393732257 1102 48-49 0.24210883451097232 1102 50-51 0.24593482918623266 1102 52-53 0.17820679305715004 1102 54-55 0.24431036151450058 1102 56-57 0.22650623700334194 1102 58-59 0.07559255981993118 1102 60-61 0.2017926888188697 1102 62-63 0.33908704950641777 1102 64-65 0.26534167582033774 1102 66-67 0.22668756011778157 1102 68-69 0.1994663276477624 1102 70-71 0.25401680467536636 1102 72-73 0.215418619055864 1102 74-75 0.22649994966443643 1102 76-77 0.05421904333212524 1102 78-79 0.3074671669536855 1102 80-81 0.35168482249249067 1102 82-83 0.35258366702271715 1102 84-85 0.6165950582029005 1102 86-87 0.7865260564384933 1102 88-89 0.5600920625529504 1102 90-91 0.6488529435024155 1102 92-93 0.5651293849652994 1102 94-95 0.7379534344173031 1102 96-97 0.9211809741028603 1102 98-99 0.9732104561746304 1102 100-101 1.1909665218661303 1103 1 0.07199412395019777 1103 2 0.05176525020398515 1103 3 0.08570367754489894 1103 4 0.0688603906377594 1103 5 0.1209357933282007 1103 6 0.0792784309249015 1103 7 0.09356527113524038 1103 8 0.1329507111442183 1103 9 0.16558235815948308 1103 10-11 0.14328001722213202 1103 12-13 0.17263524315131917 1103 14-15 0.17882060941569478 1103 16-17 0.18651142556329603 1103 18-19 0.17034029275313145 1103 20-21 0.1989381903330738 1103 22-23 0.15857297789758462 1103 24-25 0.21354571253891663 1103 26-27 0.0873368842788409 1103 28-29 0.16613837180932478 1103 30-31 0.22444688004588187 1103 32-33 0.1849318068672403 1103 34-35 0.2614170545359755 1103 36-37 0.1867888773490094 1103 38-39 0.18140392591067211 1103 40-41 0.3092230828794911 1103 42-43 0.43775983956730613 1103 44-45 0.3824537810234574 1103 46-47 0.46901409816922524 1103 48-49 0.3692222961036862 1103 50-51 0.36088322433197106 1103 52-53 0.471042564285888 1103 54-55 0.6630225401679866 1103 56-57 0.7388614644740343 1103 58-59 0.6424955028539472 1103 60-61 0.6006172369201721 1103 62-63 0.5561439118552869 1103 64-65 0.5497060411528807 1103 66-67 0.458961161703094 1103 68-69 0.4315041134434239 1103 70-71 0.44320957890190016 1103 72-73 0.3399565300710954 1103 74-75 0.3838178098894609 1103 76-77 0.24467587309511885 1103 78-79 0.41012447007542363 1103 80-81 0.3810694503658496 1103 82-83 0.4552829978608699 1103 84-85 0.533096636545153 1103 86-87 0.5977104953598449 1103 88-89 0.41271594500430453 1103 90-91 0.6283317883044184 1103 92-93 0.7220790133078694 1103 94-95 0.843371440357604 1103 96-97 0.7107542430934899 1103 98-99 0.7836027589998729 1103 100-101 0.9312211292265076 1104 1 0.05286767418171934 1104 2 0.024473219862400697 1104 3 0.05650119814492882 1104 4 0.07750236994157689 1104 5 0.08341274774185337 1104 6 0.09369544462373369 1104 7 0.08394460564713313 1104 8 0.10496280580078121 1104 9 0.1082076318402656 1104 10-11 0.17966921764892874 1104 12-13 0.16072319453969897 1104 14-15 0.1727111597544635 1104 16-17 0.13230772107197453 1104 18-19 0.11757670625890881 1104 20-21 0.1723110031982955 1104 22-23 0.15506881212468926 1104 24-25 0.20703764589024587 1104 26-27 0.07062259314905361 1104 28-29 0.1987842825006254 1104 30-31 0.08068657078659669 1104 32-33 0.1610086043767467 1104 34-35 0.1693000088460721 1104 36-37 0.19931780568533242 1104 38-39 0.21237469754255045 1104 40-41 0.23085439371799765 1104 42-43 0.16197883227408028 1104 44-45 0.36556052253935434 1104 46-47 0.3448894606113413 1104 48-49 0.34828773863441853 1104 50-51 0.38237051759202245 1104 52-53 0.19517111982460023 1104 54-55 0.5041618820581633 1104 56-57 0.39637359750138756 1104 58-59 0.4383622873451216 1104 60-61 0.4681840529401171 1104 62-63 0.4582644294078406 1104 64-65 0.3985196557548534 1104 66-67 0.41164368413382135 1104 68-69 0.5281125058276999 1104 70-71 0.4700794150262766 1104 72-73 0.4190146220537265 1104 74-75 0.42343501044939913 1104 76-77 0.30558365885114114 1104 78-79 0.5324652128228813 1104 80-81 0.6239329465269208 1104 82-83 0.4839242467328617 1104 84-85 0.5072005168948728 1104 86-87 0.6564050842469236 1104 88-89 0.6066162960738808 1104 90-91 0.5929250302382343 1104 92-93 0.621993441832803 1104 94-95 1.0207225986930304 1104 96-97 0.8784587894306028 1104 98-99 1.0804767766152956 1104 100-101 1.0607268649655168 1105 1 0.00968260553182887 1105 2 0.029586206132421466 1105 3 0.00871801736689548 1105 4 -0.033860616328446724 1105 5 -0.030199237384003652 1105 6 0.005516096797649084 1105 7 0.11674637910250851 1105 8 0.134264150194376 1105 9 0.14748823824758972 1105 10-11 0.08601754430796404 1105 12-13 0.030202599574025157 1105 14-15 -0.04589045637825251 1105 16-17 0.005909694756191186 1105 18-19 0.032252407059829125 1105 20-21 -0.014511436733059213 1105 22-23 -0.037739039705975586 1105 24-25 -0.0907723512493348 1105 26-27 0.07310042495683433 1105 28-29 0.06173479737476839 1105 30-31 0.052225472388428784 1105 32-33 0.05733833835843427 1105 34-35 0.022736078640129165 1105 36-37 0.050499513351233816 1105 38-39 0.23081005223362894 1105 40-41 0.24287706248230023 1105 42-43 0.2967185347912391 1105 44-45 0.3894860803196778 1105 46-47 0.2755459251422394 1105 48-49 0.20857985819963432 1105 50-51 0.2630949169055299 1105 52-53 0.22000843389782432 1105 54-55 0.290176255627955 1105 56-57 0.19170933834806902 1105 58-59 0.298858211258171 1105 60-61 0.20455061472500802 1105 62-63 0.27700727837809325 1105 64-65 0.1561623187983372 1105 66-67 0.20912115839011847 1105 68-69 0.2387031808849116 1105 70-71 0.4100572468340573 1105 72-73 0.347913721023982 1105 74-75 0.25549128905351637 1105 76-77 0.054384073610869166 1105 78-79 0.34205116820045944 1105 80-81 0.3077144110578125 1105 82-83 0.35527007053148907 1105 84-85 0.3650666496179866 1105 86-87 0.5621831162835385 1105 88-89 0.621374233716903 1105 90-91 0.5724444810391489 1105 92-93 0.8380761077366934 1105 94-95 0.812470310363512 1105 96-97 0.8507503369134426 1105 98-99 1.0955243309631228 1105 100-101 1.167093069198927 1106 1 0.09401463204407179 1106 2 0.07037098209374903 1106 3 0.06090406000513582 1106 4 0.012759265288934785 1106 5 0.05820069684787654 1106 6 0.030333526203683903 1106 7 0.05877786665781315 1106 8 0.07054968206202261 1106 9 0.058448986637550604 1106 10-11 0.18865051121891696 1106 12-13 0.11334877135693944 1106 14-15 0.010236175012778403 1106 16-17 -0.06540355367648232 1106 18-19 -0.0300953973953213 1106 20-21 -0.0375397277014784 1106 22-23 0.020052400615867327 1106 24-25 0.10713972446087183 1106 26-27 0.05160978410201977 1106 28-29 0.01372406948396332 1106 30-31 0.05312524647066397 1106 32-33 0.11284177410212948 1106 34-35 -1.4391375810163254E-4 1106 36-37 0.053143530587611565 1106 38-39 0.08930844646652503 1106 40-41 0.30426535703806223 1106 42-43 0.16309586443472313 1106 44-45 0.17491123491746663 1106 46-47 0.20507015258036176 1106 48-49 0.28933590331485703 1106 50-51 0.1850779645318994 1106 52-53 0.2891856597643425 1106 54-55 0.24037532163523423 1106 56-57 0.1719112656956483 1106 58-59 0.16174363806764092 1106 60-61 0.382510927507937 1106 62-63 0.43385751924315485 1106 64-65 0.3039633574221625 1106 66-67 0.25484062532668617 1106 68-69 0.2983619660537755 1106 70-71 0.23310774521975475 1106 72-73 0.24661522771182476 1106 74-75 0.3207468238252069 1106 76-77 0.2426556212116111 1106 78-79 0.3655157601818715 1106 80-81 0.4517022866621119 1106 82-83 0.3938040500152553 1106 84-85 0.4447278583847911 1106 86-87 0.5158965060749559 1106 88-89 0.6772504444482088 1106 90-91 0.9321471472104186 1106 92-93 0.9054887514443095 1106 94-95 0.6691291190209903 1106 96-97 0.7587938527746623 1106 98-99 0.8806549703659137 1106 100-101 1.335934781561626 1107 1 0.022647201299598407 1107 2 0.095104383017933 1107 3 0.031554425397281705 1107 4 0.10706450575511184 1107 5 0.08374541806906421 1107 6 0.07239754009661681 1107 7 0.051468978125839726 1107 8 0.15998647792090992 1107 9 0.05763473851888534 1107 10-11 0.08779792954941712 1107 12-13 0.1364627732039736 1107 14-15 0.16559726744160486 1107 16-17 0.22935991179362247 1107 18-19 0.17498334845971897 1107 20-21 0.21621123011447452 1107 22-23 0.24042326360385147 1107 24-25 0.19847751310226158 1107 26-27 0.2918063392270511 1107 28-29 0.20274130849793437 1107 30-31 0.2582414788995564 1107 32-33 0.3163148711853552 1107 34-35 0.28868663479856593 1107 36-37 0.37403451063826765 1107 38-39 0.3176385926568486 1107 40-41 0.2769502447937455 1107 42-43 0.3861566112969399 1107 44-45 0.35097380413953516 1107 46-47 0.42859631852259383 1107 48-49 0.37169197160169887 1107 50-51 0.3468798463683598 1107 52-53 0.3937350345489392 1107 54-55 0.44616343685422066 1107 56-57 0.5449564219074929 1107 58-59 0.4554948091963098 1107 60-61 0.46558222080206235 1107 62-63 0.6139121210259475 1107 64-65 0.6083208239529796 1107 66-67 0.5720450596380857 1107 68-69 0.5601118895121502 1107 70-71 0.5279255322382923 1107 72-73 0.5247670172800802 1107 74-75 0.5129706026726168 1107 76-77 0.5245770334589395 1107 78-79 0.7872661709134263 1107 80-81 0.6533550106237378 1107 82-83 0.5691859006996935 1107 84-85 0.7203196338827702 1107 86-87 0.7675870093925958 1107 88-89 1.075830283635515 1107 90-91 1.0422050616143004 1107 92-93 1.1328349927068508 1107 94-95 1.2019473857840204 1107 96-97 1.1180777243252678 1107 98-99 1.2565101557053922 1107 100-101 1.286968950913522 1108 1 0.041007166935337125 1108 2 0.04088445174645727 1108 3 0.08261714017390887 1108 4 -0.021742195275813003 1108 5 0.04369988542301684 1108 6 0.04434943013097836 1108 7 0.09525076506741925 1108 8 0.12233871159788379 1108 9 0.10767683473881817 1108 10-11 0.01824122851849097 1108 12-13 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-0.9078531981378077 2316 60-61 -0.7721695235789312 2316 62-63 -0.7010568400361663 2316 64-65 -0.5689925598800301 2316 66-67 -0.7066548143566678 2316 68-69 -0.6788146675241862 2316 70-71 -0.7267446952630507 2316 72-73 -0.6623167629039592 2316 74-75 -0.8300752748135594 2316 76-77 -0.7192008753063419 2316 78-79 -0.9010514660956588 2316 80-81 -0.9325199108724611 2316 82-83 -0.8984058356446383 2316 84-85 -1.0318333992910702 2316 86-87 -1.2943869153714935 2316 88-89 -1.3646272471406355 2316 90-91 -1.551175621262292 2316 92-93 -1.4363901382215154 2316 94-95 -1.47973684666173 2316 96-97 -1.2569668498648099 2316 98-99 -1.323274068623867 2316 100-101 -0.9107939237092602 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 15.0 4 23.0 5 61.0 6 165.0 7 393.0 8 955.0 9 1799.0 10 3171.0 11 4731.0 12 7216.0 13 9909.0 14 12431.0 15 16547.0 16 22159.0 17 31718.0 18 31004.0 19 20927.0 20 19125.0 21 20292.0 22 21873.0 23 24016.0 24 27980.0 25 32690.0 26 39123.0 27 47455.0 28 57174.0 29 69381.0 30 83984.0 31 102070.0 32 126037.0 33 160502.0 34 215250.0 35 307425.0 36 475392.0 37 704453.0 38 453502.0 39 21936.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66902251418587 20.244098541844718 15.345610614983151 21.74126832898626 2 14.955230089918023 22.158978092527633 39.70153393278683 23.184257884767515 3 20.032399484381745 25.742997708713506 30.75401290306314 23.470589903841606 4 12.57077932106061 17.811521987840738 38.54419787638399 31.07350081471466 5 12.781123833476734 38.58655673534223 34.558998263412846 14.073321167768185 6 29.625640977153324 37.76200876803167 17.507351342151793 15.10499891266322 7 26.734869472310734 34.1104166863648 21.53821909993728 17.61649474138719 8 25.107331171266573 34.591208645745674 20.13574375411691 20.165716428870837 9 25.418372524732973 18.285254137395246 21.84872466426507 34.447648673606714 10-11 24.51367680568819 26.93538067818298 27.45208626835472 21.098856247774112 12-13 26.547327515293627 24.744932853014127 27.670420342337742 21.0373192893545 14-15 22.678092758729914 26.065909094490607 27.64626810869075 23.609730038088724 16-17 20.894601083830107 29.35604329521164 28.03469592684367 21.714659694114577 18-19 20.698337597600663 28.675354047650114 29.97501006972219 20.651298285027032 20-21 22.24377460296449 26.793097144874235 29.462367116414374 21.500761135746906 22-23 21.513604316569438 26.88424433245401 29.756846282096134 21.84530506888042 24-25 22.156020684001668 27.0404681919022 29.753234199642154 21.050276924453975 26-27 21.824939640961226 26.56089238559754 30.725331192697126 20.888836780744107 28-29 20.8126030212204 28.02501820107221 30.174556949657884 20.98782182804951 30-31 21.37332211327843 27.020965113823674 29.794477589831352 21.81123518306654 32-33 21.360116486862136 27.27566981521578 29.920214693859542 21.44399900406254 34-35 22.34109401662524 26.821300341958015 29.739566907819803 21.098038733596947 36-37 21.9476379910022 26.53214312622927 30.385667705617937 21.134551177150595 38-39 21.46177948486232 27.28746027815966 30.254319068012116 20.99644116896591 40-41 21.485530737156566 27.44462035540177 29.816739165585304 21.25310974185636 42-43 21.185611177011317 27.61528609541352 29.061471261389848 22.137631466185315 44-45 21.71743125974386 26.949776563930357 29.093177497765012 22.239614678560773 46-47 22.31793182785193 27.64015002285012 28.79973840553446 21.242179743763494 48-49 22.089848366894753 27.497974968403206 28.730943428339096 21.68123323636295 50-51 21.118013205396565 27.863295354567846 29.331130700459003 21.68756073957659 52-53 21.913287255467413 28.37473092354289 28.020842197491874 21.691139623497822 54-55 21.225712205591748 27.471957741995467 28.578551415271253 22.723778637141535 56-57 22.119235145246133 27.608394870291754 28.14174144076851 22.130628543693604 58-59 22.826004737669514 27.27373152617758 28.06495574351029 21.83530799264262 60-61 21.88918040816095 27.59712390297467 29.2241197677224 21.289575921141978 62-63 21.663903112882533 27.59299455662375 28.948354585014656 21.79474774547906 64-65 21.284297696480703 28.28508956817385 28.50003742677157 21.930575308573882 66-67 21.64943189796147 27.58757520758863 27.948675642015424 22.81431725243448 68-69 21.26804100154201 27.744860033267067 28.420681052979102 22.56641791221182 70-71 22.07486963377377 27.63894791764665 28.017735259212547 22.268447189367038 72-73 21.791931015572555 27.03979652619536 28.848746726170777 22.319525732061308 74-75 21.11415775523261 28.648298554314838 27.931570271897225 22.305973418555322 76-77 21.531947016988703 27.421138976696707 28.105390620921185 22.94152338539341 78-79 21.541025406661017 27.537955553314276 28.39965771450364 22.52136132552107 80-81 21.90773051612415 27.649170469730972 28.059271072338067 22.383827941806814 82-83 21.608486269615398 27.372805234344717 28.649023445502365 22.369685050537523 84-85 21.246210268987184 27.705965252112158 28.285186014551115 22.762638464349543 86-87 21.307279768665595 27.746500622327154 28.168651525659737 22.777568083347514 88-89 21.25383855423136 27.57837994572448 27.746879062710605 23.420902437333556 90-91 21.407109489607677 26.945605052999 28.237631180494333 23.40965427689899 92-93 21.761391034671863 27.70912322835018 28.016808020448238 22.512677716529726 94-95 21.34186718271084 27.257060281670608 28.119154515412774 23.281918020205776 96-97 20.53613906188665 27.296238964514835 28.723896624827304 23.443725348771206 98-99 21.02094620361878 27.640983456722417 27.603950972142115 23.734119367516683 100-101 21.004348899726953 26.44186819415788 28.76577258171344 23.78801032440173 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1633.0 1 1114.5 2 662.5 3 730.0 4 643.0 5 686.5 6 1160.5 7 2357.5 8 3833.5 9 4604.5 10 4251.0 11 3682.0 12 3796.5 13 4067.0 14 4327.5 15 4681.0 16 5005.5 17 5217.5 18 5486.5 19 5972.0 20 6609.5 21 7780.5 22 9398.5 23 11297.0 24 13595.0 25 16491.5 26 20730.0 27 24897.0 28 27964.0 29 32636.0 30 39011.0 31 45710.0 32 52791.5 33 60433.0 34 68192.5 35 73979.0 36 81639.0 37 90122.0 38 93210.0 39 94147.5 40 95868.5 41 98442.5 42 100518.5 43 104233.5 44 108460.5 45 110281.5 46 112354.5 47 134330.0 48 186046.5 49 182850.0 50 131691.5 51 108643.0 52 97937.0 53 91872.0 54 86492.0 55 78561.5 56 70714.0 57 62575.0 58 55178.0 59 47569.0 60 40159.0 61 33078.5 62 26534.0 63 20746.5 64 15314.0 65 11195.5 66 7858.5 67 6079.0 68 4406.5 69 2617.5 70 1777.5 71 1319.5 72 1004.0 73 761.0 74 571.0 75 464.5 76 327.5 77 153.0 78 66.5 79 43.0 80 26.5 81 12.5 82 7.0 83 4.5 84 3.5 85 3.0 86 2.5 87 1.0 88 0.5 89 0.5 90 1.5 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39481356113826827 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011030952853707504 16-17 0.0021431565544346005 18-19 3.782040978414001E-4 20-21 0.0 22-23 0.0 24-25 9.455102446035003E-5 26-27 4.7275512230175014E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013378969961139528 36-37 0.024614783367844457 38-39 0.01133036443116528 40-41 0.006271884622536552 42-43 0.005153030833089076 44-45 0.0018437449769768256 46-47 0.0012606803261380004 48-49 0.003151700815345001 50-51 6.460986671457252E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.933741793759002E-4 60-61 0.0015285748954423253 62-63 0.0013552313505983504 64-65 8.667177242198752E-4 66-67 0.002789255221580326 68-69 0.0026001531726596258 70-71 4.7275512230175014E-5 72-73 0.0 74-75 0.0 76-77 0.09236059239368526 78-79 0.003151700815345001 80-81 3.151700815345001E-4 82-83 0.0 84-85 2.0486055299742507E-4 86-87 0.0924551434181456 88-89 0.4356596037051395 90-91 0.3880216458811998 92-93 0.0 94-95 0.0011030952853707504 96-97 1.5758504076725006E-5 98-99 0.0 100-101 5.830646508388251E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3172890.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.314575745191895 #Duplication Level Percentage of deduplicated Percentage of total 1 67.75290687516885 27.991826030510957 2 15.701939690257532 12.974379533591598 3 6.2957179228641245 7.803147449835962 4 3.1886302002379185 5.269476157245436 5 1.8929729831418884 3.91036878478087 6 1.2132112266952937 3.007398427213193 7 0.7775431698989365 2.248670633156235 8 0.5634139175303167 1.8621765577361256 9 0.40470332911386514 1.5048131710505497 >10 1.9660047252920045 14.23107039179011 >50 0.14580736336714548 4.1379372885408205 >100 0.0887545549882555 6.670049289465978 >500 0.004425949125164729 1.2070401500583936 >1k 0.0028234503037641214 2.3574264016892212 >5k 7.630946766930059E-4 2.233651663434031 >10k+ 3.815473383465029E-4 2.5905680699004123 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 30131 0.9496389726716021 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14411 0.4541916044993681 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14328 0.4515756928226317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12226 0.3853269416840798 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11074 0.34901934829130543 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9367 0.2952198153733663 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8588 0.2706680660218287 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7649 0.2410735953657391 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7488 0.23599935705303368 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7368 0.23221731607461965 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6796 0.21418958741084626 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6744 0.21255070298686687 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6427 0.2025598114022232 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5385 0.1697190889063283 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5037 0.1587511700689277 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4967 0.15654497949818622 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4066 0.12814815515192773 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3790 0.11944946090157553 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3620 0.11409156951548904 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3550 0.11188537894474754 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.151700815345001E-5 0.0 4 0.0 0.0 0.0 3.151700815345001E-5 0.0 5 0.0 0.0 0.0 3.151700815345001E-5 0.0 6 0.0 0.0 0.0 3.151700815345001E-5 0.0 7 0.0 0.0 0.0 3.151700815345001E-5 0.0 8 0.0 0.0 0.0 3.151700815345001E-5 0.0 9 0.0 0.0 0.0 9.455102446035003E-5 0.0 10-11 0.0 0.0 0.0 1.1030952853707503E-4 0.0 12-13 0.0 0.0 0.0 1.8910204892070006E-4 0.0 14-15 0.0 0.0 0.0 3.151700815345001E-4 0.0 16-17 0.0 0.0 0.0 4.885136263784751E-4 0.0 18-19 0.0 0.0 0.0 6.618571712224501E-4 0.0 20-21 0.0 0.0 0.0 8.194422119897002E-4 0.0 22-23 0.0 0.0 0.0 0.0013709898546750755 0.0 24-25 0.0 0.0 0.0 0.0018595034810535505 0.0 26-27 0.0 0.0 0.0 0.002820772229733776 0.0 28-29 0.0 0.0 0.0 0.005988231549155502 0.0 30-31 0.0 0.0 0.0 0.015506368011497403 0.0 32-33 0.0 0.0 0.0 0.03339227013858029 0.0 34-35 0.0 0.0 0.0 0.058921046742874794 0.0 36-37 0.0 0.0 0.0 0.0961111163639458 0.0 38-39 0.0 0.0 0.0 0.15317265962576704 0.0 40-41 0.0 0.0 0.0 0.21696308412834986 0.0 42-43 0.0 0.0 0.0 0.2836688318851268 0.0 44-45 0.0 0.0 0.0 0.36802410420783577 0.0 46-47 0.0 0.0 0.0 0.46879973777849215 0.0 48-49 0.0 0.0 0.0 0.5718288374321202 0.0 50-51 0.0 0.0 0.0 0.6877326349164327 0.0 52-53 0.0 0.0 0.0 0.8075287829076961 0.0 54-55 0.0 0.0 0.0 0.941113621966094 0.0 56-57 0.0 0.0 0.0 1.1096193060585144 0.0 58-59 0.0 1.5758504076725006E-5 0.0 1.2901329702573996 0.0 60-61 0.0 3.151700815345001E-5 0.0 1.4956868974342004 0.0 62-63 0.0 3.151700815345001E-5 0.0 1.7026906069860601 0.0 64-65 0.0 3.151700815345001E-5 0.0 1.917069296445827 0.0 66-67 0.0 3.151700815345001E-5 0.0 2.169331429706041 0.0 68-69 0.0 3.151700815345001E-5 0.0 2.4641572824774887 0.0 70-71 0.0 3.151700815345001E-5 0.0 2.78413370775539 0.0 72-73 0.0 3.151700815345001E-5 0.0 3.110618395216979 1.5758504076725006E-5 74-75 0.0 3.151700815345001E-5 0.0 3.4646174307965296 3.151700815345001E-5 76-77 0.0 3.151700815345001E-5 0.0 3.8253611061209183 3.151700815345001E-5 78-79 0.0 3.151700815345001E-5 0.0 4.266000397114302 3.151700815345001E-5 80-81 0.0 3.151700815345001E-5 0.0 4.746240808852497 3.151700815345001E-5 82-83 0.0 3.151700815345001E-5 0.0 5.215056305135066 3.151700815345001E-5 84-85 0.0 3.151700815345001E-5 0.0 5.713040792463653 3.151700815345001E-5 86-87 0.0 3.151700815345001E-5 0.0 6.235781889696775 3.151700815345001E-5 88-89 0.0 3.151700815345001E-5 0.0 6.795256059932743 3.151700815345001E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 960 0.0 27.18753 2 TTTGCGT 945 0.0 27.116915 3 GTAAGCG 575 0.0 25.997276 94-95 TTGCGTT 1055 0.0 24.289557 4 GAGTACT 12530 0.0 22.971292 12-13 GTACATG 14395 0.0 22.239738 1 TACATGG 14775 0.0 21.06952 2 GTACTTT 13635 0.0 20.987844 14-15 AGTACTT 13110 0.0 20.88713 12-13 ACATGGG 14730 0.0 20.360697 3 CATGGGG 5195 0.0 19.182802 4 ACTTTTT 15940 0.0 18.056395 16-17 ACCGTAT 135 0.00945586 17.575779 8 TACTTTT 15745 0.0 17.15044 14-15 AATTCGC 320 0.0 17.054668 78-79 ATTCGCG 310 0.0 16.852804 80-81 CGCGGAA 315 0.0 16.671942 82-83 CATGGGA 9425 0.0 16.665754 4 TAGACTG 1150 0.0 16.505947 5 GTATAGG 810 0.0 16.46816 1 >>END_MODULE