FastQCFastQC Report
Fri 27 May 2016
SRR938988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183858
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT266960.8384796055602982No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT158950.49923708909128484No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150080.47137780642227134No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT125250.3933906600105909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114660.3601291263617913No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC104040.32677336740520463No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT80630.2532462188954407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72700.22833932920375216No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT68070.21379722336863013No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC64670.20311835515277377No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC64550.20274145392162587No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG59170.18584371539182967No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC57650.1810696331306233No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA56450.17730062081914458No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA50050.15719922182459142No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA44490.13973613144807337No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA40430.12698430646090372No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT37770.11862966250379256No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA36350.11416966460187608No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC35920.11281910185692956No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC32060.10069544558833968No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG32050.10066403715241069No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG10150.023.7812352
GTACATG144400.022.8213081
TACATGG145300.022.1499942
TTTGCGT10900.022.1449153
GAGTACT117700.021.79464112-13
ACATGGG145350.020.9375693
AGTACTT123850.020.40667212-13
GTACTTT129500.019.84589214-15
GTATAGG8950.019.072271
TTGCGTT13100.018.0646274
CATGGGA94200.017.6856924
CATGGGG51250.017.4541324
TACTTTT151000.016.72237814-15
ACTTTTT157200.016.7117516-17
TATCACG2002.839143E-416.5652642
AGAGTAC257800.016.55031610-11
AGTACAT113200.016.0133672
CCTATAC7750.015.8782253
GTGTAGC16600.015.4244111
ATACCGT2601.2562935E-414.5619546
Produced by FastQC (version 0.11.5)