##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938988_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3183858 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78643771173212 33.0 31.0 34.0 30.0 34.0 2 31.84959348061377 33.0 31.0 34.0 30.0 34.0 3 31.892071191617212 34.0 31.0 34.0 30.0 34.0 4 35.42365771337792 37.0 35.0 37.0 33.0 37.0 5 35.46993239020082 37.0 35.0 37.0 33.0 37.0 6 35.557634165845336 37.0 35.0 37.0 33.0 37.0 7 35.47939072659648 37.0 35.0 37.0 33.0 37.0 8 35.46436430267933 37.0 35.0 37.0 33.0 37.0 9 37.058081421972965 39.0 37.0 39.0 33.0 39.0 10-11 36.950994045588715 39.0 37.0 39.0 33.0 39.0 12-13 36.82922463878728 39.0 37.0 39.0 33.0 39.0 14-15 37.998227779002704 40.0 38.0 41.0 33.0 41.0 16-17 37.94274210721709 40.0 38.0 41.0 33.0 41.0 18-19 37.918800084677144 40.0 38.0 41.0 33.0 41.0 20-21 37.80672347824557 40.0 38.0 41.0 32.0 41.0 22-23 37.88747174025977 40.0 38.0 41.0 32.5 41.0 24-25 37.8195937130362 40.0 38.0 41.0 32.0 41.0 26-27 37.72536667778525 40.0 37.5 41.0 32.0 41.0 28-29 37.605093725913655 40.0 37.0 41.0 32.0 41.0 30-31 37.43037189472646 40.0 37.0 41.0 31.5 41.0 32-33 37.22804848708705 40.0 36.5 41.0 31.0 41.0 34-35 36.89408557165552 40.0 36.0 41.0 30.5 41.0 36-37 36.62008591463564 39.5 36.0 41.0 30.0 41.0 38-39 36.4083432741033 39.0 35.0 41.0 30.0 41.0 40-41 36.283930847418446 39.0 35.0 41.0 30.0 41.0 42-43 36.06939301313061 39.0 35.0 41.0 29.5 41.0 44-45 35.962155661464806 39.0 35.0 41.0 28.5 41.0 46-47 35.69609338733072 39.0 35.0 41.0 27.0 41.0 48-49 35.4841900926486 39.0 35.0 41.0 26.0 41.0 50-51 34.30694412250798 38.0 34.0 40.0 24.0 40.5 52-53 34.47387006581324 38.0 34.5 39.5 24.0 40.5 54-55 34.98542868431946 39.0 35.0 40.5 24.5 41.0 56-57 34.878243941783836 39.0 35.0 41.0 24.0 41.0 58-59 34.69220832084848 38.5 34.0 40.5 24.0 41.0 60-61 34.400997783192594 38.0 34.0 40.0 23.0 41.0 62-63 34.07306560154379 38.0 34.0 40.0 22.5 41.0 64-65 33.676156568540435 37.0 33.5 40.0 22.0 41.0 66-67 33.274961226285846 37.0 33.0 39.5 20.5 41.0 68-69 32.873666947458084 36.0 32.5 39.0 19.5 41.0 70-71 32.42713713991013 35.5 32.0 39.0 18.0 40.0 72-73 31.98310665865123 35.0 32.0 38.0 15.5 40.0 74-75 31.521439712449485 35.0 31.0 37.0 12.0 39.5 76-77 31.111754512921117 35.0 31.0 37.0 8.0 39.0 78-79 30.670955017466234 35.0 31.0 36.0 7.0 39.0 80-81 30.207987290890486 34.5 30.0 36.0 2.0 37.5 82-83 29.818204674957236 34.0 30.0 35.0 2.0 37.0 84-85 29.45220923797481 34.0 29.5 35.0 2.0 37.0 86-87 29.088892312408404 34.0 29.0 35.0 2.0 36.0 88-89 28.656207971586674 34.0 29.0 35.0 2.0 36.0 90-91 28.20847223714123 34.0 29.0 35.0 2.0 35.5 92-93 27.78915548369305 34.0 27.5 35.0 2.0 35.0 94-95 27.31528808759687 34.0 26.5 35.0 2.0 35.0 96-97 26.720978919285972 33.5 25.0 35.0 2.0 35.0 98-99 26.02127120619073 33.0 24.5 35.0 2.0 35.0 100-101 23.36357447474102 30.0 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.37722647087889527 1101 2 0.39709932142197957 1101 3 0.3980708680351377 1101 4 0.2789297712009713 1101 5 0.16128424824190546 1101 6 0.23007011369393382 1101 7 0.29733563574872335 1101 8 0.36524594050615633 1101 9 0.4429954446579316 1101 10-11 0.4960008238171767 1101 12-13 0.5133567017502614 1101 14-15 0.2796132904875108 1101 16-17 0.4485568775618418 1101 18-19 0.5447943867907341 1101 20-21 0.6392453964499722 1101 22-23 0.5896051490254521 1101 24-25 0.6363680492025097 1101 26-27 0.6346687184423487 1101 28-29 0.5979001180376144 1101 30-31 0.5705932005706487 1101 32-33 0.5600570591090985 1101 34-35 0.47353924912494705 1101 36-37 0.47921401493628224 1101 38-39 0.5288116020982727 1101 40-41 0.4176437975494025 1101 42-43 0.40858695472684303 1101 44-45 0.572636793798253 1101 46-47 0.5689691712896092 1101 48-49 0.6816932728944067 1101 50-51 0.7177551840043961 1101 52-53 0.6414045507129416 1101 54-55 0.5620890897678734 1101 56-57 0.6209609439314221 1101 58-59 0.56542107591536 1101 60-61 0.5009997797413419 1101 62-63 0.5216197280202763 1101 64-65 0.5962061162734571 1101 66-67 0.5944095321280756 1101 68-69 0.48400452304137076 1101 70-71 0.4593842321593513 1101 72-73 0.29764541896432917 1101 74-75 0.33045940803429374 1101 76-77 0.3013235136883523 1101 78-79 0.27653065345849015 1101 80-81 0.19505025567364598 1101 82-83 0.029976155741731958 1101 84-85 -0.09611399593995529 1101 86-87 -0.09869866792471171 1101 88-89 -0.1590922400561965 1101 90-91 -0.045087553705606354 1101 92-93 -0.0528262215494415 1101 94-95 -0.08619877817074695 1101 96-97 -0.11144761911302226 1101 98-99 -0.13288094990970833 1101 100-101 0.22285267784036833 1102 1 0.24037766846717545 1102 2 0.15879788775630388 1102 3 0.15087711256323288 1102 4 0.1413621511830243 1102 5 0.2539041007955305 1102 6 0.27782357436129956 1102 7 0.21995352558516146 1102 8 0.12899587710377602 1102 9 0.2565862688447851 1102 10-11 0.1914541012241031 1102 12-13 0.2618895155148664 1102 14-15 0.3511412658234363 1102 16-17 0.328027737827405 1102 18-19 0.4172659549576707 1102 20-21 0.4558241819029263 1102 22-23 0.35254309408261264 1102 24-25 0.43432505908005936 1102 26-27 0.47009548814063606 1102 28-29 0.504600261161599 1102 30-31 0.4539109865374442 1102 32-33 0.46559539494544566 1102 34-35 0.39455454729256445 1102 36-37 0.501072289112436 1102 38-39 0.45219158855773145 1102 40-41 0.4739565796900038 1102 42-43 0.337679771402442 1102 44-45 0.4869488614215811 1102 46-47 0.5071730755531192 1102 48-49 0.5035711742425093 1102 50-51 0.4608855084261094 1102 52-53 0.4438916331669134 1102 54-55 0.4550023023830789 1102 56-57 0.3328236397345208 1102 58-59 0.37597878531413187 1102 60-61 0.3603072493137134 1102 62-63 0.2607824416863025 1102 64-65 0.2727887499752981 1102 66-67 0.28840433423546585 1102 68-69 0.17952969323541623 1102 70-71 0.25164561316245226 1102 72-73 0.31610516010618994 1102 74-75 0.29156717444007185 1102 76-77 0.19257431586630247 1102 78-79 0.22225788428506021 1102 80-81 0.21931462265324697 1102 82-83 0.13319782369849875 1102 84-85 0.15025585937030073 1102 86-87 0.19225750614404546 1102 88-89 0.2956281499290938 1102 90-91 0.20315140380487406 1102 92-93 0.21616334296992434 1102 94-95 0.1852286354752124 1102 96-97 0.027981549951906715 1102 98-99 0.09279039860932414 1102 100-101 0.3090877452012677 1103 1 -0.07252747887505606 1103 2 0.09856452288717676 1103 3 0.20401492397919085 1103 4 0.2579692502404498 1103 5 0.2618318487895053 1103 6 0.16293123405505128 1103 7 0.28446656037964857 1103 8 0.17580112606134435 1103 9 0.2773000289625216 1103 10-11 0.24974823381458577 1103 12-13 0.338937371080938 1103 14-15 0.29274945987368284 1103 16-17 0.3724119576669267 1103 18-19 0.3853473523437003 1103 20-21 0.35818849039306144 1103 22-23 0.38952976145007057 1103 24-25 0.39125899159345323 1103 26-27 0.29797531924894827 1103 28-29 0.2796893935768878 1103 30-31 0.2974161461435898 1103 32-33 0.3458927511947891 1103 34-35 0.21239876923446843 1103 36-37 0.17346883419110526 1103 38-39 0.36268490472157566 1103 40-41 0.4145092006648099 1103 42-43 0.2449658536057413 1103 44-45 0.3055416204530772 1103 46-47 0.31700238807282943 1103 48-49 0.22373918994033204 1103 50-51 0.1184968034960221 1103 52-53 0.22632772938612078 1103 54-55 0.32735044877923514 1103 56-57 0.40569935366983145 1103 58-59 0.24831225880654273 1103 60-61 0.39079863829019956 1103 62-63 0.3424297050802565 1103 64-65 0.1289222427395842 1103 66-67 0.08818774465629531 1103 68-69 0.15987610516295092 1103 70-71 0.10402547055058875 1103 72-73 0.22122184254074995 1103 74-75 0.16012511269999763 1103 76-77 0.12381467992951656 1103 78-79 0.3347710241988615 1103 80-81 0.30565261627601004 1103 82-83 0.3066416199071931 1103 84-85 0.25950723468127634 1103 86-87 0.26456075783471533 1103 88-89 0.2863708616569056 1103 90-91 0.12365689628884624 1103 92-93 0.12628633256311161 1103 94-95 0.14200357503160532 1103 96-97 0.044104005188604134 1103 98-99 0.01601760523884721 1103 100-101 0.3380395742772677 1104 1 0.1642842975406822 1104 2 0.11082289158118996 1104 3 0.235438780650572 1104 4 0.2601904247388518 1104 5 0.1685687129521085 1104 6 0.21723410481831706 1104 7 0.23636228566646622 1104 8 0.27383009562496596 1104 9 0.2653638353431589 1104 10-11 0.23294045184591283 1104 12-13 0.3396887855080948 1104 14-15 0.32079735394879805 1104 16-17 0.31211839854910295 1104 18-19 0.454624160976941 1104 20-21 0.437465822888214 1104 22-23 0.40703788130967666 1104 24-25 0.4580612075293331 1104 26-27 0.40739632087291966 1104 28-29 0.3448391526004144 1104 30-31 0.4235971273369543 1104 32-33 0.5124759273695503 1104 34-35 0.42612296903539715 1104 36-37 0.3551375963054042 1104 38-39 0.38744883944838193 1104 40-41 0.38099546238622395 1104 42-43 0.412707092015296 1104 44-45 0.4132892106882906 1104 46-47 0.3488594130610423 1104 48-49 0.4090068833763354 1104 50-51 0.29868794761042494 1104 52-53 0.24907872472375203 1104 54-55 0.38683464124610367 1104 56-57 0.3908072692232949 1104 58-59 0.46957319442391565 1104 60-61 0.39966860266758886 1104 62-63 0.290651521309492 1104 64-65 0.22885119396804043 1104 66-67 0.31641178729865516 1104 68-69 0.2407240411470255 1104 70-71 0.3325834751605754 1104 72-73 0.2759837473026536 1104 74-75 0.12219358145163639 1104 76-77 0.12819383808342621 1104 78-79 0.13037647760134163 1104 80-81 -0.023023584677421383 1104 82-83 -0.0014605593583496557 1104 84-85 0.10518668357854821 1104 86-87 0.14526495920723193 1104 88-89 0.2116396682994761 1104 90-91 0.21050174148765066 1104 92-93 0.252229284001114 1104 94-95 0.4203667684938104 1104 96-97 0.2916402171431294 1104 98-99 0.27194804947374607 1104 100-101 0.6135847180773624 1105 1 0.0012347025519510169 1105 2 0.035982361084489156 1105 3 0.05390047742109516 1105 4 0.08156775246838066 1105 5 0.03771679047503085 1105 6 0.18504736552080914 1105 7 0.13909591076654948 1105 8 0.16494953884567565 1105 9 0.1984234095695001 1105 10-11 0.16219183752361488 1105 12-13 0.21675068786591822 1105 14-15 0.3064000795347752 1105 16-17 0.23370774488224555 1105 18-19 0.23670494456683144 1105 20-21 0.24993188436932456 1105 22-23 0.20801672554031114 1105 24-25 0.2772387082609029 1105 26-27 0.2355638177135475 1105 28-29 0.21366185981429453 1105 30-31 0.2901659846124147 1105 32-33 0.18905537485635904 1105 34-35 0.12019555914598357 1105 36-37 0.15780353194736563 1105 38-39 0.24787001472602554 1105 40-41 0.1932758736036959 1105 42-43 0.11681696224233207 1105 44-45 0.2597278233706035 1105 46-47 0.37421648433166155 1105 48-49 0.257508872256615 1105 50-51 0.3082691411958436 1105 52-53 0.2112770296468014 1105 54-55 0.23568327732763805 1105 56-57 0.20028818808491167 1105 58-59 0.1855556776998526 1105 60-61 0.2644686373966181 1105 62-63 0.16462119986228174 1105 64-65 0.25548712972790355 1105 66-67 0.1304924272561223 1105 68-69 0.07904945861894674 1105 70-71 -0.017053263440210742 1105 72-73 0.11797248823585704 1105 74-75 0.18801351355851992 1105 76-77 0.18670234401683672 1105 78-79 0.2440299182945509 1105 80-81 0.09598557671289853 1105 82-83 0.12975907283546206 1105 84-85 0.10666974192011125 1105 86-87 0.24211597915820704 1105 88-89 0.1496677452221391 1105 90-91 0.19668891168242197 1105 92-93 0.13602395609579787 1105 94-95 0.05900726619087138 1105 96-97 0.019253389308023117 1105 98-99 0.22408345625898463 1105 100-101 0.6015503577722185 1106 1 0.2328985133390873 1106 2 0.25481791262576436 1106 3 0.3291640724768605 1106 4 0.15029910042907346 1106 5 0.16275506292456043 1106 6 0.23177531813261965 1106 7 0.25002455654698963 1106 8 0.2625437831342978 1106 9 0.294971866110437 1106 10-11 0.2106468459802855 1106 12-13 0.27239341295098285 1106 14-15 0.3541048581453694 1106 16-17 0.4274458701182624 1106 18-19 0.31893710056898783 1106 20-21 0.4697952566125849 1106 22-23 0.37474673485041166 1106 24-25 0.415997303774823 1106 26-27 0.31417751640217517 1106 28-29 0.33998794668580956 1106 30-31 0.45145076366056003 1106 32-33 0.5790039436596288 1106 34-35 0.48965942355947334 1106 36-37 0.3309078791586231 1106 38-39 0.3398896660237156 1106 40-41 0.47775983944221423 1106 42-43 0.581175945083487 1106 44-45 0.48336918972427156 1106 46-47 0.3421034022826106 1106 48-49 0.3552444749194166 1106 50-51 0.20760512146253518 1106 52-53 0.1600157800303208 1106 54-55 0.09859017410494886 1106 56-57 0.23116698693928583 1106 58-59 0.36671507424808425 1106 60-61 0.36074785247346597 1106 62-63 0.37494370093423157 1106 64-65 0.2831391528648126 1106 66-67 0.4190492052871164 1106 68-69 0.43327848370485356 1106 70-71 0.5289092401809441 1106 72-73 0.38626143468740537 1106 74-75 0.2884365106583182 1106 76-77 0.3139748714422126 1106 78-79 0.41349657288454367 1106 80-81 0.4364121587667604 1106 82-83 0.5597910636887562 1106 84-85 0.5429451226180255 1106 86-87 0.5969261324836879 1106 88-89 0.6292041256889149 1106 90-91 0.6055071060006689 1106 92-93 0.6555329970603445 1106 94-95 0.7617607793560097 1106 96-97 0.8612046337214636 1106 98-99 0.6799178685838747 1106 100-101 1.1619731625189438 1107 1 0.032037622168918034 1107 2 0.013859321984796225 1107 3 0.10411247900632148 1107 4 0.1494020219549128 1107 5 0.0677041194505037 1107 6 0.05941916839510242 1107 7 -0.014914320867426056 1107 8 0.07618406341611461 1107 9 0.05671388584116244 1107 10-11 0.15970566357835736 1107 12-13 0.1035382338332127 1107 14-15 0.07025222901376793 1107 16-17 0.048951201547708934 1107 18-19 0.13171275011754346 1107 20-21 0.19301732874302502 1107 22-23 0.17329845102862151 1107 24-25 0.15742797519201446 1107 26-27 0.24657559091260595 1107 28-29 0.1963761724995834 1107 30-31 0.3057834797750161 1107 32-33 0.21223410741291104 1107 34-35 0.059786335372031374 1107 36-37 0.15504346717418827 1107 38-39 0.15485691828443038 1107 40-41 0.15929885610587036 1107 42-43 0.23555458803250673 1107 44-45 0.2579274421170652 1107 46-47 0.25445856452548554 1107 48-49 0.07319027354633789 1107 50-51 0.13759180278467653 1107 52-53 0.10434477949824128 1107 54-55 -0.004664579098466959 1107 56-57 0.2085535156789291 1107 58-59 0.25285043059823664 1107 60-61 -0.0758787407991548 1107 62-63 -0.06030128523876499 1107 64-65 0.04444039373309039 1107 66-67 0.09239628715709358 1107 68-69 0.012678314039099803 1107 70-71 0.09992151178114028 1107 72-73 0.1102453776910508 1107 74-75 0.020920355537494828 1107 76-77 0.097613491316352 1107 78-79 0.17335683197480733 1107 80-81 0.22707339685171846 1107 82-83 0.24342350651302525 1107 84-85 0.27566563288192825 1107 86-87 0.2986182170682312 1107 88-89 0.40499273600060803 1107 90-91 0.3743498164359913 1107 92-93 0.2937913627441979 1107 94-95 0.3864043229534815 1107 96-97 0.4166217293788641 1107 98-99 0.517987547789776 1107 100-101 0.7619714229450345 1108 1 0.14190984502781845 1108 2 0.17485311945909743 1108 3 0.07563820303969138 1108 4 0.1870244668037202 1108 5 0.12091326485739984 1108 6 0.18901468918712538 1108 7 0.2258709338912368 1108 8 0.16122062476159016 1108 9 0.24651731450305192 1108 10-11 0.2296225894737134 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19643.0 15 18982.0 16 24122.0 17 32617.0 18 27941.0 19 19466.0 20 19164.0 21 20400.0 22 21780.0 23 24302.0 24 27604.0 25 31662.0 26 37004.0 27 43123.0 28 50743.0 29 60649.0 30 72595.0 31 88384.0 32 110085.0 33 140530.0 34 188350.0 35 268045.0 36 405766.0 37 632391.0 38 608944.0 39 99671.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.72765241712968 19.546591324287206 14.801312822063476 21.924443436519635 2 14.6877435218282 21.452616840967654 40.38789972158156 23.47173991562258 3 20.014988709741814 25.585677231590342 30.523872380944596 23.87546167772325 4 12.260562297583277 17.147740274014982 39.0412085156741 31.55048891272764 5 12.863999678044724 38.327769334137386 34.59953354214313 14.208697445674764 6 29.804984508085752 37.16450033429341 17.841901062352086 15.188614095268752 7 26.726557460330234 34.070226707726974 21.69583138950749 17.5073844424353 8 25.097724492846424 34.09765124859445 20.481356194341835 20.323268064217288 9 25.573982819732944 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29.904520821359267 21.511678725627924 34-35 22.349992031355 26.807404550056884 29.612950853086407 21.22965256550171 36-37 21.939220050887947 26.450249669392612 30.32112039416362 21.28940988555582 38-39 21.53298954614026 27.186763245472395 30.14472606945487 21.13552113893248 40-41 21.492845095984695 27.37817813083194 29.853665513822058 21.275311259361303 42-43 21.16099900862174 27.56061572201488 29.04049807046018 22.2378871989032 44-45 21.635496865645425 26.933178416231673 29.10827264904453 22.32305206907837 46-47 22.297974585467305 27.502345666514366 28.851737609424294 21.34794213859403 48-49 22.085448818206075 27.361269712691076 28.7667346496795 21.786546819423354 50-51 21.12273833872925 27.743471392686896 29.455343774724486 21.67844649385936 52-53 21.838449896924217 28.27836827031871 28.14773663715002 21.73544519560705 54-55 21.17133821963464 27.378676137284362 28.76875441645047 22.681231226630523 56-57 22.080204838514085 27.51846464590323 28.210059900359756 22.191270615222926 58-59 22.769518474840595 27.21244223281755 28.144785491893217 21.873253800448637 60-61 21.948357715119506 27.384844528534018 29.32726533303433 21.339532423312143 62-63 21.635425668470816 27.475511934181334 29.13566571658151 21.753396680766336 64-65 21.267948162181497 28.19796723358488 28.666742707937065 21.86734189629656 66-67 21.62816841323574 27.468626932055635 28.122087350837205 22.78111730387142 68-69 21.22755011865353 27.578085461826017 28.703362384579638 22.491002034940813 70-71 22.092481654904276 27.45378097620999 28.217683338778983 22.23605403010675 72-73 21.77376368248885 26.911488282579278 29.084098312017776 22.230649722914098 74-75 21.150053721117494 28.41321831753454 28.2624263556229 22.174301605725073 76-77 21.53776849157592 27.321324943318753 28.303915736774087 22.836990828331242 78-79 21.6244303962181 27.393615005324932 28.56911171498271 22.41284288347426 80-81 21.984062805111158 27.522145651806255 28.23030751493865 22.26348402814393 82-83 21.7166200011592 27.17578158868455 28.902897578199934 22.20470083195632 84-85 21.486312725673752 27.476628487681637 28.509430657340122 22.527628129304496 86-87 21.533105744696847 27.54904853083407 28.511399120515634 22.406446603953448 88-89 21.52305265708373 27.360935216140476 28.09849893715387 23.017513189621923 90-91 21.57511735420473 26.8292366980796 28.510401338347396 23.085244609368274 92-93 22.100962092174868 27.397485134627658 28.404097987832806 22.097454785364672 94-95 21.699335955470847 26.97579788981967 28.477212807692503 22.847653347016983 96-97 21.00925870779359 26.923254953883397 29.148096828197072 22.91938951012594 98-99 21.50454410806327 27.284286193889844 28.036574046645736 23.17459565140115 100-101 21.521223630484744 26.276395971838824 28.909165777277885 23.293214620398548 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4196.0 1 2485.0 2 865.5 3 932.5 4 803.5 5 829.5 6 1412.5 7 2806.5 8 4606.5 9 5444.5 10 4948.0 11 4457.0 12 4679.0 13 5151.0 14 5470.5 15 5732.0 16 6202.5 17 6439.5 18 6418.5 19 6746.5 20 7313.0 21 8189.5 22 9444.0 23 11245.5 24 13563.5 25 16304.5 26 20490.0 27 24909.5 28 27842.0 29 32094.0 30 38503.5 31 45067.0 32 51725.5 33 59942.5 34 67953.0 35 73569.0 36 81457.5 37 89766.0 38 93188.0 39 94562.0 40 96182.5 41 98744.5 42 100956.0 43 104269.5 44 107921.0 45 110297.0 46 113527.0 47 133602.0 48 184903.5 49 182780.0 50 130099.0 51 106914.5 52 96993.0 53 91060.5 54 86006.0 55 78643.5 56 70952.0 57 62716.0 58 55025.5 59 47725.5 60 40711.5 61 33615.0 62 26907.5 63 20954.5 64 15499.0 65 11487.0 66 8242.5 67 6255.0 68 4562.0 69 2861.5 70 2048.5 71 1568.0 72 1142.0 73 845.0 74 609.5 75 503.0 76 389.0 77 220.5 78 114.0 79 76.0 80 55.0 81 39.0 82 33.0 83 26.0 84 23.0 85 18.0 86 13.5 87 8.5 88 10.0 89 9.0 90 5.5 91 6.0 92 5.5 93 5.5 94 4.0 95 2.5 96 2.0 97 1.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2832412752076255 2 0.08401756611004636 3 0.08784939529338306 4 0.10141783961470643 5 0.10339657107823275 6 0.07918066697698202 7 0.08938840865390353 8 0.08546235416277986 9 0.09055052078327613 10-11 0.08404897454597535 12-13 0.08828911339638891 14-15 0.09761741886729873 16-17 0.08574503008614077 18-19 0.09680079953314501 20-21 0.09888946052242278 22-23 0.11182973612516639 24-25 0.12356078694464388 26-27 0.1266859263195783 28-29 0.11870818359361504 30-31 0.11156276441976999 32-33 0.10597206282440988 34-35 0.3785187655982145 36-37 0.5801766284802903 38-39 0.5636557911816419 40-41 0.526546724131541 42-43 0.5515007264771231 44-45 0.5745859268849302 46-47 0.5626350170139498 48-49 0.5844010631127393 50-51 0.5762662782071312 52-53 0.5800666989545388 54-55 0.5696548024440788 56-57 0.5422980547499292 58-59 0.5389530563234918 60-61 0.4881970238622451 62-63 0.5598867788701632 64-65 0.5939335234171876 66-67 0.6068266863660376 68-69 0.6090880937529249 70-71 0.6046752085049019 72-73 0.5953469030339921 74-75 0.5936665517117912 76-77 0.5763133908610246 78-79 0.5534794579406493 80-81 0.5493806570519163 82-83 0.561786989243867 84-85 0.5715864212537116 86-87 0.5625879043600562 88-89 0.5748057859364331 90-91 0.5892379622458037 92-93 0.5979066905622047 94-95 0.5970743670100865 96-97 0.5933524673525012 98-99 0.5981893664855656 100-101 0.5746016311028947 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3183858.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.26830458121698 #Duplication Level Percentage of deduplicated Percentage of total 1 69.8114308143626 30.206222517263974 2 14.742101747556188 12.757314971611047 3 5.965448436455508 7.743445197363045 4 3.028513432429947 5.241545664907434 5 1.728438664683114 3.739330529673047 6 1.1114313334190173 2.8853849673289296 7 0.7204608294207973 2.1821183024350685 8 0.5118962498304249 1.771910628131645 9 0.36715136566284945 1.4297415405218945 >10 1.7914245313775474 13.630456844788036 >50 0.13510852331077497 4.057222094951819 >100 0.07893922220520525 6.256912421108604 >500 0.0043012962655182155 1.2573451702964973 >1k 0.002260003122413834 2.128749092150851 >5k 6.561299387653066E-4 1.8104382513878037 >10k+ 4.3741995917687105E-4 2.9018618060802455 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26696 0.8384796055602982 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15895 0.49923708909128484 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15008 0.47137780642227134 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 12525 0.3933906600105909 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11466 0.3601291263617913 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10404 0.32677336740520463 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8063 0.2532462188954407 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7270 0.22833932920375216 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6807 0.21379722336863013 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6467 0.20311835515277377 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6455 0.20274145392162587 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5917 0.18584371539182967 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5765 0.1810696331306233 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5645 0.17730062081914458 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5005 0.15719922182459142 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4449 0.13973613144807337 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4043 0.12698430646090372 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3777 0.11862966250379256 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3635 0.11416966460187608 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3592 0.11281910185692956 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3206 0.10069544558833968 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3205 0.10066403715241069 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.28168718579786E-5 0.0 3 0.0 0.0 0.0 6.28168718579786E-5 0.0 4 0.0 0.0 0.0 9.422530778696788E-5 0.0 5 0.0 0.0 0.0 9.422530778696788E-5 0.0 6 0.0 0.0 0.0 9.422530778696788E-5 0.0 7 0.0 0.0 0.0 9.422530778696788E-5 0.0 8 0.0 0.0 0.0 9.422530778696788E-5 0.0 9 0.0 0.0 0.0 9.422530778696788E-5 0.0 10-11 1.570421796449465E-5 0.0 0.0 9.422530778696788E-5 0.0 12-13 3.14084359289893E-5 0.0 0.0 1.5704217964494647E-4 0.0 14-15 3.14084359289893E-5 0.0 0.0 2.041548335384304E-4 0.0 16-17 3.14084359289893E-5 0.0 0.0 4.240138850413555E-4 0.0 18-19 3.14084359289893E-5 0.0 0.0 5.496476287573127E-4 0.0 20-21 3.14084359289893E-5 0.0 0.0 7.223940263667537E-4 0.0 22-23 3.14084359289893E-5 0.0 0.0 0.0010364783856566468 0.0 24-25 3.14084359289893E-5 0.0 0.0 0.0014290838347690129 0.0 26-27 3.14084359289893E-5 0.0 0.0 0.002182886297064756 0.0 28-29 3.14084359289893E-5 0.0 0.0 0.005166687710318739 0.0 30-31 3.14084359289893E-5 0.0 0.0 0.014793373322553958 0.0 32-33 3.14084359289893E-5 0.0 0.0 0.033104491469154715 0.0 34-35 3.14084359289893E-5 1.570421796449465E-5 0.0 0.05564004424820453 0.0 36-37 3.14084359289893E-5 3.14084359289893E-5 0.0 0.09301608300370179 0.0 38-39 3.14084359289893E-5 3.14084359289893E-5 0.0 0.14977112672738546 0.0 40-41 3.14084359289893E-5 3.14084359289893E-5 0.0 0.21539905360100858 0.0 42-43 3.14084359289893E-5 3.14084359289893E-5 0.0 0.2836181764387733 0.0 44-45 3.14084359289893E-5 3.14084359289893E-5 0.0 0.36531151829007447 0.0 46-47 3.14084359289893E-5 3.14084359289893E-5 0.0 0.4674360477131832 0.0 48-49 3.14084359289893E-5 3.14084359289893E-5 0.0 0.5750256449879361 0.0 50-51 3.14084359289893E-5 3.14084359289893E-5 0.0 0.695445588339681 0.0 52-53 3.14084359289893E-5 3.14084359289893E-5 0.0 0.8130230682398525 0.0 54-55 3.14084359289893E-5 3.14084359289893E-5 0.0 0.9492257506459145 0.0 56-57 3.14084359289893E-5 3.14084359289893E-5 0.0 1.1225375001020774 0.0 58-59 3.14084359289893E-5 3.14084359289893E-5 0.0 1.3060883996710908 0.0 60-61 3.14084359289893E-5 3.14084359289893E-5 0.0 1.5127559080838404 0.0 62-63 3.14084359289893E-5 3.14084359289893E-5 0.0 1.726443201926719 0.0 64-65 3.14084359289893E-5 3.14084359289893E-5 0.0 1.945250070826023 0.0 66-67 3.14084359289893E-5 3.14084359289893E-5 0.0 2.197648261951381 0.0 68-69 3.14084359289893E-5 3.14084359289893E-5 0.0 2.4912700252335376 0.0 70-71 3.14084359289893E-5 3.14084359289893E-5 0.0 2.8126568458769206 0.0 72-73 3.14084359289893E-5 3.14084359289893E-5 0.0 3.1396971849875213 0.0 74-75 3.14084359289893E-5 3.14084359289893E-5 0.0 3.489838428723894 0.0 76-77 3.14084359289893E-5 3.14084359289893E-5 0.0 3.8536580463073418 0.0 78-79 3.14084359289893E-5 3.14084359289893E-5 0.0 4.296281429636624 0.0 80-81 3.14084359289893E-5 3.14084359289893E-5 0.0 4.775966767362112 0.0 82-83 3.14084359289893E-5 3.14084359289893E-5 0.0 5.2527154163282415 0.0 84-85 3.14084359289893E-5 3.14084359289893E-5 0.0 5.752078139163242 0.0 86-87 3.14084359289893E-5 3.14084359289893E-5 0.0 6.272029091749695 0.0 88-89 3.14084359289893E-5 3.14084359289893E-5 0.0 6.842814597887218 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 1015 0.0 23.781235 2 GTACATG 14440 0.0 22.821308 1 TACATGG 14530 0.0 22.149994 2 TTTGCGT 1090 0.0 22.144915 3 GAGTACT 11770 0.0 21.794641 12-13 ACATGGG 14535 0.0 20.937569 3 AGTACTT 12385 0.0 20.406672 12-13 GTACTTT 12950 0.0 19.845892 14-15 GTATAGG 895 0.0 19.07227 1 TTGCGTT 1310 0.0 18.064627 4 CATGGGA 9420 0.0 17.685692 4 CATGGGG 5125 0.0 17.454132 4 TACTTTT 15100 0.0 16.722378 14-15 ACTTTTT 15720 0.0 16.71175 16-17 TATCACG 200 2.839143E-4 16.565264 2 AGAGTAC 25780 0.0 16.550316 10-11 AGTACAT 11320 0.0 16.013367 2 CCTATAC 775 0.0 15.878225 3 GTGTAGC 1660 0.0 15.424411 1 ATACCGT 260 1.2562935E-4 14.561954 6 >>END_MODULE