FastQCFastQC Report
Fri 27 May 2016
SRR938988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3183858
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT300270.9431011056397616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144510.4538833076098243No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT141360.4439896502921927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122120.3835598195648173No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT109700.34455054214101255No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC95300.29932239440326797No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT88650.2784357845104901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77710.2440749556041758No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC76380.23989763362562022No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC73020.22934439915347984No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG68120.2139542655482751No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT65940.20710722651575542No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA64880.20377793230728253No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC52810.16586795014099248No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA50560.15880105205696987No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA49900.1567280952856566No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA40580.12745543299983855No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC37140.11665093104026623No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT36930.11599135388575747No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA35090.11021220167482344No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC31890.10016150217754685No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGCG8750.022.7767622
GTACATG142100.022.7657931
GAGTACT122900.022.27862212-13
TACATGG145450.021.5318282
AGTACTT128150.021.05116812-13
ACATGGG146350.020.6861023
TTTGCGT9900.020.6102853
GTACTTT133050.020.38288914-15
TTGCGTT10850.019.6803824
CATGGGG51500.019.5334684
ACTTTTT155450.017.53706216-17
TACACCG2453.8027174E-617.4311945
TACTTTT155950.017.1159714-15
CATGGGA93350.016.8253614
AGAGTAC258850.016.67306510-11
GTATAGT11500.016.1574881
GTAAGCG3550.016.03997294-95
GTACACG5103.6379788E-1215.881291
AGTACAT112650.015.4591482
GTGTAGC15750.015.1250391
Produced by FastQC (version 0.11.5)