##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938988_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3183858 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14192844027592 33.0 31.0 34.0 30.0 34.0 2 32.36225673381162 34.0 31.0 34.0 30.0 34.0 3 32.45813475349717 34.0 31.0 34.0 30.0 34.0 4 36.024796960165936 37.0 35.0 37.0 35.0 37.0 5 35.94661979271689 37.0 35.0 37.0 35.0 37.0 6 36.0048142850592 37.0 35.0 37.0 35.0 37.0 7 35.9517726607154 37.0 35.0 37.0 35.0 37.0 8 35.923260396663416 37.0 35.0 37.0 35.0 37.0 9 37.59089915442209 39.0 37.0 39.0 35.0 39.0 10-11 37.5114747579823 39.0 37.0 39.0 35.0 39.0 12-13 37.443328502715886 39.0 37.0 39.0 34.0 39.0 14-15 38.65645641231487 40.0 38.0 41.0 34.0 41.0 16-17 38.66917871337227 40.0 38.0 41.0 34.0 41.0 18-19 38.805646482977565 40.0 38.0 41.0 34.0 41.0 20-21 38.81102187974464 40.0 38.0 41.0 34.0 41.0 22-23 38.74921039192075 40.0 38.0 41.0 34.0 41.0 24-25 38.6803615299426 40.0 38.0 41.0 34.0 41.0 26-27 38.5183728357232 40.0 38.0 41.0 34.0 41.0 28-29 38.348181514376584 40.0 38.0 41.0 34.0 41.0 30-31 38.186548834778435 40.0 38.0 41.0 33.5 41.0 32-33 38.01205094573942 40.0 37.0 41.0 33.0 41.0 34-35 37.95538604422684 40.0 37.0 41.0 33.0 41.0 36-37 37.84084528895447 40.0 37.0 41.0 33.0 41.0 38-39 37.711702280692165 40.0 37.0 41.0 33.0 41.0 40-41 37.5767378758726 40.0 37.0 41.0 33.0 41.0 42-43 37.3926615131705 40.0 37.0 41.0 32.0 41.0 44-45 37.1228112246212 40.0 36.5 41.0 31.0 41.0 46-47 36.78537751997733 40.0 36.0 41.0 30.5 41.0 48-49 36.47132708179825 39.5 36.0 41.0 30.0 41.0 50-51 36.20507981197654 39.0 35.5 41.0 29.5 41.0 52-53 35.99619581024028 39.0 35.0 41.0 29.0 41.0 54-55 35.76741048124634 39.0 35.0 40.0 28.0 41.0 56-57 35.48259124621764 38.5 35.0 40.0 28.0 41.0 58-59 35.19221868563234 38.0 34.5 40.0 27.5 41.0 60-61 34.76688140614311 38.0 34.0 40.0 26.0 41.0 62-63 34.483007250951516 37.5 34.0 40.0 26.0 41.0 64-65 34.240455918574256 37.0 34.0 40.0 26.0 41.0 66-67 33.77584333220891 36.5 33.0 39.0 25.5 41.0 68-69 33.35119012845422 36.0 33.0 39.0 24.5 40.0 70-71 32.86872263147414 35.5 32.0 38.5 23.5 40.0 72-73 32.346329515952036 35.0 32.0 37.5 22.0 40.0 74-75 31.88142027690933 35.0 31.0 37.0 20.5 39.0 76-77 29.89462595379568 33.0 29.0 35.0 18.0 37.5 78-79 30.87444744709092 34.0 30.5 36.0 18.5 38.0 80-81 30.849059694245156 34.0 31.0 36.0 18.0 37.0 82-83 30.517524650910936 34.0 31.0 35.0 17.5 37.0 84-85 30.106118897262377 34.0 30.0 35.0 11.5 36.5 86-87 29.552725812520535 34.0 29.5 35.0 6.5 36.0 88-89 28.96554855775603 34.0 29.0 35.0 2.0 36.0 90-91 28.48606407697831 34.0 29.0 35.0 2.0 35.0 92-93 28.135115165312023 33.0 28.5 35.0 2.0 35.0 94-95 27.73255669693812 33.0 27.0 35.0 2.0 35.0 96-97 27.093161975188593 33.0 26.5 35.0 2.0 35.0 98-99 26.204208070837332 33.0 25.0 34.0 2.0 35.0 100-101 21.856180928923337 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10910117353995474 1101 2 0.10775989404592679 1101 3 0.15222513955672667 1101 4 0.10870918061865353 1101 5 0.1254134105569662 1101 6 0.046224747216228934 1101 7 0.07222612385788096 1101 8 0.13215010003022343 1101 9 0.2700313535047556 1101 10-11 0.2049936787443869 1101 12-13 0.1666318765844963 1101 14-15 0.19756097389455363 1101 16-17 0.24120470475951095 1101 18-19 0.2716672672117184 1101 20-21 0.2320779870531524 1101 22-23 0.19543462369009745 1101 24-25 0.24711659588077595 1101 26-27 0.2234076359615429 1101 28-29 0.2885729544065825 1101 30-31 0.2556066041240044 1101 32-33 0.2859786704538365 1101 34-35 0.31179179749672414 1101 36-37 0.2936225480021335 1101 38-39 0.2945938421337786 1101 40-41 0.3908299359128691 1101 42-43 0.22679382736816223 1101 44-45 0.2856312425288863 1101 46-47 0.22262964995137935 1101 48-49 0.07125802477771259 1101 50-51 0.19098773647991862 1101 52-53 0.30452923105502094 1101 54-55 0.3239746158130927 1101 56-57 0.19293659903636495 1101 58-59 0.1337653972735282 1101 60-61 0.3541369751020724 1101 62-63 0.2980044778263249 1101 64-65 0.26234578139388276 1101 66-67 0.3504327957693292 1101 68-69 0.22207971546593797 1101 70-71 0.2419517560320017 1101 72-73 0.1919135425129994 1101 74-75 0.25441603052252404 1101 76-77 0.06299814902190803 1101 78-79 0.2603112858475072 1101 80-81 0.43610124965471897 1101 82-83 0.4048933409411468 1101 84-85 0.3290886220819722 1101 86-87 0.4295924485257352 1101 88-89 0.4571724640141923 1101 90-91 0.4823463038311324 1101 92-93 0.2983281854704778 1101 94-95 0.28885639995864665 1101 96-97 0.2759546121521481 1101 98-99 0.4110979339994856 1101 100-101 0.8527026375639046 1102 1 0.14369428462313039 1102 2 0.10373610326716687 1102 3 0.08245528468753349 1102 4 0.04596386853559409 1102 5 0.08853406473722458 1102 6 0.10349573230178066 1102 7 0.08694121009614264 1102 8 0.07976220095358144 1102 9 0.12633156543410706 1102 10-11 0.21226830257529627 1102 12-13 0.18808625002562707 1102 14-15 0.2726035361887753 1102 16-17 0.2140980365932421 1102 18-19 0.10010654316754852 1102 20-21 0.23552602907734865 1102 22-23 0.18685286328715023 1102 24-25 0.20249835540160888 1102 26-27 0.24239565677075348 1102 28-29 0.21148707119487398 1102 30-31 0.16274957153187586 1102 32-33 0.17823012760596413 1102 34-35 0.22752071537796326 1102 36-37 0.20117492939557735 1102 38-39 0.2690846383133163 1102 40-41 0.3071103993787574 1102 42-43 0.23524431727560824 1102 44-45 0.3014288003794192 1102 46-47 0.20424621219708428 1102 48-49 0.04622268484170178 1102 50-51 0.11297391806874657 1102 52-53 0.12340726251583334 1102 54-55 0.1666894616736343 1102 56-57 0.05735419027515576 1102 58-59 -0.014384576373124958 1102 60-61 -0.1191734341354902 1102 62-63 0.1377015908678132 1102 64-65 0.17559985623736196 1102 66-67 0.17377551265509084 1102 68-69 0.1910790935712754 1102 70-71 0.16288882117436998 1102 72-73 0.1753453425010889 1102 74-75 0.24493605134056295 1102 76-77 0.053304917308807376 1102 78-79 0.11311764064061691 1102 80-81 0.27324087603275515 1102 82-83 0.22996677743842397 1102 84-85 0.15527413634695364 1102 86-87 0.2958941380062292 1102 88-89 0.3728369319965452 1102 90-91 0.3953080109981393 1102 92-93 0.2708825626606135 1102 94-95 0.485461064609666 1102 96-97 0.2574975173705525 1102 98-99 0.4302787531803034 1102 100-101 0.9414104529273501 1103 1 -0.02237845793968063 1103 2 0.0875648113508447 1103 3 0.007309869641460409 1103 4 0.045522367431843236 1103 5 0.04217951482306859 1103 6 0.006778082769564264 1103 7 0.05546279459454695 1103 8 0.021866864746989734 1103 9 0.05293594890935083 1103 10-11 0.11025855275926233 1103 12-13 0.04800513295775488 1103 14-15 0.13576513084871067 1103 16-17 0.061444649999458534 1103 18-19 0.020016140560585427 1103 20-21 0.11308239930756514 1103 22-23 0.06968074793622492 1103 24-25 0.08588315339733299 1103 26-27 0.0695545446084509 1103 28-29 0.11944000402363031 1103 30-31 0.15002006103746623 1103 32-33 0.09131222578739795 1103 34-35 0.11861229181722166 1103 36-37 0.07807189495147782 1103 38-39 0.1824363237262574 1103 40-41 0.16784921293213984 1103 42-43 0.2186711629954985 1103 44-45 0.3205164961641387 1103 46-47 0.2609431882649176 1103 48-49 0.09775698877667338 1103 50-51 0.04919781343999574 1103 52-53 0.11776345053924331 1103 54-55 0.20675502984350658 1103 56-57 0.015101702451467247 1103 58-59 0.0964624224705588 1103 60-61 -0.012087078621981107 1103 62-63 0.031530211352055915 1103 64-65 0.10987094141904663 1103 66-67 0.07708808492461827 1103 68-69 0.09393702482324784 1103 70-71 0.04608396641420853 1103 72-73 -0.021141920542468995 1103 74-75 -0.06610257124607344 1103 76-77 -0.062074694800937635 1103 78-79 0.17478916907538888 1103 80-81 0.14126182981529567 1103 82-83 0.22926225906006437 1103 84-85 0.10032433081767778 1103 86-87 0.007690276623559811 1103 88-89 0.18418850353023686 1103 90-91 0.21653225676708487 1103 92-93 0.26220343059572215 1103 94-95 0.004944709796955493 1103 96-97 -0.07902027407273948 1103 98-99 -0.0808984954969425 1103 100-101 0.7049892398505087 1104 1 0.07438407545652126 1104 2 0.07345393597758942 1104 3 0.10106936463857608 1104 4 0.05624844003909857 1104 5 0.08013789399431914 1104 6 0.08726291696726918 1104 7 0.011536318613615038 1104 8 0.10742268223349782 1104 9 0.10079662783438437 1104 10-11 0.045125164420426245 1104 12-13 0.09567263780348156 1104 14-15 0.11105292748552387 1104 16-17 0.11539849776519162 1104 18-19 0.1152110171911076 1104 20-21 0.13216832575734827 1104 22-23 0.09095478010134883 1104 24-25 0.07539903950975457 1104 26-27 0.05941585216777412 1104 28-29 0.054762375546495434 1104 30-31 0.05821653544681027 1104 32-33 0.07605212834908315 1104 34-35 0.1489406222693077 1104 36-37 0.08099046061757065 1104 38-39 0.21229874449214492 1104 40-41 0.16699007939974564 1104 42-43 0.17351393893975597 1104 44-45 0.1613472756706571 1104 46-47 0.19017992146204676 1104 48-49 0.20863170720089386 1104 50-51 0.0227437692260537 1104 52-53 -0.01204396472609659 1104 54-55 0.04734810700836789 1104 56-57 -0.0367642281892131 1104 58-59 0.08165612041178605 1104 60-61 0.19380440338336058 1104 62-63 0.17238620171300312 1104 64-65 0.23041484104710008 1104 66-67 0.20753559396123222 1104 68-69 0.20060251280061436 1104 70-71 0.03663644428104362 1104 72-73 0.14252563668944873 1104 74-75 0.08785618772506609 1104 76-77 -0.001734642938608033 1104 78-79 0.12631458947457475 1104 80-81 0.21944167160900108 1104 82-83 0.3486710154118917 1104 84-85 0.26206604070295114 1104 86-87 0.21014456967191464 1104 88-89 0.24818936790238766 1104 90-91 0.4480985383952465 1104 92-93 0.483633639092762 1104 94-95 0.37483279979643314 1104 96-97 0.23750593211931204 1104 98-99 0.3924899743024177 1104 100-101 0.8733925087488252 1105 1 -0.09753943063231674 1105 2 -0.07815876670222366 1105 3 -0.04974221213456076 1105 4 -0.10049365508628938 1105 5 -0.043196479066239135 1105 6 -0.032910256569635976 1105 7 -0.014654517423544178 1105 8 0.03652947079478963 1105 9 -0.1268311755435434 1105 10-11 -0.06311912217127258 1105 12-13 -0.15355978625088085 1105 14-15 0.007731749313784064 1105 16-17 -0.04051438809955954 1105 18-19 -0.018918243412770153 1105 20-21 -0.031760642651029514 1105 22-23 -0.1275251922869316 1105 24-25 -0.08696396034982712 1105 26-27 -0.07533086706406777 1105 28-29 -0.04741756800810748 1105 30-31 -0.049181927686284155 1105 32-33 -0.13059939260035236 1105 34-35 0.006222786016707005 1105 36-37 0.028265624964149083 1105 38-39 0.05769736197649422 1105 40-41 0.0010107045866547537 1105 42-43 -0.062288639369391774 1105 44-45 -0.06987273168245878 1105 46-47 -0.007092884740302452 1105 48-49 -0.10199553772561387 1105 50-51 0.05108877001388379 1105 52-53 0.11711899054312624 1105 54-55 -1.391831301944535E-4 1105 56-57 0.0696942464987913 1105 58-59 0.0017633730987256513 1105 60-61 -0.1266093544588074 1105 62-63 -0.12887626295503196 1105 64-65 -0.08829183475201319 1105 66-67 0.0010665076242162286 1105 68-69 -0.023535987441093198 1105 70-71 -0.10106497607209519 1105 72-73 -0.007706908487769226 1105 74-75 -0.021081071704028176 1105 76-77 -0.11254185714492593 1105 78-79 -0.09557938621724915 1105 80-81 -0.01594989288034654 1105 82-83 0.05160551152470205 1105 84-85 -0.06841249107357683 1105 86-87 -0.1781380260446248 1105 88-89 -0.0997920070457603 1105 90-91 0.15095473340091203 1105 92-93 0.12400078071111054 1105 94-95 0.01145928154171827 1105 96-97 0.09736774486765398 1105 98-99 0.23482568400200066 1105 100-101 0.7286118858343116 1106 1 0.0037812846209774875 1106 2 -0.03267825550383918 1106 3 -0.050859550999490466 1106 4 -0.018286071661336223 1106 5 -0.018961576642624323 1106 6 -0.008213601071780374 1106 7 -0.01833934711761742 1106 8 -0.0035441222243619563 1106 9 -0.028261849116461235 1106 10-11 0.03460749696868248 1106 12-13 0.01593519285820122 1106 14-15 0.009557254000171156 1106 16-17 -0.07455015541447807 1106 18-19 -0.060139877807280584 1106 20-21 -0.02922221616049825 1106 22-23 0.14187163390842983 1106 24-25 0.11747137398042895 1106 26-27 0.0909076378578959 1106 28-29 0.12797197603794785 1106 30-31 0.12457969633172894 1106 32-33 0.11115349562867038 1106 34-35 0.057941244101755274 1106 36-37 0.03196165763965553 1106 38-39 0.08560768781732975 1106 40-41 0.15789392735043606 1106 42-43 0.1575748914066324 1106 44-45 0.1339803118346694 1106 46-47 0.14399871368946293 1106 48-49 0.10980110660097608 1106 50-51 0.16845521964216204 1106 52-53 0.3139340251978595 1106 54-55 0.1774597790932475 1106 56-57 0.08058956869388822 1106 58-59 0.19564391359396183 1106 60-61 0.1888679237282389 1106 62-63 0.18229975733800785 1106 64-65 0.12048232973544515 1106 66-67 0.1661455568123884 1106 68-69 0.2117711174580208 1106 70-71 0.16015959223528142 1106 72-73 0.2871736354951864 1106 74-75 0.3176026540736139 1106 76-77 0.0977955460060933 1106 78-79 0.3628507357673669 1106 80-81 0.468648808398175 1106 82-83 0.49957997549110544 1106 84-85 0.512098474795696 1106 86-87 0.39343650120805407 1106 88-89 0.5316344484989592 1106 90-91 0.9221752675780941 1106 92-93 0.8240454446729295 1106 94-95 0.7957997223917168 1106 96-97 0.6399918657110426 1106 98-99 0.6077600418233224 1106 100-101 1.0879935425586815 1107 1 -0.09711983549795633 1107 2 -0.054859177023850236 1107 3 -0.08087157593435279 1107 4 -0.05189547294374819 1107 5 -0.1088347356630095 1107 6 -0.03585397218643749 1107 7 -0.09006229195166782 1107 8 -0.03290198403594502 1107 9 -0.03553045557817569 1107 10-11 0.02743649414495053 1107 12-13 0.009713091144583075 1107 14-15 0.024753734185907206 1107 16-17 0.03012880882446467 1107 18-19 -0.0980457662337173 1107 20-21 -0.06505269916204526 1107 22-23 -0.08316647005183597 1107 24-25 -0.0829541549646251 1107 26-27 -0.14459525316810584 1107 28-29 0.01666997475712151 1107 30-31 -0.13025460039916936 1107 32-33 -0.07459737322079008 1107 34-35 -0.15238193956433577 1107 36-37 -0.047337548239653415 1107 38-39 -0.11396180409792578 1107 40-41 -0.18657446719576143 1107 42-43 -0.07447004959993819 1107 44-45 -0.14295308394276418 1107 46-47 -0.20268623135041963 1107 48-49 -0.1530945006311626 1107 50-51 -0.19891226961726716 1107 52-53 -0.11034404121262043 1107 54-55 -0.21963728871430277 1107 56-57 -0.1838620717763888 1107 58-59 -0.1908460241688772 1107 60-61 -0.2119846966534027 1107 62-63 -0.2315745112597014 1107 64-65 -0.2805045607522274 1107 66-67 -0.15943585670495963 1107 68-69 -0.3117051616288009 1107 70-71 -0.2619398922258469 1107 72-73 -0.19745061445679113 1107 74-75 -0.33512177908569996 1107 76-77 -0.59093535878484 1107 78-79 -0.35347574871417464 1107 80-81 -0.15192574682298599 1107 82-83 -0.09250186948223416 1107 84-85 -0.1908761549250606 1107 86-87 -0.1740931850894789 1107 88-89 -0.0019370884052172244 1107 90-91 0.11551770988986121 1107 92-93 0.21746128242047646 1107 94-95 0.10546174297244093 1107 96-97 -0.017270355368594892 1107 98-99 -0.2780439100719363 1107 100-101 0.26523200960032156 1108 1 0.02428493804760734 1108 2 -0.06461312893881654 1108 3 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13 9669.0 14 11744.0 15 15810.0 16 21211.0 17 30310.0 18 29293.0 19 19032.0 20 17119.0 21 17978.0 22 19285.0 23 21084.0 24 24365.0 25 29167.0 26 35125.0 27 42889.0 28 51985.0 29 63502.0 30 77072.0 31 93677.0 32 115654.0 33 148132.0 34 199022.0 35 283939.0 36 446137.0 37 719677.0 38 578801.0 39 44154.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.635617110558385 20.315421559157233 15.301279024992976 21.74768230529141 2 14.975228166582808 22.116784102808605 39.67463373052442 23.233354000084177 3 20.022532411935458 25.765062386576286 30.74568652245169 23.466718679036568 4 12.57578070378767 17.798909373470803 38.59867494090503 31.026634981836505 5 12.793317005052387 38.653017329377526 34.52316415198995 14.030501513580134 6 29.716117992699427 37.70026803959222 17.520944715499244 15.062669252209112 7 26.73844122445159 34.09790888915272 21.532273110170113 17.631376776225576 8 25.11983888728706 34.58386020984604 20.139308976719438 20.156991926147462 9 25.411089313656575 18.272768446331465 21.872489288152927 34.443652951859036 10-11 24.527664236281897 26.965838300577477 27.458997857316504 21.04749960582413 12-13 26.486137258634024 24.769179404357857 27.699916265109813 21.044767071898306 14-15 22.656098477573327 26.070286697018986 27.661278556983937 23.612336268423753 16-17 20.898379926599723 29.364005093952134 28.012166687004157 21.725448292443986 18-19 20.7274162352718 28.68446080195788 29.96295689066535 20.625166072104975 20-21 22.251871785739187 26.807963797380413 29.463641280484243 21.476523136396157 22-23 21.501838335754925 26.94634936608354 29.745893189960103 21.805919108201433 24-25 22.14979436896997 27.050986570380964 29.746631288204433 21.052587772444625 26-27 21.792680452457365 26.57697045534066 30.73458364035079 20.89576545185118 28-29 20.83643491638132 28.057988138918255 30.182360519847308 20.92321642485312 30-31 21.36786565229982 27.008993491543908 29.80492534528864 21.818215510867635 32-33 21.364253682167984 27.310545884898136 29.898349737959418 21.426850694974462 34-35 22.34841584332566 26.849140768697293 29.718312469218937 21.084130918758103 36-37 21.97766972801835 26.51091188618726 30.39275628605276 21.11866209974163 38-39 21.444419035221582 27.319483352316954 30.24240084487062 20.993696767590844 40-41 21.50299464110243 27.403796850306893 29.834232712439544 21.258975796151137 42-43 21.174139507156397 27.61075930730587 29.062721480531543 22.15237970500619 44-45 21.711133100606233 26.91533048028272 29.150914609967483 22.222621809143572 46-47 22.294920753924064 27.650216396917127 28.792199126308933 21.26266372284988 48-49 22.101594312168334 27.489427499635266 28.77906132183698 21.62991686635942 50-51 21.122945523975602 27.81940115728141 29.368567714279703 21.689085604463287 52-53 21.8613549976161 28.442521619996874 28.009948936164868 21.68617444622216 54-55 21.19331326962446 27.454946169081662 28.63353202309902 22.718208538194855 56-57 22.07022109654388 27.627786791998886 28.154820346887327 22.147171764569904 58-59 22.815813302821017 27.302345492926648 28.057390248475823 21.824450955776513 60-61 21.91814253380732 27.618021733927428 29.155456190726863 21.308379541538386 62-63 21.6160080003568 27.626687496741376 28.951024197687207 21.806280305214617 64-65 21.27756606700502 28.30372715306087 28.532412477845227 21.886294302088885 66-67 21.6278732669548 27.672673986093077 27.911318842625843 22.788133904326276 68-69 21.26912943304151 27.78184087622214 28.449516922285902 22.49951276845045 70-71 22.041293927053278 27.623530948930515 28.090009039347862 22.245166084668348 72-73 21.810222063923703 27.05046833118814 28.848067344711982 22.291242260176176 74-75 21.113834222506153 28.693365093543743 27.959208607921582 22.233592076028515 76-77 21.511634946145005 27.431336670879087 28.141347405555834 22.91568097742007 78-79 21.556465598537873 27.56022613470559 28.36924551671567 22.51406275004087 80-81 21.850126481771486 27.70154950377812 28.08473242211179 22.3635915923386 82-83 21.59273434933342 27.42771505513123 28.623716886871208 22.355833708664143 84-85 21.22783114071042 27.753279197753166 28.282794019079997 22.736095642456416 86-87 21.326205489442152 27.783021100911228 28.121898272051983 22.768875137594637 88-89 21.262532624020174 27.597957238675804 27.768755203359678 23.370754933944344 90-91 21.390792540406864 27.020658394232587 28.200636071991514 23.387912993369035 92-93 21.785597850155376 27.742710259063063 28.02714505483599 22.444546835945573 94-95 21.366106779887797 27.333050029241253 28.095426994545612 23.205416196325338 96-97 20.54735059421844 27.330703180995563 28.753652029652187 23.36829419513381 98-99 21.018525323679636 27.671161842016822 27.616746726769847 23.69356610753369 100-101 20.948978667209825 26.750688512932985 28.546907529851 23.753425290006184 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1663.0 1 1113.5 2 662.0 3 770.0 4 686.5 5 679.0 6 1172.0 7 2390.5 8 3899.5 9 4669.5 10 4245.0 11 3761.0 12 3938.5 13 4184.0 14 4408.0 15 4651.0 16 4874.5 17 5160.0 18 5465.5 19 5908.0 20 6640.0 21 7805.5 22 9310.0 23 11157.5 24 13659.0 25 16646.0 26 20718.0 27 25172.5 28 28350.5 29 32516.0 30 38970.5 31 45790.5 32 52864.0 33 60763.5 34 68221.5 35 74316.0 36 82533.0 37 90628.5 38 93953.0 39 95052.5 40 96277.0 41 99113.0 42 101810.0 43 105106.5 44 108678.5 45 111152.0 46 113142.0 47 134619.5 48 187478.5 49 183847.0 50 131898.5 51 109000.0 52 97896.5 53 91528.0 54 86246.5 55 78383.5 56 70182.0 57 62125.5 58 55180.0 59 47850.0 60 40416.5 61 33236.5 62 26471.0 63 20500.5 64 15075.5 65 11109.0 66 7754.5 67 5974.5 68 4354.0 69 2557.0 70 1699.5 71 1266.0 72 958.5 73 741.0 74 572.5 75 466.0 76 319.0 77 145.5 78 72.5 79 48.0 80 26.0 81 15.5 82 9.0 83 4.0 84 6.0 85 3.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40601685125404463 2 0.0 3 0.0 4 0.0 5 5.025349748638287E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.323235521182163E-4 16-17 0.002591195964141617 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0025754917461771223 36-37 0.007616545712779905 38-39 0.012940275602743589 40-41 0.0027482381437865635 42-43 8.951404239761949E-4 44-45 4.5542232097034474E-4 46-47 2.9838014132539827E-4 48-49 2.6697170539640905E-4 50-51 5.339434107928181E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.1985905150292506E-4 60-61 0.002512674874319144 62-63 0.0 64-65 2.1985905150292506E-4 66-67 0.0014919007066269915 68-69 0.0016803513222009272 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12489564547162593 78-79 1.0992952575146253E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14707000123749236 88-89 0.4406917645196488 90-91 0.33888131945583 92-93 0.0 94-95 0.0 96-97 3.769012311478716E-4 98-99 0.0 100-101 1.0992952575146253E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3183858.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.40800340439495 #Duplication Level Percentage of deduplicated Percentage of total 1 67.96749373029617 28.143982117722967 2 15.550184244848772 12.878041642993331 3 6.271552237325843 7.790773691820878 4 3.229818760358731 5.34961384898052 5 1.883943718745484 3.9005173959750747 6 1.1756748186085564 2.920940813484272 7 0.7530686978864506 2.1828149844077793 8 0.5492902459182105 1.819600989838576 9 0.4027417833942215 1.5009059824092013 >10 1.9762823746705245 14.388331626933889 >50 0.14466567863818766 4.152786480081959 >100 0.086703799916846 6.558481500193222 >500 0.004707560905556733 1.2790912236669891 >1k 0.002733422461039307 2.319549544289672 >5k 7.59284016955363E-4 2.2428663229368944 >10k+ 3.796420084776815E-4 2.5717018342647746 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 30027 0.9431011056397616 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14451 0.4538833076098243 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14136 0.4439896502921927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12212 0.3835598195648173 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10970 0.34455054214101255 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9530 0.29932239440326797 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8865 0.2784357845104901 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7771 0.2440749556041758 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7638 0.23989763362562022 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7302 0.22934439915347984 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6812 0.2139542655482751 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6594 0.20710722651575542 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6488 0.20377793230728253 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5281 0.16586795014099248 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5056 0.15880105205696987 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4990 0.1567280952856566 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4058 0.12745543299983855 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3714 0.11665093104026623 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3693 0.11599135388575747 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3509 0.11021220167482344 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3189 0.10016150217754685 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.14084359289893E-5 0.0 10-11 0.0 0.0 0.0 4.711265389348395E-5 0.0 12-13 0.0 0.0 0.0 1.256337437159572E-4 0.0 14-15 0.0 0.0 0.0 2.1985905150292506E-4 0.0 16-17 0.0 0.0 0.0 4.397181030058501E-4 0.0 18-19 0.0 0.0 0.0 5.496476287573127E-4 0.0 20-21 0.0 0.0 0.0 7.223940263667537E-4 0.0 22-23 0.0 0.0 0.0 0.0010835910395501306 0.0 24-25 0.0 0.0 0.0 0.0015704217964494648 0.0 26-27 0.0 0.0 0.0 0.002371336912638692 0.0 28-29 0.0 0.0 0.0 0.005449363633679642 0.0 30-31 0.0 0.0 0.0 0.015123161899808345 0.0 32-33 0.0 0.0 0.0 0.03357561800808956 0.0 34-35 0.0 0.0 0.0 0.056440959364393764 0.0 36-37 0.0 0.0 0.0 0.09424101200493237 0.0 38-39 0.0 0.0 0.0 0.15178126662684077 0.0 40-41 0.0 0.0 0.0 0.21850848875797851 0.0 42-43 0.0 0.0 0.0 0.28814099121254777 0.0 44-45 0.0 0.0 0.0 0.37105926206507955 0.0 46-47 0.0 0.0 0.0 0.4741260445660579 0.0 48-49 0.0 0.0 0.0 0.5829248666240768 0.0 50-51 0.0 0.0 0.0 0.7037531196428986 0.0 52-53 0.0 0.0 0.0 0.8222728526209397 0.0 54-55 0.0 0.0 0.0 0.9594177881048715 0.0 56-57 0.0 0.0 0.0 1.1340329876520876 0.0 58-59 0.0 0.0 0.0 1.3186203656067577 0.0 60-61 0.0 0.0 0.0 1.5268425915979922 0.0 62-63 0.0 0.0 0.0 1.7420688988013913 0.0 64-65 0.0 0.0 0.0 1.9620849924839612 0.0 66-67 0.0 0.0 0.0 2.215912267444088 0.0 68-69 0.0 0.0 0.0 2.5112771989203035 0.0 70-71 0.0 0.0 0.0 2.8346270468092483 0.0 72-73 0.0 0.0 0.0 3.163457666767802 0.0 74-75 0.0 0.0 0.0 3.515954543198849 0.0 76-77 0.0 0.0 0.0 3.8818471175536096 0.0 78-79 0.0 0.0 0.0 4.327140217936855 0.0 80-81 0.0 0.0 0.0 4.8091026672671955 0.0 82-83 0.0 0.0 0.0 5.288206948927998 0.0 84-85 0.0 0.0 0.0 5.788040798301934 0.0 86-87 0.0 0.0 0.0 6.309891961262092 0.0 88-89 0.0 0.0 0.0 6.8804733125660755 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTGCG 875 0.0 22.776762 2 GTACATG 14210 0.0 22.765793 1 GAGTACT 12290 0.0 22.278622 12-13 TACATGG 14545 0.0 21.531828 2 AGTACTT 12815 0.0 21.051168 12-13 ACATGGG 14635 0.0 20.686102 3 TTTGCGT 990 0.0 20.610285 3 GTACTTT 13305 0.0 20.382889 14-15 TTGCGTT 1085 0.0 19.680382 4 CATGGGG 5150 0.0 19.533468 4 ACTTTTT 15545 0.0 17.537062 16-17 TACACCG 245 3.8027174E-6 17.431194 5 TACTTTT 15595 0.0 17.11597 14-15 CATGGGA 9335 0.0 16.825361 4 AGAGTAC 25885 0.0 16.673065 10-11 GTATAGT 1150 0.0 16.157488 1 GTAAGCG 355 0.0 16.039972 94-95 GTACACG 510 3.6379788E-12 15.88129 1 AGTACAT 11265 0.0 15.459148 2 GTGTAGC 1575 0.0 15.125039 1 >>END_MODULE