##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938987_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1704770 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.796150213811835 33.0 31.0 34.0 30.0 34.0 2 31.79513541416144 33.0 31.0 34.0 30.0 34.0 3 31.84887932096412 33.0 31.0 34.0 30.0 34.0 4 35.273768895510834 37.0 35.0 37.0 33.0 37.0 5 35.35613484516973 37.0 35.0 37.0 33.0 37.0 6 35.5060764795251 37.0 35.0 37.0 33.0 37.0 7 35.47956557189533 37.0 35.0 37.0 33.0 37.0 8 35.51069997712301 37.0 35.0 37.0 33.0 37.0 9 36.985393924107065 39.0 37.0 39.0 33.0 39.0 10-11 36.89375018330918 39.0 37.0 39.0 32.5 39.0 12-13 36.810625773564766 39.0 37.0 39.0 32.5 39.0 14-15 37.6360374126715 40.0 37.0 41.0 32.0 41.0 16-17 37.595521389982224 39.5 37.0 41.0 32.0 41.0 18-19 37.74408600573685 40.0 37.0 41.0 32.5 41.0 20-21 37.87076233157552 40.0 38.0 41.0 32.5 41.0 22-23 37.92105474638808 40.0 38.0 41.0 33.0 41.0 24-25 37.823459176311175 40.0 37.5 41.0 32.5 41.0 26-27 37.37005050534676 39.5 36.5 41.0 31.0 41.0 28-29 37.15839937352253 39.0 36.5 41.0 30.5 41.0 30-31 37.19631563202074 39.0 36.5 41.0 31.0 41.0 32-33 37.11481314194877 39.5 36.0 41.0 31.0 41.0 34-35 36.87414196636496 39.5 36.0 41.0 30.5 41.0 36-37 36.384880658387935 39.0 35.0 41.0 30.0 41.0 38-39 35.80939188277598 38.5 34.5 40.0 28.5 41.0 40-41 35.57342544742106 38.0 35.0 40.0 29.0 41.0 42-43 35.69980554561612 38.0 35.0 40.0 29.0 41.0 44-45 35.85982830528458 39.0 35.0 40.0 29.5 41.0 46-47 35.587593341037206 39.0 35.0 40.5 27.0 41.0 48-49 35.37199446259613 39.0 35.0 40.0 26.5 41.0 50-51 34.194282513183595 38.0 33.5 39.5 24.5 40.5 52-53 33.97992515119341 37.5 33.5 39.5 23.5 40.0 54-55 34.557774655818676 38.0 34.0 40.0 24.0 41.0 56-57 34.779602820321806 38.0 34.0 40.0 24.5 41.0 58-59 34.70474228195006 38.0 34.0 40.0 24.0 41.0 60-61 34.272101808455105 38.0 34.0 40.0 22.5 41.0 62-63 33.62652615895399 37.5 33.0 39.5 22.0 41.0 64-65 32.80075699361204 36.0 31.5 39.0 19.5 41.0 66-67 32.344360236278206 36.0 31.0 39.0 18.0 40.0 68-69 32.04131231779067 36.0 31.0 38.0 18.0 39.5 70-71 31.302229039694506 35.0 30.5 37.0 12.0 39.0 72-73 30.599937528229617 35.0 30.0 36.5 8.0 39.0 74-75 30.108292320958252 34.0 30.0 36.0 4.5 37.0 76-77 29.34591117863407 34.0 29.0 35.0 2.0 37.0 78-79 28.877710776233744 34.0 29.0 35.0 2.0 36.0 80-81 28.665494465529076 34.0 29.0 35.0 2.0 36.0 82-83 28.239567800935024 34.0 28.0 35.0 2.0 35.5 84-85 27.874938261466355 34.0 27.0 35.0 2.0 35.0 86-87 27.16247792957408 33.0 26.0 35.0 2.0 35.0 88-89 26.29916616317744 32.5 24.0 35.0 2.0 35.0 90-91 25.56669580060653 31.5 21.5 34.0 2.0 35.0 92-93 25.235018800190055 32.0 20.0 34.0 2.0 35.0 94-95 24.692916639781316 32.0 16.5 34.0 2.0 35.0 96-97 23.700325557113274 31.0 2.0 34.0 2.0 35.0 98-99 22.623980067692415 31.0 2.0 34.0 2.0 35.0 100-101 19.430616446793408 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1413760675641491 1101 2 0.29448285385070605 1101 3 0.3600010558812805 1101 4 0.29380026179926944 1101 5 0.1310513894101888 1101 6 0.20324876066080577 1101 7 0.2800289990207361 1101 8 0.3430993491409353 1101 9 0.514056783408698 1101 10-11 0.29097294051106104 1101 12-13 0.366247571941237 1101 14-15 0.4965746744463786 1101 16-17 0.5074369761928992 1101 18-19 0.572641520253697 1101 20-21 0.4210466490806297 1101 22-23 0.6210855863508371 1101 24-25 0.5330460402774548 1101 26-27 0.619824910653449 1101 28-29 0.6753773132081449 1101 30-31 0.5740027812702024 1101 32-33 0.49739452552420715 1101 34-35 0.552305545098271 1101 36-37 0.6054260400412304 1101 38-39 0.8407599094045466 1101 40-41 0.6540220334722662 1101 42-43 0.6263415112868813 1101 44-45 0.839783590041769 1101 46-47 0.5701123648004582 1101 48-49 0.6634209604246735 1101 50-51 0.5780702912777826 1101 52-53 0.606085202548492 1101 54-55 0.812295642668964 1101 56-57 0.8945018204221995 1101 58-59 0.854667474554013 1101 60-61 0.9353381096975326 1101 62-63 0.8041313932204872 1101 64-65 0.6427992857388674 1101 66-67 0.781231122726318 1101 68-69 0.7736534673883568 1101 70-71 0.666400648060236 1101 72-73 0.6941678255414132 1101 74-75 0.5703795396015643 1101 76-77 0.6356746218559266 1101 78-79 0.5698106300294903 1101 80-81 0.5575426729362718 1101 82-83 0.6437982355794958 1101 84-85 0.6765829803942331 1101 86-87 0.6036496276971093 1101 88-89 0.5385798891256428 1101 90-91 0.7184779521492892 1101 92-93 0.8484490859887082 1101 94-95 0.81119528287023 1101 96-97 0.8486423317020737 1101 98-99 0.7163576562555143 1101 100-101 1.201786834395751 1102 1 0.275527187664828 1102 2 0.38850001387762845 1102 3 0.3846602617138082 1102 4 0.16175674875771762 1102 5 0.32112787298670753 1102 6 0.3948735326246293 1102 7 0.5027996100763801 1102 8 0.4841574044679575 1102 9 0.605057443534804 1102 10-11 0.5006662257603338 1102 12-13 0.4939573814802216 1102 14-15 0.6322100620834803 1102 16-17 0.7673915071619106 1102 18-19 0.6533598220061236 1102 20-21 0.642990273335549 1102 22-23 0.6872240030887724 1102 24-25 0.6887831621276277 1102 26-27 0.8810521354897958 1102 28-29 0.823149631486487 1102 30-31 0.7333248675288573 1102 32-33 0.7410286172979923 1102 34-35 0.7860661989808193 1102 36-37 0.8681846842787806 1102 38-39 0.8417318068931436 1102 40-41 0.6944523186272349 1102 42-43 0.6504934280914796 1102 44-45 0.8591621935010849 1102 46-47 0.8422107365977993 1102 48-49 0.9285343525298231 1102 50-51 0.9675530271915704 1102 52-53 1.047294143842798 1102 54-55 1.142093873335888 1102 56-57 0.9217021459326631 1102 58-59 1.0480683678379634 1102 60-61 1.0677179862572004 1102 62-63 1.0752916055014339 1102 64-65 1.0358348740152863 1102 66-67 1.089167553882966 1102 68-69 1.2404337592096155 1102 70-71 1.1815067234967138 1102 72-73 1.1098911839248515 1102 74-75 1.2426423738513783 1102 76-77 1.0975911581774973 1102 78-79 1.075084931938001 1102 80-81 1.041760415370021 1102 82-83 1.112271116753547 1102 84-85 0.9829883986312744 1102 86-87 0.9770344730877696 1102 88-89 1.1213631173699383 1102 90-91 1.1652315056113736 1102 92-93 1.17616531793686 1102 94-95 1.213839486280122 1102 96-97 1.4126235506265523 1102 98-99 1.0009069657245853 1102 100-101 1.1422480527999355 1103 1 0.2711684303935229 1103 2 0.3485794791089738 1103 3 0.3388164526621402 1103 4 0.34061428113452763 1103 5 0.2056598377432266 1103 6 0.26928643634889227 1103 7 0.30733558270055283 1103 8 0.22998938306690775 1103 9 0.31833515496189335 1103 10-11 0.32548719962388617 1103 12-13 0.38786900397450097 1103 14-15 0.5641571936413428 1103 16-17 0.5919585689281419 1103 18-19 0.595651557750827 1103 20-21 0.6432299003624209 1103 22-23 0.5662332791294489 1103 24-25 0.68693254131054 1103 26-27 0.7543554312768634 1103 28-29 0.6345162821646042 1103 30-31 0.6443449744697034 1103 32-33 0.58441273841607 1103 34-35 0.546111822679066 1103 36-37 0.7252685903562579 1103 38-39 0.9536881291836465 1103 40-41 0.8773977955312873 1103 42-43 0.6652352184011292 1103 44-45 0.8561152483332322 1103 46-47 0.8484026104449285 1103 48-49 0.7545444076098349 1103 50-51 0.7298826525766628 1103 52-53 0.800886939660451 1103 54-55 0.7600116975627245 1103 56-57 0.9317566223506262 1103 58-59 0.9397645972231956 1103 60-61 1.1423598937446755 1103 62-63 0.9387001762217864 1103 64-65 0.8723655816856066 1103 66-67 0.8714522154998292 1103 68-69 0.8694321705488264 1103 70-71 0.8324689336506204 1103 72-73 0.7426558239088834 1103 74-75 0.9311443407647424 1103 76-77 1.0112262378036867 1103 78-79 0.9996167035442696 1103 80-81 1.038601201620299 1103 82-83 1.2535729977150467 1103 84-85 1.0927772129784756 1103 86-87 1.3126881579116336 1103 88-89 1.3855399432031348 1103 90-91 1.2899345371045214 1103 92-93 1.1033259449025685 1103 94-95 0.770598899940353 1103 96-97 0.7584834152926163 1103 98-99 1.1098080937220338 1103 100-101 1.069711483019578 1104 1 0.31648802277577204 1104 2 0.3587461681026092 1104 3 0.3013425830038692 1104 4 0.3564759048139905 1104 5 0.2513139452304429 1104 6 0.3015470886494498 1104 7 0.26640791273261044 1104 8 0.28510843773267425 1104 9 0.37031116956684684 1104 10-11 0.4408694035760661 1104 12-13 0.4544213487735149 1104 14-15 0.46109655567157404 1104 16-17 0.5216054471506268 1104 18-19 0.6070061842621826 1104 20-21 0.637897324588323 1104 22-23 0.6318954009599267 1104 24-25 0.6269328372726832 1104 26-27 0.6239083648847696 1104 28-29 0.7293187975312705 1104 30-31 0.7782199444840714 1104 32-33 0.7768706884265129 1104 34-35 0.8371654712691949 1104 36-37 0.9407370127534236 1104 38-39 1.067392110964839 1104 40-41 0.9332271593372994 1104 42-43 0.9365939494400806 1104 44-45 0.9230135962413968 1104 46-47 0.9330970451105074 1104 48-49 1.0333463918023398 1104 50-51 0.886011833820767 1104 52-53 0.9532683055724647 1104 54-55 1.0989806433455769 1104 56-57 1.077679782790149 1104 58-59 1.1998728726362202 1104 60-61 1.0412126899638352 1104 62-63 1.1600148244785657 1104 64-65 1.0265125954275973 1104 66-67 1.1013598908885527 1104 68-69 1.2076758143724078 1104 70-71 0.9689845869325318 1104 72-73 1.090368114437787 1104 74-75 1.0058255194417605 1104 76-77 1.0804395159115217 1104 78-79 1.0979723223076476 1104 80-81 1.2002802571583757 1104 82-83 1.1421000252893734 1104 84-85 1.2541172750370606 1104 86-87 1.4156732476504672 1104 88-89 1.3061843150935886 1104 90-91 1.3221504056599436 1104 92-93 1.4003231753200005 1104 94-95 1.2248542514031833 1104 96-97 1.4479246932882965 1104 98-99 1.5753279200701407 1104 100-101 1.5129784611214951 1105 1 0.14098929137128735 1105 2 0.23544591869361753 1105 3 0.29845519572569046 1105 4 0.35604720303764736 1105 5 0.3166574858222262 1105 6 0.40749376723048414 1105 7 0.25681061147571427 1105 8 0.3910320264514269 1105 9 0.42480285971782905 1105 10-11 0.4602635409830711 1105 12-13 0.4358761486363605 1105 14-15 0.56183685513939 1105 16-17 0.45663557749674766 1105 18-19 0.5913651115533582 1105 20-21 0.5485703440457712 1105 22-23 0.5131413530184474 1105 24-25 0.6787651525355045 1105 26-27 0.46227682751940335 1105 28-29 0.6472978342453075 1105 30-31 0.65130209090227 1105 32-33 0.6178808237215563 1105 34-35 0.8048810334130607 1105 36-37 0.7866460471623213 1105 38-39 0.9139815266489322 1105 40-41 0.9227408983826066 1105 42-43 0.6407408502225564 1105 44-45 0.6447184131330133 1105 46-47 0.5790676909685928 1105 48-49 0.6447174562796292 1105 50-51 0.7282093652835897 1105 52-53 0.7841133214140328 1105 54-55 0.8587046502217319 1105 56-57 0.7734138922507228 1105 58-59 0.7609800007460947 1105 60-61 0.8771930835921111 1105 62-63 0.7218910374178904 1105 64-65 0.8708771104654431 1105 66-67 0.8238917096808507 1105 68-69 0.8843490501048876 1105 70-71 0.6050383293566526 1105 72-73 0.5565495476486291 1105 74-75 0.6708728171004843 1105 76-77 0.5292475594959498 1105 78-79 0.6172692898775516 1105 80-81 0.6874728877979273 1105 82-83 0.6808346161735734 1105 84-85 0.7205324931034944 1105 86-87 0.6859016025401132 1105 88-89 0.5826369271919845 1105 90-91 0.6597823016878372 1105 92-93 0.7938338849753741 1105 94-95 0.83163947681604 1105 96-97 0.6949856424432674 1105 98-99 0.7786853399781428 1105 100-101 1.10362425763131 1106 1 0.1915464939967393 1106 2 0.26253629689223246 1106 3 0.3184687756489204 1106 4 0.2914462085211582 1106 5 0.19230258819826673 1106 6 0.27858533759398796 1106 7 0.2208607899643127 1106 8 0.15860357315090567 1106 9 0.18724271925749747 1106 10-11 0.23616007362082314 1106 12-13 0.3387292770607502 1106 14-15 0.3641234811559215 1106 16-17 0.47035891905422744 1106 18-19 0.5202705159594387 1106 20-21 0.46833731166683634 1106 22-23 0.3418309576544445 1106 24-25 0.38502816382745664 1106 26-27 0.403851784886065 1106 28-29 0.3471445559039381 1106 30-31 0.3468927615398343 1106 32-33 0.4108962389512456 1106 34-35 0.35311048929735733 1106 36-37 0.2478329010795477 1106 38-39 0.5770425668351962 1106 40-41 0.6241943535318839 1106 42-43 0.541090880586907 1106 44-45 0.7387005575507928 1106 46-47 0.6494698449326464 1106 48-49 0.6311140034833045 1106 50-51 0.5073322501433211 1106 52-53 0.5529750728655856 1106 54-55 0.6436998434667274 1106 56-57 0.781483974514579 1106 58-59 0.8183338877189996 1106 60-61 0.8993786669125541 1106 62-63 0.9529009937873525 1106 64-65 0.7422796072889497 1106 66-67 0.8895851485162964 1106 68-69 0.9744000756580036 1106 70-71 0.9249273146047265 1106 72-73 0.9097435187005303 1106 74-75 0.928759144627616 1106 76-77 0.925969554265663 1106 78-79 0.9811602516095093 1106 80-81 1.05512409889851 1106 82-83 1.307512218487986 1106 84-85 1.2810875220448636 1106 86-87 1.3529307366295313 1106 88-89 1.5453862565992402 1106 90-91 1.4008735583237346 1106 92-93 1.3266643577852726 1106 94-95 1.0989460357396865 1106 96-97 1.2896081273003084 1106 98-99 1.4887360518036594 1106 100-101 1.5859361835378145 1107 1 0.08083578631187294 1107 2 0.23570950724006323 1107 3 0.1995014566293456 1107 4 0.11148418087245204 1107 5 0.09017812294916183 1107 6 0.1187941423429777 1107 7 0.10648383592841526 1107 8 0.022868251091239244 1107 9 0.08969494433940639 1107 10-11 0.025231435155610882 1107 12-13 0.14796283437049595 1107 14-15 0.2815915544405456 1107 16-17 0.1763067452162872 1107 18-19 0.33986154308419714 1107 20-21 0.1942490511402326 1107 22-23 0.24103221399516173 1107 24-25 0.2713378212989994 1107 26-27 0.21942750215758622 1107 28-29 0.25414252341872157 1107 30-31 0.3080988515104153 1107 32-33 0.21760862214092214 1107 34-35 0.26183758065555196 1107 36-37 0.3642394614581761 1107 38-39 0.578612992735863 1107 40-41 0.3547907411204818 1107 42-43 0.4098419241788207 1107 44-45 0.09068071922837362 1107 46-47 0.3217441987693306 1107 48-49 0.5431525199418274 1107 50-51 0.4438667476933915 1107 52-53 0.40527550930291767 1107 54-55 0.44482181273187393 1107 56-57 0.374380928089856 1107 58-59 0.47628692176456155 1107 60-61 0.44578791903860093 1107 62-63 0.27057936883556977 1107 64-65 0.11248664153028187 1107 66-67 0.0101736335950946 1107 68-69 0.07411888161612268 1107 70-71 0.1366663561699184 1107 72-73 0.21280261047328608 1107 74-75 0.13468897782564326 1107 76-77 0.25634988234217104 1107 78-79 0.5351261887694392 1107 80-81 0.32433158800196793 1107 82-83 0.37021481926297994 1107 84-85 0.4588720411865026 1107 86-87 0.583156829018364 1107 88-89 0.6289880449559213 1107 90-91 0.7292753800753573 1107 92-93 0.5946116196879778 1107 94-95 0.4871661861320753 1107 96-97 0.4427224934585645 1107 98-99 0.5639883851312355 1107 100-101 0.7612463102658573 1108 1 0.2412654824452929 1108 2 0.14276498215479094 1108 3 0.2825637471872007 1108 4 0.2286366338469037 1108 5 0.23756640553850872 1108 6 0.2598745203393804 1108 7 0.2729391937646142 1108 8 0.29699105810878734 1108 9 0.41795387904422654 1108 10-11 0.4044098246089831 1108 12-13 0.36511439133577284 1108 14-15 0.5585431370914264 1108 16-17 0.45658326858121967 1108 18-19 0.495528203777468 1108 20-21 0.3813544337660204 1108 22-23 0.4671199691337833 1108 24-25 0.4930440915712069 1108 26-27 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2316 88-89 -3.4354225079414746 2316 90-91 -3.6500144638526457 2316 92-93 -3.7942796425585463 2316 94-95 -3.9783928345256285 2316 96-97 -4.247913898658091 2316 98-99 -4.131107545984435 2316 100-101 -3.942686818009374 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5000.0 3 1340.0 4 1230.0 5 1270.0 6 1604.0 7 2041.0 8 2461.0 9 3203.0 10 4324.0 11 5805.0 12 8219.0 13 11437.0 14 10379.0 15 10912.0 16 12939.0 17 16416.0 18 14753.0 19 12439.0 20 12753.0 21 13981.0 22 15452.0 23 17581.0 24 19869.0 25 23117.0 26 26422.0 27 30990.0 28 36329.0 29 42999.0 30 50895.0 31 61653.0 32 75814.0 33 96066.0 34 127919.0 35 184482.0 36 257174.0 37 309540.0 38 162363.0 39 13580.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.88921068534211 14.231000295971667 12.099239358700595 18.78054965998563 2 11.687814569303145 23.392126322636777 39.98118968767484 24.938869420385235 3 29.183852847734297 28.93560855374213 29.28037924776342 12.600159350760146 4 11.527702750311201 12.519317941611668 41.4365150198464 34.516464288230736 5 12.6260286208069 42.31531510898844 32.60609776221205 12.452558507992602 6 23.973164713864154 35.56884421166798 19.650784446755516 20.807206627712347 7 24.717358858436743 39.9017160658959 17.594620148969476 17.786304926697884 8 17.657702707382164 28.735085745409002 22.11790778736993 31.489303759838904 9 24.304617687194614 26.78892459391645 22.577511701955952 26.328946016932985 10-11 21.623843184944853 21.74457218385084 27.874342065105623 28.757242566098682 12-13 37.772772117717004 19.472852347831292 22.941058744055372 19.813316790396332 14-15 31.659830803961427 21.630553533212257 30.499128020346483 16.210487642479833 16-17 19.06939891832884 25.927006271110344 34.69291514192899 20.310679668631828 18-19 17.4038549299193 35.08695265550654 30.597526756977185 16.911665657596973 20-21 20.26942710788178 30.86948380837113 23.284502042332843 25.57658704141425 22-23 18.63990517888152 28.31518746426303 20.46673218200113 32.57817517485432 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25.42233909204058 24.329101321593694 26.557575725423515 50-51 30.17647299797206 27.65184726519755 24.935066695706016 17.236613041124375 52-53 21.094121388218493 27.34398692404019 32.258464759480795 19.303426928260524 54-55 15.752035365422762 25.959967242013914 34.659723325271536 23.628274067291787 56-57 22.477363713824076 33.25719158023257 22.85089337169 21.41455133425336 58-59 19.439179543498227 27.80296812759567 25.933468410692516 26.824383918213584 60-61 20.457589127966415 22.124689636326803 29.608341067886606 27.809380167820173 62-63 32.71386429809242 20.568448391544347 25.408622374846736 21.309064935516503 64-65 28.212332489616625 22.92597771126026 30.952800593234624 17.90888920588849 66-67 19.594736795564476 24.895346060997287 33.693852225867396 21.816064917570838 68-69 18.135926588887866 33.136527971360046 29.59149621600715 19.136049223744937 70-71 19.76366379485692 30.717634067410586 23.247951381457813 26.27075075627468 72-73 19.12879133990791 27.45419089294378 21.315007135980967 32.102010631167346 74-75 21.905935049071623 27.814337827831935 25.933032914066885 24.346694209029557 76-77 27.221909854094616 21.428247272967308 28.387573406509986 22.962269466428086 78-79 21.32853400634692 22.117788966517356 33.50715523791818 23.046521789217543 80-81 19.368702427054448 32.28376362420802 28.6612586763123 19.68627527242523 82-83 19.099832330953355 29.996935398795966 24.882941074201657 26.020291196049023 84-85 21.25772571281064 23.837511175652075 25.39644465451691 29.508318457020373 86-87 25.055259160379013 24.276577217696488 28.270412590926036 22.397751030998467 88-89 28.977891752366176 18.510769154602137 30.436598180735313 22.074740912296377 90-91 21.11746524278838 22.425670364494252 34.42884877300876 22.028015619708608 92-93 18.33804587886346 28.95113267625917 30.425791482166066 22.285029962711302 94-95 18.842454705595213 31.063248274414647 25.389656863785465 24.70464015620468 96-97 17.995666024508893 26.14509621755708 22.50003536976734 33.35920238816669 98-99 21.94303682551211 24.666867886087797 24.794938938551834 28.59515634984826 100-101 27.143490734096492 25.03840594338052 25.975767345296912 21.842335977226078 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7576.0 1 4556.5 2 1676.0 3 1674.5 4 1254.5 5 1218.5 6 2189.0 7 3730.0 8 4979.5 9 4871.5 10 3439.0 11 2306.5 12 2090.0 13 2108.5 14 2112.5 15 2669.0 16 3197.5 17 2758.5 18 2169.0 19 2026.0 20 2125.5 21 2300.5 22 2512.0 23 2926.5 24 3435.5 25 3963.5 26 4714.0 27 5593.0 28 6303.5 29 6945.0 30 7906.5 31 9003.5 32 10277.5 33 11677.0 34 13213.5 35 14358.5 36 15562.0 37 16995.0 38 16824.5 39 16019.5 40 15940.0 41 16113.5 42 16432.5 43 17222.5 44 21290.5 45 27757.5 46 51957.0 47 117715.0 48 416334.5 49 422572.0 50 133430.0 51 65892.5 52 30135.5 53 17836.5 54 13240.0 55 10356.0 56 8931.0 57 8221.0 58 9255.5 59 9773.0 60 10227.5 61 10956.5 62 9284.5 63 5698.5 64 2606.0 65 1799.0 66 1247.0 67 824.5 68 709.0 69 618.0 70 531.0 71 462.0 72 374.5 73 361.5 74 327.0 75 291.5 76 240.5 77 126.5 78 61.0 79 39.0 80 27.5 81 18.5 82 10.5 83 15.0 84 11.5 85 4.0 86 3.5 87 3.0 88 2.0 89 2.0 90 2.5 91 2.0 92 2.0 93 1.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.309895176475419 2 0.08499680308780656 3 0.09438223338045601 4 0.0991336074661098 5 0.1104547827566182 6 0.0524410917601788 7 0.054611472515353976 8 0.05555001554461892 9 0.05836564463241376 10-11 0.055960628119922334 12-13 0.05402488312206339 14-15 0.05211846759386896 16-17 0.054259518879379624 18-19 0.06191451046182182 20-21 0.06341031341471283 22-23 0.07930688597288783 24-25 0.08734316066096892 26-27 0.08285575180229592 28-29 0.08042140582013996 30-31 0.07514210128052465 32-33 0.06628460144183672 34-35 0.30766613678091476 36-37 0.4792435343184125 38-39 0.454460132451885 40-41 0.4368624506531673 42-43 0.4660746024390387 44-45 0.47463880758108135 46-47 0.4698287745560985 48-49 0.48660523120420934 50-51 0.482264469693859 52-53 0.482264469693859 54-55 0.4742281950057779 56-57 0.45261237586301967 58-59 0.43824093572740014 60-61 0.39665174774309736 62-63 0.4657813077423934 64-65 0.48733846794582264 66-67 0.4965479214204848 68-69 0.5090422754975745 70-71 0.5060506695917925 72-73 0.4949054711192712 74-75 0.49707585187444636 76-77 0.47243909735624157 78-79 0.45322829472597476 80-81 0.4596807780521713 82-83 0.4678050411492459 84-85 0.46933017357180146 86-87 0.4688315725875045 88-89 0.47487344333839754 90-91 0.488247681505423 92-93 0.49340966816638027 94-95 0.5005953882341899 96-97 0.49308704400007036 98-99 0.49543340157323273 100-101 0.4746974665204104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1704770.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.401662460450936 #Duplication Level Percentage of deduplicated Percentage of total 1 77.25851753066254 14.989436793247462 2 12.09896551604388 4.694800901258379 3 4.192723204542797 2.4403740121391864 4 1.9671515602481902 1.526640423219392 5 1.0681623639266202 1.0362062818930817 6 0.7107653503333305 0.8274017649450862 7 0.4728703003813604 0.6422128968899838 8 0.3279024028268524 0.5089481391693922 9 0.2481782583327575 0.43335637183752806 >10 1.3328917763843637 4.878355380542441 >50 0.1346946934531613 1.8371975752592127 >100 0.13560476201026397 5.476806625509023 >500 0.021235645057532872 2.8437887708684064 >1k 0.019415446909744342 7.993344267019739 >5k 0.0048538617274360854 6.31506392484444 >10k+ 0.006067327159295107 43.55606587135724 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 134502 7.889744657637101 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 108315 6.353643013427031 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 77126 4.524129354693009 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 50159 2.942273737806273 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 46746 2.742070777876194 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 45742 2.6831772027898193 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 36350 2.1322524446112965 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 33896 1.9883034074977854 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 24973 1.4648896918645917 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 24198 1.4194290138845709 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 20929 1.2276729412178768 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 20690 1.2136534547182318 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 20290 1.190189878986608 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 18322 1.07474908638702 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 18090 1.061140212462678 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 12404 0.7276054834376485 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 12212 0.7163429670864692 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 12163 0.7134686790593452 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11630 0.6822034643969568 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11281 0.6617314945711151 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 9950 0.5836564463241376 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 9006 0.5282824075975058 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 7903 0.46358159751755373 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 7706 0.4520257864697291 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7656 0.44909283950327605 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 7592 0.4453386673862163 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6795 0.3985874927409563 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6575 0.38568252608856324 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6025 0.3534201094575808 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 5916 0.34702628507071337 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5854 0.34338943083231166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5519 0.3237386861570769 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5311 0.3115376267766326 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 5193 0.30461587193580364 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5180 0.3038533057245259 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 5112 0.29986449785014985 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4805 0.2818562034761287 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 4574 0.2683059884911161 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4506 0.2643171806167401 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4406 0.25845128668383416 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3967 0.23270001231837728 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 3931 0.23058829050253113 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3806 0.22325592308639874 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3557 0.20864984719346302 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3266 0.19158009584870686 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 3149 0.18471699994720697 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3041 0.17838183449966857 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 2780 0.16307185133478416 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2694 0.15802718255248507 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2646 0.15521155346469023 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2596 0.1522786064982373 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2566 0.15051883831836554 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 2509 0.14717527877660916 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2501 0.1467060072619767 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2458 0.14418367287082715 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2451 0.14377306029552372 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2366 0.13878705045255374 No Hit CAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTA 2341 0.13732057696932723 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2310 0.1355021498501264 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 2269 0.133097133337635 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2265 0.13286249758031873 No Hit CTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTA 2150 0.12611671955747697 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2065 0.12113070971450693 No Hit ACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGAT 1956 0.11473688532763951 No Hit AGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACA 1908 0.11192125623984467 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1904 0.11168662048252843 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1898 0.11133466684655408 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1894 0.11110003108923784 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1894 0.11110003108923784 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1892 0.11098271321057973 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1776 0.10417827624840886 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1755 0.10294643852249863 No Hit CGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTG 1740 0.10206655443256275 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1719 0.1008347167066525 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.8658939329059054E-5 0.0 8 0.0 0.0 0.0 5.8658939329059054E-5 0.0 9 0.0 0.0 0.0 1.1731787865811811E-4 0.0 10-11 0.0 0.0 0.0 3.226241663098248E-4 0.0 12-13 0.0 0.0 0.0 4.98600984297002E-4 0.0 14-15 0.0 0.0 0.0 5.865893932905905E-4 0.0 16-17 0.0 0.0 0.0 9.97201968594004E-4 0.0 18-19 0.0 0.0 0.0 0.0016424503012136536 0.0 20-21 0.0 0.0 0.0 0.002522334391149539 0.0 22-23 0.0 0.0 0.0 0.004575397267666606 0.0 24-25 0.0 0.0 0.0 0.006980413780158027 0.0 26-27 0.0 0.0 0.0 0.009649395519630215 0.0 28-29 0.0 0.0 0.0 0.020618617174164258 0.0 30-31 0.0 0.0 0.0 0.043583591921490875 0.0 32-33 0.0 0.0 0.0 0.08379429483156085 0.0 34-35 0.0 0.0 0.0 0.1830745496459933 0.0 36-37 0.0 0.0 0.0 0.33505986144758526 0.0 38-39 0.0 0.0 0.0 0.4761346105339723 0.0 40-41 0.0 0.0 0.0 0.6275919918816029 0.0 42-43 0.0 0.0 0.0 0.7810144476967568 0.0 44-45 0.0 0.0 0.0 0.9558474163670172 0.0 46-47 0.0 0.0 0.0 1.2017163605647683 0.0 48-49 0.0 0.0 0.0 1.512814045296433 0.0 50-51 0.0 0.0 0.0 1.785548783706893 0.0 52-53 0.0 0.0 0.0 2.0020882582401147 0.0 54-55 0.0 0.0 0.0 2.227983833596321 0.0 56-57 0.0 0.0 0.0 2.456577720161664 0.0 58-59 0.0 0.0 0.0 2.8888354440775004 0.0 60-61 0.0 0.0 0.0 3.6377927814309263 0.0 62-63 0.0 0.0 0.0 4.19675381429753 0.0 64-65 0.0 0.0 0.0 4.55184570352599 0.0 66-67 0.0 0.0 0.0 4.968969421094927 0.0 68-69 0.0 0.0 0.0 5.405597235990779 0.0 70-71 0.0 0.0 0.0 5.950098840312769 0.0 72-73 0.0 0.0 0.0 6.667145714671188 0.0 74-75 0.0 0.0 0.0 7.358939915648445 0.0 76-77 0.0 0.0 0.0 7.8867530517313185 0.0 78-79 0.0 0.0 0.0 8.464455615713558 0.0 80-81 0.0 0.0 0.0 9.103926042809295 0.0 82-83 0.0 0.0 0.0 9.757738580570987 0.0 84-85 0.0 0.0 0.0 11.022601289323486 0.0 86-87 0.0 0.0 0.0 12.471154466584935 0.0 88-89 0.0 0.0 0.0 13.325697894730666 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 135 0.0 52.73455 1 GTAAGCG 115 0.0 37.220657 94-95 AGTACTC 485 0.0 32.231483 5 CGCTTCA 30 0.003929493 31.703686 46-47 GTAGCAC 65 0.005923333 29.151087 3 GTCGGGA 875 0.0 28.692999 2 GCTTATG 480 0.0 28.674412 1 CGGGAGT 870 0.0 27.768923 4 ACCACTC 1370 0.0 27.424955 94-95 GTATAGC 250 0.0 26.578213 1 TCGGGAG 1005 0.0 24.981468 3 AAGTACT 390 0.0 24.29257 4 ATGGGGT 745 0.0 24.162142 5 GGTCGGG 1070 0.0 23.952328 1 TACCTGG 605 0.0 23.489511 2 GGGAGTG 1060 0.0 23.238365 5 CTTATGT 620 0.0 22.921217 2 GTGGGTA 1080 0.0 22.796648 9 CCGTATC 115 3.5797711E-9 22.745958 94-95 ACTACCT 355 0.0 22.684473 5 >>END_MODULE