FastQCFastQC Report
Fri 27 May 2016
SRR938987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1704770
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1367368.02078872809822No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1129856.627580260093737No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT706794.145955172838565No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA504142.957231767335183No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG496802.9141761058676536No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT452162.652322600702734No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC379522.2262240654164493No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG335861.9701191363057775No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG275341.615115235486312No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT256101.5022554362172025No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA226811.3304434029223884No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG222311.304046880224312No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG215481.2639828246625644No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA194321.1398605090422755No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT189591.1121148307396305No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT132490.7771722871707034No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC126490.7419769235732679No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT122110.7162843081471402No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA119390.700329076649636No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT118580.6955777025639822No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT109860.6444271074690427No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC97370.571162092247048No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT88000.5161986660957196No Hit
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC82650.484816133554673No Hit
GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT70460.41331088651255005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69760.40920476075951595No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA69730.4090287839415288No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63200.3707244965596532No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62140.36450664899077295No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT61330.35975527490511916No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG60120.35265754324630305No Hit
CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT59700.3501938677944825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57240.335763768719534No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC54360.318869994192765No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54080.31722754389155133No Hit
TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA52950.31059908374736767No Hit
GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA49130.2881913689236671No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45770.2684819653091033No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA44370.26026971380303504No Hit
TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA43160.2531719821442189No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42240.24777535972594542No Hit
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40920.24003237973450964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38360.2250156912662705No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG35190.2064208074989588No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA33650.19738733084228374No Hit
TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG32290.18940971509353166No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31430.1843650463112326No Hit
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA29490.17298521208139517No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28850.16923103996433536No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28580.16764724860245078No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27970.16406905330337815No Hit
CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG27600.161898672548203No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA27110.1590243845210791No Hit
CAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTA26980.15826181830980132No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT26440.15509423558603214No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG25830.15151604028695953No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC25790.1512814045296433No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC25060.146999301958622No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA24750.14518087483942116No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA23470.1376725306053016No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23380.13714460015134006No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG22600.13256920288367344No Hit
CTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTA21030.12335974940901118No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG20750.12171729910779754No Hit
ACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGAT20430.11984021304926765No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC20010.11737653759744716No Hit
AGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACA19890.11667263032549846No Hit
CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG19520.11450224957032326No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA18830.1104547827566182No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA18770.11010282912064386No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG18740.10992685230265666No Hit
CGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTG18610.10916428609137889No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG17070.1001308094347038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACT1650.040.423271
GCTTATG4250.040.355331
GTAAGCG1250.039.86527394-95
CTCCGAC2400.035.5939994-95
CACGTAC550.002542555334.6485181
ACCACTC13950.033.6803494-95
CTTATGT5200.031.9423892
CCACTGT16200.031.63910794-95
GTATGCG300.003968913631.63910394-95
TATGTAC5700.029.9730114
TTATGTA5650.029.3983083
GAGACGA2000.027.28872994-95
CACGAGC1350.026.36592194-95
ACGAGAG906.7702786E-926.36592194-95
CGGGAGT7500.025.9433064
AACGACG559.5382835E-525.88654194-95
GCGTTGT4600.025.7927594-95
GTCGGGA7600.025.6019462
GCTTGTC7300.025.35462694-95
ATACCAT5900.025.33809994-95
Produced by FastQC (version 0.11.5)