##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938987_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1704770 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.019819682420504 33.0 31.0 34.0 30.0 34.0 2 32.23356523167348 33.0 31.0 34.0 30.0 34.0 3 32.34829566451779 34.0 31.0 34.0 30.0 34.0 4 35.763371011925365 37.0 35.0 37.0 35.0 37.0 5 35.714049989148094 37.0 35.0 37.0 33.0 37.0 6 35.83357696346135 37.0 35.0 37.0 35.0 37.0 7 35.80862403726016 37.0 35.0 37.0 33.0 37.0 8 35.846123523994436 37.0 35.0 37.0 35.0 37.0 9 37.40600843515548 39.0 37.0 39.0 34.0 39.0 10-11 37.30527314535098 39.0 37.0 39.0 33.5 39.0 12-13 37.21215325234489 39.0 37.0 39.0 33.5 39.0 14-15 38.04732104624084 40.0 37.0 41.0 33.0 41.0 16-17 38.216735688685276 40.0 37.5 41.0 33.5 41.0 18-19 38.58972207394546 40.0 38.0 41.0 34.0 41.0 20-21 38.57156889199129 40.0 38.0 41.0 34.0 41.0 22-23 38.61943194683154 40.0 38.0 41.0 34.0 41.0 24-25 38.48986578834682 40.0 38.0 41.0 34.0 41.0 26-27 37.910643664541254 39.5 37.0 41.0 32.5 41.0 28-29 37.636491726156606 39.0 36.5 41.0 32.5 41.0 30-31 37.698438205740366 39.5 37.0 41.0 33.0 41.0 32-33 37.7979888782651 40.0 37.0 41.0 33.0 41.0 34-35 37.8275221877438 40.0 37.0 41.0 33.0 41.0 36-37 37.51548449350939 40.0 36.5 41.0 33.0 41.0 38-39 37.09055004487409 39.5 35.5 41.0 31.0 41.0 40-41 36.76747420473143 39.0 35.0 40.5 30.5 41.0 42-43 36.938487889861975 39.0 36.0 41.0 31.0 41.0 44-45 36.929227696404794 39.0 36.0 41.0 31.0 41.0 46-47 36.60088076397403 39.0 35.5 41.0 30.0 41.0 48-49 36.32490541246033 39.0 35.0 41.0 30.0 41.0 50-51 36.14277439185345 39.0 35.0 40.0 29.0 41.0 52-53 35.32402112894995 38.0 34.0 40.0 27.5 41.0 54-55 35.153757984948115 38.0 34.0 40.0 27.5 41.0 56-57 35.39127389618541 38.0 34.0 40.0 28.0 41.0 58-59 35.32624488933991 38.0 34.5 40.0 28.0 41.0 60-61 34.88033576376872 38.0 34.0 40.0 27.0 41.0 62-63 34.14014559148741 37.0 33.5 39.0 25.5 41.0 64-65 33.210743384738116 36.0 32.0 39.0 22.0 40.0 66-67 32.76527009508614 36.0 31.5 39.0 22.0 40.0 68-69 32.482543686245066 35.0 31.0 38.0 22.0 39.0 70-71 31.811357837127588 35.0 31.0 37.0 21.5 39.0 72-73 31.07899482041566 34.5 30.5 36.5 20.0 38.0 74-75 30.542657073974787 34.0 30.0 36.0 18.0 37.0 76-77 28.34082632847833 31.5 26.0 34.0 11.5 35.5 78-79 29.147974213530272 33.0 29.0 35.0 11.5 36.0 80-81 29.445048598931233 34.0 29.0 35.0 9.0 36.0 82-83 29.18182804718525 33.5 29.0 35.0 7.0 35.5 84-85 28.879570264610475 34.0 29.0 35.0 2.0 35.0 86-87 28.0281867935264 33.0 27.0 35.0 2.0 35.0 88-89 26.98887327909337 32.0 25.5 34.0 2.0 35.0 90-91 26.08130187649947 31.0 24.5 34.0 2.0 35.0 92-93 25.962050305906367 31.5 24.0 34.0 2.0 35.0 94-95 25.548430286783553 32.0 23.5 34.0 2.0 35.0 96-97 24.320071329270224 31.0 16.5 34.0 2.0 35.0 98-99 22.949768883779043 31.0 2.0 34.0 2.0 35.0 100-101 17.778105844190126 23.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04703384488659168 1101 2 0.13267113426596921 1101 3 0.15725135321895323 1101 4 0.11484689542203341 1101 5 0.169641971179459 1101 6 0.16496637619718513 1101 7 0.15819070528773693 1101 8 0.140204282415354 1101 9 0.27632070905191597 1101 10-11 0.2970966133281294 1101 12-13 0.27269132657398387 1101 14-15 0.19733353008486887 1101 16-17 0.28579324126873473 1101 18-19 0.2837670741299334 1101 20-21 0.32707705560253686 1101 22-23 0.29831969156393967 1101 24-25 0.2893997656990308 1101 26-27 0.1696898017470616 1101 28-29 0.24759218104888703 1101 30-31 0.2376849252757296 1101 32-33 0.36022187736184463 1101 34-35 0.3360393916581401 1101 36-37 0.36453122951748895 1101 38-39 0.4155653040347502 1101 40-41 0.33198187945242097 1101 42-43 0.24633024007101056 1101 44-45 0.33396074324139136 1101 46-47 0.380185950966343 1101 48-49 0.283805079535675 1101 50-51 0.18255284475177547 1101 52-53 0.19855883310941636 1101 54-55 0.430332208071313 1101 56-57 0.33144893268713815 1101 58-59 0.5054073515435675 1101 60-61 0.524729091532727 1101 62-63 0.5274877821476238 1101 64-65 0.4110903743456973 1101 66-67 0.4719955907839335 1101 68-69 0.48222791567846457 1101 70-71 0.5600204273843197 1101 72-73 0.7073889584972299 1101 74-75 0.623035615281271 1101 76-77 0.4185369567366344 1101 78-79 0.8424835443273757 1101 80-81 0.7432130242512542 1101 82-83 0.7852613009907579 1101 84-85 0.9369449093372388 1101 86-87 1.2361327670873195 1101 88-89 1.245518991723742 1101 90-91 1.174270731155211 1101 92-93 1.2837497606000383 1101 94-95 1.2824877806553836 1101 96-97 1.5510815079466909 1101 98-99 1.654046038560665 1101 100-101 1.8818782203191091 1102 1 0.11183920524064916 1102 2 0.135713242507137 1102 3 0.1878451903076339 1102 4 0.11698905341654608 1102 5 0.03732205065474403 1102 6 0.12274675907032417 1102 7 0.04978043853825653 1102 8 0.10882024511009547 1102 9 0.21198036364011585 1102 10-11 0.24127388645448633 1102 12-13 0.25097748636353145 1102 14-15 0.35945322590657014 1102 16-17 0.24720065939520453 1102 18-19 0.2549278148369396 1102 20-21 0.19886605425952553 1102 22-23 0.20385682447041376 1102 24-25 0.22016273977850886 1102 26-27 0.26989056300578795 1102 28-29 0.35939534965420705 1102 30-31 0.40196324159646224 1102 32-33 0.2864340867681605 1102 34-35 0.39392541909727896 1102 36-37 0.4421529544952918 1102 38-39 0.4976186945790104 1102 40-41 0.5537436031945262 1102 42-43 0.4492604845778132 1102 44-45 0.46044074272109725 1102 46-47 0.4769980784259644 1102 48-49 0.5508399101304136 1102 50-51 0.38645823859992134 1102 52-53 0.3049279525402113 1102 54-55 0.5655316442000498 1102 56-57 0.45748363158183025 1102 58-59 0.3835905088974698 1102 60-61 0.3517571501441168 1102 62-63 0.48797269029903845 1102 64-65 0.4054610107657979 1102 66-67 0.3715698419635416 1102 68-69 0.33054239910539707 1102 70-71 0.42750824106616747 1102 72-73 0.44275501143088647 1102 74-75 0.5508613533705464 1102 76-77 0.2874988759150874 1102 78-79 0.5896906093026466 1102 80-81 0.7636503090192512 1102 82-83 0.6866950022170322 1102 84-85 0.900946289748731 1102 86-87 0.9849885214033414 1102 88-89 0.699118508173239 1102 90-91 0.9044724354512361 1102 92-93 1.1104587311610175 1102 94-95 1.2445215869165018 1102 96-97 1.2878655719171412 1102 98-99 1.4214497512009423 1102 100-101 1.8370703316495387 1103 1 0.11849202410756732 1103 2 0.07982441335175139 1103 3 0.07542088106120559 1103 4 0.09459972046093412 1103 5 0.1612593218924161 1103 6 0.10083225380859062 1103 7 0.16588144324146015 1103 8 0.1257506691714667 1103 9 0.10152860130505559 1103 10-11 0.0430232643497348 1103 12-13 0.12971916883095247 1103 14-15 0.19397919495745697 1103 16-17 0.08869895001551242 1103 18-19 0.0891183865294849 1103 20-21 0.20378132568391294 1103 22-23 0.21950078739844514 1103 24-25 0.17996777069864578 1103 26-27 0.12697145111062724 1103 28-29 0.14116856550926116 1103 30-31 0.23366418706692116 1103 32-33 0.33446491998613936 1103 34-35 0.38720097367993134 1103 36-37 0.2683762796412026 1103 38-39 0.3215801498373665 1103 40-41 0.39616861261060166 1103 42-43 0.38295562588331933 1103 44-45 0.3715041072696508 1103 46-47 0.42721360191142566 1103 48-49 0.3469348145049409 1103 50-51 0.5872747410352233 1103 52-53 0.6535766251151287 1103 54-55 0.5969887777215988 1103 56-57 0.8232687551497051 1103 58-59 0.7185144479392562 1103 60-61 0.4785878523418461 1103 62-63 0.8123256772066654 1103 64-65 0.6730920068738229 1103 66-67 0.5439065414764812 1103 68-69 0.4811390321746458 1103 70-71 0.634418432639027 1103 72-73 0.6975061051441678 1103 74-75 0.6927225652595865 1103 76-77 0.573533066728217 1103 78-79 0.7115688184135429 1103 80-81 0.4692623699645928 1103 82-83 0.6205959692559411 1103 84-85 0.5998469831581197 1103 86-87 0.6854032310824216 1103 88-89 0.6303556113701951 1103 90-91 0.6851786973822911 1103 92-93 0.721401215845809 1103 94-95 0.950148737724998 1103 96-97 0.9175840715260115 1103 98-99 1.233749760214618 1103 100-101 1.245702417446882 1104 1 -0.009298587350215826 1104 2 0.10156127496919964 1104 3 0.12679017327553055 1104 4 0.07088224903685614 1104 5 0.09451908007577003 1104 6 0.21441785073307784 1104 7 0.13746128915422418 1104 8 0.1339900994287646 1104 9 0.09959433293904851 1104 10-11 0.17304838997787186 1104 12-13 0.19720574149589964 1104 14-15 0.2713964657503567 1104 16-17 0.2621516521498677 1104 18-19 0.21324296166804402 1104 20-21 0.2844890334727026 1104 22-23 0.22879672461709077 1104 24-25 0.26744989614298476 1104 26-27 0.19759658546187353 1104 28-29 0.172042868829962 1104 30-31 0.09223244684068277 1104 32-33 0.12373753850339853 1104 34-35 0.17264516612386416 1104 36-37 0.29279335104907744 1104 38-39 0.3858419034286342 1104 40-41 0.22574952242937485 1104 42-43 0.1681677217002786 1104 44-45 0.08415271141873149 1104 46-47 0.1921480073546391 1104 48-49 -0.06726459232461934 1104 50-51 -2.1069451200617095E-4 1104 52-53 0.08134200190608709 1104 54-55 0.29980053489556013 1104 56-57 0.16718416293880267 1104 58-59 0.10031437285833533 1104 60-61 -0.04547795873461524 1104 62-63 -0.06964909903037864 1104 64-65 0.0969608084140745 1104 66-67 0.019765376881032637 1104 68-69 0.004521793781812278 1104 70-71 0.034498887610720175 1104 72-73 0.10472158427572253 1104 74-75 0.18396013874387052 1104 76-77 -0.023481376224303574 1104 78-79 0.2126245465271417 1104 80-81 0.29194638832047914 1104 82-83 0.23545322983035533 1104 84-85 0.3252967287864479 1104 86-87 0.4500132464412978 1104 88-89 0.3676290445621966 1104 90-91 0.7221831165643735 1104 92-93 0.6338125829728298 1104 94-95 0.9223436786416066 1104 96-97 0.5342831122971319 1104 98-99 0.5823189779711022 1104 100-101 1.1975826727220173 1105 1 0.04597277048376469 1105 2 0.039925196597593526 1105 3 0.054147927216874336 1105 4 0.04337837475038242 1105 5 0.10671206668403244 1105 6 0.055579609457097945 1105 7 -0.033905511395161625 1105 8 0.10046361939745907 1105 9 0.08100660897219569 1105 10-11 0.16528923417401842 1105 12-13 0.14187550451308795 1105 14-15 0.18181896020205812 1105 16-17 0.06878540806616229 1105 18-19 0.12302355794834341 1105 20-21 0.10681254322413025 1105 22-23 0.01758220940606492 1105 24-25 -0.0891326195206048 1105 26-27 0.06118706674310914 1105 28-29 0.22272704069088434 1105 30-31 0.02931141763315992 1105 32-33 0.05943130997456336 1105 34-35 0.12984849121796316 1105 36-37 -0.025221013154244076 1105 38-39 0.1720111144057057 1105 40-41 0.18544963129807712 1105 42-43 0.21024110667024587 1105 44-45 0.12641947398529396 1105 46-47 0.052570367115471583 1105 48-49 -0.009778531866892592 1105 50-51 -0.06265183941513186 1105 52-53 -0.1434224141532212 1105 54-55 0.14462473060832082 1105 56-57 -0.003680625521504055 1105 58-59 -0.0865453404502361 1105 60-61 -0.10240428244755861 1105 62-63 -0.09215190156240993 1105 64-65 -0.29333502274977974 1105 66-67 -0.08969442424599094 1105 68-69 0.05732984814151365 1105 70-71 0.04304560013385483 1105 72-73 0.03330560231967894 1105 74-75 -0.10215615796547795 1105 76-77 -0.16518886384332632 1105 78-79 0.1687339893957045 1105 80-81 0.2506994280046406 1105 82-83 0.11001485917418563 1105 84-85 0.16731050342862375 1105 86-87 0.34605479741813383 1105 88-89 0.4512955453666869 1105 90-91 0.49510374939592694 1105 92-93 0.7369984836871879 1105 94-95 0.6004800419805996 1105 96-97 0.5096291042626291 1105 98-99 0.8652150085543937 1105 100-101 0.6315400865496876 1106 1 0.02257093248822173 1106 2 3.2493037731029517E-4 1106 3 -0.010301805778524908 1106 4 0.03812903974084492 1106 5 0.07752083402588994 1106 6 0.08641903246722649 1106 7 0.19628315093758175 1106 8 0.0962484650098645 1106 9 -0.02366188179637163 1106 10-11 0.1393540458142084 1106 12-13 0.056927034271275545 1106 14-15 -0.02608888007474519 1106 16-17 -0.05414258920889381 1106 18-19 0.015237399161840415 1106 20-21 0.08250481678562949 1106 22-23 -0.004638108041447708 1106 24-25 0.06355916328129041 1106 26-27 0.07486222870497983 1106 28-29 0.039684913797032095 1106 30-31 0.02451978878187333 1106 32-33 0.11735046685405592 1106 34-35 0.18357378236837718 1106 36-37 0.1453011416165495 1106 38-39 0.1406820522495451 1106 40-41 0.2847847528787284 1106 42-43 0.5199333847135748 1106 44-45 0.49495416441458673 1106 46-47 0.4958842717467604 1106 48-49 0.4640554658966778 1106 50-51 0.49258158512329686 1106 52-53 0.576431724922223 1106 54-55 0.6419854971792915 1106 56-57 0.5559914843190157 1106 58-59 0.6037797485699699 1106 60-61 0.6588562974555074 1106 62-63 0.641797570366812 1106 64-65 0.5644886713030743 1106 66-67 0.6769998056311906 1106 68-69 0.5515690250995604 1106 70-71 0.6696767035362292 1106 72-73 0.8020760366307229 1106 74-75 0.8705529022635012 1106 76-77 0.6234041126920893 1106 78-79 0.859488773181365 1106 80-81 0.8761803836189053 1106 82-83 0.509815306011923 1106 84-85 0.6135863327339166 1106 86-87 1.0402286518081922 1106 88-89 0.9751651736218889 1106 90-91 1.2272728171991822 1106 92-93 1.328640556350983 1106 94-95 1.3417460897075308 1106 96-97 1.4315944059901469 1106 98-99 1.3500597245973047 1106 100-101 1.8981951004769506 1107 1 0.00909636158442595 1107 2 6.185755503054224E-4 1107 3 0.001961478786675741 1107 4 0.06436781932847424 1107 5 0.03144157286017446 1107 6 0.022855648292974706 1107 7 0.033466978573628126 1107 8 0.09590947756751689 1107 9 -0.007539466305722442 1107 10-11 -0.017741083706333427 1107 12-13 0.06804416702768634 1107 14-15 0.14168723275410144 1107 16-17 0.12033590272162087 1107 18-19 0.09579801673795174 1107 20-21 0.16725599568463423 1107 22-23 0.12195714814956915 1107 24-25 0.02516865550545333 1107 26-27 0.04795195169970157 1107 28-29 -0.056270483804283344 1107 30-31 -0.04754599061574538 1107 32-33 0.16642375723458258 1107 34-35 0.21384766406967515 1107 36-37 0.18422496084627227 1107 38-39 0.08946112168223408 1107 40-41 0.06829268993502069 1107 42-43 0.04031689394611959 1107 44-45 0.08492618175692002 1107 46-47 0.08096832048034486 1107 48-49 0.11276155586725878 1107 50-51 0.018918606294533902 1107 52-53 -0.009078757058901488 1107 54-55 -0.053278106940943815 1107 56-57 0.09093633948285174 1107 58-59 -0.05763153316242153 1107 60-61 0.016211677207806474 1107 62-63 0.1879372878916783 1107 64-65 0.14502553816664943 1107 66-67 0.3233074334963888 1107 68-69 0.2511268300155294 1107 70-71 0.3896554430499286 1107 72-73 0.10039074364769007 1107 74-75 0.17157015247797602 1107 76-77 0.1730362645354333 1107 78-79 0.5141357800158133 1107 80-81 0.432341822249942 1107 82-83 0.3032737120368303 1107 84-85 0.4064668047541389 1107 86-87 0.46994345613110866 1107 88-89 0.8519540341058836 1107 90-91 0.8911923181175432 1107 92-93 1.0017202752577035 1107 94-95 1.1867924827659913 1107 96-97 1.1987085936581998 1107 98-99 0.9374371565263253 1107 100-101 0.8945867914701076 1108 1 0.07698847013295307 1108 2 0.0964885705923848 1108 3 0.14324047215023228 1108 4 0.09479106419583871 1108 5 -0.019690684067469988 1108 6 0.0500069663224707 1108 7 0.05232050496707075 1108 8 0.10149236140291151 1108 9 0.26176878474409904 1108 10-11 0.13247410783055358 1108 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-1.5567437638126904 2316 88-89 -1.639576639168208 2316 90-91 -1.8899402006794475 2316 92-93 -1.7391268710170777 2316 94-95 -1.6220525464737783 2316 96-97 -1.2926773048000655 2316 98-99 -1.6904832600454114 2316 100-101 -1.0919693241978514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 16.0 5 73.0 6 155.0 7 273.0 8 575.0 9 964.0 10 1591.0 11 2479.0 12 3835.0 13 5469.0 14 6628.0 15 8514.0 16 10955.0 17 14326.0 18 14609.0 19 11259.0 20 11413.0 21 12467.0 22 13931.0 23 15383.0 24 18039.0 25 21339.0 26 24992.0 27 30007.0 28 36461.0 29 44431.0 30 53948.0 31 65169.0 32 80972.0 33 103877.0 34 141513.0 35 204625.0 36 297646.0 37 315097.0 38 127543.0 39 4189.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.665083929803735 14.825145260857175 12.648587666428785 18.861183142910303 2 12.036521055626272 24.08330742563513 39.583169577127705 24.29700194161089 3 29.303953025921388 28.78001137983423 29.26359567566299 12.652439918581393 4 11.880194982314329 13.013251054394434 40.692116825143565 34.41443713814767 5 12.059456700903933 42.86273221607607 32.71104019897112 12.366770884048876 6 24.175284642503094 35.99881508942555 18.86342439156015 20.9624758765112 7 24.63681317714413 39.33351713134323 17.66291053925163 18.366759152261007 8 18.044838893223133 29.24634994750025 22.003437413844683 30.705373745431935 9 24.162555652668686 26.70712178182394 22.096294514802583 27.03402805070479 10-11 21.85241997454202 22.072156361268675 27.643259794576398 28.432163869612907 12-13 37.58222516820451 19.63258973351244 23.17212879156719 19.613056306715862 14-15 31.39621576786852 21.938099131958904 30.324098787041915 16.341586313130662 16-17 18.90043062512502 26.324006544140982 34.49189344242581 20.283669388308184 18-19 17.53935523494356 34.9044631195995 30.427030659145377 17.12915098631157 20-21 20.340104530229887 30.866480522299195 23.246918939211742 25.54649600825918 22-23 18.80065932647806 28.177144130879828 20.54681863242549 32.47537791021663 24-25 23.160279685822722 28.000492735090365 25.859529437988705 22.97969814109821 26-27 29.15661032180597 20.8010819057943 29.400426274637127 20.6418814977626 28-29 21.55158760419294 22.621966599599947 34.245763358106956 21.580682438100155 30-31 19.17235169553664 34.15446071904128 28.70140840113329 17.97177918428879 32-33 18.978718536811417 31.50847328378608 25.046252573660965 24.466555605741537 34-35 22.384315602793194 24.17878260420645 25.68622757722902 27.750674215771337 36-37 26.879611737637767 24.46640767254679 29.340398124986617 19.313582464828833 38-39 31.559676030327278 17.872978122778076 32.28550443387438 18.281841413020267 40-41 21.44572376380939 23.667163826695 36.202371995958195 18.68474041353742 42-43 17.78820517663843 31.216046018735295 30.57244190609827 20.423306898528008 44-45 19.572780679808417 33.114190098812436 23.665474895072283 23.647554326306867 46-47 19.41577460195113 27.91929388011049 20.148608038818526 32.51632347911985 48-49 23.91362562206018 25.326146222968777 24.40133077689953 26.35889737807151 50-51 30.052705366151244 27.915425045167648 24.688020601140337 17.34384898754077 52-53 21.22083917478604 27.55984678285047 32.12368823946925 19.09562580289423 54-55 15.591546073663896 26.287769024560497 34.29811646145814 23.82256844031746 56-57 22.596479290461467 33.254016670870556 22.49183174269843 21.657672295969544 58-59 19.567820722678412 28.069127801611433 25.580471803727868 26.782579671982287 60-61 20.562176601951375 22.49275406526122 29.232602570818344 27.71246676196906 62-63 32.58440543704994 20.90408670372395 25.352046167527398 21.159461691698713 64-65 28.153040128041823 23.26996451983877 30.550187402240383 18.02680794987903 66-67 19.463848850650063 25.41915130568018 33.23322918160677 21.88377066206299 68-69 18.310513506236468 33.253692224755696 29.141958467347635 19.293835801660205 70-71 19.985781068936298 31.081914593145875 22.847223627593056 26.08508071032477 72-73 19.378039266293985 27.646662013057483 20.922793104055092 32.05250561659344 74-75 22.260392897575628 28.420930682731395 25.193515840846565 24.125160578846412 76-77 27.086129379591828 22.161036999984734 28.138029869112746 22.614803751310692 78-79 21.407371754542343 22.48852370447868 32.83937820490823 23.26472633607075 80-81 19.31398836321914 32.7883810514868 28.098883894397297 19.798746690896763 82-83 19.382033758816075 30.605985149311977 24.411657998339365 25.600323093532584 84-85 21.403371077505337 24.610467181282598 24.593749349251055 29.39241239196101 86-87 24.954953791308505 24.973359769053417 27.548347248878763 22.523339190759316 88-89 28.811331380012746 19.293900526736813 30.150822739102107 21.743945354148334 90-91 21.093977228703277 23.253411631990033 33.64957190480284 22.00303923450385 92-93 18.17652821201687 29.514186664476732 29.986684420772303 22.322600702734093 94-95 19.10847117800367 31.838437970924886 24.584324921381757 24.468765929689688 96-97 18.249534614503602 27.660191011160162 21.684837744926806 32.40543662940943 98-99 22.324301811974635 25.67120491327276 23.93387377769435 28.070619497058253 100-101 27.15816049395751 26.56799013586647 25.222435741763366 21.051413628412647 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6665.0 1 4196.5 2 1776.0 3 1662.5 4 1223.0 5 1187.0 6 2000.0 7 3485.5 8 4683.0 9 4567.5 10 3293.0 11 2206.0 12 1974.5 13 1928.0 14 1834.5 15 1699.0 16 1593.0 17 1581.5 18 1631.0 19 1724.0 20 1906.5 21 2122.5 22 2403.5 23 2810.0 24 3341.0 25 3872.0 26 4632.5 27 5511.0 28 6191.0 29 6939.5 30 7842.0 31 8908.5 32 10261.5 33 11717.0 34 13310.5 35 14422.0 36 15490.5 37 16909.0 38 16994.5 39 16264.0 40 16037.5 41 16197.0 42 16645.0 43 17598.0 44 21669.0 45 27630.0 46 50597.0 47 119882.0 48 425443.0 49 428506.5 50 138478.5 51 69951.5 52 26644.0 53 13116.0 54 9822.5 55 8419.5 56 7738.0 57 7476.5 58 9018.0 59 9899.5 60 10316.0 61 10758.5 62 8933.5 63 5301.5 64 2341.5 65 1635.5 66 1095.0 67 695.0 68 628.5 69 555.0 70 431.0 71 376.0 72 314.5 73 338.5 74 291.0 75 242.5 76 193.5 77 75.0 78 25.5 79 13.0 80 6.0 81 3.0 82 1.5 83 1.0 84 0.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37811552291511463 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.039072719487086E-4 16-17 0.002287698633833303 18-19 5.57259923626061E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 2.9329469664529527E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011555811047824634 36-37 0.020853252931480493 38-39 0.009737383928623803 40-41 0.005132657191292667 42-43 0.004282102571021311 44-45 0.0014664734832264764 46-47 9.97201968594004E-4 48-49 0.0030209353754465412 50-51 5.865893932905905E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.505546202713562E-4 60-61 0.00123183772591024 62-63 0.0012904966652392991 64-65 8.505546202713562E-4 66-67 0.002668981739472187 68-69 0.002287698633833303 70-71 2.9329469664529527E-5 72-73 0.0 74-75 0.0 76-77 0.08634595869237492 78-79 0.0035488658294080726 80-81 1.7597681798717715E-4 82-83 2.9329469664529527E-5 84-85 2.053062876517067E-4 86-87 0.0891029288408407 88-89 0.41929409832411413 90-91 0.370489860802337 92-93 0.0 94-95 7.625662112777676E-4 96-97 2.9329469664529527E-5 98-99 0.0 100-101 4.6927151463247243E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1704770.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.87390289356322 #Duplication Level Percentage of deduplicated Percentage of total 1 75.30521055228824 13.459980207909322 2 12.974065922088906 4.637943888522097 3 4.666816400361692 2.5024266948645946 4 2.196911241990843 1.570695128204868 5 1.2245617838659046 1.0943849205993865 6 0.7508630280797703 0.805251171015879 7 0.5001431228101384 0.6257656726994315 8 0.356789921618639 0.5101782729930868 9 0.2535271847845657 0.4078368253546007 >10 1.4219329624263641 4.794245639977004 >50 0.14256181190057843 1.7771780363900778 >100 0.15011692047290615 5.626095974592361 >500 0.02266535560750638 2.798369785659331 >1k 0.0220083887783033 8.400863212019631 >5k 0.004927251219023127 5.889422911957152 >10k+ 0.006898151706632377 45.0993616572412 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 136736 8.02078872809822 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 112985 6.627580260093737 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 70679 4.145955172838565 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 50414 2.957231767335183 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 49680 2.9141761058676536 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 45216 2.652322600702734 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 37952 2.2262240654164493 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 33586 1.9701191363057775 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 27534 1.615115235486312 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 25610 1.5022554362172025 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 22681 1.3304434029223884 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 22231 1.304046880224312 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 21548 1.2639828246625644 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 19432 1.1398605090422755 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 18959 1.1121148307396305 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 13249 0.7771722871707034 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 12649 0.7419769235732679 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12211 0.7162843081471402 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 11939 0.700329076649636 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11858 0.6955777025639822 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 10986 0.6444271074690427 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 9737 0.571162092247048 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 8800 0.5161986660957196 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 8265 0.484816133554673 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 7046 0.41331088651255005 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6976 0.40920476075951595 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6973 0.4090287839415288 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6320 0.3707244965596532 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6214 0.36450664899077295 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6133 0.35975527490511916 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 6012 0.35265754324630305 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 5970 0.3501938677944825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5724 0.335763768719534 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5436 0.318869994192765 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5408 0.31722754389155133 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 5295 0.31059908374736767 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 4913 0.2881913689236671 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4577 0.2684819653091033 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4437 0.26026971380303504 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 4316 0.2531719821442189 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4224 0.24777535972594542 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4092 0.24003237973450964 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3836 0.2250156912662705 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3519 0.2064208074989588 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3365 0.19738733084228374 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 3229 0.18940971509353166 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3143 0.1843650463112326 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 2949 0.17298521208139517 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2885 0.16923103996433536 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2858 0.16764724860245078 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2797 0.16406905330337815 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2760 0.161898672548203 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 2711 0.1590243845210791 No Hit CAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTA 2698 0.15826181830980132 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2644 0.15509423558603214 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2583 0.15151604028695953 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2579 0.1512814045296433 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2506 0.146999301958622 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 2475 0.14518087483942116 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2347 0.1376725306053016 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2338 0.13714460015134006 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2260 0.13256920288367344 No Hit CTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTA 2103 0.12335974940901118 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 2075 0.12171729910779754 No Hit ACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGAT 2043 0.11984021304926765 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2001 0.11737653759744716 No Hit AGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACA 1989 0.11667263032549846 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1952 0.11450224957032326 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1883 0.1104547827566182 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1877 0.11010282912064386 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1874 0.10992685230265666 No Hit CGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTG 1861 0.10916428609137889 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1707 0.1001308094347038 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.8658939329059054E-5 0.0 5 0.0 0.0 0.0 5.8658939329059054E-5 0.0 6 0.0 0.0 0.0 5.8658939329059054E-5 0.0 7 0.0 0.0 0.0 1.1731787865811811E-4 0.0 8 0.0 0.0 0.0 1.1731787865811811E-4 0.0 9 0.0 0.0 0.0 1.7597681798717715E-4 0.0 10-11 0.0 0.0 0.0 3.8128310563888387E-4 0.0 12-13 0.0 0.0 0.0 5.57259923626061E-4 0.0 14-15 0.0 0.0 0.0 6.452483326196496E-4 0.0 16-17 0.0 0.0 0.0 0.0010851903775875925 0.0 18-19 0.0 0.0 0.0 0.0017597681798717717 0.0 20-21 0.0 0.0 0.0 0.002610322800143128 0.0 22-23 0.0 0.0 0.0 0.004604726737331135 0.0 24-25 0.0 0.0 0.0 0.007068402189151616 0.0 26-27 0.0 0.0 0.0 0.00979604286795286 0.0 28-29 0.0 0.0 0.0 0.020853252931480493 0.0 30-31 0.0 0.0 0.0 0.04422884025411053 0.0 32-33 0.0 0.0 0.0 0.0853194272541164 0.0 34-35 0.0 0.0 0.0 0.18583151979445908 0.0 36-37 0.0 0.0 0.0 0.3399285534118972 0.0 38-39 0.0 0.0 0.0 0.48355496635909834 0.0 40-41 0.0 0.0 0.0 0.6377693178551946 0.0 42-43 0.0 0.0 0.0 0.793215507077201 0.0 44-45 0.0 0.0 0.0 0.9698082439273332 0.0 46-47 0.0 0.0 0.0 1.2184634877432146 0.0 48-49 0.0 0.0 0.0 1.531320940654751 0.0 50-51 0.0 0.0 0.0 1.8055808114877667 0.0 52-53 0.0 0.0 0.0 2.0233521237468985 0.0 54-55 0.0 0.0 0.0 2.25050886629868 0.0 56-57 0.0 0.0 0.0 2.4803639200595975 0.0 58-59 0.0 0.0 0.0 2.916023862456519 0.0 60-61 0.0 0.0 0.0 3.6706124579855346 0.0 62-63 0.0 0.0 0.0 4.234823465922089 0.0 64-65 0.0 0.0 0.0 4.592789643177673 0.0 66-67 0.0 0.0 0.0 5.014019486499645 0.0 68-69 0.0 0.0 0.0 5.4546654387395375 0.0 70-71 0.0 0.0 0.0 6.002627920481942 0.0 72-73 0.0 0.0 0.0 6.727183139074479 0.0 74-75 0.0 0.0 0.0 7.425781777013908 0.0 76-77 0.0 0.0 0.0 7.957525062031829 0.0 78-79 0.0 0.0 0.0 8.535638238589371 0.0 80-81 0.0 0.0 0.0 9.177367034849276 0.0 82-83 0.0 0.0 0.0 9.836194911923602 0.0 84-85 0.0 0.0 0.0 11.10278805938631 0.0 86-87 0.0 0.0 0.0 12.552221120737695 0.0 88-89 0.0 0.0 0.0 13.409022918047597 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 165 0.0 40.42327 1 GCTTATG 425 0.0 40.35533 1 GTAAGCG 125 0.0 39.865273 94-95 CTCCGAC 240 0.0 35.59399 94-95 CACGTAC 55 0.0025425553 34.648518 1 ACCACTC 1395 0.0 33.68034 94-95 CTTATGT 520 0.0 31.942389 2 CCACTGT 1620 0.0 31.639107 94-95 GTATGCG 30 0.0039689136 31.639103 94-95 TATGTAC 570 0.0 29.973011 4 TTATGTA 565 0.0 29.398308 3 GAGACGA 200 0.0 27.288729 94-95 CACGAGC 135 0.0 26.365921 94-95 ACGAGAG 90 6.7702786E-9 26.365921 94-95 CGGGAGT 750 0.0 25.943306 4 AACGACG 55 9.5382835E-5 25.886541 94-95 GCGTTGT 460 0.0 25.79275 94-95 GTCGGGA 760 0.0 25.601946 2 GCTTGTC 730 0.0 25.354626 94-95 ATACCAT 590 0.0 25.338099 94-95 >>END_MODULE