FastQCFastQC Report
Fri 27 May 2016
SRR938986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1717165
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1352667.877286108207423No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA1096296.384302032710893No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT771764.494384639798739No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA498832.904962540000524No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG471642.746620155896492No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT452922.637603258859807No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC363242.1153470982695315No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG339421.9766300850529797No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG249331.451986268063931No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT243111.4157637734288786No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA212431.2370971921743106No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG210831.2277795086669014No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG205361.195924678175947No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT183501.0686218272559713No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA181831.0588964950951132No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC123770.7207810548200085No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA121880.7097745411768817No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT121480.7074451203000295No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT116870.6805985446943071No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT113080.6585272818861321No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT101920.5935364394219542No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC92310.5375721028555788No Hit
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC78340.45621707873151385No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT78170.45522707485885167No Hit
GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT75590.4402023102031546No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74630.43461170009870925No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA69120.40252392752006944No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66540.38749916286437236No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT61300.3569837493776079No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59520.34661782647561534No Hit
CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT58590.34120192293693385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55530.3233818532290141No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54760.3188977180410735No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG52090.30334883368808474No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC51270.29857352089053757No Hit
TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA50510.2941476212245183No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48060.2798799183537983No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC44910.26153572894858673No Hit
GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA42820.24936450486703374No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA42130.2453462538544636No Hit
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40450.23556268617168416No Hit
TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA39320.22898207219457656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39290.2288073656288126No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG35290.2055131568602901No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32030.1865283767139442No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31850.1854801373193607No Hit
TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG29630.17255185145283067No Hit
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA27810.16195298646315293No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT27210.15845885514787456No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT26690.15543060800796663No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25750.14995646894736384No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25690.14960705581583597No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC25210.1468117507636133No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA25130.14634586658824283No Hit
CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG25060.1459382179347937No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC24900.1450064495840528No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23440.13650406338354207No Hit
CAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTA23430.13644582786162077No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23230.13528111742319462No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG23230.13528111742319462No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA23040.1341746425066898No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA21580.1256722563061791No Hit
CTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTA21380.12450754586775295No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG19440.11320985461501952No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG19310.11245279283004254No Hit
CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG19010.11070572717240335No Hit
ACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGAT18800.10948278121205592No Hit
AGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACA18730.10907513255860676No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG18230.10616335646254145No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC18010.10488217498027272No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA17870.10406687767337441No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA17350.1010386305334665No Hit
CGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTG17270.10057274635809604No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACT1000.056.8914031
GTAAGCG1050.036.24746394-95
GCTTATG5200.031.9102441
GAACCCG804.5934157E-429.5774029
TATGTAC5900.028.876414
GTATAGC2253.6379788E-1227.3921591
GCGTGGC700.00854618327.0421949
CCGTATC1007.057679E-1026.16613894-95
GCGCCGT659.906953E-625.61719994-95
AGTACTC4650.025.4436665
ACCACTC10100.024.96499394-95
TTATGTA6900.024.691423
TAGGGTC1151.3146029E-424.6914184
GTCACTA3500.024.3386862
CTTATGT6950.023.8328462
ATAGCCC2202.2646418E-923.662613
TAGCCCT3800.023.662614
TACCTGG6850.023.4898912
TCTACAC2453.110472E-1023.17973
GTCCTAC1452.3698825E-522.8873481
Produced by FastQC (version 0.11.5)