##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938986_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1717165 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.804777059863206 33.0 31.0 34.0 30.0 34.0 2 31.790449956760124 33.0 31.0 34.0 30.0 34.0 3 31.842880561856315 33.0 31.0 34.0 30.0 34.0 4 35.27288583217105 37.0 35.0 37.0 33.0 37.0 5 35.35208031843183 37.0 35.0 37.0 33.0 37.0 6 35.495401432011484 37.0 35.0 37.0 33.0 37.0 7 35.45764617843946 37.0 35.0 37.0 33.0 37.0 8 35.49154216397376 37.0 35.0 37.0 33.0 37.0 9 36.97771035398462 39.0 37.0 39.0 33.0 39.0 10-11 36.87542810388052 39.0 37.0 39.0 32.5 39.0 12-13 36.778805472974355 39.0 37.0 39.0 32.5 39.0 14-15 37.601873436740206 40.0 37.0 41.0 32.0 41.0 16-17 37.572320656430804 39.5 37.0 41.0 32.0 41.0 18-19 37.75504829180655 40.0 37.0 41.0 32.5 41.0 20-21 37.56207819283529 40.0 37.0 41.0 32.0 41.0 22-23 37.81572708505007 40.0 38.0 41.0 32.5 41.0 24-25 37.75580855654523 40.0 37.5 41.0 32.0 41.0 26-27 37.35343545902694 39.5 36.5 41.0 31.0 41.0 28-29 37.14057123223452 39.0 36.5 41.0 30.5 41.0 30-31 37.18902173058501 39.0 36.5 41.0 31.0 41.0 32-33 37.12032099419683 39.5 36.0 41.0 31.0 41.0 34-35 36.87886254378583 39.5 36.0 41.0 30.5 41.0 36-37 36.39314509671465 39.0 35.0 41.0 30.0 41.0 38-39 35.873688900018344 38.5 35.0 40.0 28.5 41.0 40-41 35.62605864899413 38.0 35.0 40.0 29.0 41.0 42-43 35.75661395381341 38.0 35.0 40.0 29.0 41.0 44-45 35.907806762891155 39.0 35.0 40.5 29.5 41.0 46-47 35.61759761001419 39.0 35.0 41.0 27.0 41.0 48-49 35.46812944591812 39.0 35.0 41.0 26.5 41.0 50-51 34.22720093875661 38.0 33.5 39.5 24.5 40.5 52-53 34.076048021011374 37.5 33.5 39.5 23.5 40.5 54-55 34.65801306222757 38.0 34.0 40.0 24.0 41.0 56-57 34.87062745863094 38.0 34.0 40.0 25.0 41.0 58-59 34.81832118637405 38.5 34.0 40.0 25.0 41.0 60-61 34.42552695867899 38.0 34.0 40.0 23.0 41.0 62-63 33.788668532144555 37.5 33.5 40.0 22.0 41.0 64-65 32.948028581994166 36.0 32.0 39.0 20.0 41.0 66-67 32.522892674844876 36.0 31.5 39.0 19.5 40.0 68-69 32.26753806419302 36.0 32.0 38.0 19.5 39.5 70-71 31.576736073702875 35.0 31.0 37.0 15.5 39.0 72-73 30.877703656899598 35.0 30.5 37.0 11.5 39.0 74-75 30.387431900836553 35.0 30.0 36.0 7.5 37.0 76-77 29.678328232872204 34.0 29.5 35.5 4.5 37.0 78-79 29.21244755163307 34.0 29.0 35.0 2.0 36.0 80-81 28.995931375260966 34.0 29.0 35.0 2.0 36.0 82-83 28.648543092830334 34.0 29.0 35.0 2.0 36.0 84-85 28.344501547608992 34.0 29.0 35.0 2.0 35.0 86-87 27.699628748547752 33.5 27.0 35.0 2.0 35.0 88-89 26.915313321666815 33.0 25.5 35.0 2.0 35.0 90-91 26.218129882684543 32.0 24.5 35.0 2.0 35.0 92-93 25.977018224806585 32.0 24.0 35.0 2.0 35.0 94-95 25.536596366685785 32.0 21.5 35.0 2.0 35.0 96-97 24.645004120163176 32.0 14.5 34.0 2.0 35.0 98-99 23.724311874514097 31.0 2.0 34.0 2.0 35.0 100-101 20.99780888848771 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26864697426383444 1101 2 0.29889133567092685 1101 3 0.37188082637124253 1101 4 0.16515403116900274 1101 5 0.09156705749668248 1101 6 0.16175839449602591 1101 7 0.16612062617740975 1101 8 0.22445083687918554 1101 9 0.16322691140065615 1101 10-11 0.38391401867548325 1101 12-13 0.5053993845945897 1101 14-15 0.12630872380056246 1101 16-17 0.5354059364026469 1101 18-19 0.5178842661830814 1101 20-21 0.6617016282613548 1101 22-23 0.5602944229615545 1101 24-25 0.5380020061153274 1101 26-27 0.5909663039599025 1101 28-29 0.6057745888092754 1101 30-31 0.5596527531095958 1101 32-33 0.5369079125674432 1101 34-35 0.503251158577747 1101 36-37 0.5124246393145668 1101 38-39 0.4676588341098835 1101 40-41 0.4957491853939331 1101 42-43 0.4656442362734623 1101 44-45 0.5249768617788035 1101 46-47 0.6495597309019274 1101 48-49 0.8273366556168682 1101 50-51 0.8193693749501207 1101 52-53 0.7457705893642625 1101 54-55 0.6032263782386451 1101 56-57 0.75866211268076 1101 58-59 0.6359645124305473 1101 60-61 0.6935326243146562 1101 62-63 0.8403285439324222 1101 64-65 0.6968110914182404 1101 66-67 0.8081476622325852 1101 68-69 0.9909736643433078 1101 70-71 0.8905687599428695 1101 72-73 0.7588841927844001 1101 74-75 0.8713176159449247 1101 76-77 0.7745297702371694 1101 78-79 0.6623947671504027 1101 80-81 0.6462021118768426 1101 82-83 0.6518076529968297 1101 84-85 0.8358646963179446 1101 86-87 0.5480067562189923 1101 88-89 0.6938216526005405 1101 90-91 0.760835979425007 1101 92-93 0.8404391022291264 1101 94-95 0.9992293773442853 1101 96-97 0.8071618452790119 1101 98-99 0.821814837651246 1101 100-101 1.250976517722325 1102 1 0.28231977247795825 1102 2 0.2186145871003582 1102 3 0.15532217826757133 1102 4 0.2213681508209291 1102 5 0.3308203992607375 1102 6 0.28704707587274925 1102 7 0.37339983070840077 1102 8 0.15570746353850495 1102 9 0.4406791127045864 1102 10-11 0.3046001839458157 1102 12-13 0.30417506711639675 1102 14-15 0.5409182183816412 1102 16-17 0.4649175588401775 1102 18-19 0.5165319457438571 1102 20-21 0.6442128381592269 1102 22-23 0.6214750189464269 1102 24-25 0.5794393020142152 1102 26-27 0.5080581692175059 1102 28-29 0.6615530357323891 1102 30-31 0.5403827169834656 1102 32-33 0.5371207367677187 1102 34-35 0.5697938063112886 1102 36-37 0.6346100199884361 1102 38-39 0.6373834349681218 1102 40-41 0.4874762229350367 1102 42-43 0.35359884919945017 1102 44-45 0.5586441466123873 1102 46-47 0.5895859098207623 1102 48-49 0.7201756224941747 1102 50-51 0.6855590506630946 1102 52-53 0.7953225790006471 1102 54-55 0.6054704783431291 1102 56-57 0.6060661972089818 1102 58-59 0.6999906682163228 1102 60-61 0.5925901446813953 1102 62-63 0.5746183009966757 1102 64-65 0.6853216690123318 1102 66-67 0.7590228632010891 1102 68-69 0.7316672321945177 1102 70-71 0.708953570772227 1102 72-73 0.7058435441332485 1102 74-75 0.8623034315204166 1102 76-77 0.8225912193694889 1102 78-79 0.8398394502081281 1102 80-81 0.7442305963911835 1102 82-83 0.7370414318987031 1102 84-85 0.9110974727869916 1102 86-87 0.9275442105793807 1102 88-89 0.912569074227644 1102 90-91 0.9449611061749259 1102 92-93 1.0731916671077357 1102 94-95 1.1320584741137516 1102 96-97 0.9145308832133807 1102 98-99 1.2373928365639948 1102 100-101 1.316815727496813 1103 1 0.09949033942757879 1103 2 0.2637539373656459 1103 3 0.335116312546905 1103 4 0.24606384840659246 1103 5 0.23760528472436704 1103 6 0.2654005827282475 1103 7 0.23929895588724293 1103 8 0.040951693457941474 1103 9 0.31926252663930654 1103 10-11 0.3518879602437508 1103 12-13 0.4346454032318974 1103 14-15 0.34019682311760135 1103 16-17 0.37190214517739406 1103 18-19 0.5231216883035543 1103 20-21 0.5474363269991542 1103 22-23 0.5384284256810972 1103 24-25 0.63943879213965 1103 26-27 0.6073743965164979 1103 28-29 0.5126356694045029 1103 30-31 0.48728970968144125 1103 32-33 0.6356138256496706 1103 34-35 0.47830647128660786 1103 36-37 0.49468884968723614 1103 38-39 0.588746036131873 1103 40-41 0.6021829369015919 1103 42-43 0.5170410742302707 1103 44-45 0.7231922586859056 1103 46-47 0.5627487390375023 1103 48-49 0.5225184961472564 1103 50-51 0.6155026708961557 1103 52-53 0.7936573750044715 1103 54-55 0.8014488812923588 1103 56-57 0.8031060663102707 1103 58-59 0.7101634384421232 1103 60-61 0.6902006097797084 1103 62-63 0.65955297524863 1103 64-65 0.584331353539028 1103 66-67 0.49282623393552427 1103 68-69 0.636766441425408 1103 70-71 0.8245691747881843 1103 72-73 0.9221010493324293 1103 74-75 0.7748583975442287 1103 76-77 0.7848041059084885 1103 78-79 0.8973981089598944 1103 80-81 0.822160289323083 1103 82-83 0.8420238335006367 1103 84-85 0.7361591212578311 1103 86-87 0.838647192882096 1103 88-89 1.0360070039514788 1103 90-91 1.0885251757774377 1103 92-93 1.0909148299369562 1103 94-95 1.2383368069166067 1103 96-97 1.3196586763217333 1103 98-99 1.320247068059409 1103 100-101 1.5137428061627354 1104 1 0.12190152383719166 1104 2 0.21475254536661836 1104 3 0.20594788066407332 1104 4 0.3097341867228778 1104 5 0.21060352959515427 1104 6 0.29208762503291297 1104 7 0.28724121818827797 1104 8 0.21937268238247043 1104 9 0.2904148597507259 1104 10-11 0.20599738347197416 1104 12-13 0.31767284982149846 1104 14-15 0.35127002411015695 1104 16-17 0.1972357710322683 1104 18-19 0.3515086337735056 1104 20-21 0.32072431949445956 1104 22-23 0.4045423275149034 1104 24-25 0.40113158400553317 1104 26-27 0.44914798484791874 1104 28-29 0.4696970384052932 1104 30-31 0.4120370315963484 1104 32-33 0.5258615106947602 1104 34-35 0.6085907719583972 1104 36-37 0.38879706248152246 1104 38-39 0.5707453703932046 1104 40-41 0.5870540256869177 1104 42-43 0.5107067221150388 1104 44-45 0.510224728576361 1104 46-47 0.6216091154824568 1104 48-49 0.5099935309472343 1104 50-51 0.47268810937585926 1104 52-53 0.5144011866609546 1104 54-55 0.5043713227376259 1104 56-57 0.5614890778699149 1104 58-59 0.6393757485552953 1104 60-61 0.6882033937777692 1104 62-63 0.6568438727854016 1104 64-65 0.6945617899004617 1104 66-67 0.8590528574300507 1104 68-69 0.5995206325529239 1104 70-71 0.5497726789944402 1104 72-73 0.5722705100841061 1104 74-75 0.5683311325197167 1104 76-77 0.6168964922790039 1104 78-79 0.676086906393774 1104 80-81 0.567317040518386 1104 82-83 0.5000369061871979 1104 84-85 0.7462579229282298 1104 86-87 0.9614600900557342 1104 88-89 0.8886245673479571 1104 90-91 1.0971419523918549 1104 92-93 1.0830871049475448 1104 94-95 1.0289998221075543 1104 96-97 1.0951451194615984 1104 98-99 0.885571161746995 1104 100-101 1.1924437079360217 1105 1 0.15138631091458876 1105 2 0.1518911746728122 1105 3 0.2241093548669788 1105 4 0.16600195457090905 1105 5 0.19776548489671342 1105 6 0.09000505100932799 1105 7 0.15359201200106298 1105 8 0.19245958629394977 1105 9 0.3220820852693649 1105 10-11 0.26173995195564004 1105 12-13 0.31875149487274257 1105 14-15 0.41487582207788165 1105 16-17 0.39891469599545104 1105 18-19 0.4723217998400102 1105 20-21 0.4303504839718926 1105 22-23 0.42837238305993 1105 24-25 0.5199837263524145 1105 26-27 0.3963874990840637 1105 28-29 0.3884238035270968 1105 30-31 0.5014051158031876 1105 32-33 0.3262866634071315 1105 34-35 0.4826750444060295 1105 36-37 0.4608656409078904 1105 38-39 0.4661308324665967 1105 40-41 0.46613571040693813 1105 42-43 0.5116895119040876 1105 44-45 0.5049834867586824 1105 46-47 0.7691015709808298 1105 48-49 0.6821441148260519 1105 50-51 0.5853030992436246 1105 52-53 0.48930059330594844 1105 54-55 0.488460260384322 1105 56-57 0.5587553805538619 1105 58-59 0.5501301258241114 1105 60-61 0.5774796318778641 1105 62-63 0.4922099086782765 1105 64-65 0.46895804753608417 1105 66-67 0.45594448631346296 1105 68-69 0.44185202934269086 1105 70-71 0.4317517074667343 1105 72-73 0.5361061055530314 1105 74-75 0.42273245626995504 1105 76-77 0.4925793243464156 1105 78-79 0.7086445288916998 1105 80-81 0.6462248736792056 1105 82-83 0.7699682402883425 1105 84-85 0.8364768592628415 1105 86-87 0.6621294367820774 1105 88-89 0.8820141989430148 1105 90-91 1.006476724161569 1105 92-93 1.0221528623863492 1105 94-95 1.129217774376734 1105 96-97 1.060119861782244 1105 98-99 1.4983600031739641 1105 100-101 1.4934357916763439 1106 1 0.24027042627621498 1106 2 0.2659251532465383 1106 3 0.25889112644268764 1106 4 0.15535742781106165 1106 5 0.2970423194638201 1106 6 0.24839918694660668 1106 7 0.23518915439841237 1106 8 0.2761796711254334 1106 9 0.2283428616268992 1106 10-11 0.2554433262487166 1106 12-13 0.4104906265569568 1106 14-15 0.4740464664178532 1106 16-17 0.5891594342592725 1106 18-19 0.3761187416534355 1106 20-21 0.5212945000760811 1106 22-23 0.4491468655074087 1106 24-25 0.5142061618666958 1106 26-27 0.4992110125214637 1106 28-29 0.5399406476923403 1106 30-31 0.3535854603017796 1106 32-33 0.47018462279606865 1106 34-35 0.46175095019479784 1106 36-37 0.3303161759899709 1106 38-39 0.3426716719879508 1106 40-41 0.34501017015221436 1106 42-43 0.4572841728655064 1106 44-45 0.4889917313906196 1106 46-47 0.5532108978464265 1106 48-49 0.514832004211776 1106 50-51 0.506874026355213 1106 52-53 0.3536469066421901 1106 54-55 0.3893443527563818 1106 56-57 0.5347961252432754 1106 58-59 0.669753621444606 1106 60-61 0.643790870325617 1106 62-63 0.4754648859186048 1106 64-65 0.47616796954398666 1106 66-67 0.597732794109227 1106 68-69 0.4332052596636231 1106 70-71 0.5697905612646075 1106 72-73 0.4900193961066215 1106 74-75 0.465392284020659 1106 76-77 0.3980175054122128 1106 78-79 0.535803428438804 1106 80-81 0.5527356615315249 1106 82-83 0.6503461815155269 1106 84-85 0.7804848811833871 1106 86-87 1.043478148217087 1106 88-89 0.9640467061847282 1106 90-91 1.0977722291273153 1106 92-93 1.0668860613265316 1106 94-95 1.237386899968719 1106 96-97 1.2297179970413374 1106 98-99 1.2318378714092866 1106 100-101 1.7079309265101337 1107 1 0.03039468443645177 1107 2 -0.05503467327708478 1107 3 0.005611338722985693 1107 4 0.05400392689802658 1107 5 -0.04551574376375811 1107 6 -0.0247865155185778 1107 7 -0.06581660912589626 1107 8 0.04918740337834748 1107 9 0.07459015105853695 1107 10-11 0.04807236356895572 1107 12-13 0.0662063592663813 1107 14-15 0.04626260571253482 1107 16-17 -0.06790409290346844 1107 18-19 -0.028404697629838438 1107 20-21 0.04314506929543427 1107 22-23 -0.028853127359518282 1107 24-25 0.21384237506003245 1107 26-27 0.24863412510990912 1107 28-29 0.20721784610594085 1107 30-31 0.16389524973342162 1107 32-33 0.2284063829535654 1107 34-35 0.14533988536941678 1107 36-37 0.26856835871080165 1107 38-39 0.43512077750039424 1107 40-41 0.30861080356923054 1107 42-43 0.1648293608933571 1107 44-45 0.28044167147279353 1107 46-47 0.34703793505254765 1107 48-49 0.09443479895041662 1107 50-51 0.19440773013288748 1107 52-53 0.15832352870335598 1107 54-55 0.15032286793557148 1107 56-57 0.250639219000405 1107 58-59 0.393213412302849 1107 60-61 0.42251076875062665 1107 62-63 0.20931275170267583 1107 64-65 0.2974468228309135 1107 66-67 0.046104134841932876 1107 68-69 0.11367258304868955 1107 70-71 0.18045860937006353 1107 72-73 0.16919277975190639 1107 74-75 0.2446031163078075 1107 76-77 0.23687278959390667 1107 78-79 0.26214212822196004 1107 80-81 0.11086665331422196 1107 82-83 0.3213439916616103 1107 84-85 0.3717256438517289 1107 86-87 0.34437334908446715 1107 88-89 0.4270174891109981 1107 90-91 0.4356235330533593 1107 92-93 0.46503235355433503 1107 94-95 0.3797669929840133 1107 96-97 0.3900219016863957 1107 98-99 0.4152682740000202 1107 100-101 0.20576425508808072 1108 1 0.1026859668167539 1108 2 0.1439512562726435 1108 3 0.053406314707874714 1108 4 0.05297559886673042 1108 5 -0.0631238563273584 1108 6 0.10739195375187904 1108 7 0.11163146209072039 1108 8 0.07390648447724857 1108 9 0.28585542226446137 1108 10-11 0.16435452109740822 1108 12-13 0.19984801278934583 1108 14-15 0.2971033574434969 1108 16-17 0.10345510011744352 1108 18-19 0.1546938311660213 1108 20-21 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-3.255877826021912 2316 66-67 -3.2066312584385486 2316 68-69 -3.2824461520218264 2316 70-71 -3.266673258554107 2316 72-73 -3.299906268396075 2316 74-75 -3.6245662993943384 2316 76-77 -3.48800650242708 2316 78-79 -3.2005179979453224 2316 80-81 -3.2131955609084564 2316 82-83 -3.52126661552024 2316 84-85 -3.846151494980255 2316 86-87 -3.8063580227775233 2316 88-89 -4.020335949376271 2316 90-91 -4.406419190519916 2316 92-93 -4.771709832230549 2316 94-95 -5.311229740158669 2316 96-97 -5.709533212537302 2316 98-99 -6.1454224329758205 2316 100-101 -5.862083006610888 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5574.0 3 1406.0 4 1222.0 5 1299.0 6 1533.0 7 1931.0 8 2435.0 9 3139.0 10 4092.0 11 5657.0 12 7816.0 13 11280.0 14 10840.0 15 10593.0 16 12641.0 17 15742.0 18 14373.0 19 11668.0 20 12049.0 21 13231.0 22 14596.0 23 16565.0 24 18725.0 25 21707.0 26 24949.0 27 29440.0 28 34359.0 29 40654.0 30 48951.0 31 58516.0 32 72493.0 33 92624.0 34 123175.0 35 179500.0 36 258489.0 37 336373.0 38 181460.0 39 16045.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.93025684029558 14.227008933524719 12.078945309169564 18.763788917010135 2 11.727074293074859 23.460443374589453 39.95558677045296 24.856895561882723 3 29.213316142389512 28.940530370149308 29.244733136243674 12.60142035121751 4 11.561452497682742 12.509983152519252 41.429412553413506 34.499151796384496 5 12.622328192542112 42.44099652260598 32.49786488589892 12.438810398952993 6 23.976062519997924 35.568477266912346 19.60796198631195 20.847498226777784 7 24.73573739685234 39.912847985833714 17.592734534335342 17.758680082978604 8 17.6898681978018 28.775870982708284 22.06516727798997 31.469093541499944 9 24.397667963408338 26.69694599267187 22.547625237383 26.35776080653679 10-11 21.623127570169583 21.806694979392017 27.818316349680906 28.7518611007575 12-13 37.86077311741372 19.444947172728988 22.893721492027606 19.80055821782969 14-15 31.704942706897267 21.669745499356736 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40-41 21.329423007409424 23.45978964436815 36.617977840510804 18.592809507711625 42-43 17.85064242884859 31.213607482370424 30.42659664508891 20.50915344369207 44-45 19.423382026610973 33.19469912286507 23.882029246420625 23.49988960410333 46-47 19.232534988581133 27.668940680447385 20.177841541254317 32.92068278971716 48-49 23.694460502740846 25.445478618256324 24.31929906561215 26.540761813390684 50-51 30.198097938843205 27.653065497446942 24.899478085475913 17.24935847823394 52-53 21.063210834168416 27.353223853865465 32.26266872748421 19.320896584481904 54-55 15.76403275419566 25.979382952650482 34.63447272216179 23.622111570992068 56-57 22.49156482889322 33.20260745292668 22.857689017796556 21.44813870038354 58-59 19.430284927788904 27.781732750157044 25.948690868365574 26.839291453688475 60-61 20.46369448816041 22.1020411089041 29.59509897460339 27.8391654283321 62-63 32.70348309514245 20.537361204965176 25.47050653087659 21.288649169015784 64-65 28.23843863510025 22.9281390931089 30.942294659065016 17.891127612725835 66-67 19.58698836725984 24.957211680837656 33.70790992937987 21.747890022522633 68-69 18.123360333516512 33.09873677574928 29.604482272561633 19.173420618172575 70-71 19.77328034362079 30.73831955280557 23.18954093677907 26.298859166794568 72-73 19.125236362443502 27.428265316403493 21.29221427383131 32.154284047321696 74-75 21.889423528919032 27.836863490282028 25.88243184706399 24.391281133734953 76-77 27.235205028252295 21.443806984829035 28.424448541469495 22.89653944544918 78-79 21.3942236612014 22.112672303980478 33.51565846227984 22.977445572538283 80-81 19.393398066892907 32.34113461723121 28.60768394846609 19.65778336740979 82-83 19.111595322718873 30.01139250624304 24.884179114441256 25.992833056596837 84-85 21.254450490516604 23.81041342308056 25.404464267674516 29.530671818728326 86-87 25.108120286618675 24.24424131926806 28.297167337791755 22.350471056321513 88-89 29.00676695230044 18.501285073788154 30.51605881031772 21.975889163593685 90-91 21.156999572703015 22.387814040586477 34.500263143482464 21.954923243228045 92-93 18.37907103096905 28.977759162794374 30.430713941564896 22.212455864671675 94-95 18.85266596138278 31.07197293209475 25.35905627860822 24.716304827914247 96-97 17.975725387182155 26.142025903034515 22.551648938055248 33.33059977172808 98-99 21.97692571298024 24.70275526552418 24.77797076319713 28.542348258298457 100-101 27.29647832717613 25.243779736735455 25.75476549121397 21.704976444874447 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8308.0 1 4980.0 2 1743.0 3 1680.0 4 1254.0 5 1195.5 6 2124.5 7 3729.0 8 4965.0 9 4839.0 10 3493.0 11 2390.0 12 2174.5 13 2131.0 14 2100.0 15 2593.0 16 3441.5 17 3209.0 18 2334.0 19 2079.0 20 2144.0 21 2245.5 22 2510.5 23 2932.0 24 3346.0 25 3874.5 26 4662.5 27 5511.5 28 6156.0 29 6989.0 30 7920.0 31 8929.0 32 10401.0 33 11720.5 34 13289.5 35 14559.0 36 15540.5 37 16966.0 38 17071.0 39 16155.5 40 15846.5 41 16069.5 42 16602.5 43 17479.5 44 21402.0 45 27692.5 46 51530.5 47 117271.0 48 423329.0 49 427991.0 50 131673.0 51 65764.0 52 30108.5 53 18009.5 54 13392.0 55 10412.5 56 8874.5 57 8201.5 58 9355.0 59 9899.0 60 10397.5 61 11056.5 62 9276.0 63 5544.0 64 2520.5 65 1758.5 66 1194.5 67 809.5 68 701.5 69 624.0 70 526.5 71 473.5 72 376.5 73 350.0 74 299.0 75 272.0 76 239.5 77 125.0 78 60.0 79 34.5 80 22.0 81 11.0 82 14.5 83 16.0 84 8.0 85 3.5 86 4.5 87 4.5 88 2.5 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27597813838507074 2 0.08502386200510725 3 0.08927505510536263 4 0.10336805141031874 5 0.10482393945835142 6 0.07931678085681923 7 0.08979917480265437 8 0.08665445661890384 9 0.09218683120142794 10-11 0.08423768245916961 12-13 0.08889652421287413 14-15 0.09830156100316509 16-17 0.08642151453121862 18-19 0.09795214787163727 20-21 0.09967009576831579 22-23 0.11219073298139666 24-25 0.12494431228216275 26-27 0.1273902042028576 28-29 0.11900428904618951 30-31 0.11230720402523928 32-33 0.10729894914000694 34-35 0.3720667495552262 36-37 0.5688154603663597 38-39 0.5532374582524102 40-41 0.5164034906371839 42-43 0.5422018268483226 44-45 0.5633704390667176 46-47 0.5511992149851646 48-49 0.5732704777933396 50-51 0.5649719159195534 52-53 0.5679128097765794 54-55 0.5589154216397375 56-57 0.5321561993168973 58-59 0.5269150023439797 60-61 0.4784921658664136 62-63 0.5492192072398401 64-65 0.5818893350376929 66-67 0.594904974187105 68-69 0.597671161478367 70-71 0.5920805513739216 72-73 0.5847428756118369 74-75 0.5819766883205748 76-77 0.5644769139832223 78-79 0.5426677110236932 80-81 0.5381544580747919 82-83 0.5511118617022825 84-85 0.5607207228192981 86-87 0.5509371551365186 88-89 0.5634577923495995 90-91 0.5777837307422409 92-93 0.5857037617235386 94-95 0.58532523083105 96-97 0.581801981754811 98-99 0.5864317057475549 100-101 0.5637780877201667 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1717165.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.52970025626514 #Duplication Level Percentage of deduplicated Percentage of total 1 77.60654418041295 15.156325457680628 2 11.815730133315858 4.61515335625157 3 4.163185826092166 2.439173138841348 4 1.9802323070180015 1.546933735753359 5 1.1226028637683403 1.0962048718110269 6 0.669541100428193 0.7845562200367516 7 0.4443570574275356 0.6074712096821037 8 0.3102772849746028 0.48476978975054014 9 0.23853058692737067 0.4192587778178291 >10 1.3285181391458718 4.932855712571644 >50 0.13388300464032768 1.8384170562918178 >100 0.13538054119372506 5.509868567150777 >500 0.020966013016724336 2.8229338823788668 >1k 0.019468440658386883 7.964990426064716 >5k 0.004492717075012358 5.722319786810556 >10k+ 0.0062898039050173 44.05876801110647 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 135266 7.877286108207423 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 109629 6.384302032710893 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 77176 4.494384639798739 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 49883 2.904962540000524 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 47164 2.746620155896492 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 45292 2.637603258859807 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 36324 2.1153470982695315 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 33942 1.9766300850529797 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 24933 1.451986268063931 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 24311 1.4157637734288786 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 21243 1.2370971921743106 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 21083 1.2277795086669014 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 20536 1.195924678175947 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 18350 1.0686218272559713 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 18183 1.0588964950951132 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 12377 0.7207810548200085 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 12188 0.7097745411768817 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 12148 0.7074451203000295 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11687 0.6805985446943071 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11308 0.6585272818861321 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 10192 0.5935364394219542 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 9231 0.5375721028555788 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 7834 0.45621707873151385 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 7817 0.45522707485885167 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 7559 0.4402023102031546 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7463 0.43461170009870925 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6912 0.40252392752006944 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6654 0.38749916286437236 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6130 0.3569837493776079 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5952 0.34661782647561534 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 5859 0.34120192293693385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5553 0.3233818532290141 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5476 0.3188977180410735 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 5209 0.30334883368808474 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5127 0.29857352089053757 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 5051 0.2941476212245183 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4806 0.2798799183537983 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4491 0.26153572894858673 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 4282 0.24936450486703374 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4213 0.2453462538544636 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4045 0.23556268617168416 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 3932 0.22898207219457656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3929 0.2288073656288126 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3529 0.2055131568602901 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3203 0.1865283767139442 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3185 0.1854801373193607 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 2963 0.17255185145283067 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 2781 0.16195298646315293 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2721 0.15845885514787456 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2669 0.15543060800796663 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2575 0.14995646894736384 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2569 0.14960705581583597 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2521 0.1468117507636133 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 2513 0.14634586658824283 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2506 0.1459382179347937 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2490 0.1450064495840528 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2344 0.13650406338354207 No Hit CAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTA 2343 0.13644582786162077 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2323 0.13528111742319462 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2323 0.13528111742319462 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 2304 0.1341746425066898 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2158 0.1256722563061791 No Hit CTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTA 2138 0.12450754586775295 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1944 0.11320985461501952 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1931 0.11245279283004254 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1901 0.11070572717240335 No Hit ACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGAT 1880 0.10948278121205592 No Hit AGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACA 1873 0.10907513255860676 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1823 0.10616335646254145 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1801 0.10488217498027272 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1787 0.10406687767337441 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1735 0.1010386305334665 No Hit CGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTG 1727 0.10057274635809604 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1647104384261268E-4 0.0 10-11 0.0 0.0 0.0 2.0382432672457217E-4 0.0 12-13 0.0 0.0 0.0 5.532374582524102E-4 0.0 14-15 0.0 0.0 0.0 8.444150678589419E-4 0.0 16-17 0.0 0.0 0.0 0.0012520637213080863 0.0 18-19 0.0 0.0 0.0 0.0016597123747572307 0.0 20-21 0.0 0.0 0.0 0.002766187291262051 0.0 22-23 0.0 0.0 0.0 0.005095608168114305 0.0 24-25 0.0 0.0 0.0 0.007861795459376356 0.0 26-27 0.0 0.0 0.0 0.010773571555441673 0.0 28-29 0.0 0.0 0.0 0.02105214117455224 0.0 30-31 0.0 0.0 0.0 0.047520185887785976 0.0 32-33 0.0 0.0 0.0 0.09049800106571006 0.0 34-35 0.0 0.0 0.0 0.19736018379130718 0.0 36-37 0.0 0.0 0.0 0.35896375712293227 0.0 38-39 0.0 0.0 0.0 0.5096190523333518 0.0 40-41 0.0 0.0 0.0 0.6679905541983444 0.0 42-43 0.0 0.0 0.0 0.8311956043828054 0.0 44-45 0.0 0.0 0.0 1.006368054322095 0.0 46-47 0.0 0.0 0.0 1.2593722793092104 0.0 48-49 0.0 0.0 0.0 1.5918388739579483 0.0 50-51 0.0 0.0 0.0 1.8758535143681592 0.0 52-53 0.0 0.0 0.0 2.103204991948939 0.0 54-55 0.0 0.0 0.0 2.3346329560642105 0.0 56-57 0.0 0.0 0.0 2.5731365360929206 0.0 58-59 0.0 0.0 0.0 3.0198612247512617 0.0 60-61 0.0 0.0 0.0 3.779776550302388 0.0 62-63 0.0 0.0 0.0 4.343641991305436 0.0 64-65 0.0 0.0 0.0 4.700276327551516 0.0 66-67 0.0 0.0 0.0 5.1157867764600375 0.0 68-69 0.0 0.0 0.0 5.563443233469119 0.0 70-71 0.0 0.0 0.0 6.113827151147385 0.0 72-73 0.0 0.0 0.0 6.831696429871329 0.0 74-75 0.0 0.0 0.0 7.518992059586585 0.0 76-77 0.0 0.0 0.0 8.040403805109003 0.0 78-79 0.0 0.0 0.0 8.618973715397182 0.0 80-81 0.0 0.0 0.0 9.255575323279942 0.0 82-83 0.0 0.0 0.0 9.900766670646094 0.0 84-85 0.0 0.0 0.0 11.149161554073139 0.0 86-87 0.0 0.0 0.0 12.59715868888546 0.0 88-89 0.0 0.0 0.0 13.456831463487784 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 100 0.0 56.891403 1 GTAAGCG 105 0.0 36.247463 94-95 GCTTATG 520 0.0 31.910244 1 GAACCCG 80 4.5934157E-4 29.577402 9 TATGTAC 590 0.0 28.87641 4 GTATAGC 225 3.6379788E-12 27.392159 1 GCGTGGC 70 0.008546183 27.042194 9 CCGTATC 100 7.057679E-10 26.166138 94-95 GCGCCGT 65 9.906953E-6 25.617199 94-95 AGTACTC 465 0.0 25.443666 5 ACCACTC 1010 0.0 24.964993 94-95 TTATGTA 690 0.0 24.69142 3 TAGGGTC 115 1.3146029E-4 24.691418 4 GTCACTA 350 0.0 24.338686 2 CTTATGT 695 0.0 23.832846 2 ATAGCCC 220 2.2646418E-9 23.66261 3 TAGCCCT 380 0.0 23.66261 4 TACCTGG 685 0.0 23.489891 2 TCTACAC 245 3.110472E-10 23.1797 3 GTCCTAC 145 2.3698825E-5 22.887348 1 >>END_MODULE