##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938986_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1717165 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.999213820454063 33.0 31.0 34.0 30.0 34.0 2 32.19990565845449 33.0 31.0 34.0 30.0 34.0 3 32.32335681195459 34.0 31.0 34.0 30.0 34.0 4 35.773098100648454 37.0 35.0 37.0 35.0 37.0 5 35.70703572458092 37.0 35.0 37.0 33.0 37.0 6 35.82283647756622 37.0 35.0 37.0 35.0 37.0 7 35.806145594628354 37.0 35.0 37.0 33.0 37.0 8 35.83883785192454 37.0 35.0 37.0 35.0 37.0 9 37.4052831265487 39.0 37.0 39.0 34.0 39.0 10-11 37.31594401236923 39.0 37.0 39.0 33.5 39.0 12-13 37.245077205743186 39.0 37.0 39.0 33.5 39.0 14-15 38.020387965047036 40.0 37.0 41.0 33.0 41.0 16-17 38.06247827087088 39.5 37.5 41.0 33.5 41.0 18-19 38.528717682925055 40.0 38.0 41.0 34.0 41.0 20-21 38.5352904351067 40.0 38.0 41.0 34.0 41.0 22-23 38.59652595993978 40.0 38.0 41.0 34.0 41.0 24-25 38.485244574633185 40.0 38.0 41.0 34.0 41.0 26-27 37.9207988166542 39.5 37.0 41.0 33.0 41.0 28-29 37.62811378056273 39.0 36.5 41.0 32.5 41.0 30-31 37.71144007710383 39.5 37.0 41.0 33.0 41.0 32-33 37.715747758660356 40.0 36.5 41.0 33.0 41.0 34-35 37.856193784522745 40.0 37.0 41.0 33.0 41.0 36-37 37.560677337355465 40.0 37.0 41.0 33.0 41.0 38-39 37.17155777109363 39.5 35.5 41.0 31.5 41.0 40-41 36.86622601788413 39.0 35.0 40.5 30.5 41.0 42-43 37.04556987825864 39.0 36.0 41.0 31.0 41.0 44-45 37.043091374445666 40.0 36.0 41.0 31.0 41.0 46-47 36.72292790733564 39.5 36.0 41.0 30.5 41.0 48-49 36.5003729985179 39.0 36.0 41.0 30.0 41.0 50-51 36.313712135991594 39.0 35.0 41.0 30.0 41.0 52-53 35.561086150719355 38.0 34.5 40.0 28.0 41.0 54-55 35.45273197392213 38.0 34.5 40.0 28.0 41.0 56-57 35.56904636421078 38.0 35.0 40.0 28.0 41.0 58-59 35.481267088485964 38.0 35.0 40.0 28.0 41.0 60-61 34.93094577399376 38.0 34.0 40.0 27.0 41.0 62-63 34.33250270067233 37.0 33.5 39.0 26.0 41.0 64-65 33.586692309708155 36.0 32.0 39.0 24.5 40.5 66-67 33.13242117094164 36.0 32.0 39.0 22.5 40.0 68-69 32.9108026893164 36.0 32.0 38.0 25.0 39.0 70-71 32.30747802336992 35.0 31.5 37.0 23.0 39.0 72-73 31.568769745481653 35.0 31.0 36.5 21.5 38.5 74-75 31.090692216531316 34.0 31.0 36.0 20.5 37.0 76-77 28.827432133778643 31.5 28.0 34.0 15.5 36.0 78-79 29.692452676358997 33.5 29.0 35.0 15.5 36.0 80-81 29.998187419380198 34.0 29.5 35.0 18.0 36.0 82-83 29.836332559771485 34.0 30.0 35.0 16.5 35.5 84-85 29.599230417577807 34.0 30.0 35.0 9.0 35.0 86-87 28.873032003331073 33.5 29.0 35.0 4.0 35.0 88-89 28.005784534392443 33.0 27.5 35.0 2.0 35.0 90-91 27.257977829736802 32.0 25.0 34.0 2.0 35.0 92-93 27.221054470595426 32.0 25.5 34.0 2.0 35.0 94-95 27.020190546627724 32.5 25.5 34.0 2.0 35.0 96-97 26.084411806669713 32.0 25.0 34.0 2.0 35.0 98-99 25.059829719333905 32.0 21.0 34.0 2.0 35.0 100-101 20.956298026106985 27.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1924415566984905 1101 2 0.14474115182368053 1101 3 0.16984426270330744 1101 4 0.10544789217158268 1101 5 0.07168532550462459 1101 6 0.15970101134900716 1101 7 0.09644891675146283 1101 8 0.17974345864034547 1101 9 0.23692307226337306 1101 10-11 0.22680591229747193 1101 12-13 0.19573534924373348 1101 14-15 0.29807924141127273 1101 16-17 0.20207605515237503 1101 18-19 0.33181397583813066 1101 20-21 0.25597479237068654 1101 22-23 0.27352142485420927 1101 24-25 0.28472469988007276 1101 26-27 0.27068874633326345 1101 28-29 0.3302371386692826 1101 30-31 0.21696867265242048 1101 32-33 0.2655302524299046 1101 34-35 0.3953483275482128 1101 36-37 0.33757444040622175 1101 38-39 0.359251438425801 1101 40-41 0.41135920661262304 1101 42-43 0.4223950018862368 1101 44-45 0.49395829219530896 1101 46-47 0.5013180330119553 1101 48-49 0.36779803252704824 1101 50-51 0.18770074732399422 1101 52-53 0.5095667449028483 1101 54-55 0.3979331617026034 1101 56-57 0.37128490212479903 1101 58-59 0.6300634821426172 1101 60-61 0.585797698999464 1101 62-63 0.4558022348731896 1101 64-65 0.334646405323376 1101 66-67 0.34384605972203275 1101 68-69 0.2593603225684902 1101 70-71 0.42698362271954693 1101 72-73 0.5140526133037255 1101 74-75 0.5139100768247538 1101 76-77 0.4200306530867728 1101 78-79 0.5337902492256958 1101 80-81 0.6280923629245976 1101 82-83 0.617587706827436 1101 84-85 0.5136331506869602 1101 86-87 0.7303310946367105 1101 88-89 0.7863175191918295 1101 90-91 0.7753492553377939 1101 92-93 0.60340315458091 1101 94-95 0.6052843377407804 1101 96-97 0.3563550808000109 1101 98-99 0.3057550916575913 1101 100-101 0.7421765439321497 1102 1 0.17053068839888397 1102 2 0.06827711795687463 1102 3 0.12347480451825277 1102 4 0.060607144048248074 1102 5 0.05490074446371551 1102 6 0.16524947704845516 1102 7 0.08908951759427453 1102 8 0.18113452696385224 1102 9 0.07686809267830341 1102 10-11 0.13398988570986603 1102 12-13 0.13690642211675197 1102 14-15 0.24293232716939883 1102 16-17 0.32975496049572683 1102 18-19 0.13404303945020501 1102 20-21 0.21937249988392438 1102 22-23 0.27314397033960347 1102 24-25 0.2498897735781611 1102 26-27 0.2544425873859737 1102 28-29 0.23512457702929623 1102 30-31 0.2518820586006214 1102 32-33 0.12612269023087208 1102 34-35 0.23691613249020094 1102 36-37 0.21760809901615374 1102 38-39 0.31230885854925816 1102 40-41 0.2817552446896414 1102 42-43 0.2593868937468713 1102 44-45 0.19679980370979422 1102 46-47 0.13323934974602736 1102 48-49 -0.08195617865470695 1102 50-51 -0.0868831466648814 1102 52-53 0.10558053220973562 1102 54-55 -0.06621000883820471 1102 56-57 -0.08119226361204568 1102 58-59 -0.11912107730743315 1102 60-61 -0.26042216536365714 1102 62-63 -0.25631004531449264 1102 64-65 -0.16929257736152437 1102 66-67 -0.0674120320314131 1102 68-69 -0.10938362909882926 1102 70-71 -0.07524640477024036 1102 72-73 9.178516321277641E-4 1102 74-75 0.14039107493940506 1102 76-77 0.16377759277501625 1102 78-79 -0.04293673048275437 1102 80-81 0.12366720031842604 1102 82-83 -0.008555741310157572 1102 84-85 0.21059674309777066 1102 86-87 0.40448413528536165 1102 88-89 0.46090796754918983 1102 90-91 0.5861363964379258 1102 92-93 0.44917539413151175 1102 94-95 0.4372197270895448 1102 96-97 0.682529180249059 1102 98-99 0.7614095839060901 1102 100-101 1.0830314649134536 1103 1 0.05695999042694666 1103 2 0.0920527237033113 1103 3 0.07648831773945375 1103 4 0.1685135990603186 1103 5 0.05329077687937911 1103 6 0.14107215194316325 1103 7 0.130398628280318 1103 8 0.16986629894794447 1103 9 0.08368727879488347 1103 10-11 0.09848446095345054 1103 12-13 0.12491664432935323 1103 14-15 0.11506312390248752 1103 16-17 0.24850953777880846 1103 18-19 0.17060220498638046 1103 20-21 0.19667303849687556 1103 22-23 0.1544961577015158 1103 24-25 0.18692798944978506 1103 26-27 0.17865166377136177 1103 28-29 0.444639377297527 1103 30-31 0.23735140586847336 1103 32-33 0.11853096688572151 1103 34-35 0.26188221810587464 1103 36-37 0.03284616408851804 1103 38-39 0.13512203741634465 1103 40-41 0.1529547477937001 1103 42-43 0.0939642695306091 1103 44-45 0.1405181489968541 1103 46-47 0.08927618741123666 1103 48-49 0.1055347440328589 1103 50-51 -0.04047992549365631 1103 52-53 0.13574502083644546 1103 54-55 0.2836002560184525 1103 56-57 0.09054083095578136 1103 58-59 0.00874619461640691 1103 60-61 0.21430698308400764 1103 62-63 0.35279306986463155 1103 64-65 0.15684946442347325 1103 66-67 0.2721999688709076 1103 68-69 0.15675428733736396 1103 70-71 -0.01637481899852844 1103 72-73 0.19972374083792843 1103 74-75 0.34944119406464935 1103 76-77 0.2763080693686817 1103 78-79 0.4843230181645346 1103 80-81 0.5517675467241183 1103 82-83 0.518624436002586 1103 84-85 0.43667358274067425 1103 86-87 0.31122228224666415 1103 88-89 0.6284168285512024 1103 90-91 0.724609831972785 1103 92-93 0.6748072523307584 1103 94-95 0.5420271490092041 1103 96-97 0.48783473417369905 1103 98-99 0.7398496149311846 1103 100-101 1.3044000764168437 1104 1 0.05400804465438824 1104 2 0.0679817317527025 1104 3 0.11765820214301215 1104 4 0.07667797598944759 1104 5 0.07926668924395841 1104 6 0.017169107701796804 1104 7 0.05759253979759649 1104 8 0.07873726668987757 1104 9 0.020134957787803387 1104 10-11 0.10197958895683712 1104 12-13 0.07886417201802942 1104 14-15 0.19676817141201752 1104 16-17 0.040131065346443506 1104 18-19 0.07639413139200713 1104 20-21 0.13826354869478763 1104 22-23 0.09795603181638057 1104 24-25 0.04686035645335096 1104 26-27 0.10007785756183551 1104 28-29 0.0032228669785681063 1104 30-31 -0.06645086974733516 1104 32-33 0.019914644184034103 1104 34-35 0.12180853293974536 1104 36-37 0.07223898016307118 1104 38-39 0.18596177596261043 1104 40-41 0.07755833067816553 1104 42-43 0.06868738276670427 1104 44-45 0.1490675952233218 1104 46-47 0.047616077261750434 1104 48-49 0.15086927771318415 1104 50-51 0.07645707141973901 1104 52-53 0.13619590747930488 1104 54-55 0.20588358834309162 1104 56-57 0.17585524117183837 1104 58-59 0.16473557305857156 1104 60-61 0.3069513273314186 1104 62-63 0.1707960051669346 1104 64-65 0.18367419358887815 1104 66-67 0.1657642429131556 1104 68-69 0.3203406517990288 1104 70-71 0.33752126524768045 1104 72-73 0.20825049142801433 1104 74-75 0.15955966528658294 1104 76-77 0.17318514980201627 1104 78-79 0.3345076093404735 1104 80-81 0.4687204305829802 1104 82-83 0.6076730349252983 1104 84-85 0.5162694491087656 1104 86-87 0.4676451473437986 1104 88-89 0.6835528510652722 1104 90-91 0.7075238003804571 1104 92-93 0.5538939799226021 1104 94-95 0.7077771641396282 1104 96-97 0.6112067005236348 1104 98-99 0.6273995453312331 1104 100-101 1.0905285034579357 1105 1 -0.0010263611887708635 1105 2 0.017655532286283915 1105 3 0.04898501391680554 1105 4 0.05615693156800461 1105 5 3.1985793464173184E-4 1105 6 0.05006240585175448 1105 7 0.054774832451371935 1105 8 0.014466495619949171 1105 9 0.06934661203823822 1105 10-11 -0.010085417676492625 1105 12-13 -0.0029564948138585123 1105 14-15 -0.004615533922340376 1105 16-17 0.12201132280711136 1105 18-19 0.13290099069251937 1105 20-21 0.10982551565934529 1105 22-23 0.17736409823714894 1105 24-25 0.19986740527757263 1105 26-27 0.14827373760790863 1105 28-29 0.14859703052295714 1105 30-31 0.15586008034960486 1105 32-33 0.19516667427456724 1105 34-35 0.27222566734121045 1105 36-37 0.12286531958546476 1105 38-39 0.29922005304628385 1105 40-41 0.23389024501344835 1105 42-43 0.3277466162161815 1105 44-45 0.30542265331963137 1105 46-47 0.33470404542175913 1105 48-49 0.0941515732827014 1105 50-51 0.24308631930217217 1105 52-53 0.20937465749752704 1105 54-55 0.23797487316807775 1105 56-57 0.27872616094499136 1105 58-59 0.23739185722750733 1105 60-61 0.30514338045637146 1105 62-63 0.30944736171935006 1105 64-65 0.11115622528307512 1105 66-67 0.3410811484282945 1105 68-69 0.10390183880060988 1105 70-71 0.17887461034119667 1105 72-73 0.199745432602775 1105 74-75 0.14375320544374048 1105 76-77 0.08300867406834556 1105 78-79 0.17803974818846058 1105 80-81 0.2578124297665738 1105 82-83 0.4409927614280029 1105 84-85 0.28139799721134295 1105 86-87 0.15999786555119755 1105 88-89 0.409366527387931 1105 90-91 0.43732039041151083 1105 92-93 0.49800416609319953 1105 94-95 0.6030516455007024 1105 96-97 0.5126966484775117 1105 98-99 0.7887992143936735 1105 100-101 1.4199706477000582 1106 1 0.018847397374841535 1106 2 -0.08627776637112561 1106 3 -0.07995554082134504 1106 4 -0.024046109376406832 1106 5 -0.027683943964298408 1106 6 0.04160332439973757 1106 7 -0.01745729603729984 1106 8 0.0016939974591139162 1106 9 0.08283430112739154 1106 10-11 -0.021320129617265593 1106 12-13 0.03504381554523661 1106 14-15 0.12111151099927042 1106 16-17 -0.06799364150158738 1106 18-19 -0.02840330059674301 1106 20-21 0.07310759184675675 1106 22-23 0.22756144926478328 1106 24-25 0.03477809801057674 1106 26-27 0.019551942927691357 1106 28-29 0.0323797619129067 1106 30-31 0.1717981335359653 1106 32-33 0.18253337073188192 1106 34-35 0.11384212815550399 1106 36-37 0.07743363307114492 1106 38-39 0.31308419622527595 1106 40-41 0.3602477068507781 1106 42-43 0.4024804761062839 1106 44-45 0.42174876314077636 1106 46-47 0.45421492810602615 1106 48-49 0.25204335135295963 1106 50-51 0.4444184926204926 1106 52-53 0.5368965406875574 1106 54-55 0.48595951470331755 1106 56-57 0.5533587589954791 1106 58-59 0.5909228385369971 1106 60-61 0.6518651406522409 1106 62-63 0.4942613546040775 1106 64-65 0.40872989246317815 1106 66-67 0.4889144539825736 1106 68-69 0.5828069393224808 1106 70-71 0.47495970333397963 1106 72-73 0.6457050528726711 1106 74-75 0.5907466376130337 1106 76-77 0.38013871452997705 1106 78-79 0.68469522660126 1106 80-81 0.7296036974685371 1106 82-83 0.8285073454318628 1106 84-85 0.719939711772934 1106 86-87 0.7823276637603094 1106 88-89 0.772347117012739 1106 90-91 0.9073215255211728 1106 92-93 0.9534498546880776 1106 94-95 1.1875276918131767 1106 96-97 0.892650180823825 1106 98-99 0.7660399064944592 1106 100-101 1.011324704208402 1107 1 0.0018856591872093986 1107 2 -0.011863748452846323 1107 3 -0.036231933197512944 1107 4 -0.1266657310381305 1107 5 -0.0747754327796315 1107 6 -0.08439780262605723 1107 7 -0.05347896631805327 1107 8 -0.06822119028341689 1107 9 -0.05998554593949024 1107 10-11 0.004905332605964929 1107 12-13 0.014268022347337705 1107 14-15 0.01124218892667983 1107 16-17 -0.053918836947836724 1107 18-19 -0.1502560097384844 1107 20-21 -0.017989617694446736 1107 22-23 0.052217991720844736 1107 24-25 -0.08760352861576592 1107 26-27 -0.13663778259159898 1107 28-29 -0.06474774892937063 1107 30-31 -0.08579841262929477 1107 32-33 0.013383740225144436 1107 34-35 -0.17254172399012901 1107 36-37 0.10771068169017894 1107 38-39 -0.03436644289821089 1107 40-41 -0.03832246833754027 1107 42-43 -0.0815369785273603 1107 44-45 0.0026003809221037955 1107 46-47 -0.1312632018871227 1107 48-49 -0.08456804587370215 1107 50-51 0.10162896112083075 1107 52-53 0.07169203543761427 1107 54-55 0.05408055584689464 1107 56-57 0.00415552343282144 1107 58-59 0.06348439697114117 1107 60-61 -0.20124238331488442 1107 62-63 -0.21846993534113324 1107 64-65 -0.23239477941539377 1107 66-67 -0.233692042821815 1107 68-69 -0.2919621273860429 1107 70-71 -0.2301743271385135 1107 72-73 -0.25926687847861984 1107 74-75 -0.2467884927795687 1107 76-77 -0.3441322852417663 1107 78-79 -0.17286012605795165 1107 80-81 -0.05341263013931652 1107 82-83 -0.1573845028904337 1107 84-85 -0.2381734473231667 1107 86-87 -0.23391452552295888 1107 88-89 0.023412263383431053 1107 90-91 0.15377389084872206 1107 92-93 0.1077081219215863 1107 94-95 0.03326478808115141 1107 96-97 0.09746999935840606 1107 98-99 0.10966686095691003 1107 100-101 0.5506229523252735 1108 1 -0.07246049705805646 1108 2 -0.06653266068852304 1108 3 -0.014041790532594689 1108 4 -0.038315065832861706 1108 5 -0.012478714696563031 1108 6 -0.06402550154158604 1108 7 -0.023340222232725694 1108 8 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-1.3810320176171942 2316 54-55 -1.513502682136469 2316 56-57 -1.4445206506076573 2316 58-59 -1.5938402984056808 2316 60-61 -1.3354610781130631 2316 62-63 -1.3121007993645577 2316 64-65 -1.136235045067444 2316 66-67 -1.3403397412648559 2316 68-69 -1.4016235634947698 2316 70-71 -1.231751926994285 2316 72-73 -1.3866645729235074 2316 74-75 -1.624950707438412 2316 76-77 -1.410849159061403 2316 78-79 -1.7137614123012384 2316 80-81 -1.780503556941067 2316 82-83 -2.024946078135013 2316 84-85 -2.2260273386925284 2316 86-87 -2.362180619556689 2316 88-89 -2.6455297662552653 2316 90-91 -2.9757742302358103 2316 92-93 -3.386152745140439 2316 94-95 -3.6204268188433666 2316 96-97 -3.54747567659901 2316 98-99 -3.9766911383365056 2316 100-101 -4.466650246796004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 20.0 5 49.0 6 167.0 7 317.0 8 550.0 9 901.0 10 1561.0 11 2423.0 12 3672.0 13 5158.0 14 6239.0 15 8170.0 16 10340.0 17 13689.0 18 13633.0 19 10203.0 20 10162.0 21 11000.0 22 12130.0 23 13494.0 24 15844.0 25 18420.0 26 21968.0 27 26994.0 28 32896.0 29 40318.0 30 49173.0 31 59794.0 32 74080.0 33 94897.0 34 129922.0 35 191046.0 36 295367.0 37 360171.0 38 174448.0 39 7941.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.64079940320706 14.86708962885185 12.635839380383004 18.856271587558084 2 12.02971176328425 24.025705159376066 39.59339958594544 24.351183491394245 3 29.170114694860423 28.83328043606759 29.298524020696902 12.698080848375085 4 11.865021707290795 13.000847326843957 40.68455856018496 34.44957240568029 5 12.10083661686721 42.79901511454418 32.68658489570534 12.41356337288327 6 24.194588172947853 36.02443562499818 18.840239580937183 20.94073662111678 7 24.566713158025 39.37728756409547 17.719555197083565 18.336444080795964 8 18.085390745793212 29.202726587136357 22.01541494265257 30.696467724417865 9 24.209438231037787 26.62522238689934 22.11988946897939 27.045449913083488 10-11 21.80550500388722 22.084307565085474 27.657825543846982 28.452361887180324 12-13 37.572714328558995 19.590749869698023 23.18038744092734 19.65614836081565 14-15 31.39235677877637 21.943170121291622 30.30502710171113 16.35944599822088 16-17 18.89909715472414 26.32452346273747 34.47642486548752 20.299954517050864 18-19 17.55530190750452 34.871081113346705 30.432689927875305 17.140927051273465 20-21 20.36321495022319 30.837514158511265 23.264421299059787 25.534849592205756 22-23 18.81604272157888 28.149362466623767 20.560021896556243 32.47457291524111 24-25 23.184114514330307 27.942509892759286 25.874886804704268 22.998488788206142 26-27 29.14259841075261 20.82659499815102 29.362728683615146 20.668077907481226 28-29 21.557246973936692 22.608980499835486 34.24504925269267 21.588723273535155 30-31 19.218362824772225 34.14569945229491 28.63600760555916 17.999930117373694 32-33 18.95545856105849 31.493333488628057 25.04718533163674 24.504022618676714 34-35 22.354322759563725 24.152093628512517 25.73039901253635 27.763184599387408 36-37 26.845328210580327 24.493643295998854 29.340923114734004 19.32010537868682 38-39 31.538672779007825 17.866017536191073 32.27870015873954 18.316609526061562 40-41 21.404829521878558 23.650645424201418 36.2178325242782 18.726692529641817 42-43 17.79249984203481 31.209931802716184 30.570238310503363 20.42733004474564 44-45 19.57501872279662 33.11326039886836 23.718143583758746 23.59357729457627 46-47 19.43327288461516 27.888260719165036 20.137838218731183 32.540628177488614 48-49 23.8533544612299 25.336907837995387 24.47528216631987 26.334455534454836 50-51 30.02793283889132 27.90287653207676 24.72200375679649 17.347186872235433 52-53 21.15492687074335 27.521030302853834 32.142717793572544 19.181325032830276 54-55 15.586125969257466 26.192823636633637 34.32168719954111 23.89936319456779 56-57 22.574097422204623 33.2379241365856 22.49743035759522 21.690548083614562 58-59 19.604864549447402 27.965656043299454 25.65253168316486 26.77694772408829 60-61 20.52202788314575 22.496164385875154 29.232111362531853 27.749696368447246 62-63 32.51117394076865 20.940008677092766 25.379186042110106 21.169631340028477 64-65 28.07149595844301 23.281115329963427 30.53728248969228 18.11010622190128 66-67 19.448366510175514 25.387352723560415 33.27606745299514 21.888213313268924 68-69 18.341264160909514 33.22884751904185 29.142253480935437 19.2876348391132 70-71 20.01685918359622 31.016733977224085 22.871185937286167 26.09522090189353 72-73 19.41747007422117 27.617875975808964 20.933224238788934 32.031429711180934 74-75 22.246435258114392 28.397241965681808 25.22165895531297 24.13466382089083 76-77 27.067648787450498 22.1282995853216 28.205944501196456 22.59810712603145 78-79 21.408862864081204 22.50986364152542 32.83775874770334 23.24351474669004 80-81 19.31622761936098 32.76950671601156 28.09706696793843 19.81719869668902 82-83 19.36776605626134 30.6285068703357 24.406332530653724 25.597394542749242 84-85 21.446512129003327 24.55914254017523 24.576438490185858 29.41790684063558 86-87 24.94168980170013 24.994321334607374 27.555887835642658 22.508101028049836 88-89 28.874536182651266 19.292052943531328 30.137797871696474 21.695613002120933 90-91 21.09215884459661 23.302037263161584 33.662983974152986 21.942819918088816 92-93 18.22046803888968 29.495039789420353 29.97414342826694 22.310348743423024 94-95 19.14128811150938 31.823354191356103 24.620086013865876 24.415271683268642 96-97 18.26658579644406 27.633622950060698 21.699037564761937 32.40075368873331 98-99 22.31899671842834 25.65752271913299 24.02451133117668 27.998969231261995 100-101 27.351202331285773 26.73614750833351 24.985033462150383 20.927616698230338 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6828.0 1 4196.5 2 1643.0 3 1613.0 4 1237.0 5 1199.5 6 2139.0 7 3725.0 8 4761.0 9 4509.0 10 3254.5 11 2189.0 12 1937.5 13 1837.0 14 1804.0 15 1693.5 16 1575.5 17 1601.5 18 1609.5 19 1731.5 20 1942.5 21 2172.0 22 2424.0 23 2816.5 24 3317.5 25 3875.0 26 4616.5 27 5513.0 28 6273.5 29 6951.5 30 7841.5 31 8941.5 32 10413.0 33 11785.0 34 13497.0 35 14709.5 36 15640.5 37 17042.0 38 16981.0 39 16127.0 40 15996.0 41 16301.0 42 16667.0 43 17413.5 44 21553.0 45 27470.0 46 50164.0 47 118478.0 48 433848.5 49 436506.5 50 136497.5 51 69419.0 52 26323.5 53 12903.0 54 9806.5 55 8415.0 56 7842.5 57 7640.0 58 9152.0 59 10043.5 60 10363.5 61 10698.0 62 8857.0 63 5202.5 64 2267.0 65 1622.0 66 1095.0 67 706.5 68 618.5 69 537.5 70 416.0 71 369.5 72 319.5 73 311.5 74 254.0 75 228.0 76 188.5 77 72.0 78 21.5 79 11.0 80 5.5 81 3.0 82 2.0 83 3.0 84 2.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3895374061316181 2 0.0 3 0.0 4 0.0 5 6.405907411343697E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013102992432293926 16-17 0.002212949833009641 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0022420675939702943 36-37 0.006959144869596107 38-39 0.011734457667143228 40-41 0.0022420675939702943 42-43 8.444150678589418E-4 44-45 4.076486534491444E-4 46-47 4.076486534491444E-4 48-49 2.3294208768522535E-4 50-51 6.114729801737166E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 4.076486534491444E-4 60-61 0.0020091255062850686 62-63 0.0 64-65 2.911776096065317E-4 66-67 0.0016597123747572307 68-69 0.0015141235699539647 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12235283155666461 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14055143215707283 88-89 0.4254396053961035 90-91 0.32349832427285674 92-93 0.0 94-95 0.0 96-97 6.114729801737166E-4 98-99 0.0 100-101 5.823552192130634E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1717165.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.982752117340887 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49318368411339 13.575752087402945 2 12.979486113912802 4.668137627939242 3 4.558431452771892 2.459194285772611 4 2.1775842462830206 1.566358308621366 5 1.1976516944170639 1.0768536771807677 6 0.7431288993138992 0.8018101672556535 7 0.4762667497946548 0.5995210832302222 8 0.3592305624975219 0.5167963326692696 9 0.24498753066115098 0.39649950321470356 >10 1.4251761560902345 4.860553029124338 >50 0.14034772248821936 1.790059971485811 >100 0.14650615660945152 5.460758001090911 >500 0.024633778544951624 3.0236513209579634 >1k 0.02171662055936525 8.412482850144018 >5k 0.004861929975977295 5.85885994850567 >10k+ 0.006806701966368212 44.93271180540451 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 137124 7.985487707937211 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 113185 6.591387548663058 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 70838 4.1252879018614985 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 50275 2.927790864593676 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 49821 2.901351937641403 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 45268 2.636205606333695 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 38339 2.2326916749409635 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 33966 1.9780277375790911 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 27517 1.6024668567085867 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 25669 1.4948476121980125 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 22681 1.320839872697149 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 22187 1.2920715248680237 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 22013 1.2819385440537165 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 19604 1.1416491717452895 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 19088 1.1115996424338954 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 13323 0.7758718585575644 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 12736 0.7416876071897576 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 12322 0.7175781011143367 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12093 0.7042421665943576 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11882 0.6919544714689618 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 10953 0.6378536716040684 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 9885 0.5756581341921131 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 8802 0.5125890639513384 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 8302 0.4834713029906852 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7139 0.41574339099620594 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 7092 0.41300632146590455 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7058 0.4110263137205802 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6379 0.37148439433601316 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6227 0.3626325950039746 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6143 0.3577408111625849 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 5955 0.34679253304137925 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 5834 0.3397460348889012 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5582 0.325070683364732 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5393 0.3140641697216051 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 5370 0.312724752717415 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5356 0.31190945541051673 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 4842 0.2819763971429653 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4516 0.26299161699661944 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 4456 0.25949748568134107 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 4352 0.2534409914015252 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4219 0.24569566698599146 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4114 0.2395809371842543 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4104 0.2389985819650412 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3534 0.20580433446989657 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3283 0.19118721846764872 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3222 0.18763485163044902 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 3196 0.18612072806049507 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 2978 0.17342538428165027 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2914 0.16969831087868667 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2843 0.16556358882227393 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2729 0.158924739323245 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 2725 0.15869179723555976 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 2698 0.15711943814368448 No Hit CAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTA 2667 0.155314136964124 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2663 0.1550811948764388 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2656 0.15467354622298962 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2646 0.15409119100377658 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2540 0.14791822568011812 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 2431 0.1415705537906957 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2371 0.13807642247541732 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2348 0.13673700547122727 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2305 0.1342328780286111 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2087 0.12153753424976634 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 2069 0.12048929485518281 No Hit AGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACA 2051 0.1194410554605993 No Hit CTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTA 2037 0.11862575815370102 No Hit ACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGAT 2012 0.11716987010566834 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1972 0.1148404492288161 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1957 0.11396691639999651 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 1914 0.11146278895738032 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1852 0.10785218659825933 No Hit CGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTG 1793 0.10441629080490226 No Hit CAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTAT 1731 0.10080568844578128 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1647104384261268E-4 0.0 10-11 0.0 0.0 0.0 2.0382432672457217E-4 0.0 12-13 0.0 0.0 0.0 5.532374582524102E-4 0.0 14-15 0.0 0.0 0.0 8.444150678589419E-4 0.0 16-17 0.0 0.0 0.0 0.0012520637213080863 0.0 18-19 0.0 0.0 0.0 0.0016597123747572307 0.0 20-21 0.0 0.0 0.0 0.002795305052222704 0.0 22-23 0.0 0.0 0.0 0.005124725929074958 0.0 24-25 0.0 0.0 0.0 0.007920030981297663 0.0 26-27 0.0 0.0 0.0 0.010802689316402325 0.0 28-29 0.0 0.0 0.0 0.021081258935512895 0.0 30-31 0.0 0.0 0.0 0.04757842140970728 0.0 32-33 0.0 0.0 0.0 0.09113859180684442 0.0 34-35 0.0 0.0 0.0 0.20009725332160858 0.0 36-37 0.0 0.0 0.0 0.3651367224465908 0.0 38-39 0.0 0.0 0.0 0.5185290871873116 0.0 40-41 0.0 0.0 0.0 0.6785603014270615 0.0 42-43 0.0 0.0 0.0 0.8431338863766731 0.0 44-45 0.0 0.0 0.0 1.0211598768901067 0.0 46-47 0.0 0.0 0.0 1.2776873509534612 0.0 48-49 0.0 0.0 0.0 1.6131821927421068 0.0 50-51 0.0 0.0 0.0 1.8997883138778162 0.0 52-53 0.0 0.0 0.0 2.129847743227937 0.0 54-55 0.0 0.0 0.0 2.3625277710645163 0.0 56-57 0.0 0.0 0.0 2.6028075345118262 0.0 58-59 0.0 0.0 0.0 3.051803408525098 0.0 60-61 0.0 0.0 0.0 3.8168143422443386 0.0 62-63 0.0 0.0 0.0 4.384057443518824 0.0 64-65 0.0 0.0 0.0 4.742846494075991 0.0 66-67 0.0 0.0 0.0 5.159346946857174 0.0 68-69 0.0 0.0 0.0 5.60895429385062 0.0 70-71 0.0 0.0 0.0 6.163734993433945 0.0 72-73 0.0 0.0 0.0 6.886903704652727 0.0 74-75 0.0 0.0 0.0 7.580925537149895 0.0 76-77 0.0 0.0 0.0 8.106646711294488 0.0 78-79 0.0 0.0 0.0 8.688827223941788 0.0 80-81 0.0 0.0 0.0 9.32903943418367 0.0 82-83 0.0 0.0 0.0 9.976152553773225 0.0 84-85 0.0 0.0 0.0 11.227051564642885 0.0 86-87 0.0 0.0 0.0 12.677378120332058 0.0 88-89 0.0 0.0 0.0 13.541272970273678 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTCAG 40 5.3155044E-4 47.632347 1 GCTTATG 425 0.0 43.709686 1 GTGTGCG 50 0.0016238454 37.962738 9 GTAAGCG 80 1.8189894E-12 35.58903 94-95 TATGTAC 515 0.0 33.169395 4 CTTATGT 530 0.0 32.230637 2 CGTCAGA 105 1.9178897E-6 31.633772 2 TTATGTA 525 0.0 31.63377 3 CGTCACT 140 1.661465E-8 30.620796 1 ACCACTC 910 0.0 29.461983 94-95 CCACTGT 1010 0.0 28.42424 94-95 TAGGACG 85 6.4475404E-4 27.912153 4 CATCGCA 95 4.0199666E-10 27.472235 92-93 CATTCCG 70 0.008432962 27.11624 9 ATGGGCG 145 7.963263E-7 26.179676 5 GCGTTGT 445 0.0 25.058945 94-95 TGTAGCC 420 0.0 24.855106 2 GTAGCCC 345 0.0 24.756866 3 ATACCAT 550 0.0 24.157406 94-95 GTGTAGC 355 0.0 24.151615 1 >>END_MODULE