FastQCFastQC Report
Fri 27 May 2016
SRR938985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364722
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161400.4796830169030309No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT149500.4443160534510726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122510.36410140273104286No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78500.23330307823350638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78070.23202511232725914No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61000.18129283786297948No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55890.16610584767478562No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT43890.13044168284928145No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35310.10494180499904598No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34510.10256419401067905No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33900.10075126563204924No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG154850.024.797541
TACATGG155300.024.2457522
ACATGGG159050.022.6318423
GAGTACT130100.021.52531212-13
AGTACTT134450.020.44153212-13
GTACTTT140100.019.90544114-15
CATGGGG71550.019.6601494
TTTGCGT7600.019.3191533
CATGGGA91850.019.0793044
GTATCAA357600.018.1178421
AGAGTAC244800.018.06265610-11
ACTTTTT165750.017.12804416-17
TACTTTT164250.016.64720214-15
GGACCGT3502.6093403E-816.2322526
AGTACAT115100.016.0071582
TATCAAC404450.015.949692
GTATAGG7750.015.9236621
CAACGCA402750.015.9111735
TCAACGC405400.015.8422154
ATCAACG406250.015.797413
Produced by FastQC (version 0.11.5)