##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938985_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3364722 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.663913987544884 33.0 31.0 34.0 30.0 34.0 2 31.740569949018077 33.0 31.0 34.0 30.0 34.0 3 31.779082491807642 33.0 31.0 34.0 30.0 34.0 4 35.32422708324789 37.0 35.0 37.0 33.0 37.0 5 35.36485718582397 37.0 35.0 37.0 33.0 37.0 6 35.44911526123109 37.0 35.0 37.0 33.0 37.0 7 35.36745383422464 37.0 35.0 37.0 33.0 37.0 8 35.3572488306612 37.0 35.0 37.0 33.0 37.0 9 36.92340020958641 39.0 37.0 39.0 33.0 39.0 10-11 36.802490517790176 39.0 37.0 39.0 32.0 39.0 12-13 36.683793193018616 39.0 37.0 39.0 32.0 39.0 14-15 37.83853361436695 40.0 38.0 41.0 32.0 41.0 16-17 37.74937216209838 40.0 38.0 41.0 32.0 41.0 18-19 37.68139923595471 40.0 37.0 41.0 32.0 41.0 20-21 37.800487083331106 40.0 38.0 41.0 32.0 41.0 22-23 37.72977514932883 40.0 38.0 41.0 32.0 41.0 24-25 37.635244457045786 40.0 37.5 41.0 32.0 41.0 26-27 37.5013761909602 40.0 37.0 41.0 32.0 41.0 28-29 37.36315124993982 40.0 37.0 41.0 31.0 41.0 30-31 37.15835171523828 40.0 37.0 41.0 31.0 41.0 32-33 36.93049247456402 40.0 36.0 41.0 30.5 41.0 34-35 36.58536544772495 39.0 35.5 41.0 30.0 41.0 36-37 36.29554417868698 39.0 35.0 41.0 30.0 41.0 38-39 36.03999676644906 39.0 35.0 41.0 29.5 41.0 40-41 35.88419474179442 39.0 35.0 41.0 28.5 41.0 42-43 35.655094982586974 39.0 35.0 40.0 27.0 41.0 44-45 35.5460067726249 39.0 35.0 41.0 26.5 41.0 46-47 35.286192737468355 39.0 35.0 41.0 25.5 41.0 48-49 35.00289236376735 39.0 35.0 41.0 24.0 41.0 50-51 33.87820078449275 38.0 33.0 40.0 23.0 40.5 52-53 33.98278475903804 38.0 33.5 39.5 23.0 40.5 54-55 34.465304414450884 38.0 34.0 40.0 23.0 41.0 56-57 34.34871439601845 38.0 34.0 40.0 22.5 41.0 58-59 34.11386839685419 38.0 34.0 40.0 22.0 41.0 60-61 33.775390507744774 38.0 33.5 40.0 21.5 41.0 62-63 33.38258227574225 37.0 33.0 40.0 19.5 41.0 64-65 32.981367257086916 36.5 32.5 39.5 18.0 41.0 66-67 32.545531250427224 36.0 32.0 39.0 15.5 41.0 68-69 32.08888238017881 35.5 31.5 39.0 12.5 40.0 70-71 31.62257818030732 35.0 31.0 38.0 8.0 40.0 72-73 31.17475678525596 35.0 31.0 37.0 7.0 39.5 74-75 30.705220669047847 35.0 30.5 37.0 4.0 39.0 76-77 30.276529977810945 35.0 30.0 36.0 2.0 39.0 78-79 29.863824856852958 34.0 29.5 36.0 2.0 38.5 80-81 29.396440627190003 34.0 29.0 35.5 2.0 37.0 82-83 28.919747010302785 34.0 29.0 35.0 2.0 37.0 84-85 28.493824304058403 34.0 28.5 35.0 2.0 36.0 86-87 28.040068986382828 34.0 27.5 35.0 2.0 36.0 88-89 27.542809480248295 34.0 26.5 35.0 2.0 35.5 90-91 27.05959704843372 33.0 25.5 35.0 2.0 35.0 92-93 26.522273162537648 33.0 25.0 35.0 2.0 35.0 94-95 25.94569952584493 33.0 23.5 35.0 2.0 35.0 96-97 25.256975910639866 32.0 19.0 35.0 2.0 35.0 98-99 24.351529190227303 32.0 4.5 34.5 2.0 35.0 100-101 21.146503039478446 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21710398292271194 1101 2 0.3171056538039103 1101 3 0.306976958695401 1101 4 0.22898736725284863 1101 5 0.1980765821227024 1101 6 0.2801823915568633 1101 7 0.36489866105513613 1101 8 0.2703251478957043 1101 9 0.3173760478785468 1101 10-11 0.288079233661378 1101 12-13 0.3220711873185991 1101 14-15 0.421972964429699 1101 16-17 0.25333313848912553 1101 18-19 0.44956146344156167 1101 20-21 0.4583890705613527 1101 22-23 0.5344458069684634 1101 24-25 0.5320892054450113 1101 26-27 0.438706616258159 1101 28-29 0.4405885739161519 1101 30-31 0.37397553441841325 1101 32-33 0.4330599093910408 1101 34-35 0.451966254804006 1101 36-37 0.37253856783579664 1101 38-39 0.5532486426344221 1101 40-41 0.3072266617807884 1101 42-43 0.26704669469273057 1101 44-45 0.392648591825683 1101 46-47 0.3772573873762539 1101 48-49 0.5003780130026954 1101 50-51 0.41404722385429693 1101 52-53 0.2702785303980022 1101 54-55 0.44020069687839936 1101 56-57 0.4668730662561913 1101 58-59 0.32438255747825906 1101 60-61 0.4364119932171775 1101 62-63 0.48577244593831637 1101 64-65 0.29875656151117624 1101 66-67 0.3436718505212113 1101 68-69 0.266800875002815 1101 70-71 0.18515812732731618 1101 72-73 0.20338469496785905 1101 74-75 0.1261938333386574 1101 76-77 0.08675180730272203 1101 78-79 -0.00309566244422399 1101 80-81 -0.016533865590240282 1101 82-83 0.08187382484201322 1101 84-85 0.029064191139941187 1101 86-87 -0.11827921975640976 1101 88-89 -0.21701707493004108 1101 90-91 -0.09905234947959585 1101 92-93 -0.04994738939682364 1101 94-95 -0.24148735706568658 1101 96-97 -0.3185260914467314 1101 98-99 -0.3142348468106384 1101 100-101 0.02247819130774431 1102 1 0.24795387708645578 1102 2 0.2410649137826688 1102 3 0.3151701735694985 1102 4 0.1820292703376154 1102 5 0.3238738356781212 1102 6 0.30138092725736243 1102 7 0.3969845814269135 1102 8 0.2772099793776377 1102 9 0.3339171827358882 1102 10-11 0.41908201643592946 1102 12-13 0.36847388986446816 1102 14-15 0.4032068713896635 1102 16-17 0.47289389909734325 1102 18-19 0.5659424941529991 1102 20-21 0.5435736091329275 1102 22-23 0.4939741228978889 1102 24-25 0.5654301243408781 1102 26-27 0.5538067633440633 1102 28-29 0.5266013348170304 1102 30-31 0.4376154943545316 1102 32-33 0.3757322812080659 1102 34-35 0.45143543362060257 1102 36-37 0.5350493217582866 1102 38-39 0.5986654911611708 1102 40-41 0.5268902059204379 1102 42-43 0.4083174866607848 1102 44-45 0.5783604195005339 1102 46-47 0.7728368377341255 1102 48-49 0.6788369664473564 1102 50-51 0.5980607264920081 1102 52-53 0.6863036283501458 1102 54-55 0.6560757823421213 1102 56-57 0.5825983915229287 1102 58-59 0.5531415611289177 1102 60-61 0.6097817742534346 1102 62-63 0.6467921070100004 1102 64-65 0.6488235448876907 1102 66-67 0.5341855411160381 1102 68-69 0.538582077777022 1102 70-71 0.5885880294130139 1102 72-73 0.482397983157842 1102 74-75 0.41681984408122474 1102 76-77 0.5101996220804423 1102 78-79 0.39302197600701305 1102 80-81 0.3198779951347923 1102 82-83 0.3418329536292255 1102 84-85 0.27036323974893506 1102 86-87 0.11439176445771793 1102 88-89 0.007668834845492967 1102 90-91 0.11421363885949276 1102 92-93 0.15782530611011936 1102 94-95 0.03828901180457933 1102 96-97 0.011506933502367644 1102 98-99 -0.07119428087048973 1102 100-101 -0.06832548306474351 1103 1 0.3207704018014965 1103 2 0.32023419141213694 1103 3 0.35281021215441655 1103 4 0.36451141733419234 1103 5 0.17647714630417966 1103 6 0.3091038935105104 1103 7 0.27849746923901364 1103 8 0.10200333319986044 1103 9 0.3581513044678246 1103 10-11 0.37588530064078185 1103 12-13 0.36004901704765047 1103 14-15 0.45906786597331717 1103 16-17 0.4857379417401688 1103 18-19 0.5066433538389887 1103 20-21 0.5450346017225272 1103 22-23 0.46413576579794125 1103 24-25 0.494311086870141 1103 26-27 0.5671218012435304 1103 28-29 0.4559558350201769 1103 30-31 0.4229078100230552 1103 32-33 0.483980979465521 1103 34-35 0.37770433661742686 1103 36-37 0.41910113721868214 1103 38-39 0.5688744429624251 1103 40-41 0.5949783330811798 1103 42-43 0.45881108949034655 1103 44-45 0.6318542242279435 1103 46-47 0.5655885557351965 1103 48-49 0.5184279862308543 1103 50-51 0.45811431665932645 1103 52-53 0.5324537855064335 1103 54-55 0.6167118353141348 1103 56-57 0.456832737585799 1103 58-59 0.45674262096794394 1103 60-61 0.6896629287102627 1103 62-63 0.565192926565679 1103 64-65 0.504538873918861 1103 66-67 0.5501570062213545 1103 68-69 0.5745600235537367 1103 70-71 0.48899207434898173 1103 72-73 0.4393709612506811 1103 74-75 0.4475451059875901 1103 76-77 0.42337368045135193 1103 78-79 0.27211797219836953 1103 80-81 0.46219294080932016 1103 82-83 0.492101328202061 1103 84-85 0.36604805977320964 1103 86-87 0.3921598212284003 1103 88-89 0.42055784337754787 1103 90-91 0.4822998218188168 1103 92-93 0.25432011035645274 1103 94-95 0.13646874076295745 1103 96-97 0.12607947090954852 1103 98-99 -0.06969634845392036 1103 100-101 0.05005100904753235 1104 1 0.1735221303864698 1104 2 0.20710428630903266 1104 3 0.2132117293790614 1104 4 0.27832694093108756 1104 5 0.1911861210518353 1104 6 0.20236267040829148 1104 7 0.1777320377306495 1104 8 0.18438412068893228 1104 9 0.2960065749656522 1104 10-11 0.37661725614426445 1104 12-13 0.31916155064296703 1104 14-15 0.3422120579110697 1104 16-17 0.43832905044635595 1104 18-19 0.5137916420116895 1104 20-21 0.4661559534210298 1104 22-23 0.47699194896463126 1104 24-25 0.44495552948432504 1104 26-27 0.4149815263525909 1104 28-29 0.4582360803495007 1104 30-31 0.5301393158833676 1104 32-33 0.5276358251270636 1104 34-35 0.575357070397672 1104 36-37 0.6007893984669224 1104 38-39 0.7162948176476718 1104 40-41 0.5654005312345305 1104 42-43 0.5340675986397159 1104 44-45 0.5774191111460496 1104 46-47 0.6499212245668744 1104 48-49 0.6219272422704307 1104 50-51 0.42561376044793064 1104 52-53 0.5971384404580675 1104 54-55 0.7739987261519516 1104 56-57 0.8154499502941164 1104 58-59 0.6268713284330261 1104 60-61 0.6655310785154853 1104 62-63 0.7091447659894854 1104 64-65 0.6712707312181863 1104 66-67 0.5601984792718468 1104 68-69 0.62106416726715 1104 70-71 0.5371851720533662 1104 72-73 0.612716199131544 1104 74-75 0.6415912479665877 1104 76-77 0.5127231743703184 1104 78-79 0.45840318165907235 1104 80-81 0.48948356005932325 1104 82-83 0.6167725829116542 1104 84-85 0.6336817323044777 1104 86-87 0.5480312143290931 1104 88-89 0.5806738108709908 1104 90-91 0.5309656869113049 1104 92-93 0.5178125294976823 1104 94-95 0.5213226984936483 1104 96-97 0.5137944856062262 1104 98-99 0.55845401961977 1104 100-101 0.4682583640662692 1105 1 0.1331687595772877 1105 2 0.17981348624164184 1105 3 0.19458833790200813 1105 4 0.21962258644027344 1105 5 0.1556031137931697 1105 6 0.19436403719304707 1105 7 0.18221509173525874 1105 8 0.17102256578143482 1105 9 0.1817118572565306 1105 10-11 0.29747595765129375 1105 12-13 0.2291879609109415 1105 14-15 0.3907166387756078 1105 16-17 0.4692405962182207 1105 18-19 0.34923635722928026 1105 20-21 0.40503300686088295 1105 22-23 0.25488895129243616 1105 24-25 0.3065084437494292 1105 26-27 0.25265751768679223 1105 28-29 0.3372294528671631 1105 30-31 0.3027330035208493 1105 32-33 0.3305077815987616 1105 34-35 0.4037188981875346 1105 36-37 0.4719201518358318 1105 38-39 0.4902493103593386 1105 40-41 0.6309161721403029 1105 42-43 0.5055898314344844 1105 44-45 0.7159044478710328 1105 46-47 0.6834805836017708 1105 48-49 0.7693941582487582 1105 50-51 0.7197605521817252 1105 52-53 0.6920510587763076 1105 54-55 0.7675466003982621 1105 56-57 0.7193802632546138 1105 58-59 0.5557069274810189 1105 60-61 0.7337043564497492 1105 62-63 0.7101352578184432 1105 64-65 0.697440483037596 1105 66-67 0.8013346665593275 1105 68-69 0.7451577825314786 1105 70-71 0.6161903615642323 1105 72-73 0.7151887507241135 1105 74-75 0.6231154502602223 1105 76-77 0.6288315869779595 1105 78-79 0.699922258509865 1105 80-81 0.6286319772229128 1105 82-83 0.7681730389811499 1105 84-85 0.7729045633048628 1105 86-87 0.6959389183917217 1105 88-89 0.7389352891341261 1105 90-91 0.8217246083852494 1105 92-93 0.8254072018845946 1105 94-95 0.6731558748207469 1105 96-97 0.6923568463472307 1105 98-99 0.7774851685494788 1105 100-101 0.868141428037017 1106 1 0.22894603626438936 1106 2 0.19924808126613414 1106 3 0.23766880433008453 1106 4 0.16088878281725272 1106 5 0.22276379150681436 1106 6 0.24684275807864253 1106 7 0.27687608550380816 1106 8 0.2579341232769963 1106 9 0.42257428487996407 1106 10-11 0.32110569301565306 1106 12-13 0.37053782563943827 1106 14-15 0.4008756316293258 1106 16-17 0.38506545353379806 1106 18-19 0.44891437199076023 1106 20-21 0.3766654821338946 1106 22-23 0.4192388780826306 1106 24-25 0.4227038323360546 1106 26-27 0.430423710615905 1106 28-29 0.4187026134851095 1106 30-31 0.3477026277717954 1106 32-33 0.501206251433338 1106 34-35 0.3510480159231619 1106 36-37 0.35176352652063514 1106 38-39 0.45898709052764985 1106 40-41 0.5963873927558225 1106 42-43 0.5547275187401084 1106 44-45 0.4879679990408974 1106 46-47 0.36245233633881213 1106 48-49 0.5498635285064992 1106 50-51 0.4363105658582924 1106 52-53 0.4787551003714299 1106 54-55 0.49419313149461175 1106 56-57 0.4122408244301923 1106 58-59 0.4820323467996772 1106 60-61 0.5663026495234362 1106 62-63 0.5985702688861068 1106 64-65 0.4992848185293042 1106 66-67 0.46515017324705354 1106 68-69 0.40017248195752586 1106 70-71 0.4529934949899719 1106 72-73 0.3935630102936791 1106 74-75 0.4325357251245485 1106 76-77 0.42978972751780375 1106 78-79 0.5262389891920165 1106 80-81 0.42252694585877393 1106 82-83 0.5434876112956601 1106 84-85 0.5702883642845187 1106 86-87 0.7676491316770573 1106 88-89 0.7061182630843064 1106 90-91 0.9535974692682281 1106 92-93 0.6964289901347094 1106 94-95 0.6416301456533624 1106 96-97 0.8282891853143859 1106 98-99 0.7437171275611796 1106 100-101 1.0046213809620745 1107 1 0.2583236175634056 1107 2 0.2422405663487659 1107 3 0.2119265113065154 1107 4 0.27075213880960547 1107 5 0.16790029537781948 1107 6 0.16977586834318714 1107 7 0.14952484414467193 1107 8 0.23234068052209267 1107 9 0.2529517231677332 1107 10-11 0.26866139553501966 1107 12-13 0.3662869242733606 1107 14-15 0.36385939051093175 1107 16-17 0.24032460212104212 1107 18-19 0.29440123543802343 1107 20-21 0.27204907565426595 1107 22-23 0.3212676883800114 1107 24-25 0.2809956816295198 1107 26-27 0.32893149790141507 1107 28-29 0.3484788835363304 1107 30-31 0.3512342059475628 1107 32-33 0.30118719823962437 1107 34-35 0.3943095903116429 1107 36-37 0.462921035437283 1107 38-39 0.5469138515242378 1107 40-41 0.27735635141119985 1107 42-43 0.3476979951831396 1107 44-45 -0.025163343489715828 1107 46-47 0.21528586334817845 1107 48-49 0.3339227067739685 1107 50-51 0.3498517362076754 1107 52-53 0.36513460010517207 1107 54-55 0.46103504981435606 1107 56-57 0.40651853321762843 1107 58-59 0.5373542065351131 1107 60-61 0.5129181788913968 1107 62-63 0.3397946157954479 1107 64-65 0.379465023473756 1107 66-67 0.29415472157946 1107 68-69 0.4765796065763652 1107 70-71 0.2947780111843805 1107 72-73 0.4088552868242381 1107 74-75 0.3676548843909231 1107 76-77 0.4307902672235464 1107 78-79 0.5069954881187115 1107 80-81 0.4615768751077347 1107 82-83 0.41673208542032825 1107 84-85 0.5353636625779394 1107 86-87 0.6207114137915255 1107 88-89 0.642206071986795 1107 90-91 0.6872145972719146 1107 92-93 0.46576843091045106 1107 94-95 0.5565988233168042 1107 96-97 0.6588642522026866 1107 98-99 0.7872939644830943 1107 100-101 0.8995875738950225 1108 1 0.23072270280986729 1108 2 0.25303731816091 1108 3 0.23078997805988166 1108 4 0.23153854607443236 1108 5 0.208054133574322 1108 6 0.23772328848697555 1108 7 0.2887327104340045 1108 8 0.33102729246117946 1108 9 0.42318658133660136 1108 10-11 0.4109108358211202 1108 12-13 0.38774206957730684 1108 14-15 0.4397864282020265 1108 16-17 0.48372810596700333 1108 18-19 0.5371292033994806 1108 20-21 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2316 52-53 -2.380071784400677 2316 54-55 -2.174089317988077 2316 56-57 -2.0525806788352057 2316 58-59 -1.8860811973165994 2316 60-61 -1.9413129679360495 2316 62-63 -2.228920364414943 2316 64-65 -2.23243121762183 2316 66-67 -2.1954918782388013 2316 68-69 -2.332538064463268 2316 70-71 -2.0956385390401273 2316 72-73 -2.2168044552470327 2316 74-75 -2.08906280768408 2316 76-77 -2.1996403575995167 2316 78-79 -2.1596983694336807 2316 80-81 -1.9920752687717318 2316 82-83 -1.8223580547527938 2316 84-85 -1.9532283274342 2316 86-87 -1.930210724747262 2316 88-89 -2.2112491229904236 2316 90-91 -2.331074651717085 2316 92-93 -2.4179024053453055 2316 94-95 -2.449346243465932 2316 96-97 -2.6177031249177496 2316 98-99 -2.5404246593312436 2316 100-101 -2.1665271594680995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10544.0 3 3205.0 4 3426.0 5 3759.0 6 4477.0 7 5579.0 8 6914.0 9 8547.0 10 10631.0 11 13481.0 12 17630.0 13 23987.0 14 21729.0 15 22349.0 16 27820.0 17 36612.0 18 31823.0 19 23735.0 20 23967.0 21 25827.0 22 28045.0 23 31049.0 24 34602.0 25 39487.0 26 46136.0 27 53095.0 28 61744.0 29 72381.0 30 85935.0 31 104123.0 32 127110.0 33 161341.0 34 215329.0 35 303395.0 36 452137.0 37 641012.0 38 510837.0 39 70914.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.118156584578536 19.003069099006396 14.282298070251448 22.596476246163625 2 15.279452666022161 20.83911653156838 39.97673830594184 23.904692496467614 3 19.85504879355579 24.99051736371109 29.919554519878133 25.23487932285498 4 12.783006013264167 16.408101067583473 37.51730018362293 33.29159273552943 5 13.528366515277025 37.722026240871394 33.940468879431 14.809138364420576 6 31.283532663289687 35.956679709501046 17.482661594651546 15.277126032557723 7 27.88178511846171 33.360285961536746 20.88854458781511 17.869384332186435 8 25.899875128655548 34.331263246577635 19.960947594776872 19.80791402998994 9 26.51412753487163 17.19809418277276 20.98284425466767 35.30493402768794 10-11 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29.123730091144807 21.959151215462892 36-37 22.59471680731672 25.927542831641563 29.468151474006486 22.00958888703523 38-39 22.18065090852242 26.499176595328823 29.381772333573185 21.938400162575576 40-41 22.325789634129045 26.50069075351699 29.12468825026327 22.048831362090695 42-43 21.994865623195402 26.609891149479477 28.687983195824764 22.70726003150036 44-45 22.297407593208046 26.405224092248407 28.661895379260894 22.63547293528265 46-47 22.67875639621084 26.832231406933417 28.22518067092862 22.26383152592713 48-49 22.608790597209925 26.818441783499768 28.183889078169226 22.388878541121073 50-51 22.04769946757466 27.127897836229508 28.503254042329658 22.321148653866175 52-53 22.515413843782486 27.377675018819225 27.71137278799393 22.395538349404358 54-55 22.12552246765879 26.970868157665567 28.01064357740571 22.89296579726993 56-57 22.406937072125544 27.131477375379813 27.79694953974333 22.664636012751313 58-59 22.8504304491071 26.79991502535878 27.7939332107083 22.55572131482582 60-61 22.50081254127993 26.81178798179072 28.509588882465493 22.177810594463857 62-63 22.246369280044345 26.816450104741783 28.464931065152555 22.472249550061317 64-65 22.051999787896193 27.245077735298928 28.18138161732141 22.52154085948347 66-67 22.22749876422855 26.958490478099023 27.753402610230143 23.060608147442284 68-69 21.95189108375861 26.951564644591414 28.119843362983772 22.976700908666206 70-71 22.473219933434905 26.792317076705235 27.864550575042003 22.86991241481786 72-73 22.368226268927387 26.55458682602529 28.1230622608519 22.954124644195428 74-75 21.95756497941886 27.4679780437944 27.64837206940001 22.92608490738673 76-77 22.210925159264445 26.766462478227478 27.729717967860818 23.292894394647263 78-79 22.250145806201534 26.815947928447002 27.86475216827932 23.06915409707214 80-81 22.31288634601282 26.88655416948701 27.665514040196932 23.135045444303234 82-83 22.17438650430432 26.815144726235303 27.980116774970288 23.03035199449009 84-85 22.10905230875285 26.836263236384738 27.723059572057036 23.33162488280537 86-87 21.985099404012 26.779257443278993 27.85312005122976 23.382523101479247 88-89 22.05183226618907 26.73302416218522 27.539221882769855 23.675921688855862 90-91 22.03094965069205 26.374180353763638 27.816596410741518 23.778273584802793 92-93 22.252607558813477 26.66066063733422 27.668117036608596 23.41861476724371 94-95 22.146093857489674 26.41480965593296 27.74459112898395 23.694505357593425 96-97 21.68837787906092 26.231644754295434 28.118509640089002 23.961467726554638 98-99 21.862883831624742 26.409372498341497 27.58072565816726 24.147018011866496 100-101 22.0521849622789 25.446561252094995 28.208294788821874 24.292958996804227 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3435.0 1 2089.0 2 873.5 3 1014.0 4 928.5 5 978.5 6 1612.0 7 3087.0 8 5021.5 9 5935.5 10 5415.0 11 4887.5 12 5073.0 13 5436.0 14 5825.0 15 6299.5 16 6691.5 17 6935.5 18 7124.5 19 7377.0 20 7996.0 21 8775.0 22 10058.5 23 11980.5 24 13832.0 25 16278.0 26 20481.5 27 25691.0 28 29043.5 29 32204.5 30 36785.5 31 42607.5 32 48349.0 33 54190.5 34 61159.5 35 67132.5 36 74341.5 37 80855.5 38 84121.5 39 86238.5 40 88070.5 41 90489.0 42 93637.0 43 98706.5 44 104662.5 45 108469.0 46 112688.0 47 127075.5 48 156256.0 49 158237.0 50 134275.5 51 122583.5 52 116772.0 53 114178.5 54 111468.5 55 105888.0 56 98385.5 57 89638.5 58 82559.5 59 75012.5 60 63955.0 61 52073.0 62 41872.5 63 32982.0 64 24937.0 65 18449.5 66 13124.0 67 9587.5 68 7030.5 69 4801.0 70 3312.5 71 2235.0 72 1553.5 73 1058.5 74 716.0 75 468.0 76 320.0 77 186.0 78 117.0 79 92.0 80 65.0 81 52.0 82 42.0 83 33.0 84 28.5 85 21.0 86 20.0 87 17.0 88 14.0 89 12.5 90 12.0 91 11.5 92 7.5 93 2.0 94 1.5 95 3.0 96 5.5 97 4.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3260001866424626 2 0.08832824821783196 3 0.09852225533045524 4 0.10360439881808958 5 0.11466028991399588 6 0.05813258866557178 7 0.06039131910452037 8 0.06125320308780339 9 0.06464129874622629 10-11 0.06195162631563618 12-13 0.06036159896716578 14-15 0.05841492997044035 16-17 0.05999009725023345 18-19 0.06799967426729459 20-21 0.06993148319534274 22-23 0.08581689661136938 24-25 0.09446545658155414 26-27 0.08953191378069274 28-29 0.08736234375380789 30-31 0.08147775655759971 32-33 0.07266573583196471 34-35 0.32507886238447037 36-37 0.5050194339978162 38-39 0.4792520749113894 40-41 0.4600974463863582 42-43 0.490575447243487 44-45 0.5007397342187556 46-47 0.49472140640445184 48-49 0.5125980690232358 50-51 0.5087047310297849 52-53 0.5076793862910517 54-55 0.49892680584012583 56-57 0.47691904412905434 58-59 0.460290627279163 60-61 0.4197374998588294 62-63 0.4900256247024271 64-65 0.5142921168524472 66-67 0.5237431205312059 68-69 0.5350664928633034 70-71 0.5326740218062591 72-73 0.5217221511910939 74-75 0.5232081580588234 76-77 0.4978866010327153 78-79 0.47846449127149293 80-81 0.4839181364760596 82-83 0.49255183637756705 84-85 0.49510776819006147 86-87 0.4953752494262527 88-89 0.5006654338753692 90-91 0.5146784786380569 92-93 0.5209940078259065 94-95 0.5273392571511109 96-97 0.5186163968375396 98-99 0.5221085129767036 100-101 0.501111235935688 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3364722.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.34026395730921 #Duplication Level Percentage of deduplicated Percentage of total 1 71.25247333871036 32.30605948788264 2 14.36350398520368 13.024901240819956 3 5.442468833857391 7.402889205195688 4 2.690392012691704 4.87932336016313 5 1.597568306538403 3.621708435414133 6 0.9855478860371648 2.6811000777295892 7 0.6576951947059462 2.087405161199503 8 0.47362494677527006 1.717942408284582 9 0.38121450695002795 1.5555929732522777 >10 1.942854640735693 16.07238671121324 >50 0.13691007273175818 4.261715719937922 >100 0.07027757542338094 5.4936801421986665 >500 0.0028990696901966097 0.8399010240649475 >1k 0.0021084143172420407 1.9437069240094589 >5k 2.635517896552551E-4 0.8169280945524816 >10k+ 1.976638422414413E-4 1.294759034081734 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16140 0.4796830169030309 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14950 0.4443160534510726 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12251 0.36410140273104286 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7850 0.23330307823350638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7807 0.23202511232725914 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6100 0.18129283786297948 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5589 0.16610584767478562 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4389 0.13044168284928145 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3531 0.10494180499904598 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3451 0.10256419401067905 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3390 0.10075126563204924 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.9440274709173595E-5 0.0 0.0 0.0 0.0 3 5.9440274709173595E-5 0.0 0.0 0.0 0.0 4 5.9440274709173595E-5 0.0 0.0 0.0 0.0 5 8.91604120637604E-5 0.0 0.0 0.0 0.0 6 8.91604120637604E-5 0.0 0.0 0.0 0.0 7 1.48600686772934E-4 0.0 0.0 0.0 0.0 8 1.48600686772934E-4 0.0 0.0 2.9720137354586797E-5 0.0 9 1.48600686772934E-4 0.0 0.0 5.9440274709173595E-5 0.0 10-11 1.48600686772934E-4 0.0 0.0 7.4300343386467E-5 4.458020603188019E-5 12-13 1.48600686772934E-4 0.0 0.0 8.91604120637604E-5 8.91604120637604E-5 14-15 2.674812361912812E-4 0.0 0.0 8.91604120637604E-5 1.0402048074105379E-4 16-17 2.823413048685746E-4 0.0 0.0 1.48600686772934E-4 1.634607554502274E-4 18-19 4.012218542869218E-4 0.0 0.0 1.9318089280481417E-4 2.0804096148210757E-4 20-21 4.7552219767338876E-4 0.0 0.0 3.8636178560962835E-4 2.526211675139878E-4 22-23 4.7552219767338876E-4 0.0 0.0 6.984232278327898E-4 2.97201373545868E-4 24-25 5.94402747091736E-4 0.0 0.0 0.0010996450821197114 2.97201373545868E-4 26-27 5.94402747091736E-4 0.0 0.0 0.0017534881039206213 2.97201373545868E-4 28-29 7.4300343386467E-4 0.0 0.0 0.004621481358638247 2.97201373545868E-4 30-31 8.321638459284303E-4 0.0 0.0 0.011858334804480133 3.120614422231614E-4 32-33 8.470239146057238E-4 0.0 0.0 0.025930819841876982 3.269215109004548E-4 34-35 9.213242579921908E-4 0.0 0.0 0.04841410375062189 3.566416482550416E-4 36-37 0.0010699249447651247 0.0 0.0 0.08387022761464394 3.566416482550416E-4 38-39 0.0011145051507970049 0.0 0.0 0.13809461821808755 3.566416482550416E-4 40-41 0.0011293652194742983 0.0 0.0 0.19949642199266387 3.566416482550416E-4 42-43 0.0012482457688926456 0.0 0.0 0.2652225057523326 3.566416482550416E-4 44-45 0.001456286730374753 0.0 0.0 0.34454555235172474 3.8636178560962835E-4 46-47 0.001634607554502274 0.0 0.0 0.43994719325994835 4.3094199164150855E-4 48-49 0.0017237679665660342 0.0 0.0 0.5452010597012176 5.052423350279756E-4 50-51 0.0017237679665660342 0.0 0.0 0.6631900049989271 5.052423350279756E-4 52-53 0.0017386280352433277 0.0 0.0 0.7783406771792736 5.349624723825624E-4 54-55 0.0017534881039206211 0.0 0.0 0.9070585920619891 5.498225410598557E-4 56-57 0.0017534881039206211 0.0 0.0 1.0685429583781363 6.538430218009096E-4 58-59 0.0017534881039206211 0.0 0.0 1.244114669800358 7.132832965100832E-4 60-61 0.0017534881039206211 0.0 0.0 1.4409362794311091 7.4300343386467E-4 62-63 0.0017534881039206211 0.0 0.0 1.6471643125345867 7.4300343386467E-4 64-65 0.0017832082412752078 0.0 0.0 1.8611790216249664 7.727235712192568E-4 66-67 0.0018129283786297947 0.0 0.0 2.10745493981375 7.727235712192568E-4 68-69 0.0018129283786297947 0.0 0.0 2.3937787430878394 7.727235712192568E-4 70-71 0.0018129283786297947 0.0 0.0 2.7080543355439173 7.875836398965501E-4 72-73 0.0018129283786297947 0.0 0.0 3.031231109137694 8.321638459284303E-4 74-75 0.0018129283786297947 0.0 0.0 3.3698474940871788 8.916041206376039E-4 76-77 0.0018129283786297947 0.0 0.0 3.7303824803356713 9.213242579921907E-4 78-79 0.0018426485159843816 0.0 0.0 4.152675912006995 9.213242579921907E-4 80-81 0.002199290164239423 0.0 0.0 4.6158642526782305 9.361843266694841E-4 82-83 0.002199290164239423 0.0 0.0 5.0793497947230115 0.0010550648760878313 84-85 0.002214150232916716 0.0 0.0 5.5563431391954525 0.0010699249447651247 86-87 0.0022290103015940098 0.0 0.0 6.056102108881506 0.0010699249447651247 88-89 0.0022290103015940098 0.0 0.0 6.603680779571091 0.0010699249447651247 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15485 0.0 24.79754 1 TACATGG 15530 0.0 24.245752 2 ACATGGG 15905 0.0 22.631842 3 GAGTACT 13010 0.0 21.525312 12-13 AGTACTT 13445 0.0 20.441532 12-13 GTACTTT 14010 0.0 19.905441 14-15 CATGGGG 7155 0.0 19.660149 4 TTTGCGT 760 0.0 19.319153 3 CATGGGA 9185 0.0 19.079304 4 GTATCAA 35760 0.0 18.117842 1 AGAGTAC 24480 0.0 18.062656 10-11 ACTTTTT 16575 0.0 17.128044 16-17 TACTTTT 16425 0.0 16.647202 14-15 GGACCGT 350 2.6093403E-8 16.232252 6 AGTACAT 11510 0.0 16.007158 2 TATCAAC 40445 0.0 15.94969 2 GTATAGG 775 0.0 15.923662 1 CAACGCA 40275 0.0 15.911173 5 TCAACGC 40540 0.0 15.842215 4 ATCAACG 40625 0.0 15.79741 3 >>END_MODULE