FastQCFastQC Report
Fri 27 May 2016
SRR938985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364722
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT160090.47578967890958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155450.46199953517705183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129700.38547018148899076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82230.24438868946676726No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT76520.22741849103729816No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58240.1730900799531135No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC52250.15528771767771601No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT44000.13076860436018192No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC38450.11427392812838623No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC37460.11133163453028215No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35150.1044662828013726No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34780.10336663771925289No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG34680.10306943634570702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG2001.9896106E-823.8245981
GTACATG155050.023.110091
TACATGG155050.022.6786462
GAGTACT132200.022.36452112-13
ACATGGG155900.021.21573
GTACTTT142850.020.6805514-15
AGTACTT136050.019.97008712-13
GTGTACG3707.2759576E-1219.3172441
TACGCCG1751.0018601E-418.9815065
GTACGCT1751.0018601E-418.9815063
TTTGCGT6100.018.6703363
CATGGGG70900.017.870464
ACTTTTT169450.017.46150816-17
CATGGGA89150.017.4591564
AGAGTAC256650.016.87308910-11
AGTACAT120600.016.6048832
GTATCAA376300.016.5499611
TTACGCC2304.3106662E-516.505664
GTATAGG9600.016.3794141
TACTTTT165450.016.30672314-15
Produced by FastQC (version 0.11.5)