FastQCFastQC Report
Fri 27 May 2016
SRR938984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3375759
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161130.47731487940934175No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT148730.4405823993952175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124110.36765065278652886No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT80300.23787243105920772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78950.2338733304125087No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT63320.1875726318140602No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56280.1667180625157187No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT43140.12779348288784834No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT36180.10717589733153345No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34050.10086620520007501No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT7450.025.4104753
GTACATG156500.024.5698221
TACATGG158600.023.7530292
TACCGTA1652.512148E-622.9447297
GAGTACT129150.022.81049512-13
ACATGGG162900.021.7024423
AGTACTT132350.021.27564612-13
GTACTTT138650.020.8215314-15
CTTTGCG10400.019.5678982
GTATAGG8600.019.2959021
CATGGGA91800.018.868944
GTATCAA365100.018.6092931
CATGGGG72500.018.5391334
AGAGTAC252150.017.86766210-11
ACTTTTT166000.017.86233316-17
TACTTTT157500.017.71356214-15
AGTACAT118850.017.4016862
CTATGCG1650.001501281917.2090579
TATCAAC409850.016.5012322
CAACGCA408900.016.4583055
Produced by FastQC (version 0.11.5)