##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938984_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3375759 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.667582608829598 33.0 31.0 34.0 30.0 34.0 2 31.726107521301135 33.0 31.0 34.0 30.0 34.0 3 31.767605744367415 33.0 31.0 34.0 30.0 34.0 4 35.31510069291084 37.0 35.0 37.0 33.0 37.0 5 35.3508002200394 37.0 35.0 37.0 33.0 37.0 6 35.43576185385272 37.0 35.0 37.0 33.0 37.0 7 35.3417986888282 37.0 35.0 37.0 33.0 37.0 8 35.326462878422305 37.0 35.0 37.0 33.0 37.0 9 36.89873951309913 39.0 37.0 39.0 33.0 39.0 10-11 36.77969635865593 39.0 37.0 39.0 32.0 39.0 12-13 36.645989835174845 39.0 37.0 39.0 32.0 39.0 14-15 37.80283826540935 40.0 38.0 41.0 32.0 41.0 16-17 37.73267967292689 40.0 38.0 41.0 32.0 41.0 18-19 37.692238693579725 40.0 37.5 41.0 32.0 41.0 20-21 37.570296043052835 40.0 37.0 41.0 32.0 41.0 22-23 37.64546713790884 40.0 37.0 41.0 32.0 41.0 24-25 37.56945563945767 40.0 37.0 41.0 32.0 41.0 26-27 37.46893528240612 40.0 37.0 41.0 32.0 41.0 28-29 37.33394786179937 40.0 37.0 41.0 31.0 41.0 30-31 37.139740277667926 40.0 37.0 41.0 31.0 41.0 32-33 36.92806240018911 40.0 36.0 41.0 30.0 41.0 34-35 36.58452987905831 39.0 35.5 41.0 30.0 41.0 36-37 36.296628995138576 39.0 35.0 41.0 30.0 41.0 38-39 36.073554865735375 39.0 35.0 41.0 29.0 41.0 40-41 35.91556654370173 39.0 35.0 41.0 28.5 41.0 42-43 35.68606304537735 39.0 35.0 40.5 27.0 41.0 44-45 35.57531861723541 39.0 35.0 41.0 26.5 41.0 46-47 35.30322721497595 39.0 35.0 41.0 25.5 41.0 48-49 35.07156909009204 39.0 35.0 41.0 24.0 41.0 50-51 33.88987098901313 38.0 33.5 40.0 23.0 40.5 52-53 34.03977075970174 38.0 33.5 39.5 23.0 40.5 54-55 34.527404651813114 38.0 34.0 40.0 23.0 41.0 56-57 34.40495633722668 38.0 34.0 40.0 23.0 41.0 58-59 34.18544066682485 38.0 34.0 40.0 22.0 41.0 60-61 33.889076945362504 38.0 34.0 40.0 22.0 41.0 62-63 33.523574846427124 37.0 33.0 40.0 20.5 41.0 64-65 33.098578571515326 36.5 33.0 39.5 18.5 41.0 66-67 32.69116649026189 36.0 32.0 39.0 17.5 41.0 68-69 32.28138516404755 35.5 32.0 39.0 14.0 40.5 70-71 31.840036714706233 35.0 31.5 38.5 12.0 40.0 72-73 31.397016641294595 35.0 31.0 37.5 8.0 40.0 74-75 30.925805426275986 35.0 31.0 37.0 7.0 39.0 76-77 30.51640016363727 35.0 30.5 36.5 2.0 39.0 78-79 30.083976966365192 34.5 30.0 36.0 2.0 38.5 80-81 29.63176770024163 34.0 29.5 35.5 2.0 37.0 82-83 29.237092013973747 34.0 29.0 35.0 2.0 37.0 84-85 28.878786815054035 34.0 29.0 35.0 2.0 36.5 86-87 28.482355967946766 34.0 29.0 35.0 2.0 36.0 88-89 28.044727719010748 34.0 27.5 35.0 2.0 36.0 90-91 27.59141455299386 34.0 26.5 35.0 2.0 35.0 92-93 27.14436916260906 33.5 26.0 35.0 2.0 35.0 94-95 26.62980473428346 33.0 25.0 35.0 2.0 35.0 96-97 26.008839345462754 33.0 24.0 35.0 2.0 35.0 98-99 25.25795339655467 33.0 19.0 35.0 2.0 35.0 100-101 22.60928416394654 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33093203222986745 1101 2 0.36671584646058264 1101 3 0.35882384492531827 1101 4 0.35284866745486454 1101 5 0.05174829589950747 1101 6 0.21242224440459978 1101 7 0.27492064008682604 1101 8 0.34413498948764243 1101 9 0.3253051837046357 1101 10-11 0.4685114089684177 1101 12-13 0.4696616876797748 1101 14-15 0.15874893090289532 1101 16-17 0.3371265610676275 1101 18-19 0.39844277631006264 1101 20-21 0.4073292594979776 1101 22-23 0.4242883975889242 1101 24-25 0.501926135355447 1101 26-27 0.4416037447834782 1101 28-29 0.34050837743375695 1101 30-31 0.4103483852408374 1101 32-33 0.4301239423543137 1101 34-35 0.37630522153023804 1101 36-37 0.31672720238511687 1101 38-39 0.2779379962488946 1101 40-41 0.22458182986573405 1101 42-43 0.18244685901962043 1101 44-45 0.3702072420021594 1101 46-47 0.3353255351827471 1101 48-49 0.42478648515353257 1101 50-51 0.33758847761973243 1101 52-53 0.2630316960021375 1101 54-55 0.13457151183065008 1101 56-57 0.23777453493647016 1101 58-59 0.26960358870400114 1101 60-61 0.22681319385520737 1101 62-63 0.31061409772177484 1101 64-65 0.309006624080574 1101 66-67 0.30335333342713966 1101 68-69 0.15642768713502875 1101 70-71 0.12719938337515657 1101 72-73 -0.01073233162370002 1101 74-75 0.037902795060134054 1101 76-77 -0.06547502771658387 1101 78-79 -6.745428974959111E-4 1101 80-81 -0.002820808777173056 1101 82-83 -0.07817934494340051 1101 84-85 -0.2541247423162929 1101 86-87 -0.33902838758593035 1101 88-89 -0.24417195472460307 1101 90-91 -0.3893770813189512 1101 92-93 -0.1457183779785609 1101 94-95 -0.20085536958035632 1101 96-97 -0.38584855220724634 1101 98-99 -0.5176111098773291 1101 100-101 -0.26968292812339456 1102 1 0.23422134426918007 1102 2 0.23895709338933102 1102 3 0.25828791126438944 1102 4 0.25403340332709945 1102 5 0.23732492366363545 1102 6 0.32348566578051674 1102 7 0.3759610270647116 1102 8 0.2627667462444023 1102 9 0.3559271124515675 1102 10-11 0.25749674373499687 1102 12-13 0.38471351507534735 1102 14-15 0.42070071222968153 1102 16-17 0.4119495801093862 1102 18-19 0.5153803530913876 1102 20-21 0.5716593424218175 1102 22-23 0.5600354798985023 1102 24-25 0.5860385432178532 1102 26-27 0.5877727404837287 1102 28-29 0.594427680259308 1102 30-31 0.5379128815553358 1102 32-33 0.5683698729439897 1102 34-35 0.41050439229191227 1102 36-37 0.573011753982172 1102 38-39 0.5061631958803403 1102 40-41 0.5353339029615611 1102 42-43 0.45378630926532537 1102 44-45 0.46402404175645984 1102 46-47 0.48589832388053367 1102 48-49 0.4174557879790868 1102 50-51 0.3638411649661677 1102 52-53 0.41918533543702097 1102 54-55 0.46102536625325996 1102 56-57 0.42935354292172434 1102 58-59 0.30313975270892257 1102 60-61 0.2509298246905871 1102 62-63 0.2756102586554334 1102 64-65 0.2660509269061251 1102 66-67 0.2392873782674343 1102 68-69 0.21364167669276668 1102 70-71 0.09836569172896503 1102 72-73 0.0995041548627853 1102 74-75 0.06067920783654657 1102 76-77 0.04427658469127849 1102 78-79 0.035118992114789194 1102 80-81 -0.054925384944251476 1102 82-83 -0.027492152068703035 1102 84-85 -0.09072947204602144 1102 86-87 -0.03888096743850866 1102 88-89 0.006807391098494264 1102 90-91 0.020955381826009756 1102 92-93 0.14348271231627763 1102 94-95 0.08973507882259568 1102 96-97 -0.3126241203890636 1102 98-99 -0.47238614058985817 1102 100-101 -0.10475740601037486 1103 1 0.04619208401177488 1103 2 0.166792281885499 1103 3 0.25256413581779924 1103 4 0.2572336621243707 1103 5 0.15113862105941678 1103 6 0.18260776450816252 1103 7 0.18602091041883995 1103 8 0.12293810401703809 1103 9 0.18681314136531313 1103 10-11 0.2648714931140077 1103 12-13 0.31188692945558216 1103 14-15 0.2674580003515743 1103 16-17 0.348112654198907 1103 18-19 0.4190856239341798 1103 20-21 0.47643730663474315 1103 22-23 0.3809965342017847 1103 24-25 0.45918816703378695 1103 26-27 0.21455954930678445 1103 28-29 0.2091889382774994 1103 30-31 0.3575441665384389 1103 32-33 0.35530227581456586 1103 34-35 0.2183826849783017 1103 36-37 0.26383779102355476 1103 38-39 0.26790472827752865 1103 40-41 0.3237024417298855 1103 42-43 0.1304824305243386 1103 44-45 0.24896742704633112 1103 46-47 0.23171546055587555 1103 48-49 0.23943986049621202 1103 50-51 0.060480411445503535 1103 52-53 0.0712140463676505 1103 54-55 0.2840129049923945 1103 56-57 0.26141549785148754 1103 58-59 0.1540221369068675 1103 60-61 0.11790513529596325 1103 62-63 0.1184650534025522 1103 64-65 0.01580685443391161 1103 66-67 0.10936864906831545 1103 68-69 0.1506183568730748 1103 70-71 0.09977048830966595 1103 72-73 0.09955818010337936 1103 74-75 -0.030554408716902515 1103 76-77 -0.1305741653193877 1103 78-79 -0.015846689181383766 1103 80-81 -0.09345903981585835 1103 82-83 -0.183567423682355 1103 84-85 -0.17342359245336425 1103 86-87 -0.07589938971812771 1103 88-89 -0.2023267007648073 1103 90-91 -0.21314983109050445 1103 92-93 -0.06918495770746702 1103 94-95 -0.14344931947374562 1103 96-97 -0.2709234170412351 1103 98-99 -0.3288575879461675 1103 100-101 -0.038441399795644315 1104 1 0.19870852130499728 1104 2 0.1079367571160752 1104 3 0.16321493292292644 1104 4 0.21903665383618431 1104 5 0.2042929120324004 1104 6 0.24156649388080353 1104 7 0.20811663305300243 1104 8 0.2065861642856106 1104 9 0.33383238364477563 1104 10-11 0.2825775100608965 1104 12-13 0.3965127895205427 1104 14-15 0.3640072742132858 1104 16-17 0.2729025731149122 1104 18-19 0.35855188473567523 1104 20-21 0.3688238064150724 1104 22-23 0.34993855846890654 1104 24-25 0.382886551398542 1104 26-27 0.3253484600633385 1104 28-29 0.2694717772391968 1104 30-31 0.40608506587538074 1104 32-33 0.4015859391367158 1104 34-35 0.2571402073129434 1104 36-37 0.24608545964342454 1104 38-39 0.4009084298765515 1104 40-41 0.42312179731559496 1104 42-43 0.32776988169247545 1104 44-45 0.4596715678028218 1104 46-47 0.5657092152186607 1104 48-49 0.6215892294666148 1104 50-51 0.6234791634173931 1104 52-53 0.5472227596806718 1104 54-55 0.5940503823035641 1104 56-57 0.6059312900247633 1104 58-59 0.5314200826429456 1104 60-61 0.5424792088956139 1104 62-63 0.4520194591405087 1104 64-65 0.3849179810844632 1104 66-67 0.4724514234450936 1104 68-69 0.41195807661421924 1104 70-71 0.3623143377301403 1104 72-73 0.4135826189898921 1104 74-75 0.3611248842549841 1104 76-77 0.3452180189011962 1104 78-79 0.3436789908498845 1104 80-81 0.3147354056795777 1104 82-83 0.32922534302427664 1104 84-85 0.3111605390365959 1104 86-87 0.32701995123478156 1104 88-89 0.38718902439841685 1104 90-91 0.49961302269182184 1104 92-93 0.4763396229942032 1104 94-95 0.3936330882167134 1104 96-97 0.44844530629320545 1104 98-99 0.4034700596826859 1104 100-101 0.9834820112660054 1105 1 0.2700664184038857 1105 2 0.2593108256024159 1105 3 0.28849234909695554 1105 4 0.21258905780051407 1105 5 0.3118849913703059 1105 6 0.25774144881461325 1105 7 0.3818615667852754 1105 8 0.30413275468430356 1105 9 0.38620878918735 1105 10-11 0.3512164521746115 1105 12-13 0.540035458537929 1105 14-15 0.4921748844189935 1105 16-17 0.5358607312106543 1105 18-19 0.38846493810981997 1105 20-21 0.41811572403909736 1105 22-23 0.4103093302468537 1105 24-25 0.4465760161659347 1105 26-27 0.4332524709455754 1105 28-29 0.5334823419748744 1105 30-31 0.5359368834529974 1105 32-33 0.49430581809924945 1105 34-35 0.5524662018639717 1105 36-37 0.4241262637677181 1105 38-39 0.5935549882036071 1105 40-41 0.5381468998895755 1105 42-43 0.4579506209385755 1105 44-45 0.327931839737559 1105 46-47 0.42626787725664883 1105 48-49 0.3853394127458998 1105 50-51 0.3484255977031623 1105 52-53 0.28302815756351407 1105 54-55 0.3650205210678408 1105 56-57 0.3133527902999944 1105 58-59 0.236423277321407 1105 60-61 0.4223143485035976 1105 62-63 0.31394656196491866 1105 64-65 0.37919676859785767 1105 66-67 0.24994469963691301 1105 68-69 0.28211433046040924 1105 70-71 0.21484799779709363 1105 72-73 0.35370598780419016 1105 74-75 0.4004355721757449 1105 76-77 0.32087348986386743 1105 78-79 0.36870027086889223 1105 80-81 0.22676910481042967 1105 82-83 0.35747880463943815 1105 84-85 0.4373465032141013 1105 86-87 0.44167185079309235 1105 88-89 0.4369080139881518 1105 90-91 0.45404105038545595 1105 92-93 0.5005955001501619 1105 94-95 0.5028488169512322 1105 96-97 0.5620982222266058 1105 98-99 0.7397924211119431 1105 100-101 0.9082159842723101 1106 1 0.19191887433513344 1106 2 0.20076040387684913 1106 3 0.1617454956430251 1106 4 0.09411810525390507 1106 5 0.1008885470956784 1106 6 0.14642792622277767 1106 7 0.14418648936911893 1106 8 0.13370137704745133 1106 9 0.22592420284339454 1106 10-11 0.26573255131290807 1106 12-13 0.1815703300242646 1106 14-15 0.23476866774500849 1106 16-17 0.29586833738341767 1106 18-19 0.29266601195598696 1106 20-21 0.3904998635649619 1106 22-23 0.28783355309131764 1106 24-25 0.37584359947027224 1106 26-27 0.29646842779783356 1106 28-29 0.34453274944332435 1106 30-31 0.32195125605423414 1106 32-33 0.34785794355047983 1106 34-35 0.3073264535876561 1106 36-37 0.3392974775047293 1106 38-39 0.3956465787847776 1106 40-41 0.46059334302362487 1106 42-43 0.4595812788760796 1106 44-45 0.3937852922413967 1106 46-47 0.36496369307748466 1106 48-49 0.2886732973544852 1106 50-51 0.2223978376675788 1106 52-53 0.12678991968634534 1106 54-55 -0.049796759700448945 1106 56-57 0.129502256228335 1106 58-59 0.17636569994802187 1106 60-61 0.2699150180178833 1106 62-63 0.038420616860527446 1106 64-65 -0.01703778381416754 1106 66-67 0.08380115041278202 1106 68-69 0.15201455914459672 1106 70-71 0.21151307954740162 1106 72-73 0.08386096981170965 1106 74-75 0.18079753190223613 1106 76-77 0.17469168879059183 1106 78-79 0.2432287166718794 1106 80-81 0.2701950405051221 1106 82-83 0.24285085840588394 1106 84-85 0.2704296835210265 1106 86-87 0.3062228085863197 1106 88-89 0.5036876445576937 1106 90-91 0.41202692585808265 1106 92-93 0.5352452882893814 1106 94-95 0.5517687332904551 1106 96-97 0.6636019561659623 1106 98-99 0.5109280647638208 1106 100-101 0.9713652178096197 1107 1 0.04983220890831319 1107 2 0.07330043126623664 1107 3 0.20873329722213896 1107 4 0.20058166215451223 1107 5 0.046839947842968854 1107 6 0.10190361955182681 1107 7 -0.010280699724717124 1107 8 0.160750861101306 1107 9 0.17349938276235122 1107 10-11 0.25797750879173265 1107 12-13 0.3504569247233462 1107 14-15 0.16764248213964805 1107 16-17 0.19083183933617676 1107 18-19 0.2298976128824961 1107 20-21 0.3114681361175329 1107 22-23 0.28522000830741945 1107 24-25 0.3658233673931335 1107 26-27 0.34163079867040835 1107 28-29 0.32612804027238695 1107 30-31 0.24934279042340535 1107 32-33 0.3059191793978897 1107 34-35 0.2740477863123374 1107 36-37 0.21311081545931643 1107 38-39 0.3278067756953007 1107 40-41 0.13000898681508488 1107 42-43 0.24367606249430196 1107 44-45 0.2702031931211266 1107 46-47 0.295183456266372 1107 48-49 0.12007782054811145 1107 50-51 0.1679552694336337 1107 52-53 0.030781070265973653 1107 54-55 0.19148203155974386 1107 56-57 0.186385952780995 1107 58-59 0.22364602833886948 1107 60-61 0.12694073360785296 1107 62-63 0.034950449311885734 1107 64-65 0.17023361907468626 1107 66-67 0.1544735851939194 1107 68-69 0.21650148719391638 1107 70-71 0.31217030504771515 1107 72-73 0.19200765659773822 1107 74-75 0.2075317703251507 1107 76-77 0.26166848671215703 1107 78-79 0.36406430427565084 1107 80-81 0.339778204768173 1107 82-83 0.4653663706043041 1107 84-85 0.4080668065647437 1107 86-87 0.4264987031090044 1107 88-89 0.4539760502812875 1107 90-91 0.4614470500120227 1107 92-93 0.4050724421038865 1107 94-95 0.4623715886057447 1107 96-97 0.5652602890766225 1107 98-99 0.48220742663621863 1107 100-101 0.6065659676363211 1108 1 0.13459514797480665 1108 2 0.13897606480449198 1108 3 0.04688991473492976 1108 4 0.09405267546332396 1108 5 0.16380679166861967 1108 6 0.15630285410541234 1108 7 0.15402544894146075 1108 8 0.17981627537461975 1108 9 0.2458942967297162 1108 10-11 0.24994381868433635 1108 12-13 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6809.0 9 8428.0 10 10304.0 11 13066.0 12 16711.0 13 23247.0 14 22549.0 15 22040.0 16 27364.0 17 35604.0 18 30933.0 19 22798.0 20 22880.0 21 24621.0 22 26304.0 23 29344.0 24 32844.0 25 38042.0 26 43545.0 27 50191.0 28 59210.0 29 69925.0 30 82393.0 31 99643.0 32 123177.0 33 156229.0 34 208875.0 35 297110.0 36 446219.0 37 656073.0 38 552605.0 39 85009.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.119407702904354 18.965824054739027 14.314628792355263 22.600139450001354 2 15.27809651558791 20.844652231846624 40.004708424581295 23.872542827984173 3 19.857828814601632 24.992275883499566 29.91507326494995 25.234822036948852 4 12.817595966419649 16.426454683459017 37.48122455770025 33.27472479242108 5 13.52076721279194 37.74868786992163 33.93032854705133 14.800216370235095 6 31.273696506822102 35.93852101777127 17.502090214239548 15.28569226116708 7 27.820495713874827 33.36861862529689 20.898766793871626 17.912118866956657 8 25.90514967744652 34.326201684803415 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22.38318557365283 32-33 22.04373152292814 26.313797009444635 29.406163944499795 22.236307523127433 34-35 22.67130506361638 26.278394221710457 29.11439647707051 21.935904237602657 36-37 22.566602826014208 25.93687672297498 29.510562613257065 21.985957837753755 38-39 22.191849920939983 26.4998386621502 29.375853429554056 21.93245798735576 40-41 22.31666733431331 26.475485429989504 29.184949388165144 22.022897847532043 42-43 21.97419045580436 26.59522887041498 28.70851135805548 22.72206931572518 44-45 22.29248744500602 26.353143557139287 28.7128283603782 22.641540637476496 46-47 22.68225855900977 26.854406183921242 28.24506743552531 22.218267821543673 48-49 22.611565238394597 26.777981700042155 28.20917924359757 22.401273817965677 50-51 22.08532842170475 27.10071128125535 28.49705089224161 22.316909404798285 52-53 22.49993071607986 27.349536930117406 27.75746277218523 22.3930695816175 54-55 22.109719803723156 26.956287640685133 28.035464631909406 22.8985279236823 56-57 22.40319559034984 27.11079025594571 27.794570213115815 22.691443940588638 58-59 22.82972642502789 26.78483504928937 27.811459796289483 22.57397872939326 60-61 22.47201313831085 26.861552206342807 28.53253981095444 22.133894844391904 62-63 22.247233960906012 26.821328294637386 28.4731905146668 22.458247229789798 64-65 22.018689995557807 27.2024807138624 28.210743849067356 22.568085441512437 66-67 22.206530269885352 26.95407778962457 27.784706347699416 23.05468559279066 68-69 21.96209037543134 26.934988119242476 28.13865350023675 22.964268005089433 70-71 22.435881290501037 26.800592978183296 27.87484804077105 22.888677690544615 72-73 22.344715912875706 26.514266758801448 28.190456122301022 22.950561206021824 74-75 21.921075972304347 27.475868231064148 27.663375979934003 22.939679816697502 76-77 22.170288447742344 26.79904936906945 27.718213110643376 23.312449072544833 78-79 22.260654936752605 26.83234226880292 27.874166582257367 23.032836212187114 80-81 22.332547524971773 26.87379695281918 27.688923557732338 23.104731964476716 82-83 22.1971668921845 26.78774960712524 27.973457472173262 23.041626028516994 84-85 22.07792575420551 26.83015421563968 27.76642833837155 23.32549169178326 86-87 21.99654653632431 26.81113552096398 27.885579547128785 23.306738395582926 88-89 22.06202220031416 26.705738639521197 27.581587711473688 23.650651448690958 90-91 22.01957325202473 26.39194217520679 27.841779030003906 23.746705542764577 92-93 22.225623277284477 26.667412779330274 27.749415230474302 23.357548712910948 94-95 22.1390347236607 26.441469299193344 27.76079255962005 23.658703417525903 96-97 21.661057386033004 26.251641990550777 28.184060900619244 23.903239722796975 98-99 21.848479018522223 26.41898637199046 27.575450001199638 24.15708460828768 100-101 21.97241002626517 25.64129458422621 28.11842076445661 24.267874625052016 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4358.0 1 2595.0 2 961.5 3 1057.0 4 949.5 5 1034.5 6 1654.0 7 3137.0 8 5099.0 9 6112.0 10 5602.5 11 5052.5 12 5227.5 13 5552.5 14 5970.0 15 6349.5 16 6846.0 17 7184.5 18 7224.5 19 7431.5 20 7929.0 21 8828.5 22 10218.5 23 11963.0 24 13849.0 25 16332.5 26 20692.0 27 25850.5 28 29095.5 29 32409.0 30 36846.0 31 42667.5 32 48645.5 33 54230.0 34 60983.5 35 67051.5 36 74244.0 37 81207.0 38 84433.0 39 86181.0 40 87914.0 41 90677.5 42 94211.5 43 98794.0 44 104875.5 45 109316.5 46 113699.5 47 127645.0 48 157641.0 49 159290.0 50 134275.0 51 123042.0 52 117582.0 53 114990.5 54 112303.0 55 106622.5 56 98534.0 57 89462.5 58 82233.0 59 74899.0 60 63999.5 61 51799.0 62 41444.0 63 32658.5 64 24619.0 65 18205.5 66 13044.5 67 9660.0 68 6897.5 69 4559.5 70 3224.5 71 2229.5 72 1531.0 73 989.0 74 641.5 75 427.5 76 295.5 77 186.0 78 109.0 79 73.0 80 52.5 81 45.0 82 33.5 83 23.5 84 19.5 85 14.0 86 14.0 87 13.0 88 15.5 89 17.0 90 9.5 91 6.5 92 6.0 93 2.0 94 1.5 95 3.5 96 3.0 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28669108191668896 2 0.09126836364799738 3 0.0949416116494098 4 0.10865704571919974 5 0.11141198172025905 6 0.08587698351689206 7 0.09624502223055616 8 0.09301611874544362 9 0.09799277732800238 10-11 0.09092769951883413 12-13 0.09597841552077623 14-15 0.10505785513717063 16-17 0.0929420613260603 18-19 0.10418397758844751 20-21 0.10605022455690706 22-23 0.11877328920696056 24-25 0.13121493566335748 26-27 0.13405874056767678 28-29 0.12647526082282531 30-31 0.11883253514246722 32-33 0.11385587655990846 34-35 0.3881497464718305 36-37 0.5926963388085464 38-39 0.576107476866684 40-41 0.5389750867878897 42-43 0.5667614305405095 44-45 0.5872309012580578 46-47 0.575929739060164 48-49 0.5977174318427352 50-51 0.5898969683558571 52-53 0.5925186010020265 54-55 0.5830688742887156 56-57 0.5556676291168889 58-59 0.5493283140176772 60-61 0.4955181930937605 62-63 0.572760081510558 64-65 0.60533053455534 66-67 0.61882379636698 68-69 0.6210307074646028 70-71 0.6170316068179037 72-73 0.6081447164919059 74-75 0.6052268541682034 76-77 0.5889786563555041 78-79 0.5657690611207731 80-81 0.5604221154412977 82-83 0.5749669926081808 84-85 0.5848758753216684 86-87 0.5752335993179608 88-89 0.5869939175160311 90-91 0.6017165324894342 92-93 0.6099665290087355 94-95 0.6088704792018624 96-97 0.6059822398459132 98-99 0.6094481270730523 100-101 0.586342212225458 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3375759.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.62591254824193 #Duplication Level Percentage of deduplicated Percentage of total 1 71.57874082910178 32.658453693818714 2 14.035192643820423 12.807369443293585 3 5.489923065086052 7.514482489925775 4 2.751256337504879 5.021143242111759 5 1.5182375364497573 3.463548653275745 6 0.9964130576561234 2.727735301832678 7 0.6637143573663679 2.119780125834734 8 0.4766482747242124 1.7398009999069846 9 0.36559461120666276 1.5012528983121334 >10 1.9142483351329886 15.875493553434644 >50 0.1373976756652659 4.31210173806328 >100 0.06743232048346146 5.412374918183735 >500 0.0027305017979914726 0.8181157400664101 >1k 0.002015370371636968 1.9139595396789038 >5k 2.60047789888641E-4 0.8271330765431419 >10k+ 1.950358424164808E-4 1.2872545857178674 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16113 0.47731487940934175 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14873 0.4405823993952175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12411 0.36765065278652886 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8030 0.23787243105920772 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7895 0.2338733304125087 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6332 0.1875726318140602 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5628 0.1667180625157187 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4314 0.12779348288784834 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3618 0.10717589733153345 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3405 0.10086620520007501 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.9622967753325994E-5 2 0.0 0.0 0.0 0.0 2.9622967753325994E-5 3 0.0 0.0 0.0 0.0 2.9622967753325994E-5 4 0.0 0.0 0.0 0.0 2.9622967753325994E-5 5 2.9622967753325994E-5 0.0 0.0 0.0 2.9622967753325994E-5 6 5.924593550665199E-5 0.0 0.0 0.0 2.9622967753325994E-5 7 8.886890325997798E-5 0.0 0.0 0.0 2.9622967753325994E-5 8 8.886890325997798E-5 0.0 0.0 0.0 2.9622967753325994E-5 9 8.886890325997798E-5 0.0 0.0 0.0 2.9622967753325994E-5 10-11 8.886890325997798E-5 0.0 0.0 2.9622967753325994E-5 2.9622967753325994E-5 12-13 1.9254929039661894E-4 0.0 0.0 7.405741938331498E-5 1.4811483876662996E-4 14-15 2.962296775332599E-4 0.0 0.0 8.886890325997798E-5 1.9254929039661894E-4 16-17 2.962296775332599E-4 0.0 0.0 1.3330335488996698E-4 2.2217225814994497E-4 18-19 3.999100646699009E-4 0.0 0.0 2.2217225814994497E-4 2.6660670977993395E-4 20-21 4.443445162998899E-4 0.0 0.0 4.147215485465639E-4 2.6660670977993395E-4 22-23 4.443445162998899E-4 0.0 0.0 6.961397422031608E-4 2.6660670977993395E-4 24-25 5.628363873131938E-4 0.0 0.0 0.0010960498068730618 2.6660670977993395E-4 26-27 5.776478711898568E-4 0.0 0.0 0.0017477550974462335 3.1104116140992294E-4 28-29 6.665167744498348E-4 0.0 0.0 0.004576748517888866 3.258526452865859E-4 30-31 7.109512260798238E-4 0.0 0.0 0.012441646456396917 3.702870969165749E-4 32-33 7.109512260798238E-4 0.0 0.0 0.028600975365836244 4.443445162998899E-4 34-35 7.850086454631389E-4 0.0 0.0 0.05286218595581023 4.739674840532159E-4 36-37 9.775579358597577E-4 0.0 0.0 0.09298649577769029 4.739674840532159E-4 38-39 0.0010219923874897467 0.0 0.0 0.15328404663958534 5.035904518065419E-4 40-41 0.001051615355243073 0.0 0.0 0.2199209126006922 5.184019356832049E-4 42-43 0.0011404842585030508 0.0 0.0 0.2886461977884085 5.332134195598679E-4 44-45 0.0013330335488996696 0.0 0.0 0.37126465485243465 5.332134195598679E-4 46-47 0.0014663369037896366 0.0 0.0 0.47259001605268625 5.332134195598679E-4 48-49 0.0015403943231729516 0.0 0.0 0.5799584626746164 5.628363873131938E-4 50-51 0.0015403943231729516 0.0 0.0 0.7020050898183194 6.813282583264978E-4 52-53 0.0015403943231729516 0.0 0.0 0.8220077321870429 7.405741938331499E-4 54-55 0.0015403943231729516 0.0 0.0 0.9552666526253799 8.590660648464537E-4 56-57 0.0016292632264329296 0.0 0.0 1.122399436689645 9.035005164764428E-4 58-59 0.0016292632264329296 0.0 0.0 1.300670456629161 9.479349681064318E-4 60-61 0.0016292632264329296 0.0 0.0 1.5003292592865782 9.627464519830948E-4 62-63 0.0016292632264329296 0.0 0.0 1.70831211588268 0.0010071809036130838 64-65 0.0016588861941862557 0.0 0.0 1.9256262073210795 0.0010219923874897467 66-67 0.0016885091619395817 0.0 0.0 2.170815511415359 0.0010664268391197358 68-69 0.0017181321296929075 0.0 0.0 2.4574325359126643 0.0010664268391197358 70-71 0.0018366240007062114 0.0 0.0 2.768266336548314 0.0010812383229963987 72-73 0.0019551158717195155 0.0 0.0 3.090119881188201 0.0011256727746263876 74-75 0.0019551158717195155 0.0 0.0 3.431435715642023 0.0011701072262563766 76-77 0.0019551158717195155 0.0 0.0 3.793532061974803 0.0011849187101330397 78-79 0.0019551158717195155 0.0 0.0 4.217421919041021 0.0012145416778863657 80-81 0.0021624766459927974 0.0 0.0 4.677170378572641 0.0012145416778863657 82-83 0.0021920996137461237 0.0 0.0 5.1472720653340485 0.0013034105811463436 84-85 0.0022513455492527753 0.0 0.0 5.636065844747803 0.0013034105811463436 86-87 0.0023254029686360905 0.0 0.0 6.152379361204399 0.0013034105811463436 88-89 0.0023402144525127536 2.9622967753325994E-5 0.0 6.702670421674059 0.0013034105811463436 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 745 0.0 25.410475 3 GTACATG 15650 0.0 24.569822 1 TACATGG 15860 0.0 23.753029 2 TACCGTA 165 2.512148E-6 22.944729 7 GAGTACT 12915 0.0 22.810495 12-13 ACATGGG 16290 0.0 21.702442 3 AGTACTT 13235 0.0 21.275646 12-13 GTACTTT 13865 0.0 20.82153 14-15 CTTTGCG 1040 0.0 19.567898 2 GTATAGG 860 0.0 19.295902 1 CATGGGA 9180 0.0 18.86894 4 GTATCAA 36510 0.0 18.609293 1 CATGGGG 7250 0.0 18.539133 4 AGAGTAC 25215 0.0 17.867662 10-11 ACTTTTT 16600 0.0 17.862333 16-17 TACTTTT 15750 0.0 17.713562 14-15 AGTACAT 11885 0.0 17.401686 2 CTATGCG 165 0.0015012819 17.209057 9 TATCAAC 40985 0.0 16.501232 2 CAACGCA 40890 0.0 16.458305 5 >>END_MODULE