FastQCFastQC Report
Fri 27 May 2016
SRR938984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3375759
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT161340.4779369617321616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155630.46102224714501244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128010.379203610210326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83160.24634459983665893No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT75330.2231498160858047No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT59140.17519023129316993No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC53760.15925307464188054No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT44640.13223692805084722No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC37670.11158971952677903No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37130.10999007926809942No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT37070.10981234146157945No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG34750.10293981294280782No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA34730.10288056700730117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG152850.024.223511
TACATGG153250.023.7796292
GTACGCT2554.984031E-1022.3298263
ACATGGG158200.022.3157143
GTGTACG3650.022.1941011
GAGTACT137100.022.18626612-13
TGTACGC2406.009941E-921.7483232
TACGCTC2457.621566E-921.3044784
AGTACTT142250.021.24960112-13
TTTGCGT6600.020.130683
GTACTTT150500.020.0059314-15
CATGGGG69950.019.4009344
CATGGGA91150.018.7929444
AGTACAT116850.017.6240332
TACTTTT173100.017.42136214-15
AGAGTAC262350.017.35498810-11
ACTTTTT177600.017.07307816-17
GTATCAA396150.016.3110351
GTATAGA7050.016.2219721
GGGCGTA4409.822543E-1116.1761979
Produced by FastQC (version 0.11.5)