##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938984_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3375759 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.159091925697304 33.0 31.0 34.0 30.0 34.0 2 32.38578405626705 34.0 31.0 34.0 30.0 34.0 3 32.47866153952341 34.0 31.0 34.0 30.0 34.0 4 36.05254166544472 37.0 35.0 37.0 35.0 37.0 5 35.97082611643781 37.0 35.0 37.0 35.0 37.0 6 36.01629974177659 37.0 35.0 37.0 35.0 37.0 7 35.95583956082173 37.0 35.0 37.0 35.0 37.0 8 35.92650837930077 37.0 35.0 37.0 35.0 37.0 9 37.59664626532877 39.0 37.0 39.0 35.0 39.0 10-11 37.52243110364218 39.0 37.0 39.0 35.0 39.0 12-13 37.44917439307723 39.0 37.0 39.0 34.0 39.0 14-15 38.687940697188395 40.0 38.0 41.0 34.0 41.0 16-17 38.68499543361952 40.0 38.0 41.0 34.0 41.0 18-19 38.80042710394907 40.0 38.0 41.0 34.0 41.0 20-21 38.80194157817546 40.0 38.0 41.0 34.0 41.0 22-23 38.73910607955129 40.0 38.0 41.0 34.0 41.0 24-25 38.67507870081958 40.0 38.0 41.0 34.0 41.0 26-27 38.511803271501314 40.0 38.0 41.0 34.0 41.0 28-29 38.3298040529552 40.0 38.0 41.0 34.0 41.0 30-31 38.159021126804376 40.0 37.5 41.0 33.5 41.0 32-33 37.99091552447909 40.0 37.0 41.0 33.0 41.0 34-35 37.94759682192953 40.0 37.0 41.0 33.0 41.0 36-37 37.83504198611334 40.0 37.0 41.0 33.0 41.0 38-39 37.702470022297206 40.0 37.0 41.0 33.0 41.0 40-41 37.546289145641026 40.0 37.0 41.0 32.5 41.0 42-43 37.36174220375329 40.0 37.0 41.0 32.0 41.0 44-45 37.08410952914589 40.0 36.0 41.0 31.0 41.0 46-47 36.74194292305819 40.0 36.0 41.0 30.5 41.0 48-49 36.41505421447444 39.5 36.0 41.0 30.0 41.0 50-51 36.13066691075992 39.0 35.0 41.0 29.5 41.0 52-53 35.90139506404337 39.0 35.0 40.5 29.0 41.0 54-55 35.643384791390616 39.0 35.0 40.0 28.0 41.0 56-57 35.35393758855416 38.0 35.0 40.0 28.0 41.0 58-59 35.037374261610495 38.0 34.0 40.0 27.0 41.0 60-61 34.60663631497391 37.5 34.0 40.0 26.0 41.0 62-63 34.29303602537978 37.0 34.0 40.0 26.0 41.0 64-65 34.03163288611539 37.0 33.5 39.5 26.0 41.0 66-67 33.57336957407208 36.0 33.0 39.0 25.0 40.5 68-69 33.144121218368966 35.5 33.0 39.0 24.0 40.0 70-71 32.68046993283585 35.0 32.0 38.0 23.0 40.0 72-73 32.161899886810644 35.0 31.5 37.0 22.0 39.5 74-75 31.70064450690941 35.0 31.0 37.0 20.5 39.0 76-77 29.74116028425015 33.0 28.5 35.0 18.0 37.0 78-79 30.72078323719199 34.0 30.5 36.0 18.0 37.5 80-81 30.725423971320225 34.0 31.0 35.0 18.0 37.0 82-83 30.415077764733798 34.0 30.5 35.0 17.0 37.0 84-85 30.022169532836912 34.0 30.0 35.0 10.5 36.0 86-87 29.471150487934715 34.0 29.5 35.0 6.0 36.0 88-89 28.908953068035956 34.0 29.0 35.0 2.0 35.5 90-91 28.45335345325303 33.5 29.0 35.0 2.0 35.0 92-93 28.10416457454457 33.0 28.0 35.0 2.0 35.0 94-95 27.71082458789268 33.0 27.0 35.0 2.0 35.0 96-97 27.08825733709071 33.0 26.5 35.0 2.0 35.0 98-99 26.2006887932462 33.0 25.0 34.0 2.0 35.0 100-101 21.89324608184411 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10876689364435066 1101 2 0.08645372370662585 1101 3 0.12611485001318812 1101 4 0.12064680149119766 1101 5 0.13784479416722206 1101 6 0.11970693726296844 1101 7 0.0958321317065085 1101 8 0.16019946275513064 1101 9 0.23282221079536214 1101 10-11 0.18358813795843787 1101 12-13 0.25111371582463704 1101 14-15 0.245587654786398 1101 16-17 0.20824325144592848 1101 18-19 0.24365969806449073 1101 20-21 0.223345915694388 1101 22-23 0.296098161245105 1101 24-25 0.237401380165295 1101 26-27 0.2972896275776904 1101 28-29 0.27911074075958453 1101 30-31 0.1922898622079714 1101 32-33 0.29347440177781436 1101 34-35 0.3547480587217109 1101 36-37 0.28214382010386174 1101 38-39 0.29302376418333864 1101 40-41 0.3512633810550625 1101 42-43 0.25294181969245955 1101 44-45 0.27531255589674686 1101 46-47 0.3746863785939567 1101 48-49 0.17144257467514734 1101 50-51 0.2772523386058552 1101 52-53 0.4272775343885584 1101 54-55 0.28354818926962366 1101 56-57 0.12860004640545952 1101 58-59 0.24058629987472102 1101 60-61 0.3065845697367635 1101 62-63 0.21296912023976233 1101 64-65 0.07661376392152874 1101 66-67 0.1911384292888627 1101 68-69 0.3134457699823088 1101 70-71 0.3137736516171259 1101 72-73 0.198462030571406 1101 74-75 0.12683994691999345 1101 76-77 0.02378195744183742 1101 78-79 0.33599181272128575 1101 80-81 0.5268377111330835 1101 82-83 0.44523787745445986 1101 84-85 0.4683486773641832 1101 86-87 0.5336313940708983 1101 88-89 0.5722816454029278 1101 90-91 0.6306845305990407 1101 92-93 0.7001781635195456 1101 94-95 0.6349290650720576 1101 96-97 0.23312472501238446 1101 98-99 0.2249435600984775 1101 100-101 0.830585235061541 1102 1 0.11643043787039176 1102 2 0.0817493318147342 1102 3 0.06325628121712157 1102 4 0.13463175235114733 1102 5 0.11448714674707361 1102 6 0.09652281564135023 1102 7 0.10726871033058671 1102 8 0.16829682147749025 1102 9 0.19888639998454494 1102 10-11 0.22751127985032582 1102 12-13 0.14368857761825637 1102 14-15 0.2568629926242423 1102 16-17 0.2679000389152151 1102 18-19 0.2182278031013496 1102 20-21 0.2628322486182242 1102 22-23 0.17531691124510473 1102 24-25 0.1821241995756111 1102 26-27 0.2042963075531148 1102 28-29 0.21947238080873177 1102 30-31 0.13533731306792163 1102 32-33 0.11298760816601572 1102 34-35 0.24028591191581228 1102 36-37 0.2242629847230333 1102 38-39 0.2562946487042197 1102 40-41 0.25733985525654646 1102 42-43 0.20423942411506602 1102 44-45 0.1989658114250119 1102 46-47 0.25790198055955926 1102 48-49 0.1662540765179017 1102 50-51 0.21423867152968512 1102 52-53 0.21205793979395793 1102 54-55 0.20914439627208026 1102 56-57 0.08982509247424986 1102 58-59 0.009854573830494928 1102 60-61 0.03679687010531296 1102 62-63 0.18697326643140855 1102 64-65 0.15030310667336977 1102 66-67 0.009104108035792535 1102 68-69 0.12718732403636324 1102 70-71 0.12056611169083453 1102 72-73 0.24714177565740414 1102 74-75 0.17110860478240042 1102 76-77 0.01957088557451314 1102 78-79 0.07651354183676418 1102 80-81 0.2226001497080219 1102 82-83 0.22306151074684522 1102 84-85 0.1397103941946476 1102 86-87 0.21624925340750778 1102 88-89 0.17730391198768913 1102 90-91 0.22939383649584855 1102 92-93 0.08334884840898127 1102 94-95 0.32371515229564807 1102 96-97 0.1646849215725794 1102 98-99 0.4026961706635923 1102 100-101 0.9413353886241929 1103 1 0.023544631231921187 1103 2 0.035132238022804074 1103 3 0.06081415324194239 1103 4 0.03703101562461342 1103 5 -0.028604432911670585 1103 6 -0.055614319971262205 1103 7 0.021638844316925088 1103 8 0.0032235641867472964 1103 9 -0.006408485214386417 1103 10-11 0.011792095847560802 1103 12-13 -0.012119654949039216 1103 14-15 0.10304129855735056 1103 16-17 -0.037102754493758994 1103 18-19 -0.009353218856006151 1103 20-21 0.041315665161342 1103 22-23 0.027034090654183274 1103 24-25 -0.00682236716945539 1103 26-27 -0.05752118760659641 1103 28-29 0.0784142550757636 1103 30-31 0.09001345724300336 1103 32-33 0.07479541152498825 1103 34-35 -0.027353820276154295 1103 36-37 0.009137423515376497 1103 38-39 0.14777894237401767 1103 40-41 0.1591283292731589 1103 42-43 0.19454724917768118 1103 44-45 0.046487357907111004 1103 46-47 0.1975713161510697 1103 48-49 0.16414480015489374 1103 50-51 0.12920167990953502 1103 52-53 0.0915291716106097 1103 54-55 0.22097760786542153 1103 56-57 0.061937076180051065 1103 58-59 0.04615237891827917 1103 60-61 -0.03059117462814953 1103 62-63 -0.09647850769200517 1103 64-65 0.013727017805173602 1103 66-67 0.08566863222824139 1103 68-69 0.10138486912943279 1103 70-71 0.0705974884279712 1103 72-73 0.12113512872858223 1103 74-75 0.053681774135952764 1103 76-77 -0.13659255710278728 1103 78-79 0.09157259820712227 1103 80-81 0.08217096197073204 1103 82-83 0.10798798216052319 1103 84-85 0.053248492700763705 1103 86-87 -0.005598778408540284 1103 88-89 0.2853383960882745 1103 90-91 0.3959878926154907 1103 92-93 0.1953867665503104 1103 94-95 0.09119762660114716 1103 96-97 -0.0010726554323312598 1103 98-99 0.19155612863335492 1103 100-101 0.7732281112418669 1104 1 0.015559676493793972 1104 2 0.027255411075678637 1104 3 0.0404255915576357 1104 4 0.027755638665695415 1104 5 0.04632812625342808 1104 6 -4.2784648440630235E-4 1104 7 -0.023734895381195997 1104 8 0.061370332255279436 1104 9 0.10873193093902955 1104 10-11 0.052657699841113015 1104 12-13 0.04760882395095223 1104 14-15 0.06280695664213454 1104 16-17 0.0775760672196455 1104 18-19 0.10729825126713166 1104 20-21 0.10867225885511544 1104 22-23 0.10507222402570449 1104 24-25 0.09443716660647539 1104 26-27 0.05400884525203509 1104 28-29 0.08819681154677994 1104 30-31 0.004628514557566632 1104 32-33 0.07236189803641935 1104 34-35 0.17042763631824442 1104 36-37 0.07919260789194738 1104 38-39 0.1629665208430211 1104 40-41 0.19562715687069243 1104 42-43 0.16371984273944662 1104 44-45 0.19955473638163568 1104 46-47 -0.004850062591316373 1104 48-49 -0.23655395518117928 1104 50-51 -0.2018376606458716 1104 52-53 -0.1877353734234788 1104 54-55 -0.08419074555528283 1104 56-57 -0.1747972803679616 1104 58-59 -0.20350931146919038 1104 60-61 -0.14455791193039857 1104 62-63 -0.06019862519573138 1104 64-65 -0.14347387825147706 1104 66-67 -0.1340808632133843 1104 68-69 -0.049509651298748736 1104 70-71 -0.13159828979262755 1104 72-73 -0.10133766450491066 1104 74-75 -0.11972297398691722 1104 76-77 -0.2311822654704656 1104 78-79 -0.04024785167559841 1104 80-81 0.10509300520671516 1104 82-83 0.2533990286816383 1104 84-85 0.23581018776525653 1104 86-87 0.12677945041630423 1104 88-89 0.1958508424996026 1104 90-91 0.36432687196390035 1104 92-93 0.15756970983801466 1104 94-95 0.4127860876700815 1104 96-97 0.37140966963974265 1104 98-99 0.22454276318138966 1104 100-101 0.8849741717571398 1105 1 0.007544083751618302 1105 2 0.04262215338481923 1105 3 0.042511229631784886 1105 4 0.07173366084757049 1105 5 0.09414805698009587 1105 6 0.02356756002816951 1105 7 0.0068925458900608305 1105 8 0.04854943295774916 1105 9 -0.024420786820847695 1105 10-11 -0.001835915974815805 1105 12-13 0.05901057965062506 1105 14-15 0.1302885636064275 1105 16-17 0.1102728253434222 1105 18-19 0.10790191349953204 1105 20-21 0.1781205730245503 1105 22-23 0.052775194916137025 1105 24-25 0.09520792068973094 1105 26-27 0.14418082990187742 1105 28-29 0.08858593444254126 1105 30-31 0.08152742180272554 1105 32-33 0.06623885350605718 1105 34-35 0.19221695770860237 1105 36-37 0.18263249710029328 1105 38-39 0.1850931920736869 1105 40-41 0.1657367124019089 1105 42-43 0.08327927499639287 1105 44-45 0.12453111966313912 1105 46-47 0.1923636729920517 1105 48-49 0.14757580620077704 1105 50-51 0.08550137019107495 1105 52-53 0.24437601472228465 1105 54-55 0.19187674260692233 1105 56-57 0.2889142225079624 1105 58-59 0.2000674530332418 1105 60-61 0.1498669371384338 1105 62-63 0.07072798495966737 1105 64-65 0.17942253924930185 1105 66-67 0.2489358443663363 1105 68-69 0.24208440228266426 1105 70-71 0.22902637062785658 1105 72-73 0.1882148985690293 1105 74-75 0.19947980836218449 1105 76-77 0.0801152038661499 1105 78-79 0.30061617952700814 1105 80-81 0.08803295845132197 1105 82-83 0.1048912967035669 1105 84-85 0.03134238266811451 1105 86-87 0.07070883000653438 1105 88-89 0.19215537901436974 1105 90-91 0.3872187230901041 1105 92-93 0.43667455058748317 1105 94-95 0.32603268545346253 1105 96-97 0.3101986225094038 1105 98-99 0.49289089323316304 1105 100-101 1.068017073559755 1106 1 -0.03545110874799917 1106 2 -0.07001906337470842 1106 3 -0.09671559257053985 1106 4 -0.04708944252794112 1106 5 0.023569973593062343 1106 6 -0.03783642398104803 1106 7 0.004422143668229239 1106 8 -0.05465998700563546 1106 9 -0.06925015762569586 1106 10-11 0.02177369417852759 1106 12-13 0.04894042037009427 1106 14-15 0.012027547130905702 1106 16-17 -0.013588387310051075 1106 18-19 -0.07057101365799667 1106 20-21 -0.10448153645871372 1106 22-23 0.082613561962809 1106 24-25 -0.011688033710306911 1106 26-27 -0.039375294671117445 1106 28-29 0.03785625511365254 1106 30-31 0.09835296029409335 1106 32-33 -0.03747954559061384 1106 34-35 -0.06356608601512193 1106 36-37 -0.03545187367604541 1106 38-39 0.02264098906372425 1106 40-41 0.05167905091153102 1106 42-43 0.12658117375944045 1106 44-45 0.18401769943741897 1106 46-47 0.31442920156046483 1106 48-49 0.18504529596701502 1106 50-51 0.168994264443171 1106 52-53 0.1891286109435768 1106 54-55 0.15629863783421172 1106 56-57 0.2527873968839245 1106 58-59 0.1729502352814194 1106 60-61 0.23926922882767343 1106 62-63 0.08384382119670164 1106 64-65 0.0533646610507148 1106 66-67 0.17978899747067345 1106 68-69 0.06954475323589548 1106 70-71 0.061210104966413326 1106 72-73 0.21332970401638818 1106 74-75 0.2366313643841096 1106 76-77 0.08665351844662084 1106 78-79 0.14853442635764935 1106 80-81 0.192966598692891 1106 82-83 0.37086960592336027 1106 84-85 0.20487753501968342 1106 86-87 0.1752439837838189 1106 88-89 0.3491162745895302 1106 90-91 0.4999025329913884 1106 92-93 0.549924724524331 1106 94-95 0.5081721870816267 1106 96-97 0.3606187441511395 1106 98-99 0.5739791462228823 1106 100-101 0.7482990508510134 1107 1 -0.07561657219781637 1107 2 -0.03740117912022356 1107 3 -0.07156955811402099 1107 4 -0.08536836340868348 1107 5 -0.09940012632747397 1107 6 -0.014482182289441425 1107 7 -0.07265101022517939 1107 8 -0.02346440650281778 1107 9 -0.03314731217283651 1107 10-11 0.06530136675702636 1107 12-13 0.03910933567151886 1107 14-15 -0.025321134966240777 1107 16-17 -0.04452507894276181 1107 18-19 -0.07401458786012682 1107 20-21 -0.016793586661322024 1107 22-23 -0.020280850827923302 1107 24-25 -0.11056350499371348 1107 26-27 -0.13314378673326388 1107 28-29 -0.025774050170916496 1107 30-31 -0.0170832973915509 1107 32-33 0.15637855372127518 1107 34-35 -0.05165966359866303 1107 36-37 0.047349944956636136 1107 38-39 0.013852680555537233 1107 40-41 -0.10025384509334145 1107 42-43 -0.06891109550188901 1107 44-45 -0.12141815670631217 1107 46-47 -0.2098616190503293 1107 48-49 -0.1901234220857404 1107 50-51 -0.12037832982759511 1107 52-53 -0.070703178214508 1107 54-55 -0.14339692998832732 1107 56-57 -0.13903227838842014 1107 58-59 -0.14845043451868634 1107 60-61 -0.1484362267885544 1107 62-63 -0.21113239625022828 1107 64-65 -0.23838513183818577 1107 66-67 -0.05060571823764093 1107 68-69 -0.16361584662546136 1107 70-71 -0.0937773274759266 1107 72-73 -0.018188624611156 1107 74-75 -0.05830857339802975 1107 76-77 -0.34966897748160264 1107 78-79 0.031143645760156602 1107 80-81 0.1565958825111764 1107 82-83 0.1477049342860255 1107 84-85 -0.016018776993885098 1107 86-87 0.07275481132649375 1107 88-89 0.1913434092427373 1107 90-91 0.36099703059904087 1107 92-93 0.38866848301307044 1107 94-95 0.3606081728459074 1107 96-97 0.271864418769745 1107 98-99 0.2768925441974197 1107 100-101 0.7795277409508223 1108 1 0.01531409446030807 1108 2 0.03351580817323452 1108 3 0.028055674280338394 1108 4 0.01959833518717602 1108 5 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435.0 8 1036.0 9 1947.0 10 3367.0 11 5027.0 12 7348.0 13 10445.0 14 12490.0 15 16707.0 16 22386.0 17 31634.0 18 30934.0 19 20745.0 20 18213.0 21 19301.0 22 20955.0 23 22947.0 24 26439.0 25 31365.0 26 37662.0 27 45951.0 28 55746.0 29 68166.0 30 82792.0 31 101124.0 32 125156.0 33 159292.0 34 215339.0 35 310712.0 36 491245.0 37 767067.0 38 568706.0 39 42779.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.031125956838004 19.693395419670225 14.840905001063359 22.434573622428417 2 15.664536479055524 21.40760048332834 39.218765320628634 23.709097716987497 3 19.83103651652858 25.15144001689694 30.177746693410285 24.8397767731642 4 13.160240408157097 17.018394974285783 37.11716387336892 32.70420074418819 5 13.495788481465704 37.92659510116591 33.921934792410084 14.655681624958305 6 31.27696615783295 36.36850260933912 17.172049308022284 15.182481924805652 7 27.817803344373814 33.52049124359885 20.747363778042214 17.914341633985128 8 25.918822996546854 34.76305624897986 19.66893963698238 19.64918111749091 9 26.26707060545495 17.248713548567892 20.855872708922647 35.628343137054515 10-11 25.163363261417654 26.58725045241678 26.812399818825927 21.43698646733964 12-13 26.401307083829145 24.2054157302106 27.45018231455504 21.943094871405215 14-15 23.069020463552988 25.291486914304524 26.7203867394968 24.919105882645688 16-17 21.781605100854808 28.530310802049787 27.253734407352383 22.434349689743026 18-19 21.678058178916206 27.40628107634461 29.492952547856643 21.42270819688254 20-21 22.909988538873776 26.081749319190145 29.19713759187193 21.811124550064147 22-23 22.436791251982147 26.093050481388037 29.239142960146147 22.231015306483666 24-25 22.53553941498786 26.257961542870806 29.344304495670453 21.862194546470885 26-27 22.254150844299016 26.029375912202262 29.870363968517893 21.846109274980826 28-29 21.79219547366977 26.78199184242714 29.660425996050073 21.765386687853013 30-31 22.077849751715096 26.29921152546731 29.337743008313094 22.285195714504503 32-33 22.097178738174144 26.379000989110896 29.366995688969507 22.156824583745465 34-35 22.622851798737614 26.31580428554543 29.18406196334775 21.877281952369202 36-37 22.558669896216735 25.98878801811645 29.570809741738696 21.881732343928125 38-39 22.194489777749205 26.52585682168015 29.430865356455566 21.84878804411508 40-41 22.279560591259965 26.5637439650914 29.21432692300571 21.942368520642923 42-43 22.007041419066635 26.619630066296573 28.758953592397518 22.61437492223927 44-45 22.34564030116625 26.42785211613382 28.713422687476108 22.513084895223823 46-47 22.69991381170724 26.9087433498082 28.25100862816054 22.140334210324024 48-49 22.64186546934297 26.885173106665743 28.182543676947734 22.29041774704355 50-51 22.105281711315108 27.183852562235927 28.419373157257528 22.291492569191433 52-53 22.580684817843927 27.450700716490722 27.654536357601355 22.314078108063995 54-55 22.18560329691782 27.040185629365133 27.87999676517192 22.89421430854513 56-57 22.39737789338635 27.192921651101276 27.765192953643908 22.644507501868468 58-59 22.902725941575667 26.89696307577188 27.656497768940895 22.543813213711555 60-61 22.49605115194978 27.001648266357947 28.395179857734824 22.10712072395745 62-63 22.272131985725284 26.90879295589525 28.328458873989526 22.49061618438994 64-65 22.039276895611806 27.349133177940725 28.084110481506258 22.527479444941207 66-67 22.217668444318708 27.088751024785175 27.64171658830038 23.051863942595737 68-69 21.978258802367854 27.11162181833694 27.97191465738625 22.938204721908956 70-71 22.462415119088774 26.954604875525774 27.704954648717518 22.878025356667937 72-73 22.412307869134025 26.754753523577957 27.941345931389062 22.891592675898963 74-75 21.974213799030085 27.673243261737586 27.424765808222684 22.92777713100965 76-77 22.213891033889762 27.003890958256545 27.555790990719508 23.226427017134185 78-79 22.223389038871733 27.07544220088149 27.668390774038354 23.032777986208426 80-81 22.28432183695578 27.172363311480467 27.4518560122331 23.09145883933065 82-83 22.183485254723458 27.115309475587562 27.7368289620201 22.964376307668882 84-85 22.01219044368985 27.18567883548559 27.4839080633422 23.318222657482362 86-87 21.92813522635355 27.137609662703976 27.57843359612974 23.355821514812725 88-89 21.99826979967315 27.109948056797585 27.245611829631056 23.64617031389821 90-91 21.941003582908333 26.84850902385217 27.48046699675951 23.730020396479986 92-93 22.10332550398296 27.1746294685136 27.33872589838315 23.383319129120295 94-95 21.95571425566813 27.005349019287216 27.366497430651894 23.67243929439276 96-97 21.480840414623348 26.882651011030624 27.723089332897082 23.913419241448945 98-99 21.657129552198484 27.078310388863656 27.105548707712845 24.15901135122501 100-101 21.73412543303295 26.433135594764252 27.739288428494557 24.093450543708244 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1539.0 1 1068.5 2 726.5 3 887.0 4 829.0 5 876.0 6 1387.0 7 2737.5 8 4477.0 9 5366.5 10 4898.0 11 4252.0 12 4341.0 13 4671.0 14 4965.5 15 5232.5 16 5520.0 17 5838.0 18 6193.5 19 6651.0 20 7331.5 21 8379.0 22 9900.0 23 11716.0 24 13747.5 25 16443.0 26 20787.5 27 25839.0 28 29269.5 29 32724.5 30 37178.0 31 43051.0 32 49333.5 33 54679.5 34 60949.0 35 67601.5 36 74839.5 37 81406.5 38 85295.5 39 87337.0 40 88843.0 41 91419.5 42 94700.0 43 100135.5 44 106629.5 45 110431.5 46 114668.5 47 130386.0 48 162181.0 49 162440.5 50 135892.0 51 124571.0 52 118184.0 53 114934.5 54 112796.0 55 106855.5 56 98350.0 57 89243.5 58 81951.5 59 74599.5 60 63182.0 61 50863.0 62 40668.0 63 31802.0 64 23836.5 65 17390.0 66 12159.5 67 8937.0 68 6333.5 69 4083.0 70 2803.0 71 1778.5 72 1185.0 73 796.0 74 488.0 75 307.0 76 217.0 77 105.0 78 43.5 79 24.5 80 17.5 81 10.0 82 4.5 83 3.5 84 4.5 85 3.5 86 1.0 87 0.5 88 0.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4080267578343122 2 0.0 3 0.0 4 0.0 5 5.035904518065419E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014811483876662997 16-17 0.002651255613922676 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002547575226786035 36-37 0.0077908405191247365 38-39 0.013345146972873359 40-41 0.0025623867106626982 42-43 5.628363873131939E-4 44-45 4.591560001765529E-4 46-47 3.1104116140992294E-4 48-49 2.3698374202660793E-4 50-51 4.7396748405321586E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.3698374202660793E-4 60-61 0.0026956900655526654 62-63 0.0 64-65 2.5179522590327094E-4 66-67 0.0015255828392962887 68-69 0.0014219024521596477 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12514222727392565 78-79 7.405741938331498E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14887022444433978 88-89 0.4406860797823541 90-91 0.3392718496788426 92-93 0.0 94-95 0.0 96-97 6.072708389431829E-4 98-99 0.0 100-101 1.6292632264329295E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3375759.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.47658468091809 #Duplication Level Percentage of deduplicated Percentage of total 1 69.33320196322005 30.143708263531327 2 15.227288783847667 13.240610205434944 3 5.899773642114148 7.695060250488729 4 2.890672209055869 5.027062203271761 5 1.6411360001147597 3.567549414094627 6 1.041502538125062 2.716858397651122 7 0.7118984222972624 2.1665638428853238 8 0.5427342453288747 1.8876985101019996 9 0.3879566271905411 1.5180326239115634 >10 2.0885987364803458 16.579271540132723 >50 0.15308495418461146 4.57482079228819 >100 0.0759460965098485 5.731659829232797 >500 0.0034097700734748502 0.9448849837057466 >1k 0.002318643647727509 2.0822514780166963 >5k 2.72781605615001E-4 0.8046450642445552 >10k+ 2.0458620421125076E-4 1.3193226010079302 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16134 0.4779369617321616 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15563 0.46102224714501244 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12801 0.379203610210326 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8316 0.24634459983665893 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7533 0.2231498160858047 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5914 0.17519023129316993 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5376 0.15925307464188054 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4464 0.13223692805084722 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3767 0.11158971952677903 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3713 0.10999007926809942 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3707 0.10981234146157945 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3475 0.10293981294280782 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3473 0.10288056700730117 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.924593550665199E-5 0.0 0.0 0.0 0.0 3 5.924593550665199E-5 0.0 0.0 0.0 0.0 4 5.924593550665199E-5 0.0 0.0 0.0 0.0 5 1.1849187101330398E-4 0.0 0.0 0.0 0.0 6 1.1849187101330398E-4 0.0 0.0 0.0 2.9622967753325994E-5 7 2.6660670977993395E-4 0.0 0.0 0.0 2.9622967753325994E-5 8 2.6660670977993395E-4 0.0 0.0 0.0 2.9622967753325994E-5 9 2.6660670977993395E-4 0.0 0.0 0.0 2.9622967753325994E-5 10-11 2.6660670977993395E-4 0.0 0.0 0.0 5.924593550665199E-5 12-13 2.6660670977993395E-4 0.0 0.0 4.443445162998899E-5 1.9254929039661894E-4 14-15 4.147215485465639E-4 0.0 0.0 5.924593550665199E-5 2.3698374202660796E-4 16-17 4.147215485465639E-4 0.0 0.0 1.0368038713664098E-4 2.962296775332599E-4 18-19 4.887789679298789E-4 0.0 0.0 1.6292632264329297E-4 3.258526452865859E-4 20-21 5.628363873131938E-4 0.0 0.0 3.406641291632489E-4 4.295330324232269E-4 22-23 5.924593550665198E-4 0.0 0.0 6.517052905731719E-4 4.443445162998899E-4 24-25 6.813282583264978E-4 0.0 0.0 0.0010664268391197356 4.739674840532159E-4 26-27 6.961397422031608E-4 0.0 0.0 0.0017625665813228966 5.035904518065419E-4 28-29 7.701971615864758E-4 0.0 0.0 0.00469524038890217 5.035904518065419E-4 30-31 7.701971615864758E-4 0.0 0.0 0.012723064650053514 5.035904518065419E-4 32-33 7.850086454631388E-4 0.0 0.0 0.029134188785396115 5.035904518065419E-4 34-35 8.738775487231168E-4 0.0 0.0 0.05360276014964338 5.628363873131938E-4 36-37 9.183120003531058E-4 0.0 0.0 0.09428990635883663 5.628363873131938E-4 38-39 0.0010071809036130838 0.0 0.0 0.15568350702760475 5.924593550665198E-4 40-41 0.0010219923874897467 0.0 0.0 0.22343123427946132 5.924593550665198E-4 42-43 0.0011997301940097028 0.0 0.0 0.29298596256427073 5.924593550665198E-4 44-45 0.0014219024521596477 0.0 0.0 0.3769966991127033 6.220823228198459E-4 46-47 0.0015700172909262776 0.0 0.0 0.4795810364424712 6.665167744498348E-4 48-49 0.0015996402586796036 0.0 0.0 0.588149213258411 7.257627099564869E-4 50-51 0.0015996402586796036 0.0 0.0 0.7116621773059036 7.701971615864758E-4 52-53 0.0015996402586796036 0.0 0.0 0.8331015336106635 8.590660648464537E-4 54-55 0.0015996402586796036 0.0 0.0 0.9675305612752569 8.886890325997798E-4 56-57 0.0016292632264329296 0.0 0.0 1.1350632554041922 9.331234842297688E-4 58-59 0.0016292632264329296 0.0 0.0 1.3143710792150745 9.775579358597577E-4 60-61 0.0016736976780629188 0.0 0.0 1.515673956582801 9.775579358597577E-4 62-63 0.0017625665813228966 0.0 0.0 1.7249009778245425 0.0010071809036130838 64-65 0.0017773780651995595 0.0 0.0 1.9437258406183617 0.0010071809036130838 66-67 0.0018958699362128636 0.0 0.0 2.1910924328425105 0.0010071809036130838 68-69 0.0019847388394728417 0.0 0.0 2.4797534421147955 0.0010071809036130838 70-71 0.0020587962588561565 0.0 0.0 2.792764530880315 0.0010071809036130838 72-73 0.002103230710486146 0.0 0.0 3.118957840296064 0.0010368038713664098 74-75 0.0021328536782394716 0.0 0.0 3.4652355218485678 0.0010664268391197358 76-77 0.0021328536782394716 0.0 0.0 3.831567952570074 0.0010664268391197358 78-79 0.0021328536782394716 0.0 0.0 4.2590718117021975 0.0010664268391197358 80-81 0.0023254029686360905 0.0 0.0 4.72284899484827 0.0010812383229963987 82-83 0.0023402144525127536 0.0 0.0 5.194550321868356 0.0012145416778863657 84-85 0.0024290833557727314 0.0 0.0 5.686039791347664 0.0012145416778863657 86-87 0.0025179522590327093 0.0 0.0 6.206944867806025 0.0012589761295163546 88-89 0.0025179522590327093 0.0 0.0 6.7614572011805345 0.0013034105811463436 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15285 0.0 24.22351 1 TACATGG 15325 0.0 23.779629 2 GTACGCT 255 4.984031E-10 22.329826 3 ACATGGG 15820 0.0 22.315714 3 GTGTACG 365 0.0 22.194101 1 GAGTACT 13710 0.0 22.186266 12-13 TGTACGC 240 6.009941E-9 21.748323 2 TACGCTC 245 7.621566E-9 21.304478 4 AGTACTT 14225 0.0 21.249601 12-13 TTTGCGT 660 0.0 20.13068 3 GTACTTT 15050 0.0 20.00593 14-15 CATGGGG 6995 0.0 19.400934 4 CATGGGA 9115 0.0 18.792944 4 AGTACAT 11685 0.0 17.624033 2 TACTTTT 17310 0.0 17.421362 14-15 AGAGTAC 26235 0.0 17.354988 10-11 ACTTTTT 17760 0.0 17.073078 16-17 GTATCAA 39615 0.0 16.311035 1 GTATAGA 705 0.0 16.221972 1 GGGCGTA 440 9.822543E-11 16.176197 9 >>END_MODULE