FastQCFastQC Report
Fri 27 May 2016
SRR938983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225956
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT140650.43599478728166163No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74490.23090829509143954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62280.19305904978245209No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT59330.1839144737249981No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56700.17576185168055608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48100.14910308758085974No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41140.1275280877978497No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35140.1089289500538755No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88350.021.7556651
TACATGG85900.021.615242
TAACCCG1656.466196E-520.0946875
ACATGGG88400.020.039483
AGAGTAC108100.019.09482610-11
GAGTACT70200.018.71504612-13
GAGTACA71700.017.8718091
CATGGGG62350.017.7005424
GTACTTT74850.017.52130514-15
AGTACTT75400.017.42435512-13
TTTGCGT5600.016.9164453
GTATAGG9000.016.8745171
GTGTAGC19200.016.8086031
AGTACAT68700.016.547092
GTATTAG10600.016.1183471
GTCTTAG14400.015.1606991
ACTTTTT92600.014.62426316-17
TACTTTT88750.014.43036714-15
TATACAG19150.014.0985095
TATACTG15350.013.8858125
Produced by FastQC (version 0.11.5)