##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938983_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3225956 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.585614000934918 33.0 31.0 34.0 30.0 34.0 2 31.669861895202537 33.0 31.0 34.0 30.0 34.0 3 31.730375429795075 33.0 31.0 34.0 28.0 34.0 4 35.252185088699285 37.0 35.0 37.0 33.0 37.0 5 35.288388930289194 37.0 35.0 37.0 33.0 37.0 6 35.356911873565544 37.0 35.0 37.0 33.0 37.0 7 35.26821351562142 37.0 35.0 37.0 32.0 37.0 8 35.24984810704176 37.0 35.0 37.0 32.0 37.0 9 36.82117735021804 39.0 37.0 39.0 33.0 39.0 10-11 36.69783623831199 39.0 37.0 39.0 32.0 39.0 12-13 36.574419489912444 39.0 37.0 39.0 32.0 39.0 14-15 37.75180039653362 40.0 38.0 41.0 32.0 41.0 16-17 37.655345423186176 40.0 37.0 41.0 32.0 41.0 18-19 37.53399317907622 40.0 37.0 41.0 32.0 41.0 20-21 37.638138895880786 40.0 37.0 41.0 32.0 41.0 22-23 37.571667592490414 40.0 37.0 41.0 32.0 41.0 24-25 37.47625866564826 40.0 37.0 41.0 31.5 41.0 26-27 37.35088513296523 40.0 37.0 41.0 31.0 41.0 28-29 37.22321305684268 40.0 37.0 41.0 31.0 41.0 30-31 37.045521544621195 40.0 37.0 41.0 30.5 41.0 32-33 36.8540607807422 40.0 36.0 41.0 30.0 41.0 34-35 36.521778350355675 39.0 36.0 41.0 30.0 41.0 36-37 36.25765246023194 39.0 35.5 41.0 29.5 41.0 38-39 36.013858217533034 39.0 35.0 41.0 28.5 41.0 40-41 35.87493660793886 39.0 35.0 40.5 27.5 41.0 42-43 35.67497557313243 39.0 35.0 40.0 27.0 41.0 44-45 35.650049938684845 39.0 35.0 41.0 27.0 41.0 46-47 35.488118870809146 39.0 35.0 41.0 26.0 41.0 48-49 35.272982489531785 39.0 35.0 41.0 25.5 41.0 50-51 34.16704319587744 38.0 33.5 40.0 23.5 40.5 52-53 34.278954517668566 38.0 33.5 39.5 24.0 40.5 54-55 34.77629050117237 38.0 34.0 40.0 24.0 41.0 56-57 34.65257740651143 38.0 34.0 40.0 24.0 41.0 58-59 34.41564407574065 38.0 34.0 40.0 23.5 41.0 60-61 34.067072675510765 38.0 34.0 40.0 23.0 41.0 62-63 33.66973712598684 37.0 33.0 40.0 22.0 41.0 64-65 33.26424523459093 36.5 33.0 39.5 21.5 41.0 66-67 32.82557589130168 36.0 32.0 39.0 19.5 41.0 68-69 32.361992383033126 35.5 32.0 39.0 18.5 40.0 70-71 31.88928398279456 35.0 31.0 38.0 16.5 40.0 72-73 31.44088233069515 35.0 31.0 37.0 12.5 39.5 74-75 30.965368870499162 35.0 30.5 37.0 9.0 39.0 76-77 30.528729623094673 35.0 30.0 36.0 7.0 39.0 78-79 30.11417747173241 34.0 30.0 36.0 4.0 38.5 80-81 29.645381555111104 34.0 29.0 35.5 2.0 37.0 82-83 29.157652026252062 34.0 29.0 35.0 2.0 37.0 84-85 28.734100837085194 34.0 29.0 35.0 2.0 36.0 86-87 28.281795380966138 34.0 28.0 35.0 2.0 36.0 88-89 27.78461888506849 34.0 26.5 35.0 2.0 35.5 90-91 27.300082208188826 33.0 26.0 35.0 2.0 35.0 92-93 26.7612055155123 33.0 25.0 35.0 2.0 35.0 94-95 26.17922454615004 33.0 24.5 35.0 2.0 35.0 96-97 25.48148068355551 32.0 21.5 35.0 2.0 35.0 98-99 24.55874429161464 32.0 10.0 34.0 2.0 35.0 100-101 21.35080965146456 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14619817740197405 1101 2 0.36609673244067054 1101 3 0.4054593458029423 1101 4 0.2858622492833476 1101 5 0.20453438604866392 1101 6 0.2740645202856413 1101 7 0.325843099998373 1101 8 0.3686233494929496 1101 9 0.44690699514763566 1101 10-11 0.28186393075650784 1101 12-13 0.3308271813230874 1101 14-15 0.45633003573873765 1101 16-17 0.5295694616133559 1101 18-19 0.584798138370175 1101 20-21 0.4721292340395351 1101 22-23 0.48612879141978027 1101 24-25 0.5487021417940383 1101 26-27 0.7127856505276 1101 28-29 0.5882950932548283 1101 30-31 0.44865567643468296 1101 32-33 0.38754086707710655 1101 34-35 0.35215898875839713 1101 36-37 0.2622696462780425 1101 38-39 0.43541006430263707 1101 40-41 0.3375487918662685 1101 42-43 0.3986692648976131 1101 44-45 0.4226409847079253 1101 46-47 0.4385810411709343 1101 48-49 0.5202850698919974 1101 50-51 0.4346209058999264 1101 52-53 0.46888406722286646 1101 54-55 0.5536119086727425 1101 56-57 0.5858160883669328 1101 58-59 0.5661326606583259 1101 60-61 0.5973938192476638 1101 62-63 0.4629722691988647 1101 64-65 0.3909850773778558 1101 66-67 0.42034943622277865 1101 68-69 0.30819834452656636 1101 70-71 0.1787618524781962 1101 72-73 0.2070092045909 1101 74-75 -0.042399202000776626 1101 76-77 -0.03458367984123001 1101 78-79 0.14001249055013076 1101 80-81 0.04340304772982151 1101 82-83 0.10150938967328926 1101 84-85 0.004063944380192197 1101 86-87 -0.01555660542413051 1101 88-89 -0.2801317982296787 1101 90-91 -0.1718169177808555 1101 92-93 -0.11090762201938276 1101 94-95 -0.3228571642794762 1101 96-97 -0.3837050497979426 1101 98-99 -0.46383834463653173 1101 100-101 -0.1414801317196499 1102 1 0.2859234695832882 1102 2 0.20959078790452423 1102 3 0.3989244866970161 1102 4 0.2841798260705204 1102 5 0.24360071435295083 1102 6 0.33162353178992987 1102 7 0.41210066754840824 1102 8 0.42225907248749195 1102 9 0.39838784804705085 1102 10-11 0.42048952672099205 1102 12-13 0.4079346043557166 1102 14-15 0.4832870087519936 1102 16-17 0.5534671456200755 1102 18-19 0.6088943709375769 1102 20-21 0.5932227315048664 1102 22-23 0.5192077800363606 1102 24-25 0.564701851262214 1102 26-27 0.6884015474974703 1102 28-29 0.6707096566614581 1102 30-31 0.5692228344734644 1102 32-33 0.5362298976711131 1102 34-35 0.5265299790449873 1102 36-37 0.4916110317045508 1102 38-39 0.7456966650747603 1102 40-41 0.591194162291572 1102 42-43 0.5123352386865463 1102 44-45 0.6834385943629044 1102 46-47 0.7185358424033623 1102 48-49 0.7967883641569244 1102 50-51 0.53216169817577 1102 52-53 0.6458749448562955 1102 54-55 0.7211619436308112 1102 56-57 0.5824362717900939 1102 58-59 0.5261725034384668 1102 60-61 0.7336432641045576 1102 62-63 0.5600363453270774 1102 64-65 0.5301417810392834 1102 66-67 0.5944018395142123 1102 68-69 0.5074067561990674 1102 70-71 0.46468918958215966 1102 72-73 0.39935762765180627 1102 74-75 0.43314932928878136 1102 76-77 0.5425615012393799 1102 78-79 0.5063172307098895 1102 80-81 0.4207364646002958 1102 82-83 0.4440856085346816 1102 84-85 0.43988374589816814 1102 86-87 0.2354525768230431 1102 88-89 0.18352050836567102 1102 90-91 0.15797750877641548 1102 92-93 0.21804692361157763 1102 94-95 0.10650833972393059 1102 96-97 0.18289737459031485 1102 98-99 0.034467311474703166 1102 100-101 0.04603964135503702 1103 1 0.3534089366391058 1103 2 0.3108270741109287 1103 3 0.4346032043150636 1103 4 0.4158395051824044 1103 5 0.17354500936311013 1103 6 0.43304606233900245 1103 7 0.2561452874245873 1103 8 0.33160637132296955 1103 9 0.5053247732596589 1103 10-11 0.4912387251167658 1103 12-13 0.45173002204082735 1103 14-15 0.6083675212022612 1103 16-17 0.673966245255734 1103 18-19 0.7896940093177278 1103 20-21 0.7517479095288238 1103 22-23 0.6456687887986519 1103 24-25 0.5543373480918277 1103 26-27 0.5834776673094595 1103 28-29 0.5560742835913715 1103 30-31 0.5750357921293272 1103 32-33 0.6758164572320666 1103 34-35 0.6176596769430205 1103 36-37 0.6153071975462794 1103 38-39 0.6991263386939224 1103 40-41 0.6065416091984588 1103 42-43 0.5497531902429955 1103 44-45 0.685689147678012 1103 46-47 0.65799669528365 1103 48-49 0.7129240657673108 1103 50-51 0.5950359401211358 1103 52-53 0.6321431923740377 1103 54-55 0.7333865800500661 1103 56-57 0.5946716890395791 1103 58-59 0.699378752051679 1103 60-61 0.7922830599552313 1103 62-63 0.715916919690109 1103 64-65 0.6886834053297619 1103 66-67 0.7847441627765264 1103 68-69 0.6692366987229477 1103 70-71 0.5712397650419803 1103 72-73 0.5379107126248819 1103 74-75 0.4698780227654673 1103 76-77 0.3610526932460765 1103 78-79 0.3090801814847275 1103 80-81 0.38481622338251853 1103 82-83 0.6165791122594015 1103 84-85 0.5555313619210764 1103 86-87 0.5971240741450536 1103 88-89 0.45739242924230084 1103 90-91 0.40172283960434285 1103 92-93 0.46203812337386907 1103 94-95 0.5453042463967037 1103 96-97 0.3885075536302054 1103 98-99 0.3823350557696905 1103 100-101 0.4987759315767448 1104 1 0.3061913311843192 1104 2 0.3403866697584057 1104 3 0.3253079634630147 1104 4 0.40644415591121685 1104 5 0.3170971407654193 1104 6 0.2633371752783802 1104 7 0.2907106295144857 1104 8 0.3315867337117595 1104 9 0.39172998050318597 1104 10-11 0.39710502206109055 1104 12-13 0.4415151551106007 1104 14-15 0.49098266948195857 1104 16-17 0.5593238781619547 1104 18-19 0.620769270516945 1104 20-21 0.5556644086700331 1104 22-23 0.5590457668133126 1104 24-25 0.5805388037270447 1104 26-27 0.5719530476579777 1104 28-29 0.5268128324500196 1104 30-31 0.6545107236336705 1104 32-33 0.607688159184768 1104 34-35 0.710736637826308 1104 36-37 0.7060077904827935 1104 38-39 0.7202698617486405 1104 40-41 0.6773809282962944 1104 42-43 0.5884293027814778 1104 44-45 0.73901367048677 1104 46-47 0.7138174188656095 1104 48-49 0.630811238902794 1104 50-51 0.49074047848158386 1104 52-53 0.6488982517984283 1104 54-55 0.645951472319318 1104 56-57 0.7254278553308708 1104 58-59 0.5825189623173301 1104 60-61 0.55412348168867 1104 62-63 0.6780901067127516 1104 64-65 0.6901385414710361 1104 66-67 0.6015270403418853 1104 68-69 0.6105722103483586 1104 70-71 0.5045024825380153 1104 72-73 0.5843090716583497 1104 74-75 0.398762041189233 1104 76-77 0.5333511411044753 1104 78-79 0.2630620977401925 1104 80-81 0.48413251987659933 1104 82-83 0.7619627492686831 1104 84-85 0.692990788826144 1104 86-87 0.5499883824828906 1104 88-89 0.5469504061430435 1104 90-91 0.5696390080525155 1104 92-93 0.5044699561803334 1104 94-95 0.5177204005777725 1104 96-97 0.3619818604198173 1104 98-99 0.5176778519724046 1104 100-101 0.5288866822792251 1105 1 0.052024209591714055 1105 2 0.12293662062640465 1105 3 0.1374053540078357 1105 4 0.08649929293490999 1105 5 0.08346082675733868 1105 6 0.08334117862663248 1105 7 0.20120395120171963 1105 8 0.15308744339622393 1105 9 0.13258596914823073 1105 10-11 0.2518972118925902 1105 12-13 0.19798875120915227 1105 14-15 0.23367200965737567 1105 16-17 0.24015466391002604 1105 18-19 0.21703387363955073 1105 20-21 0.2604925376506344 1105 22-23 0.22046713741417534 1105 24-25 0.2163819634606412 1105 26-27 0.2053168610291678 1105 28-29 0.28308626113583557 1105 30-31 0.22009057223431228 1105 32-33 0.3302788714168159 1105 34-35 0.2168258215072072 1105 36-37 0.2202242260470868 1105 38-39 0.4740298246266903 1105 40-41 0.49049507831267647 1105 42-43 0.33997605097086847 1105 44-45 0.531772159404774 1105 46-47 0.5184085314324847 1105 48-49 0.4627752451625753 1105 50-51 0.4062925766000163 1105 52-53 0.44948146685101875 1105 54-55 0.4939948358597377 1105 56-57 0.3911528253057597 1105 58-59 0.2853909097642813 1105 60-61 0.5204589622724285 1105 62-63 0.42422778549864404 1105 64-65 0.5146645086435768 1105 66-67 0.46569525375674203 1105 68-69 0.5959076727350734 1105 70-71 0.4828366347380353 1105 72-73 0.44309551122598734 1105 74-75 0.4197705210087186 1105 76-77 0.3614050589266533 1105 78-79 0.36226097316327 1105 80-81 0.5245731611402 1105 82-83 0.4327169254887977 1105 84-85 0.41121421205284747 1105 86-87 0.4405491241941384 1105 88-89 0.4543798016374545 1105 90-91 0.48940144572766187 1105 92-93 0.4484572000570317 1105 94-95 0.4603565144184394 1105 96-97 0.3150903693545608 1105 98-99 0.41167100988629457 1105 100-101 0.540000522945963 1106 1 0.32315343637803906 1106 2 0.3975639973882643 1106 3 0.38130911018754077 1106 4 0.3319808066002139 1106 5 0.37955576958741233 1106 6 0.28739494961634904 1106 7 0.344300397943762 1106 8 0.3578577818009663 1106 9 0.3617688981927145 1106 10-11 0.40817948095045864 1106 12-13 0.4433446661325746 1106 14-15 0.5077429880838409 1106 16-17 0.5830213304130538 1106 18-19 0.5277953333996805 1106 20-21 0.5112628015961818 1106 22-23 0.6411801700633006 1106 24-25 0.6338835344677065 1106 26-27 0.5986103996080274 1106 28-29 0.5182842243906336 1106 30-31 0.6028972295404031 1106 32-33 0.5892910925426023 1106 34-35 0.627967677717507 1106 36-37 0.5853211539100371 1106 38-39 0.6716947312745063 1106 40-41 0.6863330755732306 1106 42-43 0.6756041183212531 1106 44-45 0.737459327713637 1106 46-47 0.6782670917026934 1106 48-49 0.7127799500014405 1106 50-51 0.6896644077887615 1106 52-53 0.6100312293180181 1106 54-55 0.8457941503442612 1106 56-57 0.7751014288277602 1106 58-59 0.7663055263485532 1106 60-61 0.9602079775292012 1106 62-63 0.9186378232519061 1106 64-65 0.8177907308788903 1106 66-67 0.7304488102058073 1106 68-69 0.7264930324220487 1106 70-71 0.7697635853642311 1106 72-73 0.6612476387132098 1106 74-75 0.785763209280006 1106 76-77 0.7213400626005217 1106 78-79 0.7380136451026296 1106 80-81 0.7287379049596794 1106 82-83 0.7234320896344855 1106 84-85 0.8511647171479808 1106 86-87 0.8596521814393476 1106 88-89 0.8552051735651993 1106 90-91 0.9244622936132671 1106 92-93 1.0088396134511761 1106 94-95 1.0824445259980706 1106 96-97 1.109467125486809 1106 98-99 1.207787840445004 1106 100-101 1.2880320592763184 1107 1 0.0914519796396398 1107 2 0.1521987141649852 1107 3 0.16917136768599406 1107 4 0.15894733728217858 1107 5 0.1552131843624096 1107 6 6.305852680554835E-5 1107 7 -0.013933492940893188 1107 8 0.07655177932826263 1107 9 0.16036558736712436 1107 10-11 0.18558887911139266 1107 12-13 0.26937486103196306 1107 14-15 0.2593029128722506 1107 16-17 0.2013162951298071 1107 18-19 0.2126237166131162 1107 20-21 0.15617587503293606 1107 22-23 0.21817486800304664 1107 24-25 0.2639348209295207 1107 26-27 0.2573613150439016 1107 28-29 0.2635214518423439 1107 30-31 0.26284816037303926 1107 32-33 0.3563054694718346 1107 34-35 0.4329023507297265 1107 36-37 0.35093879571514464 1107 38-39 0.5418275343817598 1107 40-41 0.223016869135499 1107 42-43 0.2973459133794947 1107 44-45 0.2627981317098147 1107 46-47 0.4608745016762299 1107 48-49 0.5980080977726914 1107 50-51 0.47205429836948554 1107 52-53 0.5622443738645657 1107 54-55 0.5883712760049136 1107 56-57 0.4485699870510871 1107 58-59 0.5658460692569136 1107 60-61 0.5401075116367409 1107 62-63 0.4681287476812628 1107 64-65 0.6118250760883868 1107 66-67 0.45674886948735605 1107 68-69 0.48127349721588786 1107 70-71 0.5519033286432347 1107 72-73 0.5592650747469712 1107 74-75 0.5139035628161785 1107 76-77 0.566765619745869 1107 78-79 0.5976483308051534 1107 80-81 0.6645005189293158 1107 82-83 0.6471628238817217 1107 84-85 0.5450096801506383 1107 86-87 0.8133714222425361 1107 88-89 0.707945196191222 1107 90-91 0.8476078806459846 1107 92-93 0.746940400510514 1107 94-95 0.697093835619583 1107 96-97 0.8829248879319742 1107 98-99 0.8545639624691717 1107 100-101 0.954634422398982 1108 1 0.2862975767840865 1108 2 0.21966877780362992 1108 3 0.30747447227262015 1108 4 0.2548834148274537 1108 5 0.29446533280995624 1108 6 0.25948137541639227 1108 7 0.38787259085752623 1108 8 0.44355886051158677 1108 9 0.4140958786453126 1108 10-11 0.4710692565457961 1108 12-13 0.48734447478700815 1108 14-15 0.49408398251922137 1108 16-17 0.5355997638960304 1108 18-19 0.5743853266505852 1108 20-21 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2316 48-49 -2.344858885845646 2316 50-51 -2.098707194550556 2316 52-53 -2.2595594487351818 2316 54-55 -2.2328522903635175 2316 56-57 -1.8993537766638227 2316 58-59 -1.6266927525401016 2316 60-61 -1.8480259804445396 2316 62-63 -2.0222878304462384 2316 64-65 -2.106431168592568 2316 66-67 -2.1411153390104083 2316 68-69 -2.402873073017915 2316 70-71 -2.195121858369223 2316 72-73 -2.2286841734060303 2316 74-75 -2.087888362757983 2316 76-77 -1.9925835763432183 2316 78-79 -2.005787599119156 2316 80-81 -1.960644637893651 2316 82-83 -1.7516837632806883 2316 84-85 -1.848601325208218 2316 86-87 -1.8117011304008521 2316 88-89 -1.9682636633987265 2316 90-91 -2.1411237115558386 2316 92-93 -2.1589845375697863 2316 94-95 -2.3065494429361415 2316 96-97 -2.3922412008285114 2316 98-99 -2.4474898328988566 2316 100-101 -1.966418282958081 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11712.0 3 3718.0 4 3999.0 5 4391.0 6 4942.0 7 5986.0 8 7364.0 9 8944.0 10 10801.0 11 13179.0 12 16315.0 13 20796.0 14 18287.0 15 17979.0 16 20819.0 17 25395.0 18 23938.0 19 20811.0 20 22097.0 21 23973.0 22 26816.0 23 29732.0 24 33900.0 25 38691.0 26 44818.0 27 51590.0 28 60209.0 29 71066.0 30 83720.0 31 101010.0 32 124987.0 33 158466.0 34 210050.0 35 294503.0 36 440459.0 37 619381.0 38 484656.0 39 66449.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.826444492264905 18.4651270690468 13.736747962827748 23.971680475860552 2 15.446362169955593 19.97437861629015 39.677805980536064 24.901453233218195 3 20.27841379291085 23.141459315734945 29.076222625534598 27.503904265819607 4 13.104402091833775 15.672555340720473 36.59914721747196 34.62389534997379 5 14.26045711097201 36.71299926977194 34.25942678493899 14.767116834317056 6 33.318228300627986 34.99814366281845 16.921761823720235 14.761866212833322 7 29.09665230684101 31.690167711887042 20.639253657134013 18.573926324137936 8 26.025690454618626 34.972374371083866 19.502766595150927 19.49916857914658 9 25.77643489163132 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27.713696719387936 23.162212168405922 34-35 23.407107309953474 26.185156754475752 27.5740415402898 22.833694395280972 36-37 23.19695696720992 25.91104228639286 27.924165295208507 22.967835451188705 38-39 22.863237182646444 26.42724527433396 27.809110669601 22.900406873418593 40-41 23.13274894553 26.453169575216577 27.545611704694846 22.86846977455858 42-43 22.77893515219954 26.36972887848783 27.258340142868644 23.592995826443982 44-45 23.072072034186707 26.137748934780387 27.364427762480442 23.425751268552467 46-47 23.493775315813014 26.55852274638648 27.13255549699668 22.815146440803826 48-49 23.263934591957554 26.453882005887756 27.222477596907424 23.059705805247265 50-51 22.69778185876513 26.68739870545474 27.628564730767764 22.98625470501237 52-53 23.16958685945178 26.89531121715708 26.81172953490984 23.123372388481304 54-55 22.989488039340927 26.458903861030862 27.0588953571408 23.492712742487413 56-57 23.144161989638416 26.54466504235213 27.054569213815466 23.25660375419399 58-59 23.52249828703915 26.395259352626127 26.97719119258662 23.1050511677481 60-61 23.11718526899132 26.546224457321422 27.52903350807258 22.807556765614677 62-63 22.794219790008754 26.57638268117274 27.544796783165815 23.084600745652693 64-65 22.724550963497293 26.988516790832623 27.142061369853792 23.144870875816284 66-67 22.97666490160605 26.59080177528021 26.792732187648532 23.63980113546521 68-69 22.69954010978969 26.486619215673507 27.279730008284165 23.53411066625264 70-71 23.183525833470046 26.312652130105818 27.049061944520137 23.454760091903996 72-73 23.027256544083386 26.0809777790379 27.419619421294417 23.472146255584295 74-75 22.577199295851443 27.080135844588803 26.917122338022466 23.425542521537288 76-77 22.88833871805766 26.374054765948273 26.92912473413832 23.808481781855743 78-79 22.87608466308778 26.45223978791406 27.096812871529952 23.574862677468207 80-81 23.152218633274188 26.33222176646631 26.955839120862418 23.559720479397082 82-83 23.010398675597767 26.162908950461066 27.407915577144404 23.418776796796767 84-85 22.77312471323926 26.442696599114946 27.15649528458658 23.62768340305922 86-87 22.70796575122401 26.456816574496322 27.203285021837274 23.63193265244239 88-89 22.74426657338222 26.389695765411638 26.817933041414495 24.048104619791648 90-91 22.793875283739183 26.05071508816399 26.979569532689435 24.1758400954074 92-93 22.98762011421044 26.241996085806914 27.02259143385869 23.747792366123956 94-95 22.896908046849088 25.994999394748852 27.18905007974203 23.919042478660028 96-97 22.486425690048517 25.853477679402488 27.56062776364071 24.099468866908282 98-99 22.62375747137324 26.217852531667656 26.962824465725156 24.195565531233953 100-101 22.87739088964473 25.08440907727383 27.556125533796493 24.48207449928495 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2042.0 1 1173.5 2 325.5 3 360.0 4 335.0 5 380.5 6 631.5 7 1155.0 8 1807.5 9 2162.5 10 2113.5 11 2114.5 12 2338.5 13 2614.5 14 2912.5 15 3198.0 16 3537.0 17 3767.5 18 3865.0 19 4195.5 20 4673.5 21 5223.0 22 6140.0 23 7645.0 24 9419.0 25 11525.0 26 14712.0 27 18734.5 28 22339.5 29 26070.5 30 30573.5 31 35980.5 32 41305.0 33 45850.0 34 50799.5 35 56249.0 36 63057.0 37 69158.5 38 74812.0 39 79887.0 40 84467.5 41 89078.5 42 91842.0 43 95835.5 44 101599.0 45 106504.5 46 112388.0 47 126402.5 48 148442.0 49 149428.0 50 131925.5 51 124147.0 52 123478.5 53 124175.0 54 122291.5 55 115052.0 56 106323.5 57 97404.5 58 88937.5 59 80677.0 60 68520.0 61 55578.5 62 43961.5 63 33215.5 64 24735.5 65 18214.0 66 12934.5 67 9646.5 68 7368.0 69 5551.5 70 4084.5 71 2686.0 72 1683.5 73 1069.0 74 702.5 75 450.5 76 258.5 77 170.0 78 119.5 79 84.5 80 65.0 81 56.0 82 43.0 83 29.0 84 22.5 85 23.0 86 18.5 87 11.0 88 15.5 89 15.0 90 11.5 91 13.5 92 9.0 93 6.0 94 7.0 95 6.0 96 2.0 97 1.5 98 2.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3169293071573202 2 0.08887287985329001 3 0.09916440273828905 4 0.10319421591615013 5 0.1139197186818419 6 0.0578123198208531 7 0.059362247966184285 8 0.06066418760826248 9 0.06367104821020497 10-11 0.061640642339821125 12-13 0.05971873143961046 14-15 0.057796820539399794 16-17 0.059594737187983964 18-19 0.06739087575899981 20-21 0.06844482689782502 22-23 0.08388211122532359 24-25 0.0921432282399388 26-27 0.08710596176761246 28-29 0.08509105517868192 30-31 0.07958881026275622 32-33 0.0714361882183142 34-35 0.31651082655808077 36-37 0.4919161947652107 38-39 0.4651799342582478 40-41 0.4460693202263143 42-43 0.4768973910369515 44-45 0.4867549340412579 46-47 0.48084970780754605 48-49 0.49831739800542846 50-51 0.4943805805162873 52-53 0.4935746178807151 54-55 0.48545299439917966 56-57 0.4637540003645431 58-59 0.44673578932880675 60-61 0.40525971215974427 62-63 0.47570394636504654 64-65 0.5002858067499991 66-67 0.5094458820889064 68-69 0.521411327370863 70-71 0.5188384466496133 72-73 0.5082679366984546 74-75 0.5085624230460676 76-77 0.48481752385959387 78-79 0.4647769529404617 80-81 0.4703411949822006 82-83 0.478958795470242 84-85 0.4813146862511454 86-87 0.48064821714865297 88-89 0.48677043332271114 90-91 0.500378802438719 92-93 0.5069659970563765 94-95 0.512855724008635 96-97 0.5055400631626717 98-99 0.5075084719072424 100-101 0.4865844419452714 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3225956.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.67792126086083 #Duplication Level Percentage of deduplicated Percentage of total 1 74.31028550222285 39.14511368558183 2 13.391196949699182 14.108408370098665 3 4.999560953866611 7.900994347999789 4 2.34648806375236 4.944324538475865 5 1.3406192349664463 3.5310517250178965 6 0.814552584328862 2.574536214006389 7 0.540429088973116 1.992807669720319 8 0.3800569973063412 1.6016490062994109 9 0.2876702196480256 1.3638482261741907 >10 1.4646774550479207 13.543215153379895 >50 0.08721731014699273 3.145853952101893 >100 0.034412092093496445 3.027865793037552 >500 0.001298709477829095 0.4273033497528765 >1k 0.0012396772256784475 1.4695180156407428 >5k 2.361289953673233E-4 0.7861311883231331 >10k+ 5.903224884183083E-5 0.4373787643894331 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14065 0.43599478728166163 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7449 0.23090829509143954 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6228 0.19305904978245209 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5933 0.1839144737249981 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5670 0.17576185168055608 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4810 0.14910308758085974 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4114 0.1275280877978497 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3514 0.1089289500538755 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.099856290662365E-5 5 0.0 0.0 0.0 0.0 3.099856290662365E-5 6 0.0 0.0 0.0 0.0 3.099856290662365E-5 7 3.099856290662365E-5 0.0 0.0 0.0 3.099856290662365E-5 8 3.099856290662365E-5 0.0 0.0 0.0 3.099856290662365E-5 9 3.099856290662365E-5 0.0 0.0 0.0 3.099856290662365E-5 10-11 3.099856290662365E-5 0.0 0.0 0.0 3.099856290662365E-5 12-13 3.099856290662365E-5 0.0 0.0 0.0 1.8599137743974188E-4 14-15 3.099856290662365E-5 0.0 0.0 0.0 2.9448634761292466E-4 16-17 3.099856290662365E-5 0.0 0.0 0.0 3.4098419197286015E-4 18-19 3.099856290662365E-5 0.0 0.0 0.0 4.0298131778610744E-4 20-21 3.099856290662365E-5 0.0 0.0 0.0 4.0298131778610744E-4 22-23 3.099856290662365E-5 0.0 0.0 4.649784435993547E-5 4.4947916214604293E-4 24-25 3.099856290662365E-5 0.0 0.0 1.239942516264946E-4 4.959770065059784E-4 26-27 3.099856290662365E-5 0.0 0.0 4.8047772505266655E-4 6.044719766791612E-4 28-29 3.099856290662365E-5 0.0 0.0 0.002231896529276903 6.509698210390967E-4 30-31 3.099856290662365E-5 0.0 0.0 0.007176167312883374 6.509698210390967E-4 32-33 3.099856290662365E-5 0.0 0.0 0.016692726125216835 7.439655097589675E-4 34-35 3.099856290662365E-5 0.0 0.0 0.03191302051236905 7.439655097589675E-4 36-37 3.099856290662365E-5 0.0 0.0 0.05726984496998719 7.439655097589675E-4 38-39 3.099856290662365E-5 0.0 0.0 0.0958165579443737 7.749640726655913E-4 40-41 3.099856290662365E-5 0.0 0.0 0.14124495188403066 7.749640726655913E-4 42-43 3.099856290662365E-5 0.0 0.0 0.1899436942103364 8.214619170255267E-4 44-45 3.099856290662365E-5 0.0 0.0 0.24750802552793652 9.144576057453977E-4 46-47 3.099856290662365E-5 0.0 0.0 0.3201066598552491 0.0010229525759185803 48-49 3.099856290662365E-5 0.0 0.0 0.4009819104786302 0.0011159482646384514 50-51 3.099856290662365E-5 0.0 0.0 0.4888163384745483 0.001146946827545075 52-53 3.099856290662365E-5 0.0 0.0 0.5771932413213323 0.0011934446719050105 54-55 3.099856290662365E-5 0.0 0.0 0.6798604816680698 0.0012554417977182577 56-57 3.099856290662365E-5 0.0 0.0 0.805327165032629 0.0012864403606248813 58-59 3.099856290662365E-5 0.0 0.0 0.9402949079280685 0.0013174389235315052 60-61 3.099856290662365E-5 0.0 0.0 1.0879100644894102 0.0013949353307980642 62-63 3.099856290662365E-5 0.0 0.0 1.2440498258500736 0.0014569324566113115 64-65 3.099856290662365E-5 0.0 0.0 1.4118915447079874 0.001487931019517935 66-67 3.099856290662365E-5 0.0 0.0 1.6065470204801304 0.001487931019517935 68-69 3.099856290662365E-5 0.0 0.0 1.8312556029902454 0.001487931019517935 70-71 3.099856290662365E-5 0.0 0.0 2.076872716180878 0.0015499281453311823 72-73 3.099856290662365E-5 0.0 0.0 2.3257446784767057 0.0017049209598643008 74-75 3.099856290662365E-5 1.5499281453311824E-5 0.0 2.5918363424671633 0.0018289152114907953 76-77 3.099856290662365E-5 3.099856290662365E-5 0.0 2.8806344537867226 0.001859913774397419 78-79 3.099856290662365E-5 3.099856290662365E-5 0.0 3.2247804991760582 0.0018909123373040426 80-81 6.19971258132473E-5 3.099856290662365E-5 0.0 3.598654166392846 0.0018909123373040426 82-83 6.19971258132473E-5 3.099856290662365E-5 0.0 3.9751472121752434 0.0018909123373040426 84-85 6.19971258132473E-5 3.099856290662365E-5 0.0 4.352833702629546 0.0019064116187573544 86-87 6.19971258132473E-5 3.099856290662365E-5 0.0 4.758635889640156 0.0019839080260239134 88-89 6.19971258132473E-5 3.099856290662365E-5 0.0 5.2098509713089705 0.0020614044332904723 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8835 0.0 21.755665 1 TACATGG 8590 0.0 21.61524 2 TAACCCG 165 6.466196E-5 20.094687 5 ACATGGG 8840 0.0 20.03948 3 AGAGTAC 10810 0.0 19.094826 10-11 GAGTACT 7020 0.0 18.715046 12-13 GAGTACA 7170 0.0 17.871809 1 CATGGGG 6235 0.0 17.700542 4 GTACTTT 7485 0.0 17.521305 14-15 AGTACTT 7540 0.0 17.424355 12-13 TTTGCGT 560 0.0 16.916445 3 GTATAGG 900 0.0 16.874517 1 GTGTAGC 1920 0.0 16.808603 1 AGTACAT 6870 0.0 16.54709 2 GTATTAG 1060 0.0 16.118347 1 GTCTTAG 1440 0.0 15.160699 1 ACTTTTT 9260 0.0 14.624263 16-17 TACTTTT 8875 0.0 14.430367 14-15 TATACAG 1915 0.0 14.098509 5 TATACTG 1535 0.0 13.885812 5 >>END_MODULE