FastQCFastQC Report
Fri 27 May 2016
SRR938983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3225956
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT157100.4869874232630575No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67970.21069723207632093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63180.19584892044404822No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT54040.1675162339473942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53630.16624529286822262No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC51500.1596425989691118No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41670.12917101163190076No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35170.10902194574259538No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34920.10824698166992978No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC34740.10768900753761056No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG33860.10496113400182767No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32520.10080732657234011No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86650.022.7657451
TACATGG85100.022.3609872
ACATGGG85550.020.6902123
GTAAGCG5100.020.0055894-95
GAGTACT75150.019.48195812-13
GTACTTT76700.018.96465514-15
CATGGGG62200.018.5392444
AGAGTAC113900.018.499610-11
GAGTACA68850.017.8552461
AGTACTT79100.017.84912512-13
GTGTACG3751.6552804E-1017.7888091
TACACTG23150.016.3989165
GTATAGC12850.016.3154721
AGTACAT67500.016.2399372
CCTATAC8000.016.0158483
GTACTAG4208.258212E-1015.8828641
TTTGCGT6400.015.5709633
GTGTAGG12700.015.0074321
TACTTTT91800.014.78540214-15
GTACCGA2901.8832421E-514.7272176
Produced by FastQC (version 0.11.5)