##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938983_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3225956 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.164993570898055 33.0 31.0 34.0 30.0 34.0 2 32.404034338968046 34.0 31.0 34.0 31.0 34.0 3 32.50535097192894 34.0 31.0 34.0 31.0 34.0 4 36.05164794560124 37.0 35.0 37.0 35.0 37.0 5 35.97011180561669 37.0 35.0 37.0 35.0 37.0 6 36.00792416263582 37.0 35.0 37.0 35.0 37.0 7 35.94128221215664 37.0 35.0 37.0 35.0 37.0 8 35.906453466817275 37.0 35.0 37.0 35.0 37.0 9 37.58754025163393 39.0 37.0 39.0 35.0 39.0 10-11 37.492042513909055 39.0 37.0 39.0 34.5 39.0 12-13 37.398928720664514 39.0 37.0 39.0 34.0 39.0 14-15 38.72008065206097 40.0 38.0 41.0 34.0 41.0 16-17 38.815278943668176 40.0 38.0 41.0 35.0 41.0 18-19 38.82913003153173 40.0 38.0 41.0 34.5 41.0 20-21 38.78921612694036 40.0 38.0 41.0 34.0 41.0 22-23 38.71341673600012 40.0 38.0 41.0 34.0 41.0 24-25 38.63645226407304 40.0 38.0 41.0 34.0 41.0 26-27 38.473508783132814 40.0 38.0 41.0 34.0 41.0 28-29 38.318853387956935 40.0 38.0 41.0 34.0 41.0 30-31 38.14713064902311 40.0 38.0 41.0 33.0 41.0 32-33 38.09236238807969 40.0 38.0 41.0 33.0 41.0 34-35 38.00441233544413 40.0 37.5 41.0 33.0 41.0 36-37 37.888715624143664 40.0 37.0 41.0 33.0 41.0 38-39 37.75535949653374 40.0 37.0 41.0 33.0 41.0 40-41 37.60319747076525 40.0 37.0 41.0 32.0 41.0 42-43 37.430732471242635 40.0 37.0 41.0 32.0 41.0 44-45 37.205900049473705 40.0 36.5 41.0 31.0 41.0 46-47 36.94816203320814 40.0 36.0 41.0 31.0 41.0 48-49 36.646513777621266 39.0 36.0 41.0 30.0 41.0 50-51 36.387389970600964 39.0 35.0 40.5 30.0 41.0 52-53 36.13119134296934 39.0 35.0 40.0 29.5 41.0 54-55 35.82040083621723 38.5 35.0 40.0 28.5 41.0 56-57 35.65383238333071 38.0 35.0 40.0 28.0 41.0 58-59 35.34750148483116 38.0 34.0 40.0 28.0 41.0 60-61 34.94624508207799 37.5 34.0 40.0 27.5 41.0 62-63 34.50801483343233 37.0 34.0 40.0 26.0 41.0 64-65 34.0772547734687 36.5 33.0 39.0 26.0 41.0 66-67 33.59386163357467 36.0 33.0 39.0 25.5 40.0 68-69 33.07369133366977 35.0 32.0 38.5 24.5 40.0 70-71 32.51658919092511 35.0 31.5 37.5 23.0 40.0 72-73 31.9838108455292 35.0 31.0 37.0 22.0 39.0 74-75 31.445166022103216 34.5 31.0 36.5 20.5 39.0 76-77 29.566420310754395 32.5 28.5 35.0 18.0 37.0 78-79 30.53724477333231 34.0 30.0 35.0 19.0 37.0 80-81 30.51184176101596 34.0 30.0 35.0 18.0 37.0 82-83 30.078611580567127 34.0 30.0 35.0 16.5 36.5 84-85 29.60472693985907 34.0 29.0 35.0 9.0 36.0 86-87 28.894121308536135 33.5 29.0 35.0 4.5 36.0 88-89 28.171949183435856 33.0 27.5 35.0 2.0 35.0 90-91 27.548221674443173 33.0 26.0 34.5 2.0 35.0 92-93 27.092936946443164 32.0 25.5 34.0 2.0 35.0 94-95 26.476904985684865 32.0 25.0 34.0 2.0 35.0 96-97 25.548782128460523 32.0 23.5 34.0 2.0 35.0 98-99 24.237812760000445 31.5 10.0 34.0 2.0 35.0 100-101 18.601026641404903 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07900554378669256 1101 2 0.14777270406727183 1101 3 0.19504213509646462 1101 4 0.08319245419005483 1101 5 0.07146926893070571 1101 6 0.11189735773115927 1101 7 0.12919751320392692 1101 8 0.1606684097250053 1101 9 0.15633812279887138 1101 10-11 0.21437885560703052 1101 12-13 0.27406277456645967 1101 14-15 0.2465137599474545 1101 16-17 0.2655764353322283 1101 18-19 0.23617750059023734 1101 20-21 0.27960217415520816 1101 22-23 0.2481468845932966 1101 24-25 0.20263695016357275 1101 26-27 0.20142724798334655 1101 28-29 0.28586060046068695 1101 30-31 0.18666504421701546 1101 32-33 0.1861493864294559 1101 34-35 0.25163536662491026 1101 36-37 0.39148385052181567 1101 38-39 0.2892167078156618 1101 40-41 0.32558215275414426 1101 42-43 0.2931765454475226 1101 44-45 0.2719535967882436 1101 46-47 0.3068324996399383 1101 48-49 0.24358403896939507 1101 50-51 0.23510327364640915 1101 52-53 0.25792464100979373 1101 54-55 0.20463201873744197 1101 56-57 0.1800763543267081 1101 58-59 0.24734748706551102 1101 60-61 0.20010594882989352 1101 62-63 0.19201924867713416 1101 64-65 0.140599646879636 1101 66-67 0.10702856860908838 1101 68-69 0.1297658234728516 1101 70-71 0.19838911087166622 1101 72-73 0.2903697619896235 1101 74-75 0.22615225216434354 1101 76-77 -0.07391672152129303 1101 78-79 0.3369727458969294 1101 80-81 0.3344824502396584 1101 82-83 0.33445403079123537 1101 84-85 0.39775276908192225 1101 86-87 0.439514026204467 1101 88-89 0.48277561836152927 1101 90-91 0.5456830730021629 1101 92-93 0.3976771321291537 1101 94-95 0.2812086790022654 1101 96-97 0.34792831213301767 1101 98-99 0.6680390787158608 1101 100-101 0.9127898132293986 1102 1 0.10617825331198105 1102 2 0.10590961885218775 1102 3 0.12913255148290403 1102 4 0.08404752704755936 1102 5 0.07118539050829042 1102 6 0.11984395753854216 1102 7 0.1340713508687159 1102 8 0.1286916123504369 1102 9 0.1745304049651537 1102 10-11 0.2726254960556034 1102 12-13 0.23549312261138766 1102 14-15 0.3106466638407497 1102 16-17 0.32514377578506526 1102 18-19 0.2840159493720833 1102 20-21 0.2307985387371545 1102 22-23 0.270141917829811 1102 24-25 0.28953507384918 1102 26-27 0.2998135765759429 1102 28-29 0.35074284358814367 1102 30-31 0.29171669401083733 1102 32-33 0.3769612066390451 1102 34-35 0.2864939181019892 1102 36-37 0.448764805329148 1102 38-39 0.435070059105783 1102 40-41 0.3998160275449152 1102 42-43 0.5178407566397709 1102 44-45 0.4249604070981263 1102 46-47 0.4581997457253877 1102 48-49 0.5364012495423154 1102 50-51 0.5563851177916135 1102 52-53 0.5643115728440335 1102 54-55 0.4020511092286796 1102 56-57 0.4676860037534851 1102 58-59 0.37547113386087716 1102 60-61 0.5161104820134526 1102 62-63 0.6241056053803575 1102 64-65 0.4506833743807448 1102 66-67 0.313231977478857 1102 68-69 0.3070379181684828 1102 70-71 0.3359219412448837 1102 72-73 0.42759742305874227 1102 74-75 0.39381766817652775 1102 76-77 0.23595720208325588 1102 78-79 0.44526338195647597 1102 80-81 0.4862978028504834 1102 82-83 0.4486962999556603 1102 84-85 0.552315643439659 1102 86-87 0.7278102511204558 1102 88-89 0.4722778830127332 1102 90-91 0.5240254226172922 1102 92-93 0.5601289989347293 1102 94-95 0.7400867271606835 1102 96-97 0.7647385166751803 1102 98-99 1.1288084668636031 1102 100-101 1.101155566180875 1103 1 0.21505242771214483 1103 2 0.15122595866351674 1103 3 0.12211049071400737 1103 4 0.12074195288529666 1103 5 0.12072990593142663 1103 6 0.1068926601591329 1103 7 0.12953295136657061 1103 8 0.11796446625291424 1103 9 0.21187487086247359 1103 10-11 0.16885747488029068 1103 12-13 0.13802440654556847 1103 14-15 0.22735385946189268 1103 16-17 0.20397851984871096 1103 18-19 0.31875860490398367 1103 20-21 0.2947821611159185 1103 22-23 0.2596873177210526 1103 24-25 0.23401335812184954 1103 26-27 0.24800288663470837 1103 28-29 0.20530102811392936 1103 30-31 0.20818401615931492 1103 32-33 0.2673191731060456 1103 34-35 0.2778654586243263 1103 36-37 0.17798912789422872 1103 38-39 0.17013643656751753 1103 40-41 0.30927784519949597 1103 42-43 0.3739800929300543 1103 44-45 0.34732797862481135 1103 46-47 0.4295221872666062 1103 48-49 0.3564554969199065 1103 50-51 0.36513621471358704 1103 52-53 0.33279929408345055 1103 54-55 0.4357990681589925 1103 56-57 0.5686119560610692 1103 58-59 0.4247805611798725 1103 60-61 0.41341647354786204 1103 62-63 0.5080238959279129 1103 64-65 0.5044595856867815 1103 66-67 0.36698805723455763 1103 68-69 0.23514820694182248 1103 70-71 0.19488365488644632 1103 72-73 0.17859060704384433 1103 74-75 0.34159142123325026 1103 76-77 0.1695564735631514 1103 78-79 0.4107040005385123 1103 80-81 0.30448047609814566 1103 82-83 0.30190193032125734 1103 84-85 0.5136299542547356 1103 86-87 0.6464831881233337 1103 88-89 0.6093363928885793 1103 90-91 0.5903777474098248 1103 92-93 0.7801593634731034 1103 94-95 0.8295765837745428 1103 96-97 0.6612020083286758 1103 98-99 0.8878353495238365 1103 100-101 1.1097934313828723 1104 1 0.007874435984462025 1104 2 0.048262274248386916 1104 3 0.042235103834073584 1104 4 0.06426288109984313 1104 5 0.044250632345111285 1104 6 0.09305888526886719 1104 7 0.08995654657486796 1104 8 0.13228722851185637 1104 9 0.08253906803624034 1104 10-11 0.10854190823267373 1104 12-13 0.09723410444424019 1104 14-15 0.10038786062951033 1104 16-17 0.15471664991630973 1104 18-19 0.18760664852595 1104 20-21 0.2692031960570844 1104 22-23 0.18356222635462416 1104 24-25 0.22500663826149037 1104 26-27 0.14777083733714846 1104 28-29 0.22026743749172084 1104 30-31 0.10080707698589464 1104 32-33 0.2094050910184393 1104 34-35 0.21054110919072144 1104 36-37 0.2523672313284564 1104 38-39 0.23309440112151947 1104 40-41 0.24627604862930497 1104 42-43 0.18144031293422813 1104 44-45 0.2714377791272966 1104 46-47 0.20589639420818884 1104 48-49 0.1121561815347718 1104 50-51 0.2393617632023748 1104 52-53 0.19675122555101154 1104 54-55 0.3132521285722021 1104 56-57 0.2250234137067011 1104 58-59 0.21768931127629543 1104 60-61 0.29790728493598095 1104 62-63 0.23887269532666977 1104 64-65 0.35408642225097253 1104 66-67 0.21825013178869312 1104 68-69 0.26427376798348945 1104 70-71 0.19907406073077283 1104 72-73 0.31059913215399604 1104 74-75 0.22295361397108948 1104 76-77 0.27442430104771276 1104 78-79 0.39539192580535953 1104 80-81 0.3272299434686303 1104 82-83 0.33027865825474123 1104 84-85 0.32420935383639815 1104 86-87 0.46593222401969214 1104 88-89 0.3509113965746984 1104 90-91 0.5133122748545773 1104 92-93 0.6791680655387502 1104 94-95 0.8389294572181676 1104 96-97 0.7707480898277979 1104 98-99 0.8592822085747223 1104 100-101 1.176461168534427 1105 1 -0.09134491110582843 1105 2 -0.08450331151424706 1105 3 -0.017910654436477103 1105 4 -0.07840644233429828 1105 5 -0.08595826964564424 1105 6 -0.11745809256714068 1105 7 -0.062457290452464065 1105 8 -0.0499561022738888 1105 9 -0.030405394605246272 1105 10-11 0.009486334480087066 1105 12-13 -0.003158951566234691 1105 14-15 -0.033312597935832855 1105 16-17 -0.08677168578263661 1105 18-19 -0.06023927715735056 1105 20-21 0.008077982241893267 1105 22-23 -0.1104018162992304 1105 24-25 -0.11775276050838812 1105 26-27 -0.20541091467362094 1105 28-29 0.008565377587082423 1105 30-31 -0.04295861795896627 1105 32-33 -0.02681747694749248 1105 34-35 0.06569723977266051 1105 36-37 -0.020574683727772936 1105 38-39 -0.014055487876554196 1105 40-41 0.04401597656903533 1105 42-43 0.21485689771422756 1105 44-45 0.09014751818177302 1105 46-47 0.10564436523850418 1105 48-49 0.022137711541681426 1105 50-51 -0.02847588282885738 1105 52-53 0.03473617660097972 1105 54-55 0.09585894518245652 1105 56-57 0.08901648063541501 1105 58-59 -0.01783722000440946 1105 60-61 0.057017176845583606 1105 62-63 -0.0841997834737711 1105 64-65 -0.1452152664341071 1105 66-67 0.008347303969060249 1105 68-69 0.12161809232748766 1105 70-71 0.28924720554280015 1105 72-73 0.0836502294353636 1105 74-75 0.0923989682674069 1105 76-77 -0.10770893766102319 1105 78-79 0.10464420953356779 1105 80-81 0.08745440392646842 1105 82-83 0.16812713152329195 1105 84-85 0.15404658133576277 1105 86-87 0.17008478798227955 1105 88-89 0.40855733681562967 1105 90-91 0.16531667853765697 1105 92-93 0.28643277784358645 1105 94-95 0.20318183193156258 1105 96-97 0.45879855021284754 1105 98-99 0.6166429102524873 1105 100-101 0.6762769235295103 1106 1 -0.0035194794470925217 1106 2 0.03308165787566253 1106 3 0.03206393943364816 1106 4 0.02818659155853709 1106 5 0.0677619078126952 1106 6 0.06816191518970527 1106 7 0.09864109713788594 1106 8 0.14639932498275243 1106 9 0.05832602537240206 1106 10-11 0.1034313295952316 1106 12-13 0.1152851623121336 1106 14-15 0.023448930555851177 1106 16-17 -0.051787526770539216 1106 18-19 -0.026760359744862683 1106 20-21 0.061822277113371626 1106 22-23 0.08168489019901415 1106 24-25 0.14339238908912222 1106 26-27 0.017070482802623133 1106 28-29 0.05967414446108421 1106 30-31 0.1247709331748581 1106 32-33 0.15619778882272328 1106 34-35 0.06947563449800498 1106 36-37 0.0682688341497979 1106 38-39 0.15238769894401116 1106 40-41 0.25695616538444455 1106 42-43 0.26733190888310787 1106 44-45 0.273213718946657 1106 46-47 0.30612961822792073 1106 48-49 0.26399128304623787 1106 50-51 0.3183220856626363 1106 52-53 0.27534194301513537 1106 54-55 0.30709813018981436 1106 56-57 0.2984424616556822 1106 58-59 0.23781196184457087 1106 60-61 0.3170572007052428 1106 62-63 0.38545574458915866 1106 64-65 0.3853139215679775 1106 66-67 0.42606335426092556 1106 68-69 0.4143981693395631 1106 70-71 0.5415780440841829 1106 72-73 0.631227810182672 1106 74-75 0.6459494442334552 1106 76-77 0.3406247720594173 1106 78-79 0.5639614678644058 1106 80-81 0.5192396611706904 1106 82-83 0.49972489071081583 1106 84-85 0.6178857012117831 1106 86-87 0.8349832534684047 1106 88-89 0.8446301341737161 1106 90-91 0.8996665434474167 1106 92-93 1.0752467779712305 1106 94-95 1.2339701951876698 1106 96-97 1.0625425996144742 1106 98-99 1.0493997167224158 1106 100-101 1.375436127912618 1107 1 0.015417237645984017 1107 2 0.022809129131523775 1107 3 0.020809466001992405 1107 4 0.006630219252748759 1107 5 0.016780674824566688 1107 6 0.037745386602743736 1107 7 0.03562993909471146 1107 8 -0.05083583312777762 1107 9 -0.1071269432260138 1107 10-11 -0.055516205328657975 1107 12-13 -0.03065882273536147 1107 14-15 -0.04105579382502356 1107 16-17 -0.09524599690553259 1107 18-19 -0.00153978806380195 1107 20-21 0.036221547691660305 1107 22-23 0.02507857564111049 1107 24-25 0.0348750213183564 1107 26-27 0.09319605544497733 1107 28-29 -0.05235736437571603 1107 30-31 -0.03423308662361535 1107 32-33 0.03075290152082033 1107 34-35 0.018319684320168506 1107 36-37 0.05006972832490675 1107 38-39 0.0028319397020908355 1107 40-41 0.01276528640361363 1107 42-43 0.012274482761007732 1107 44-45 -0.047463644461679166 1107 46-47 -0.11150100016267572 1107 48-49 -0.10984446194565578 1107 50-51 -0.243009662285111 1107 52-53 -0.17589356713600068 1107 54-55 -0.20909984734093 1107 56-57 -0.09329347541486044 1107 58-59 -0.19701842615589982 1107 60-61 -0.17844931976433287 1107 62-63 -0.034437476444033166 1107 64-65 0.008290865915689949 1107 66-67 -0.05148543674223305 1107 68-69 -0.1215465093642436 1107 70-71 -0.024461044050461567 1107 72-73 0.10901426868829134 1107 74-75 0.028935037232418637 1107 76-77 -0.14338128760985924 1107 78-79 0.07103315363568896 1107 80-81 0.01778864643771172 1107 82-83 -0.10019123902376492 1107 84-85 0.1152615853479233 1107 86-87 0.16964676299645376 1107 88-89 0.41677720695410514 1107 90-91 0.4666106926869702 1107 92-93 0.5588709758308816 1107 94-95 0.4882325728931569 1107 96-97 0.6607754600385967 1107 98-99 0.7833795136815986 1107 100-101 0.9224400639109973 1108 1 0.04548443169785088 1108 2 0.059010645792810124 1108 3 0.13139911758781153 1108 4 0.07873291434395213 1108 5 -0.014793824182888216 1108 6 0.07364531752183012 1108 7 0.11897083546242726 1108 8 0.14851514936496102 1108 9 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1630.0 10 2643.0 11 3952.0 12 5845.0 13 7928.0 14 9718.0 15 12847.0 16 16463.0 17 21963.0 18 22188.0 19 18865.0 20 19270.0 21 21165.0 22 23380.0 23 26122.0 24 30218.0 25 35580.0 26 42882.0 27 51622.0 28 62406.0 29 75137.0 30 90810.0 31 110253.0 32 137410.0 33 173620.0 34 232630.0 35 332039.0 36 511772.0 37 702019.0 38 403466.0 39 18737.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.67482108909649 19.190993165956687 14.275187172997672 23.858998571949147 2 15.89125828126608 20.569437400882094 38.9346599891629 24.60464432868892 3 20.302911757010943 23.46172731432171 29.35790196766478 26.87745896100257 4 13.531151695807383 16.302795202414416 36.19206213599938 33.973990965778825 5 14.133577767334707 36.9498530048147 34.243089490371226 14.67347973747937 6 33.25104248167055 35.53011882369133 16.558316356453716 14.6605223381844 7 29.037035843018316 31.772194041084255 20.56587256614783 18.624897549749594 8 25.9832744153981 35.37001124627862 19.26365393700348 19.383060401319796 9 25.550906459976513 17.17156092643545 20.338157123035778 36.93937549055226 10-11 25.52942445588223 26.004678923085127 26.89815050174274 21.56774611928991 12-13 26.28602188002564 23.181562302771646 27.391911111000894 23.14050470620182 14-15 22.85306164328995 24.85507284563743 25.684197833121665 26.607667677950953 16-17 22.650972383418146 27.84656503883582 26.44129306411134 23.061169513634695 18-19 22.599357366302293 27.337754055518953 27.723517166373185 22.33937141180557 20-21 23.409107253787713 26.076518092621225 27.66615539703579 22.848219256555264 22-23 22.943539837493134 25.96169941561509 27.82097771947293 23.273783027418848 24-25 23.282950320137758 26.11949019747641 27.736375739897177 22.86118374248866 26-27 22.98007164387859 26.028919799278107 28.181893987394744 22.80911456944856 28-29 22.66636618726356 26.97318252325822 27.734429731837633 22.626021557640588 30-31 22.930597937479618 26.21567064150906 27.568122441843595 23.285608979167726 32-33 22.92100388226002 26.233494815180368 27.783190471289753 23.062310831269862 34-35 23.381903635487117 26.227699643318303 27.689056025843534 22.701340695351046 36-37 23.2051565671027 25.954822367840723 27.988558393908807 22.85146267114777 38-39 22.83063998726452 26.52231677209554 27.872252730431796 22.774790510208145 40-41 23.103766883301375 26.493377500380916 27.58828979068186 22.81456582563585 42-43 22.772766015753298 26.46358632531677 27.2804223323792 23.483225326550727 44-45 23.090993070161705 26.208970815941573 27.392576656783135 23.307459457113584 46-47 23.495550031208136 26.62178853391194 27.147018301592276 22.73564313328765 48-49 23.292482300107224 26.542352800037943 27.230152390039642 22.93501250981519 50-51 22.72449975030526 26.757480535124813 27.644447089669082 22.87357262490084 52-53 23.213397826876747 26.94683374478759 26.756766056325628 23.083002372010032 54-55 23.016215968227712 26.57785785051005 26.959047178572803 23.446879002689435 56-57 23.17967139043434 26.608685921320685 27.024578140557402 23.18706454768757 58-59 23.549376808101226 26.477026410193023 26.94565059870286 23.027946183002896 60-61 23.07801281032886 26.705126068980483 27.46939044378916 22.747470676901496 62-63 22.853690015652926 26.64202662531818 27.480920526566965 23.023362832461927 64-65 22.68828542187866 27.08634570427444 27.07958797042778 23.14578090341912 66-67 22.99171791626406 26.650999619018567 26.735379721310064 23.6219027434073 68-69 22.761248683497744 26.575172181832695 27.199916921829796 23.46366221283976 70-71 23.265001071001347 26.412241591864483 26.91338032513183 23.409377012002345 72-73 23.084319810933565 26.22647364068202 27.248465261150496 23.440741287233923 74-75 22.6396609253195 27.205811238590975 26.683268463674025 23.471259372415496 76-77 22.884740057513856 26.482714584433158 26.814139838683833 23.818405519369158 78-79 22.931005006299916 26.534149059987715 26.960100724292978 23.57474520941939 80-81 23.171755040144777 26.419125038864532 26.819487846832047 23.589632074158644 82-83 23.023981108235823 26.269887128032742 27.263158579968234 23.4429731837632 84-85 22.75201456191425 26.64719333021177 26.94619057117912 23.654601536694855 86-87 22.71934330229996 26.57372219449646 26.990037807526473 23.716896695677104 88-89 22.728014957358422 26.497490943908502 26.621445490898456 24.153048607834616 90-91 22.78257516975849 26.197037941293118 26.767751340043027 24.252635548905367 92-93 22.990208173949057 26.46333365985153 26.760764870940584 23.78569329525883 94-95 22.868261269720325 26.17207540291569 26.938166224054328 24.02149710330966 96-97 22.40428883716951 26.049673337144092 27.32763559081401 24.21840223487239 98-99 22.55784021852747 26.399476620263883 26.70833080178403 24.334352359424617 100-101 22.848559320483965 25.236093756151302 27.42697251625339 24.488374407111348 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 334.0 1 251.0 2 208.5 3 261.5 4 239.0 5 238.0 6 439.0 7 877.5 8 1435.5 9 1728.5 10 1631.0 11 1577.5 12 1742.5 13 1920.5 14 2098.5 15 2267.0 16 2471.0 17 2757.5 18 3016.0 19 3308.5 20 3858.5 21 4741.5 22 5840.0 23 7334.5 24 9144.5 25 11372.5 26 14733.5 27 18943.0 28 22704.0 29 26610.0 30 30904.5 31 36204.0 32 41843.0 33 46303.0 34 51212.0 35 56575.5 36 63097.5 37 69463.0 38 75536.0 39 80458.5 40 84220.0 41 89346.5 42 92794.5 43 96505.5 44 102230.5 45 107013.5 46 112772.0 47 128309.5 48 150963.0 49 150067.0 50 131907.0 51 125067.0 52 125107.0 53 125958.0 54 123609.0 55 115669.0 56 107077.5 57 98138.5 58 89848.0 59 81317.5 60 68167.0 61 55076.0 62 43513.5 63 32447.5 64 23766.0 65 17194.5 66 12067.0 67 9039.5 68 6796.5 69 5010.0 70 3649.0 71 2288.5 72 1375.5 73 889.5 74 525.0 75 300.0 76 185.5 77 96.0 78 51.0 79 28.0 80 16.5 81 12.5 82 7.0 83 5.5 84 3.5 85 1.5 86 2.5 87 3.0 88 2.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38701705788919627 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.749640726655912E-4 16-17 0.002107902277650408 18-19 3.874820363327956E-4 20-21 0.0 22-23 0.0 24-25 3.099856290662365E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012120438096489846 36-37 0.021993480382249478 38-39 0.009966037974479503 40-41 0.0053472521013925795 42-43 0.0048202765319799775 44-45 0.0015809267082378062 46-47 0.001069450420278516 48-49 0.002758872098689505 50-51 5.26975569412602E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.284662283056557E-4 60-61 0.0013484374864381288 62-63 0.0012864403606248813 64-65 6.974676653990321E-4 66-67 0.002386889343810021 68-69 0.0024333871881699566 70-71 9.299568871987094E-5 72-73 0.0 74-75 0.0 76-77 0.09009732308810163 78-79 0.0037353268302481495 80-81 2.1698994034636556E-4 82-83 0.0 84-85 2.014906588930537E-4 86-87 0.09053130296879437 88-89 0.43061653661736243 90-91 0.3827237569266289 92-93 0.0 94-95 0.0010229525759185803 96-97 0.0 98-99 0.0 100-101 4.029813177861074E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3225956.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.66976152180253 #Duplication Level Percentage of deduplicated Percentage of total 1 72.64996159640886 36.81156228658149 2 14.227043503287904 14.41761802943816 3 5.243904298837252 7.97122140795716 4 2.504592600755353 5.0762843915817975 5 1.394111390706701 3.531964585096851 6 0.9104163737986148 2.7678348327552023 7 0.5918986338288446 2.099395383483179 8 0.42280300850414226 1.7138662089284427 9 0.31854068943281233 1.4526342691696046 >10 1.5964022148355101 14.251418157363883 >50 0.09893472447931094 3.400610399095306 >100 0.03839517193251716 3.2695754407306965 >500 0.0013450500062795424 0.4209399531141545 >1k 0.0012839113668011504 1.4290211260671237 >5k 3.056931825717025E-4 0.8993765599076662 >10k+ 6.11386365143405E-5 0.4866769687293137 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15710 0.4869874232630575 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6797 0.21069723207632093 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6318 0.19584892044404822 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5404 0.1675162339473942 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5363 0.16624529286822262 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5150 0.1596425989691118 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4167 0.12917101163190076 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3517 0.10902194574259538 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3492 0.10824698166992978 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3474 0.10768900753761056 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3386 0.10496113400182767 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3252 0.10080732657234011 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.19971258132473E-5 2 0.0 0.0 0.0 0.0 6.19971258132473E-5 3 0.0 0.0 0.0 0.0 6.19971258132473E-5 4 0.0 0.0 0.0 0.0 6.19971258132473E-5 5 0.0 0.0 0.0 0.0 6.19971258132473E-5 6 0.0 0.0 0.0 0.0 9.299568871987094E-5 7 0.0 0.0 0.0 0.0 9.299568871987094E-5 8 0.0 0.0 0.0 0.0 9.299568871987094E-5 9 0.0 0.0 0.0 0.0 9.299568871987094E-5 10-11 0.0 0.0 0.0 0.0 9.299568871987094E-5 12-13 0.0 0.0 0.0 1.5499281453311824E-5 1.239942516264946E-4 14-15 0.0 0.0 0.0 3.099856290662365E-5 2.014906588930537E-4 16-17 0.0 0.0 0.0 3.099856290662365E-5 3.099856290662365E-4 18-19 0.0 0.0 0.0 3.099856290662365E-5 3.7198275487948377E-4 20-21 0.0 0.0 0.0 6.19971258132473E-5 3.874820363327956E-4 22-23 0.0 0.0 0.0 1.0849497017318277E-4 4.6497844359935474E-4 24-25 0.0 0.0 0.0 1.7049209598643007E-4 4.959770065059784E-4 26-27 0.0 0.0 0.0 5.114762879592902E-4 5.424748508659138E-4 28-29 3.099856290662365E-5 0.0 0.0 0.002278394373636838 5.579741323192257E-4 30-31 3.099856290662365E-5 0.0 0.0 0.007377657971776428 5.734734137725375E-4 32-33 3.099856290662365E-5 0.0 0.0 0.017018211035736384 6.19971258132473E-4 34-35 6.19971258132473E-5 0.0 0.0 0.032331501111608464 6.509698210390967E-4 36-37 6.19971258132473E-5 0.0 0.0 0.05809130688701272 6.974676653990321E-4 38-39 6.19971258132473E-5 0.0 0.0 0.09721149327517176 7.284662283056557E-4 40-41 6.19971258132473E-5 0.0 0.0 0.14322885991005457 7.439655097589675E-4 42-43 7.749640726655912E-5 0.0 0.0 0.1921445921767067 7.439655097589675E-4 44-45 9.299568871987094E-5 0.0 0.0 0.25028239690807935 7.439655097589675E-4 46-47 9.299568871987094E-5 0.0 0.0 0.32359399818224427 7.594647912122793E-4 48-49 9.299568871987094E-5 0.0 0.0 0.4053062100041042 8.83459042838774E-4 50-51 9.299568871987094E-5 0.0 0.0 0.4939311013541412 9.299568871987095E-4 52-53 9.299568871987094E-5 0.0 0.0 0.583392953902657 9.454561686520213E-4 54-55 9.299568871987094E-5 0.0 0.0 0.68706764754386 9.609554501053331E-4 56-57 1.239942516264946E-4 0.0 0.0 0.8143167482755499 9.609554501053331E-4 58-59 1.239942516264946E-4 0.0 0.0 0.9513613948857331 9.609554501053331E-4 60-61 1.239942516264946E-4 0.0 0.0 1.1010999530061787 9.609554501053331E-4 62-63 1.239942516264946E-4 0.0 0.0 1.2596421029921052 9.919540130119567E-4 64-65 1.239942516264946E-4 0.0 0.0 1.4299327083196425 9.919540130119567E-4 66-67 1.5499281453311824E-4 0.0 0.0 1.6269440748726889 9.919540130119567E-4 68-69 1.5499281453311824E-4 0.0 0.0 1.8541170431338803 9.919540130119567E-4 70-71 1.5499281453311824E-4 0.0 0.0 2.1024930284232024 0.0010229525759185803 72-73 1.5499281453311824E-4 0.0 0.0 2.3539998685660932 0.0011004489831851396 74-75 1.5499281453311824E-4 0.0 0.0 2.6225559183076275 0.001146946827545075 76-77 1.5499281453311824E-4 0.0 0.0 2.914298893103316 0.001146946827545075 78-79 1.5499281453311824E-4 0.0 0.0 3.260692334303382 0.0011779453904516987 80-81 1.5499281453311824E-4 0.0 0.0 3.6369993887083396 0.001224443234811634 82-83 1.5499281453311824E-4 0.0 0.0 4.015584837486934 0.001332938204984817 84-85 1.5499281453311824E-4 0.0 0.0 4.395394729500341 0.001332938204984817 86-87 1.5499281453311824E-4 0.0 0.0 4.804668755556492 0.0013484374864381288 88-89 1.5499281453311824E-4 0.0 0.0 5.260735112320193 0.0013794360493447524 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8665 0.0 22.765745 1 TACATGG 8510 0.0 22.360987 2 ACATGGG 8555 0.0 20.690212 3 GTAAGCG 510 0.0 20.00558 94-95 GAGTACT 7515 0.0 19.481958 12-13 GTACTTT 7670 0.0 18.964655 14-15 CATGGGG 6220 0.0 18.539244 4 AGAGTAC 11390 0.0 18.4996 10-11 GAGTACA 6885 0.0 17.855246 1 AGTACTT 7910 0.0 17.849125 12-13 GTGTACG 375 1.6552804E-10 17.788809 1 TACACTG 2315 0.0 16.398916 5 GTATAGC 1285 0.0 16.315472 1 AGTACAT 6750 0.0 16.239937 2 CCTATAC 800 0.0 16.015848 3 GTACTAG 420 8.258212E-10 15.882864 1 TTTGCGT 640 0.0 15.570963 3 GTGTAGG 1270 0.0 15.007432 1 TACTTTT 9180 0.0 14.785402 14-15 GTACCGA 290 1.8832421E-5 14.727217 6 >>END_MODULE