FastQCFastQC Report
Fri 27 May 2016
SRR938982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230162
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT144980.448831978086548No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74700.23125775115922978No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61050.18899980867832636No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58330.18057917838176538No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55400.17150842589319049No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48820.15113793054342164No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40980.12686670204156944No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT35910.11117089483437673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG86850.023.693371
TACATGG86100.022.7034992
ACATGGG88650.020.9292283
TTTGCGT6550.020.2331093
GAGTACT68250.019.1060812-13
AGAGTAC106600.018.44844210-11
GTATTAG9850.018.2917121
GTACTTT72900.017.757814-15
CATGGGG59050.017.6339134
GTGTAGC18850.017.1042691
AGTACTT72700.016.96000312-13
GAGTACA68150.016.4192431
GGACCGT4106.4574124E-1016.160316
CTACACT13750.015.8343784
CCACGCG1800.00268300715.7770069
GTGTAGG11500.015.2549531
AGTACAT69100.015.0007152
GTACTAG4753.1468517E-1014.9728561
GTATAGG8000.014.8168871
CGTAGAC2308.209481E-414.4050933
Produced by FastQC (version 0.11.5)